AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene29490

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29490.t1 MTR_5g079950 98.456 259 4 0 1 259 1 259 0.0 527
MS.gene29490.t1 MTR_2g097030 83.398 259 41 1 1 259 4 260 6.18e-167 462
MS.gene29490.t1 MTR_7g061720 82.239 259 45 1 1 259 1 258 3.47e-163 452
MS.gene29490.t1 MTR_4g063975 81.349 252 43 1 12 259 7 258 5.84e-151 421
MS.gene29490.t1 MTR_3g094060 73.016 252 61 2 6 257 9 253 1.13e-134 380
MS.gene29490.t1 MTR_3g094040 72.619 252 62 2 6 257 9 253 2.60e-133 377
MS.gene29490.t1 MTR_5g041700 74.672 229 56 1 30 258 22 248 2.17e-128 364
MS.gene29490.t1 MTR_4g081950 71.138 246 68 2 10 254 37 280 2.96e-128 365
MS.gene29490.t1 MTR_5g075320 68.273 249 75 3 11 257 5 251 1.01e-123 352
MS.gene29490.t1 MTR_3g466790 68.110 254 78 2 5 257 3 254 8.07e-123 350
MS.gene29490.t1 MTR_3g435430 74.222 225 56 1 33 257 25 247 1.32e-122 349
MS.gene29490.t1 MTR_3g466760 66.926 257 82 2 1 257 7 260 1.90e-122 349
MS.gene29490.t1 MTR_7g011060 69.469 226 59 2 32 257 25 240 6.09e-116 332
MS.gene29490.t1 MTR_1g085460 65.289 242 76 4 17 255 18 254 5.70e-111 320
MS.gene29490.t1 MTR_1g026020 64.502 231 79 2 27 255 23 252 3.83e-109 315
MS.gene29490.t1 MTR_7g111010 60.000 255 92 5 1 252 1 248 7.95e-107 310
MS.gene29490.t1 MTR_4g124340 58.091 241 98 2 17 256 45 283 3.40e-106 309
MS.gene29490.t1 MTR_7g110990 64.732 224 75 3 32 252 11 233 1.55e-104 303
MS.gene29490.t1 MTR_6g012520 64.777 247 76 3 12 257 6 242 6.56e-103 299
MS.gene29490.t1 MTR_7g013150 56.000 250 101 2 6 255 10 250 1.53e-100 293
MS.gene29490.t1 MTR_8g464350 56.846 241 99 2 12 252 13 248 7.25e-99 289
MS.gene29490.t1 MTR_7g013120 55.469 256 107 4 1 255 1 250 8.00e-99 289
MS.gene29490.t1 MTR_8g058990 55.967 243 102 2 12 254 12 249 1.34e-98 288
MS.gene29490.t1 MTR_7g013140 55.510 245 102 2 10 254 10 247 8.85e-97 284
MS.gene29490.t1 MTR_5g011210 52.869 244 113 2 13 255 17 259 5.02e-94 277
MS.gene29490.t1 MTR_4g102450 53.725 255 113 4 8 258 9 262 8.57e-94 277
MS.gene29490.t1 MTR_5g076950 56.444 225 97 1 33 257 41 264 1.14e-91 271
MS.gene29490.t1 MTR_0040s0140 49.398 249 124 1 9 257 10 256 2.34e-88 263
MS.gene29490.t1 MTR_2g090255 46.825 252 131 1 6 257 4 252 1.59e-85 255
MS.gene29490.t1 MTR_3g064510 52.991 234 101 5 33 258 53 285 7.86e-80 242
MS.gene29490.t1 MTR_0008s0120 42.449 245 80 3 10 254 12 195 5.52e-58 183
MS.gene29490.t1 MTR_6g012500 54.167 120 49 1 144 257 161 280 3.93e-39 137
MS.gene29490.t1 MTR_2g090270 28.253 269 165 12 1 255 1 255 2.40e-24 98.6
MS.gene29490.t1 MTR_0122s0020 32.766 235 132 11 33 255 41 261 1.27e-23 96.7
MS.gene29490.t1 MTR_2g090265 27.341 267 168 11 1 255 1 253 1.71e-22 93.6
MS.gene29490.t1 MTR_4g028640 29.661 236 131 11 33 252 42 258 4.22e-20 87.0
MS.gene29490.t1 MTR_0118s0070 30.303 231 131 13 38 252 53 269 2.50e-19 85.1
