AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50162

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50162.t1 MTR_5g079950 98.456 259 4 0 1 259 1 259 0.0 527
MS.gene50162.t1 MTR_2g097030 83.398 259 41 1 1 259 4 260 6.18e-167 462
MS.gene50162.t1 MTR_7g061720 82.239 259 45 1 1 259 1 258 3.47e-163 452
MS.gene50162.t1 MTR_4g063975 81.349 252 43 1 12 259 7 258 5.84e-151 421
MS.gene50162.t1 MTR_3g094060 73.016 252 61 2 6 257 9 253 1.13e-134 380
MS.gene50162.t1 MTR_3g094040 72.619 252 62 2 6 257 9 253 2.60e-133 377
MS.gene50162.t1 MTR_5g041700 74.672 229 56 1 30 258 22 248 2.17e-128 364
MS.gene50162.t1 MTR_4g081950 71.138 246 68 2 10 254 37 280 2.96e-128 365
MS.gene50162.t1 MTR_5g075320 68.273 249 75 3 11 257 5 251 1.01e-123 352
MS.gene50162.t1 MTR_3g466790 68.110 254 78 2 5 257 3 254 8.07e-123 350
MS.gene50162.t1 MTR_3g435430 74.222 225 56 1 33 257 25 247 1.32e-122 349
MS.gene50162.t1 MTR_3g466760 66.926 257 82 2 1 257 7 260 1.90e-122 349
MS.gene50162.t1 MTR_7g011060 69.469 226 59 2 32 257 25 240 6.09e-116 332
MS.gene50162.t1 MTR_1g085460 65.289 242 76 4 17 255 18 254 5.70e-111 320
MS.gene50162.t1 MTR_1g026020 64.502 231 79 2 27 255 23 252 3.83e-109 315
MS.gene50162.t1 MTR_7g111010 60.000 255 92 5 1 252 1 248 7.95e-107 310
MS.gene50162.t1 MTR_4g124340 58.091 241 98 2 17 256 45 283 3.40e-106 309
MS.gene50162.t1 MTR_7g110990 64.732 224 75 3 32 252 11 233 1.55e-104 303
MS.gene50162.t1 MTR_6g012520 64.777 247 76 3 12 257 6 242 6.56e-103 299
MS.gene50162.t1 MTR_7g013150 56.000 250 101 2 6 255 10 250 1.53e-100 293
MS.gene50162.t1 MTR_8g464350 56.846 241 99 2 12 252 13 248 7.25e-99 289
MS.gene50162.t1 MTR_7g013120 55.469 256 107 4 1 255 1 250 8.00e-99 289
MS.gene50162.t1 MTR_8g058990 55.967 243 102 2 12 254 12 249 1.34e-98 288
MS.gene50162.t1 MTR_7g013140 55.510 245 102 2 10 254 10 247 8.85e-97 284
MS.gene50162.t1 MTR_5g011210 52.869 244 113 2 13 255 17 259 5.02e-94 277
MS.gene50162.t1 MTR_4g102450 53.725 255 113 4 8 258 9 262 8.57e-94 277
MS.gene50162.t1 MTR_5g076950 56.444 225 97 1 33 257 41 264 1.14e-91 271
MS.gene50162.t1 MTR_0040s0140 49.398 249 124 1 9 257 10 256 2.34e-88 263
MS.gene50162.t1 MTR_2g090255 46.825 252 131 1 6 257 4 252 1.59e-85 255
MS.gene50162.t1 MTR_3g064510 52.991 234 101 5 33 258 53 285 7.86e-80 242
MS.gene50162.t1 MTR_0008s0120 42.449 245 80 3 10 254 12 195 5.52e-58 183
MS.gene50162.t1 MTR_6g012500 54.167 120 49 1 144 257 161 280 3.93e-39 137
MS.gene50162.t1 MTR_2g090270 28.253 269 165 12 1 255 1 255 2.40e-24 98.6
MS.gene50162.t1 MTR_0122s0020 32.766 235 132 11 33 255 41 261 1.27e-23 96.7
MS.gene50162.t1 MTR_2g090265 27.341 267 168 11 1 255 1 253 1.71e-22 93.6
MS.gene50162.t1 MTR_4g028640 29.661 236 131 11 33 252 42 258 4.22e-20 87.0
MS.gene50162.t1 MTR_0118s0070 30.303 231 131 13 38 252 53 269 2.50e-19 85.1
MS.gene50162.t1 MTR_0809s0010 28.814 236 134 12 33 252 47 264 3.99e-19 84.7
MS.gene50162.t1 MTR_1g085365 62.500 56 21 0 202 257 4 59 1.36e-15 69.7
