AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07363

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07363.t1 MTR_1g086070 96.659 449 15 0 3 451 2 450 0.0 903
MS.gene07363.t1 MTR_7g111540 60.179 447 165 4 10 450 16 455 0.0 563
MS.gene07363.t1 MTR_1g484920 58.036 448 176 5 5 448 4 443 0.0 526
MS.gene07363.t1 MTR_1g484920 51.794 446 164 5 5 448 4 400 1.83e-154 444
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07363.t1 AT3G62130 64.889 450 148 2 1 449 12 452 0.0 635
MS.gene07363.t1 AT3G62130 64.889 450 148 2 1 449 12 452 0.0 635
MS.gene07363.t1 AT5G26600 60.179 447 164 4 10 448 33 473 0.0 530
MS.gene07363.t1 AT5G26600 60.179 447 164 4 10 448 33 473 0.0 530

Find 134 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATGGTATGGTTGTGAAAA+TGG 0.157149 1.2:+64773933 MS.gene07363:CDS
ATGTTAGTAATTTGTATAAA+TGG 0.173706 1.2:+64773692 MS.gene07363:CDS
GCTTGTGGTGCCGTCGATTA+TGG 0.241155 1.2:+64773861 MS.gene07363:CDS
TGAGGTTGAATTACCGTTTC+CGG 0.253269 1.2:+64773429 MS.gene07363:CDS
GACTGCAATTATTCAGCTTT+TGG 0.256976 1.2:+64774242 MS.gene07363:CDS
TTCCGGTTAGGGAATTGATT+AGG 0.279390 1.2:+64773572 MS.gene07363:CDS
TGAAGTTCCTGTGTATTATC+AGG 0.286901 1.2:+64774110 MS.gene07363:CDS
AGCGGAGCTGCCAGGTGGTT+TGG 0.290209 1.2:-64773126 None:intergenic
AATGTTGATGTTAAGGAAAT+TGG 0.294275 1.2:+64773658 MS.gene07363:CDS
ACACTGTTGATGATTATAAT+AGG 0.298624 1.2:+64774214 MS.gene07363:CDS
GCGGAGCTGCCAGGTGGTTT+GGG 0.312446 1.2:-64773125 None:intergenic
GTTGATGGTGCTCATGCTTT+AGG 0.316255 1.2:+64773628 MS.gene07363:CDS
TGATTATTCAGTCACTCTTT+AGG 0.324233 1.2:-64774291 None:intergenic
GATAAGGATGGGTTTATAAC+TGG 0.327185 1.2:+64774162 MS.gene07363:CDS
ATCATACTCGCACACATTTC+AGG 0.329450 1.2:-64774000 None:intergenic
TCCGGTTAGGGAATTGATTA+GGG 0.334111 1.2:+64773573 MS.gene07363:CDS
CGACGTCATCGGCATTGATT+AGG 0.342101 1.2:-64773225 None:intergenic
AAATTATAACACTGTCATTT+CGG 0.342626 1.2:-64773336 None:intergenic
AGATTAACGGTGGGAGAGTT+AGG 0.343995 1.2:+64773506 MS.gene07363:CDS
TGGTTTGCCTTCAAAGATTC+GGG 0.346535 1.2:+64774029 MS.gene07363:CDS
AAGTTTGAATGTTGATGTTA+AGG 0.365457 1.2:+64773651 MS.gene07363:CDS
ATTTCCTCGTTGGAATTAAC+CGG 0.370491 1.2:-64773448 None:intergenic
CAAGCGTGGGGTACAAATCT+TGG 0.376935 1.2:+64773970 MS.gene07363:CDS
ATGATGATGCTTCGAGGTTA+AGG 0.380388 1.2:+64774061 MS.gene07363:CDS
TTCAGTTGTTATTCCGGTTA+GGG 0.382319 1.2:+64773561 MS.gene07363:CDS
TTGGTTTGCCTTCAAAGATT+CGG 0.383079 1.2:+64774028 MS.gene07363:CDS
CTTCAGTTGTTATTCCGGTT+AGG 0.388055 1.2:+64773560 MS.gene07363:CDS
GTGCGACGGCGGTTTGGTGG+TGG 0.388236 1.2:-64773054 None:intergenic
CCGGTGGTGGCGCATGAGTA+TGG 0.390433 1.2:+64773784 MS.gene07363:CDS
GTTTGCCTGCTGAGAGTGCT+TGG 0.391339 1.2:+64773812 MS.gene07363:CDS
GGCTGAACGGTGCCGTTTCG+AGG 0.394109 1.2:-64772968 None:intergenic
CTGCTGAGAGTGCTTGGGTT+GGG 0.396233 1.2:+64773818 MS.gene07363:CDS
