Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071586.t1 | XP_013455822.1 | 100 | 76 | 0 | 0 | 4 | 79 | 363 | 438 | 3.10E-33 | 150.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071586.t1 | Q9ASP6 | 46.2 | 80 | 38 | 3 | 4 | 79 | 369 | 447 | 3.8e-08 | 58.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071586.t1 | A0A072ULD6 | 100.0 | 76 | 0 | 0 | 4 | 79 | 363 | 438 | 2.2e-33 | 150.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene071586 | 0.806942 | 6.44E-50 | -1.69E-46 |
| MS.gene050442 | MS.gene071586 | 0.81295 | 3.27E-51 | -1.69E-46 |
| MS.gene053008 | MS.gene071586 | 0.811923 | 5.49E-51 | -1.69E-46 |
| MS.gene05517 | MS.gene071586 | 0.802591 | 5.22E-49 | -1.69E-46 |
| MS.gene057693 | MS.gene071586 | 0.80751 | 4.88E-50 | -1.69E-46 |
| MS.gene059842 | MS.gene071586 | 0.831238 | 1.87E-55 | -1.69E-46 |
| MS.gene060802 | MS.gene071586 | 0.814443 | 1.54E-51 | -1.69E-46 |
| MS.gene060803 | MS.gene071586 | 0.837387 | 5.36E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071586.t1 | MTR_4g052940 | 100.000 | 76 | 0 | 0 | 4 | 79 | 363 | 438 | 3.95e-47 | 156 |
| MS.gene071586.t1 | MTR_7g010360 | 79.221 | 77 | 16 | 0 | 3 | 79 | 602 | 678 | 3.72e-36 | 128 |
| MS.gene071586.t1 | MTR_7g104800 | 68.831 | 77 | 23 | 1 | 4 | 79 | 351 | 427 | 1.05e-15 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071586.t1 | AT3G52660 | 54.430 | 79 | 32 | 2 | 4 | 79 | 347 | 424 | 1.48e-19 | 81.3 |
| MS.gene071586.t1 | AT3G52660 | 54.430 | 79 | 32 | 2 | 4 | 79 | 347 | 424 | 1.48e-19 | 81.3 |
| MS.gene071586.t1 | AT3G52660 | 54.430 | 79 | 32 | 2 | 4 | 79 | 347 | 424 | 1.48e-19 | 81.3 |
| MS.gene071586.t1 | AT4G00830 | 47.500 | 80 | 37 | 3 | 4 | 79 | 369 | 447 | 1.58e-13 | 63.9 |
| MS.gene071586.t1 | AT4G00830 | 47.500 | 80 | 37 | 3 | 4 | 79 | 369 | 447 | 1.58e-13 | 63.9 |
| MS.gene071586.t1 | AT4G00830 | 47.500 | 80 | 37 | 3 | 4 | 79 | 369 | 447 | 1.58e-13 | 63.9 |
| MS.gene071586.t1 | AT4G00830 | 47.500 | 80 | 37 | 3 | 4 | 79 | 369 | 447 | 1.69e-13 | 63.9 |
| MS.gene071586.t1 | AT4G00830 | 47.500 | 80 | 37 | 3 | 4 | 79 | 361 | 439 | 2.35e-13 | 63.5 |
| MS.gene071586.t1 | AT4G00830 | 47.500 | 80 | 37 | 3 | 4 | 79 | 361 | 439 | 2.35e-13 | 63.5 |
Find 37 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCCATGATGCCGATGCTTC+TGG | 0.302055 | 4.4:-49272123 | MS.gene071586:CDS |
| TATCCGCCACCTGTTGGTTA+TGG | 0.346369 | 4.4:-49272867 | MS.gene071586:CDS |
| TTTACTCAGCCTTTGATGTA+TGG | 0.351303 | 4.4:-49272170 | MS.gene071586:intron |
| GGTGCACTTGGTGCAGGATA+TGG | 0.362461 | 4.4:-49272825 | MS.gene071586:CDS |
| CCAACAGGTGGCGGATAACT+TGG | 0.370029 | 4.4:+49272873 | None:intergenic |
| CCTGTTGGTTATGGTTTGGT+TGG | 0.436777 | 4.4:-49272858 | MS.gene071586:CDS |
| CTGTTGGTTATGGTTTGGTT+GGG | 0.444871 | 4.4:-49272857 | MS.gene071586:CDS |
| ATGCCGATGCTTCTGGCTGA+TGG | 0.461515 | 4.4:-49272116 | MS.gene071586:CDS |
| GGTTTGGTTGGGAATCCATA+TGG | 0.472566 | 4.4:-49272846 | MS.gene071586:CDS |
| GCCACCTGTTGGTTATGGTT+TGG | 0.476814 | 4.4:-49272862 | MS.gene071586:CDS |
