Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068126.t1 | KEH20153.1 | 97.5 | 121 | 3 | 0 | 16 | 136 | 143 | 263 | 5.60E-59 | 236.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068126.t1 | A0A072TTG7 | 97.5 | 121 | 3 | 0 | 16 | 136 | 143 | 263 | 4.0e-59 | 236.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene068126 | 0.810097 | 1.37E-50 | -1.69E-46 |
| MS.gene049153 | MS.gene068126 | 0.815061 | 1.12E-51 | -1.69E-46 |
| MS.gene051983 | MS.gene068126 | 0.824602 | 7.39E-54 | -1.69E-46 |
| MS.gene053494 | MS.gene068126 | 0.818672 | 1.73E-52 | -1.69E-46 |
| MS.gene054349 | MS.gene068126 | 0.816176 | 6.33E-52 | -1.69E-46 |
| MS.gene056723 | MS.gene068126 | 0.823505 | 1.34E-53 | -1.69E-46 |
| MS.gene056979 | MS.gene068126 | 0.806454 | 8.16E-50 | -1.69E-46 |
| MS.gene057396 | MS.gene068126 | 0.816572 | 5.16E-52 | -1.69E-46 |
| MS.gene058142 | MS.gene068126 | 0.834234 | 3.37E-56 | -1.69E-46 |
| MS.gene058949 | MS.gene068126 | 0.820216 | 7.71E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068126.t1 | MTR_8g069450 | 97.059 | 136 | 4 | 0 | 1 | 136 | 128 | 263 | 2.28e-95 | 275 |
| MS.gene068126.t1 | MTR_8g069450 | 96.154 | 130 | 5 | 0 | 1 | 130 | 128 | 257 | 3.33e-89 | 259 |
| MS.gene068126.t1 | MTR_8g069450 | 96.875 | 128 | 4 | 0 | 1 | 128 | 128 | 255 | 5.81e-87 | 256 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068126.t1 | AT3G26580 | 81.250 | 128 | 24 | 0 | 1 | 128 | 154 | 281 | 2.71e-75 | 228 |
Find 39 sgRNAs with CRISPR-Local
Find 94 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATACCAAGATCTACATTATT+TGG | 0.191918 | 8.4:-34418753 | MS.gene068126:CDS |
| GCAATCCTTATGGCGATTAT+TGG | 0.215703 | 8.4:+34418567 | None:intergenic |
| TGCAGAACTTCGTTACATTT+TGG | 0.260230 | 8.4:-34418485 | MS.gene068126:CDS |
| GATTTGGGTAAGAAAGCTTA+TGG | 0.281355 | 8.4:-34418828 | MS.gene068126:CDS |
| GTGAATTTCAGATACAAATA+TGG | 0.289294 | 8.4:-34418609 | MS.gene068126:intron |
| AGCTCAGTTGATGTATGATT+TGG | 0.292561 | 8.4:-34418844 | MS.gene068126:CDS |
| AAGAGAAGAGGATGAGAGTT+AGG | 0.351749 | 8.4:-34420103 | None:intergenic |
| GTATAGTCACCATCTCTTCT+TGG | 0.352541 | 8.4:+34418440 | None:intergenic |
| TCTTGGGTTATCTTGAGCTT+TGG | 0.386466 | 8.4:+34418457 | None:intergenic |
| TATAGTCACCATCTCTTCTT+GGG | 0.408773 | 8.4:+34418441 | None:intergenic |
| TGACGTTTGATGCTAATACT+AGG | 0.431805 | 8.4:+34418511 | None:intergenic |
| GCTCAGTTGATGTATGATTT+GGG | 0.432267 | 8.4:-34418843 | MS.gene068126:CDS |
| AAAGAGCAATGCAATCCTTA+TGG | 0.434951 | 8.4:+34418557 | None:intergenic |
| CCACCAAATAATGTAGATCT+TGG | 0.439479 | 8.4:+34418750 | None:intergenic |
| AGTTAGGAAAGAGCTTGAAA+AGG | 0.442833 | 8.4:-34420087 | MS.gene068126:intron |
| ATTTGGGTAAGAAAGCTTAT+GGG | 0.445943 | 8.4:-34418827 | MS.gene068126:CDS |
| ATGGCTGGCTATGGCTTATG+AGG | 0.453962 | 8.4:-34418590 | MS.gene068126:CDS |
