AlfalfaGEDB | MS.gene072352

M. sativa cultivar XinJiangDaYe / MS.gene072352

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF01428.19 PF01754.19

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072352.t1	XP_024626996.1	95.9	172	6	1	1	171	1	172	1.70E-89	338.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072352.t1	A2YEZ6	59.6	171	64	2	4	171	3	171	5.3e-55	215.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072352.t1	G7KUE5	95.9	172	6	1	1	171	89	260	1.2e-89	338.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050380	MS.gene072352	0.810636	1.04E-50	-1.69E-46
MS.gene054003	MS.gene072352	-0.805078	1.59E-49	-1.69E-46
MS.gene054486	MS.gene072352	-0.822971	1.78E-53	-1.69E-46
MS.gene056647	MS.gene072352	0.800419	1.45E-48	-1.69E-46
MS.gene057173	MS.gene072352	0.855054	8.16E-62	-1.69E-46
MS.gene058157	MS.gene072352	-0.814644	1.39E-51	-1.69E-46
MS.gene060482	MS.gene072352	-0.800609	1.33E-48	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072352.t1	MTR_7g092400	95.930	172	6	1	1	171	89	260	1.79e-121	343
MS.gene072352.t1	MTR_0249s0070	72.093	172	44	2	1	171	9	177	2.91e-89	258
MS.gene072352.t1	MTR_0249s0070	72.093	172	44	2	1	171	1	169	2.92e-89	258
MS.gene072352.t1	MTR_2g098160	60.000	170	66	2	4	171	3	172	2.09e-72	216
MS.gene072352.t1	MTR_4g065570	59.859	142	51	2	31	171	1	137	5.06e-57	175
MS.gene072352.t1	MTR_7g104320	40.341	176	86	3	5	171	3	168	5.14e-43	141
MS.gene072352.t1	MTR_2g086190	41.401	157	76	3	15	171	10	150	2.42e-38	128
MS.gene072352.t1	MTR_1g060380	42.604	169	80	2	5	171	3	156	4.41e-38	128
MS.gene072352.t1	MTR_4g053440	39.535	172	95	4	5	171	3	170	7.70e-37	125
MS.gene072352.t1	MTR_8g036980	61.765	68	26	0	104	171	84	151	1.74e-27	100
MS.gene072352.t1	MTR_1g100773	36.774	155	79	4	15	169	3	138	5.02e-27	99.4
MS.gene072352.t1	MTR_3g028010	37.419	155	78	2	17	171	28	163	1.18e-26	99.4
MS.gene072352.t1	MTR_3g025570	89.362	47	5	0	103	149	1	47	2.41e-26	97.1
MS.gene072352.t1	MTR_7g114920	33.766	154	76	3	15	168	3	130	1.22e-24	93.2
MS.gene072352.t1	MTR_2g054650	30.968	155	74	3	15	169	4	125	9.42e-18	75.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072352.t1	AT1G51200	53.073	179	65	4	4	171	3	173	1.37e-51	163
MS.gene072352.t1	AT1G51200	53.073	179	65	4	4	171	3	173	1.37e-51	163
MS.gene072352.t1	AT1G51200	53.073	179	65	4	4	171	3	173	1.37e-51	163
MS.gene072352.t1	AT1G51200	53.073	179	65	4	4	171	3	173	1.37e-51	163
MS.gene072352.t1	AT2G36320	44.048	168	84	2	5	171	3	161	7.51e-45	145
MS.gene072352.t1	AT3G52800	42.690	171	91	3	5	171	3	170	1.69e-39	132
MS.gene072352.t1	AT3G52800	42.690	171	91	3	5	171	3	170	1.69e-39	132
MS.gene072352.t1	AT2G27580	37.500	168	84	3	13	171	8	163	1.70e-37	127
MS.gene072352.t1	AT2G27580	37.500	168	84	3	13	171	8	163	1.70e-37	127
MS.gene072352.t1	AT4G12040	36.310	168	94	3	12	171	13	175	2.21e-36	124
MS.gene072352.t1	AT4G12040	36.310	168	94	3	12	171	13	175	2.21e-36	124
MS.gene072352.t1	AT1G12440	38.788	165	89	2	11	171	12	168	2.46e-36	124
MS.gene072352.t1	AT1G12440	38.788	165	89	2	11	171	12	168	2.46e-36	124
MS.gene072352.t1	AT1G12440	38.788	165	89	2	11	171	12	168	2.46e-36	124
MS.gene072352.t1	AT4G22820	38.750	160	93	3	15	171	19	176	1.34e-34	120
MS.gene072352.t1	AT4G22820	38.750	160	93	3	15	171	19	176	1.34e-34	120
MS.gene072352.t1	AT3G12630	38.926	149	72	3	23	171	31	160	9.31e-31	109
MS.gene072352.t1	AT4G14225	35.032	157	66	4	15	171	5	125	4.44e-26	96.7
MS.gene072352.t1	AT4G25380	31.013	158	72	3	16	171	8	130	1.29e-24	93.2