MS.gene29490.t1 MTR_0809s0010 28.814 236 134 12 33 252 47 264 3.99e-19 84.7
MS.gene29490.t1 MTR_1g085365 62.500 56 21 0 202 257 4 59 1.36e-15 69.7
MS.gene29490.t1 MTR_4g075790 27.556 225 128 10 44 252 32 237 7.16e-14 69.7
MS.gene29490.t1 MTR_0122s0020 33.714 175 91 10 33 196 41 201 2.88e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29490.t1 AT2G39700 83.594 256 41 1 4 259 3 257 2.00e-166 461
MS.gene29490.t1 AT3G55500 81.853 259 46 1 1 259 3 260 2.12e-161 448
MS.gene29490.t1 AT2G37640 83.333 246 41 0 14 259 17 262 1.95e-159 443
MS.gene29490.t1 AT2G28950 83.665 251 41 0 9 259 7 257 8.90e-158 439
MS.gene29490.t1 AT5G02260 78.740 254 51 1 9 259 5 258 4.23e-155 432
MS.gene29490.t1 AT2G37640 84.286 210 33 0 50 259 28 237 1.12e-136 384
MS.gene29490.t1 AT1G69530 68.800 250 76 1 9 258 3 250 3.08e-125 356
MS.gene29490.t1 AT1G69530 68.800 250 76 1 9 258 3 250 3.08e-125 356
MS.gene29490.t1 AT1G69530 69.880 249 71 3 10 258 6 250 5.97e-124 353
MS.gene29490.t1 AT2G03090 66.409 259 78 3 1 258 3 253 1.19e-123 352
MS.gene29490.t1 AT5G56320 67.611 247 76 2 13 259 11 253 6.88e-123 350
MS.gene29490.t1 AT5G56320 67.611 247 76 2 13 259 11 253 6.88e-123 350
MS.gene29490.t1 AT5G05290 73.246 228 58 2 31 257 30 255 7.67e-123 350
MS.gene29490.t1 AT5G56320 67.611 247 76 2 13 259 11 253 6.31e-122 350
MS.gene29490.t1 AT2G40610 66.406 256 75 3 3 257 8 253 7.98e-120 342
MS.gene29490.t1 AT1G26770 70.040 247 71 2 12 258 6 249 3.25e-119 341
MS.gene29490.t1 AT1G26770 70.040 247 71 2 12 258 16 259 3.31e-119 341
MS.gene29490.t1 AT4G01630 62.000 250 91 3 6 252 1 249 6.36e-111 320
MS.gene29490.t1 AT3G29030 67.699 226 64 2 33 258 38 254 5.25e-110 318
MS.gene29490.t1 AT1G69530 62.400 250 62 2 9 258 3 220 2.93e-108 312
MS.gene29490.t1 AT1G69530 63.454 249 57 4 10 258 6 220 2.85e-107 310
MS.gene29490.t1 AT1G20190 63.470 219 77 2 37 253 30 247 7.46e-101 294
MS.gene29490.t1 AT5G39270 59.193 223 87 2 33 255 58 276 1.94e-98 289
MS.gene29490.t1 AT5G39290 58.744 223 88 2 33 255 58 276 6.72e-98 288
MS.gene29490.t1 AT1G62980 55.645 248 103 3 9 255 12 253 3.31e-96 283
MS.gene29490.t1 AT5G39280 56.696 224 93 2 32 255 53 272 4.49e-94 278
MS.gene29490.t1 AT5G39300 56.250 224 94 2 32 255 54 273 2.21e-93 276
MS.gene29490.t1 AT1G12560 53.175 252 114 4 9 258 13 262 3.08e-93 275
MS.gene29490.t1 AT3G03220 54.425 226 102 1 33 258 40 264 2.70e-88 263
MS.gene29490.t1 AT5G39310 54.018 224 98 3 33 255 90 309 2.29e-87 262
MS.gene29490.t1 AT1G20190 59.574 188 73 2 68 253 2 188 8.95e-80 239
MS.gene29490.t1 AT5G39260 50.394 254 111 5 6 255 17 259 4.91e-79 239
MS.gene29490.t1 AT3G15370 48.047 256 120 7 4 255 1 247 1.90e-78 237
MS.gene29490.t1 AT4G38210 46.371 248 131 1 13 258 8 255 9.22e-78 236
MS.gene29490.t1 AT3G15370 43.369 279 117 7 11 255 6 277 4.85e-72 222
MS.gene29490.t1 AT2G20750 29.304 273 159 12 6 257 8 267 6.84e-24 97.4