| CCTGCACCAAGTGCACCATA+TGG | 0.484549 | 4.4:+49272831 | None:intergenic |
| GGTGCAGGATATGGTGCTCC+TGG | 0.485631 | 4.4:-49272816 | MS.gene071586:CDS |
| CCATATGGTGCACTTGGTGC+AGG | 0.490417 | 4.4:-49272831 | MS.gene071586:CDS |
| TCAGCCTTTGATGTATGGAC+CGG | 0.491896 | 4.4:-49272165 | MS.gene071586:intron |
| TGGTGCTCCTGGTCTTGCTC+AGG | 0.493057 | 4.4:-49272805 | MS.gene071586:intron |
| TATGGACCGGGTCAAACTCC+TGG | 0.500903 | 4.4:-49272152 | MS.gene071586:CDS |
| AAGCCACCAGGCTGATCAGA+AGG | 0.524101 | 4.4:-49272931 | MS.gene071586:CDS |
| CCAAGTTATCCGCCACCTGT+TGG | 0.524145 | 4.4:-49272873 | MS.gene071586:CDS |
| CGTCTGTGTATTTGATACTA+CGG | 0.526755 | 4.4:+49272908 | None:intergenic |
| GGCATCATGGCCATTCCACC+AGG | 0.528246 | 4.4:+49272134 | None:intergenic |
| CCAACCAAACCATAACCAAC+AGG | 0.529349 | 4.4:+49272858 | None:intergenic |
| GGGTCAAACTCCTGGTGGAA+TGG | 0.533060 | 4.4:-49272144 | MS.gene071586:CDS |
| AGCCAGAAGCATCGGCATCA+TGG | 0.537312 | 4.4:+49272121 | None:intergenic |
| CGTCCATCAGCCAGAAGCAT+CGG | 0.547362 | 4.4:+49272113 | None:intergenic |
| GGGAATCCATATGGTGCACT+TGG | 0.560561 | 4.4:-49272837 | MS.gene071586:CDS |
| AAACCATAACCAACAGGTGG+CGG | 0.573239 | 4.4:+49272864 | None:intergenic |
| AAGATCACCTGAGCAAGACC+AGG | 0.576660 | 4.4:+49272798 | None:intergenic |
| CAGCCTTTGATGTATGGACC+GGG | 0.587599 | 4.4:-49272164 | MS.gene071586:CDS |
| TGTTCTTTAGCAAAGCCACC+AGG | 0.593549 | 4.4:-49272943 | None:intergenic |
| CTACGGCCTTCTGATCAGCC+TGG | 0.596585 | 4.4:+49272925 | None:intergenic |
| CTTCTGGCTGATGGACGCAT+CGG | 0.601935 | 4.4:-49272107 | MS.gene071586:CDS |
| ATTCCACCAGGAGTTTGACC+CGG | 0.610475 | 4.4:+49272146 | None:intergenic |
| TGACCCGGTCCATACATCAA+AGG | 0.637581 | 4.4:+49272161 | None:intergenic |
| GTATCAAATACACAGACGCA+AGG | 0.658627 | 4.4:-49272903 | MS.gene071586:CDS |
| GGACCGGGTCAAACTCCTGG+TGG | 0.664975 | 4.4:-49272149 | MS.gene071586:CDS |
| CGGCCTTCTGATCAGCCTGG+TGG | 0.667659 | 4.4:+49272928 | None:intergenic |
| ACCAAACCATAACCAACAGG+TGG | 0.672729 | 4.4:+49272861 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATTCATTAACTAGTTTAA+AGG | + | chr4.4:49272480-49272499 | None:intergenic | 15.0% |
| !! | AACTTCTATGTAAAAATACT+TGG | + | chr4.4:49272759-49272778 | None:intergenic | 20.0% |
| !! | ACTTCTATGTAAAAATACTT+GGG | + | chr4.4:49272758-49272777 | None:intergenic | 20.0% |
| !! | ATTTCTCTTATAAAAAGAAC+CGG | + | chr4.4:49272429-49272448 | None:intergenic | 20.0% |
| !! | TAAACTTACAAGTAAAATAG+AGG | - | chr4.4:49272582-49272601 | MS.gene071586:intron | 20.0% |
| ! | GAGTGTTAAATATGTAAAAG+AGG | + | chr4.4:49272403-49272422 | None:intergenic | 25.0% |
| !! | TAAGAGACATTGAGTATTTT+TGG | + | chr4.4:49272653-49272672 | None:intergenic | 25.0% |
| !!! | AAGTAAAATAGAGGTATTGA+AGG | - | chr4.4:49272591-49272610 | MS.gene071586:intron | 25.0% |
| AGATTAATTTATGCTGAACG+TGG | + | chr4.4:49272813-49272832 | None:intergenic | 30.0% | |
| ATTAATTTATGCTGAACGTG+GGG | + | chr4.4:49272811-49272830 | None:intergenic | 30.0% | |