GTCATTTCGGCAGAACTTAC+CGG 0.396727 1.2:-64773323 None:intergenic
AAGAAGTTCGTGTGAGGAAA+TGG 0.397728 1.2:-64773020 None:intergenic
ACCCTAATCAATTCCCTAAC+CGG 0.397893 1.2:-64773574 None:intergenic
GATGCCTTCAGTTGTTATTC+CGG 0.407025 1.2:+64773555 MS.gene07363:CDS
TTATGTGAGGATTTCTCATC+AGG 0.407288 1.2:+64774185 MS.gene07363:CDS
CGGTTTGAAGGTGGAATTGA+AGG 0.407550 1.2:+64773895 MS.gene07363:CDS
CGAATCAACAACGGCAGCTT+CGG 0.409299 1.2:+64773076 MS.gene07363:CDS
GGTGGTTTGGGCGGTGAGGA+TGG 0.410822 1.2:-64773113 None:intergenic
CGAAATTTCTTCGACGTCAT+CGG 0.417067 1.2:-64773236 None:intergenic
GAAATGGAGTGAGTGAGTTT+TGG 0.421190 1.2:-64773004 None:intergenic
AACGATGGCAGCAGCGGTGG+TGG 0.434975 1.2:-64773272 None:intergenic
CATGGTATGGTTGTGAAAAT+GGG 0.436733 1.2:+64773934 MS.gene07363:CDS
CTCATCACCCGAATCTTTGA+AGG 0.440821 1.2:-64774036 None:intergenic
TTTGCCTGCTGAGAGTGCTT+GGG 0.445854 1.2:+64773813 MS.gene07363:CDS
CCTGCTGAGAGTGCTTGGGT+TGG 0.453877 1.2:+64773817 MS.gene07363:CDS
TGATTCGTGCGACGGCGGTT+TGG 0.456057 1.2:-64773060 None:intergenic
TTTGGGCGGTGAGGATGGAG+CGG 0.462678 1.2:-64773108 None:intergenic
TTGGGCGGTGAGGATGGAGC+GGG 0.468335 1.2:-64773107 None:intergenic
GCCATCGTTCTTCAACAAAT+CGG 0.471082 1.2:+64773286 MS.gene07363:CDS
TGCGACGGCGGTTTGGTGGT+GGG 0.473641 1.2:-64773053 None:intergenic
ATTGAAGGAGGGAAGATTAA+CGG 0.474227 1.2:+64773493 MS.gene07363:CDS
ATTATCGCGGAGTTTAAGAA+AGG 0.474569 1.2:+64773469 MS.gene07363:CDS
ATTGATTAGGGTTTGTAGAG+AGG 0.478025 1.2:+64773585 MS.gene07363:CDS
TGTGCGAGTATGATTATGAT+TGG 0.478342 1.2:+64774009 MS.gene07363:CDS
TATTATCAGGCTTTGAGAAA+TGG 0.484635 1.2:+64774123 MS.gene07363:CDS
GCCGTTGTTGATTCGTGCGA+CGG 0.484870 1.2:-64773068 None:intergenic
AAGCGTGGGGTACAAATCTT+GGG 0.486188 1.2:+64773971 MS.gene07363:CDS
GGTTTGTAGAGAGGAAGAAG+TGG 0.491671 1.2:+64773594 MS.gene07363:CDS
TGGGCGGTGAGGATGGAGCG+GGG 0.491672 1.2:-64773106 None:intergenic
TGTCGGTGGCTCTGTTATTG+AGG 0.492187 1.2:+64773411 MS.gene07363:CDS
GGCTTTGAGAAATGGTGAAA+GGG 0.495516 1.2:+64774131 MS.gene07363:CDS
TTTAAGAAAGGGATTGAAGG+AGG 0.501661 1.2:+64773481 MS.gene07363:CDS
CTCCGCGATAATTTCCTCGT+TGG 0.502476 1.2:-64773458 None:intergenic
ATTGAATGATGTTCATCATC+CGG 0.504150 1.2:+64773765 MS.gene07363:CDS
CGATTATGGAGTTTGTGAAT+CGG 0.507392 1.2:+64773875 MS.gene07363:CDS
TCGTTGGAATTAACCGGAAA+CGG 0.508709 1.2:-64773442 None:intergenic
GAGTTTGTGAATCGGTTTGA+AGG 0.509546 1.2:+64773883 MS.gene07363:CDS
TCCTCACCGCCCAAACCACC+TGG 0.513224 1.2:+64773116 MS.gene07363:CDS
GCTGAACGGTGCCGTTTCGA+GGG 0.516516 1.2:-64772967 None:intergenic
TTGAAGAAAGCGGAGCTGCC+AGG 0.524309 1.2:-64773134 None:intergenic
TACATAAACGCAACCGAAGG+TGG 0.527019 1.2:-64773724 None:intergenic
TTAAGAAAGGGATTGAAGGA+GGG 0.529552 1.2:+64773482 MS.gene07363:CDS
CCATACTCATGCGCCACCAC+CGG 0.531694 1.2:-64773784 None:intergenic
