Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072595.t1 | XP_003626145.1 | 97.8 | 455 | 8 | 2 | 1 | 453 | 1 | 455 | 1.30E-261 | 911.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072595.t1 | Q3E6S9 | 71.9 | 430 | 121 | 0 | 23 | 452 | 45 | 474 | 2.7e-191 | 669.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072595.t1 | G7KWF1 | 97.8 | 455 | 8 | 2 | 1 | 453 | 1 | 455 | 9.4e-262 | 911.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050796 | MS.gene072595 | 0.810799 | 9.63E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene20906 | MS.gene072595 | PPI |
| MS.gene07363 | MS.gene072595 | PPI |
| MS.gene20907 | MS.gene072595 | PPI |
| MS.gene005429 | MS.gene072595 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072595.t1 | MTR_7g111540 | 97.802 | 455 | 8 | 2 | 1 | 453 | 1 | 455 | 0.0 | 928 |
| MS.gene072595.t1 | MTR_1g484920 | 71.018 | 452 | 116 | 7 | 4 | 452 | 5 | 444 | 0.0 | 668 |
| MS.gene072595.t1 | MTR_1g484920 | 63.778 | 450 | 109 | 7 | 4 | 452 | 5 | 401 | 0.0 | 582 |
| MS.gene072595.t1 | MTR_1g086070 | 60.000 | 445 | 167 | 4 | 16 | 453 | 9 | 449 | 0.0 | 559 |
| MS.gene072595.t1 | MTR_3g083980 | 24.291 | 247 | 157 | 9 | 52 | 286 | 79 | 307 | 1.31e-11 | 66.2 |
| MS.gene072595.t1 | MTR_3g083980 | 24.291 | 247 | 157 | 9 | 52 | 286 | 79 | 307 | 2.35e-11 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072595.t1 | AT5G26600 | 71.860 | 430 | 121 | 0 | 23 | 452 | 45 | 474 | 0.0 | 660 |
| MS.gene072595.t1 | AT5G26600 | 71.860 | 430 | 121 | 0 | 23 | 452 | 45 | 474 | 0.0 | 660 |
| MS.gene072595.t1 | AT3G62130 | 59.688 | 449 | 170 | 3 | 10 | 451 | 7 | 451 | 0.0 | 585 |
| MS.gene072595.t1 | AT3G62130 | 59.688 | 449 | 170 | 3 | 10 | 451 | 7 | 451 | 0.0 | 585 |
Find 0 sgRNAs with CRISPR-Local
Find 141 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATTGGAAGAAGATAGTTT+AGG | + | 50590:24981-25000 | None:intergenic | 25.0% |
| ! | ACTAAAAAGAATCCTAAAAC+TGG | - | 50590:25728-25747 | MS.gene072595:CDS | 25.0% |
| ! | TTCTTATCAGGTTTACAATA+AGG | - | 50590:26165-26184 | MS.gene072595:CDS | 25.0% |
| !! | ATACTAGTAATTTGCATAAG+TGG | - | 50590:25672-25691 | MS.gene072595:CDS | 25.0% |
| !!! | AAGATAGTTTAGGTTTTTTG+GGG | + | 50590:24971-24990 | None:intergenic | 25.0% |
| !!! | AGAAGATAGTTTAGGTTTTT+TGG | + | 50590:24973-24992 | None:intergenic | 25.0% |
| !!! | GAAGATAGTTTAGGTTTTTT+GGG | + | 50590:24972-24991 | None:intergenic | 25.0% |
| !!! | TTAGAAAAGCTTTGGAAAAA+GGG | - | 50590:25462-25481 | MS.gene072595:CDS | 25.0% |
| !!! | TTTAGAAAAGCTTTGGAAAA+AGG | - | 50590:25461-25480 | MS.gene072595:CDS | 25.0% |
| AAAAAGAATCCTAAAACTGG+TGG | - | 50590:25731-25750 | MS.gene072595:CDS | 30.0% | |
| ATACTAGCACACATATGTTT+CGG | + | 50590:25986-26005 | None:intergenic | 30.0% | |
| CTTTGTTGAATTTACCTTCA+CGG | + | 50590:25301-25320 | None:intergenic | 30.0% | |