| GTCATAACTAGAACCAATCA+AGG | 0.468929 | 8.4:+34418417 | None:intergenic |
| ATGGTGACTATACCCTTGAT+TGG | 0.471938 | 8.4:-34418430 | MS.gene068126:CDS |
| CGTGCCGTTGAGTTTCTTGA+AGG | 0.493680 | 8.4:-34418789 | MS.gene068126:CDS |
| GACGTTTGATGCTAATACTA+GGG | 0.495643 | 8.4:+34418512 | None:intergenic |
| ATCTACATTATTTGGTGGTG+AGG | 0.501114 | 8.4:-34418745 | MS.gene068126:intron |
| TGATTGGTTCTAGTTATGAC+AGG | 0.513314 | 8.4:-34418414 | MS.gene068126:CDS |
| GAATCAGGTGGCTAAGGAGC+AGG | 0.526095 | 8.4:-34418883 | MS.gene068126:intron |
| CCAAGATCTACATTATTTGG+TGG | 0.527272 | 8.4:-34418750 | MS.gene068126:CDS |
| GCTTATGGGAGAGGCACGTA+TGG | 0.534948 | 8.4:-34418813 | MS.gene068126:CDS |
| TAAGGAGCAGGCAGAGAGGA+GGG | 0.539752 | 8.4:-34418871 | MS.gene068126:CDS |
| GGTAAGAAAGCTTATGGGAG+AGG | 0.539832 | 8.4:-34418822 | MS.gene068126:CDS |
| TGAGGCCAATAATCGCCATA+AGG | 0.547038 | 8.4:-34418572 | MS.gene068126:CDS |
| TAATGTAGATCTTGGTATGA+TGG | 0.552370 | 8.4:+34418758 | None:intergenic |
| ATTTCAGATACAAATATGGC+TGG | 0.553752 | 8.4:-34418605 | MS.gene068126:intron |
| GATACAAATATGGCTGGCTA+TGG | 0.569208 | 8.4:-34418599 | MS.gene068126:CDS |
| CAAGATAACCCAAGAAGAGA+TGG | 0.579385 | 8.4:-34418449 | MS.gene068126:CDS |
| CTAAGGAGCAGGCAGAGAGG+AGG | 0.584864 | 8.4:-34418872 | MS.gene068126:CDS |
| TTTGACGAATCAGGTGGCTA+AGG | 0.599329 | 8.4:-34418889 | MS.gene068126:intron |
| TGCACCTTCAAGAAACTCAA+CGG | 0.650398 | 8.4:+34418785 | None:intergenic |
| ATCATACATCAACTGAGCTG+TGG | 0.650968 | 8.4:+34418848 | None:intergenic |
| TCATAACTAGAACCAATCAA+GGG | 0.666833 | 8.4:+34418418 | None:intergenic |
| TGGCTAAGGAGCAGGCAGAG+AGG | 0.675876 | 8.4:-34418875 | MS.gene068126:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAAGCAATTTTTTATTTTTT+AGG | - | chr8.4:34418907-34418926 | MS.gene068126:intron | 10.0% |
| !!! | AATTTTGTAGTTTTTGTTTT+TGG | - | chr8.4:34418545-34418564 | MS.gene068126:CDS | 15.0% |
| !! | CATATACATCATTTATTCAA+CGG | - | chr8.4:34418969-34418988 | MS.gene068126:intron | 20.0% |
| !! | GATGTATATGATCTATATTA+TGG | + | chr8.4:34418959-34418978 | None:intergenic | 20.0% |
| !! | TAAACAGTAGTTTAGTATTT+AGG | + | chr8.4:34418576-34418595 | None:intergenic | 20.0% |
| !! | TAACAAGGATAATTTCTTAA+AGG | - | chr8.4:34418594-34418613 | MS.gene068126:CDS | 20.0% |
| !! | TTGCTTACAAATATTTGTTT+AGG | + | chr8.4:34419485-34419504 | None:intergenic | 20.0% |
| !!! | AATAACTATTCTTGGTTTTT+TGG | - | chr8.4:34419306-34419325 | MS.gene068126:intron | 20.0% |
| !!! | AGATGGATTGTAATATTATA+TGG | - | chr8.4:34419278-34419297 | MS.gene068126:intron | 20.0% |
| !!! | GATTCTTATTTAGTTAAGAA+TGG | + | chr8.4:34419768-34419787 | None:intergenic | 20.0% |
| !!! | TTTTTGACTAATTTGTTGAT+TGG | - | chr8.4:34419066-34419085 | MS.gene068126:intron | 20.0% |