Find 44 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAGTTAATCCAACACGTTTC+CGG	0.268508	7.2:+16748425	None:intergenic
AGTTTATCCGCCTTAATTAC+TGG	0.271098	7.2:+16748289	None:intergenic
CGGAAACGTGTTGGATTAAC+TGG	0.273573	7.2:-16748424	MS.gene072352:CDS
TGAATCCAACCCAGTAATTA+AGG	0.350084	7.2:-16748299	MS.gene072352:CDS
CAATTATTAACGCACAGAAT+TGG	0.359796	7.2:+16748727	None:intergenic
ATCTCCATCTCATTAGAGAT+TGG	0.379259	7.2:+16748484	None:intergenic
CCAGCAGATGCACTACTTGC+CGG	0.396434	7.2:-16748444	MS.gene072352:CDS
GAATTGGACGTTCAGGTGTC+TGG	0.402600	7.2:+16748743	None:intergenic
GTTAATAATTGTGGTTTCTT+CGG	0.432078	7.2:-16748715	MS.gene072352:CDS
GCATCTGCTGGGACCAGTCT+TGG	0.436198	7.2:+16748456	None:intergenic
CCGGCAAGTAGTGCATCTGC+TGG	0.450708	7.2:+16748444	None:intergenic
ACGCACAGAATTGGACGTTC+AGG	0.451830	7.2:+16748736	None:intergenic
CCAGCAGTTCTATAATCAAA+AGG	0.452505	7.2:+16748337	None:intergenic
ATCCGCCTTAATTACTGGGT+TGG	0.457754	7.2:+16748294	None:intergenic
TTCCAGCGGCAATGGAAAGC+TGG	0.468107	7.2:-16748584	MS.gene072352:CDS
AGCCAGCTTTCCATTGCCGC+TGG	0.484638	7.2:+16748582	None:intergenic
AATGGCAATTCCAGCGGCAA+TGG	0.485507	7.2:-16748592	MS.gene072352:CDS
GTTTATCCGCCTTAATTACT+GGG	0.487636	7.2:+16748290	None:intergenic
CATGTGTTCCAAGTGTTACA+AGG	0.489662	7.2:-16748674	MS.gene072352:CDS
ACACTTGGAACACATGTTCA+TGG	0.501814	7.2:+16748681	None:intergenic
TACTGGGTTGGATTCAGCTA+TGG	0.503220	7.2:+16748306	None:intergenic
AATGGAGTCTCATGATGAGA+TGG	0.509058	7.2:-16748770	MS.gene072352:CDS
ATAATTGTGGTTTCTTCGGA+AGG	0.510786	7.2:-16748711	MS.gene072352:CDS
ATTCTGTGCGTTAATAATTG+TGG	0.511147	7.2:-16748724	MS.gene072352:CDS
CAGCAGTTCTATAATCAAAA+GGG	0.523968	7.2:+16748338	None:intergenic
CAGTGTGTCCTTGTAACACT+TGG	0.534504	7.2:+16748666	None:intergenic
AGCGATGCATTGAGCAAAAG+AGG	0.535711	7.2:+16748380	None:intergenic
TCAGTTGAAAACATCGTGAA+TGG	0.542840	7.2:-16748610	MS.gene072352:CDS
CAAGGACACACTGCTGAAGC+AGG	0.543963	7.2:-16748656	MS.gene072352:CDS
ATGGAGATGAAAGCCAAGAC+TGG	0.550708	7.2:-16748469	MS.gene072352:CDS
AAATCCAATCTCTAATGAGA+TGG	0.551991	7.2:-16748488	MS.gene072352:CDS
CACACTGCTGAAGCAGGAGC+AGG	0.565527	7.2:-16748650	MS.gene072352:CDS
CGGCAAGTAGTGCATCTGCT+GGG	0.567957	7.2:+16748445	None:intergenic
ACTGGTTTCAGCTGCAAATG+TGG	0.568603	7.2:-16748406	MS.gene072352:CDS
TTATAGAACTGCTGGTCAGA+AGG	0.572119	7.2:-16748329	MS.gene072352:CDS
GAAAGCTGGCTGTGACCGCA+AGG	0.589655	7.2:-16748570	MS.gene072352:CDS
ACTACTTGCCGGAAACGTGT+TGG	0.596630	7.2:-16748433	MS.gene072352:CDS
TAATTGTGGTTTCTTCGGAA+GGG	0.609724	7.2:-16748710	MS.gene072352:CDS
ATGGAGTCTCATGATGAGAT+GGG	0.645569	7.2:-16748769	MS.gene072352:CDS
ATCGTGAATGGCAATTCCAG+CGG	0.654212	7.2:-16748598	MS.gene072352:CDS
TCGCACATCAACAGTCCTTG+CGG	0.667104	7.2:+16748555	None:intergenic
GCTCAATACAGTGTCTCCCG+AGG	0.676644	7.2:-16748518	MS.gene072352:CDS
ATCCAACCCAGTAATTAAGG+CGG	0.681546	7.2:-16748296	MS.gene072352:CDS
TGATGTGCGAGTTGAAAGTG+TGG	0.723729	7.2:-16748542	MS.gene072352:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GTTAATAATTGTGGTTTCTT+CGG	-	chr7.2:16748330-16748349	MS.gene072352:CDS	25.0%
	AAATCCAATCTCTAATGAGA+TGG	-	chr7.2:16748557-16748576	MS.gene072352:CDS	30.0%
	ATTCTGTGCGTTAATAATTG+TGG	-	chr7.2:16748321-16748340	MS.gene072352:CDS	30.0%
	CAATTATTAACGCACAGAAT+TGG	+	chr7.2:16748321-16748340	None:intergenic	30.0%
	CAGCAGTTCTATAATCAAAA+GGG	+	chr7.2:16748710-16748729	None:intergenic	30.0%
!	TCATTAGAGATTGGATTTTC+TGG	+	chr7.2:16748555-16748574	None:intergenic	30.0%