| GACTAAGTTTAGAAAAGCTT+TGG | - | 50590:25454-25473 | MS.gene072595:CDS | 30.0% | |
| GATGTTGATATGCAAGATAT+TGG | - | 50590:25638-25657 | MS.gene072595:CDS | 30.0% | |
| GTTATGCTTCATTATGCTTA+CGG | - | 50590:25332-25351 | MS.gene072595:CDS | 30.0% | |
| TGGGAAGAAAATTAGATTAG+CGG | - | 50590:25493-25512 | MS.gene072595:CDS | 30.0% | |
| TTGAGGACTATTACAAGTTT+CGG | - | 50590:26188-26207 | MS.gene072595:CDS | 30.0% | |
| ! | GATTTTGTGAATAGGTTTGA+AGG | - | 50590:25869-25888 | MS.gene072595:CDS | 30.0% |
| !!! | AGATAGTTTAGGTTTTTTGG+GGG | + | 50590:24970-24989 | None:intergenic | 30.0% |
| AAGGGATTATAGTGCTCAAT+TGG | - | 50590:25829-25848 | MS.gene072595:CDS | 35.0% | |
| ATCTGAATCAATTCCTTCAC+AGG | + | 50590:25557-25576 | None:intergenic | 35.0% | |
| CATGAAGCTGTTATTGAGAT+GGG | - | 50590:25920-25939 | MS.gene072595:CDS | 35.0% | |
| CGTGAAGGTAAATTCAACAA+AGG | - | 50590:25299-25318 | MS.gene072595:CDS | 35.0% | |
| CTAGGCGGTCTATAATATAT+AGG | + | 50590:26106-26125 | None:intergenic | 35.0% | |
| GAAACATATGTGTGCTAGTA+TGG | - | 50590:25985-26004 | MS.gene072595:CDS | 35.0% | |
| GTATGCAAGGATTTCTTATC+AGG | - | 50590:26153-26172 | MS.gene072595:CDS | 35.0% | |
| TATGTGTGCTAGTATGGTTA+TGG | - | 50590:25991-26010 | MS.gene072595:CDS | 35.0% | |
| TCAGGTTTACAATAAGGTTG+AGG | - | 50590:26171-26190 | MS.gene072595:CDS | 35.0% | |
| TCATGAAGCTGTTATTGAGA+TGG | - | 50590:25919-25938 | MS.gene072595:CDS | 35.0% | |
| TGAGGACTATTACAAGTTTC+GGG | - | 50590:26189-26208 | MS.gene072595:CDS | 35.0% | |
| TTGGAAAAAGGGAAAGTTGA+TGG | - | 50590:25473-25492 | MS.gene072595:CDS | 35.0% | |
| TTGTGAATAGGTTTGAAGGA+GGG | - | 50590:25873-25892 | MS.gene072595:CDS | 35.0% | |
| TTTGTGAATAGGTTTGAAGG+AGG | - | 50590:25872-25891 | MS.gene072595:CDS | 35.0% | |
| ! | ACACATTTGAGGGATGTTTT+TGG | - | 50590:26070-26089 | MS.gene072595:CDS | 35.0% |
| ! | ATTGATTCAGATTTGCAGAG+AGG | - | 50590:25565-25584 | MS.gene072595:CDS | 35.0% |
| ! | TGGAAAAAGGGAAAGTTGAT+GGG | - | 50590:25474-25493 | MS.gene072595:CDS | 35.0% |
| ! | TTAACCAACTGGTGGATAAT+GGG | - | 50590:26218-26237 | MS.gene072595:CDS | 35.0% |
| ! | TTCGGATTCAATTTCTGAAG+CGG | + | 50590:25009-25028 | None:intergenic | 35.0% |
| !!! | AGGCTTTGGATTTTGTGAAT+AGG | - | 50590:25861-25880 | MS.gene072595:CDS | 35.0% |
| !!! | CTAGGTTTTGGATAATTGAG+CGG | + | 50590:25190-25209 | None:intergenic | 35.0% |
| !!! | TAGGTTTTGGATAATTGAGC+GGG | + | 50590:25189-25208 | None:intergenic | 35.0% |
| !!! | TTGGTTTGCCTACTTGTTTT+GGG | - | 50590:26014-26033 | MS.gene072595:CDS | 35.0% |
| AAGAATCCTAAAACTGGTGG+TGG | - | 50590:25734-25753 | MS.gene072595:CDS | 40.0% | |
| ATTCAGATTTGCAGAGAGGA+AGG | - | 50590:25569-25588 | MS.gene072595:CDS | 40.0% | |
| ATTCTGATGCACTTAAGCTG+AGG | - | 50590:26047-26066 | MS.gene072595:CDS | 40.0% | |
