AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068488

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068488.t1 MTR_0040s0140 96.094 256 10 0 1 256 1 256 0.0 518
MS.gene068488.t1 MTR_2g090255 78.968 252 52 1 6 256 1 252 3.05e-158 439
MS.gene068488.t1 MTR_5g076950 60.619 226 88 1 31 256 40 264 1.71e-98 289
MS.gene068488.t1 MTR_5g079950 51.304 230 112 0 27 256 28 257 5.84e-88 261
MS.gene068488.t1 MTR_7g061720 50.435 230 114 0 27 256 27 256 1.04e-87 261
MS.gene068488.t1 MTR_5g075320 48.996 249 125 1 8 256 5 251 3.53e-87 259
MS.gene068488.t1 MTR_2g097030 50.435 230 114 0 27 256 29 258 1.31e-86 258
MS.gene068488.t1 MTR_3g094060 50.000 246 121 1 11 256 10 253 3.68e-86 257
MS.gene068488.t1 MTR_3g064510 55.508 236 92 6 31 256 52 284 1.44e-85 256
MS.gene068488.t1 MTR_5g041700 50.000 244 120 1 13 256 6 247 1.75e-85 255
MS.gene068488.t1 MTR_3g435430 52.632 228 106 1 29 256 22 247 3.23e-85 254
MS.gene068488.t1 MTR_3g094040 52.889 225 104 1 32 256 31 253 3.89e-85 254
MS.gene068488.t1 MTR_4g081950 50.000 244 120 1 13 256 42 283 4.32e-84 253
MS.gene068488.t1 MTR_4g063975 48.438 256 124 2 9 256 1 256 5.74e-84 251
MS.gene068488.t1 MTR_3g466790 53.778 225 102 1 32 256 32 254 6.99e-84 251
MS.gene068488.t1 MTR_7g013140 49.383 243 118 2 12 254 11 248 7.07e-84 251
MS.gene068488.t1 MTR_3g466760 53.333 225 103 1 32 256 38 260 4.13e-83 249
MS.gene068488.t1 MTR_7g013150 47.934 242 121 2 13 254 14 250 2.04e-80 242
MS.gene068488.t1 MTR_7g011060 48.387 248 113 3 14 256 3 240 4.54e-80 241
MS.gene068488.t1 MTR_8g058990 46.032 252 133 2 2 253 1 249 1.07e-78 238
MS.gene068488.t1 MTR_8g464350 46.939 245 128 1 8 252 7 249 2.59e-77 234
MS.gene068488.t1 MTR_4g124340 50.667 225 108 2 32 255 61 283 6.92e-77 234
MS.gene068488.t1 MTR_7g013120 50.877 228 109 2 29 255 26 251 7.55e-77 233
MS.gene068488.t1 MTR_1g085460 49.356 233 114 3 25 254 23 254 3.81e-76 231
MS.gene068488.t1 MTR_7g111010 46.559 247 124 4 13 252 4 249 6.77e-75 228
MS.gene068488.t1 MTR_7g110990 49.554 224 109 3 32 252 12 234 1.90e-74 226
MS.gene068488.t1 MTR_6g012520 48.606 251 114 3 11 256 2 242 7.44e-73 223
MS.gene068488.t1 MTR_1g026020 42.353 255 139 5 4 254 2 252 4.97e-70 216
MS.gene068488.t1 MTR_5g011210 41.129 248 144 2 8 254 13 259 4.86e-67 209
MS.gene068488.t1 MTR_4g102450 43.568 241 134 2 13 252 17 256 8.12e-67 208
MS.gene068488.t1 MTR_0008s0120 51.852 108 52 0 146 253 88 195 4.57e-34 122
MS.gene068488.t1 MTR_0008s0120 39.326 89 54 0 1 89 1 89 4.42e-14 69.3
MS.gene068488.t1 MTR_6g012500 54.000 100 46 0 157 256 181 280 1.35e-33 123