TTCGTGCGACGGCGGTTTGG+TGG 0.534624 1.2:-64773057 None:intergenic
TTTGTGAATCGGTTTGAAGG+TGG 0.538570 1.2:+64773886 MS.gene07363:CDS
TGAAAGGGATGCTAGAGATA+AGG 0.538653 1.2:+64774146 MS.gene07363:CDS
CCAACCCAAGCACTCTCAGC+AGG 0.539864 1.2:-64773817 None:intergenic
AACCACGACGACCCTCGAAA+CGG 0.539957 1.2:+64772956 MS.gene07363:CDS
GGTTATGTTGAAGCAAGCGT+GGG 0.542634 1.2:+64773957 MS.gene07363:CDS
AGGCTTTGAGAAATGGTGAA+AGG 0.551471 1.2:+64774130 MS.gene07363:CDS
GAGTTTAAGAAAGGGATTGA+AGG 0.553891 1.2:+64773478 MS.gene07363:CDS
TTTCTTTGAGTCTGGCTGAA+CGG 0.554785 1.2:-64772981 None:intergenic
AGGGATGCTAGAGATAAGGA+TGG 0.555067 1.2:+64774150 MS.gene07363:CDS
GAGAATCGAGGATTCCGTTA+CGG 0.559303 1.2:-64773186 None:intergenic
TTATCGCGGAGTTTAAGAAA+GGG 0.562582 1.2:+64773470 MS.gene07363:CDS
TTCTTCAACACCCTCCGTAA+CGG 0.565314 1.2:+64773172 MS.gene07363:CDS
AAGAACGATGGCAGCAGCGG+TGG 0.568006 1.2:-64773275 None:intergenic
GGGTTATGTTGAAGCAAGCG+TGG 0.571041 1.2:+64773956 MS.gene07363:CDS
GTGTTGAAGAAGAAGTCGTC+GGG 0.572806 1.2:-64773160 None:intergenic
GGTTTATAACTGGTTATGTG+AGG 0.576418 1.2:+64774172 MS.gene07363:CDS
TGATGTTCATCATCCGGTGG+TGG 0.581615 1.2:+64773771 MS.gene07363:CDS
TATGAAGAGGAACCATGGTA+TGG 0.587034 1.2:+64773921 MS.gene07363:CDS
AGATTATAGTCCTCAGCTTG+TGG 0.589039 1.2:+64773846 MS.gene07363:CDS
GGGATGCTAGAGATAAGGAT+GGG 0.591264 1.2:+64774151 MS.gene07363:CDS
AAGGAGGGAAGATTAACGGT+GGG 0.591432 1.2:+64773497 MS.gene07363:CDS
GGTTTGAAGGTGGAATTGAA+GGG 0.591604 1.2:+64773896 MS.gene07363:CDS
TCAATAACAGAGCCACCGAC+AGG 0.591984 1.2:-64773409 None:intergenic
GCCGATTTGTTGAAGAACGA+TGG 0.592414 1.2:-64773287 None:intergenic
GCCAGGTGGTTTGGGCGGTG+AGG 0.592834 1.2:-64773117 None:intergenic
GAGCTGCCAGGTGGTTTGGG+CGG 0.593495 1.2:-64773122 None:intergenic
GTGGCGCATGAGTATGGTAA+TGG 0.597378 1.2:+64773790 MS.gene07363:CDS
GAAGCTTATGTTATACCTGT+CGG 0.603444 1.2:+64773394 MS.gene07363:CDS
GAAGGAGGGAAGATTAACGG+TGG 0.604748 1.2:+64773496 MS.gene07363:CDS
TTGAAGAACGATGGCAGCAG+CGG 0.609909 1.2:-64773278 None:intergenic
ATGGTATGGTTGTGAAAATG+GGG 0.613792 1.2:+64773935 MS.gene07363:CDS
ATCGACGGCACCACAAGCTG+AGG 0.616159 1.2:-64773856 None:intergenic
CTCAAAGCCTGATAATACAC+AGG 0.617796 1.2:-64774117 None:intergenic
TAACAGCTTGATAAGCACAA+TGG 0.618335 1.2:-64773360 None:intergenic
GTTGTTGATTCGTGCGACGG+CGG 0.618562 1.2:-64773065 None:intergenic
TGAGTGATGATGATGCTTCG+AGG 0.619097 1.2:+64774055 MS.gene07363:CDS
GGTGTTGAAGAAGAAGTCGT+CGG 0.621265 1.2:-64773161 None:intergenic
CTAACCGGAATAACAACTGA+AGG 0.626000 1.2:-64773559 None:intergenic
ATCGAGGATTCCGTTACGGA+GGG 0.629759 1.2:-64773182 None:intergenic
ACTTACCGGAGGAAAATTGA+CGG 0.631633 1.2:-64773309 None:intergenic
TTCACAAACTCCATAATCGA+CGG 0.638906 1.2:-64773871 None:intergenic
GAATTGAAGGGATTATGAAG+AGG 0.640801 1.2:+64773908 MS.gene07363:CDS