MS.gene50162.t1 MTR_4g075790 27.556 225 128 10 44 252 32 237 7.16e-14 69.7
MS.gene50162.t1 MTR_0122s0020 33.714 175 91 10 33 196 41 201 2.88e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50162.t1 AT2G39700 83.594 256 41 1 4 259 3 257 2.00e-166 461
MS.gene50162.t1 AT3G55500 81.853 259 46 1 1 259 3 260 2.12e-161 448
MS.gene50162.t1 AT2G37640 83.333 246 41 0 14 259 17 262 1.95e-159 443
MS.gene50162.t1 AT2G28950 83.665 251 41 0 9 259 7 257 8.90e-158 439
MS.gene50162.t1 AT5G02260 78.740 254 51 1 9 259 5 258 4.23e-155 432
MS.gene50162.t1 AT2G37640 84.286 210 33 0 50 259 28 237 1.12e-136 384
MS.gene50162.t1 AT1G69530 68.800 250 76 1 9 258 3 250 3.08e-125 356
MS.gene50162.t1 AT1G69530 68.800 250 76 1 9 258 3 250 3.08e-125 356
MS.gene50162.t1 AT1G69530 69.880 249 71 3 10 258 6 250 5.97e-124 353
MS.gene50162.t1 AT2G03090 66.409 259 78 3 1 258 3 253 1.19e-123 352
MS.gene50162.t1 AT5G56320 67.611 247 76 2 13 259 11 253 6.88e-123 350
MS.gene50162.t1 AT5G56320 67.611 247 76 2 13 259 11 253 6.88e-123 350
MS.gene50162.t1 AT5G05290 73.246 228 58 2 31 257 30 255 7.67e-123 350
MS.gene50162.t1 AT5G56320 67.611 247 76 2 13 259 11 253 6.31e-122 350
MS.gene50162.t1 AT2G40610 66.406 256 75 3 3 257 8 253 7.98e-120 342
MS.gene50162.t1 AT1G26770 70.040 247 71 2 12 258 6 249 3.25e-119 341
MS.gene50162.t1 AT1G26770 70.040 247 71 2 12 258 16 259 3.31e-119 341
MS.gene50162.t1 AT4G01630 62.000 250 91 3 6 252 1 249 6.36e-111 320
MS.gene50162.t1 AT3G29030 67.699 226 64 2 33 258 38 254 5.25e-110 318
MS.gene50162.t1 AT1G69530 62.400 250 62 2 9 258 3 220 2.93e-108 312
MS.gene50162.t1 AT1G69530 63.454 249 57 4 10 258 6 220 2.85e-107 310
MS.gene50162.t1 AT1G20190 63.470 219 77 2 37 253 30 247 7.46e-101 294
MS.gene50162.t1 AT5G39270 59.193 223 87 2 33 255 58 276 1.94e-98 289
MS.gene50162.t1 AT5G39290 58.744 223 88 2 33 255 58 276 6.72e-98 288
MS.gene50162.t1 AT1G62980 55.645 248 103 3 9 255 12 253 3.31e-96 283
MS.gene50162.t1 AT5G39280 56.696 224 93 2 32 255 53 272 4.49e-94 278
MS.gene50162.t1 AT5G39300 56.250 224 94 2 32 255 54 273 2.21e-93 276
MS.gene50162.t1 AT1G12560 53.175 252 114 4 9 258 13 262 3.08e-93 275
MS.gene50162.t1 AT3G03220 54.425 226 102 1 33 258 40 264 2.70e-88 263
MS.gene50162.t1 AT5G39310 54.018 224 98 3 33 255 90 309 2.29e-87 262
MS.gene50162.t1 AT1G20190 59.574 188 73 2 68 253 2 188 8.95e-80 239
MS.gene50162.t1 AT5G39260 50.394 254 111 5 6 255 17 259 4.91e-79 239
MS.gene50162.t1 AT3G15370 48.047 256 120 7 4 255 1 247 1.90e-78 237
MS.gene50162.t1 AT4G38210 46.371 248 131 1 13 258 8 255 9.22e-78 236
MS.gene50162.t1 AT3G15370 43.369 279 117 7 11 255 6 277 4.85e-72 222
MS.gene50162.t1 AT2G20750 29.304 273 159 12 6 257 8 267 6.84e-24 97.4
MS.gene50162.t1 AT4G28250 28.839 267 161 12 6 257 8 260 5.22e-23 95.1
MS.gene50162.t1 AT1G65680 30.638 235 134 11 33 252 47 267 4.06e-22 92.8
MS.gene50162.t1 AT4G28250 27.757 263 158 11 6 255 8 251 5.68e-20 86.7
MS.gene50162.t1 AT2G45110 29.707 239 127 11 35 252 35 253 4.30e-19 84.3