MS.gene068488.t1 MTR_2g090270 31.837 245 139 9 23 254 26 255 5.18e-30 113
MS.gene068488.t1 MTR_2g090265 31.381 239 136 10 29 254 30 253 4.32e-28 108
MS.gene068488.t1 MTR_0122s0020 32.773 238 134 9 29 254 38 261 5.11e-28 108
MS.gene068488.t1 MTR_4g028640 31.513 238 132 10 29 252 39 259 1.83e-24 99.0
MS.gene068488.t1 MTR_0118s0070 29.130 230 135 11 37 251 53 269 8.35e-19 83.6
MS.gene068488.t1 MTR_0809s0010 29.600 250 142 13 18 252 35 265 2.27e-17 79.7
MS.gene068488.t1 MTR_0122s0020 32.597 181 97 8 29 198 38 204 3.26e-16 75.5
MS.gene068488.t1 MTR_5g013440 28.194 227 146 9 35 254 32 248 1.07e-13 69.3
MS.gene068488.t1 MTR_1g085365 51.786 56 27 0 201 256 4 59 2.60e-12 60.8
MS.gene068488.t1 MTR_4g075790 26.106 226 132 10 43 252 32 238 3.48e-12 65.1
MS.gene068488.t1 MTR_4g099370 25.664 226 151 8 34 252 30 245 3.34e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068488.t1 AT4G38210 64.898 245 82 2 14 256 12 254 6.38e-119 340
MS.gene068488.t1 AT3G03220 57.965 226 94 1 31 256 39 263 5.80e-94 277
MS.gene068488.t1 AT2G39700 47.843 255 133 0 2 256 1 255 7.05e-90 266
MS.gene068488.t1 AT3G55500 50.602 249 119 1 12 256 10 258 1.76e-89 266
MS.gene068488.t1 AT5G02260 48.000 250 125 1 12 256 7 256 1.33e-88 263
MS.gene068488.t1 AT2G37640 49.402 251 119 1 14 256 10 260 1.10e-87 261
MS.gene068488.t1 AT5G05290 51.271 236 112 2 22 256 22 255 1.03e-85 256
MS.gene068488.t1 AT2G03090 49.609 256 125 3 1 256 1 252 9.43e-85 253
MS.gene068488.t1 AT2G40610 50.201 249 121 3 9 256 7 253 1.85e-84 253
MS.gene068488.t1 AT1G69530 50.202 247 119 2 12 256 5 249 3.73e-84 252
MS.gene068488.t1 AT1G69530 50.202 247 119 2 12 256 5 249 3.73e-84 252
MS.gene068488.t1 AT5G56320 48.560 243 123 1 14 256 11 251 4.68e-84 251
MS.gene068488.t1 AT5G56320 48.560 243 123 1 14 256 11 251 4.68e-84 251
MS.gene068488.t1 AT2G37640 53.810 210 97 0 47 256 26 235 1.25e-83 250
MS.gene068488.t1 AT5G56320 48.560 243 123 1 14 256 11 251 2.31e-83 251
MS.gene068488.t1 AT1G69530 50.413 242 116 2 12 251 5 244 3.02e-82 248
MS.gene068488.t1 AT2G28950 46.850 254 134 1 4 256 2 255 2.31e-81 245
MS.gene068488.t1 AT5G39280 50.885 226 107 2 29 254 51 272 1.74e-78 238
MS.gene068488.t1 AT5G39300 50.000 226 109 2 29 254 52 273 2.26e-77 235
MS.gene068488.t1 AT5G39270 50.442 226 108 2 29 254 55 276 1.24e-76 234
MS.gene068488.t1 AT1G26770 48.207 251 127 2 6 256 1 248 3.16e-76 231
MS.gene068488.t1 AT1G26770 48.016 252 128 2 5 256 10 258 4.54e-76 231
MS.gene068488.t1 AT5G39290 50.000 226 109 2 29 254 55 276 6.71e-76 232
MS.gene068488.t1 AT3G29030 46.768 263 118 5 6 256 1 253 9.77e-76 231