| CTAGTTTAAAGGAATTTCTG+TGG | + | chr4.4:49272469-49272488 | None:intergenic | 30.0% | |
| CTCTTATAAACAAGATCAGA+GGG | - | chr4.4:49272667-49272686 | MS.gene071586:intron | 30.0% | |
| GATTAATTTATGCTGAACGT+GGG | + | chr4.4:49272812-49272831 | None:intergenic | 30.0% | |
| GTAAGTGGAGACATTAATAA+TGG | + | chr4.4:49272534-49272553 | None:intergenic | 30.0% | |
| TCTCTTATAAACAAGATCAG+AGG | - | chr4.4:49272666-49272685 | MS.gene071586:intron | 30.0% | |
| ! | AATGTCACTATTTGCTAGAA+TGG | + | chr4.4:49272360-49272379 | None:intergenic | 30.0% |
| !! | GAGACATTAATAATGGACAA+CGG | + | chr4.4:49272527-49272546 | None:intergenic | 30.0% |
| !! | TGTTTTCATTGTGTTACTGT+TGG | - | chr4.4:49272324-49272343 | MS.gene071586:intron | 30.0% |
| !!! | TAATGGACAACGGTATTTTT+TGG | + | chr4.4:49272517-49272536 | None:intergenic | 30.0% |
| ATTAATGTCTCCACTTACCT+GGG | - | chr4.4:49272536-49272555 | MS.gene071586:intron | 35.0% | |
| CGTCTGTGTATTTGATACTA+CGG | + | chr4.4:49272125-49272144 | None:intergenic | 35.0% | |
| TATTAATGTCTCCACTTACC+TGG | - | chr4.4:49272535-49272554 | MS.gene071586:intron | 35.0% | |
| TCTCTTATAAAAAGAACCGG+CGG | + | chr4.4:49272426-49272445 | None:intergenic | 35.0% | |
| TTTACTCAGCCTTTGATGTA+TGG | - | chr4.4:49272860-49272879 | MS.gene071586:CDS | 35.0% | |
| ! | AAAGAGGTATGCATGCAATA+AGG | + | chr4.4:49272387-49272406 | None:intergenic | 35.0% |
| ! | TAATCTGAGATTCTGATGAC+TGG | - | chr4.4:49272827-49272846 | MS.gene071586:CDS | 35.0% |
| CATGATACTGACAAGTCTGA+TGG | + | chr4.4:49272733-49272752 | None:intergenic | 40.0% | |
| CTCTTATAAAAAGAACCGGC+GGG | + | chr4.4:49272425-49272444 | None:intergenic | 40.0% | |
| GTATCAAATACACAGACGCA+AGG | - | chr4.4:49272127-49272146 | MS.gene071586:CDS | 40.0% | |
| TACATATTTAACACTCCCGC+CGG | - | chr4.4:49272407-49272426 | MS.gene071586:intron | 40.0% | |
| TGAACGTGGGGATAAATCAA+TGG | + | chr4.4:49272799-49272818 | None:intergenic | 40.0% | |
| TTAATGTCTCCACTTACCTG+GGG | - | chr4.4:49272537-49272556 | MS.gene071586:intron | 40.0% | |
| ! | ACAAAATTCCCATTTTCCCC+AGG | + | chr4.4:49272556-49272575 | None:intergenic | 40.0% |
| !! | CTGTTGGTTATGGTTTGGTT+GGG | - | chr4.4:49272173-49272192 | MS.gene071586:intron | 40.0% |
| AAACCATAACCAACAGGTGG+CGG | + | chr4.4:49272169-49272188 | None:intergenic | 45.0% | |
| ACCAAACCATAACCAACAGG+TGG | + | chr4.4:49272172-49272191 | None:intergenic | 45.0% | |
| CCAACCAAACCATAACCAAC+AGG | + | chr4.4:49272175-49272194 | None:intergenic | 45.0% | |
| TCAGCCTTTGATGTATGGAC+CGG | - | chr4.4:49272865-49272884 | MS.gene071586:CDS | 45.0% | |
| TCCACTTACCTGGGGAAAAT+GGG | - | chr4.4:49272545-49272564 | MS.gene071586:intron | 45.0% | |
| ! | GGTTTGGTTGGGAATCCATA+TGG | - | chr4.4:49272184-49272203 | MS.gene071586:intron | 45.0% |
| !! | CCTGTTGGTTATGGTTTGGT+TGG | - | chr4.4:49272172-49272191 | MS.gene071586:intron | 45.0% |
| AAGATCACCTGAGCAAGACC+AGG | + | chr4.4:49272235-49272254 | None:intergenic | 50.0% | |
| ATTCCACCAGGAGTTTGACC+CGG | + | chr4.4:49272887-49272906 | None:intergenic | 50.0% | |
