Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07141.t1 | KEH43285.1 | 97.7 | 311 | 7 | 0 | 1 | 311 | 2019 | 2329 | 2.20E-180 | 641.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07141.t1 | F4IN58 | 72.2 | 313 | 85 | 2 | 1 | 311 | 2147 | 2459 | 1.0e-133 | 477.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07141.t1 | A0A072VYZ6 | 97.7 | 311 | 7 | 0 | 1 | 311 | 2019 | 2329 | 1.6e-180 | 641.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene07141 | 0.801314 | 9.54E-49 | -1.69E-46 |
| MS.gene049321 | MS.gene07141 | -0.812541 | 4.03E-51 | -1.69E-46 |
| MS.gene049343 | MS.gene07141 | 0.807453 | 5.02E-50 | -1.69E-46 |
| MS.gene049983 | MS.gene07141 | 0.825617 | 4.25E-54 | -1.69E-46 |
| MS.gene052519 | MS.gene07141 | 0.823131 | 1.63E-53 | -1.69E-46 |
| MS.gene052685 | MS.gene07141 | 0.805752 | 1.15E-49 | -1.69E-46 |
| MS.gene054479 | MS.gene07141 | 0.804627 | 1.97E-49 | -1.69E-46 |
| MS.gene057026 | MS.gene07141 | 0.819167 | 1.34E-52 | -1.69E-46 |
| MS.gene058009 | MS.gene07141 | 0.813804 | 2.13E-51 | -1.69E-46 |
| MS.gene058783 | MS.gene07141 | 0.801248 | 9.85E-49 | -1.69E-46 |
| MS.gene060435 | MS.gene07141 | 0.804156 | 2.47E-49 | -1.69E-46 |
| MS.gene060935 | MS.gene07141 | 0.831126 | 1.99E-55 | -1.69E-46 |
| MS.gene060937 | MS.gene07141 | 0.821362 | 4.20E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07141.t1 | MTR_1g089760 | 97.749 | 311 | 7 | 0 | 1 | 311 | 2019 | 2329 | 0.0 | 644 |
| MS.gene07141.t1 | MTR_7g113930 | 70.833 | 312 | 86 | 4 | 1 | 311 | 2155 | 2462 | 2.12e-147 | 458 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07141.t1 | AT2G48060 | 72.204 | 313 | 85 | 2 | 1 | 311 | 2058 | 2370 | 2.06e-153 | 475 |
| MS.gene07141.t1 | AT2G48060 | 72.204 | 313 | 85 | 2 | 1 | 311 | 2147 | 2459 | 3.26e-153 | 474 |
| MS.gene07141.t1 | AT2G48060 | 72.204 | 313 | 85 | 2 | 1 | 311 | 2170 | 2482 | 4.03e-153 | 474 |
Find 0 sgRNAs with CRISPR-Local
Find 102 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAATTACAATGAAATTTTT+CGG | - | 10949:11531-11550 | None:intergenic | 10.0% |
| !!! | AAAATTTTCAATAGCAATTT+AGG | - | 10949:11320-11339 | None:intergenic | 15.0% |
| !!! | AATTTTGAATTCAATTTTTC+AGG | + | 10949:11334-11353 | MS.gene07141:intron | 15.0% |
| !! | CAAAATCTACTTAAATTTGA+TGG | - | 10949:11431-11450 | None:intergenic | 20.0% |
| !! | TTTATATTCATTCATTTGCA+TGG | - | 10949:11779-11798 | None:intergenic | 20.0% |
| !!! | TCAAATTTAAGTAGATTTTG+TGG | + | 10949:11431-11450 | MS.gene07141:CDS | 20.0% |
| !!! | TGATGGATTAATATCTTTAA+AGG | - | 10949:11414-11433 | None:intergenic | 20.0% |
| ! | GATGTTCAAAAAGTTCTTAA+CGG | + | 10949:11153-11172 | MS.gene07141:CDS | 25.0% |
| ! | TTGATTTCAGAAATTCTCAA+GGG | - | 10949:11810-11829 | None:intergenic | 25.0% |
| !! | AATTTAAGTAGATTTTGTGG+AGG | + | 10949:11434-11453 | MS.gene07141:CDS | 25.0% |
