Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072402.t1 | PNX82677.1 | 67.8 | 118 | 36 | 2 | 25 | 140 | 24 | 141 | 3.60E-29 | 137.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072402.t1 | A0A2K3LVW6 | 67.8 | 118 | 36 | 2 | 25 | 140 | 24 | 141 | 2.6e-29 | 137.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049418 | MS.gene072402 | 0.809107 | 2.23E-50 | -1.69E-46 |
| MS.gene049989 | MS.gene072402 | 0.847702 | 9.78E-60 | -1.69E-46 |
| MS.gene050297 | MS.gene072402 | 0.834278 | 3.29E-56 | -1.69E-46 |
| MS.gene050310 | MS.gene072402 | 0.81871 | 1.70E-52 | -1.69E-46 |
| MS.gene050648 | MS.gene072402 | 0.832909 | 7.22E-56 | -1.69E-46 |
| MS.gene051067 | MS.gene072402 | 0.800437 | 1.44E-48 | -1.69E-46 |
| MS.gene051263 | MS.gene072402 | 0.839009 | 2.05E-57 | -1.69E-46 |
| MS.gene051478 | MS.gene072402 | 0.837856 | 4.06E-57 | -1.69E-46 |
| MS.gene051629 | MS.gene072402 | 0.806661 | 7.38E-50 | -1.69E-46 |
| MS.gene053929 | MS.gene072402 | 0.81519 | 1.05E-51 | -1.69E-46 |
| MS.gene055957 | MS.gene072402 | 0.810281 | 1.25E-50 | -1.69E-46 |
| MS.gene056042 | MS.gene072402 | 0.807603 | 4.66E-50 | -1.69E-46 |
| MS.gene056169 | MS.gene072402 | 0.818776 | 1.64E-52 | -1.69E-46 |
| MS.gene056228 | MS.gene072402 | 0.841359 | 4.99E-58 | -1.69E-46 |
| MS.gene057004 | MS.gene072402 | 0.82475 | 6.82E-54 | -1.69E-46 |
| MS.gene058196 | MS.gene072402 | 0.822894 | 1.86E-53 | -1.69E-46 |
| MS.gene058197 | MS.gene072402 | 0.860779 | 1.63E-63 | -1.69E-46 |
| MS.gene058198 | MS.gene072402 | 0.867539 | 1.27E-65 | -1.69E-46 |
| MS.gene059300 | MS.gene072402 | 0.840124 | 1.05E-57 | -1.69E-46 |
| MS.gene06057 | MS.gene072402 | 0.886294 | 3.88E-72 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072402.t1 | MTR_7g093220 | 99.153 | 118 | 1 | 0 | 23 | 140 | 22 | 139 | 5.55e-81 | 234 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072402.t1 | AT2G42110 | 48.062 | 129 | 59 | 4 | 19 | 140 | 10 | 137 | 1.05e-26 | 97.4 |
Find 0 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GGTAATTTAGATTTATTTAA+CGG | - | 51337:133906-133925 | None:intergenic | 15.0% |
| !!! | ACCAAGAAAAAGTGTTAATT+GGG | - | 51337:133853-133872 | None:intergenic | 25.0% |
| TTGTATAGATTAATTCGTCG+AGG | - | 51337:133927-133946 | None:intergenic | 30.0% | |
| ! | GATTTTCCTTCATCTTTTTG+CGG | - | 51337:133677-133696 | None:intergenic | 30.0% |
| !! | CCAAGAAAAAGTGTTAATTG+GGG | - | 51337:133852-133871 | None:intergenic | 30.0% |
| !! | GACCAAGAAAAAGTGTTAAT+TGG | - | 51337:133854-133873 | None:intergenic | 30.0% |
| ACGTAACTCTTTCTTCTCAT+TGG | - | 51337:133643-133662 | None:intergenic | 35.0% | |
| ACTATACTTTGTTGCAGACA+TGG | + | 51337:134001-134020 | MS.gene072402:intron | 35.0% | |
| AGAAACAAGTCATCACTCTT+GGG | + | 51337:133807-133826 | MS.gene072402:CDS | 35.0% | |
| CATTGATCAAACGCTTATGA+CGG | - | 51337:134077-134096 | None:intergenic | 35.0% | |
