AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene071404

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071404.t1 MTR_2g015500 85.232 237 35 0 1 237 1 237 6.61e-155 430
MS.gene071404.t1 MTR_2g015510 81.356 236 42 1 2 237 3 236 2.95e-145 405
MS.gene071404.t1 MTR_2g015480 73.246 228 42 5 12 237 65 275 5.24e-108 313
MS.gene071404.t1 MTR_4g105680 46.377 207 102 3 25 228 51 251 3.16e-55 178
MS.gene071404.t1 MTR_7g068530 37.736 212 122 2 4 215 6 207 1.74e-50 165
MS.gene071404.t1 MTR_4g016510 37.264 212 125 3 4 215 6 209 1.43e-49 163
MS.gene071404.t1 MTR_7g068550 38.208 212 121 3 4 215 6 207 1.96e-49 162
MS.gene071404.t1 MTR_7g068460 36.449 214 126 3 2 215 31 234 1.52e-46 156
MS.gene071404.t1 MTR_3g070460 35.981 214 125 3 4 215 6 209 8.38e-45 150
MS.gene071404.t1 MTR_7g068500 36.279 215 125 4 2 215 30 233 2.27e-44 150
MS.gene071404.t1 MTR_7g068470 33.962 212 130 4 4 215 2 203 3.98e-41 141
MS.gene071404.t1 MTR_7g068470 34.434 212 125 5 4 215 2 199 1.17e-40 140
MS.gene071404.t1 MTR_7g068450 33.491 212 135 2 4 215 2 207 3.83e-38 134
MS.gene071404.t1 MTR_4g016500 30.137 146 64 3 33 178 277 384 5.85e-17 79.7
MS.gene071404.t1 MTR_7g068520 47.458 59 30 1 4 62 3 60 6.38e-13 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071404.t1 AT5G45920 67.797 236 69 2 4 237 3 233 6.16e-117 334
MS.gene071404.t1 AT5G45920 67.797 236 69 2 4 237 3 233 6.16e-117 334
MS.gene071404.t1 AT5G45920 70.968 186 47 2 4 187 3 183 9.86e-96 278
MS.gene071404.t1 AT5G45920 70.968 186 47 2 4 187 3 183 9.86e-96 278
MS.gene071404.t1 AT5G62930 52.632 228 99 2 4 228 2 223 3.06e-78 236
MS.gene071404.t1 AT5G62930 52.632 228 99 2 4 228 28 249 1.14e-77 235
MS.gene071404.t1 AT5G62930 50.000 164 79 1 68 228 2 165 1.96e-49 160
MS.gene071404.t1 AT2G38180 38.389 211 120 3 4 214 6 206 6.96e-46 155
MS.gene071404.t1 AT3G11210 33.750 240 140 5 4 237 6 232 7.39e-45 151

Find 64 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAGCTTCATTTGTCCTTTC+AGG 0.154934 2.4:+69545967 None:intergenic
TTGGATCATTATCAGCTAAA+AGG 0.222600 2.4:+69545472 None:intergenic
AAGTCGTGCTTCTTCATCTA+TGG 0.253796 2.4:+69546345 None:intergenic
AAATACCTTTCATTGATCTT+TGG 0.264004 2.4:-69545900 MS.gene071404:CDS
AAATGCCTTCAGTGATCATT+TGG 0.347943 2.4:+69545453 None:intergenic
TCCTTTGGTGTTGGAGGATG+GGG 0.361517 2.4:-69548687 MS.gene071404:CDS
TCGTCACCGCCATGTGATAC+TGG 0.369729 2.4:+69547192 None:intergenic
CAAAGCAACAGGAGCAGTTT+CGG 0.379507 2.4:+69547164 None:intergenic
GAAAGGTATTTGACACTCAT+TGG 0.380698 2.4:+69545912 None:intergenic
TGGATCATTATCAGCTAAAA+GGG 0.398671 2.4:+69545473 None:intergenic
CTATGGGAGGAGGAGTTATA+AGG 0.399420 2.4:+69546362 None:intergenic
GACGGTTTGCATCTCACTAA+TGG 0.400983 2.4:-69545579 MS.gene071404:CDS
TCGATCACTGAAGAATCCTT+TGG 0.438191 2.4:-69548702 MS.gene071404:CDS
AGGGAATCAATTTGTGTTTG+AGG 0.442405 2.4:-69545556 MS.gene071404:CDS
ACTAAAATGCAACGGTTCCC+TGG 0.444415 2.4:-69545877 MS.gene071404:CDS
AAGAAAACTGTCAAAGCAAC+AGG 0.453323 2.4:+69547153 None:intergenic