	AGTTTATCCGCCTTAATTAC+TGG	+	chr7.2:16748759-16748778	None:intergenic	35.0%
	ATAATTGTGGTTTCTTCGGA+AGG	-	chr7.2:16748334-16748353	MS.gene072352:CDS	35.0%
	ATCTCCATCTCATTAGAGAT+TGG	+	chr7.2:16748564-16748583	None:intergenic	35.0%
	CCAGCAGTTCTATAATCAAA+AGG	+	chr7.2:16748711-16748730	None:intergenic	35.0%
	GTTTATCCGCCTTAATTACT+GGG	+	chr7.2:16748758-16748777	None:intergenic	35.0%
	TAATTGTGGTTTCTTCGGAA+GGG	-	chr7.2:16748335-16748354	MS.gene072352:CDS	35.0%
	TCAGTTGAAAACATCGTGAA+TGG	-	chr7.2:16748435-16748454	MS.gene072352:CDS	35.0%
	TGAATCCAACCCAGTAATTA+AGG	-	chr7.2:16748746-16748765	MS.gene072352:CDS	35.0%
!!	CCTTTTGATTATAGAACTGC+TGG	-	chr7.2:16748708-16748727	MS.gene072352:CDS	35.0%
	ACACTTGGAACACATGTTCA+TGG	+	chr7.2:16748367-16748386	None:intergenic	40.0%
	ATCCAACCCAGTAATTAAGG+CGG	-	chr7.2:16748749-16748768	MS.gene072352:CDS	40.0%
	ATGGAGTCTCATGATGAGAT+GGG	-	chr7.2:16748276-16748295	MS.gene072352:CDS	40.0%
	CAGTTAATCCAACACGTTTC+CGG	+	chr7.2:16748623-16748642	None:intergenic	40.0%
	CATGTGTTCCAAGTGTTACA+AGG	-	chr7.2:16748371-16748390	MS.gene072352:CDS	40.0%
	TTATAGAACTGCTGGTCAGA+AGG	-	chr7.2:16748716-16748735	MS.gene072352:CDS	40.0%
!!!	ATTGGATTTTCTGGAACCTC+GGG	+	chr7.2:16748546-16748565	None:intergenic	40.0%
!!!	GATTGGATTTTCTGGAACCT+CGG	+	chr7.2:16748547-16748566	None:intergenic	40.0%
	AGCGATGCATTGAGCAAAAG+AGG	+	chr7.2:16748668-16748687	None:intergenic	45.0%
	ATCCGCCTTAATTACTGGGT+TGG	+	chr7.2:16748754-16748773	None:intergenic	45.0%
	ATCGTGAATGGCAATTCCAG+CGG	-	chr7.2:16748447-16748466	MS.gene072352:CDS	45.0%
	ATGGAGATGAAAGCCAAGAC+TGG	-	chr7.2:16748576-16748595	MS.gene072352:CDS	45.0%
	CAGTGTGTCCTTGTAACACT+TGG	+	chr7.2:16748382-16748401	None:intergenic	45.0%
	CGGAAACGTGTTGGATTAAC+TGG	-	chr7.2:16748621-16748640	MS.gene072352:CDS	45.0%
!	ACTGGTTTCAGCTGCAAATG+TGG	-	chr7.2:16748639-16748658	MS.gene072352:CDS	45.0%
!	TACTGGGTTGGATTCAGCTA+TGG	+	chr7.2:16748742-16748761	None:intergenic	45.0%
!	TGATGTGCGAGTTGAAAGTG+TGG	-	chr7.2:16748503-16748522	MS.gene072352:CDS	45.0%
	AATGGCAATTCCAGCGGCAA+TGG	-	chr7.2:16748453-16748472	MS.gene072352:CDS	50.0%
	ACGCACAGAATTGGACGTTC+AGG	+	chr7.2:16748312-16748331	None:intergenic	50.0%
	ACTACTTGCCGGAAACGTGT+TGG	-	chr7.2:16748612-16748631	MS.gene072352:CDS	50.0%
	GAATTGGACGTTCAGGTGTC+TGG	+	chr7.2:16748305-16748324	None:intergenic	50.0%
	TCGCACATCAACAGTCCTTG+CGG	+	chr7.2:16748493-16748512	None:intergenic	50.0%
	CAAGGACACACTGCTGAAGC+AGG	-	chr7.2:16748389-16748408	MS.gene072352:CDS	55.0%
	CCAGCAGATGCACTACTTGC+CGG	-	chr7.2:16748601-16748620	MS.gene072352:CDS	55.0%
	CGGCAAGTAGTGCATCTGCT+GGG	+	chr7.2:16748603-16748622	None:intergenic	55.0%
	GCTCAATACAGTGTCTCCCG+AGG	-	chr7.2:16748527-16748546	MS.gene072352:CDS	55.0%
	TTCCAGCGGCAATGGAAAGC+TGG	-	chr7.2:16748461-16748480	MS.gene072352:CDS	55.0%
	AGCCAGCTTTCCATTGCCGC+TGG	+	chr7.2:16748466-16748485	None:intergenic	60.0%
	CACACTGCTGAAGCAGGAGC+AGG	-	chr7.2:16748395-16748414	MS.gene072352:CDS	60.0%
	CCGGCAAGTAGTGCATCTGC+TGG	+	chr7.2:16748604-16748623	None:intergenic	60.0%
	GAAAGCTGGCTGTGACCGCA+AGG	-	chr7.2:16748475-16748494	MS.gene072352:CDS	60.0%
	GCATCTGCTGGGACCAGTCT+TGG	+	chr7.2:16748592-16748611	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	16748276	16748791	16748276	ID=MS.gene072352
chr7.2	mRNA	16748276	16748791	16748276	ID=MS.gene072352.t1;Parent=MS.gene072352
chr7.2	exon	16748276	16748791	16748276	ID=MS.gene072352.t1.exon1;Parent=MS.gene072352.t1
chr7.2	CDS	16748276	16748791	16748276	ID=cds.MS.gene072352.t1;Parent=MS.gene072352.t1

