Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069393.t1 | XP_013467605.1 | 89.3 | 131 | 12 | 1 | 5 | 135 | 1010 | 1138 | 1.20E-50 | 209.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069393.t1 | A0A072VIJ0 | 89.3 | 131 | 12 | 1 | 5 | 135 | 1010 | 1138 | 8.9e-51 | 209.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049825 | MS.gene069393 | 0.829059 | 6.36E-55 | -1.69E-46 |
| MS.gene049873 | MS.gene069393 | 0.864276 | 1.37E-64 | -1.69E-46 |
| MS.gene050355 | MS.gene069393 | 0.806765 | 7.02E-50 | -1.69E-46 |
| MS.gene052519 | MS.gene069393 | 0.800751 | 1.24E-48 | -1.69E-46 |
| MS.gene053226 | MS.gene069393 | 0.802411 | 5.68E-49 | -1.69E-46 |
| MS.gene055808 | MS.gene069393 | 0.826503 | 2.62E-54 | -1.69E-46 |
| MS.gene056091 | MS.gene069393 | 0.8055 | 1.30E-49 | -1.69E-46 |
| MS.gene05954 | MS.gene069393 | 0.817296 | 3.55E-52 | -1.69E-46 |
| MS.gene059935 | MS.gene069393 | 0.855639 | 5.51E-62 | -1.69E-46 |
| MS.gene060429 | MS.gene069393 | 0.81241 | 4.30E-51 | -1.69E-46 |
| MS.gene060830 | MS.gene069393 | 0.833785 | 4.37E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069393.t1 | MTR_1g053255 | 89.313 | 131 | 12 | 1 | 5 | 135 | 1010 | 1138 | 8.24e-73 | 235 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGATACAAATTCAAATTTA+AGG | 0.162196 | 1.3:-34399001 | MS.gene069393:CDS |
| TCTTGGGTGTTCGAAATATC+AGG | 0.330937 | 1.3:+34399149 | None:intergenic |
| CTGAAACAAGTCTTTCTCTT+GGG | 0.351101 | 1.3:+34399133 | None:intergenic |
| GCTGAAACAAGTCTTTCTCT+TGG | 0.374263 | 1.3:+34399132 | None:intergenic |
| AAACTGAACCAACTAAGTTT+AGG | 0.377714 | 1.3:-34399067 | MS.gene069393:CDS |
| TCATTGCTCCTAAACTTAGT+TGG | 0.420459 | 1.3:+34399059 | None:intergenic |
| TGTCGATTCTGCACACCAAC+AGG | 0.457316 | 1.3:+34399347 | None:intergenic |
| ATTAGAATGCCTACTTCTCT+TGG | 0.458025 | 1.3:+34399022 | None:intergenic |
| CCAAATCAAACAAGTTCAAA+AGG | 0.468309 | 1.3:-34398878 | MS.gene069393:CDS |
| AAAAGTTTCACTCACGGTGA+TGG | 0.482608 | 1.3:-34398942 | MS.gene069393:CDS |
| CCCTTGGCAGCTGAAACTTC+AGG | 0.489925 | 1.3:+34399281 | None:intergenic |
| AAAGTTTCACTCACGGTGAT+GGG | 0.500845 | 1.3:-34398941 | MS.gene069393:CDS |
| GCCTGAAGTTTCAGCTGCCA+AGG | 0.504485 | 1.3:-34399282 | MS.gene069393:CDS |
| TCAGATCAGCCAAGAGAAGT+AGG | 0.514953 | 1.3:-34399031 | MS.gene069393:CDS |
| AGGATAAAGCTTCTAATGTA+CGG | 0.527377 | 1.3:-34398911 | MS.gene069393:CDS |
| AAGGTCAGAAGGAAGTATGA+AGG | 0.534360 | 1.3:-34398982 | MS.gene069393:CDS |
| AATTCAAATTTAAGGTCAGA+AGG | 0.550103 | 1.3:-34398993 | MS.gene069393:CDS |
