Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071680.t1 | XP_013453268.2 | 97.3 | 401 | 9 | 1 | 1 | 399 | 1 | 401 | 2.20E-212 | 748 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071680.t1 | Q8GYX8 | 73.9 | 402 | 98 | 5 | 1 | 399 | 1 | 398 | 4.7e-155 | 548.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071680.t1 | A0A396HMW6 | 97.3 | 401 | 9 | 1 | 1 | 399 | 1 | 401 | 1.6e-212 | 748.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056854 | MS.gene071680 | 0.8144 | 1.57E-51 | -1.69E-46 |
| MS.gene05686 | MS.gene071680 | 0.81607 | 6.68E-52 | -1.69E-46 |
| MS.gene060962 | MS.gene071680 | 0.803078 | 4.14E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene071680 | MS.gene028573 | PPI |
| MS.gene006610 | MS.gene071680 | PPI |
| MS.gene071680 | MS.gene026672 | PPI |
| MS.gene071680 | MS.gene045585 | PPI |
| MS.gene071680 | MS.gene33648 | PPI |
| MS.gene071680 | MS.gene065944 | PPI |
| MS.gene071680 | MS.gene33649 | PPI |
| MS.gene065945 | MS.gene071680 | PPI |
| MS.gene071680 | MS.gene31857 | PPI |
| MS.gene071680 | MS.gene40160 | PPI |
| MS.gene071680 | MS.gene031697 | PPI |
| MS.gene071680 | MS.gene93157 | PPI |
| MS.gene071680 | MS.gene31858 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071680.t1 | MTR_5g004570 | 96.930 | 228 | 5 | 1 | 1 | 226 | 1 | 228 | 2.27e-163 | 458 |
| MS.gene071680.t1 | MTR_1g029470 | 57.812 | 320 | 126 | 4 | 1 | 318 | 1 | 313 | 9.63e-132 | 383 |
| MS.gene071680.t1 | MTR_8g037810 | 57.500 | 320 | 130 | 3 | 1 | 316 | 1 | 318 | 1.04e-126 | 369 |
| MS.gene071680.t1 | MTR_5g004600 | 97.714 | 175 | 4 | 0 | 225 | 399 | 4 | 178 | 6.59e-125 | 358 |
| MS.gene071680.t1 | MTR_1g029460 | 52.548 | 314 | 140 | 4 | 1 | 311 | 1 | 308 | 2.83e-111 | 332 |
| MS.gene071680.t1 | MTR_3g027580 | 47.143 | 70 | 35 | 1 | 6 | 73 | 89 | 158 | 6.17e-12 | 67.4 |
| MS.gene071680.t1 | MTR_4g023470 | 52.308 | 65 | 29 | 1 | 7 | 69 | 87 | 151 | 4.29e-11 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071680.t1 | AT1G21080 | 73.671 | 395 | 95 | 6 | 1 | 392 | 1 | 389 | 0.0 | 579 |
| MS.gene071680.t1 | AT1G21080 | 72.277 | 404 | 94 | 6 | 1 | 392 | 1 | 398 | 0.0 | 578 |
| MS.gene071680.t1 | AT1G76700 | 75.260 | 384 | 88 | 5 | 1 | 381 | 1 | 380 | 0.0 | 563 |
| MS.gene071680.t1 | AT1G21080 | 71.336 | 307 | 82 | 4 | 87 | 392 | 1 | 302 | 3.65e-154 | 437 |
| MS.gene071680.t1 | AT1G77020 | 59.259 | 324 | 127 | 3 | 1 | 321 | 1 | 322 | 1.65e-135 | 393 |
| MS.gene071680.t1 | AT4G39150 | 55.385 | 325 | 139 | 3 | 1 | 321 | 1 | 323 | 3.06e-126 | 368 |
| MS.gene071680.t1 | AT4G39150 | 55.385 | 325 | 139 | 3 | 1 | 321 | 1 | 323 | 3.06e-126 | 368 |