MS.gene50162.t1 AT1G65681 28.767 219 129 9 47 252 12 216 1.76e-18 82.0
MS.gene50162.t1 AT4G17030 26.800 250 160 9 13 252 7 243 2.65e-16 76.6
MS.gene50162.t1 AT3G60570 27.068 266 163 10 10 259 9 259 5.47e-16 75.9
MS.gene50162.t1 AT3G45970 25.352 213 137 9 51 252 14 215 6.09e-15 72.4
MS.gene50162.t1 AT2G20750 27.536 207 123 7 6 196 8 203 3.38e-14 70.1
MS.gene50162.t1 AT4G28250 28.780 205 116 9 6 199 8 193 1.97e-13 67.8
MS.gene50162.t1 AT4G28250 29.814 161 90 6 6 157 8 154 4.02e-13 67.0
MS.gene50162.t1 AT3G45970 24.519 208 135 9 56 252 46 242 2.79e-12 65.5
MS.gene50162.t1 AT3G45960 26.316 209 129 11 56 252 46 241 1.05e-11 63.9

Find 75 sgRNAs with CRISPR-Local

Find 123 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAATATTGTTCCCTCTAAT+TGG 0.218483 5.4:-62820396 MS.gene50162:CDS
TCACTTGGTTGAGCAAAATT+TGG 0.226173 5.4:+62821530 None:intergenic
GTTCCCTCTAATTGGCAATT+TGG 0.258104 5.4:-62820388 MS.gene50162:CDS
TTGGCAATCAAATTCTGTTT+TGG 0.280999 5.4:-62820476 MS.gene50162:CDS
ATAAACGTACTTCAACTTCT+TGG 0.285149 5.4:-62820417 MS.gene50162:CDS
ACCAATATTGAAGGACTTCC+AGG 0.314188 5.4:+62821575 None:intergenic
TTTGCTCAACCAAGTGATAA+TGG 0.320638 5.4:-62821524 MS.gene50162:CDS
CCCTGGAAGTCCTTCAATAT+TGG 0.339675 5.4:-62821576 MS.gene50162:CDS
GGATGAGTATGAGTCGTAAC+TGG 0.344835 5.4:-62820507 MS.gene50162:CDS
TTGACCAAATTGCCAATTAG+AGG 0.346926 5.4:+62820384 None:intergenic
TTAGGTGGAGCATGTGGATA+TGG 0.347554 5.4:-62821722 MS.gene50162:intron
GTTCTAATCACCAACGTTGC+AGG 0.349710 5.4:-62820577 MS.gene50162:CDS
ATAGTGAAGACTAGTGTTAA+AGG 0.372536 5.4:-62820544 MS.gene50162:CDS
AAGGGCTAAGAAGGTGAAAA+TGG 0.373363 5.4:+62822020 None:intergenic
GGCGTCGCTGCCGCCGTAGA+AGG 0.408061 5.4:+62821927 None:intergenic
GGTGTAGACACCGGGGAGTC+TGG 0.415380 5.4:+62821978 None:intergenic
AGTGAGGAGAAGGGCTAAGA+AGG 0.427838 5.4:+62822011 None:intergenic
CAATTTGGTCAAACTTTCAC+TGG 0.428540 5.4:-62820373 MS.gene50162:CDS
AATTTGTATAGTCAAGGTTA+TGG 0.433320 5.4:-62821698 MS.gene50162:CDS
GTAGCAGTTACCAATATTGA+AGG 0.440044 5.4:+62821566 None:intergenic
AGTTACCTGCGGTAAGAAAC+AGG 0.447956 5.4:+62821416 None:intergenic
CACCATCCTCCATTATCACT+TGG 0.454289 5.4:+62821515 None:intergenic
ACCGCTCACGCCACCTTCTA+CGG 0.458135 5.4:-62821940 MS.gene50162:CDS
CGGCTTCCGTTACTTCAACT+TGG 0.462141 5.4:-62820599 MS.gene50162:CDS
GACACCGGGGAGTCTGGCTT+CGG 0.467921 5.4:+62821984 None:intergenic
ATCACCAACGTTGCAGGTGC+AGG 0.472734 5.4:-62820571 MS.gene50162:CDS
GTGGCACCAACTAGGATCAT+TGG 0.475998 5.4:+62821598 None:intergenic
CTTGGTTGAGCAAAATTTGG+TGG 0.486783 5.4:+62821533 None:intergenic
ATCACCTGCACCTGCAACGT+TGG 0.490975 5.4:+62820567 None:intergenic
AGCGGTTTGCCATGCTCCGC+CGG 0.491165 5.4:+62821957 None:intergenic
TACTAACTAACCCATTGTTC+CGG 0.491667 5.4:+62821903 None:intergenic
AGTGTTCCAATGATCCTAGT+TGG 0.492135 5.4:-62821604 MS.gene50162:CDS
TACGGCGGCAGCGACGCCTC+CGG 0.495150 5.4:-62821922 MS.gene50162:CDS
AGTTGAAGTACGTTTATCAC+TGG 0.500062 5.4:+62820423 None:intergenic
CAGCGACGCCTCCGGAACAA+TGG 0.502237 5.4:-62821914 MS.gene50162:CDS