MS.gene29490.t1 AT4G28250 28.839 267 161 12 6 257 8 260 5.22e-23 95.1
MS.gene29490.t1 AT1G65680 30.638 235 134 11 33 252 47 267 4.06e-22 92.8
MS.gene29490.t1 AT4G28250 27.757 263 158 11 6 255 8 251 5.68e-20 86.7
MS.gene29490.t1 AT2G45110 29.707 239 127 11 35 252 35 253 4.30e-19 84.3
MS.gene29490.t1 AT1G65681 28.767 219 129 9 47 252 12 216 1.76e-18 82.0
MS.gene29490.t1 AT4G17030 26.800 250 160 9 13 252 7 243 2.65e-16 76.6
MS.gene29490.t1 AT3G60570 27.068 266 163 10 10 259 9 259 5.47e-16 75.9
MS.gene29490.t1 AT3G45970 25.352 213 137 9 51 252 14 215 6.09e-15 72.4
MS.gene29490.t1 AT2G20750 27.536 207 123 7 6 196 8 203 3.38e-14 70.1
MS.gene29490.t1 AT4G28250 28.780 205 116 9 6 199 8 193 1.97e-13 67.8
MS.gene29490.t1 AT4G28250 29.814 161 90 6 6 157 8 154 4.02e-13 67.0
MS.gene29490.t1 AT3G45970 24.519 208 135 9 56 252 46 242 2.79e-12 65.5
MS.gene29490.t1 AT3G45960 26.316 209 129 11 56 252 46 241 1.05e-11 63.9

Find 73 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAATATTGTTCCCTCTAAT+TGG 0.218483 5.3:-64632941 MS.gene29490:CDS
TCACTTGGTTGAGCAAAATT+TGG 0.226173 5.3:+64633861 None:intergenic
GTTCCCTCTAATTGGCAATT+TGG 0.258104 5.3:-64632933 MS.gene29490:CDS
TTGGCAATCAAATTCTGTTT+TGG 0.280999 5.3:-64633021 MS.gene29490:CDS
ATAAACGTACTTCAACTTCT+TGG 0.285149 5.3:-64632962 MS.gene29490:CDS
ACCAATATTGAAGGACTTCC+AGG 0.314188 5.3:+64633906 None:intergenic
TTTGCTCAACCAAGTGATAA+TGG 0.320638 5.3:-64633855 MS.gene29490:CDS
CCCTGGAAGTCCTTCAATAT+TGG 0.339675 5.3:-64633907 MS.gene29490:CDS
GGATGAGTATGAGTCGTAAC+TGG 0.344835 5.3:-64633052 MS.gene29490:CDS
TTGACCAAATTGCCAATTAG+AGG 0.346926 5.3:+64632929 None:intergenic
TTAGGTGGAGCATGTGGATA+TGG 0.347554 5.3:-64634053 MS.gene29490:intron
ATAGTGAAGACTAGTGTTAA+AGG 0.372536 5.3:-64633089 MS.gene29490:CDS
AAGGGCTAAGAAGGTGAAAA+TGG 0.373363 5.3:+64634345 None:intergenic
CTAGTACTGACCCATTGTTC+CGG 0.407475 5.3:+64634228 None:intergenic
GGCGTCGCTGCCGCCGTAGA+AGG 0.408061 5.3:+64634252 None:intergenic
GGTGTAGACACCGGGGAGTC+TGG 0.413047 5.3:+64634303 None:intergenic
AGTGAGGAGAAGGGCTAAGA+AGG 0.427838 5.3:+64634336 None:intergenic
CAATTTGGTCAAACTTTCAC+TGG 0.428540 5.3:-64632918 MS.gene29490:CDS
AATTTGTATAGTCAAGGTTA+TGG 0.433320 5.3:-64634029 MS.gene29490:CDS
AGTTACCTGCGGTAAGAAAC+AGG 0.439603 5.3:+64633747 None:intergenic
GTAGCAGTTACCAATATTGA+AGG 0.440044 5.3:+64633897 None:intergenic
CACCAACCTCCATTATCACT+TGG 0.451469 5.3:+64633846 None:intergenic
ACCGCTCACGCCACCTTCTA+CGG 0.458135 5.3:-64634265 MS.gene29490:CDS
ATCACCAACGTTGCAGGTGC+AGG 0.458539 5.3:-64633116 MS.gene29490:CDS
CGGCTTCCGTTACTTCAACT+TGG 0.462141 5.3:-64633144 MS.gene29490:CDS
GACACCGGGGAGTCTGGCTT+CGG 0.467921 5.3:+64634309 None:intergenic
GTGGCACCAACTAGGATCAT+TGG 0.475998 5.3:+64633929 None:intergenic
AGCGGTTTGCCATGCTCCAC+CGG 0.481196 5.3:+64634282 None:intergenic
CTTGGTTGAGCAAAATTTGG+TGG 0.486783 5.3:+64633864 None:intergenic