| MS.gene071680.t1 | AT4G39150 | 55.385 | 325 | 139 | 3 | 1 | 321 | 1 | 323 | 3.06e-126 | 368 |
| MS.gene071680.t1 | AT2G21510 | 53.774 | 318 | 143 | 2 | 1 | 314 | 1 | 318 | 6.65e-122 | 357 |
| MS.gene071680.t1 | AT2G21510 | 53.774 | 318 | 143 | 2 | 1 | 314 | 1 | 318 | 6.65e-122 | 357 |
| MS.gene071680.t1 | AT2G21510 | 53.774 | 318 | 143 | 2 | 1 | 314 | 1 | 318 | 6.97e-122 | 357 |
| MS.gene071680.t1 | AT2G21510 | 53.774 | 318 | 143 | 2 | 1 | 314 | 1 | 318 | 6.97e-122 | 357 |
| MS.gene071680.t1 | AT2G21510 | 53.774 | 318 | 143 | 2 | 1 | 314 | 1 | 318 | 9.35e-122 | 356 |
| MS.gene071680.t1 | AT1G59980 | 38.710 | 93 | 46 | 3 | 8 | 89 | 25 | 117 | 3.19e-11 | 64.7 |
| MS.gene071680.t1 | AT1G59980 | 38.710 | 93 | 46 | 3 | 8 | 89 | 25 | 117 | 3.24e-11 | 65.1 |
| MS.gene071680.t1 | AT1G59725 | 43.836 | 73 | 37 | 2 | 6 | 74 | 4 | 76 | 7.10e-11 | 63.5 |
Find 102 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCACGTAGTTCTTCTTTCT+TGG | 0.176561 | 5.3:-119847 | None:intergenic |
| GGAGCAGGTCTGTGGGCACC+TGG | 0.248868 | 5.3:-120362 | None:intergenic |
| TACACCAGTACTTGAAGATT+TGG | 0.283792 | 5.3:-120094 | None:intergenic |
| CTTTGTTTCCCTGGACATAC+TGG | 0.285874 | 5.3:-112937 | None:intergenic |
| TATGATGCACATGGCAAATC+TGG | 0.306958 | 5.3:+111770 | MS.gene071680:CDS |
| CACCTGTTGCCGCTGTAACT+TGG | 0.313148 | 5.3:-113469 | None:intergenic |
| AGCAGGTCTGTGGGCACCTG+GGG | 0.316213 | 5.3:-120360 | None:intergenic |
| AATTTATGTACTGCTTTGTT+TGG | 0.332629 | 5.3:-120024 | None:intergenic |
| GGGCAGCAAGAGGGTCATTT+GGG | 0.335034 | 5.3:-111573 | None:intergenic |
| CTAAACCACTCAGCAATAAA+AGG | 0.342495 | 5.3:-113422 | None:intergenic |
| AGGTCTGTGGGCACCTGGGG+GGG | 0.345193 | 5.3:-120357 | None:intergenic |
| TGGGCAGCAAGAGGGTCATT+TGG | 0.353660 | 5.3:-111574 | None:intergenic |
| GTGGAGGCCCCACACTTTGC+AGG | 0.354651 | 5.3:+120299 | MS.gene071680:CDS |
| CCATTGATGCCATGGCTAAC+TGG | 0.362537 | 5.3:-111961 | None:intergenic |
| AGGCTTGTAGAGATACTAAA+AGG | 0.364783 | 5.3:+112905 | MS.gene071680:CDS |
| AACTCTTGGTAAAATCTTCC+AGG | 0.368732 | 5.3:+119887 | MS.gene071680:CDS |
| GCAGGTCTGTGGGCACCTGG+GGG | 0.371126 | 5.3:-120359 | None:intergenic |
| CAGGTCTGTGGGCACCTGGG+GGG | 0.375934 | 5.3:-120358 | None:intergenic |
| ACCTGTTGCCGCTGTAACTT+GGG | 0.383531 | 5.3:-113468 | None:intergenic |
| GTTGTCATTATACAGCTTAT+GGG | 0.384762 | 5.3:+113315 | MS.gene071680:intron |
| GAGCAGGTCTGTGGGCACCT+GGG | 0.389447 | 5.3:-120361 | None:intergenic |
| CATAAGCTTGCCTTTGAGTT+GGG | 0.400101 | 5.3:-111751 | None:intergenic |
| GAACTACGTGCACGAGCAAA+AGG | 0.400470 | 5.3:+119858 | MS.gene071680:CDS |
| AAGTGTGGGGCCTCCACGTA+GGG | 0.400490 | 5.3:-120293 | None:intergenic |