TTCGTGAAGAAGTTCGTGTG+AGG 0.642301 1.2:-64773026 None:intergenic
CGTCGGGTTGTTGAAGAAAG+CGG 0.644114 1.2:-64773144 None:intergenic
TGCCGTTTCGAGGGTCGTCG+TGG 0.649029 1.2:-64772958 None:intergenic
GGGATTATGAAGAGGAACCA+TGG 0.650885 1.2:+64773916 MS.gene07363:CDS
CAATACATAAACGCAACCGA+AGG 0.652956 1.2:-64773727 None:intergenic
AATCGAGGATTCCGTTACGG+AGG 0.662241 1.2:-64773183 None:intergenic
TTCCAACGAGGAAATTATCG+CGG 0.665860 1.2:+64773456 MS.gene07363:CDS
GCTTATGTTATACCTGTCGG+TGG 0.674775 1.2:+64773397 MS.gene07363:CDS
GTTATGTTGAAGCAAGCGTG+GGG 0.691208 1.2:+64773958 MS.gene07363:CDS
AAGAAAGCGGAGCTGCCAGG+TGG 0.699750 1.2:-64773131 None:intergenic
ATTTCGGCAGAACTTACCGG+AGG 0.711142 1.2:-64773320 None:intergenic
GAATGATGTTCATCATCCGG+TGG 0.719802 1.2:+64773768 MS.gene07363:CDS
GCCGTCGCACGAATCAACAA+CGG 0.725169 1.2:+64773067 MS.gene07363:CDS
ATACTCGCACACATTTCAGG+CGG 0.737674 1.2:-64773997 None:intergenic
TGGTATGGTTGTGAAAATGG+GGG 0.742084 1.2:+64773936 MS.gene07363:CDS
GTTTCCGGTTAATTCCAACG+AGG 0.797229 1.2:+64773444 MS.gene07363:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTTAGTAATTTGTATAAA+TGG + chr1.2:64773692-64773711 MS.gene07363:CDS 15.0%
!!! AAATTATAACACTGTCATTT+CGG - chr1.2:64773339-64773358 None:intergenic 20.0%
! AAGTTTGAATGTTGATGTTA+AGG + chr1.2:64773651-64773670 MS.gene07363:CDS 25.0%
! AATGTTGATGTTAAGGAAAT+TGG + chr1.2:64773658-64773677 MS.gene07363:CDS 25.0%
!!! ACACTGTTGATGATTATAAT+AGG + chr1.2:64774214-64774233 MS.gene07363:CDS 25.0%
ATTGAATGATGTTCATCATC+CGG + chr1.2:64773765-64773784 MS.gene07363:CDS 30.0%
!! ATTATTCAGCTTTTGGAAGA+TGG + chr1.2:64774249-64774268 MS.gene07363:CDS 30.0%
!! GAGGTTAAGGTTTTATCTTA+GGG + chr1.2:64774074-64774093 MS.gene07363:CDS 30.0%
!! TATTATCAGGCTTTGAGAAA+TGG + chr1.2:64774123-64774142 MS.gene07363:CDS 30.0%
ATGGTATGGTTGTGAAAATG+GGG + chr1.2:64773935-64773954 MS.gene07363:CDS 35.0%
ATTATCGCGGAGTTTAAGAA+AGG + chr1.2:64773469-64773488 MS.gene07363:CDS 35.0%
ATTGAAGGAGGGAAGATTAA+CGG + chr1.2:64773493-64773512 MS.gene07363:CDS 35.0%
ATTGATTAGGGTTTGTAGAG+AGG + chr1.2:64773585-64773604 MS.gene07363:CDS 35.0%
ATTTCCTCGTTGGAATTAAC+CGG - chr1.2:64773451-64773470 None:intergenic 35.0%
CATGGTATGGTTGTGAAAAT+GGG + chr1.2:64773934-64773953 MS.gene07363:CDS 35.0%
CGATTATGGAGTTTGTGAAT+CGG + chr1.2:64773875-64773894 MS.gene07363:CDS 35.0%
GAAGCTTATGTTATACCTGT+CGG + chr1.2:64773394-64773413 MS.gene07363:CDS 35.0%
GAATTGAAGGGATTATGAAG+AGG + chr1.2:64773908-64773927 MS.gene07363:CDS 35.0%
GAGTTTAAGAAAGGGATTGA+AGG + chr1.2:64773478-64773497 MS.gene07363:CDS 35.0%
GGTTTATAACTGGTTATGTG+AGG + chr1.2:64774172-64774191 MS.gene07363:CDS 35.0%
TAACAGCTTGATAAGCACAA+TGG - chr1.2:64773363-64773382 None:intergenic 35.0%
TGAAGTTCCTGTGTATTATC+AGG + chr1.2:64774110-64774129 MS.gene07363:CDS 35.0%
TGTGCGAGTATGATTATGAT+TGG + chr1.2:64774009-64774028 MS.gene07363:CDS 35.0%
