AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene05517


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05517.t1 MTR_6g486270 100.000 56 0 0 1 56 315 370 6.27e-31 110
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 8 sgRNAs with CRISPR-Local

Find 12 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGCAGAAGAAGCAGATTT+TGG 0.210644 6.2:+84512461 MS.gene05517:CDS
GGAGTTGGAGAAGGTGAGGT+TGG 0.470646 6.2:+84512419 MS.gene05517:CDS
GGCTGAAATATCGAAAATAC+AGG 0.472979 6.2:+84512494 MS.gene05517:CDS
GAGGCAACAGATGATGGAGT+TGG 0.516973 6.2:+84512404 None:intergenic
ACAGATGATGGAGTTGGAGA+AGG 0.562220 6.2:+84512410 None:intergenic
TGATGGAGTTGGAGAAGGTG+AGG 0.617953 6.2:+84512415 MS.gene05517:CDS
TGATGCTGATGGTGACGATG+AGG 0.699210 6.2:+84512527 MS.gene05517:CDS
GAAGCAGTAGATGATGCTGA+TGG 0.735151 6.2:+84512516 MS.gene05517:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTTGGATTTTAATAGGGAAT+TGG + chr6.2:84512437-84512456 MS.gene05517:CDS 30.0%
GGCTGAAATATCGAAAATAC+AGG + chr6.2:84512494-84512513 MS.gene05517:CDS 35.0%
! AGAAGAAGCAGATTTTGGAT+AGG + chr6.2:84512466-84512485 MS.gene05517:CDS 35.0%
! GAAGAAGCAGATTTTGGATA+GGG + chr6.2:84512467-84512486 MS.gene05517:CDS 35.0%
!! AGGTGAGGTTGGATTTTAAT+AGG + chr6.2:84512430-84512449 MS.gene05517:CDS 35.0%
!! GGTGAGGTTGGATTTTAATA+GGG + chr6.2:84512431-84512450 MS.gene05517:CDS 35.0%
! GTTGCAGAAGAAGCAGATTT+TGG + chr6.2:84512461-84512480 MS.gene05517:CDS 40.0%
GAAGCAGTAGATGATGCTGA+TGG + chr6.2:84512516-84512535 MS.gene05517:CDS 45.0%
TGATGGAGTTGGAGAAGGTG+AGG + chr6.2:84512415-84512434 MS.gene05517:CDS 50.0%
! GCAGATTTTGGATAGGGCTC+AGG + chr6.2:84512473-84512492 MS.gene05517:CDS 50.0%
! TGATGCTGATGGTGACGATG+AGG + chr6.2:84512527-84512546 MS.gene05517:CDS 50.0%
GGAGTTGGAGAAGGTGAGGT+TGG + chr6.2:84512419-84512438 MS.gene05517:CDS 55.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 84512414 84512584 84512414 ID=MS.gene05517
chr6.2 mRNA 84512414 84512584 84512414 ID=MS.gene05517.t1;Parent=MS.gene05517
chr6.2 exon 84512414 84512584 84512414 ID=MS.gene05517.t1.exon1;Parent=MS.gene05517.t1
chr6.2 CDS 84512414 84512584 84512414 ID=cds.MS.gene05517.t1;Parent=MS.gene05517.t1
Gene Sequence

>MS.gene05517

ATGATGGAGTTGGAGAAGGTGAGGTTGGATTTTAATAGGGAATTGGAGTTGCAGAAGAAGCAGATTTTGGATAGGGCTCAGGCTGAAATATCGAAAATACAGGAAGCAGTAGATGATGCTGATGGTGACGATGAGGACACAGACACTTCTGCTGATAATCTCAGTGAATAA

Protein sequence

>MS.gene05517.t1

MMELEKVRLDFNRELELQKKQILDRAQAEISKIQEAVDDADGDDEDTDTSADNLSE