ATCACCTGCACCTGCAACGT+TGG 0.490975 5.3:+64633112 None:intergenic
AGTGTTCCAATGATCCTAGT+TGG 0.492135 5.3:-64633935 MS.gene29490:CDS
TACGGCGGCAGCGACGCCTC+CGG 0.495150 5.3:-64634247 MS.gene29490:CDS
AGTTGAAGTACGTTTATCAC+TGG 0.500062 5.3:+64632968 None:intergenic
AGCGACGCCTCCGGAACAAT+GGG 0.500990 5.3:-64634238 MS.gene29490:intron
CAGCGACGCCTCCGGAACAA+TGG 0.502237 5.3:-64634239 MS.gene29490:CDS
TTGTTCCTGTTTCTTACCGC+AGG 0.518212 5.3:-64633752 MS.gene29490:intron
CTTCGGTTGAAGTGAGGAGA+AGG 0.527312 5.3:+64634326 None:intergenic
TGCTCCACCGGTGTAGACAC+CGG 0.531092 5.3:+64634294 None:intergenic
AAAATTGCTCAATATCGTGC+TGG 0.536520 5.3:-64633777 MS.gene29490:CDS
GCAAGATCAAAGTGTGGTCG+TGG 0.539841 5.3:+64633816 None:intergenic
TGACCAAATTGCCAATTAGA+GGG 0.540758 5.3:+64632930 None:intergenic
GCTCCACCGGTGTAGACACC+GGG 0.541991 5.3:+64634295 None:intergenic
TATGGAAATTTGTATAGTCA+AGG 0.544667 5.3:-64634035 MS.gene29490:CDS
CTTCCAGGGTGGCACCAACT+AGG 0.546110 5.3:+64633921 None:intergenic
GTGTCTACACCGGTGGAGCA+TGG 0.555010 5.3:-64634291 MS.gene29490:CDS
GTCGCTGCCGCCGTAGAAGG+TGG 0.555378 5.3:+64634255 None:intergenic
GATGAGTATGAGTCGTAACT+GGG 0.556251 5.3:-64633051 MS.gene29490:CDS
GCCGTAGAAGGTGGCGTGAG+CGG 0.563764 5.3:+64634264 None:intergenic
GATCCTAGTTGGTGCCACCC+TGG 0.564618 5.3:-64633924 MS.gene29490:CDS
GGAATAAGATACACAATCAA+CGG 0.582244 5.3:-64633164 MS.gene29490:CDS
AGGTGAAAATGGTGGTGATG+TGG 0.582302 5.3:+64634356 None:intergenic
CTCCCCGGTGTCTACACCGG+TGG 0.584447 5.3:-64634298 MS.gene29490:CDS
TCAACCGAAGCCAGACTCCC+CGG 0.594710 5.3:-64634313 MS.gene29490:CDS
TTAAAACCAAGTTGAAGTAA+CGG 0.599283 5.3:+64633138 None:intergenic
TTCAACAACGGTTTAAGCTG+TGG 0.604039 5.3:-64633975 MS.gene29490:CDS
AGGGTTCCATGCAGAAAACA+AGG 0.610579 5.3:-64633188 MS.gene29490:CDS
AACCAAGTGATAATGGAGGT+TGG 0.617066 5.3:-64633848 MS.gene29490:CDS
GTCTGGCTTCGGTTGAAGTG+AGG 0.619944 5.3:+64634320 None:intergenic
AGACTCCCCGGTGTCTACAC+CGG 0.622174 5.3:-64634301 MS.gene29490:CDS
CCAATATTGAAGGACTTCCA+GGG 0.631547 5.3:+64633907 None:intergenic
GCTCACGCCACCTTCTACGG+CGG 0.631792 5.3:-64634262 MS.gene29490:CDS
TTCGGTTGAAGTGAGGAGAA+GGG 0.636772 5.3:+64634327 None:intergenic
AGCACAGCACTATTCAACAA+CGG 0.641968 5.3:-64633987 MS.gene29490:CDS
GCTCAACCAAGTGATAATGG+AGG 0.648199 5.3:-64633852 MS.gene29490:CDS
GGCTAAGAAGGTGAAAATGG+TGG 0.650038 5.3:+64634348 None:intergenic
AGATCAAAGTGTGGTCGTGG+AGG 0.655541 5.3:+64633819 None:intergenic
GTACTGACCCATTGTTCCGG+AGG 0.658317 5.3:+64634231 None:intergenic
TGAGTATGAGTCGTAACTGG+GGG 0.680229 5.3:-64633049 MS.gene29490:CDS
ATGAGTATGAGTCGTAACTG+GGG 0.683363 5.3:-64633050 MS.gene29490:CDS
GGCATAGCAAGATCAAAGTG+TGG 0.696702 5.3:+64633810 None:intergenic
CTCCACCGGTGTAGACACCG+GGG 0.757850 5.3:+64634296 None:intergenic
GTTCCATGCAGAAAACAAGG+AGG 0.759316 5.3:-64633185 MS.gene29490:CDS