TTAAGAAAGGGATTGAAGGA+GGG + chr1.2:64773482-64773501 MS.gene07363:CDS 35.0%
TTATCGCGGAGTTTAAGAAA+GGG + chr1.2:64773470-64773489 MS.gene07363:CDS 35.0%
TTATGTGAGGATTTCTCATC+AGG + chr1.2:64774185-64774204 MS.gene07363:CDS 35.0%
TTCACAAACTCCATAATCGA+CGG - chr1.2:64773874-64773893 None:intergenic 35.0%
TTTAAGAAAGGGATTGAAGG+AGG + chr1.2:64773481-64773500 MS.gene07363:CDS 35.0%
! GACTGCAATTATTCAGCTTT+TGG + chr1.2:64774242-64774261 MS.gene07363:CDS 35.0%
! GATAAGGATGGGTTTATAAC+TGG + chr1.2:64774162-64774181 MS.gene07363:CDS 35.0%
! GTGGATCAGATTTTTGTTGA+TGG + chr1.2:64773613-64773632 MS.gene07363:CDS 35.0%
! TTCAGTTGTTATTCCGGTTA+GGG + chr1.2:64773561-64773580 MS.gene07363:CDS 35.0%
!! CGAGGTTAAGGTTTTATCTT+AGG + chr1.2:64774073-64774092 MS.gene07363:CDS 35.0%
!! TTGGTTTGCCTTCAAAGATT+CGG + chr1.2:64774028-64774047 MS.gene07363:CDS 35.0%
!!! AGTTTTGGTTTCTTTGAGTC+TGG - chr1.2:64772992-64773011 None:intergenic 35.0%
AAGAAGTTCGTGTGAGGAAA+TGG - chr1.2:64773023-64773042 None:intergenic 40.0%
ACCCTAATCAATTCCCTAAC+CGG - chr1.2:64773577-64773596 None:intergenic 40.0%
ACTTACCGGAGGAAAATTGA+CGG - chr1.2:64773312-64773331 None:intergenic 40.0%
AGATTATAGTCCTCAGCTTG+TGG + chr1.2:64773846-64773865 MS.gene07363:CDS 40.0%
ATCATACTCGCACACATTTC+AGG - chr1.2:64774003-64774022 None:intergenic 40.0%
CAATACATAAACGCAACCGA+AGG - chr1.2:64773730-64773749 None:intergenic 40.0%
CCATGGTATGGTTGTGAAAA+TGG + chr1.2:64773933-64773952 MS.gene07363:CDS 40.0%
CGAAATTTCTTCGACGTCAT+CGG - chr1.2:64773239-64773258 None:intergenic 40.0%
CTAACCGGAATAACAACTGA+AGG - chr1.2:64773562-64773581 None:intergenic 40.0%
CTCAAAGCCTGATAATACAC+AGG - chr1.2:64774120-64774139 None:intergenic 40.0%
GCCATCGTTCTTCAACAAAT+CGG + chr1.2:64773286-64773305 MS.gene07363:CDS 40.0%
GGTTTGAAGGTGGAATTGAA+GGG + chr1.2:64773896-64773915 MS.gene07363:CDS 40.0%
TATGAAGAGGAACCATGGTA+TGG + chr1.2:64773921-64773940 MS.gene07363:CDS 40.0%
TCCGGTTAGGGAATTGATTA+GGG + chr1.2:64773573-64773592 MS.gene07363:CDS 40.0%
TCGTTGGAATTAACCGGAAA+CGG - chr1.2:64773445-64773464 None:intergenic 40.0%
TGAAAGGGATGCTAGAGATA+AGG + chr1.2:64774146-64774165 MS.gene07363:CDS 40.0%
TGAGGTTGAATTACCGTTTC+CGG + chr1.2:64773429-64773448 MS.gene07363:CDS 40.0%
TGGTATGGTTGTGAAAATGG+GGG + chr1.2:64773936-64773955 MS.gene07363:CDS 40.0%
TTCCAACGAGGAAATTATCG+CGG + chr1.2:64773456-64773475 MS.gene07363:CDS 40.0%
TTCCGGTTAGGGAATTGATT+AGG + chr1.2:64773572-64773591 MS.gene07363:CDS 40.0%
TTTCTTTGAGTCTGGCTGAA+CGG - chr1.2:64772984-64773003 None:intergenic 40.0%
! ATGATGATGCTTCGAGGTTA+AGG + chr1.2:64774061-64774080 MS.gene07363:CDS 40.0%
! CCATTTTCACAACCATACCA+TGG - chr1.2:64773936-64773955 None:intergenic 40.0%
! CTTCAGTTGTTATTCCGGTT+AGG + chr1.2:64773560-64773579 MS.gene07363:CDS 40.0%
! GAAATGGAGTGAGTGAGTTT+TGG - chr1.2:64773007-64773026 None:intergenic 40.0%