| !! | ATTGATTTCAGAAATTCTCA+AGG | - | 10949:11811-11830 | None:intergenic | 25.0% |
| AGCAATTTAGGAACAATCAA+AGG | - | 10949:11308-11327 | None:intergenic | 30.0% | |
| ATATGCATTCAAATAGCCAT+TGG | - | 10949:10917-10936 | None:intergenic | 30.0% | |
| ATTGTATATATCCTCGCAAA+TGG | - | 10949:11939-11958 | None:intergenic | 30.0% | |
| GGAAGATATAGAGAATCAAT+AGG | - | 10949:11123-11142 | None:intergenic | 30.0% | |
| GGCAATATTAGAAACACTAT+GGG | - | 10949:11571-11590 | None:intergenic | 30.0% | |
| TATGCATTCAAATAGCCATT+GGG | - | 10949:10916-10935 | None:intergenic | 30.0% | |
| TATGGCAATCATAATATCTG+AGG | + | 10949:11469-11488 | MS.gene07141:CDS | 30.0% | |
| TCAGATATTATGATTGCCAT+AGG | - | 10949:11470-11489 | None:intergenic | 30.0% | |
| TGAAAGCTAGCATCTTTAAT+GGG | - | 10949:10793-10812 | None:intergenic | 30.0% | |
| TGGCAATATTAGAAACACTA+TGG | - | 10949:11572-11591 | None:intergenic | 30.0% | |
| TTGTTACATGCTTCTAATGT+AGG | + | 10949:11906-11925 | MS.gene07141:intron | 30.0% | |
| !! | ACACTATGGGAAGTATTATT+AGG | - | 10949:11558-11577 | None:intergenic | 30.0% |
| !! | TTTTTCGGAGAAAAATTGCA+TGG | - | 10949:11516-11535 | None:intergenic | 30.0% |
| !! | TTTTTCTGGAAAGAAAGCTA+TGG | - | 10949:11754-11773 | None:intergenic | 30.0% |
| !!! | ACATGGAAATTTTTTTCACC+TGG | + | 10949:11046-11065 | MS.gene07141:CDS | 30.0% |
| AAGCTGATGCTAGTACATTA+TGG | + | 10949:10968-10987 | MS.gene07141:CDS | 35.0% | |
| ATATACAATGCAAGAGCTGA+GGG | + | 10949:11950-11969 | MS.gene07141:CDS | 35.0% | |
| ATATTGCCATATTGCTTCAG+AGG | + | 10949:11583-11602 | MS.gene07141:intron | 35.0% | |
| ATTGATATCAAGACAGAGAG+TGG | + | 10949:10814-10833 | MS.gene07141:CDS | 35.0% | |
| CATGTCAGAATACCATTCAA+GGG | - | 10949:11031-11050 | None:intergenic | 35.0% | |
| CTGAAAGCTAGCATCTTTAA+TGG | - | 10949:10794-10813 | None:intergenic | 35.0% | |
| CTTATTTAAGAGACATCCTG+TGG | + | 10949:11867-11886 | MS.gene07141:intron | 35.0% | |
| GAAAGCTAGCATCTTTAATG+GGG | - | 10949:10792-10811 | None:intergenic | 35.0% | |
| TATATACAATGCAAGAGCTG+AGG | + | 10949:11949-11968 | MS.gene07141:CDS | 35.0% | |
| TATTGCCATATTGCTTCAGA+GGG | + | 10949:11584-11603 | MS.gene07141:intron | 35.0% | |
| TTCTTTCACACAGAGTAGTT+TGG | - | 10949:10855-10874 | None:intergenic | 35.0% | |
| TTTCACCTGGATACTTTCAA+GGG | + | 10949:11059-11078 | MS.gene07141:CDS | 35.0% | |
| ! | ATAACCTTTGTTCTAGCTGT+TGG | + | 10949:11653-11672 | MS.gene07141:CDS | 35.0% |
| ! | TTCACAACTTCTTTCCCTTT+TGG | - | 10949:11090-11109 | None:intergenic | 35.0% |
| ! | TTTTCACCTGGATACTTTCA+AGG | + | 10949:11058-11077 | MS.gene07141:CDS | 35.0% |
| ! | TTTTGAACATCAGACTGTGT+TGG | - | 10949:11144-11163 | None:intergenic | 35.0% |
| !!! | TCATTTGCATGGCTTTTTTC+TGG | - | 10949:11768-11787 | None:intergenic | 35.0% |
| AAAGCTATGGTACCTATCAC+AGG | - | 10949:11741-11760 | None:intergenic | 40.0% | |
| ACATGTTGTTCAGACTAGAC+TGG | + | 10949:10999-11018 | MS.gene07141:CDS | 40.0% | |