| CAAAGTCTTCTTTGTTTCCC+TGG | 0.407211 | 5.3:-112946 | None:intergenic |
| TCATAAGCTTGCCTTTGAGT+TGG | 0.415070 | 5.3:-111752 | None:intergenic |
| TCTTTCCCGTGTCTGTCAAA+TGG | 0.421026 | 5.3:+119711 | MS.gene071680:CDS |
| GAAGATTTGGGTGAGGTACT+AGG | 0.424180 | 5.3:-120081 | None:intergenic |
| AAGCTTCTTAATCTCAGATT+CGG | 0.424958 | 5.3:-111390 | None:intergenic |
| ACACCAGTACTTGAAGATTT+GGG | 0.429890 | 5.3:-120093 | None:intergenic |
| GTATTCTTCAAGCTCTTCTT+CGG | 0.430532 | 5.3:-119620 | None:intergenic |
| TCGTACCTGCATTGGACAGC+CGG | 0.441407 | 5.3:-112994 | None:intergenic |
| TCCCAAGTTACAGCGGCAAC+AGG | 0.444241 | 5.3:+113467 | MS.gene071680:CDS |
| TCTTCATATCCTCCTGCAGT+TGG | 0.446808 | 5.3:-119570 | None:intergenic |
| TGCTGGTTGGAGCAGGTCTG+TGG | 0.446816 | 5.3:-120370 | None:intergenic |
| CCAGTTAGCCATGGCATCAA+TGG | 0.450422 | 5.3:+111961 | MS.gene071680:CDS |
| ACACCATCATCAATCCTTGC+TGG | 0.451831 | 5.3:-120387 | None:intergenic |
| CAATCCTTGCTGGTTGGAGC+AGG | 0.456091 | 5.3:-120377 | None:intergenic |
| ACAGGCAAGGCAAGTGCATC+CGG | 0.467108 | 5.3:+111543 | MS.gene071680:intron |
| CATGGCATCAATGGCTTCCA+TGG | 0.469034 | 5.3:+111970 | MS.gene071680:CDS |
| AACCCATGCTGTCGCATCGC+AGG | 0.483341 | 5.3:+120119 | MS.gene071680:CDS |
| GATTGCAGCAGGATCAATAA+TGG | 0.492454 | 5.3:-111894 | None:intergenic |
| ATCTCAGATTCGGATGCGGT+TGG | 0.495393 | 5.3:-111380 | None:intergenic |
| GAGTTGGGTCACTGAGAACT+TGG | 0.499446 | 5.3:-111736 | None:intergenic |
| AAAGCAGTACATAAATTGAA+TGG | 0.499865 | 5.3:+120030 | MS.gene071680:CDS |
| GCAAAAGGGTTGAAAACTCT+TGG | 0.501287 | 5.3:+119873 | MS.gene071680:CDS |
| GCTGGTTGGAGCAGGTCTGT+GGG | 0.501375 | 5.3:-120369 | None:intergenic |
| AGGCAGGCAGCAAAAGAGCT+AGG | 0.503290 | 5.3:+113374 | MS.gene071680:CDS |
| TTAGGGCATCGTACCTGCAT+TGG | 0.506072 | 5.3:-113002 | None:intergenic |
| CTTCCGCCAGGTTGTTCAGA+AGG | 0.520281 | 5.3:+112871 | MS.gene071680:intron |
| TTAAGAAGCTTATTACATCA+AGG | 0.529523 | 5.3:+111402 | MS.gene071680:CDS |
| TCACCCAAATCTTCAAGTAC+TGG | 0.532073 | 5.3:+120090 | MS.gene071680:CDS |
| CTGAGTGGTTTAGAAACAAA+GGG | 0.533228 | 5.3:+113432 | MS.gene071680:CDS |
| CAGACTCAACCAGTATGTCC+AGG | 0.533241 | 5.3:+112928 | MS.gene071680:CDS |
| AAGAAACAACTCAGTTCAGA+AGG | 0.534697 | 5.3:+119589 | MS.gene071680:CDS |
| CTTAATCTCAGATTCGGATG+CGG | 0.535257 | 5.3:-111384 | None:intergenic |
| TTCAGAAGGAGAGAGAAGAA+AGG | 0.535912 | 5.3:+112885 | MS.gene071680:CDS |
| GTTGATATGTTGAATACCAT+TGG | 0.536608 | 5.3:+113338 | MS.gene071680:CDS |
| AGCATGGCAAAGATTGCAGC+AGG | 0.536765 | 5.3:-111905 | None:intergenic |
| ATGCAGAAGCTGAAGTAGCC+CGG | 0.542752 | 5.3:+112975 | MS.gene071680:CDS |