MS.gene068488.t1 AT1G62980 43.210 243 136 2 13 254 12 253 2.62e-73 224
MS.gene068488.t1 AT4G01630 46.875 224 115 3 32 252 28 250 4.14e-70 216
MS.gene068488.t1 AT5G39260 43.922 255 128 4 6 254 14 259 5.44e-70 216
MS.gene068488.t1 AT1G20190 44.397 232 124 3 24 252 18 247 1.19e-69 215
MS.gene068488.t1 AT5G39310 48.214 224 111 3 32 254 90 309 2.73e-69 216
MS.gene068488.t1 AT1G69530 44.130 247 104 3 12 256 5 219 1.14e-68 211
MS.gene068488.t1 AT1G69530 49.510 204 99 2 12 213 5 206 2.00e-67 209
MS.gene068488.t1 AT1G12560 43.033 244 134 4 12 252 15 256 9.27e-67 208
MS.gene068488.t1 AT3G15370 44.747 257 130 6 6 256 1 251 1.75e-66 207
MS.gene068488.t1 AT3G15370 41.115 287 127 8 6 256 1 281 8.56e-62 196
MS.gene068488.t1 AT1G20190 46.561 189 96 3 67 252 2 188 2.07e-59 187
MS.gene068488.t1 AT4G28250 32.780 241 131 11 29 254 33 257 1.29e-24 99.4
MS.gene068488.t1 AT1G65680 30.242 248 142 9 22 252 35 268 1.04e-23 97.1
MS.gene068488.t1 AT2G20750 32.271 251 142 11 18 254 28 264 3.81e-23 95.5
MS.gene068488.t1 AT4G28250 32.500 240 127 11 29 254 33 251 1.22e-22 94.0
MS.gene068488.t1 AT2G45110 29.741 232 136 11 35 252 36 254 1.87e-17 79.7
MS.gene068488.t1 AT1G65681 28.182 220 133 9 45 252 11 217 6.86e-17 77.4
MS.gene068488.t1 AT3G60570 25.483 259 159 10 15 254 11 254 8.34e-15 72.4
MS.gene068488.t1 AT2G20750 32.804 189 103 8 18 195 28 203 3.07e-14 70.1
MS.gene068488.t1 AT3G60570 25.792 221 136 9 47 254 1 206 1.96e-11 62.4
MS.gene068488.t1 AT4G17030 25.969 258 151 11 13 252 9 244 2.28e-11 62.8

Find 60 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTTCTGTCCTCCTAATTT+TGG 0.175976 4.2:-58316334 MS.gene068488:CDS
GCACCTCCAAACTGGCAATT+TGG 0.255098 4.2:-58315536 MS.gene068488:CDS
TAGAGATCCTTCTGTGTCTT+TGG 0.259434 4.2:+58316738 None:intergenic
TTTGGTCTCTCAGTTGATTA+TGG 0.289936 4.2:-58316316 MS.gene068488:CDS
GGCTACCAATGGCAAGGAAT+TGG 0.308760 4.2:-58315655 MS.gene068488:CDS
GTTTGTCCAAATTGCCAGTT+TGG 0.334344 4.2:+58315530 None:intergenic
TCAACTGAGAGACCAAAATT+AGG 0.348503 4.2:+58316322 None:intergenic
GCTACCAATGGCAAGGAATT+GGG 0.353144 4.2:-58315654 MS.gene068488:CDS
ACATCAGCCTTTGTCCTTTG+AGG 0.354377 4.2:-58315600 MS.gene068488:CDS
GACAACTCAAAATGTTCTCT+TGG 0.372662 4.2:+58316277 None:intergenic
GCAGAAGGAGCTTGTGGTTA+TGG 0.372980 4.2:-58316514 MS.gene068488:intron
ACAGAAGGATCTCTAATTAC+AGG 0.385780 4.2:-58316730 MS.gene068488:intron
TTATTCCCGTTCAATATAGA+AGG 0.389617 4.2:-58316213 MS.gene068488:intron
CAATTTGGACAAACATTTGA+AGG 0.398107 4.2:-58315521 MS.gene068488:CDS
CTAAATGCTGTCATCTAAGA+TGG 0.410508 4.2:-58316860 None:intergenic