| CTTCTAACCCCAAAACAAGT+AGG | + | 50590:26025-26044 | None:intergenic | 40.0% | |
| GGATTATAGTGCTCAATTGG+TGG | - | 50590:25832-25851 | MS.gene072595:CDS | 40.0% | |
| GGATTGAAGGGATTAAGAAG+AGG | - | 50590:25894-25913 | MS.gene072595:CDS | 40.0% | |
| GTATACAAAAACGCGATCGA+AGG | + | 50590:25710-25729 | None:intergenic | 40.0% | |
| GTGGTTGAAGTAGAAGTGAT+CGG | + | 50590:25144-25163 | None:intergenic | 40.0% | |
| GTGTCTCATGAGTATGGAAA+TGG | - | 50590:25776-25795 | MS.gene072595:CDS | 40.0% | |
| TAAGCTGAGGACACATTTGA+GGG | - | 50590:26060-26079 | MS.gene072595:CDS | 40.0% | |
| TATTATAGACCGCCTAGAGA+TGG | - | 50590:26109-26128 | MS.gene072595:CDS | 40.0% | |
| TATTGAGATGGGAGAGATGT+TGG | - | 50590:25931-25950 | MS.gene072595:CDS | 40.0% | |
| TGCTGTTAAGAAATCCATGG+AGG | - | 50590:25355-25374 | MS.gene072595:CDS | 40.0% | |
| TGGTTGAAGTAGAAGTGATC+GGG | + | 50590:25143-25162 | None:intergenic | 40.0% | |
| TGTGCTAGTATGGTTATGGT+TGG | - | 50590:25995-26014 | MS.gene072595:CDS | 40.0% | |
| TTAAGCTGAGGACACATTTG+AGG | - | 50590:26059-26078 | MS.gene072595:CDS | 40.0% | |
| TTCACAAAATCCAAAGCCTC+AGG | + | 50590:25860-25879 | None:intergenic | 40.0% | |
| TTCACAGGAATCACAACACA+AGG | + | 50590:25542-25561 | None:intergenic | 40.0% | |
| TTGTGTTGTGATTCCTGTGA+AGG | - | 50590:25541-25560 | MS.gene072595:CDS | 40.0% | |
| ! | AAATCACCACCACCAGTTTT+AGG | + | 50590:25743-25762 | None:intergenic | 40.0% |
| ! | AGAGATGTTGGTGAAAGCTT+GGG | - | 50590:25943-25962 | MS.gene072595:CDS | 40.0% |
| ! | GTTAACCAACTGGTGGATAA+TGG | - | 50590:26217-26236 | MS.gene072595:CDS | 40.0% |
| ! | TGAAGTTCCGTTACCTTTTC+CGG | - | 50590:25409-25428 | MS.gene072595:CDS | 40.0% |
| !! | GTATGGAAATGGATTAGCTG+TGG | - | 50590:25787-25806 | MS.gene072595:CDS | 40.0% |
| !! | GTGGTGATTGTTTTTCTCAG+AGG | + | 50590:24913-24932 | None:intergenic | 40.0% |
| !! | TGATGTCTTTAGCGTTGACT+AGG | + | 50590:25208-25227 | None:intergenic | 40.0% |
| !! | TTCTGAAGCGGTGATGAAAT+TGG | + | 50590:24997-25016 | None:intergenic | 40.0% |
| !!! | AGTTTAGGTTTTTTGGGGGT+GGG | + | 50590:24966-24985 | None:intergenic | 40.0% |
| !!! | CTTTAGCGTTGACTAGGTTT+TGG | + | 50590:25202-25221 | None:intergenic | 40.0% |
| !!! | GTTGGTTTGCCTACTTGTTT+TGG | - | 50590:26013-26032 | MS.gene072595:CDS | 40.0% |
| !!! | TAGTTTAGGTTTTTTGGGGG+TGG | + | 50590:24967-24986 | None:intergenic | 40.0% |
| !!! | TGGTTTGCCTACTTGTTTTG+GGG | - | 50590:26015-26034 | MS.gene072595:CDS | 40.0% |
| AAAGCTTGGGGAACTCATCT+TGG | - | 50590:25956-25975 | MS.gene072595:CDS | 45.0% | |
| AAGCTTGGGGAACTCATCTT+GGG | - | 50590:25957-25976 | MS.gene072595:CDS | 45.0% | |
| AATTGAGCGGGAGTTGTTGA+TGG | + | 50590:25177-25196 | None:intergenic | 45.0% | |
| AGGTTTGAAGGAGGGATTGA+AGG | - | 50590:25881-25900 | MS.gene072595:CDS | 45.0% | |
| ATCTCATCGTTCGAACTCAC+CGG | + | 50590:25431-25450 | None:intergenic | 45.0% | |