AATCCTTTGGTGTTGGAGGA+TGG 0.456779 2.4:-69548689 MS.gene071404:CDS
ATCCTTTGGTGTTGGAGGAT+GGG 0.456978 2.4:-69548688 MS.gene071404:CDS
GTCCTTTCAGGAAGCCCCTC+CGG 0.465657 2.4:+69545979 None:intergenic
AAATGCAACGGTTCCCTGGA+TGG 0.484613 2.4:-69545873 MS.gene071404:CDS
GGCACTGAAGGTGTTGGAAA+GGG 0.491788 2.4:-69547220 MS.gene071404:CDS
TATAACAAAACATGACTAAG+AGG 0.493140 2.4:-69548749 None:intergenic
ACTGAAGAATCCTTTGGTGT+TGG 0.507462 2.4:-69548696 MS.gene071404:CDS
TAGATGAAGAAGCACGACTT+AGG 0.509915 2.4:-69546342 MS.gene071404:intron
GAAGAATCCTTTGGTGTTGG+AGG 0.526860 2.4:-69548693 MS.gene071404:CDS
TGCCAACTATTTCTCTCGTA+CGG 0.529977 2.4:-69548655 MS.gene071404:intron
ATCCGGAGGGGCTTCCTGAA+AGG 0.532035 2.4:-69545981 MS.gene071404:CDS
TTATTTGTTTGTGAACAATC+CGG 0.532846 2.4:-69545998 MS.gene071404:CDS
TCACCGAAAAGATATATTTG+TGG 0.535472 2.4:+69548726 None:intergenic
AGTCGTGCTTCTTCATCTAT+GGG 0.542373 2.4:+69546346 None:intergenic
AATGCAACGGTTCCCTGGAT+GGG 0.542822 2.4:-69545872 MS.gene071404:CDS
GAAAGGACAAATGAAGCTGC+TGG 0.551991 2.4:-69545964 MS.gene071404:CDS
ATCTTTGGACTAAAATGCAA+CGG 0.555620 2.4:-69545885 MS.gene071404:CDS
TACCGTACGAGAGAAATAGT+TGG 0.562274 2.4:+69548653 None:intergenic
GGGCACTGAAGGTGTTGGAA+AGG 0.563729 2.4:-69547221 MS.gene071404:CDS
ATAAGGACAACATTAGTTGA+CGG 0.572638 2.4:+69546379 None:intergenic
AGTATCACATGGCGGTGACG+AGG 0.572977 2.4:-69547190 MS.gene071404:CDS
GCTTCTTCATCTATGGGAGG+AGG 0.576690 2.4:+69546352 None:intergenic
TGTGAGTCTAGAATCTATGC+AGG 0.579365 2.4:-69545505 MS.gene071404:CDS
TGTTCCTCTTCATGAGTACA+AGG 0.584094 2.4:-69547082 MS.gene071404:CDS
ATGATCCAAATGATCACTGA+AGG 0.587832 2.4:-69545458 None:intergenic
AAAAGGAGACAATGGAGCGA+AGG 0.597580 2.4:+69547055 None:intergenic
ACCCCATCCTCCAACACCAA+AGG 0.599552 2.4:+69548686 None:intergenic
TGGTGTTCAGAGGGTATCGT+GGG 0.600203 2.4:-69547257 MS.gene071404:CDS
TTAGTCCAAAGATCAATGAA+AGG 0.601257 2.4:+69545895 None:intergenic
GGTTTGCATCTCACTAATGG+AGG 0.604059 2.4:-69545576 MS.gene071404:CDS
TTGTCCTTGTACTCATGAAG+AGG 0.610871 2.4:+69547078 None:intergenic
TAACACACGATGGGCACTGA+AGG 0.617976 2.4:-69547232 MS.gene071404:CDS
AGGCTGATGTGGTGTTCAGA+GGG 0.618365 2.4:-69547266 MS.gene071404:CDS
GGATCATTATCAGCTAAAAG+GGG 0.619821 2.4:+69545474 None:intergenic
ACGATGGGCACTGAAGGTGT+TGG 0.625863 2.4:-69547226 MS.gene071404:CDS
GTGGTGTTCAGAGGGTATCG+TGG 0.629790 2.4:-69547258 MS.gene071404:CDS
TGCTAAAGCATGCATTTCTG+TGG 0.632567 2.4:-69545935 MS.gene071404:CDS
TAGGCTGATGTGGTGTTCAG+AGG 0.635589 2.4:-69547267 MS.gene071404:CDS
TTGTTTGTGAACAATCCGGA+GGG 0.641948 2.4:-69545994 MS.gene071404:CDS
TCGTGGGTATAACACACGAT+GGG 0.646142 2.4:-69547241 MS.gene071404:CDS
TGTTTGTGAACAATCCGGAG+GGG 0.648290 2.4:-69545993 MS.gene071404:CDS
GTATCACATGGCGGTGACGA+GGG 0.648679 2.4:-69547189 MS.gene071404:CDS
GATCATTATCAGCTAAAAGG+GGG 0.651240 2.4:+69545475 None:intergenic
CGTGCTTCTTCATCTATGGG+AGG 0.659087 2.4:+69546349 None:intergenic
TTTGTTTGTGAACAATCCGG+AGG 0.659446 2.4:-69545995 MS.gene071404:CDS
