Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052967.t1 | AFK36884.1 | 98.1 | 103 | 2 | 0 | 18 | 120 | 255 | 357 | 1.10E-29 | 139.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052967.t1 | I3S9E2 | 98.1 | 103 | 2 | 0 | 18 | 120 | 255 | 357 | 7.7e-30 | 139.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051626 | MS.gene052967 | 0.857955 | 1.15E-62 | -1.69E-46 |
| MS.gene051789 | MS.gene052967 | 0.857176 | 1.95E-62 | -1.69E-46 |
| MS.gene052967 | MS.gene053074 | 0.855809 | 4.92E-62 | -1.69E-46 |
| MS.gene052967 | MS.gene054184 | 0.829513 | 4.94E-55 | -1.69E-46 |
| MS.gene052967 | MS.gene054185 | 0.833898 | 4.09E-56 | -1.69E-46 |
| MS.gene052967 | MS.gene05456 | 0.808509 | 2.99E-50 | -1.69E-46 |
| MS.gene052967 | MS.gene054596 | 0.830433 | 2.95E-55 | -1.69E-46 |
| MS.gene052967 | MS.gene055133 | 0.816583 | 5.13E-52 | -1.69E-46 |
| MS.gene052967 | MS.gene055207 | 0.825082 | 5.69E-54 | -1.69E-46 |
| MS.gene052967 | MS.gene056380 | 0.811662 | 6.26E-51 | -1.69E-46 |
| MS.gene052967 | MS.gene058168 | 0.81538 | 9.52E-52 | -1.69E-46 |
| MS.gene052967 | MS.gene059006 | 0.83289 | 7.30E-56 | -1.69E-46 |
| MS.gene052967 | MS.gene060476 | 0.813845 | 2.08E-51 | -1.69E-46 |
| MS.gene052967 | MS.gene061362 | 0.828163 | 1.05E-54 | -1.69E-46 |
| MS.gene052967 | MS.gene061893 | 0.814999 | 1.16E-51 | -1.69E-46 |
| MS.gene052967 | MS.gene062354 | 0.81147 | 6.89E-51 | -1.69E-46 |
| MS.gene052967 | MS.gene062429 | 0.804881 | 1.75E-49 | -1.69E-46 |
| MS.gene052967 | MS.gene06339 | 0.826679 | 2.38E-54 | -1.69E-46 |
| MS.gene052967 | MS.gene065378 | 0.80884 | 2.54E-50 | -1.69E-46 |
| MS.gene052967 | MS.gene066856 | 0.826621 | 2.45E-54 | -1.69E-46 |
| MS.gene052967 | MS.gene067305 | 0.818172 | 2.25E-52 | -1.69E-46 |
| MS.gene052967 | MS.gene068614 | 0.803537 | 3.33E-49 | -1.69E-46 |
| MS.gene052967 | MS.gene070594 | 0.801423 | 9.07E-49 | -1.69E-46 |
| MS.gene052967 | MS.gene070624 | 0.814584 | 1.43E-51 | -1.69E-46 |
| MS.gene052967 | MS.gene07942 | 0.843717 | 1.18E-58 | -1.69E-46 |
| MS.gene052967 | MS.gene07948 | 0.817831 | 2.69E-52 | -1.69E-46 |
| MS.gene052967 | MS.gene08514 | 0.817183 | 3.76E-52 | -1.69E-46 |
| MS.gene052967 | MS.gene22587 | 0.816183 | 6.30E-52 | -1.69E-46 |
| MS.gene052967 | MS.gene23012 | 0.863855 | 1.85E-64 | -1.69E-46 |
| MS.gene052967 | MS.gene24039 | 0.855305 | 6.90E-62 | -1.69E-46 |
| MS.gene052967 | MS.gene24114 | 0.816596 | 5.10E-52 | -1.69E-46 |
| MS.gene052967 | MS.gene24546 | 0.868094 | 8.43E-66 | -1.69E-46 |
| MS.gene052967 | MS.gene28341 | 0.853095 | 3.00E-61 | -1.69E-46 |