Gene Sequence

>MS.gene072352

ATGGAGTCTCATGATGAGATGGGATGCCAGACACCTGAACGTCCAATTCTGTGCGTTAATAATTGTGGTTTCTTCGGAAGGGAAGCTACCATGAACATGTGTTCCAAGTGTTACAAGGACACACTGCTGAAGCAGGAGCAGGAAAAACTTGTTGCGACGTCAGTTGAAAACATCGTGAATGGCAATTCCAGCGGCAATGGAAAGCTGGCTGTGACCGCAAGGACTGTTGATGTGCGAGTTGAAAGTGTGGAGCTCAATACAGTGTCTCCCGAGGTTCCAGAAAATCCAATCTCTAATGAGATGGAGATGAAAGCCAAGACTGGTCCCAGCAGATGCACTACTTGCCGGAAACGTGTTGGATTAACTGGTTTCAGCTGCAAATGTGGCAACCTCTTTTGCTCAATGCATCGCTATTCTGATAAACACGATTGCCCTTTTGATTATAGAACTGCTGGTCAGAAGGCCATAGCTGAATCCAACCCAGTAATTAAGGCGGATAAACTCGATAAAATCTAG

Protein sequence

>MS.gene072352.t1

MESHDEMGCQTPERPILCVNNCGFFGREATMNMCSKCYKDTLLKQEQEKLVATSVENIVNGNSSGNGKLAVTARTVDVRVESVELNTVSPEVPENPISNEMEMKAKTGPSRCTTCRKRVGLTGFSCKCGNLFCSMHRYSDKHDCPFDYRTAGQKAIAESNPVIKADKLDKI