GTTTGCATCTCACTAATGGA+GGG 0.661505 2.4:-69545575 MS.gene071404:CDS
ATCGTGGGTATAACACACGA+TGG 0.679127 2.4:-69547242 MS.gene071404:CDS
GCTCGTGTGATAGGCTGATG+TGG 0.704764 2.4:-69547277 MS.gene071404:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAATAAAATAAATAAA+AGG - chr2.4:69546488-69546507 MS.gene071404:intron 0.0%
!!! AATTAAAAAAAAATTATTTT+TGG + chr2.4:69546772-69546791 None:intergenic 0.0%
!! GTATAAATAAATCATATAAT+TGG - chr2.4:69547761-69547780 MS.gene071404:intron 10.0%
!!! AATAAATAAAAGGTTAATTT+TGG - chr2.4:69546498-69546517 MS.gene071404:intron 10.0%
!!! CATTAGTATTTTAAATTAAA+TGG + chr2.4:69548439-69548458 None:intergenic 10.0%
!!! CTTTAAAATGATTTTTTTTT+TGG - chr2.4:69546191-69546210 MS.gene071404:intron 10.0%
!!! TTATTAAAATTAAAAGTGTT+TGG - chr2.4:69546023-69546042 MS.gene071404:intron 10.0%
!! AAAATTCTAAACATTTAAAG+TGG + chr2.4:69547695-69547714 None:intergenic 15.0%
!! ATAAATATAAATGACGTAAA+AGG - chr2.4:69546070-69546089 MS.gene071404:intron 15.0%
!! ATATGATTTATTTATACAAG+AGG + chr2.4:69547758-69547777 None:intergenic 15.0%
!! ATTAAATATGCAAAGATAAT+TGG - chr2.4:69548484-69548503 MS.gene071404:intron 15.0%
!! TATTATATGAATTATGAACA+AGG - chr2.4:69546342-69546361 MS.gene071404:intron 15.0%
!!! AAAAATCTTATTTTAAAAGC+CGG + chr2.4:69547272-69547291 None:intergenic 15.0%
!!! AATGTTTTTATTTTTCGTTA+TGG - chr2.4:69545693-69545712 MS.gene071404:intron 15.0%
!!! ACATTTGATTTATCATATAA+TGG + chr2.4:69547534-69547553 None:intergenic 15.0%
!!! CTTAATTTTGTTTTTTCTTT+AGG - chr2.4:69547610-69547629 MS.gene071404:intron 15.0%
!! AAATCAAATGTAGAAATTGT+AGG - chr2.4:69547543-69547562 MS.gene071404:intron 20.0%
!! AACTTATTAATTAGTGAGTT+GGG - chr2.4:69547411-69547430 MS.gene071404:intron 20.0%
!! AAGAAAACCTAATAAAAACA+AGG + chr2.4:69548109-69548128 None:intergenic 20.0%
!! AGAAAACCTAATAAAAACAA+GGG + chr2.4:69548108-69548127 None:intergenic 20.0%
!! ATAAAAAAACTAAGAGATGA+AGG - chr2.4:69548671-69548690 MS.gene071404:CDS 20.0%
!! ATTTCAATCTATTACAAATC+TGG + chr2.4:69545647-69545666 None:intergenic 20.0%
!! TAAAAACAATGACAAAATCA+AGG + chr2.4:69547904-69547923 None:intergenic 20.0%
!! TAAAATACTAATGAACATGA+TGG - chr2.4:69548446-69548465 MS.gene071404:intron 20.0%
!! TAAACATAAATGAAGAGTTA+AGG - chr2.4:69547243-69547262 MS.gene071404:CDS 20.0%
!! TAATAAATAAACCCGTTATT+AGG + chr2.4:69547320-69547339 None:intergenic 20.0%
!! TATAGAAGTTAATCATACTT+AGG - chr2.4:69548158-69548177 MS.gene071404:intron 20.0%
!!! AACAAAGAAGGTAAAATTTT+TGG + chr2.4:69548373-69548392 None:intergenic 20.0%
!!! AAGAAAATGTTTCCATTTTT+GGG + chr2.4:69547968-69547987 None:intergenic 20.0%
!!! TAAGAAAATGTTTCCATTTT+TGG + chr2.4:69547969-69547988 None:intergenic 20.0%
!!! TACTTTCTCAAAAATCATTT+TGG + chr2.4:69546843-69546862 None:intergenic 20.0%
!!! TGATTTTGTCATTGTTTTTA+GGG - chr2.4:69547904-69547923 MS.gene071404:intron 20.0%
!!! TTGATTTTGTCATTGTTTTT+AGG - chr2.4:69547903-69547922 MS.gene071404:intron 20.0%
!!! TTTGTTTTTTCTTTAGGTTT+AGG - chr2.4:69547616-69547635 MS.gene071404:intron 20.0%