| CAAACCCATTATCCACCAGT+TGG | + | 50590:26225-26244 | None:intergenic | 45.0% | |
| CCATACTCATGAGACACAAC+AGG | + | 50590:25773-25792 | None:intergenic | 45.0% | |
| CCTGTTGTGTCTCATGAGTA+TGG | - | 50590:25770-25789 | MS.gene072595:CDS | 45.0% | |
| CGTTCAATCCCAGACATCAA+CGG | - | 50590:24933-24952 | MS.gene072595:CDS | 45.0% | |
| GAACTCACCGGAAAAGGTAA+CGG | + | 50590:25419-25438 | None:intergenic | 45.0% | |
| GGATCAACTTCTCCATCTCT+AGG | + | 50590:26124-26143 | None:intergenic | 45.0% | |
| GGTGTGTTGAAGAACGATAG+CGG | + | 50590:25267-25286 | None:intergenic | 45.0% | |
| GGTTTGAAGGAGGGATTGAA+GGG | - | 50590:25882-25901 | MS.gene072595:CDS | 45.0% | |
| GTTGATGCTGCTCATGCAAT+TGG | - | 50590:25608-25627 | MS.gene072595:CDS | 45.0% | |
| TACAAAAACGCGATCGAAGG+TGG | + | 50590:25707-25726 | None:intergenic | 45.0% | |
| TCAACTTCTCCATCTCTAGG+CGG | + | 50590:26121-26140 | None:intergenic | 45.0% | |
| ! | CGTTGATGTCTGGGATTGAA+CGG | + | 50590:24935-24954 | None:intergenic | 45.0% |
| ! | GAGAGATGTTGGTGAAAGCT+TGG | - | 50590:25942-25961 | MS.gene072595:CDS | 45.0% |
| ! | GAGATGTTGGTGAAAGCTTG+GGG | - | 50590:25944-25963 | MS.gene072595:CDS | 45.0% |
| ! | TGGAGAAGTTGATCCTGTGA+CGG | - | 50590:26129-26148 | MS.gene072595:CDS | 45.0% |
| !! | AAAGTGCTTGGATTGGCACA+AGG | - | 50590:25810-25829 | MS.gene072595:CDS | 45.0% |
| !! | AAGTGCTTGGATTGGCACAA+GGG | - | 50590:25811-25830 | MS.gene072595:CDS | 45.0% |
| !! | CGGTGCTGTTAAGAAATCCA+TGG | - | 50590:25352-25371 | MS.gene072595:CDS | 45.0% |
| !! | GATTAGCTGTGGAAAGTGCT+TGG | - | 50590:25798-25817 | MS.gene072595:CDS | 45.0% |
| !!! | GTTTAGGTTTTTTGGGGGTG+GGG | + | 50590:24965-24984 | None:intergenic | 45.0% |
| ACCTTCACGGAAACACCAAG+CGG | + | 50590:25288-25307 | None:intergenic | 50.0% | |
| CGCATTAACAACGGCAGCTT+TGG | - | 50590:25056-25075 | MS.gene072595:CDS | 50.0% | |
| GGATGCTGTTAACCAACTGG+TGG | - | 50590:26210-26229 | MS.gene072595:CDS | 50.0% | |
| TCCTCTCCGCTCAACAACAA+TGG | - | 50590:25096-25115 | MS.gene072595:CDS | 50.0% | |
| TCGGGATGCTGTTAACCAAC+TGG | - | 50590:26207-26226 | MS.gene072595:CDS | 50.0% | |
| TCGTTCGAACTCACCGGAAA+AGG | + | 50590:25425-25444 | None:intergenic | 50.0% | |
| TCGTTCTTCAACACACCGCT+TGG | - | 50590:25270-25289 | MS.gene072595:CDS | 50.0% | |
| ! | ATTGGTGGTTCCTGAGGCTT+TGG | - | 50590:25847-25866 | MS.gene072595:CDS | 50.0% |
| ! | GAGAAGTTGATCCTGTGACG+GGG | - | 50590:26131-26150 | MS.gene072595:CDS | 50.0% |
| ! | GAGCTGCCATTGTTGTTGAG+CGG | + | 50590:25105-25124 | None:intergenic | 50.0% |
| ! | GGAGAAGTTGATCCTGTGAC+GGG | - | 50590:26130-26149 | MS.gene072595:CDS | 50.0% |
| ! | TGATGTCTGGGATTGAACGG+TGG | + | 50590:24932-24951 | None:intergenic | 50.0% |
| ! | TGCTCAATTGGTGGTTCCTG+AGG | - | 50590:25841-25860 | MS.gene072595:CDS | 50.0% |