AGCGACGCCTCCGGAACAAT+GGG 0.505728 5.4:-62821913 MS.gene50162:intron
TTGTTCCTGTTTCTTACCGC+AGG 0.518212 5.4:-62821421 MS.gene50162:intron
CTTCGGTTGAAGTGAGGAGA+AGG 0.527312 5.4:+62822001 None:intergenic
TTCAACAACGGTTTAAGTTG+TGG 0.528411 5.4:-62821644 MS.gene50162:CDS
GCTCCGCCGGTGTAGACACC+GGG 0.534852 5.4:+62821970 None:intergenic
AAAATTGCTCAATATCGTGC+TGG 0.536520 5.4:-62821446 MS.gene50162:CDS
TGCTCCGCCGGTGTAGACAC+CGG 0.538847 5.4:+62821969 None:intergenic
GCAAGATCAAAGTGTGGTCG+TGG 0.539841 5.4:+62821485 None:intergenic
TGACCAAATTGCCAATTAGA+GGG 0.540758 5.4:+62820385 None:intergenic
TATGGAAATTTGTATAGTCA+AGG 0.544667 5.4:-62821704 MS.gene50162:CDS
CTTCCAGGGTGGCACCAACT+AGG 0.546110 5.4:+62821590 None:intergenic
GTCGCTGCCGCCGTAGAAGG+TGG 0.555378 5.4:+62821930 None:intergenic
GATGAGTATGAGTCGTAACT+GGG 0.556251 5.4:-62820506 MS.gene50162:CDS
GCCGTAGAAGGTGGCGTGAG+CGG 0.563764 5.4:+62821939 None:intergenic
GATCCTAGTTGGTGCCACCC+TGG 0.564618 5.4:-62821593 MS.gene50162:CDS
GTGTCTACACCGGCGGAGCA+TGG 0.565072 5.4:-62821966 MS.gene50162:CDS
GGAATAAGATACACAATCAA+CGG 0.582244 5.4:-62820619 MS.gene50162:CDS
AGGTGAAAATGGTGGTGATG+TGG 0.582302 5.4:+62822031 None:intergenic
TCAACCGAAGCCAGACTCCC+CGG 0.594710 5.4:-62821988 MS.gene50162:CDS
GATCAAAGTGTGGTCGTGGA+GGG 0.601508 5.4:+62821489 None:intergenic
AGGGTTCCATGCAGAAAACA+AGG 0.610579 5.4:-62820643 MS.gene50162:CDS
TTAGAACCAAGTTGAAGTAA+CGG 0.614961 5.4:+62820593 None:intergenic
AGATCAAAGTGTGGTCGTGG+AGG 0.616640 5.4:+62821488 None:intergenic
GTCTGGCTTCGGTTGAAGTG+AGG 0.619944 5.4:+62821995 None:intergenic
CCAATATTGAAGGACTTCCA+GGG 0.631547 5.4:+62821576 None:intergenic
GCTCACGCCACCTTCTACGG+CGG 0.631792 5.4:-62821937 MS.gene50162:CDS
TTCGGTTGAAGTGAGGAGAA+GGG 0.636772 5.4:+62822002 None:intergenic
AGACTCCCCGGTGTCTACAC+CGG 0.637890 5.4:-62821976 MS.gene50162:CDS
AACCAAGTGATAATGGAGGA+TGG 0.639235 5.4:-62821517 MS.gene50162:CDS
AGCACAGCACTATTCAACAA+CGG 0.641968 5.4:-62821656 MS.gene50162:CDS
GGCTAAGAAGGTGAAAATGG+TGG 0.650038 5.4:+62822023 None:intergenic
CTCCCCGGTGTCTACACCGG+CGG 0.653439 5.4:-62821973 MS.gene50162:CDS
TAACTAACCCATTGTTCCGG+AGG 0.665327 5.4:+62821906 None:intergenic
GCTCAACCAAGTGATAATGG+AGG 0.668747 5.4:-62821521 MS.gene50162:CDS
TGAGTATGAGTCGTAACTGG+GGG 0.680229 5.4:-62820504 MS.gene50162:CDS
ATGAGTATGAGTCGTAACTG+GGG 0.683363 5.4:-62820505 MS.gene50162:CDS
GGCATAGCAAGATCAAAGTG+TGG 0.696702 5.4:+62821479 None:intergenic
CTCCGCCGGTGTAGACACCG+GGG 0.749196 5.4:+62821971 None:intergenic
GTTCCATGCAGAAAACAAGG+AGG 0.759316 5.4:-62820640 MS.gene50162:CDS
ATATTGAAGGACTTCCAGGG+TGG 0.792160 5.4:+62821579 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAAAGAAAATGAAATT+TGG + chr5.4:62821028-62821047 None:intergenic 10.0%
!! ATAAAAAATAAAGAAAATTG+CGG + chr5.4:62820545-62820564 None:intergenic 10.0%
!!! GAAAATATAATATTTTTACA+AGG + chr5.4:62821117-62821136 None:intergenic 10.0%
!!! TTTTATAATTAGTACTATTA+TGG - chr5.4:62821179-62821198 MS.gene50162:intron 10.0%
