Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069093.t1 | XP_013453681.1 | 97.5 | 120 | 3 | 0 | 2 | 121 | 1 | 120 | 1.10E-47 | 199.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069093.t1 | A0A072UDK7 | 97.5 | 120 | 3 | 0 | 2 | 121 | 1 | 120 | 8.3e-48 | 199.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052211 | MS.gene069093 | 0.801918 | 7.18E-49 | -1.69E-46 |
| MS.gene052606 | MS.gene069093 | 0.825595 | 4.30E-54 | -1.69E-46 |
| MS.gene053421 | MS.gene069093 | 0.810559 | 1.09E-50 | -1.69E-46 |
| MS.gene055158 | MS.gene069093 | 0.844791 | 6.07E-59 | -1.69E-46 |
| MS.gene056748 | MS.gene069093 | 0.820122 | 8.10E-53 | -1.69E-46 |
| MS.gene056988 | MS.gene069093 | 0.806017 | 1.01E-49 | -1.69E-46 |
| MS.gene057768 | MS.gene069093 | 0.850721 | 1.41E-60 | -1.69E-46 |
| MS.gene058111 | MS.gene069093 | 0.831811 | 1.35E-55 | -1.69E-46 |
| MS.gene06077 | MS.gene069093 | -0.807883 | 4.07E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069093.t1 | MTR_5g030310 | 99.167 | 120 | 1 | 0 | 2 | 121 | 1 | 120 | 8.85e-83 | 238 |
| MS.gene069093.t1 | MTR_5g430440 | 98.333 | 120 | 2 | 0 | 2 | 121 | 1 | 120 | 8.58e-82 | 235 |
| MS.gene069093.t1 | MTR_5g430430 | 83.333 | 120 | 20 | 0 | 2 | 121 | 1 | 120 | 8.71e-69 | 202 |
| MS.gene069093.t1 | MTR_5g030290 | 83.333 | 120 | 20 | 0 | 2 | 121 | 1 | 120 | 8.71e-69 | 202 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGGCCTTCAGTCTTGAAAA+TGG | 0.356723 | 5.3:+19136647 | None:intergenic |
| TTATGCAGTGATGGTGGAGC+TGG | 0.397441 | 5.3:-19136744 | MS.gene069093:intron |
| CTTGCTCTTGAGTGAAGCTC+TGG | 0.411535 | 5.3:+19137613 | None:intergenic |
| TCACTGCTACTCTCAATAAC+TGG | 0.438274 | 5.3:+19136717 | None:intergenic |
| CCCTTGCCAGAGTTTACACC+AGG | 0.459349 | 5.3:+19136627 | None:intergenic |
| GGCAATGTGTTGTGCAAGAC+TGG | 0.486701 | 5.3:-19137379 | MS.gene069093:CDS |
| AGGACTGAAGGGCCTGATGC+TGG | 0.490624 | 5.3:-19137331 | MS.gene069093:CDS |
| ACAACACATTGCCCAAGCCC+TGG | 0.525921 | 5.3:+19137389 | None:intergenic |
| CCCTGGTGTAAACTCTGGCA+AGG | 0.541663 | 5.3:-19136628 | MS.gene069093:CDS |
| TATGCAGTGATGGTGGAGCT+GGG | 0.557903 | 5.3:-19136743 | MS.gene069093:intron |
| ACATTCTGCAAGTGTGGTGA+AGG | 0.561546 | 5.3:-19136681 | MS.gene069093:CDS |
| ATTGAGAGTAGCAGTGACAA+TGG | 0.563224 | 5.3:-19136711 | MS.gene069093:CDS |
| CAGAGCTTCACTCAAGAGCA+AGG | 0.567749 | 5.3:-19137612 | MS.gene069093:CDS |
| TTGCTCTTGAGTGAAGCTCT+GGG | 0.573531 | 5.3:+19137614 | None:intergenic |
| GTTGGACATGTGTTATAACT+AGG | 0.581816 | 5.3:-19137351 | MS.gene069093:CDS |
| GTGTTATAACTAGGACTGAA+GGG | 0.595455 | 5.3:-19137342 | MS.gene069093:CDS |
| AAACTCTTATGCAGTGATGG+TGG | 0.602169 | 5.3:-19136750 | MS.gene069093:intron |
| GAAGGCCCTGGTGTAAACTC+TGG | 0.603912 | 5.3:-19136633 | MS.gene069093:CDS |
| TGTGTTATAACTAGGACTGA+AGG | 0.610701 | 5.3:-19137343 | MS.gene069093:CDS |
| CCTGGTGTAAACTCTGGCAA+GGG | 0.620143 | 5.3:-19136627 | MS.gene069093:CDS |
| GTGTTGTGCAAGACTGGTAA+AGG | 0.626364 | 5.3:-19137373 | MS.gene069093:CDS |
| AGAGCTTCACTCAAGAGCAA+GGG | 0.651871 | 5.3:-19137611 | MS.gene069093:intron |
| TCTGCAAGTGTGGTGAAGGG+TGG | 0.652623 | 5.3:-19136677 | MS.gene069093:CDS |