| ACTCAGCAAGTTCAGCATAT+GGG | + | 10949:11622-11641 | MS.gene07141:CDS | 40.0% | |
| AGTCTAGTCTGAACAACATG+TGG | - | 10949:11000-11019 | None:intergenic | 40.0% | |
| ATATCAAGACAGAGAGTGGA+AGG | + | 10949:10818-10837 | MS.gene07141:CDS | 40.0% | |
| ATGGGGTTTGCAATGTTTGT+TGG | - | 10949:10775-10794 | None:intergenic | 40.0% | |
| CCATGTCAGAATACCATTCA+AGG | - | 10949:11032-11051 | None:intergenic | 40.0% | |
| CCTTGAATGGTATTCTGACA+TGG | + | 10949:11029-11048 | MS.gene07141:CDS | 40.0% | |
| GAAGTAACGTAAGTTACCAC+AGG | - | 10949:11886-11905 | None:intergenic | 40.0% | |
| GGACACTAGTGAAGATCTAT+CGG | + | 10949:12002-12021 | MS.gene07141:CDS | 40.0% | |
| TAAATCGTGAGCAGTTTGAG+TGG | + | 10949:11384-11403 | MS.gene07141:CDS | 40.0% | |
| TCATCTATTACCTCCTCCAA+AGG | - | 10949:11252-11271 | None:intergenic | 40.0% | |
| TCTGTGTGAAAGAATCCAGT+GGG | + | 10949:10861-10880 | MS.gene07141:CDS | 40.0% | |
| TCTTCACTAGTGTCCAGTAA+AGG | - | 10949:11997-12016 | None:intergenic | 40.0% | |
| TGGGGTTTGCAATGTTTGTT+GGG | - | 10949:10774-10793 | None:intergenic | 40.0% | |
| ! | AGATTTTGTGGAGGTTTGAC+AGG | + | 10949:11443-11462 | MS.gene07141:CDS | 40.0% |
| ! | GTGTATTCAAGAAGCATGTG+CGG | - | 10949:12031-12050 | None:intergenic | 40.0% |
| ! | TGTATTCAAGAAGCATGTGC+GGG | - | 10949:12030-12049 | None:intergenic | 40.0% |
| !! | ATTGCTTCAGAGGGTATTCT+TGG | + | 10949:11593-11612 | MS.gene07141:intron | 40.0% |
| !! | GAATACCCTCTGAAGCAATA+TGG | - | 10949:11592-11611 | None:intergenic | 40.0% |
| AATTGCATGGCCAACTTACG+TGG | - | 10949:11503-11522 | None:intergenic | 45.0% | |
| AGAACCTACCTTCCTGTGAT+AGG | + | 10949:11726-11745 | MS.gene07141:CDS | 45.0% | |
| CACTCAGCAAGTTCAGCATA+TGG | + | 10949:11621-11640 | MS.gene07141:CDS | 45.0% | |
| CAGCTTGACAGCATATCAAC+TGG | - | 10949:10954-10973 | None:intergenic | 45.0% | |
| CCAAGATACTTTCGTGTCAC+TGG | + | 10949:11210-11229 | MS.gene07141:CDS | 45.0% | |
| CTATGGTACCTATCACAGGA+AGG | - | 10949:11737-11756 | None:intergenic | 45.0% | |
| CTCAGCAAGTTCAGCATATG+GGG | + | 10949:11623-11642 | MS.gene07141:CDS | 45.0% | |
| CTCTGTGTGAAAGAATCCAG+TGG | + | 10949:10860-10879 | MS.gene07141:CDS | 45.0% | |
| GAGTTGAAGAGGTCCTTTAC+TGG | + | 10949:11981-12000 | MS.gene07141:CDS | 45.0% | |
| GATGACAATGCTGATCCCAA+TGG | + | 10949:10898-10917 | MS.gene07141:CDS | 45.0% | |
| TCAGCAAGTTCAGCATATGG+GGG | + | 10949:11624-11643 | MS.gene07141:CDS | 45.0% | |
| TCATCCTTAAGTCAGAGCAC+TGG | - | 10949:11694-11713 | None:intergenic | 45.0% | |
| TTTCAAGGGACAGGCCAAAA+GGG | + | 10949:11073-11092 | MS.gene07141:CDS | 45.0% | |
| ! | GTATTCAAGAAGCATGTGCG+GGG | - | 10949:12029-12048 | None:intergenic | 45.0% |
| !! | CCAGTGACACGAAAGTATCT+TGG | - | 10949:11213-11232 | None:intergenic | 45.0% |
| !! | TACTTTCGTGTCACTGGTTC+AGG | + | 10949:11216-11235 | MS.gene07141:CDS | 45.0% |
