Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050796.t1 | XP_003626145.1 | 98 | 455 | 7 | 2 | 1 | 453 | 1 | 455 | 3.40E-262 | 913.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050796.t1 | Q3E6S9 | 71.9 | 430 | 121 | 0 | 23 | 452 | 45 | 474 | 3.5e-191 | 669.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050796.t1 | G7KWF1 | 98.0 | 455 | 7 | 2 | 1 | 453 | 1 | 455 | 2.5e-262 | 913.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050794 | MS.gene050796 | 0.992283 | 2.27E-192 | -1.69E-46 |
| MS.gene050796 | MS.gene072595 | 0.810799 | 9.63E-51 | -1.69E-46 |
| MS.gene050796 | MS.gene072597 | 0.811034 | 8.57E-51 | -1.69E-46 |
| MS.gene050796 | MS.gene072599 | 0.809634 | 1.72E-50 | -1.69E-46 |
| MS.gene050796 | MS.gene22776 | 0.804071 | 2.58E-49 | -1.69E-46 |
| MS.gene050796 | MS.gene23100 | 0.996805 | 1.78E-232 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene050796 | MS.gene005429 | PPI |
| MS.gene050796 | MS.gene20906 | PPI |
| MS.gene050796 | MS.gene07363 | PPI |
| MS.gene050796 | MS.gene20907 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050796.t1 | MTR_7g111540 | 98.022 | 455 | 7 | 2 | 1 | 453 | 1 | 455 | 0.0 | 930 |
| MS.gene050796.t1 | MTR_1g484920 | 71.239 | 452 | 115 | 7 | 4 | 452 | 5 | 444 | 0.0 | 670 |
| MS.gene050796.t1 | MTR_1g484920 | 64.000 | 450 | 108 | 7 | 4 | 452 | 5 | 401 | 0.0 | 583 |
| MS.gene050796.t1 | MTR_1g086070 | 60.225 | 445 | 166 | 4 | 16 | 453 | 9 | 449 | 0.0 | 561 |
| MS.gene050796.t1 | MTR_3g083980 | 23.887 | 247 | 158 | 9 | 52 | 286 | 79 | 307 | 4.25e-11 | 64.7 |
| MS.gene050796.t1 | MTR_3g083980 | 23.887 | 247 | 158 | 9 | 52 | 286 | 79 | 307 | 7.17e-11 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050796.t1 | AT5G26600 | 71.860 | 430 | 121 | 0 | 23 | 452 | 45 | 474 | 0.0 | 659 |
| MS.gene050796.t1 | AT5G26600 | 71.860 | 430 | 121 | 0 | 23 | 452 | 45 | 474 | 0.0 | 659 |
| MS.gene050796.t1 | AT3G62130 | 59.465 | 449 | 171 | 3 | 10 | 451 | 7 | 451 | 0.0 | 583 |
| MS.gene050796.t1 | AT3G62130 | 59.465 | 449 | 171 | 3 | 10 | 451 | 7 | 451 | 0.0 | 583 |
Find 0 sgRNAs with CRISPR-Local
Find 141 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATTGGAAGAAGATAGTTT+AGG | + | 50594:3047-3066 | None:intergenic | 25.0% |
| ! | ACTAAAAAGAATCCTAAAAC+TGG | - | 50594:3794-3813 | MS.gene050796:CDS | 25.0% |
| ! | TTCTTATCAGGTTTACAATA+AGG | - | 50594:4231-4250 | MS.gene050796:CDS | 25.0% |
| !! | ATACTAGTAATTTGCATAAG+TGG | - | 50594:3738-3757 | MS.gene050796:CDS | 25.0% |
| !!! | AAGATAGTTTAGGTTTTTTG+GGG | + | 50594:3037-3056 | None:intergenic | 25.0% |
| !!! | AGAAGATAGTTTAGGTTTTT+TGG | + | 50594:3039-3058 | None:intergenic | 25.0% |
| !!! | GAAGATAGTTTAGGTTTTTT+GGG | + | 50594:3038-3057 | None:intergenic | 25.0% |
| !!! | TTAGAAAAGCTTTGGAAAAA+GGG | - | 50594:3528-3547 | MS.gene050796:CDS | 25.0% |
| !!! | TTTAGAAAAGCTTTGGAAAA+AGG | - | 50594:3527-3546 | MS.gene050796:CDS | 25.0% |
| AAAAAGAATCCTAAAACTGG+TGG | - | 50594:3797-3816 | MS.gene050796:CDS | 30.0% | |
| ATACTAGCACACATATGTTT+CGG | + | 50594:4052-4071 | None:intergenic | 30.0% | |
| CTTTGTTGAATTTACCTTCA+CGG | + | 50594:3367-3386 | None:intergenic | 30.0% | |
| GATGTTGATATGCAAGATAT+TGG | - | 50594:3704-3723 | MS.gene050796:CDS | 30.0% | |
| GTTATGCTTCATTATGCTTA+CGG | - | 50594:3398-3417 | MS.gene050796:CDS | 30.0% | |
| TGGGAAGAAAATTAGATTAG+CGG | - | 50594:3559-3578 | MS.gene050796:CDS | 30.0% | |
| TTGAGGACTATTACAAGTTT+CGG | - | 50594:4254-4273 | MS.gene050796:CDS | 30.0% | |