!!! TTTTTTGTTATATTTGTAAA+TGG + chr5.4:62821339-62821358 None:intergenic 10.0%
!! AAAATAAAAAGAAACTAAAG+AGG + chr5.4:62821163-62821182 None:intergenic 15.0%
!! ATAATAAACACAAACAAATT+AGG + chr5.4:62821059-62821078 None:intergenic 15.0%
!!! ATGGTCTTATTTTTTTTTAA+AGG + chr5.4:62821320-62821339 None:intergenic 15.0%
!!! TTAAATTTTGGTTACATTTT+AGG - chr5.4:62820656-62820675 MS.gene50162:intron 15.0%
!! ATGGGAAAAAAATGTAAAAA+AGG + chr5.4:62821139-62821158 None:intergenic 20.0%
!! GTAATAAAACACAACATAAA+GGG + chr5.4:62821686-62821705 None:intergenic 20.0%
!! TCAATATTGTTAGATTGAAA+TGG - chr5.4:62821520-62821539 MS.gene50162:CDS 20.0%
!! TGTAATAAAACACAACATAA+AGG + chr5.4:62821687-62821706 None:intergenic 20.0%
!!! CACTTCATGAAATTTTTATA+TGG + chr5.4:62821231-62821250 None:intergenic 20.0%
!!! TTTGTGCTAATGTTAAATTT+TGG - chr5.4:62820644-62820663 MS.gene50162:CDS 20.0%
! AATTTGTATAGTCAAGGTTA+TGG - chr5.4:62820698-62820717 MS.gene50162:intron 25.0%
! TATGGAAATTTGTATAGTCA+AGG - chr5.4:62820692-62820711 MS.gene50162:intron 25.0%
!!! AATTTTGGTTACATTTTAGG+TGG - chr5.4:62820659-62820678 MS.gene50162:intron 25.0%
AAAAAGAAACTAAAGAGGCA+TGG + chr5.4:62821158-62821177 None:intergenic 30.0%
AAATAAAGAAAATTGCGGCA+TGG + chr5.4:62820540-62820559 None:intergenic 30.0%
AGTGTTAAAGGTTCAAAAAC+TGG - chr5.4:62821864-62821883 MS.gene50162:intron 30.0%
ATAAACGTACTTCAACTTCT+TGG - chr5.4:62821979-62821998 MS.gene50162:CDS 30.0%
ATAGTGAAGACTAGTGTTAA+AGG - chr5.4:62821852-62821871 MS.gene50162:intron 30.0%
ATTTCATGAAGTGTGATTGA+AGG - chr5.4:62821238-62821257 MS.gene50162:intron 30.0%
GGAATAAGATACACAATCAA+CGG - chr5.4:62821777-62821796 MS.gene50162:intron 30.0%
TTAAAGGTTCAAAAACTGGT+TGG - chr5.4:62821868-62821887 MS.gene50162:intron 30.0%
TTGAAATGGTTGATCTAAAC+CGG - chr5.4:62821534-62821553 MS.gene50162:CDS 30.0%
! AAAAGAAACTAAAGAGGCAT+GGG + chr5.4:62821157-62821176 None:intergenic 30.0%
! TGGTCAATCACTTTCTTTTA+GGG - chr5.4:62821944-62821963 MS.gene50162:CDS 30.0%
! TTAGAACCAAGTTGAAGTAA+CGG + chr5.4:62821806-62821825 None:intergenic 30.0%
! TTGGCAATCAAATTCTGTTT+TGG - chr5.4:62821920-62821939 MS.gene50162:CDS 30.0%
! TTGGTCAATCACTTTCTTTT+AGG - chr5.4:62821943-62821962 MS.gene50162:CDS 30.0%
!!! CAATCAAATTCTGTTTTGGT+TGG - chr5.4:62821924-62821943 MS.gene50162:CDS 30.0%
AAAATTGCTCAATATCGTGC+TGG - chr5.4:62820950-62820969 MS.gene50162:intron 35.0%
ACTAAAGACATCATGTACGA+AGG - chr5.4:62821412-62821431 MS.gene50162:intron 35.0%
AGTTGAAGTACGTTTATCAC+TGG + chr5.4:62821976-62821995 None:intergenic 35.0%
CAATTTGGTCAAACTTTCAC+TGG - chr5.4:62822023-62822042 MS.gene50162:CDS 35.0%
GGAATATTGTTCCCTCTAAT+TGG - chr5.4:62822000-62822019 MS.gene50162:CDS 35.0%
GTACTAGTATAGTACAAGAG+TGG + chr5.4:62821642-62821661 None:intergenic 35.0%
GTAGCAGTTACCAATATTGA+AGG + chr5.4:62820833-62820852 None:intergenic 35.0%
TAAATATGCGGCAAAATGAC+TGG + chr5.4:62821578-62821597 None:intergenic 35.0%
TACTAACTAACCCATTGTTC+CGG + chr5.4:62820496-62820515 None:intergenic 35.0%