| TACTGTTACTACTGATGCTA+AGG | + | 51337:133557-133576 | MS.gene072402:CDS | 35.0% | |
| ! | CTTTTCCATGAAAACTCTAG+AGG | - | 51337:134046-134065 | None:intergenic | 35.0% |
| !!! | CCCCAATTAACACTTTTTCT+TGG | + | 51337:133849-133868 | MS.gene072402:intron | 35.0% |
| AATCTTCTGATGAGCTACTC+AGG | + | 51337:133598-133617 | MS.gene072402:CDS | 40.0% | |
| AGTAGAAGTAGTAGCAGAAG+TGG | - | 51337:133541-133560 | None:intergenic | 40.0% | |
| CAATCTGAGAGCTCTTCAAA+CGG | + | 51337:133696-133715 | MS.gene072402:CDS | 40.0% | |
| CAGAAACAAGTCATCACTCT+TGG | + | 51337:133806-133825 | MS.gene072402:CDS | 40.0% | |
| ACATGGCGTAGAACTGTAGA+AGG | + | 51337:134018-134037 | MS.gene072402:CDS | 45.0% | |
| ACTCTTGGGAACCATTCACA+AGG | + | 51337:133821-133840 | MS.gene072402:CDS | 45.0% | |
| CAAACGCCGCAAAAAGATGA+AGG | + | 51337:133668-133687 | MS.gene072402:CDS | 45.0% | |
| !! | ACTCAGGAAGTTTGCTGAAG+TGG | + | 51337:133614-133633 | MS.gene072402:CDS | 45.0% |
| !! | CTCAGGAAGTTTGCTGAAGT+GGG | + | 51337:133615-133634 | MS.gene072402:CDS | 45.0% |
| !!! | AGGTGCCTCTAGAGTTTTCA+TGG | + | 51337:134038-134057 | MS.gene072402:CDS | 45.0% |
| AAGCGACCTCCTTTCCACAA+CGG | - | 51337:133730-133749 | None:intergenic | 50.0% | |
| TATCTCTAACCCTGAGCTGC+CGG | - | 51337:133785-133804 | None:intergenic | 50.0% | |
| TTGGGGTCTCACCTTGTGAA+TGG | - | 51337:133835-133854 | None:intergenic | 50.0% | |
| ! | TTTTACCGGCAACAGTGAGC+CGG | + | 51337:133748-133767 | MS.gene072402:CDS | 50.0% |
| !!! | GGAAAGGAGGTCGCTTTTAC+CGG | + | 51337:133734-133753 | MS.gene072402:CDS | 50.0% |
| AAACGGCACCACTGCCGTTG+TGG | + | 51337:133713-133732 | MS.gene072402:CDS | 60.0% | |
| CCACTGCCGTTGTGGAAAGG+AGG | + | 51337:133721-133740 | MS.gene072402:CDS | 60.0% | |
| CCTCCTTTCCACAACGGCAG+TGG | - | 51337:133724-133743 | None:intergenic | 60.0% | |
| GACTTCCGGCTCACTGTTGC+CGG | - | 51337:133756-133775 | None:intergenic | 60.0% | |
| GCACCACTGCCGTTGTGGAA+AGG | + | 51337:133718-133737 | MS.gene072402:CDS | 60.0% | |
| TGAGCCGGAAGTCAGTGCTC+CGG | + | 51337:133763-133782 | MS.gene072402:CDS | 60.0% | |
| AGTCAGTGCTCCGGCAGCTC+AGG | + | 51337:133772-133791 | MS.gene072402:CDS | 65.0% | |
| GTCAGTGCTCCGGCAGCTCA+GGG | + | 51337:133773-133792 | MS.gene072402:CDS | 65.0% | |
| !! | GCTGCCGGAGCACTGACTTC+CGG | - | 51337:133770-133789 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 51337 | gene | 133510 | 134107 | 133510 | ID=MS.gene072402 |
| 51337 | mRNA | 133510 | 134107 | 133510 | ID=MS.gene072402.t1;Parent=MS.gene072402 |
| 51337 | exon | 133510 | 133842 | 133510 | ID=MS.gene072402.t1.exon1;Parent=MS.gene072402.t1 |
| 51337 | CDS | 133510 | 133842 | 133510 | ID=cds.MS.gene072402.t1;Parent=MS.gene072402.t1 |
| 51337 | exon | 134018 | 134107 | 134018 | ID=MS.gene072402.t1.exon2;Parent=MS.gene072402.t1 |
| 51337 | CDS | 134018 | 134107 | 134018 | ID=cds.MS.gene072402.t1;Parent=MS.gene072402.t1 |
| Gene Sequence |
| Protein sequence |