! GATGCCTTCAGTTGTTATTC+CGG + chr1.2:64773555-64773574 MS.gene07363:CDS 40.0%
! TGATGATTTTGCGAGAATCG+AGG - chr1.2:64773201-64773220 None:intergenic 40.0%
! TGGTTTGCCTTCAAAGATTC+GGG + chr1.2:64774029-64774048 MS.gene07363:CDS 40.0%
!! AGGCTTTGAGAAATGGTGAA+AGG + chr1.2:64774130-64774149 MS.gene07363:CDS 40.0%
!! GAGTTTGTGAATCGGTTTGA+AGG + chr1.2:64773883-64773902 MS.gene07363:CDS 40.0%
!! GGCTTTGAGAAATGGTGAAA+GGG + chr1.2:64774131-64774150 MS.gene07363:CDS 40.0%
!! TTTGTGAATCGGTTTGAAGG+TGG + chr1.2:64773886-64773905 MS.gene07363:CDS 40.0%
!!! ATGGTTTTTTTCGCCACCTT+CGG + chr1.2:64773711-64773730 MS.gene07363:CDS 40.0%
AAGCGTGGGGTACAAATCTT+GGG + chr1.2:64773971-64773990 MS.gene07363:CDS 45.0%
AAGGAGGGAAGATTAACGGT+GGG + chr1.2:64773497-64773516 MS.gene07363:CDS 45.0%
AGATTAACGGTGGGAGAGTT+AGG + chr1.2:64773506-64773525 MS.gene07363:CDS 45.0%
AGGGATGCTAGAGATAAGGA+TGG + chr1.2:64774150-64774169 MS.gene07363:CDS 45.0%
ATACTCGCACACATTTCAGG+CGG - chr1.2:64774000-64774019 None:intergenic 45.0%
CGGTTTGAAGGTGGAATTGA+AGG + chr1.2:64773895-64773914 MS.gene07363:CDS 45.0%
CTCATCACCCGAATCTTTGA+AGG - chr1.2:64774039-64774058 None:intergenic 45.0%
GAATGATGTTCATCATCCGG+TGG + chr1.2:64773768-64773787 MS.gene07363:CDS 45.0%
GAGAATCGAGGATTCCGTTA+CGG - chr1.2:64773189-64773208 None:intergenic 45.0%
GCTTATGTTATACCTGTCGG+TGG + chr1.2:64773397-64773416 MS.gene07363:CDS 45.0%
GGGATGCTAGAGATAAGGAT+GGG + chr1.2:64774151-64774170 MS.gene07363:CDS 45.0%
GGGATTATGAAGAGGAACCA+TGG + chr1.2:64773916-64773935 MS.gene07363:CDS 45.0%
GGTTATGTTGAAGCAAGCGT+GGG + chr1.2:64773957-64773976 MS.gene07363:CDS 45.0%
GGTTTGTAGAGAGGAAGAAG+TGG + chr1.2:64773594-64773613 MS.gene07363:CDS 45.0%
GTCATTTCGGCAGAACTTAC+CGG - chr1.2:64773326-64773345 None:intergenic 45.0%
GTGTTGAAGAAGAAGTCGTC+GGG - chr1.2:64773163-64773182 None:intergenic 45.0%
GTTATGTTGAAGCAAGCGTG+GGG + chr1.2:64773958-64773977 MS.gene07363:CDS 45.0%
GTTTCCGGTTAATTCCAACG+AGG + chr1.2:64773444-64773463 MS.gene07363:CDS 45.0%
TACATAAACGCAACCGAAGG+TGG - chr1.2:64773727-64773746 None:intergenic 45.0%
TTCGTGAAGAAGTTCGTGTG+AGG - chr1.2:64773029-64773048 None:intergenic 45.0%
TTCTTCAACACCCTCCGTAA+CGG + chr1.2:64773172-64773191 MS.gene07363:CDS 45.0%
! GGTGTTGAAGAAGAAGTCGT+CGG - chr1.2:64773164-64773183 None:intergenic 45.0%
! TGAGTGATGATGATGCTTCG+AGG + chr1.2:64774055-64774074 MS.gene07363:CDS 45.0%
!! GCCGATTTGTTGAAGAACGA+TGG - chr1.2:64773290-64773309 None:intergenic 45.0%
!! GTTGATGGTGCTCATGCTTT+AGG + chr1.2:64773628-64773647 MS.gene07363:CDS 45.0%
AATCGAGGATTCCGTTACGG+AGG - chr1.2:64773186-64773205 None:intergenic 50.0%
ATCGAGGATTCCGTTACGGA+GGG - chr1.2:64773185-64773204 None:intergenic 50.0%
ATTTCGGCAGAACTTACCGG+AGG - chr1.2:64773323-64773342 None:intergenic 50.0%
CAAGCGTGGGGTACAAATCT+TGG + chr1.2:64773970-64773989 MS.gene07363:CDS 50.0%