| TGTGCAAGACTGGTAAAGGT+TGG | 0.662647 | 5.3:-19137369 | MS.gene069093:CDS |
| AGTGAAACATTCTGCAAGTG+TGG | 0.671634 | 5.3:-19136687 | MS.gene069093:CDS |
| CATTCTGCAAGTGTGGTGAA+GGG | 0.681189 | 5.3:-19136680 | MS.gene069093:CDS |
| CCTTGCCAGAGTTTACACCA+GGG | 0.773669 | 5.3:+19136628 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAATTATTTGTTTGTAATA+TGG | - | chr5.3:19137154-19137173 | MS.gene069093:intron | 10.0% |
| !! | TAATATGAAATTGAGATTTA+AGG | + | chr5.3:19137279-19137298 | None:intergenic | 15.0% |
| !! | AAACTGTAAAGATGAAATAA+AGG | + | chr5.3:19136651-19136670 | None:intergenic | 20.0% |
| !! | AATTTATCGTTCAACTTATT+AGG | + | chr5.3:19137239-19137258 | None:intergenic | 20.0% |
| !! | GCAGTTAAAATAATAATAAG+AGG | + | chr5.3:19136959-19136978 | None:intergenic | 20.0% |
| !! | TAAAATAATAATAAGAGGAG+TGG | + | chr5.3:19136954-19136973 | None:intergenic | 20.0% |
| !! | TTATTAGGTAAAAGTTCAAT+AGG | + | chr5.3:19137224-19137243 | None:intergenic | 20.0% |
| ! | AAAGTGTGAAAGACTATATT+AGG | - | chr5.3:19137085-19137104 | MS.gene069093:intron | 25.0% |
| ! | ATTATGACAGGTATGAAAAA+TGG | + | chr5.3:19136745-19136764 | None:intergenic | 25.0% |
| ! | TGTAAAGATGAAATAAAGGA+TGG | + | chr5.3:19136647-19136666 | None:intergenic | 25.0% |
| CTAAAATGTCAAATACTGCT+TGG | + | chr5.3:19137030-19137049 | None:intergenic | 30.0% | |
| ! | AAAAATGGAGCGCATTATTT+TGG | + | chr5.3:19136730-19136749 | None:intergenic | 30.0% |
| ! | GCTTATTGGTTAATTATGAC+AGG | + | chr5.3:19136757-19136776 | None:intergenic | 30.0% |
| ! | TTAACCAATAAGCTTTGTGT+AGG | - | chr5.3:19136764-19136783 | MS.gene069093:intron | 30.0% |
| !! | GCAATCATTTTAACAATGGT+TGG | + | chr5.3:19137181-19137200 | None:intergenic | 30.0% |
| !! | TGGTGCAATCATTTTAACAA+TGG | + | chr5.3:19137185-19137204 | None:intergenic | 30.0% |
| ATCAAACTCTTATGCAGTGA+TGG | - | chr5.3:19137452-19137471 | MS.gene069093:intron | 35.0% | |
| CTTTCCTACACAAAGCTTAT+TGG | + | chr5.3:19136771-19136790 | None:intergenic | 35.0% | |
| GTGTTATAACTAGGACTGAA+GGG | - | chr5.3:19136863-19136882 | MS.gene069093:intron | 35.0% | |
| GTTGGACATGTGTTATAACT+AGG | - | chr5.3:19136854-19136873 | MS.gene069093:intron | 35.0% | |
| TAGGTAAGATCTTCATGTCA+TGG | + | chr5.3:19137205-19137224 | None:intergenic | 35.0% | |
| TGTGTTATAACTAGGACTGA+AGG | - | chr5.3:19136862-19136881 | MS.gene069093:intron | 35.0% | |
| ! | CTGAGTTTTTATCTTGAGCT+GGG | + | chr5.3:19136795-19136814 | None:intergenic | 35.0% |
| ! | GGCAAAGTTTTTGCTAAATG+TGG | - | chr5.3:19136895-19136914 | MS.gene069093:intron | 35.0% |
| !! | TGTTCCATTTTCAAGACTGA+AGG | - | chr5.3:19137554-19137573 | MS.gene069093:intron | 35.0% |
| AAACTCTTATGCAGTGATGG+TGG | - | chr5.3:19137455-19137474 | MS.gene069093:intron | 40.0% | |
| AGTGAAACATTCTGCAAGTG+TGG | - | chr5.3:19137518-19137537 | MS.gene069093:intron | 40.0% | |
| ATTGAGAGTAGCAGTGACAA+TGG | - | chr5.3:19137494-19137513 | MS.gene069093:intron | 40.0% | |
| CAAGATAAAAACTCAGGCCA+GGG | - | chr5.3:19136799-19136818 | MS.gene069093:intron | 40.0% | |
| CCAGCTCAAGATAAAAACTC+AGG | - | chr5.3:19136793-19136812 | MS.gene069093:intron | 40.0% | |
| TCAAGATAAAAACTCAGGCC+AGG | - | chr5.3:19136798-19136817 | MS.gene069093:intron | 40.0% | |