| CAGCCTTTGATGTATGGACC+GGG | - | chr4.4:49272866-49272885 | MS.gene071586:CDS | 50.0% | |
| CTCCACTTACCTGGGGAAAA+TGG | - | chr4.4:49272544-49272563 | MS.gene071586:intron | 50.0% | |
| GGGAATCCATATGGTGCACT+TGG | - | chr4.4:49272193-49272212 | MS.gene071586:intron | 50.0% | |
| TGACCCGGTCCATACATCAA+AGG | + | chr4.4:49272872-49272891 | None:intergenic | 50.0% | |
| ! | GCCACCTGTTGGTTATGGTT+TGG | - | chr4.4:49272168-49272187 | MS.gene071586:intron | 50.0% |
| ! | TATCCGCCACCTGTTGGTTA+TGG | - | chr4.4:49272163-49272182 | MS.gene071586:CDS | 50.0% |
| ! | TCCCATTTTCCCCAGGTAAG+TGG | + | chr4.4:49272549-49272568 | None:intergenic | 50.0% |
| AAGCCACCAGGCTGATCAGA+AGG | - | chr4.4:49272099-49272118 | MS.gene071586:CDS | 55.0% | |
| CCAAGTTATCCGCCACCTGT+TGG | - | chr4.4:49272157-49272176 | MS.gene071586:CDS | 55.0% | |
| CCTGCACCAAGTGCACCATA+TGG | + | chr4.4:49272202-49272221 | None:intergenic | 55.0% | |
| GGGTCAAACTCCTGGTGGAA+TGG | - | chr4.4:49272886-49272905 | MS.gene071586:CDS | 55.0% | |
| TATGGACCGGGTCAAACTCC+TGG | - | chr4.4:49272878-49272897 | MS.gene071586:CDS | 55.0% | |
| ! | ATGCCGATGCTTCTGGCTGA+TGG | - | chr4.4:49272914-49272933 | MS.gene071586:CDS | 55.0% |
| ! | CGTCCATCAGCCAGAAGCAT+CGG | + | chr4.4:49272920-49272939 | None:intergenic | 55.0% |
| ! | CTTCTGGCTGATGGACGCAT+CGG | - | chr4.4:49272923-49272942 | MS.gene071586:CDS | 55.0% |
| !! | AGCCAGAAGCATCGGCATCA+TGG | + | chr4.4:49272912-49272931 | None:intergenic | 55.0% |
| !! | CCAACAGGTGGCGGATAACT+TGG | + | chr4.4:49272160-49272179 | None:intergenic | 55.0% |
| !! | CCATATGGTGCACTTGGTGC+AGG | - | chr4.4:49272199-49272218 | MS.gene071586:intron | 55.0% |
| !! | GGTGCACTTGGTGCAGGATA+TGG | - | chr4.4:49272205-49272224 | MS.gene071586:intron | 55.0% |
| CTACGGCCTTCTGATCAGCC+TGG | + | chr4.4:49272108-49272127 | None:intergenic | 60.0% | |
| GGCATCATGGCCATTCCACC+AGG | + | chr4.4:49272899-49272918 | None:intergenic | 60.0% | |
| GGCCATGATGCCGATGCTTC+TGG | - | chr4.4:49272907-49272926 | MS.gene071586:CDS | 60.0% | |
| GGTGCAGGATATGGTGCTCC+TGG | - | chr4.4:49272214-49272233 | MS.gene071586:intron | 60.0% | |
| !! | TGGTGCTCCTGGTCTTGCTC+AGG | - | chr4.4:49272225-49272244 | MS.gene071586:intron | 60.0% |
| CGGCCTTCTGATCAGCCTGG+TGG | + | chr4.4:49272105-49272124 | None:intergenic | 65.0% | |
| GGACCGGGTCAAACTCCTGG+TGG | - | chr4.4:49272881-49272900 | MS.gene071586:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 49272097 | 49272955 | 49272097 | ID=MS.gene071586 |
| chr4.4 | mRNA | 49272097 | 49272955 | 49272097 | ID=MS.gene071586.t1;Parent=MS.gene071586 |
| chr4.4 | exon | 49272806 | 49272955 | 49272806 | ID=MS.gene071586.t1.exon1;Parent=MS.gene071586.t1 |
| chr4.4 | CDS | 49272806 | 49272955 | 49272806 | ID=cds.MS.gene071586.t1;Parent=MS.gene071586.t1 |
| chr4.4 | exon | 49272097 | 49272183 | 49272097 | ID=MS.gene071586.t1.exon2;Parent=MS.gene071586.t1 |
| chr4.4 | CDS | 49272097 | 49272183 | 49272097 | ID=cds.MS.gene071586.t1;Parent=MS.gene071586.t1 |
| Gene Sequence |
| Protein sequence |