| CCTGAAGTTTCAGCTGCCAA+GGG | 0.551029 | 1.3:-34399281 | MS.gene069393:CDS |
| AGGTCAGAAGGAAGTATGAA+GGG | 0.578400 | 1.3:-34398981 | MS.gene069393:CDS |
| AAAGCTTCTAATGTACGGAT+AGG | 0.583251 | 1.3:-34398906 | MS.gene069393:CDS |
| TCACGGTGATGGGAAGAGAA+AGG | 0.588243 | 1.3:-34398931 | MS.gene069393:CDS |
| GTATGAAGGGCAATAAAAGC+CGG | 0.600813 | 1.3:-34398968 | MS.gene069393:CDS |
| CGGTTCAAAAGTTTCACTCA+CGG | 0.607639 | 1.3:-34398948 | MS.gene069393:CDS |
| TCCTTTGTAGGTCGGAGCGG+AGG | 0.627313 | 1.3:-34399175 | MS.gene069393:intron |
| CACTCCTTTGTAGGTCGGAG+CGG | 0.634577 | 1.3:-34399178 | MS.gene069393:intron |
| GTTGGTGTGCAGAATCGACA+TGG | 0.637497 | 1.3:-34399344 | MS.gene069393:CDS |
| CTGAAGTTTCAGCTGCCAAG+GGG | 0.681640 | 1.3:-34399280 | MS.gene069393:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGATACAAATTCAAATTTA+AGG | - | chr1.3:34399221-34399240 | MS.gene069393:intron | 15.0% |
| ! | TTTCAGCAAAAAAAGAGAAA+GGG | - | chr1.3:34399106-34399125 | MS.gene069393:CDS | 25.0% |
| !! | AATTCAAATTTAAGGTCAGA+AGG | - | chr1.3:34399229-34399248 | MS.gene069393:intron | 25.0% |
| AAACTGAACCAACTAAGTTT+AGG | - | chr1.3:34399155-34399174 | MS.gene069393:CDS | 30.0% | |
| AGGATAAAGCTTCTAATGTA+CGG | - | chr1.3:34399311-34399330 | MS.gene069393:CDS | 30.0% | |
| CCAAATCAAACAAGTTCAAA+AGG | - | chr1.3:34399344-34399363 | MS.gene069393:CDS | 30.0% | |
| GTTTCAGCAAAAAAAGAGAA+AGG | - | chr1.3:34399105-34399124 | MS.gene069393:CDS | 30.0% | |
| TTCAGCAAAAAAAGAGAAAG+GGG | - | chr1.3:34399107-34399126 | MS.gene069393:CDS | 30.0% | |
| !! | CCTTTTGAACTTGTTTGATT+TGG | + | chr1.3:34399347-34399366 | None:intergenic | 30.0% |
| AAAGCTTCTAATGTACGGAT+AGG | - | chr1.3:34399316-34399335 | MS.gene069393:CDS | 35.0% | |
| AGCAATAAAATCATACCCCT+TGG | + | chr1.3:34398960-34398979 | None:intergenic | 35.0% | |
| ATTAGAATGCCTACTTCTCT+TGG | + | chr1.3:34399203-34399222 | None:intergenic | 35.0% | |
| CTGAAACAAGTCTTTCTCTT+GGG | + | chr1.3:34399092-34399111 | None:intergenic | 35.0% | |
| GACAATAGAATTCAGCTTCA+AGG | + | chr1.3:34399017-34399036 | None:intergenic | 35.0% | |
| TCATTGCTCCTAAACTTAGT+TGG | + | chr1.3:34399166-34399185 | None:intergenic | 35.0% | |
| ! | CGCATTTTCTGTATCATTAG+CGG | + | chr1.3:34398921-34398940 | None:intergenic | 35.0% |
| AAAAGTTTCACTCACGGTGA+TGG | - | chr1.3:34399280-34399299 | MS.gene069393:intron | 40.0% | |
| AAAGTTTCACTCACGGTGAT+GGG | - | chr1.3:34399281-34399300 | MS.gene069393:CDS | 40.0% | |