| TCACTGCTACTCTCAATAAC+TGG | + | chr5.3:19137491-19137510 | None:intergenic | 40.0% | |
| ! | CCTGAGTTTTTATCTTGAGC+TGG | + | chr5.3:19136796-19136815 | None:intergenic | 40.0% |
| ACATTCTGCAAGTGTGGTGA+AGG | - | chr5.3:19137524-19137543 | MS.gene069093:intron | 45.0% | |
| AGAGCTTCACTCAAGAGCAA+GGG | - | chr5.3:19136594-19136613 | MS.gene069093:CDS | 45.0% | |
| AGGGCCTTCAGTCTTGAAAA+TGG | + | chr5.3:19137561-19137580 | None:intergenic | 45.0% | |
| CATTCTGCAAGTGTGGTGAA+GGG | - | chr5.3:19137525-19137544 | MS.gene069093:intron | 45.0% | |
| GCAAAAACTTTGCCAGCATC+AGG | + | chr5.3:19136889-19136908 | None:intergenic | 45.0% | |
| GTGTTGTGCAAGACTGGTAA+AGG | - | chr5.3:19136832-19136851 | MS.gene069093:intron | 45.0% | |
| TGTGCAAGACTGGTAAAGGT+TGG | - | chr5.3:19136836-19136855 | MS.gene069093:intron | 45.0% | |
| TTGCTCTTGAGTGAAGCTCT+GGG | + | chr5.3:19136594-19136613 | None:intergenic | 45.0% | |
| !! | ATTTTCAAGACTGAAGGCCC+TGG | - | chr5.3:19137560-19137579 | MS.gene069093:intron | 45.0% |
| AAAAACTCAGGCCAGGGCTT+GGG | - | chr5.3:19136805-19136824 | MS.gene069093:intron | 50.0% | |
| CAGAGCTTCACTCAAGAGCA+AGG | - | chr5.3:19136593-19136612 | MS.gene069093:CDS | 50.0% | |
| CCTGGTGTAAACTCTGGCAA+GGG | - | chr5.3:19137578-19137597 | MS.gene069093:intron | 50.0% | |
| CCTTGCCAGAGTTTACACCA+GGG | + | chr5.3:19137580-19137599 | None:intergenic | 50.0% | |
| CTTGCTCTTGAGTGAAGCTC+TGG | + | chr5.3:19136595-19136614 | None:intergenic | 50.0% | |
| GGCAATGTGTTGTGCAAGAC+TGG | - | chr5.3:19136826-19136845 | MS.gene069093:intron | 50.0% | |
| TAAAAACTCAGGCCAGGGCT+TGG | - | chr5.3:19136804-19136823 | MS.gene069093:intron | 50.0% | |
| !! | TATGCAGTGATGGTGGAGCT+GGG | - | chr5.3:19137462-19137481 | MS.gene069093:intron | 50.0% |
| !! | TTATGCAGTGATGGTGGAGC+TGG | - | chr5.3:19137461-19137480 | MS.gene069093:intron | 50.0% |
| ACAACACATTGCCCAAGCCC+TGG | + | chr5.3:19136819-19136838 | None:intergenic | 55.0% | |
| CCCTGGTGTAAACTCTGGCA+AGG | - | chr5.3:19137577-19137596 | MS.gene069093:intron | 55.0% | |
| CCCTTGCCAGAGTTTACACC+AGG | + | chr5.3:19137581-19137600 | None:intergenic | 55.0% | |
| GAAGGCCCTGGTGTAAACTC+TGG | - | chr5.3:19137572-19137591 | MS.gene069093:intron | 55.0% | |
| TCTGCAAGTGTGGTGAAGGG+TGG | - | chr5.3:19137528-19137547 | MS.gene069093:intron | 55.0% | |
| AGGACTGAAGGGCCTGATGC+TGG | - | chr5.3:19136874-19136893 | MS.gene069093:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 19136581 | 19137646 | 19136581 | ID=MS.gene069093 |
| chr5.3 | mRNA | 19136581 | 19137646 | 19136581 | ID=MS.gene069093.t1;Parent=MS.gene069093 |
| chr5.3 | exon | 19137612 | 19137646 | 19137612 | ID=MS.gene069093.t1.exon1;Parent=MS.gene069093.t1 |
| chr5.3 | CDS | 19137612 | 19137646 | 19137612 | ID=cds.MS.gene069093.t1;Parent=MS.gene069093.t1 |
| chr5.3 | exon | 19137289 | 19137441 | 19137289 | ID=MS.gene069093.t1.exon2;Parent=MS.gene069093.t1 |
| chr5.3 | CDS | 19137289 | 19137441 | 19137289 | ID=cds.MS.gene069093.t1;Parent=MS.gene069093.t1 |
| chr5.3 | exon | 19136581 | 19136758 | 19136581 | ID=MS.gene069093.t1.exon3;Parent=MS.gene069093.t1 |
| chr5.3 | CDS | 19136581 | 19136758 | 19136581 | ID=cds.MS.gene069093.t1;Parent=MS.gene069093.t1 |
| Gene Sequence |
| Protein sequence |