| TAGACGGTTAAGACAGCCAA+TGG | 0.543269 | 5.3:+119982 | MS.gene071680:intron |
| AATTGCATTGATCCAACTGC+AGG | 0.548793 | 5.3:+119558 | MS.gene071680:CDS |
| AAAGTGTGGGGCCTCCACGT+AGG | 0.549144 | 5.3:-120294 | None:intergenic |
| CATCATCAATCCTTGCTGGT+TGG | 0.551719 | 5.3:-120383 | None:intergenic |
| CGTACCTGCATTGGACAGCC+GGG | 0.553498 | 5.3:-112993 | None:intergenic |
| CTCTCTGTGGAAGCTTAATG+TGG | 0.565211 | 5.3:+119672 | MS.gene071680:CDS |
| AGCGAATACTATGACGTGCT+TGG | 0.565955 | 5.3:+111350 | MS.gene071680:CDS |
| GTAGCCCGGCTGTCCAATGC+AGG | 0.566818 | 5.3:+112989 | MS.gene071680:CDS |
| GCTTAATGTGGCTGACATTG+AGG | 0.568707 | 5.3:+119684 | MS.gene071680:CDS |
| TTGTCATTATACAGCTTATG+GGG | 0.568921 | 5.3:+113316 | MS.gene071680:intron |
| GCACCTGCGATGCGACAGCA+TGG | 0.568969 | 5.3:-120122 | None:intergenic |
| TGGCTACATATACGCAAGGC+AGG | 0.571362 | 5.3:+113358 | MS.gene071680:CDS |
| AACAACCATTTGACAGACAC+GGG | 0.573149 | 5.3:-119716 | None:intergenic |
| AATTGCATGCCTGCAAAGTG+TGG | 0.575193 | 5.3:-120308 | None:intergenic |
| GCTGAGTGGTTTAGAAACAA+AGG | 0.575329 | 5.3:+113431 | MS.gene071680:CDS |
| TTCTCAGTGACCCAACTCAA+AGG | 0.583766 | 5.3:+111741 | MS.gene071680:CDS |
| GGAAAGATCAATCATGGTGA+AGG | 0.584644 | 5.3:+111325 | None:intergenic |
| GGCAGGCAGCAAAAGAGCTA+GGG | 0.591181 | 5.3:+113375 | MS.gene071680:CDS |
| AAAATTGCAAGACAAAATGC+GGG | 0.593137 | 5.3:+112027 | MS.gene071680:CDS |
| TTGACAGACACGGGAAAGAG+TGG | 0.595512 | 5.3:-119707 | None:intergenic |
| TGCATTGATCCAACTGCAGG+AGG | 0.600589 | 5.3:+119561 | MS.gene071680:CDS |
| AATTTGACTACAACTTCCCT+AGG | 0.603259 | 5.3:+120327 | MS.gene071680:CDS |
| AAACAACCATTTGACAGACA+CGG | 0.605275 | 5.3:-119717 | None:intergenic |
| ATATATAGGCCAGTTAGCCA+TGG | 0.607750 | 5.3:+111952 | MS.gene071680:CDS |
| CATGGAAGCCATTGATGCCA+TGG | 0.610809 | 5.3:-111969 | None:intergenic |
| AATTAATGATTGACTCTCTG+TGG | 0.612175 | 5.3:+119659 | MS.gene071680:CDS |
| AACTACGTGCACGAGCAAAA+GGG | 0.613702 | 5.3:+119859 | MS.gene071680:CDS |
| AGACGGTTAAGACAGCCAAT+GGG | 0.619753 | 5.3:+119983 | MS.gene071680:intron |
| AGGCAAGCTTATGATGCACA+TGG | 0.629597 | 5.3:+111761 | MS.gene071680:CDS |
| ATTGCATGCCTGCAAAGTGT+GGG | 0.632304 | 5.3:-120307 | None:intergenic |
| CACCTGCGATGCGACAGCAT+GGG | 0.633397 | 5.3:-120121 | None:intergenic |
| AGTACTTGAAGATTTGGGTG+AGG | 0.635864 | 5.3:-120088 | None:intergenic |
| AGACTCAACCAGTATGTCCA+GGG | 0.639373 | 5.3:+112929 | MS.gene071680:CDS |
| CCTTGCGTATATGTAGCCAA+TGG | 0.645015 | 5.3:-113354 | None:intergenic |
| AGTGTGGGGCCTCCACGTAG+GGG | 0.652232 | 5.3:-120292 | None:intergenic |