ATATTGAAGGACTTCCAGGG+TGG 0.792160 5.3:+64633910 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATTTTTATTTATTTTTT+TGG - chr5.3:64633663-64633682 MS.gene29490:intron 0.0%
!! ACAAAAAATAAATAAAATTG+TGG + chr5.3:64633087-64633106 None:intergenic 10.0%
!! ATAACTAAATAAAATAAAAG+AGG + chr5.3:64633789-64633808 None:intergenic 10.0%
!!! AAGAAATATTAATGAAATTT+TGG + chr5.3:64633564-64633583 None:intergenic 10.0%
!!! TTAAATTTTGGTTACATTTT+AGG - chr5.3:64633195-64633214 MS.gene29490:intron 15.0%
!! AAATAAATAAAATTGTGGCA+TGG + chr5.3:64633082-64633101 None:intergenic 20.0%
!! GAGATGTATAAAATAAACTT+AGG + chr5.3:64633602-64633621 None:intergenic 20.0%
!! TAAATAAAATAAAAGAGGCA+TGG + chr5.3:64633784-64633803 None:intergenic 20.0%
!! TCGATATTGTTTGATTAAAA+TGG - chr5.3:64633834-64633853 MS.gene29490:CDS 20.0%
!! TTATACATCTCTCAAAAAAT+TGG - chr5.3:64633611-64633630 MS.gene29490:intron 20.0%
!!! AAAAAGAGAATATTTTTGCA+AGG + chr5.3:64633741-64633760 None:intergenic 20.0%
!!! TTTGTGCTAATGTTAAATTT+TGG - chr5.3:64633183-64633202 MS.gene29490:CDS 20.0%
! AATTTGTATAGTCAAGGTTA+TGG - chr5.3:64633237-64633256 MS.gene29490:intron 25.0%
! GTAATAAAACACAACATCAT+GGG + chr5.3:64634011-64634030 None:intergenic 25.0%
! TATGGAAATTTGTATAGTCA+AGG - chr5.3:64633231-64633250 MS.gene29490:intron 25.0%
! TGTAATAAAACACAACATCA+TGG + chr5.3:64634012-64634031 None:intergenic 25.0%
!! TTAAAACCAAGTTGAAGTAA+CGG + chr5.3:64634131-64634150 None:intergenic 25.0%
!!! AATTTTGGTTACATTTTAGG+TGG - chr5.3:64633198-64633217 MS.gene29490:intron 25.0%
AAATAAAATTGTGGCATGGT+TGG + chr5.3:64633078-64633097 None:intergenic 30.0%
AGTGTTAAAGGTTCAAAAAC+TGG - chr5.3:64634189-64634208 MS.gene29490:intron 30.0%
ATAAACGTACTTCAACTTCT+TGG - chr5.3:64634304-64634323 MS.gene29490:CDS 30.0%
ATAGTGAAGACTAGTGTTAA+AGG - chr5.3:64634177-64634196 MS.gene29490:intron 30.0%
GGAATAAGATACACAATCAA+CGG - chr5.3:64634102-64634121 MS.gene29490:intron 30.0%
TAAACAAGAATAGTTACCTG+CGG + chr5.3:64633533-64633552 None:intergenic 30.0%
TACATCTCTCAAAAAATTGG+TGG - chr5.3:64633614-64633633 MS.gene29490:intron 30.0%
TTAAAGGTTCAAAAACTGGT+TGG - chr5.3:64634193-64634212 MS.gene29490:intron 30.0%
! TGGTCAATCACTTTCTTTTA+GGG - chr5.3:64634269-64634288 MS.gene29490:CDS 30.0%
! TTGGCAATCAAATTCTGTTT+TGG - chr5.3:64634245-64634264 MS.gene29490:CDS 30.0%
! TTGGTCAATCACTTTCTTTT+AGG - chr5.3:64634268-64634287 MS.gene29490:CDS 30.0%
!!! CAATCAAATTCTGTTTTGGT+TGG - chr5.3:64634249-64634268 MS.gene29490:CDS 30.0%
AAAATTGCTCAATATCGTGC+TGG - chr5.3:64633489-64633508 MS.gene29490:intron 35.0%
AGTTGAAGTACGTTTATCAC+TGG + chr5.3:64634301-64634320 None:intergenic 35.0%
CAATTTGGTCAAACTTTCAC+TGG - chr5.3:64634348-64634367 MS.gene29490:CDS 35.0%
GGAATATTGTTCCCTCTAAT+TGG - chr5.3:64634325-64634344 MS.gene29490:CDS 35.0%
GTAGCAGTTACCAATATTGA+AGG + chr5.3:64633372-64633391 None:intergenic 35.0%
GTTTGTCACAAAACTTGAAC+CGG + chr5.3:64633881-64633900 None:intergenic 35.0%