!!! TTTTGGAAAACATCTTTAAA+AGG - chr2.4:69546208-69546227 MS.gene071404:intron 20.0%
! AAAACATGTGAAACAGATAT+AGG + chr2.4:69546700-69546719 None:intergenic 25.0%
! AAATACCTTTCATTGATCTT+TGG - chr2.4:69548299-69548318 MS.gene071404:intron 25.0%
! AGAAAAAACATGAAATGACA+TGG + chr2.4:69545608-69545627 None:intergenic 25.0%
! AGTGAACAAGAAATCTTTAA+AGG - chr2.4:69546381-69546400 MS.gene071404:CDS 25.0%
! AGTTAATGAAACCTAATAAC+GGG - chr2.4:69547306-69547325 MS.gene071404:intron 25.0%
! CACTATGGAAAATATTTGTT+AGG - chr2.4:69545865-69545884 MS.gene071404:CDS 25.0%
! GAAATGTGCAAATTTGATAT+TGG - chr2.4:69547388-69547407 MS.gene071404:intron 25.0%
! GAACTTATTAATTAGTGAGT+TGG - chr2.4:69547410-69547429 MS.gene071404:intron 25.0%
! GAGTTAATGAAACCTAATAA+CGG - chr2.4:69547305-69547324 MS.gene071404:intron 25.0%
! TAACAATGACTAATCTATGT+TGG + chr2.4:69546723-69546742 None:intergenic 25.0%
! TAAGAGAAGATAAACAAAGA+AGG + chr2.4:69548385-69548404 None:intergenic 25.0%
! TATCTTCTCTTACAATACTT+TGG - chr2.4:69548393-69548412 MS.gene071404:intron 25.0%
! TTACCAAGTTACCTTAAAAA+AGG + chr2.4:69547164-69547183 None:intergenic 25.0%
! TTATTTGTTTGTGAACAATC+CGG - chr2.4:69548201-69548220 MS.gene071404:intron 25.0%
!! ATGCTATGGTATTATAGTTT+TGG - chr2.4:69548563-69548582 MS.gene071404:intron 25.0%
!! GGTGACATTTTACATTTTAA+TGG + chr2.4:69547513-69547532 None:intergenic 25.0%
!! TCATCAAAATTGACTTTGTA+TGG + chr2.4:69548521-69548540 None:intergenic 25.0%
!!! ACATTTCCCTTGTTTTTATT+AGG - chr2.4:69548099-69548118 MS.gene071404:intron 25.0%
!!! CTTTGAGTTTTTCATTTTAG+AGG + chr2.4:69547445-69547464 None:intergenic 25.0%
!!! TCAATTTTTAGTTCACACTA+TGG - chr2.4:69545850-69545869 MS.gene071404:intron 25.0%
!!! TTTCATGTTTTTTCTCTTGA+TGG - chr2.4:69545613-69545632 MS.gene071404:intron 25.0%
AAAAAACAGCAACATTCTTG+TGG + chr2.4:69546456-69546475 None:intergenic 30.0%
AAGAGGCTCAAATATCTTAT+CGG + chr2.4:69545812-69545831 None:intergenic 30.0%
AATCAAAGTATGTGATGCTA+TGG - chr2.4:69548549-69548568 MS.gene071404:intron 30.0%
ATAAGGACAACATTAGTTGA+CGG + chr2.4:69547823-69547842 None:intergenic 30.0%
ATCTTTGGACTAAAATGCAA+CGG - chr2.4:69548314-69548333 MS.gene071404:intron 30.0%
ATTTGAGCCTCTTAAATATG+TGG - chr2.4:69545819-69545838 MS.gene071404:intron 30.0%
TACCTTAAAAAAGGAGACAA+TGG + chr2.4:69547155-69547174 None:intergenic 30.0%
TCACCGAAAAGATATATTTG+TGG + chr2.4:69545476-69545495 None:intergenic 30.0%
TGGATCATTATCAGCTAAAA+GGG + chr2.4:69548729-69548748 None:intergenic 30.0%
TGTTAACTTAAATGCAGTGA+CGG - chr2.4:69548602-69548621 MS.gene071404:intron 30.0%
TTAGTCCAAAGATCAATGAA+AGG + chr2.4:69548307-69548326 None:intergenic 30.0%
TTGATAGAATAAGACGTGTT+TGG + chr2.4:69546616-69546635 None:intergenic 30.0%
TTGGATCATTATCAGCTAAA+AGG + chr2.4:69548730-69548749 None:intergenic 30.0%
! AGGCCACAAATATATCTTTT+CGG - chr2.4:69545470-69545489 MS.gene071404:CDS 30.0%
! TGAAAAACAGAGCATCTATT+AGG + chr2.4:69547094-69547113 None:intergenic 30.0%