TTGCTTACAAAGATCAAGAA+TGG 0.419745 4.2:-58316783 MS.gene068488:CDS
CAAGGAATTGGGGAATGAAT+TGG 0.429950 4.2:-58315643 MS.gene068488:CDS
ATCTACCTTCTATATTGAAC+GGG 0.431748 4.2:+58316208 None:intergenic
CAAGCATACACCACAAGATA+TGG 0.436575 4.2:+58316386 None:intergenic
ATAACTAATGTGGGGTTAGA+TGG 0.442300 4.2:-58315719 MS.gene068488:CDS
ACAATGTAGCACCTCCAAAC+TGG 0.451802 4.2:-58315544 MS.gene068488:CDS
AAAGTGAAGGGATCTAGAAC+AGG 0.502893 4.2:-58315680 MS.gene068488:CDS
TGCATTTGCTGAAATTGCTA+AGG 0.506123 4.2:-58316248 MS.gene068488:CDS
TCTCAGTTGATTATGGTGGT+TGG 0.506701 4.2:-58316309 MS.gene068488:CDS
CAAGTTCTGATAACTAATGT+GGG 0.508543 4.2:-58315728 MS.gene068488:CDS
TCAAGTTCTGATAACTAATG+TGG 0.509065 4.2:-58315729 MS.gene068488:CDS
AGGACAAAGGCTGATGTTGA+AGG 0.517191 4.2:+58315606 None:intergenic
GATCTTCACAAAGCTAGCTA+TGG 0.521811 4.2:-58316490 MS.gene068488:CDS
CATATCTTGTGGTGTATGCT+TGG 0.525681 4.2:-58316385 MS.gene068488:CDS
GTCTTTGGCATAAGTTGCAG+TGG 0.528308 4.2:+58316753 None:intergenic
ATTCCCCAATTCCTTGCCAT+TGG 0.528368 4.2:+58315650 None:intergenic
TATCTACCTTCTATATTGAA+CGG 0.536318 4.2:+58316207 None:intergenic
TGTCCAAATTGCCAGTTTGG+AGG 0.539604 4.2:+58315533 None:intergenic
GTGAAGTGTGAAAGAAGTGG+AGG 0.540916 4.2:-58315788 MS.gene068488:CDS
GCATTTGCTGAAATTGCTAA+GGG 0.541056 4.2:-58316247 MS.gene068488:CDS
TTTGAGGTAACTAGCAGCAC+TGG 0.546125 4.2:-58315584 MS.gene068488:CDS
TTCAACAGAGGGAGTACATG+TGG 0.560849 4.2:-58316436 MS.gene068488:CDS
AGCTATGGAAAACATAGTGT+TGG 0.560850 4.2:-58316475 MS.gene068488:CDS
CTACCAATGGCAAGGAATTG+GGG 0.568953 4.2:-58315653 MS.gene068488:CDS
GGGTTGAAATTCACAATGAG+TGG 0.572612 4.2:-58315767 MS.gene068488:CDS
GGTCTCTCAGTTGATTATGG+TGG 0.580447 4.2:-58316313 MS.gene068488:CDS
TAAATGCTGTCATCTAAGAT+GGG 0.586419 4.2:-58316859 MS.gene068488:CDS
TCAACAGAGGGAGTACATGT+GGG 0.614475 4.2:-58316435 MS.gene068488:CDS
TAAAATCAAGTAGAGAAGTG+TGG 0.616273 4.2:+58316825 None:intergenic
TGAAGTGTGAAAGAAGTGGA+GGG 0.622177 4.2:-58315787 MS.gene068488:CDS
ACTGAGAGACCAAAATTAGG+AGG 0.626412 4.2:+58316325 None:intergenic
CAGGATGGCTACCAATGGCA+AGG 0.627516 4.2:-58315661 MS.gene068488:CDS
CTAGTTACCTCAAAGGACAA+AGG 0.637645 4.2:+58315593 None:intergenic
GTGGCAGTAACAATAACAGA+AGG 0.647537 4.2:+58316358 None:intergenic
TAGAACAGGATGGCTACCAA+TGG 0.657346 4.2:-58315666 MS.gene068488:CDS
CAACAGAGGGAGTACATGTG+GGG 0.668401 4.2:-58316434 MS.gene068488:CDS
ACTTATGCCAAAGACACAGA+AGG 0.668875 4.2:-58316745 MS.gene068488:CDS
AGTGCTGCTAGTTACCTCAA+AGG 0.674064 4.2:+58315586 None:intergenic