| CCATTGGCTACATATACGCA+AGG | 0.657826 | 5.3:+113354 | MS.gene071680:CDS |
| CAGACCTGCTCCAACCAGCA+AGG | 0.663412 | 5.3:+120373 | MS.gene071680:CDS |
| AGAAACAACTCAGTTCAGAA+GGG | 0.671630 | 5.3:+119590 | MS.gene071680:CDS |
| AATGTAGAGCCCCTACGTGG+AGG | 0.675024 | 5.3:+120283 | MS.gene071680:intron |
| CATCAAATCCCAAGTTACAG+CGG | 0.686887 | 5.3:+113460 | MS.gene071680:CDS |
| ATGAATGTAGAGCCCCTACG+TGG | 0.707929 | 5.3:+120280 | MS.gene071680:intron |
| TTGCATGCCTGCAAAGTGTG+GGG | 0.711237 | 5.3:-120306 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 111338 | 120400 | 111338 | ID=MS.gene071680 |
| chr5.3 | mRNA | 111338 | 120400 | 111338 | ID=MS.gene071680.t1;Parent=MS.gene071680 |
| chr5.3 | exon | 111338 | 111423 | 111338 | ID=MS.gene071680.t1.exon1;Parent=MS.gene071680.t1 |
| chr5.3 | CDS | 111338 | 111423 | 111338 | ID=cds.MS.gene071680.t1;Parent=MS.gene071680.t1 |
| chr5.3 | exon | 111552 | 111606 | 111552 | ID=MS.gene071680.t1.exon2;Parent=MS.gene071680.t1 |
| chr5.3 | CDS | 111552 | 111606 | 111552 | ID=cds.MS.gene071680.t1;Parent=MS.gene071680.t1 |
| chr5.3 | exon | 111719 | 111801 | 111719 | ID=MS.gene071680.t1.exon3;Parent=MS.gene071680.t1 |
| chr5.3 | CDS | 111719 | 111801 | 111719 | ID=cds.MS.gene071680.t1;Parent=MS.gene071680.t1 |
| chr5.3 | exon | 111889 | 112048 | 111889 | ID=MS.gene071680.t1.exon4;Parent=MS.gene071680.t1 |
| chr5.3 | CDS | 111889 | 112048 | 111889 | ID=cds.MS.gene071680.t1;Parent=MS.gene071680.t1 |
| chr5.3 | exon | 112881 | 113010 | 112881 | ID=MS.gene071680.t1.exon5;Parent=MS.gene071680.t1 |
| chr5.3 | CDS | 112881 | 113010 | 112881 | ID=cds.MS.gene071680.t1;Parent=MS.gene071680.t1 |
| chr5.3 | exon | 113330 | 113488 | 113330 | ID=MS.gene071680.t1.exon6;Parent=MS.gene071680.t1 |
| chr5.3 | CDS | 113330 | 113488 | 113330 | ID=cds.MS.gene071680.t1;Parent=MS.gene071680.t1 |
| chr5.3 | exon | 119554 | 119732 | 119554 | ID=MS.gene071680.t1.exon7;Parent=MS.gene071680.t1 |
| chr5.3 | CDS | 119554 | 119732 | 119554 | ID=cds.MS.gene071680.t1;Parent=MS.gene071680.t1 |
| chr5.3 | exon | 119828 | 119908 | 119828 | ID=MS.gene071680.t1.exon8;Parent=MS.gene071680.t1 |
| chr5.3 | CDS | 119828 | 119908 | 119828 | ID=cds.MS.gene071680.t1;Parent=MS.gene071680.t1 |
| chr5.3 | exon | 119985 | 120140 | 119985 | ID=MS.gene071680.t1.exon9;Parent=MS.gene071680.t1 |
| chr5.3 | CDS | 119985 | 120140 | 119985 | ID=cds.MS.gene071680.t1;Parent=MS.gene071680.t1 |
| chr5.3 | exon | 120290 | 120400 | 120290 | ID=MS.gene071680.t1.exon10;Parent=MS.gene071680.t1 |
| chr5.3 | CDS | 120290 | 120400 | 120290 | ID=cds.MS.gene071680.t1;Parent=MS.gene071680.t1 |
| Gene Sequence |
| Protein sequence |