| MS.gene052967 | MS.gene29146 | 0.835781 | 1.37E-56 | -1.69E-46 |
| MS.gene052967 | MS.gene29822 | 0.832026 | 1.20E-55 | -1.69E-46 |
| MS.gene052967 | MS.gene32978 | 0.856556 | 2.97E-62 | -1.69E-46 |
| MS.gene052967 | MS.gene33382 | 0.808591 | 2.87E-50 | -1.69E-46 |
| MS.gene052967 | MS.gene34106 | 0.808859 | 2.52E-50 | -1.69E-46 |
| MS.gene052967 | MS.gene35428 | 0.843664 | 1.22E-58 | -1.69E-46 |
| MS.gene052967 | MS.gene35590 | 0.831408 | 1.70E-55 | -1.69E-46 |
| MS.gene052967 | MS.gene35592 | 0.832572 | 8.76E-56 | -1.69E-46 |
| MS.gene052967 | MS.gene35695 | 0.868153 | 8.07E-66 | -1.69E-46 |
| MS.gene052967 | MS.gene38528 | 0.835524 | 1.60E-56 | -1.69E-46 |
| MS.gene052967 | MS.gene38772 | 0.800459 | 1.43E-48 | -1.69E-46 |
| MS.gene052967 | MS.gene39496 | 0.801138 | 1.04E-48 | -1.69E-46 |
| MS.gene052967 | MS.gene39658 | 0.800012 | 1.76E-48 | -1.69E-46 |
| MS.gene052967 | MS.gene40950 | 0.804409 | 2.19E-49 | -1.69E-46 |
| MS.gene052967 | MS.gene41839 | 0.81672 | 4.78E-52 | -1.69E-46 |
| MS.gene052967 | MS.gene41915 | 0.826882 | 2.13E-54 | -1.69E-46 |
| MS.gene052967 | MS.gene44012 | 0.809034 | 2.31E-50 | -1.69E-46 |
| MS.gene052967 | MS.gene44274 | 0.821733 | 3.45E-53 | -1.69E-46 |
| MS.gene052967 | MS.gene45085 | 0.855441 | 6.29E-62 | -1.69E-46 |
| MS.gene052967 | MS.gene45439 | 0.81617 | 6.35E-52 | -1.69E-46 |
| MS.gene052967 | MS.gene47283 | 0.806159 | 9.42E-50 | -1.69E-46 |
| MS.gene052967 | MS.gene51304 | 0.801871 | 7.34E-49 | -1.69E-46 |
| MS.gene052967 | MS.gene52365 | 0.803472 | 3.43E-49 | -1.69E-46 |
| MS.gene052967 | MS.gene54407 | 0.81119 | 7.93E-51 | -1.69E-46 |
| MS.gene052967 | MS.gene56979 | 0.807871 | 4.09E-50 | -1.69E-46 |
| MS.gene052967 | MS.gene57222 | 0.86665 | 2.44E-65 | -1.69E-46 |
| MS.gene052967 | MS.gene58532 | 0.813578 | 2.38E-51 | -1.69E-46 |
| MS.gene052967 | MS.gene59372 | 0.818494 | 1.90E-52 | -1.69E-46 |
| MS.gene052967 | MS.gene61586 | 0.80449 | 2.11E-49 | -1.69E-46 |
| MS.gene052967 | MS.gene62594 | 0.803042 | 4.21E-49 | -1.69E-46 |
| MS.gene052967 | MS.gene62720 | 0.884514 | 1.80E-71 | -1.69E-46 |
| MS.gene052967 | MS.gene65399 | 0.824201 | 9.18E-54 | -1.69E-46 |
| MS.gene052967 | MS.gene66736 | 0.813288 | 2.76E-51 | -1.69E-46 |
| MS.gene052967 | MS.gene67384 | 0.825679 | 4.11E-54 | -1.69E-46 |
| MS.gene052967 | MS.gene70814 | 0.80393 | 2.76E-49 | -1.69E-46 |
| MS.gene052967 | MS.gene75321 | 0.84352 | 1.33E-58 | -1.69E-46 |
| MS.gene052967 | MS.gene80842 | 0.807214 | 5.64E-50 | -1.69E-46 |
| MS.gene052967 | MS.gene80996 | 0.811096 | 8.30E-51 | -1.69E-46 |
