Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070624.t1 | XP_003621270.1 | 92.8 | 195 | 10 | 2 | 1 | 195 | 119 | 309 | 7.90E-91 | 343.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070624.t1 | Q9LXF1 | 44.2 | 217 | 90 | 6 | 1 | 195 | 119 | 326 | 3.0e-22 | 106.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070624.t1 | G7L225 | 92.8 | 195 | 10 | 2 | 1 | 195 | 119 | 309 | 5.7e-91 | 343.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049758 | MS.gene070624 | 0.803548 | 3.31E-49 | -1.69E-46 |
| MS.gene051789 | MS.gene070624 | 0.826823 | 2.20E-54 | -1.69E-46 |
| MS.gene052967 | MS.gene070624 | 0.814584 | 1.43E-51 | -1.69E-46 |
| MS.gene053074 | MS.gene070624 | 0.813661 | 2.29E-51 | -1.69E-46 |
| MS.gene054184 | MS.gene070624 | 0.800895 | 1.16E-48 | -1.69E-46 |
| MS.gene054185 | MS.gene070624 | 0.805693 | 1.18E-49 | -1.69E-46 |
| MS.gene055133 | MS.gene070624 | 0.809962 | 1.46E-50 | -1.69E-46 |
| MS.gene055207 | MS.gene070624 | 0.800887 | 1.17E-48 | -1.69E-46 |
| MS.gene056069 | MS.gene070624 | -0.80476 | 1.85E-49 | -1.69E-46 |
| MS.gene057393 | MS.gene070624 | 0.802052 | 6.74E-49 | -1.69E-46 |
| MS.gene059228 | MS.gene070624 | 0.800048 | 1.73E-48 | -1.69E-46 |
| MS.gene060476 | MS.gene070624 | 0.817605 | 3.02E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070624.t1 | MTR_7g011170 | 92.308 | 195 | 11 | 2 | 1 | 195 | 119 | 309 | 6.27e-115 | 329 |
| MS.gene070624.t1 | MTR_6g012690 | 47.082 | 257 | 56 | 11 | 1 | 195 | 119 | 357 | 4.92e-37 | 132 |
| MS.gene070624.t1 | MTR_4g082290 | 63.333 | 60 | 16 | 3 | 1 | 55 | 119 | 177 | 2.21e-13 | 67.8 |
| MS.gene070624.t1 | MTR_4g082230 | 63.333 | 60 | 16 | 3 | 1 | 55 | 119 | 177 | 2.43e-13 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070624.t1 | AT5G15310 | 45.161 | 217 | 88 | 7 | 1 | 195 | 40 | 247 | 7.15e-34 | 121 |
| MS.gene070624.t1 | AT5G15310 | 45.933 | 209 | 87 | 7 | 1 | 195 | 119 | 315 | 4.45e-33 | 120 |
| MS.gene070624.t1 | AT5G15310 | 45.161 | 217 | 88 | 7 | 1 | 195 | 119 | 326 | 9.95e-33 | 120 |
| MS.gene070624.t1 | AT3G01140 | 38.559 | 236 | 95 | 8 | 1 | 195 | 119 | 345 | 5.28e-27 | 105 |
| MS.gene070624.t1 | AT3G01140 | 39.496 | 238 | 90 | 10 | 1 | 195 | 162 | 388 | 1.17e-26 | 105 |
| MS.gene070624.t1 | AT5G15310 | 76.271 | 59 | 12 | 2 | 1 | 58 | 119 | 176 | 4.18e-17 | 77.0 |
Find 33 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTACACTCTCCACCGCTTTC+CGG | 0.265479 | 7.1:+84322267 | None:intergenic |
| GCTAAGATTTGCAGCATTCT+TGG | 0.289751 | 7.1:+84322653 | None:intergenic |
| GTTAGAATTCAAAGATTTGA+TGG | 0.315601 | 7.1:-84322412 | MS.gene070624:CDS |
| ACTTGAAGCTGAAGCAAGAT+TGG | 0.402581 | 7.1:-84322604 | MS.gene070624:CDS |
| AAGAAGAAGGTGAGAAAGAG+TGG | 0.428852 | 7.1:-84322461 | MS.gene070624:CDS |
| ATCTTAGCCACAAGGCTCAA+TGG | 0.433665 | 7.1:-84322638 | MS.gene070624:CDS |
| TCTTAGCCACAAGGCTCAAT+GGG | 0.448848 | 7.1:-84322637 | MS.gene070624:CDS |