! TTGCTTTGACAGTTTTCTTT+GGG - chr2.4:69547050-69547069 MS.gene071404:CDS 30.0%
!!! TCTCCTTTTTTAAGGTAACT+TGG - chr2.4:69547158-69547177 MS.gene071404:CDS 30.0%
AAACAGATATAGGCTGTGTT+TGG + chr2.4:69546690-69546709 None:intergenic 35.0%
AAATGAAGAGTTAAGGAGAC+CGG - chr2.4:69547250-69547269 MS.gene071404:CDS 35.0%
AAATGACATGGAAACATGGT+TGG + chr2.4:69545596-69545615 None:intergenic 35.0%
AACCGTAATATATGACAACC+TGG - chr2.4:69546405-69546424 MS.gene071404:intron 35.0%
AAGAAAACTGTCAAAGCAAC+AGG + chr2.4:69547049-69547068 None:intergenic 35.0%
ACCCAGGTTGTCATATATTA+CGG + chr2.4:69546410-69546429 None:intergenic 35.0%
ACCGTAATATATGACAACCT+GGG - chr2.4:69546406-69546425 MS.gene071404:intron 35.0%
AGAGGCTCAAATATCTTATC+GGG + chr2.4:69545811-69545830 None:intergenic 35.0%
AGGGAATCAATTTGTGTTTG+AGG - chr2.4:69548643-69548662 MS.gene071404:intron 35.0%
ATATATGACAACCTGGGTTA+TGG - chr2.4:69546412-69546431 MS.gene071404:intron 35.0%
CAAAAAACGCTATAAGCTAG+TGG - chr2.4:69546577-69546596 MS.gene071404:intron 35.0%
CATGAAATGACATGGAAACA+TGG + chr2.4:69545600-69545619 None:intergenic 35.0%
CATGTTAAGCAACCCAAAAA+TGG - chr2.4:69547953-69547972 MS.gene071404:intron 35.0%
CTCTTGACCACATATTTAAG+AGG + chr2.4:69545829-69545848 None:intergenic 35.0%
GATCATTATCAGCTAAAAGG+GGG + chr2.4:69548727-69548746 None:intergenic 35.0%
GATGGGAAAAAGTTTACTTG+AGG - chr2.4:69548344-69548363 MS.gene071404:intron 35.0%
GGATCATTATCAGCTAAAAG+GGG + chr2.4:69548728-69548747 None:intergenic 35.0%
TATATGACAACCTGGGTTAT+GGG - chr2.4:69546413-69546432 MS.gene071404:intron 35.0%
TTGCTTAACATGTGCCTTAA+GGG + chr2.4:69547945-69547964 None:intergenic 35.0%
! CTTTTGAATTTCAGAAGCGA+TGG - chr2.4:69547797-69547816 MS.gene071404:intron 35.0%
! TAAGCTAGCTTATAGCTTGT+TGG + chr2.4:69546658-69546677 None:intergenic 35.0%
! TGCTTTGACAGTTTTCTTTG+GGG - chr2.4:69547051-69547070 MS.gene071404:CDS 35.0%
!! AAGTAAACTTTTTCCCATCC+AGG + chr2.4:69548343-69548362 None:intergenic 35.0%
!! AATCATTAACTTGTGCCTTG+AGG - chr2.4:69548066-69548085 MS.gene071404:intron 35.0%
!! AGTAAACTTTTTCCCATCCA+GGG + chr2.4:69548342-69548361 None:intergenic 35.0%
!! ATCATTAACTTGTGCCTTGA+GGG - chr2.4:69548067-69548086 MS.gene071404:intron 35.0%
!! GAAAGGTATTTGACACTCAT+TGG + chr2.4:69548290-69548309 None:intergenic 35.0%
!! GTTGCTTTGACAGTTTTCTT+TGG - chr2.4:69547049-69547068 MS.gene071404:intron 35.0%
!!! CTCCATTGTCTCCTTTTTTA+AGG - chr2.4:69547150-69547169 MS.gene071404:CDS 35.0%
AAGTCGTGCTTCTTCATCTA+TGG + chr2.4:69547857-69547876 None:intergenic 40.0%
AGTCGTGCTTCTTCATCTAT+GGG + chr2.4:69547856-69547875 None:intergenic 40.0%
ATCAAATGAGCTCGTGTGAT+AGG - chr2.4:69546913-69546932 MS.gene071404:intron 40.0%
GTTGCTTAACATGTGCCTTA+AGG + chr2.4:69547946-69547965 None:intergenic 40.0%
GTTTGCATCTCACTAATGGA+GGG - chr2.4:69548624-69548643 MS.gene071404:intron 40.0%
TACCGTACGAGAGAAATAGT+TGG + chr2.4:69545549-69545568 None:intergenic 40.0%
TAGGAGAGAGAATCCACTAT+GGG - chr2.4:69545884-69545903 MS.gene071404:CDS 40.0%