| MS.gene052967 | MS.gene82214 | 0.81419 | 1.75E-51 | -1.69E-46 |
| MS.gene052967 | MS.gene82810 | 0.826769 | 2.26E-54 | -1.69E-46 |
| MS.gene052967 | MS.gene83190 | 0.801519 | 8.67E-49 | -1.69E-46 |
| MS.gene052967 | MS.gene84833 | 0.813657 | 2.29E-51 | -1.69E-46 |
| MS.gene052967 | MS.gene86114 | 0.842069 | 3.24E-58 | -1.69E-46 |
| MS.gene052967 | MS.gene86236 | 0.808142 | 3.58E-50 | -1.69E-46 |
| MS.gene052967 | MS.gene89326 | 0.805908 | 1.06E-49 | -1.69E-46 |
| MS.gene052967 | MS.gene96503 | 0.817199 | 3.73E-52 | -1.69E-46 |
| MS.gene052967 | MS.gene97857 | 0.821115 | 4.79E-53 | -1.69E-46 |
| MS.gene052967 | MS.gene98316 | 0.802744 | 4.85E-49 | -1.69E-46 |
| MS.gene052967 | MS.gene98318 | 0.836286 | 1.02E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052967.t1 | MTR_6g012690 | 98.333 | 120 | 2 | 0 | 1 | 120 | 238 | 357 | 1.22e-78 | 236 |
| MS.gene052967.t1 | MTR_7g011170 | 54.032 | 124 | 29 | 5 | 13 | 120 | 198 | 309 | 6.41e-23 | 90.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052967.t1 | AT5G15310 | 36.975 | 119 | 54 | 4 | 2 | 120 | 150 | 247 | 8.62e-11 | 57.4 |
| MS.gene052967.t1 | AT5G15310 | 36.975 | 119 | 54 | 4 | 2 | 120 | 229 | 326 | 9.24e-11 | 57.8 |
Find 22 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAGTTAATTCTTCTCCCTC+TGG | 0.417173 | 1.3:-70101762 | MS.gene052967:CDS |
| GGTGATGGTGGAAGTGGAAG+TGG | 0.421766 | 1.3:-70101834 | MS.gene052967:CDS |
| GTGTCTATGATTGAGTTTGT+AGG | 0.434040 | 1.3:-70102092 | None:intergenic |
| TGTCTATGATTGAGTTTGTA+GGG | 0.466901 | 1.3:-70102091 | None:intergenic |
| AGTTCAATTGTGAAAGAAGA+AGG | 0.467308 | 1.3:-70102059 | MS.gene052967:CDS |
| ATGAAGATAACAATAACTAC+TGG | 0.468008 | 1.3:-70101800 | MS.gene052967:CDS |
| AATGGTGGTGATGGTGGAAG+TGG | 0.474351 | 1.3:-70101840 | MS.gene052967:CDS |
| ATGATCAAGATCAAGATCAA+TGG | 0.499871 | 1.3:-70102031 | MS.gene052967:CDS |
| TCATGAGTTGACTAAGTCAA+TGG | 0.505720 | 1.3:-70101967 | MS.gene052967:CDS |
| GATGATGTCATTGCTGAAGA+AGG | 0.549060 | 1.3:-70101933 | MS.gene052967:CDS |
| TCAAAACATTGGTGAACCAG+AGG | 0.555396 | 1.3:+70101746 | None:intergenic |
| ATGATGTCATTGCTGAAGAA+GGG | 0.582083 | 1.3:-70101932 | MS.gene052967:CDS |
| GAATCTAACAATGGTGGTGA+TGG | 0.584861 | 1.3:-70101849 | MS.gene052967:CDS |
| CAAGATCAAGATCAATGGAA+AGG | 0.588024 | 1.3:-70102026 | MS.gene052967:CDS |
| TCTAACAATGGTGGTGATGG+TGG | 0.589722 | 1.3:-70101846 | MS.gene052967:CDS |
| GACTTGAGTTTGTCTGAGAG+TGG | 0.599125 | 1.3:-70101876 | MS.gene052967:CDS |
| CACATCTTATATCAAAACAT+TGG | 0.606562 | 1.3:+70101735 | None:intergenic |