| !! | TAGGTTGATAGCCATTTGCG+AGG | + | 10949:11925-11944 | MS.gene07141:intron | 45.0% |
| ACAGGAAGGTAGGTTCTCGT+AGG | - | 10949:11723-11742 | None:intergenic | 50.0% | |
| ACGGCTCGATGAACAGCTTT+AGG | + | 10949:11172-11191 | MS.gene07141:CDS | 50.0% | |
| ATCGTGAGCAGTTTGAGTGG+TGG | + | 10949:11387-11406 | MS.gene07141:CDS | 50.0% | |
| CAGGAAGGTAGGTTCTCGTA+GGG | - | 10949:11722-11741 | None:intergenic | 50.0% | |
| CCTGGATACTTTCAAGGGAC+AGG | + | 10949:11064-11083 | MS.gene07141:CDS | 50.0% | |
| CTGAATTGAGCGAATCCCAC+TGG | - | 10949:10879-10898 | None:intergenic | 50.0% | |
| CTTTCAAGGGACAGGCCAAA+AGG | + | 10949:11072-11091 | MS.gene07141:CDS | 50.0% | |
| GACTCCAGTGCTCTGACTTA+AGG | + | 10949:11687-11706 | MS.gene07141:CDS | 50.0% | |
| GCGTCCAACAGCTAGAACAA+AGG | - | 10949:11660-11679 | None:intergenic | 50.0% | |
| GGAAACACCACCACGTAAGT+TGG | + | 10949:11490-11509 | MS.gene07141:intron | 50.0% | |
| GGTACCTATCACAGGAAGGT+AGG | - | 10949:11733-11752 | None:intergenic | 50.0% | |
| TCGTGAGCAGTTTGAGTGGT+GGG | + | 10949:11388-11407 | MS.gene07141:CDS | 50.0% | |
| TGGAGGTTTGACAGGACCTA+TGG | + | 10949:11451-11470 | MS.gene07141:CDS | 50.0% | |
| TTCAGGTGACGTTAGGCCTT+TGG | + | 10949:11233-11252 | MS.gene07141:CDS | 50.0% | |
| ! | GACTGGTTCAGTCCCTTGAA+TGG | + | 10949:11016-11035 | MS.gene07141:CDS | 50.0% |
| !! | CCTGTCCCTTGAAAGTATCC+AGG | - | 10949:11067-11086 | None:intergenic | 50.0% |
| !! | TCACTGGTTCAGGTGACGTT+AGG | + | 10949:11226-11245 | MS.gene07141:CDS | 50.0% |
| AGGTGACGTTAGGCCTTTGG+AGG | + | 10949:11236-11255 | MS.gene07141:CDS | 55.0% | |
| GGGTGAGCTTGGAGTTGAAG+AGG | + | 10949:11970-11989 | MS.gene07141:CDS | 55.0% | |
| TGCATGGCCAACTTACGTGG+TGG | - | 10949:11500-11519 | None:intergenic | 55.0% | |
| ! | TGACGTTAGGCCTTTGGAGG+AGG | + | 10949:11239-11258 | MS.gene07141:CDS | 55.0% |
| GCAAGAGCTGAGGGTGAGCT+TGG | + | 10949:11959-11978 | MS.gene07141:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 10949 | gene | 10754 | 12063 | 10754 | ID=MS.gene07141 |
| 10949 | mRNA | 10754 | 12063 | 10754 | ID=MS.gene07141.t1;Parent=MS.gene07141 |
| 10949 | exon | 10754 | 11260 | 10754 | ID=MS.gene07141.t1.exon1;Parent=MS.gene07141.t1 |
| 10949 | CDS | 10754 | 11260 | 10754 | ID=cds.MS.gene07141.t1;Parent=MS.gene07141.t1 |
| 10949 | exon | 11356 | 11503 | 11356 | ID=MS.gene07141.t1.exon2;Parent=MS.gene07141.t1 |
| 10949 | CDS | 11356 | 11503 | 11356 | ID=cds.MS.gene07141.t1;Parent=MS.gene07141.t1 |
| 10949 | exon | 11603 | 11747 | 11603 | ID=MS.gene07141.t1.exon3;Parent=MS.gene07141.t1 |
| 10949 | CDS | 11603 | 11747 | 11603 | ID=cds.MS.gene07141.t1;Parent=MS.gene07141.t1 |
| 10949 | exon | 11928 | 12063 | 11928 | ID=MS.gene07141.t1.exon4;Parent=MS.gene07141.t1 |
| 10949 | CDS | 11928 | 12063 | 11928 | ID=cds.MS.gene07141.t1;Parent=MS.gene07141.t1 |
| Gene Sequence |
| Protein sequence |