| TTTGATGGAGAATTCATCAA+TGG | 0.459211 | 7.1:-84322397 | MS.gene070624:CDS |
| ATGAAGATAACAAGAACTAC+TGG | 0.460330 | 7.1:-84322203 | MS.gene070624:CDS |
| CGCTTTCCGGTGACAAACTC+AGG | 0.463872 | 7.1:+84322280 | None:intergenic |
| CACTTTCCCATTGAGCCTTG+TGG | 0.471844 | 7.1:+84322631 | None:intergenic |
| CAATTGCAACAGCATCAACT+TGG | 0.472770 | 7.1:-84322558 | MS.gene070624:CDS |
| TCATCTTCAAATCAAGTACT+TGG | 0.499747 | 7.1:-84322489 | MS.gene070624:CDS |
| GATTTGCAGCATTCTTGGAA+TGG | 0.519998 | 7.1:+84322658 | None:intergenic |
| GATGGAAGTGGTAGCGGCGG+TGG | 0.528157 | 7.1:-84322237 | MS.gene070624:CDS |
| ACTTGTGATGGAAGTGGTAG+CGG | 0.531173 | 7.1:-84322243 | MS.gene070624:CDS |
| GTACTTGGTATTAAAGAAGA+AGG | 0.544001 | 7.1:-84322474 | MS.gene070624:CDS |
| TAGAACATTGGCGAATCAGA+AGG | 0.556955 | 7.1:+84322150 | None:intergenic |
| TTGCTTGATAATTCTAACTC+CGG | 0.569468 | 7.1:-84322306 | MS.gene070624:CDS |
| ATGATCAGTGTTGCTGAGGA+GGG | 0.575751 | 7.1:-84322342 | MS.gene070624:CDS |
| CGGTGACCTGAGTTTGTCAC+CGG | 0.584452 | 7.1:-84322286 | MS.gene070624:CDS |
| GGAGAGTGTAATACTTGTGA+TGG | 0.589020 | 7.1:-84322255 | MS.gene070624:CDS |
| CGGTGACAAACTCAGGTCAC+CGG | 0.590245 | 7.1:+84322287 | None:intergenic |
| GTAGATGATGAAGGTTGTGA+TGG | 0.594359 | 7.1:+84322516 | None:intergenic |
| GATGATGATGTAGATGATGA+AGG | 0.598426 | 7.1:+84322507 | None:intergenic |
| AATGATCAGTGTTGCTGAGG+AGG | 0.599130 | 7.1:-84322343 | MS.gene070624:CDS |
| GAAGGTGAGAAAGAGTGGAA+AGG | 0.602261 | 7.1:-84322456 | MS.gene070624:CDS |
| TGTAATACTTGTGATGGAAG+TGG | 0.616978 | 7.1:-84322249 | MS.gene070624:CDS |
| CTGAGTTTGTCACCGGAAAG+CGG | 0.631632 | 7.1:-84322279 | MS.gene070624:CDS |
| TGCTGCAAATCTTAGCCACA+AGG | 0.636234 | 7.1:-84322646 | MS.gene070624:CDS |
| GACAATGATCAGTGTTGCTG+AGG | 0.653855 | 7.1:-84322346 | MS.gene070624:CDS |
| AGTTTGTCACCGGAAAGCGG+TGG | 0.707277 | 7.1:-84322276 | MS.gene070624:CDS |
| TGTGATGGAAGTGGTAGCGG+CGG | 0.710359 | 7.1:-84322240 | MS.gene070624:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GTTAGAATTCAAAGATTTGA+TGG | - | chr7.1:84322451-84322470 | MS.gene070624:CDS | 25.0% |
| ATGAAGATAACAAGAACTAC+TGG | - | chr7.1:84322660-84322679 | MS.gene070624:CDS | 30.0% | |
| GTACTTGGTATTAAAGAAGA+AGG | - | chr7.1:84322389-84322408 | MS.gene070624:CDS | 30.0% | |
| TCATCTTCAAATCAAGTACT+TGG | - | chr7.1:84322374-84322393 | MS.gene070624:CDS | 30.0% | |
| TTTGATGGAGAATTCATCAA+TGG | - | chr7.1:84322466-84322485 | MS.gene070624:CDS | 30.0% | |
| ! | TTGCTTGATAATTCTAACTC+CGG | - | chr7.1:84322557-84322576 | MS.gene070624:CDS | 30.0% |
| !!! | GGAAAAAGGGTTTCATTTTT+TGG | + | chr7.1:84322179-84322198 | None:intergenic | 30.0% |
| ! | GATGATGATGTAGATGATGA+AGG | + | chr7.1:84322359-84322378 | None:intergenic | 35.0% |
| ! | TGTAATACTTGTGATGGAAG+TGG | - | chr7.1:84322614-84322633 | MS.gene070624:CDS | 35.0% |
| !! | GGAATGGTTTTCAGGAAAAA+GGG | + | chr7.1:84322192-84322211 | None:intergenic | 35.0% |