| ! | ACAGTTATGAGAAAATCATT+TGG | + | chr8.4:34418654-34418673 | None:intergenic | 25.0% |
| ! | ATACCAAGATCTACATTATT+TGG | - | chr8.4:34419729-34419748 | MS.gene068126:intron | 25.0% |
| ! | CAGTTATGAGAAAATCATTT+GGG | + | chr8.4:34418653-34418672 | None:intergenic | 25.0% |
| ! | CATTGAATAAATGATGTATG+TGG | - | chr8.4:34418932-34418951 | MS.gene068126:intron | 25.0% |
| ! | GTGAATTTCAGATACAAATA+TGG | - | chr8.4:34419873-34419892 | MS.gene068126:intron | 25.0% |
| ! | GTGTATGTAAAGAAAACAAA+AGG | + | chr8.4:34419167-34419186 | None:intergenic | 25.0% |
| ! | TACTAAACTACTGTTTAACA+AGG | - | chr8.4:34418579-34418598 | MS.gene068126:CDS | 25.0% |
| !!! | GTTTTCATACTTTTTGTTTG+AGG | - | chr8.4:34419361-34419380 | MS.gene068126:intron | 25.0% |
| !!! | TGTAGTTTTTGTTTTTGGAA+AGG | - | chr8.4:34418550-34418569 | MS.gene068126:CDS | 25.0% |
| AACTATTACATATGAGACAG+AGG | + | chr8.4:34418500-34418519 | None:intergenic | 30.0% | |
| ATTTCAGATACAAATATGGC+TGG | - | chr8.4:34419877-34419896 | MS.gene068126:intron | 30.0% | |
| ATTTGGGTAAGAAAGCTTAT+GGG | - | chr8.4:34419655-34419674 | MS.gene068126:intron | 30.0% | |
| CTAGAAGTGAAACATTAAGA+TGG | - | chr8.4:34419261-34419280 | MS.gene068126:intron | 30.0% | |
| TAATGTAGATCTTGGTATGA+TGG | + | chr8.4:34419727-34419746 | None:intergenic | 30.0% | |
| TCATAACTAGAACCAATCAA+GGG | + | chr8.4:34420067-34420086 | None:intergenic | 30.0% | |
| TGCTTATAATTGAGACTAGA+GGG | + | chr8.4:34418893-34418912 | None:intergenic | 30.0% | |
| TGGTGCATAATAACTATTCT+TGG | - | chr8.4:34419298-34419317 | MS.gene068126:intron | 30.0% | |
| TGTCAGTTGTCTAATGATAA+AGG | + | chr8.4:34419222-34419241 | None:intergenic | 30.0% | |
| TTGCTTATAATTGAGACTAG+AGG | + | chr8.4:34418894-34418913 | None:intergenic | 30.0% | |
| ! | ATCTTTTTGTTGGGACTTAT+GGG | + | chr8.4:34418848-34418867 | None:intergenic | 30.0% |
| AAAACCCGAAAGCAGTTATA+TGG | + | chr8.4:34418744-34418763 | None:intergenic | 35.0% | |
| AAAGAGCAATGCAATCCTTA+TGG | + | chr8.4:34419928-34419947 | None:intergenic | 35.0% | |
| ACAAAGTTTCACGATAACAG+TGG | + | chr8.4:34419136-34419155 | None:intergenic | 35.0% | |
| ACTCGTTGACATATTGTTTG+TGG | - | chr8.4:34419570-34419589 | MS.gene068126:intron | 35.0% | |
| ACTGTTATCGTGAAACTTTG+TGG | - | chr8.4:34419135-34419154 | MS.gene068126:intron | 35.0% | |
| AGTTAGGAAAGAGCTTGAAA+AGG | - | chr8.4:34418395-34418414 | MS.gene068126:CDS | 35.0% | |
| CAAAGTTTCACGATAACAGT+GGG | + | chr8.4:34419135-34419154 | None:intergenic | 35.0% | |
| CACAAGTATGTCTAGATACT+AGG | + | chr8.4:34418776-34418795 | None:intergenic | 35.0% | |
| CCAAGATCTACATTATTTGG+TGG | - | chr8.4:34419732-34419751 | MS.gene068126:intron | 35.0% | |
| CCACCAAATAATGTAGATCT+TGG | + | chr8.4:34419735-34419754 | None:intergenic | 35.0% | |
| GACGTTTGATGCTAATACTA+GGG | + | chr8.4:34419973-34419992 | None:intergenic | 35.0% | |