TCACTTGGTTGAGCAAAATT+TGG + chr5.3:64633408-64633427 None:intergenic 35.0%
TGACCAAATTGCCAATTAGA+GGG + chr5.3:64634339-64634358 None:intergenic 35.0%
TTATTATGCGGCAAAATGAG+CGG + chr5.3:64633903-64633922 None:intergenic 35.0%
TTGACCAAATTGCCAATTAG+AGG + chr5.3:64634340-64634359 None:intergenic 35.0%
TTTGCTCAACCAAGTGATAA+TGG - chr5.3:64633411-64633430 MS.gene29490:intron 35.0%
TTTGTCACAAAACTTGAACC+GGG + chr5.3:64633880-64633899 None:intergenic 35.0%
! CACGATATTGAGCAATTTTG+AGG + chr5.3:64633488-64633507 None:intergenic 35.0%
!! TCGTGAGTCTTGTTATTATG+CGG + chr5.3:64633915-64633934 None:intergenic 35.0%
!! TGAGCAATTTTGAGGAACAT+AGG + chr5.3:64633480-64633499 None:intergenic 35.0%
AAGGGCTAAGAAGGTGAAAA+TGG + chr5.3:64632924-64632943 None:intergenic 40.0%
ACCAATATTGAAGGACTTCC+AGG + chr5.3:64633363-64633382 None:intergenic 40.0%
AGCACAGCACTATTCAACAA+CGG - chr5.3:64633279-64633298 MS.gene29490:intron 40.0%
AGTGTTCCAATGATCCTAGT+TGG - chr5.3:64633331-64633350 MS.gene29490:intron 40.0%
ATGAGTATGAGTCGTAACTG+GGG - chr5.3:64634216-64634235 MS.gene29490:intron 40.0%
CCAATATTGAAGGACTTCCA+GGG + chr5.3:64633362-64633381 None:intergenic 40.0%
CTTGGTTGAGCAAAATTTGG+TGG + chr5.3:64633405-64633424 None:intergenic 40.0%
GATGAGTATGAGTCGTAACT+GGG - chr5.3:64634215-64634234 MS.gene29490:intron 40.0%
GTTCCCTCTAATTGGCAATT+TGG - chr5.3:64634333-64634352 MS.gene29490:CDS 40.0%
TGTCCTTTGATGTGCATGTA+GGG - chr5.3:64634059-64634078 MS.gene29490:intron 40.0%
TTCAACAACGGTTTAAGCTG+TGG - chr5.3:64633291-64633310 MS.gene29490:intron 40.0%
TTGAACCGGGTTTAGATGAA+CGG + chr5.3:64633867-64633886 None:intergenic 40.0%
! GACACGAGTATTTTCAGCAA+GGG + chr5.3:64634043-64634062 None:intergenic 40.0%
!! AACCAAGTGATAATGGAGGT+TGG - chr5.3:64633418-64633437 MS.gene29490:intron 40.0%
!! GTTTTAATCACCAACGTTGC+AGG - chr5.3:64634144-64634163 MS.gene29490:intron 40.0%
!! TACATTTTAGGTGGAGCATG+TGG - chr5.3:64633207-64633226 MS.gene29490:intron 40.0%
!!! ATTCCTCCTTGTTTTCTGCA+TGG + chr5.3:64634087-64634106 None:intergenic 40.0%
AGGGTTCCATGCAGAAAACA+AGG - chr5.3:64634078-64634097 MS.gene29490:intron 45.0%
AGTTACCTGCGGTAAGAAAC+AGG + chr5.3:64633522-64633541 None:intergenic 45.0%
ATATTGAAGGACTTCCAGGG+TGG + chr5.3:64633359-64633378 None:intergenic 45.0%
CACCAACCTCCATTATCACT+TGG + chr5.3:64633423-64633442 None:intergenic 45.0%
CCCTGGAAGTCCTTCAATAT+TGG - chr5.3:64633359-64633378 MS.gene29490:intron 45.0%
CTGAACCGTTCATCTAAACC+CGG - chr5.3:64633859-64633878 MS.gene29490:CDS 45.0%
GAACCCTACATGCACATCAA+AGG + chr5.3:64634065-64634084 None:intergenic 45.0%
GACGCAAGTTTGAATCTGCT+AGG + chr5.3:64633119-64633138 None:intergenic 45.0%
GGATGAGTATGAGTCGTAAC+TGG - chr5.3:64634214-64634233 MS.gene29490:intron 45.0%
GGCATAGCAAGATCAAAGTG+TGG + chr5.3:64633459-64633478 None:intergenic 45.0%
GGCTAAGAAGGTGAAAATGG+TGG + chr5.3:64632921-64632940 None:intergenic 45.0%