| CGGTTCAAAAGTTTCACTCA+CGG | - | chr1.3:34399274-34399293 | MS.gene069393:intron | 40.0% | |
| GCTGAAACAAGTCTTTCTCT+TGG | + | chr1.3:34399093-34399112 | None:intergenic | 40.0% | |
| GTATGAAGGGCAATAAAAGC+CGG | - | chr1.3:34399254-34399273 | MS.gene069393:intron | 40.0% | |
| TCTTGGGTGTTCGAAATATC+AGG | + | chr1.3:34399076-34399095 | None:intergenic | 40.0% | |
| ! | AAGGTCAGAAGGAAGTATGA+AGG | - | chr1.3:34399240-34399259 | MS.gene069393:intron | 40.0% |
| ! | AGGTCAGAAGGAAGTATGAA+GGG | - | chr1.3:34399241-34399260 | MS.gene069393:intron | 40.0% |
| ! | CGTGAGTGAAACTTTTGAAC+CGG | + | chr1.3:34399276-34399295 | None:intergenic | 40.0% |
| TCAGATCAGCCAAGAGAAGT+AGG | - | chr1.3:34399191-34399210 | MS.gene069393:intron | 45.0% | |
| TCTATCCGTCACTCCTTTGT+AGG | - | chr1.3:34399035-34399054 | MS.gene069393:CDS | 45.0% | |
| CCTGAAGTTTCAGCTGCCAA+GGG | - | chr1.3:34398941-34398960 | MS.gene069393:CDS | 50.0% | |
| CTGAAGTTTCAGCTGCCAAG+GGG | - | chr1.3:34398942-34398961 | MS.gene069393:CDS | 50.0% | |
| GTTGGTGTGCAGAATCGACA+TGG | - | chr1.3:34398878-34398897 | MS.gene069393:CDS | 50.0% | |
| TCCGACCTACAAAGGAGTGA+CGG | + | chr1.3:34399043-34399062 | None:intergenic | 50.0% | |
| TCCGTCACTCCTTTGTAGGT+CGG | - | chr1.3:34399039-34399058 | MS.gene069393:CDS | 50.0% | |
| !! | TCACGGTGATGGGAAGAGAA+AGG | - | chr1.3:34399291-34399310 | MS.gene069393:CDS | 50.0% |
| !! | TGTCGATTCTGCACACCAAC+AGG | + | chr1.3:34398878-34398897 | None:intergenic | 50.0% |
| CACTCCTTTGTAGGTCGGAG+CGG | - | chr1.3:34399044-34399063 | MS.gene069393:CDS | 55.0% | |
| CCCTTGGCAGCTGAAACTTC+AGG | + | chr1.3:34398944-34398963 | None:intergenic | 55.0% | |
| GCCTGAAGTTTCAGCTGCCA+AGG | - | chr1.3:34398940-34398959 | MS.gene069393:CDS | 55.0% | |
| TCCTCCGCTCCGACCTACAA+AGG | + | chr1.3:34399051-34399070 | None:intergenic | 60.0% | |
| TCCTTTGTAGGTCGGAGCGG+AGG | - | chr1.3:34399047-34399066 | MS.gene069393:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 34398872 | 34399372 | 34398872 | ID=MS.gene069393 |
| chr1.3 | mRNA | 34398872 | 34399372 | 34398872 | ID=MS.gene069393.t1;Parent=MS.gene069393 |
| chr1.3 | exon | 34399281 | 34399372 | 34399281 | ID=MS.gene069393.t1.exon1;Parent=MS.gene069393.t1 |
| chr1.3 | CDS | 34399281 | 34399372 | 34399281 | ID=cds.MS.gene069393.t1;Parent=MS.gene069393.t1 |
| chr1.3 | exon | 34398872 | 34399187 | 34398872 | ID=MS.gene069393.t1.exon2;Parent=MS.gene069393.t1 |
| chr1.3 | CDS | 34398872 | 34399187 | 34398872 | ID=cds.MS.gene069393.t1;Parent=MS.gene069393.t1 |
| Gene Sequence |
| Protein sequence |