| GATTTGGGTAAGAAAGCTTA+TGG | - | chr8.4:34419654-34419673 | MS.gene068126:intron | 35.0% | |
| GTCATAACTAGAACCAATCA+AGG | + | chr8.4:34420068-34420087 | None:intergenic | 35.0% | |
| TATAGTCACCATCTCTTCTT+GGG | + | chr8.4:34420044-34420063 | None:intergenic | 35.0% | |
| TGACGTTTGATGCTAATACT+AGG | + | chr8.4:34419974-34419993 | None:intergenic | 35.0% | |
| ! | ACTCTCTCGATCTTTTTGTT+GGG | + | chr8.4:34418857-34418876 | None:intergenic | 35.0% |
| ! | AGCTCAGTTGATGTATGATT+TGG | - | chr8.4:34419638-34419657 | MS.gene068126:intron | 35.0% |
| ! | GATCTTTTTGTTGGGACTTA+TGG | + | chr8.4:34418849-34418868 | None:intergenic | 35.0% |
| ! | GCTCAGTTGATGTATGATTT+GGG | - | chr8.4:34419639-34419658 | MS.gene068126:intron | 35.0% |
| ! | TGATCCTCTCTAGTGTTTTA+AGG | - | chr8.4:34418819-34418838 | MS.gene068126:CDS | 35.0% |
| ! | TGCAGAACTTCGTTACATTT+TGG | - | chr8.4:34419997-34420016 | MS.gene068126:intron | 35.0% |
| !! | ACCATTATTTTTCTCCTCTC+GGG | - | chr8.4:34419524-34419543 | MS.gene068126:intron | 35.0% |
| !! | AGACAGAGGAGTACTAATTA+AGG | + | chr8.4:34418486-34418505 | None:intergenic | 35.0% |
| !! | ATCTACATTATTTGGTGGTG+AGG | - | chr8.4:34419737-34419756 | MS.gene068126:intron | 35.0% |
| !! | GACCATTATTTTTCTCCTCT+CGG | - | chr8.4:34419523-34419542 | MS.gene068126:intron | 35.0% |
| !! | TGATTGGTTCTAGTTATGAC+AGG | - | chr8.4:34420068-34420087 | MS.gene068126:intron | 35.0% |
| !! | TTCACTTCTAGTGACTTGTT+AGG | + | chr8.4:34419252-34419271 | None:intergenic | 35.0% |
| !!! | ATATAACTGCTTTCGGGTTT+TGG | - | chr8.4:34418743-34418762 | MS.gene068126:intron | 35.0% |
| !!! | TATAACTGCTTTCGGGTTTT+GGG | - | chr8.4:34418744-34418763 | MS.gene068126:intron | 35.0% |
| ACCCGAGAGGAGAAAAATAA+TGG | + | chr8.4:34419528-34419547 | None:intergenic | 40.0% | |
| ATCATACATCAACTGAGCTG+TGG | + | chr8.4:34419637-34419656 | None:intergenic | 40.0% | |
| ATGGTGACTATACCCTTGAT+TGG | - | chr8.4:34420052-34420071 | MS.gene068126:intron | 40.0% | |
| CAAGATAACCCAAGAAGAGA+TGG | - | chr8.4:34420033-34420052 | MS.gene068126:intron | 40.0% | |
| GAAGCCTTAAAACACTAGAG+AGG | + | chr8.4:34418826-34418845 | None:intergenic | 40.0% | |
| GCAATCCTTATGGCGATTAT+TGG | + | chr8.4:34419918-34419937 | None:intergenic | 40.0% | |
| GTATAGTCACCATCTCTTCT+TGG | + | chr8.4:34420045-34420064 | None:intergenic | 40.0% | |
| GTGGAGGGTGATGATTAAAT+TGG | - | chr8.4:34419546-34419565 | MS.gene068126:intron | 40.0% | |
| TCTTGGGTTATCTTGAGCTT+TGG | + | chr8.4:34420028-34420047 | None:intergenic | 40.0% | |
| TGCACCTTCAAGAAACTCAA+CGG | + | chr8.4:34419700-34419719 | None:intergenic | 40.0% | |
| TGTTTGTGGTTTGACGAATC+AGG | - | chr8.4:34419584-34419603 | MS.gene068126:intron | 40.0% | |
| ! | CACTCTCTCGATCTTTTTGT+TGG | + | chr8.4:34418858-34418877 | None:intergenic | 40.0% |
| ! | GATACAAATATGGCTGGCTA+TGG | - | chr8.4:34419883-34419902 | MS.gene068126:intron | 40.0% |