TGAAGGGATCTAGAACAGGA+TGG 0.675002 4.2:-58315676 MS.gene068488:CDS
CATTTGCTGAAATTGCTAAG+GGG 0.677827 4.2:-58316246 MS.gene068488:CDS
AAGTTCTGATAACTAATGTG+GGG 0.688292 4.2:-58315727 MS.gene068488:CDS
ATTAGGAGGACAGAAATCAG+TGG 0.688419 4.2:+58316339 None:intergenic
TGTGATGCAGAAGGAGCTTG+TGG 0.691735 4.2:-58316520 MS.gene068488:intron
AGAGTGAAGTGTGAAAGAAG+TGG 0.695816 4.2:-58315791 MS.gene068488:CDS
GATGTGTTGACCATATCTTG+TGG 0.783191 4.2:-58316396 MS.gene068488:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTACATTTTTTTTTTTTT+TGG + chr4.2:58315810-58315829 None:intergenic 10.0%
!!! ATTTTTTTATAAGTGTTTTT+GGG - chr4.2:58316181-58316200 MS.gene068488:intron 10.0%
!! AACTTAATTAATCTCTTATT+TGG - chr4.2:58316220-58316239 MS.gene068488:CDS 15.0%
!!! GATTTTTTTATAAGTGTTTT+TGG - chr4.2:58316180-58316199 MS.gene068488:intron 15.0%
!!! TACATTTTTTTTTTTTTTGG+TGG + chr4.2:58315807-58315826 None:intergenic 15.0%
!! AAATGAAACTAAAAAACACA+AGG + chr4.2:58316372-58316391 None:intergenic 20.0%
!! ATAACATAGCTAATAAAAAG+AGG + chr4.2:58316454-58316473 None:intergenic 20.0%
!! GTTTATATAAAAACAGTTCA+AGG + chr4.2:58316305-58316324 None:intergenic 20.0%
!!! ACTGTTTTTATATAAACGTT+TGG - chr4.2:58316308-58316327 MS.gene068488:CDS 20.0%
!!! ATTTTTTTTTTTTTTGGTGG+TGG + chr4.2:58315804-58315823 None:intergenic 20.0%
!!! TGTGTTTTTTAGTTTCATTT+TGG - chr4.2:58316372-58316391 MS.gene068488:CDS 20.0%
! AAAAAGAGGCAAAAAAGATT+AGG + chr4.2:58316440-58316459 None:intergenic 25.0%
! CATAATATATGCAAGATCTA+GGG - chr4.2:58315766-58315785 MS.gene068488:CDS 25.0%
! GCACAAACAAAACAAAAATT+TGG + chr4.2:58316542-58316561 None:intergenic 25.0%
! TATCTACCTTCTATATTGAA+CGG + chr4.2:58316160-58316179 None:intergenic 25.0%
AACAGCAACATTAATAGTCT+TGG - chr4.2:58315685-58315704 MS.gene068488:CDS 30.0%
ATCTACCTTCTATATTGAAC+GGG + chr4.2:58316159-58316178 None:intergenic 30.0%
CAATTTGGACAAACATTTGA+AGG - chr4.2:58316843-58316862 MS.gene068488:CDS 30.0%
GCATAATATATGCAAGATCT+AGG - chr4.2:58315765-58315784 MS.gene068488:CDS 30.0%
TAAAATCAAGTAGAGAAGTG+TGG + chr4.2:58315542-58315561 None:intergenic 30.0%
TTATTCCCGTTCAATATAGA+AGG - chr4.2:58316151-58316170 MS.gene068488:intron 30.0%
TTGCTTACAAAGATCAAGAA+TGG - chr4.2:58315581-58315600 MS.gene068488:CDS 30.0%
! AAGTTCTGATAACTAATGTG+GGG - chr4.2:58316637-58316656 MS.gene068488:intron 30.0%
! CAAGTTCTGATAACTAATGT+GGG - chr4.2:58316636-58316655 MS.gene068488:intron 30.0%
! TCAAGTTCTGATAACTAATG+TGG - chr4.2:58316635-58316654 MS.gene068488:intron 30.0%
!!! AAGTGTTTTTGGGATGTTTT+TGG - chr4.2:58316191-58316210 MS.gene068488:intron 30.0%