CGAATCAACAACGGCAGCTT+CGG + chr1.2:64773076-64773095 MS.gene07363:CDS 50.0%
CTCCGCGATAATTTCCTCGT+TGG - chr1.2:64773461-64773480 None:intergenic 50.0%
GAAGGAGGGAAGATTAACGG+TGG + chr1.2:64773496-64773515 MS.gene07363:CDS 50.0%
GGGTTATGTTGAAGCAAGCG+TGG + chr1.2:64773956-64773975 MS.gene07363:CDS 50.0%
GTGGCGCATGAGTATGGTAA+TGG + chr1.2:64773790-64773809 MS.gene07363:CDS 50.0%
TCAATAACAGAGCCACCGAC+AGG - chr1.2:64773412-64773431 None:intergenic 50.0%
TGATGTTCATCATCCGGTGG+TGG + chr1.2:64773771-64773790 MS.gene07363:CDS 50.0%
TTGAAGAACGATGGCAGCAG+CGG - chr1.2:64773281-64773300 None:intergenic 50.0%
! ATCGGCCGTCAATTTTCCTC+CGG + chr1.2:64773304-64773323 MS.gene07363:CDS 50.0%
! CGTCGGGTTGTTGAAGAAAG+CGG - chr1.2:64773147-64773166 None:intergenic 50.0%
! TGTCGGTGGCTCTGTTATTG+AGG + chr1.2:64773411-64773430 MS.gene07363:CDS 50.0%
!! CGACGTCATCGGCATTGATT+AGG - chr1.2:64773228-64773247 None:intergenic 50.0%
!! TTTGCCTGCTGAGAGTGCTT+GGG + chr1.2:64773813-64773832 MS.gene07363:CDS 50.0%
AACCACGACGACCCTCGAAA+CGG + chr1.2:64772956-64772975 MS.gene07363:CDS 55.0%
GCCGTCGCACGAATCAACAA+CGG + chr1.2:64773067-64773086 MS.gene07363:CDS 55.0%
TTGAAGAAAGCGGAGCTGCC+AGG - chr1.2:64773137-64773156 None:intergenic 55.0%
!! CTGCTGAGAGTGCTTGGGTT+GGG + chr1.2:64773818-64773837 MS.gene07363:CDS 55.0%
!! GCCGTTGTTGATTCGTGCGA+CGG - chr1.2:64773071-64773090 None:intergenic 55.0%
!! GCTTGTGGTGCCGTCGATTA+TGG + chr1.2:64773861-64773880 MS.gene07363:CDS 55.0%
!! GTTGTTGATTCGTGCGACGG+CGG - chr1.2:64773068-64773087 None:intergenic 55.0%
!! GTTTGCCTGCTGAGAGTGCT+TGG + chr1.2:64773812-64773831 MS.gene07363:CDS 55.0%
AAGAAAGCGGAGCTGCCAGG+TGG - chr1.2:64773134-64773153 None:intergenic 60.0%
AAGAACGATGGCAGCAGCGG+TGG - chr1.2:64773278-64773297 None:intergenic 60.0%
ATCGACGGCACCACAAGCTG+AGG - chr1.2:64773859-64773878 None:intergenic 60.0%
CCAACCCAAGCACTCTCAGC+AGG - chr1.2:64773820-64773839 None:intergenic 60.0%
CCATACTCATGCGCCACCAC+CGG - chr1.2:64773787-64773806 None:intergenic 60.0%
TGATTCGTGCGACGGCGGTT+TGG - chr1.2:64773063-64773082 None:intergenic 60.0%
TTTGGGCGGTGAGGATGGAG+CGG - chr1.2:64773111-64773130 None:intergenic 60.0%
!! CCTGCTGAGAGTGCTTGGGT+TGG + chr1.2:64773817-64773836 MS.gene07363:CDS 60.0%
!! GCTGAACGGTGCCGTTTCGA+GGG - chr1.2:64772970-64772989 None:intergenic 60.0%
AACGATGGCAGCAGCGGTGG+TGG - chr1.2:64773275-64773294 None:intergenic 65.0%
AGCGGAGCTGCCAGGTGGTT+TGG - chr1.2:64773129-64773148 None:intergenic 65.0%
GCGGAGCTGCCAGGTGGTTT+GGG - chr1.2:64773128-64773147 None:intergenic 65.0%
TCCTCACCGCCCAAACCACC+TGG + chr1.2:64773116-64773135 MS.gene07363:CDS 65.0%
TTGGGCGGTGAGGATGGAGC+GGG - chr1.2:64773110-64773129 None:intergenic 65.0%
! CCGGTGGTGGCGCATGAGTA+TGG + chr1.2:64773784-64773803 MS.gene07363:CDS 65.0%
! GAGCTGCCAGGTGGTTTGGG+CGG - chr1.2:64773125-64773144 None:intergenic 65.0%