| !! | ATTATTTTTCTCCTCTCGGG+TGG | - | chr8.4:34419527-34419546 | MS.gene068126:intron | 40.0% |
| CCAACGAGACATCGCTTAAT+TGG | + | chr8.4:34419189-34419208 | None:intergenic | 45.0% | |
| CCAATTAAGCGATGTCTCGT+TGG | - | chr8.4:34419186-34419205 | MS.gene068126:intron | 45.0% | |
| CCCTGCCATATAACTGCTTT+CGG | - | chr8.4:34418736-34418755 | MS.gene068126:intron | 45.0% | |
| CCGAAAGCAGTTATATGGCA+GGG | + | chr8.4:34418739-34418758 | None:intergenic | 45.0% | |
| CCTGCCATATAACTGCTTTC+GGG | - | chr8.4:34418737-34418756 | MS.gene068126:intron | 45.0% | |
| GGTAAGAAAGCTTATGGGAG+AGG | - | chr8.4:34419660-34419679 | MS.gene068126:intron | 45.0% | |
| TGAGGCCAATAATCGCCATA+AGG | - | chr8.4:34419910-34419929 | MS.gene068126:intron | 45.0% | |
| TTGTGGTTTGACGAATCAGG+TGG | - | chr8.4:34419587-34419606 | MS.gene068126:intron | 45.0% | |
| TTTGACGAATCAGGTGGCTA+AGG | - | chr8.4:34419593-34419612 | MS.gene068126:intron | 45.0% | |
| CCCGAAAGCAGTTATATGGC+AGG | + | chr8.4:34418740-34418759 | None:intergenic | 50.0% | |
| ! | ATGGCTGGCTATGGCTTATG+AGG | - | chr8.4:34419892-34419911 | MS.gene068126:intron | 50.0% |
| ! | ATTTTTCTCCTCTCGGGTGG+AGG | - | chr8.4:34419530-34419549 | MS.gene068126:intron | 50.0% |
| ! | CGTGCCGTTGAGTTTCTTGA+AGG | - | chr8.4:34419693-34419712 | MS.gene068126:intron | 50.0% |
| ! | TTTTTCTCCTCTCGGGTGGA+GGG | - | chr8.4:34419531-34419550 | MS.gene068126:intron | 50.0% |
| GAATCAGGTGGCTAAGGAGC+AGG | - | chr8.4:34419599-34419618 | MS.gene068126:intron | 55.0% | |
| TAAGGAGCAGGCAGAGAGGA+GGG | - | chr8.4:34419611-34419630 | MS.gene068126:intron | 55.0% | |
| ! | GCTTATGGGAGAGGCACGTA+TGG | - | chr8.4:34419669-34419688 | MS.gene068126:intron | 55.0% |
| ATCATCACCCTCCACCCGAG+AGG | + | chr8.4:34419541-34419560 | None:intergenic | 60.0% | |
| CTAAGGAGCAGGCAGAGAGG+AGG | - | chr8.4:34419610-34419629 | MS.gene068126:intron | 60.0% | |
| TGGCTAAGGAGCAGGCAGAG+AGG | - | chr8.4:34419607-34419626 | MS.gene068126:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 34418390 | 34420114 | 34418390 | ID=MS.gene068126 |
| chr8.4 | mRNA | 34418390 | 34420114 | 34418390 | ID=MS.gene068126.t1;Parent=MS.gene068126 |
| chr8.4 | exon | 34420088 | 34420114 | 34420088 | ID=MS.gene068126.t1.exon1;Parent=MS.gene068126.t1 |
| chr8.4 | CDS | 34420088 | 34420114 | 34420088 | ID=cds.MS.gene068126.t1;Parent=MS.gene068126.t1 |
| chr8.4 | exon | 34418746 | 34418898 | 34418746 | ID=MS.gene068126.t1.exon2;Parent=MS.gene068126.t1 |
| chr8.4 | CDS | 34418746 | 34418898 | 34418746 | ID=cds.MS.gene068126.t1;Parent=MS.gene068126.t1 |
| chr8.4 | exon | 34418390 | 34418620 | 34418390 | ID=MS.gene068126.t1.exon3;Parent=MS.gene068126.t1 |
| chr8.4 | CDS | 34418390 | 34418620 | 34418390 | ID=cds.MS.gene068126.t1;Parent=MS.gene068126.t1 |
| Gene Sequence |
| Protein sequence |