TCGATCACTGAAGAATCCTT+TGG - chr2.4:69545497-69545516 MS.gene071404:CDS 40.0%
TGCTAAAGCATGCATTTCTG+TGG - chr2.4:69548264-69548283 MS.gene071404:intron 40.0%
TGTTCCTCTTCATGAGTACA+AGG - chr2.4:69547117-69547136 MS.gene071404:CDS 40.0%
TTAGGAGAGAGAATCCACTA+TGG - chr2.4:69545883-69545902 MS.gene071404:CDS 40.0%
TTGTCCTTGTACTCATGAAG+AGG + chr2.4:69547124-69547143 None:intergenic 40.0%
TTGTTTGTGAACAATCCGGA+GGG - chr2.4:69548205-69548224 MS.gene071404:intron 40.0%
TTTACCAAACACACCCATAG+TGG + chr2.4:69545900-69545919 None:intergenic 40.0%
TTTGTTTGTGAACAATCCGG+AGG - chr2.4:69548204-69548223 MS.gene071404:intron 40.0%
! ACTGAAGAATCCTTTGGTGT+TGG - chr2.4:69545503-69545522 MS.gene071404:CDS 40.0%
! AGGGTTTTTCCAGTATCACA+TGG - chr2.4:69546998-69547017 MS.gene071404:intron 40.0%
! GTTTTTCCAGTATCACATGG+CGG - chr2.4:69547001-69547020 MS.gene071404:intron 40.0%
! TAGATGAAGAAGCACGACTT+AGG - chr2.4:69547857-69547876 MS.gene071404:intron 40.0%
! TGCCAACTATTTCTCTCGTA+CGG - chr2.4:69545544-69545563 MS.gene071404:CDS 40.0%
! TGTGAGTCTAGAATCTATGC+AGG - chr2.4:69548694-69548713 MS.gene071404:CDS 40.0%
!! AATGTTAACTTGTGCCCTCA+AGG + chr2.4:69548084-69548103 None:intergenic 40.0%
!! CATGTTAACTTGTGCCCTTA+AGG - chr2.4:69547928-69547947 MS.gene071404:intron 40.0%
AAAAGGAGACAATGGAGCGA+AGG + chr2.4:69547147-69547166 None:intergenic 45.0%
ACTAAAATGCAACGGTTCCC+TGG - chr2.4:69548322-69548341 MS.gene071404:intron 45.0%
ATCGTGGGTATAACACACGA+TGG - chr2.4:69546957-69546976 MS.gene071404:intron 45.0%
CAAAGCAACAGGAGCAGTTT+CGG + chr2.4:69547038-69547057 None:intergenic 45.0%
CTATGGGAGGAGGAGTTATA+AGG + chr2.4:69547840-69547859 None:intergenic 45.0%
GAAAGGACAAATGAAGCTGC+TGG - chr2.4:69548235-69548254 MS.gene071404:intron 45.0%
GAATCCACTATGGGTGTGTT+TGG - chr2.4:69545893-69545912 MS.gene071404:CDS 45.0%
GACGGTTTGCATCTCACTAA+TGG - chr2.4:69548620-69548639 MS.gene071404:intron 45.0%
GCAGCTTCATTTGTCCTTTC+AGG + chr2.4:69548235-69548254 None:intergenic 45.0%
GGTTTGCATCTCACTAATGG+AGG - chr2.4:69548623-69548642 MS.gene071404:intron 45.0%
TCGTGGGTATAACACACGAT+GGG - chr2.4:69546958-69546977 MS.gene071404:intron 45.0%
TGTTTGTGAACAATCCGGAG+GGG - chr2.4:69548206-69548225 MS.gene071404:intron 45.0%
!! AATCCTTTGGTGTTGGAGGA+TGG - chr2.4:69545510-69545529 MS.gene071404:CDS 45.0%
!! ATCCTTTGGTGTTGGAGGAT+GGG - chr2.4:69545511-69545530 MS.gene071404:CDS 45.0%
!! GAAGAATCCTTTGGTGTTGG+AGG - chr2.4:69545506-69545525 MS.gene071404:CDS 45.0%
!!! TATTTTTTAATTTCAATTAT+AGG - chr2.4:69547732-69547751 MS.gene071404:intron 5.0%
AAATGCAACGGTTCCCTGGA+TGG - chr2.4:69548326-69548345 MS.gene071404:intron 50.0%
AATGCAACGGTTCCCTGGAT+GGG - chr2.4:69548327-69548346 MS.gene071404:intron 50.0%
ACCTGGGTTATGGGTAAACG+TGG - chr2.4:69546422-69546441 MS.gene071404:intron 50.0%
AGGCTGATGTGGTGTTCAGA+GGG - chr2.4:69546933-69546952 MS.gene071404:intron 50.0%
CGTGCTTCTTCATCTATGGG+AGG + chr2.4:69547853-69547872 None:intergenic 50.0%
GCTTCTTCATCTATGGGAGG+AGG + chr2.4:69547850-69547869 None:intergenic 50.0%