! GGTGGTTTGGGCGGTGAGGA+TGG - chr1.2:64773116-64773135 None:intergenic 65.0%
! TGCCGTTTCGAGGGTCGTCG+TGG - chr1.2:64772961-64772980 None:intergenic 65.0%
! TTCGTGCGACGGCGGTTTGG+TGG - chr1.2:64773060-64773079 None:intergenic 65.0%
!! GGCTGAACGGTGCCGTTTCG+AGG - chr1.2:64772971-64772990 None:intergenic 65.0%
!! TGCGACGGCGGTTTGGTGGT+GGG - chr1.2:64773056-64773075 None:intergenic 65.0%
TGGGCGGTGAGGATGGAGCG+GGG - chr1.2:64773109-64773128 None:intergenic 70.0%
! GCCAGGTGGTTTGGGCGGTG+AGG - chr1.2:64773120-64773139 None:intergenic 70.0%
!! GTGCGACGGCGGTTTGGTGG+TGG - chr1.2:64773057-64773076 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 64772947 64774302 64772947 ID=MS.gene07363
chr1.2 mRNA 64772947 64774302 64772947 ID=MS.gene07363.t1;Parent=MS.gene07363
chr1.2 exon 64772947 64774302 64772947 ID=MS.gene07363.t1.exon1;Parent=MS.gene07363.t1
chr1.2 CDS 64772947 64774302 64772947 ID=cds.MS.gene07363.t1;Parent=MS.gene07363.t1
Gene Sequence

>MS.gene07363

ATGGATGATAACCACGACGACCCTCGAAACGGCACCGTTCAGCCAGACTCAAAGAAACCAAAACTCACTCACTCCATTTCCTCACACGAACTTCTTCACGAATTCTCCCACCACCAAACCGCCGTCGCACGAATCAACAACGGCAGCTTCGGCAGCTGTCCCCGCTCCATCCTCACCGCCCAAACCACCTGGCAGCTCCGCTTTCTTCAACAACCCGACGACTTCTTCTTCAACACCCTCCGTAACGGAATCCTCGATTCTCGCAAAATCATCAAGAACCTAATCAATGCCGATGACGTCGAAGAAATTTCGTTAATCGATAATGCCACCACCGCTGCTGCCATCGTTCTTCAACAAATCGGCCGTCAATTTTCCTCCGGTAAGTTCTGCCGAAATGACAGTGTTATAATTTTCCATTGTGCTTATCAAGCTGTTAAGAAATCAATTGAAGCTTATGTTATACCTGTCGGTGGCTCTGTTATTGAGGTTGAATTACCGTTTCCGGTTAATTCCAACGAGGAAATTATCGCGGAGTTTAAGAAAGGGATTGAAGGAGGGAAGATTAACGGTGGGAGAGTTAGGTTAGCGATAATTGATCACATTACGTCGATGCCTTCAGTTGTTATTCCGGTTAGGGAATTGATTAGGGTTTGTAGAGAGGAAGAAGTGGATCAGATTTTTGTTGATGGTGCTCATGCTTTAGGAAGTTTGAATGTTGATGTTAAGGAAATTGGTGCTGATTTTTATGTTAGTAATTTGTATAAATGGTTTTTTTCGCCACCTTCGGTTGCGTTTATGTATTGTAATAAGAATAAGAAATTGAATGATGTTCATCATCCGGTGGTGGCGCATGAGTATGGTAATGGTTTGCCTGCTGAGAGTGCTTGGGTTGGGATGAGAGATTATAGTCCTCAGCTTGTGGTGCCGTCGATTATGGAGTTTGTGAATCGGTTTGAAGGTGGAATTGAAGGGATTATGAAGAGGAACCATGGTATGGTTGTGAAAATGGGGGTTATGTTGAAGCAAGCGTGGGGTACAAATCTTGGGTCGCCGCCTGAAATGTGTGCGAGTATGATTATGATTGGTTTGCCTTCAAAGATTCGGGTGATGAGTGATGATGATGCTTCGAGGTTAAGGTTTTATCTTAGGGTTTATCATGCTATTGAAGTTCCTGTGTATTATCAGGCTTTGAGAAATGGTGAAAGGGATGCTAGAGATAAGGATGGGTTTATAACTGGTTATGTGAGGATTTCTCATCAGGTGTATAACACTGTTGATGATTATAATAGGCTTAAGACTGCAATTATTCAGCTTTTGGAAGATGGAAAAATTTGCAGTGAACTTCCTAAAGAGTGA
Protein sequence

>MS.gene07363.t1

MDDNHDDPRNGTVQPDSKKPKLTHSISSHELLHEFSHHQTAVARINNGSFGSCPRSILTAQTTWQLRFLQQPDDFFFNTLRNGILDSRKIIKNLINADDVEEISLIDNATTAAAIVLQQIGRQFSSGKFCRNDSVIIFHCAYQAVKKSIEAYVIPVGGSVIEVELPFPVNSNEEIIAEFKKGIEGGKINGGRVRLAIIDHITSMPSVVIPVRELIRVCREEEVDQIFVDGAHALGSLNVDVKEIGADFYVSNLYKWFFSPPSVAFMYCNKNKKLNDVHHPVVAHEYGNGLPAESAWVGMRDYSPQLVVPSIMEFVNRFEGGIEGIMKRNHGMVVKMGVMLKQAWGTNLGSPPEMCASMIMIGLPSKIRVMSDDDASRLRFYLRVYHAIEVPVYYQALRNGERDARDKDGFITGYVRISHQVYNTVDDYNRLKTAIIQLLEDGKICSELPKE