GTGTCCTTTGATGTGCATGT+AGG - chr5.3:64634058-64634077 MS.gene29490:intron 45.0%
GTTCCATGCAGAAAACAAGG+AGG - chr5.3:64634081-64634100 MS.gene29490:intron 45.0%
TGAGTATGAGTCGTAACTGG+GGG - chr5.3:64634217-64634236 MS.gene29490:intron 45.0%
TTAGGTGGAGCATGTGGATA+TGG - chr5.3:64633213-64633232 MS.gene29490:intron 45.0%
TTCGGTTGAAGTGAGGAGAA+GGG + chr5.3:64632942-64632961 None:intergenic 45.0%
! AGGTGAAAATGGTGGTGATG+TGG + chr5.3:64632913-64632932 None:intergenic 45.0%
! GGACACGAGTATTTTCAGCA+AGG + chr5.3:64634044-64634063 None:intergenic 45.0%
! TTGTTCCTGTTTCTTACCGC+AGG - chr5.3:64633514-64633533 MS.gene29490:intron 45.0%
!! CTAGTACTGACCCATTGTTC+CGG + chr5.3:64633041-64633060 None:intergenic 45.0%
!! GCTCAACCAAGTGATAATGG+AGG - chr5.3:64633414-64633433 MS.gene29490:intron 45.0%
AGATCAAAGTGTGGTCGTGG+AGG + chr5.3:64633450-64633469 None:intergenic 50.0%
AGTGAGGAGAAGGGCTAAGA+AGG + chr5.3:64632933-64632952 None:intergenic 50.0%
CGGCTTCCGTTACTTCAACT+TGG - chr5.3:64634122-64634141 MS.gene29490:intron 50.0%
CTTCGGTTGAAGTGAGGAGA+AGG + chr5.3:64632943-64632962 None:intergenic 50.0%
GCAAGATCAAAGTGTGGTCG+TGG + chr5.3:64633453-64633472 None:intergenic 50.0%
GTCGTAACTGGGGGCAAAAT+TGG - chr5.3:64634226-64634245 MS.gene29490:intron 50.0%
GTGGCACCAACTAGGATCAT+TGG + chr5.3:64633340-64633359 None:intergenic 50.0%
ATCACCAACGTTGCAGGTGC+AGG - chr5.3:64634150-64634169 MS.gene29490:intron 55.0%
ATCACCTGCACCTGCAACGT+TGG + chr5.3:64634157-64634176 None:intergenic 55.0%
! GTACTGACCCATTGTTCCGG+AGG + chr5.3:64633038-64633057 None:intergenic 55.0%
! GTCTGGCTTCGGTTGAAGTG+AGG + chr5.3:64632949-64632968 None:intergenic 55.0%
ACCGCTCACGCCACCTTCTA+CGG - chr5.3:64633001-64633020 MS.gene29490:CDS 60.0%
AGACTCCCCGGTGTCTACAC+CGG - chr5.3:64632965-64632984 MS.gene29490:CDS 60.0%
AGCGACGCCTCCGGAACAAT+GGG - chr5.3:64633028-64633047 MS.gene29490:CDS 60.0%
AGCGGTTTGCCATGCTCCAC+CGG + chr5.3:64632987-64633006 None:intergenic 60.0%
CTTCCAGGGTGGCACCAACT+AGG + chr5.3:64633348-64633367 None:intergenic 60.0%
GTGTCTACACCGGTGGAGCA+TGG - chr5.3:64632975-64632994 MS.gene29490:CDS 60.0%
TCAACCGAAGCCAGACTCCC+CGG - chr5.3:64632953-64632972 MS.gene29490:CDS 60.0%
TGCTCCACCGGTGTAGACAC+CGG + chr5.3:64632975-64632994 None:intergenic 60.0%
!! GATCCTAGTTGGTGCCACCC+TGG - chr5.3:64633342-64633361 MS.gene29490:intron 60.0%
CAGCGACGCCTCCGGAACAA+TGG - chr5.3:64633027-64633046 MS.gene29490:CDS 65.0%
CTCCACCGGTGTAGACACCG+GGG + chr5.3:64632973-64632992 None:intergenic 65.0%
GCCGTAGAAGGTGGCGTGAG+CGG + chr5.3:64633005-64633024 None:intergenic 65.0%
GCTCACGCCACCTTCTACGG+CGG - chr5.3:64633004-64633023 MS.gene29490:CDS 65.0%
GCTCCACCGGTGTAGACACC+GGG + chr5.3:64632974-64632993 None:intergenic 65.0%
GGTGTAGACACCGGGGAGTC+TGG + chr5.3:64632966-64632985 None:intergenic 65.0%
! GACACCGGGGAGTCTGGCTT+CGG + chr5.3:64632960-64632979 None:intergenic 65.0%