| ! | TTGAAGAACGATAGCGGCAG+CGG | + | 50590:25261-25280 | None:intergenic | 50.0% |
| ! | TTGTTGTTGAGCGGAGAGGA+TGG | + | 50590:25096-25115 | None:intergenic | 50.0% |
| !! | AGTTGTTGATGGCGGGTTTG+AGG | + | 50590:25166-25185 | None:intergenic | 50.0% |
| !! | CAGAGAGGAAGGTGTTGAAC+AGG | - | 50590:25580-25599 | MS.gene072595:CDS | 50.0% |
| !! | GCTGTGGAAAGTGCTTGGAT+TGG | - | 50590:25803-25822 | MS.gene072595:CDS | 50.0% |
| ACGAGTGACGTAAGCCTCCA+TGG | + | 50590:25372-25391 | None:intergenic | 55.0% | |
| ATCCTTGCATACCCCGTCAC+AGG | + | 50590:26145-26164 | None:intergenic | 55.0% | |
| GCCGTCGCTCGCATTAACAA+CGG | - | 50590:25047-25066 | MS.gene072595:CDS | 55.0% | |
| GCCGTTGTTAATGCGAGCGA+CGG | + | 50590:25051-25070 | None:intergenic | 55.0% | |
| GTTGTTAATGCGAGCGACGG+CGG | + | 50590:25048-25067 | None:intergenic | 55.0% | |
| TTGTTGAGCGGAGAGGATGG+AGG | + | 50590:25093-25112 | None:intergenic | 55.0% | |
| ! | ATCCTGTGACGGGGTATGCA+AGG | - | 50590:26140-26159 | MS.gene072595:CDS | 55.0% |
| ! | CTTACGTCACTCGTGCTGGT+GGG | - | 50590:25378-25397 | MS.gene072595:CDS | 55.0% |
| ! | GCCATTGTTGTTGAGCGGAG+AGG | + | 50590:25100-25119 | None:intergenic | 55.0% |
| ! | GGTGTGTGCCGTTGATGTCT+GGG | + | 50590:24944-24963 | None:intergenic | 55.0% |
| ! | TGAGCGGGAGTTGTTGATGG+CGG | + | 50590:25174-25193 | None:intergenic | 55.0% |
| !! | ACCGCTTGGTGTTTCCGTGA+AGG | - | 50590:25284-25303 | MS.gene072595:CDS | 55.0% |
| !! | TGTTGATGGCGGGTTTGAGG+TGG | + | 50590:25163-25182 | None:intergenic | 55.0% |
| GAGGCTTACGTCACTCGTGC+TGG | - | 50590:25374-25393 | MS.gene072595:CDS | 60.0% | |
| GCTTACGTCACTCGTGCTGG+TGG | - | 50590:25377-25396 | MS.gene072595:CDS | 60.0% | |
| GGAGAGGATGGAGGAAGGAC+AGG | + | 50590:25084-25103 | None:intergenic | 60.0% | |
| GGCGTCGTGGTGGCTGAATT+CGG | + | 50590:25027-25046 | None:intergenic | 60.0% | |
| TGAGCGGAGAGGATGGAGGA+AGG | + | 50590:25089-25108 | None:intergenic | 60.0% | |
| ! | AAGAACGATAGCGGCAGCGG+TGG | + | 50590:25258-25277 | None:intergenic | 60.0% |
| ! | GGGTGTGTGCCGTTGATGTC+TGG | + | 50590:24945-24964 | None:intergenic | 60.0% |
| !! | GAGCGGGAGTTGTTGATGGC+GGG | + | 50590:25173-25192 | None:intergenic | 60.0% |
| ! | AACGATAGCGGCAGCGGTGG+TGG | + | 50590:25255-25274 | None:intergenic | 65.0% |
| ! | GAGCGACGGCGGCGTCGTGG+TGG | + | 50590:25037-25056 | None:intergenic | 80.0% |
| ! | TGCGAGCGACGGCGGCGTCG+TGG | + | 50590:25040-25059 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 50590 | gene | 24906 | 26267 | 24906 | ID=MS.gene072595 |
| 50590 | mRNA | 24906 | 26267 | 24906 | ID=MS.gene072595.t1;Parent=MS.gene072595 |
| 50590 | exon | 24906 | 26267 | 24906 | ID=MS.gene072595.t1.exon1;Parent=MS.gene072595.t1 |
| 50590 | CDS | 24906 | 26267 | 24906 | ID=cds.MS.gene072595.t1;Parent=MS.gene072595.t1 |
| Gene Sequence |
| Protein sequence |