!!! TTTTTTTTTTTGGTGGTGGT+CGG + chr4.2:58315800-58315819 None:intergenic 30.0%
ACAGAAGGATCTCTAATTAC+AGG - chr4.2:58315634-58315653 MS.gene068488:CDS 35.0%
AGCTATGGAAAACATAGTGT+TGG - chr4.2:58315889-58315908 MS.gene068488:intron 35.0%
ATAACTAATGTGGGGTTAGA+TGG - chr4.2:58316645-58316664 MS.gene068488:intron 35.0%
GACAACTCAAAATGTTCTCT+TGG + chr4.2:58316090-58316109 None:intergenic 35.0%
TCAACTGAGAGACCAAAATT+AGG + chr4.2:58316045-58316064 None:intergenic 35.0%
TTTGGTCTCTCAGTTGATTA+TGG - chr4.2:58316048-58316067 MS.gene068488:intron 35.0%
! AGCACAATTTTGTTCAACAG+AGG - chr4.2:58315916-58315935 MS.gene068488:intron 35.0%
! ATGTTGCTGTTATGAACCAA+AGG + chr4.2:58315676-58315695 None:intergenic 35.0%
! CATTTGCTGAAATTGCTAAG+GGG - chr4.2:58316118-58316137 MS.gene068488:intron 35.0%
! GATTTCTGTCCTCCTAATTT+TGG - chr4.2:58316030-58316049 MS.gene068488:intron 35.0%
! GCACAATTTTGTTCAACAGA+GGG - chr4.2:58315917-58315936 MS.gene068488:intron 35.0%
! GCATTTGCTGAAATTGCTAA+GGG - chr4.2:58316117-58316136 MS.gene068488:intron 35.0%
! GTTTGGCTCAATTACACATT+TGG - chr4.2:58316325-58316344 MS.gene068488:CDS 35.0%
! TGCATTTGCTGAAATTGCTA+AGG - chr4.2:58316116-58316135 MS.gene068488:intron 35.0%
!! AGTTTTTGCTGTGAAAGTGA+AGG - chr4.2:58316671-58316690 MS.gene068488:intron 35.0%
!! GTTTTTGCTGTGAAAGTGAA+GGG - chr4.2:58316672-58316691 MS.gene068488:intron 35.0%
!!! AGAACATTTTGAGTTGTCAC+TGG - chr4.2:58316092-58316111 MS.gene068488:intron 35.0%
!!! TTTTTTTTTTGGTGGTGGTC+GGG + chr4.2:58315799-58315818 None:intergenic 35.0%
ACTGAGAGACCAAAATTAGG+AGG + chr4.2:58316042-58316061 None:intergenic 40.0%
ACTTATGCCAAAGACACAGA+AGG - chr4.2:58315619-58315638 MS.gene068488:CDS 40.0%
AGAGTGAAGTGTGAAAGAAG+TGG - chr4.2:58316573-58316592 MS.gene068488:intron 40.0%
ATTAGGAGGACAGAAATCAG+TGG + chr4.2:58316028-58316047 None:intergenic 40.0%
CAAGCATACACCACAAGATA+TGG + chr4.2:58315981-58316000 None:intergenic 40.0%
CAAGGAATTGGGGAATGAAT+TGG - chr4.2:58316721-58316740 MS.gene068488:intron 40.0%
CATATCTTGTGGTGTATGCT+TGG - chr4.2:58315979-58315998 MS.gene068488:intron 40.0%
CTAGTTACCTCAAAGGACAA+AGG + chr4.2:58316774-58316793 None:intergenic 40.0%
GATCTTCACAAAGCTAGCTA+TGG - chr4.2:58315874-58315893 MS.gene068488:intron 40.0%
GGGTTGAAATTCACAATGAG+TGG - chr4.2:58316597-58316616 MS.gene068488:intron 40.0%
GTGGCAGTAACAATAACAGA+AGG + chr4.2:58316009-58316028 None:intergenic 40.0%
GTTTGTCCAAATTGCCAGTT+TGG + chr4.2:58316837-58316856 None:intergenic 40.0%
TAGAGATCCTTCTGTGTCTT+TGG + chr4.2:58315629-58315648 None:intergenic 40.0%
TCTCAGTTGATTATGGTGGT+TGG - chr4.2:58316055-58316074 MS.gene068488:intron 40.0%