CTCCCCGGTGTCTACACCGG+TGG - chr5.3:64632968-64632987 MS.gene29490:CDS 70.0%
GTCGCTGCCGCCGTAGAAGG+TGG + chr5.3:64633014-64633033 None:intergenic 70.0%
GGCGTCGCTGCCGCCGTAGA+AGG + chr5.3:64633017-64633036 None:intergenic 75.0%
TACGGCGGCAGCGACGCCTC+CGG - chr5.3:64633019-64633038 MS.gene29490:CDS 75.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 64632899 64634389 64632899 ID=MS.gene29490
chr5.3 mRNA 64632899 64634389 64632899 ID=MS.gene29490.t1;Parent=MS.gene29490
chr5.3 exon 64634239 64634389 64634239 ID=MS.gene29490.t1.exon1;Parent=MS.gene29490.t1
chr5.3 CDS 64634239 64634389 64634239 ID=cds.MS.gene29490.t1;Parent=MS.gene29490.t1
chr5.3 exon 64633753 64634071 64633753 ID=MS.gene29490.t1.exon2;Parent=MS.gene29490.t1
chr5.3 CDS 64633753 64634071 64633753 ID=cds.MS.gene29490.t1;Parent=MS.gene29490.t1
chr5.3 exon 64632899 64633208 64632899 ID=MS.gene29490.t1.exon3;Parent=MS.gene29490.t1
chr5.3 CDS 64632899 64633208 64632899 ID=cds.MS.gene29490.t1;Parent=MS.gene29490.t1
Gene Sequence

>MS.gene29490

ATGACTAAGCTCCACATCACCACCATTTTCACCTTCTTAGCCCTTCTCCTCACTTCAACCGAAGCCAGACTCCCCGGTGTCTACACCGGTGGAGCATGGCAAACCGCTCACGCCACCTTCTACGGCGGCAGCGACGCCTCCGGAACAATGGGTCAGTACTAGAAAAAAAAATATTACCAACCATGCCACAATTTTATTTATTTTTTGTTCTAAAATACCTAGCAGATTCAAACTTGCGTCGCAACATATCAAATGTCTCTGATATCTTCGTTTAATAATGTTCTTTTGTGCTAATGTTAAATTTTGGTTACATTTTAGGTGGAGCATGTGGATATGGAAATTTGTATAGTCAAGGTTATGGTGTGAACACAGCAGCATTAAGCACAGCACTATTCAACAACGGTTTAAGCTGTGGCTCGTGTTTTGAGTTAAAGTGTTCCAATGATCCTAGTTGGTGCCACCCTGGAAGTCCTTCAATATTGGTAACTGCTACAAATTTTTGTCCACCAAATTTTGCTCAACCAAGTGATAATGGAGGTTGGTGTAATCCTCCACGACCACACTTTGATCTTGCTATGCCTATGTTCCTCAAAATTGCTCAATATCGTGCTGGTATTGTTCCTGTTTCTTACCGCAGGTAACTATTCTTGTTTACTTTCACTCCAAAATTTCATTAATATTTCTTTGTTGATAAAATAAGCCTAAGTTTATTTTATACATCTCTCAAAAAATTGGTGGAAATAGTAGTTGATGATGATTGTTCTATTATTTTTATTTATTTTTTTGGTCAAGTGTTCTATTAATTTATTTTATTTCATATTTTTTAATCTTGTAGCATTCCTTGCAAAAATATTCTCTTTTTCTTTTTTACATTTTTCTTTACCATGCCTCTTTTATTTTATTTAGTTATCTTAAGTCTATCTCAATTGATAATATCGATATTGTTTGATTAAAATGGATCTGAACCGTTCATCTAAACCCGGTTCAAGTTTTGTGACAAACCGCTCATTTTGCCGCATAATAACAAGACTCACGAAACACAACAAAATTTAGAATCTACTTTTCTGTCACGCTTGTACTATACTAGTACAAGTTAACATATTTTATTGCCCATGATGTTGTGTTTTATTACAACTTTATACCCTTGCTGAAAATACTCGTGTCCTTTGATGTGCATGTAGGGTTCCATGCAGAAAACAAGGAGGAATAAGATACACAATCAACGGCTTCCGTTACTTCAACTTGGTTTTAATCACCAACGTTGCAGGTGCAGGTGATATAGTGAAGACTAGTGTTAAAGGTTCAAAAACTGGTTGGATGAGTATGAGTCGTAACTGGGGGCAAAATTGGCAATCAAATTCTGTTTTGGTTGGTCAATCACTTTCTTTTAGGGTTACATCCAGTGATAAACGTACTTCAACTTCTTGGAATATTGTTCCCTCTAATTGGCAATTTGGTCAAACTTTCACTGGCAAGAATTTTCGAGTCTAA
Protein sequence

>MS.gene29490.t1

MTKLHITTIFTFLALLLTSTEARLPGVYTGGAWQTAHATFYGGSDASGTMGGACGYGNLYSQGYGVNTAALSTALFNNGLSCGSCFELKCSNDPSWCHPGSPSILVTATNFCPPNFAQPSDNGGWCNPPRPHFDLAMPMFLKIAQYRAGIVPVSYRRVPCRKQGGIRYTINGFRYFNLVLITNVAGAGDIVKTSVKGSKTGWMSMSRNWGQNWQSNSVLVGQSLSFRVTSSDKRTSTSWNIVPSNWQFGQTFTGKNFRV