TAGGCTGATGTGGTGTTCAG+AGG - chr2.4:69546932-69546951 MS.gene071404:intron 50.0%
TCCACGTTTACCCATAACCC+AGG + chr2.4:69546426-69546445 None:intergenic 50.0%
!! GGCACTGAAGGTGTTGGAAA+GGG - chr2.4:69546979-69546998 MS.gene071404:intron 50.0%
!! TAACACACGATGGGCACTGA+AGG - chr2.4:69546967-69546986 MS.gene071404:intron 50.0%
!! TCCTTTGGTGTTGGAGGATG+GGG - chr2.4:69545512-69545531 MS.gene071404:CDS 50.0%
!! TGGTGTTCAGAGGGTATCGT+GGG - chr2.4:69546942-69546961 MS.gene071404:intron 50.0%
ACCCCATCCTCCAACACCAA+AGG + chr2.4:69545516-69545535 None:intergenic 55.0%
AGTATCACATGGCGGTGACG+AGG - chr2.4:69547009-69547028 MS.gene071404:intron 55.0%
GCTCGTGTGATAGGCTGATG+TGG - chr2.4:69546922-69546941 MS.gene071404:intron 55.0%
GTATCACATGGCGGTGACGA+GGG - chr2.4:69547010-69547029 MS.gene071404:intron 55.0%
TCGTCACCGCCATGTGATAC+TGG + chr2.4:69547010-69547029 None:intergenic 55.0%
!! ACGATGGGCACTGAAGGTGT+TGG - chr2.4:69546973-69546992 MS.gene071404:intron 55.0%
!! GGGCACTGAAGGTGTTGGAA+AGG - chr2.4:69546978-69546997 MS.gene071404:intron 55.0%
!! GTGGTGTTCAGAGGGTATCG+TGG - chr2.4:69546941-69546960 MS.gene071404:intron 55.0%
ATCCGGAGGGGCTTCCTGAA+AGG - chr2.4:69548218-69548237 MS.gene071404:intron 60.0%
GTCCTTTCAGGAAGCCCCTC+CGG + chr2.4:69548223-69548242 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 69545461 69548760 69545461 ID=MS.gene071404
chr2.4 mRNA 69545461 69548760 69545461 ID=MS.gene071404.t1;Parent=MS.gene071404
chr2.4 exon 69548656 69548760 69548656 ID=MS.gene071404.t1.exon1;Parent=MS.gene071404.t1
chr2.4 CDS 69548656 69548760 69548656 ID=cds.MS.gene071404.t1;Parent=MS.gene071404.t1
chr2.4 exon 69547050 69547286 69547050 ID=MS.gene071404.t1.exon2;Parent=MS.gene071404.t1
chr2.4 CDS 69547050 69547286 69547050 ID=cds.MS.gene071404.t1;Parent=MS.gene071404.t1
chr2.4 exon 69546343 69546410 69546343 ID=MS.gene071404.t1.exon3;Parent=MS.gene071404.t1
chr2.4 CDS 69546343 69546410 69546343 ID=cds.MS.gene071404.t1;Parent=MS.gene071404.t1
chr2.4 exon 69545856 69546020 69545856 ID=MS.gene071404.t1.exon4;Parent=MS.gene071404.t1
chr2.4 CDS 69545856 69546020 69545856 ID=cds.MS.gene071404.t1;Parent=MS.gene071404.t1
chr2.4 exon 69545461 69545602 69545461 ID=MS.gene071404.t1.exon5;Parent=MS.gene071404.t1
chr2.4 CDS 69545461 69545602 69545461 ID=cds.MS.gene071404.t1;Parent=MS.gene071404.t1
Gene Sequence

>MS.gene071404

ATGACTAAGAGGCCACAAATATATCTTTTCGGTGATTCGATCACTGAAGAATCCTTTGGTGTTGGAGGATGGGGTGCTTCTCTTGCCAACTATTTCTCTCGTACGGTAATAATTCATTCTTGCTTTAAGTGACCAACCATGTTTCCATGTCATTTCATGTTTTTTCTCTTGATGGTTATTTTTCCAGATTTGTAATAGATTGAAATGAAAGTGATTTTAAATAAAAATAAACAATGTTTTTATTTTTCGTTATGGTTTACAAATCATTCAAGTTGAAAAACTATACATGTTAAATTAGATTTTCATTAATATGATTTAAAGAAAGATAGATTCAGCAATCGATTGATCCCGATAAGATATTTGAGCCTCTTAAATATGTGGTCAAGAGTTCAATTTTTAGTTCACACTATGGAAAATATTTGTTAGGAGAGAGAATCCACTATGGGTGTGTTTGGTAAAAATAAGCTATAAGCTAGCTGATAGCTGATAAGCTAGCTGATAGATGAAAAGCTAGCTTATAGCTGATGACTGATAGCTGATAGCTTATAGCTTAAAAGTTAGCTTATTAAAATTAAAAGTGTTTGGTAAAATTAGCTGTTGAAGTGACTGATAAATATAAATGACGTAAAAGGACGTGTTTGTTTAATTTTTAATATAAATCATATATATAATTTTTATTACTTAACGTATTTCTTTTTAAAGATTTAAATATATTACAAATTATTTTCATCTTTAAAATGATTTTTTTTTTGGAAAACATCTTTAAAAGGATAAATATATATGAATTCAATCGATATTTTCTATTCGACAAAACTTTTTTTTATGAATTTTATTTAACTAAATTTGCTTCACTGTTTATTGTAAATTTTTATAAAATTATTTATTATATGAATTATGAACAAGGTAAAAAAAACAAAAAAAGTGAACAAGAAATCTTTAAAGGAAACCGTAATATATGACAACCTGGGTTATGGGTAAACGTGGACAGTTTACCACAAGAATGTTGCTGTTTTTTTTTATTATAAAAAATAAAATAAAATAAATAAAAGGTTAATTTTGGAAGAAAATATAAAAAGCTACAAGCTAAAAGCTCAAAAGCTACTTGAAATAGCTCTTCAAAAAACGCTATAAGCTAGTGGAAAAAGCTTTTTACCAAACACGTCTTATTCTATCAAAACAAGCTTATAAGCTAGTCCAACAAGCTATAAGCTAGCTTATTTGTGTTACCAAACACAGCCTATATCTGTTTCACATGTTTTCCAACATAGATTAGTCATTGTTAAACAACTTCGTACTAATTTCATGTAACCAAAAATAATTTTTTTTTAATTTTCAAAAACTAATTAAAGTAATTGTTACATTTTCAAACTTTTTTTTTACCAAAATGATTTTTGAGAAAGTACACACAAACATAATTAAAAGATCAAAAGTGATTTTCTCAAAATATTTTTAATCAAATGAGCTCGTGTGATAGGCTGATGTGGTGTTCAGAGGGTATCGTGGGTATAACACACGATGGGCACTGAAGGTGTTGGAAAGGGTTTTTCCAGTATCACATGGCGGTGACGAGGGAACCGAAACTGCTCCTGTTGCTTTGACAGTTTTCTTTGGGGCTAATGATGCTTGTCTTCCTAATAGATGCTCTGTTTTTCAACATGTTCCTCTTCATGAGTACAAGGACAACCTTCGCTCCATTGTCTCCTTTTTTAAGGTAACTTGGTAACAAGTTCTGATTTCTTCAATTATTTATTTTTGTTTATTATTGTTCATTCATAAGAGCGATAAACATAAATGAAGAGTTAAGGAGACCGGCTTTTAAAATAAGATTTTTTTTTTAAAATCAAGAGTTAATGAAACCTAATAACGGGTTTATTTATTAAAAAAATCTATCATGTGCAACTTTGATATTAGAACTTTTTTTTTGAAAGAAATGTGCAAATTTGATATTGGAACTTATTAATTAGTGAGTTGGGATCTGATTCCTCTAAAATGAAAAACTCAAAGTAAATATTAAATCAAACTGTAGAAATAGTAGTTCATGCAAATCAACCATTAAAATGTAAAATGTCACCATTATATGATAAATCAAATGTAGAAATTGTAGGTTCATGCAAATCATAAGTTTGTATTAACAACTCTTAACTAGCTTCTTAATTTTGTTTTTTCTTTAGGTTTAGGCTCTCTTTTGTTCATGAAAAAACAACTTTTTATTTTCTGACTTTACTCTTTAGCCACTTTAAATGTTTAGAATTTTGTTTGATGACGCTTAAATATTTTTTAATTTCAATTATAGGCCTCTTGTATAAATAAATCATATAATTGGTTACTGCAACTTTCTTTTGAATTTCAGAAGCGATGGCCGTCAACTAATGTTGTCCTTATAACTCCTCCTCCCATAGATGAAGAAGCACGACTTAGGTAATGTTTCATTGACTTTTTTCCTTGATTTTGTCATTGTTTTTAGGGTCATGTTAACTTGTGCCCTTAAGGCACATGTTAAGCAACCCAAAAATGGAAACATTTTCTTAAATTTTGTGCATTCAATTATTGAAAAGTAAAAAAGTTAAAGTCAAATGCATTAATTACAATAGAATATTTCTATTTGAATCATTAACTTGTGCCTTGAGGGCACAAGTTAACATTTCCCTTGTTTTTATTAGGTTTTCTTTGTTTGTGACACACACACACGTATAATTCTATAGAAGTTAATCATACTTAGGAATTCTAAAATTTATTGTAGTTATTTGTTTGTGAACAATCCGGAGGGGCTTCCTGAAAGGACAAATGAAGCTGCTGGTGAATATGCTAAAGCATGCATTTCTGTGGCCAATGAGTGTCAAATACCTTTCATTGATCTTTGGACTAAAATGCAACGGTTCCCTGGATGGGAAAAAGTTTACTTGAGGTTTCCAAAAATTTTACCTTCTTTGTTTATCTTCTCTTACAATACTTTGGAATCTTGATATTAGTATTTACCATTTAATTTAAAATACTAATGAACATGATGGCTTTGTTATAGTTTGATTAAATATGCAAAGATAATTGGAAAATCAATTACCATACAAAGTCAATTTTGATGATGCTAATTAATCAAAGTATGTGATGCTATGGTATTATAGTTTTGGTTTTGATATATGATTATGTTAACTTAAATGCAGTGACGGTTTGCATCTCACTAATGGAGGGAATCAATTTGTGTTTGAGGAAGTAATAAAAAAACTAAGAGATGAAGGTGTGAGTCTAGAATCTATGCAGGTTGATCTCCCCCTTTTAGCTGATAATGATCCAAATGATCACTGA
Protein sequence

>MS.gene071404.t1

MTKRPQIYLFGDSITEESFGVGGWGASLANYFSRTADVVFRGYRGYNTRWALKVLERVFPVSHGGDEGTETAPVALTVFFGANDACLPNRCSVFQHVPLHEYKDNLRSIVSFFKKRWPSTNVVLITPPPIDEEARLSYLFVNNPEGLPERTNEAAGEYAKACISVANECQIPFIDLWTKMQRFPGWEKVYLSDGLHLTNGGNQFVFEEVIKKLRDEGVSLESMQVDLPLLADNDPNDH