TCACTTGGTTGAGCAAAATT+TGG + chr5.4:62820869-62820888 None:intergenic 35.0%
TCGTGAGTAGTGTAAATATG+CGG + chr5.4:62821590-62821609 None:intergenic 35.0%
TGACCAAATTGCCAATTAGA+GGG + chr5.4:62822014-62822033 None:intergenic 35.0%
TGTCCTTTGATATGCATGTA+GGG - chr5.4:62821734-62821753 MS.gene50162:intron 35.0%
TTGACCAAATTGCCAATTAG+AGG + chr5.4:62822015-62822034 None:intergenic 35.0%
TTTGCTCAACCAAGTGATAA+TGG - chr5.4:62820872-62820891 MS.gene50162:intron 35.0%
! CACGATATTGAGCAATTTTG+AGG + chr5.4:62820949-62820968 None:intergenic 35.0%
!! TGAGCAATTTTGAGGAACAT+AGG + chr5.4:62820941-62820960 None:intergenic 35.0%
!! TTCAACAACGGTTTAAGTTG+TGG - chr5.4:62820752-62820771 MS.gene50162:intron 35.0%
AAAGAAAATTGCGGCATGGT+TGG + chr5.4:62820536-62820555 None:intergenic 40.0%
AAGGGCTAAGAAGGTGAAAA+TGG + chr5.4:62820379-62820398 None:intergenic 40.0%
ACAAGACAAGTAGTTACCTG+CGG + chr5.4:62820994-62821013 None:intergenic 40.0%
ACCAATATTGAAGGACTTCC+AGG + chr5.4:62820824-62820843 None:intergenic 40.0%
AGCACAGCACTATTCAACAA+CGG - chr5.4:62820740-62820759 MS.gene50162:intron 40.0%
AGTGTTCCAATGATCCTAGT+TGG - chr5.4:62820792-62820811 MS.gene50162:intron 40.0%
ATCAACTGAGCTACGTTTAC+GGG + chr5.4:62821389-62821408 None:intergenic 40.0%
ATGAGTATGAGTCGTAACTG+GGG - chr5.4:62821891-62821910 MS.gene50162:intron 40.0%
CATCAACTGAGCTACGTTTA+CGG + chr5.4:62821390-62821409 None:intergenic 40.0%
CCAATATTGAAGGACTTCCA+GGG + chr5.4:62820823-62820842 None:intergenic 40.0%
CTTGGTTGAGCAAAATTTGG+TGG + chr5.4:62820866-62820885 None:intergenic 40.0%
GAACCCTACATGCATATCAA+AGG + chr5.4:62821740-62821759 None:intergenic 40.0%
GATGAGTATGAGTCGTAACT+GGG - chr5.4:62821890-62821909 MS.gene50162:intron 40.0%
GTGTCCTTTGATATGCATGT+AGG - chr5.4:62821733-62821752 MS.gene50162:intron 40.0%
GTTCCCTCTAATTGGCAATT+TGG - chr5.4:62822008-62822027 MS.gene50162:CDS 40.0%
GTTTGTCACGAAACTTGAAC+CGG + chr5.4:62821556-62821575 None:intergenic 40.0%
! GACACGACTATTTTCAGCAA+GGG + chr5.4:62821718-62821737 None:intergenic 40.0%
!! AACCAAGTGATAATGGAGGA+TGG - chr5.4:62820879-62820898 MS.gene50162:intron 40.0%
!! TACATTTTAGGTGGAGCATG+TGG - chr5.4:62820668-62820687 MS.gene50162:intron 40.0%
!!! ATTCCTCCTTGTTTTCTGCA+TGG + chr5.4:62821762-62821781 None:intergenic 40.0%
AGGGTTCCATGCAGAAAACA+AGG - chr5.4:62821753-62821772 MS.gene50162:intron 45.0%
AGTTACCTGCGGTAAGAAAC+AGG + chr5.4:62820983-62821002 None:intergenic 45.0%
ATATTGAAGGACTTCCAGGG+TGG + chr5.4:62820820-62820839 None:intergenic 45.0%
CACCATCCTCCATTATCACT+TGG + chr5.4:62820884-62820903 None:intergenic 45.0%
CCCTGGAAGTCCTTCAATAT+TGG - chr5.4:62820820-62820839 MS.gene50162:intron 45.0%
GGATGAGTATGAGTCGTAAC+TGG - chr5.4:62821889-62821908 MS.gene50162:intron 45.0%
GGCATAGCAAGATCAAAGTG+TGG + chr5.4:62820920-62820939 None:intergenic 45.0%
GGCTAAGAAGGTGAAAATGG+TGG + chr5.4:62820376-62820395 None:intergenic 45.0%
GTTCCATGCAGAAAACAAGG+AGG - chr5.4:62821756-62821775 MS.gene50162:intron 45.0%
GTTCTAATCACCAACGTTGC+AGG - chr5.4:62821819-62821838 MS.gene50162:intron 45.0%