TGAAGTGTGAAAGAAGTGGA+GGG - chr4.2:58316577-58316596 MS.gene068488:intron 40.0%
! AAAGTGAAGGGATCTAGAAC+AGG - chr4.2:58316684-58316703 MS.gene068488:intron 40.0%
! GATGTGTTGACCATATCTTG+TGG - chr4.2:58315968-58315987 MS.gene068488:intron 40.0%
! TACTTTTCTTGCTCCTCCTT+TGG - chr4.2:58315657-58315676 MS.gene068488:CDS 40.0%
! TTGCTGTTATGAACCAAAGG+AGG + chr4.2:58315673-58315692 None:intergenic 40.0%
!!! TTTTTTTTTGGTGGTGGTCG+GGG + chr4.2:58315798-58315817 None:intergenic 40.0%
ACAATGTAGCACCTCCAAAC+TGG - chr4.2:58316820-58316839 MS.gene068488:CDS 45.0%
ACATCAGCCTTTGTCCTTTG+AGG - chr4.2:58316764-58316783 MS.gene068488:CDS 45.0%
AGGACAAAGGCTGATGTTGA+AGG + chr4.2:58316761-58316780 None:intergenic 45.0%
ATTCCCCAATTCCTTGCCAT+TGG + chr4.2:58316717-58316736 None:intergenic 45.0%
CTACCAATGGCAAGGAATTG+GGG - chr4.2:58316711-58316730 MS.gene068488:intron 45.0%
GCTACCAATGGCAAGGAATT+GGG - chr4.2:58316710-58316729 MS.gene068488:intron 45.0%
GGTCTCTCAGTTGATTATGG+TGG - chr4.2:58316051-58316070 MS.gene068488:intron 45.0%
GTCTTTGGCATAAGTTGCAG+TGG + chr4.2:58315614-58315633 None:intergenic 45.0%
GTGAAGTGTGAAAGAAGTGG+AGG - chr4.2:58316576-58316595 MS.gene068488:intron 45.0%
TAGAACAGGATGGCTACCAA+TGG - chr4.2:58316698-58316717 MS.gene068488:intron 45.0%
TGTCCAAATTGCCAGTTTGG+AGG + chr4.2:58316834-58316853 None:intergenic 45.0%
TTGTGTGCATGTGATGCAGA+AGG - chr4.2:58315835-58315854 MS.gene068488:intron 45.0%
! AGTGCTGCTAGTTACCTCAA+AGG + chr4.2:58316781-58316800 None:intergenic 45.0%
! TCAACAGAGGGAGTACATGT+GGG - chr4.2:58315929-58315948 MS.gene068488:intron 45.0%
! TGAAGGGATCTAGAACAGGA+TGG - chr4.2:58316688-58316707 MS.gene068488:intron 45.0%
! TTCAACAGAGGGAGTACATG+TGG - chr4.2:58315928-58315947 MS.gene068488:intron 45.0%
! TTTGAGGTAACTAGCAGCAC+TGG - chr4.2:58316780-58316799 MS.gene068488:CDS 45.0%
ACCAACTGAGCTAAGCTCAC+GGG + chr4.2:58315736-58315755 None:intergenic 50.0%
CACCAACTGAGCTAAGCTCA+CGG + chr4.2:58315737-58315756 None:intergenic 50.0%
GCACCTCCAAACTGGCAATT+TGG - chr4.2:58316828-58316847 MS.gene068488:CDS 50.0%
GCAGAAGGAGCTTGTGGTTA+TGG - chr4.2:58315850-58315869 MS.gene068488:intron 50.0%
GGCTACCAATGGCAAGGAAT+TGG - chr4.2:58316709-58316728 MS.gene068488:intron 50.0%
TGTGATGCAGAAGGAGCTTG+TGG - chr4.2:58315844-58315863 MS.gene068488:intron 50.0%
! CAACAGAGGGAGTACATGTG+GGG - chr4.2:58315930-58315949 MS.gene068488:intron 50.0%
! TGAGCTTAGCTCAGTTGGTG+TGG - chr4.2:58315737-58315756 MS.gene068488:CDS 50.0%
CAGGATGGCTACCAATGGCA+AGG - chr4.2:58316703-58316722 MS.gene068488:intron 55.0%