| !! | TGGAATGGTTTTCAGGAAAA+AGG | + | chr7.1:84322193-84322212 | None:intergenic | 35.0% |
| !!! | TTTTTTGGCTTGTGTGTAAC+AGG | + | chr7.1:84322164-84322183 | None:intergenic | 35.0% |
| AAGAAGAAGGTGAGAAAGAG+TGG | - | chr7.1:84322402-84322421 | MS.gene070624:CDS | 40.0% | |
| ACTTGAAGCTGAAGCAAGAT+TGG | - | chr7.1:84322259-84322278 | MS.gene070624:CDS | 40.0% | |
| CAATTGCAACAGCATCAACT+TGG | - | chr7.1:84322305-84322324 | MS.gene070624:CDS | 40.0% | |
| GATTTGCAGCATTCTTGGAA+TGG | + | chr7.1:84322208-84322227 | None:intergenic | 40.0% | |
| GCTAAGATTTGCAGCATTCT+TGG | + | chr7.1:84322213-84322232 | None:intergenic | 40.0% | |
| GTAGATGATGAAGGTTGTGA+TGG | + | chr7.1:84322350-84322369 | None:intergenic | 40.0% | |
| TAGAACATTGGCGAATCAGA+AGG | + | chr7.1:84322716-84322735 | None:intergenic | 40.0% | |
| ! | GCATTCTTGGAATGGTTTTC+AGG | + | chr7.1:84322200-84322219 | None:intergenic | 40.0% |
| ! | GGAGAGTGTAATACTTGTGA+TGG | - | chr7.1:84322608-84322627 | MS.gene070624:CDS | 40.0% |
| ATCTTAGCCACAAGGCTCAA+TGG | - | chr7.1:84322225-84322244 | MS.gene070624:CDS | 45.0% | |
| GAAGGTGAGAAAGAGTGGAA+AGG | - | chr7.1:84322407-84322426 | MS.gene070624:CDS | 45.0% | |
| TCTTAGCCACAAGGCTCAAT+GGG | - | chr7.1:84322226-84322245 | MS.gene070624:CDS | 45.0% | |
| TGCTGCAAATCTTAGCCACA+AGG | - | chr7.1:84322217-84322236 | MS.gene070624:CDS | 45.0% | |
| ! | AATGATCAGTGTTGCTGAGG+AGG | - | chr7.1:84322520-84322539 | MS.gene070624:CDS | 45.0% |
| ! | ACTTGTGATGGAAGTGGTAG+CGG | - | chr7.1:84322620-84322639 | MS.gene070624:CDS | 45.0% |
| ! | ATGATCAGTGTTGCTGAGGA+GGG | - | chr7.1:84322521-84322540 | MS.gene070624:CDS | 45.0% |
| ! | GACAATGATCAGTGTTGCTG+AGG | - | chr7.1:84322517-84322536 | MS.gene070624:CDS | 45.0% |
| CACTTTCCCATTGAGCCTTG+TGG | + | chr7.1:84322235-84322254 | None:intergenic | 50.0% | |
| CTGAGTTTGTCACCGGAAAG+CGG | - | chr7.1:84322584-84322603 | MS.gene070624:CDS | 50.0% | |
| TTACACTCTCCACCGCTTTC+CGG | + | chr7.1:84322599-84322618 | None:intergenic | 50.0% | |
| AGTTTGTCACCGGAAAGCGG+TGG | - | chr7.1:84322587-84322606 | MS.gene070624:CDS | 55.0% | |
| CGCTTTCCGGTGACAAACTC+AGG | + | chr7.1:84322586-84322605 | None:intergenic | 55.0% | |
| CGGTGACAAACTCAGGTCAC+CGG | + | chr7.1:84322579-84322598 | None:intergenic | 55.0% | |
| CGGTGACCTGAGTTTGTCAC+CGG | - | chr7.1:84322577-84322596 | MS.gene070624:CDS | 55.0% | |
| !! | TGTGATGGAAGTGGTAGCGG+CGG | - | chr7.1:84322623-84322642 | MS.gene070624:CDS | 55.0% |
| !! | GATGGAAGTGGTAGCGGCGG+TGG | - | chr7.1:84322626-84322645 | MS.gene070624:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 84322149 | 84322736 | 84322149 | ID=MS.gene070624 |
| chr7.1 | mRNA | 84322149 | 84322736 | 84322149 | ID=MS.gene070624.t1;Parent=MS.gene070624 |
| chr7.1 | exon | 84322149 | 84322736 | 84322149 | ID=MS.gene070624.t1.exon1;Parent=MS.gene070624.t1 |
| chr7.1 | CDS | 84322149 | 84322736 | 84322149 | ID=cds.MS.gene070624.t1;Parent=MS.gene070624.t1 |
| Gene Sequence |
| Protein sequence |