TAACTAACCCATTGTTCCGG+AGG + chr5.4:62820493-62820512 None:intergenic 45.0%
TGAGTATGAGTCGTAACTGG+GGG - chr5.4:62821892-62821911 MS.gene50162:intron 45.0%
TTAGGTGGAGCATGTGGATA+TGG - chr5.4:62820674-62820693 MS.gene50162:intron 45.0%
TTCGGTTGAAGTGAGGAGAA+GGG + chr5.4:62820397-62820416 None:intergenic 45.0%
! AGGTGAAAATGGTGGTGATG+TGG + chr5.4:62820368-62820387 None:intergenic 45.0%
! GGACACGACTATTTTCAGCA+AGG + chr5.4:62821719-62821738 None:intergenic 45.0%
! TTGTTCCTGTTTCTTACCGC+AGG - chr5.4:62820975-62820994 MS.gene50162:intron 45.0%
!! GCTCAACCAAGTGATAATGG+AGG - chr5.4:62820875-62820894 MS.gene50162:intron 45.0%
!! ATATTAAAAATTACTATAAT+AGG - chr5.4:62821443-62821462 MS.gene50162:CDS 5.0%
AGATCAAAGTGTGGTCGTGG+AGG + chr5.4:62820911-62820930 None:intergenic 50.0%
AGTGAGGAGAAGGGCTAAGA+AGG + chr5.4:62820388-62820407 None:intergenic 50.0%
CGGCTTCCGTTACTTCAACT+TGG - chr5.4:62821797-62821816 MS.gene50162:intron 50.0%
CTTCGGTTGAAGTGAGGAGA+AGG + chr5.4:62820398-62820417 None:intergenic 50.0%
GAAAATTGCGGCATGGTTGG+TGG + chr5.4:62820533-62820552 None:intergenic 50.0%
GACGCGAGTTTGAATCTGCT+AGG + chr5.4:62820580-62820599 None:intergenic 50.0%
GATCAAAGTGTGGTCGTGGA+GGG + chr5.4:62820910-62820929 None:intergenic 50.0%
GCAAGATCAAAGTGTGGTCG+TGG + chr5.4:62820914-62820933 None:intergenic 50.0%
GTCGTAACTGGGGGCAAAAT+TGG - chr5.4:62821901-62821920 MS.gene50162:intron 50.0%
GTGGCACCAACTAGGATCAT+TGG + chr5.4:62820801-62820820 None:intergenic 50.0%
ATCACCAACGTTGCAGGTGC+AGG - chr5.4:62821825-62821844 MS.gene50162:intron 55.0%
ATCACCTGCACCTGCAACGT+TGG + chr5.4:62821832-62821851 None:intergenic 55.0%
! GTCTGGCTTCGGTTGAAGTG+AGG + chr5.4:62820404-62820423 None:intergenic 55.0%
ACCGCTCACGCCACCTTCTA+CGG - chr5.4:62820456-62820475 MS.gene50162:CDS 60.0%
AGACTCCCCGGTGTCTACAC+CGG - chr5.4:62820420-62820439 MS.gene50162:CDS 60.0%
AGCGACGCCTCCGGAACAAT+GGG - chr5.4:62820483-62820502 MS.gene50162:CDS 60.0%
CTTCCAGGGTGGCACCAACT+AGG + chr5.4:62820809-62820828 None:intergenic 60.0%
TCAACCGAAGCCAGACTCCC+CGG - chr5.4:62820408-62820427 MS.gene50162:CDS 60.0%
!! GATCCTAGTTGGTGCCACCC+TGG - chr5.4:62820803-62820822 MS.gene50162:intron 60.0%
AGCGGTTTGCCATGCTCCGC+CGG + chr5.4:62820442-62820461 None:intergenic 65.0%
CAGCGACGCCTCCGGAACAA+TGG - chr5.4:62820482-62820501 MS.gene50162:CDS 65.0%
GCCGTAGAAGGTGGCGTGAG+CGG + chr5.4:62820460-62820479 None:intergenic 65.0%
GCTCACGCCACCTTCTACGG+CGG - chr5.4:62820459-62820478 MS.gene50162:CDS 65.0%
GGTGTAGACACCGGGGAGTC+TGG + chr5.4:62820421-62820440 None:intergenic 65.0%
GTGTCTACACCGGCGGAGCA+TGG - chr5.4:62820430-62820449 MS.gene50162:CDS 65.0%
TGCTCCGCCGGTGTAGACAC+CGG + chr5.4:62820430-62820449 None:intergenic 65.0%
! GACACCGGGGAGTCTGGCTT+CGG + chr5.4:62820415-62820434 None:intergenic 65.0%
CTCCCCGGTGTCTACACCGG+CGG - chr5.4:62820423-62820442 MS.gene50162:CDS 70.0%
CTCCGCCGGTGTAGACACCG+GGG + chr5.4:62820428-62820447 None:intergenic 70.0%
GCTCCGCCGGTGTAGACACC+GGG + chr5.4:62820429-62820448 None:intergenic 70.0%