CCAACTGAGCTAAGCTCACG+GGG + chr4.2:58315735-58315754 None:intergenic 55.0%
CCCCGTGAGCTTAGCTCAGT+TGG - chr4.2:58315732-58315751 MS.gene068488:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 58315508 58316878 58315508 ID=MS.gene068488
chr4.2 mRNA 58315508 58316878 58315508 ID=MS.gene068488.t1;Parent=MS.gene068488
chr4.2 exon 58316731 58316878 58316731 ID=MS.gene068488.t1.exon1;Parent=MS.gene068488.t1
chr4.2 CDS 58316731 58316878 58316731 ID=cds.MS.gene068488.t1;Parent=MS.gene068488.t1
chr4.2 exon 58316214 58316532 58316214 ID=MS.gene068488.t1.exon2;Parent=MS.gene068488.t1
chr4.2 CDS 58316214 58316532 58316214 ID=cds.MS.gene068488.t1;Parent=MS.gene068488.t1
chr4.2 exon 58315508 58315811 58315508 ID=MS.gene068488.t1.exon3;Parent=MS.gene068488.t1
chr4.2 CDS 58315508 58315811 58315508 ID=cds.MS.gene068488.t1;Parent=MS.gene068488.t1
Gene Sequence

>MS.gene068488

ATGCTGTCATCTAAGATGGGTGTTCTTCAGTCCACACTTCTCTACTTGATTTTATTACATTCATTCAACATTGTTGCTTACAAAGATCAAGAATGGAAGAAAGCCACTGCAACTTATGCCAAAGACACAGAAGGATCTCTAATTACAGGTACTTTTCTTGCTCCTCCTTTGGTTCATAACAGCAACATTAATAGTCTTGGTTTGTCAAATGCACATGATTGTGTCCCCGTGAGCTTAGCTCAGTTGGTGTGGACAATGCATAATATATGCAAGATCTAGGGTTCAAACCCCGACCACCACCAAAAAAAAAAAAAATGTACATGATTTTTGTGTGCATGTGATGCAGAAGGAGCTTGTGGTTATGGAGATCTTCACAAAGCTAGCTATGGAAAACATAGTGTTGGATTAAGCACAATTTTGTTCAACAGAGGGAGTACATGTGGGGCTTGCTATGAGATCAGATGTGTTGACCATATCTTGTGGTGTATGCTTGGAAGTCCTTCTGTTATTGTTACTGCCACTGATTTCTGTCCTCCTAATTTTGGTCTCTCAGTTGATTATGGTGGTTGGTGTAACTTTCCAAGAGAACATTTTGAGTTGTCACTGGCTGCATTTGCTGAAATTGCTAAGGGGAAAGCTGATATTATTCCCGTTCAATATAGAAGGTAGATAGATTTTTTTATAAGTGTTTTTGGGATGTTTTTGGATAAACAACTTAATTAATCTCTTATTTGGTTAATGATGTTTATATAAGCGCTTATGTATAAACAATTTTTATAATCATAGAATACTGTCCTTGAACTGTTTTTATATAAACGTTTGGCTCAATTACACATTTGGTTTTTAACTTTCATTTTAGTTCCTTGTGTTTTTTAGTTTCATTTTGGTTGTAATTTAAGAGACTAATCTTAAATTGTAATTTAATTTATCCTAATCTTTTTTGCCTCTTTTTATTAGCTATGTTATAGAGCTTATTAAAATAAGTCAGAAAATAATTTATAAACATGTTATAAGTTTTCTTATAAACTCTTCCAAATTTTTGTTTTGTTTGTGCAAATGTGATGCAGAGTGAAGTGTGAAAGAAGTGGAGGGTTGAAATTCACAATGAGTGGAAGTTCTCATTTCTATCAAGTTCTGATAACTAATGTGGGGTTAGATGGTGAAGTTTTTGCTGTGAAAGTGAAGGGATCTAGAACAGGATGGCTACCAATGGCAAGGAATTGGGGAATGAATTGGCATTGCAATGTCAACCTTCAACATCAGCCTTTGTCCTTTGAGGTAACTAGCAGCACTGGAAAAACACTCACATCTTACAATGTAGCACCTCCAAACTGGCAATTTGGACAAACATTTGAAGGAAAACAATTTTAG
Protein sequence

>MS.gene068488.t1

MLSSKMGVLQSTLLYLILLHSFNIVAYKDQEWKKATATYAKDTEGSLITEGACGYGDLHKASYGKHSVGLSTILFNRGSTCGACYEIRCVDHILWCMLGSPSVIVTATDFCPPNFGLSVDYGGWCNFPREHFELSLAAFAEIAKGKADIIPVQYRRVKCERSGGLKFTMSGSSHFYQVLITNVGLDGEVFAVKVKGSRTGWLPMARNWGMNWHCNVNLQHQPLSFEVTSSTGKTLTSYNVAPPNWQFGQTFEGKQF