| CAAAACATTGGTGAACCAGA+GGG | 0.631478 | 1.3:+70101747 | None:intergenic |
| TCAATTGTGAAAGAAGAAGG+TGG | 0.631897 | 1.3:-70102056 | MS.gene052967:CDS |
| TTGAGTTTGTCTGAGAGTGG+AGG | 0.701247 | 1.3:-70101873 | MS.gene052967:CDS |
| AGTGGAGGTGAATCTAACAA+TGG | 0.739809 | 1.3:-70101858 | MS.gene052967:CDS |
| GGAGGTGAATCTAACAATGG+TGG | 0.762552 | 1.3:-70101855 | MS.gene052967:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGAAGATAACAATAACTAC+TGG | - | chr1.3:70102032-70102051 | MS.gene052967:CDS | 25.0% |
| AGTTCAATTGTGAAAGAAGA+AGG | - | chr1.3:70101773-70101792 | MS.gene052967:CDS | 30.0% | |
| ATGATCAAGATCAAGATCAA+TGG | - | chr1.3:70101801-70101820 | MS.gene052967:CDS | 30.0% | |
| ATGATGTCATTGCTGAAGAA+GGG | - | chr1.3:70101900-70101919 | MS.gene052967:CDS | 35.0% | |
| CAAGATCAAGATCAATGGAA+AGG | - | chr1.3:70101806-70101825 | MS.gene052967:CDS | 35.0% | |
| TCAATTGTGAAAGAAGAAGG+TGG | - | chr1.3:70101776-70101795 | MS.gene052967:CDS | 35.0% | |
| TTAGTTAATTCTTCTCCCTC+TGG | - | chr1.3:70102070-70102089 | MS.gene052967:CDS | 35.0% | |
| ! | TCATGAGTTGACTAAGTCAA+TGG | - | chr1.3:70101865-70101884 | MS.gene052967:CDS | 35.0% |
| AGTGGAGGTGAATCTAACAA+TGG | - | chr1.3:70101974-70101993 | MS.gene052967:CDS | 40.0% | |
| CAAAACATTGGTGAACCAGA+GGG | + | chr1.3:70102088-70102107 | None:intergenic | 40.0% | |
| GATGATGTCATTGCTGAAGA+AGG | - | chr1.3:70101899-70101918 | MS.gene052967:CDS | 40.0% | |
| TCAAAACATTGGTGAACCAG+AGG | + | chr1.3:70102089-70102108 | None:intergenic | 40.0% | |
| ! | GAATCTAACAATGGTGGTGA+TGG | - | chr1.3:70101983-70102002 | MS.gene052967:CDS | 40.0% |
| GACTTGAGTTTGTCTGAGAG+TGG | - | chr1.3:70101956-70101975 | MS.gene052967:CDS | 45.0% | |
| GGAGGTGAATCTAACAATGG+TGG | - | chr1.3:70101977-70101996 | MS.gene052967:CDS | 45.0% | |
| TTGAGTTTGTCTGAGAGTGG+AGG | - | chr1.3:70101959-70101978 | MS.gene052967:CDS | 45.0% | |
| !! | TCTAACAATGGTGGTGATGG+TGG | - | chr1.3:70101986-70102005 | MS.gene052967:CDS | 45.0% |
| !! | AATGGTGGTGATGGTGGAAG+TGG | - | chr1.3:70101992-70102011 | MS.gene052967:CDS | 50.0% |
| !! | GGTGATGGTGGAAGTGGAAG+TGG | - | chr1.3:70101998-70102017 | MS.gene052967:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 70101746 | 70102108 | 70101746 | ID=MS.gene052967 |
| chr1.3 | mRNA | 70101746 | 70102108 | 70101746 | ID=MS.gene052967.t1;Parent=MS.gene052967 |
| chr1.3 | exon | 70101746 | 70102108 | 70101746 | ID=MS.gene052967.t1.exon1;Parent=MS.gene052967.t1 |
| chr1.3 | CDS | 70101746 | 70102108 | 70101746 | ID=cds.MS.gene052967.t1;Parent=MS.gene052967.t1 |
| Gene Sequence |
| Protein sequence |