GTCGCTGCCGCCGTAGAAGG+TGG + chr5.4:62820469-62820488 None:intergenic 70.0%
GGCGTCGCTGCCGCCGTAGA+AGG + chr5.4:62820472-62820491 None:intergenic 75.0%
TACGGCGGCAGCGACGCCTC+CGG - chr5.4:62820474-62820493 MS.gene50162:CDS 75.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 62820354 62822064 62820354 ID=MS.gene50162
chr5.4 mRNA 62820354 62822064 62820354 ID=MS.gene50162.t1;Parent=MS.gene50162
chr5.4 exon 62821914 62822064 62821914 ID=MS.gene50162.t1.exon1;Parent=MS.gene50162.t1
chr5.4 CDS 62821914 62822064 62821914 ID=cds.MS.gene50162.t1;Parent=MS.gene50162.t1
chr5.4 exon 62821422 62821740 62821422 ID=MS.gene50162.t1.exon2;Parent=MS.gene50162.t1
chr5.4 CDS 62821422 62821740 62821422 ID=cds.MS.gene50162.t1;Parent=MS.gene50162.t1
chr5.4 exon 62820354 62820663 62820354 ID=MS.gene50162.t1.exon3;Parent=MS.gene50162.t1
chr5.4 CDS 62820354 62820663 62820354 ID=cds.MS.gene50162.t1;Parent=MS.gene50162.t1
Gene Sequence

>MS.gene50162

ATGACTAAGCTCCACATCACCACCATTTTCACCTTCTTAGCCCTTCTCCTCACTTCAACCGAAGCCAGACTCCCCGGTGTCTACACCGGCGGAGCATGGCAAACCGCTCACGCCACCTTCTACGGCGGCAGCGACGCCTCCGGAACAATGGGTTAGTTAGTACTAGAAAAAAATAACCACCAACCATGCCGCAATTTTCTTTATTTTTTATTCTAAAAATATACCTAGCAGATTCAAACTCGCGTCGCAACATATCAAATGTCTCTTATATATTCATTTAATAATGCTTTTTTGTGCTAATGTTAAATTTTGGTTACATTTTAGGTGGAGCATGTGGATATGGAAATTTGTATAGTCAAGGTTATGGTGTGAACACAGCAGCATTAAGCACAGCACTATTCAACAACGGTTTAAGTTGTGGCTCGTGTTTTGAGTTAAAGTGTTCCAATGATCCTAGTTGGTGCCACCCTGGAAGTCCTTCAATATTGGTAACTGCTACAAATTTTTGTCCACCAAATTTTGCTCAACCAAGTGATAATGGAGGATGGTGTAACCCTCCACGACCACACTTTGATCTTGCTATGCCTATGTTCCTCAAAATTGCTCAATATCGTGCTGGTATTGTTCCTGTTTCTTACCGCAGGTAACTACTTGTCTTGTTTACTTTCACTCCAAATTTCATTTTCTTTTTTATAAAACTAGCCTAATTTGTTTGTGTTTATTATTTTCTTTTATTTCATTGTTTTAATCTTGTAGCATTCCTTGTAAAAATATTATATTTTCCTTTTTTACATTTTTTTCCCATGCCTCTTTAGTTTCTTTTTATTTTATAATTAGTACTATTATGGAAGAAAAAAATTGTACAGTACTTTTTCCATATAAAAATTTCATGAAGTGTGATTGAAGGTATCTTTTTTAATTTTTTGTAAAGATGCTAAAAATTAATATCACTATATTTTGATTCCTTTAAAAAAAAATAAGACCATTTACAAATATAACAAAAAAAATCATTATAAAGAATAATGTAAATGTCCCGTAAACGTAGCTCAGTTGATGCTACTAAAGACATCATGTACGAAGGTACTATAAATATTAAAAATTACTATAATAGGATAAATCAAAATTAAAATGAAATCAATCAAAATTTTTAAGTCTATTTGATAATGTCAATATTGTTAGATTGAAATGGTTGATCTAAACCGGTTCAAGTTTCGTGACAAACCAGTCATTTTGCCGCATATTTACACTACTCACGAAACAACAAAATTTAGAATCTACTTTTCTGCCACTCTTGTACTATACTAGTACTAGTTAACATATATTTTATTGCCCTTTATGTTGTGTTTTATTACAACTTTATACCCTTGCTGAAAATAGTCGTGTCCTTTGATATGCATGTAGGGTTCCATGCAGAAAACAAGGAGGAATAAGATACACAATCAACGGCTTCCGTTACTTCAACTTGGTTCTAATCACCAACGTTGCAGGTGCAGGTGATATAGTGAAGACTAGTGTTAAAGGTTCAAAAACTGGTTGGATGAGTATGAGTCGTAACTGGGGGCAAAATTGGCAATCAAATTCTGTTTTGGTTGGTCAATCACTTTCTTTTAGGGTTACATCCAGTGATAAACGTACTTCAACTTCTTGGAATATTGTTCCCTCTAATTGGCAATTTGGTCAAACTTTCACTGGCAAGAATTTTCGAGTCTAA
Protein sequence

>MS.gene50162.t1

MTKLHITTIFTFLALLLTSTEARLPGVYTGGAWQTAHATFYGGSDASGTMGGACGYGNLYSQGYGVNTAALSTALFNNGLSCGSCFELKCSNDPSWCHPGSPSILVTATNFCPPNFAQPSDNGGWCNPPRPHFDLAMPMFLKIAQYRAGIVPVSYRRVPCRKQGGIRYTINGFRYFNLVLITNVAGAGDIVKTSVKGSKTGWMSMSRNWGQNWQSNSVLVGQSLSFRVTSSDKRTSTSWNIVPSNWQFGQTFTGKNFRV