| ! | GATTTTGTGAATAGGTTTGA+AGG | - | 50594:3935-3954 | MS.gene050796:CDS | 30.0% |
| !!! | AGATAGTTTAGGTTTTTTGG+GGG | + | 50594:3036-3055 | None:intergenic | 30.0% |
| AAGGGATTATAGTGCTCAAT+TGG | - | 50594:3895-3914 | MS.gene050796:CDS | 35.0% | |
| ATCTGAATCAATTCCTTCAC+AGG | + | 50594:3623-3642 | None:intergenic | 35.0% | |
| CATGAAGCTGTTATTGAGAT+GGG | - | 50594:3986-4005 | MS.gene050796:CDS | 35.0% | |
| CGTGAAGGTAAATTCAACAA+AGG | - | 50594:3365-3384 | MS.gene050796:CDS | 35.0% | |
| CTAGGCGGTCTATAATATAT+AGG | + | 50594:4172-4191 | None:intergenic | 35.0% | |
| GAAACATATGTGTGCTAGTA+TGG | - | 50594:4051-4070 | MS.gene050796:CDS | 35.0% | |
| GACTGAGTTTAGAAAAGCTT+TGG | - | 50594:3520-3539 | MS.gene050796:CDS | 35.0% | |
| GTATGCAAGGATTTCTTATC+AGG | - | 50594:4219-4238 | MS.gene050796:CDS | 35.0% | |
| TATGTGTGCTAGTATGGTTA+TGG | - | 50594:4057-4076 | MS.gene050796:CDS | 35.0% | |
| TCAGGTTTACAATAAGGTTG+AGG | - | 50594:4237-4256 | MS.gene050796:CDS | 35.0% | |
| TCATGAAGCTGTTATTGAGA+TGG | - | 50594:3985-4004 | MS.gene050796:CDS | 35.0% | |
| TGAGGACTATTACAAGTTTC+GGG | - | 50594:4255-4274 | MS.gene050796:CDS | 35.0% | |
| TTGGAAAAAGGGAAAGTTGA+TGG | - | 50594:3539-3558 | MS.gene050796:CDS | 35.0% | |
| TTGTGAATAGGTTTGAAGGA+GGG | - | 50594:3939-3958 | MS.gene050796:CDS | 35.0% | |
| TTTGTGAATAGGTTTGAAGG+AGG | - | 50594:3938-3957 | MS.gene050796:CDS | 35.0% | |
| ! | ACACATTTGAGGGATGTTTT+TGG | - | 50594:4136-4155 | MS.gene050796:CDS | 35.0% |
| ! | ATTGATTCAGATTTGCAGAG+AGG | - | 50594:3631-3650 | MS.gene050796:CDS | 35.0% |
| ! | TGGAAAAAGGGAAAGTTGAT+GGG | - | 50594:3540-3559 | MS.gene050796:CDS | 35.0% |
| ! | TTAACCAACTGGTGGATAAT+GGG | - | 50594:4284-4303 | MS.gene050796:CDS | 35.0% |
| ! | TTCGGATTCAATTTCTGAAG+CGG | + | 50594:3075-3094 | None:intergenic | 35.0% |
| !!! | AGGCTTTGGATTTTGTGAAT+AGG | - | 50594:3927-3946 | MS.gene050796:CDS | 35.0% |
| !!! | CTAGGTTTTGGATAATTGAG+CGG | + | 50594:3256-3275 | None:intergenic | 35.0% |
| !!! | TAGGTTTTGGATAATTGAGC+GGG | + | 50594:3255-3274 | None:intergenic | 35.0% |
| !!! | TTGGTTTGCCTACTTGTTTT+GGG | - | 50594:4080-4099 | MS.gene050796:CDS | 35.0% |
| AAGAATCCTAAAACTGGTGG+TGG | - | 50594:3800-3819 | MS.gene050796:CDS | 40.0% | |
| ATTCAGATTTGCAGAGAGGA+AGG | - | 50594:3635-3654 | MS.gene050796:CDS | 40.0% | |
| ATTCTGATGCACTTAAGCTG+AGG | - | 50594:4113-4132 | MS.gene050796:CDS | 40.0% | |
| CTTCTAACCCCAAAACAAGT+AGG | + | 50594:4091-4110 | None:intergenic | 40.0% | |
| GGATTATAGTGCTCAATTGG+TGG | - | 50594:3898-3917 | MS.gene050796:CDS | 40.0% | |
| GGATTGAAGGGATTAAGAAG+AGG | - | 50594:3960-3979 | MS.gene050796:CDS | 40.0% | |
| GTATACAAAAACGCGATCGA+AGG | + | 50594:3776-3795 | None:intergenic | 40.0% | |
| GTGGTTGAAGTAGAAGTGAT+CGG | + | 50594:3210-3229 | None:intergenic | 40.0% | |
| GTGTCTCATGAGTATGGAAA+TGG | - | 50594:3842-3861 | MS.gene050796:CDS | 40.0% | |
| TAAGCTGAGGACACATTTGA+GGG | - | 50594:4126-4145 | MS.gene050796:CDS | 40.0% | |
| TATTATAGACCGCCTAGAGA+TGG | - | 50594:4175-4194 | MS.gene050796:CDS | 40.0% | |
| TATTGAGATGGGAGAGATGT+TGG | - | 50594:3997-4016 | MS.gene050796:CDS | 40.0% | |
| TGCTGTTAAGAAATCCATGG+AGG | - | 50594:3421-3440 | MS.gene050796:CDS | 40.0% | |
| TGGTTGAAGTAGAAGTGATC+GGG | + | 50594:3209-3228 | None:intergenic | 40.0% | |
| TGTGCTAGTATGGTTATGGT+TGG | - | 50594:4061-4080 | MS.gene050796:CDS | 40.0% | |
| TTAAGCTGAGGACACATTTG+AGG | - | 50594:4125-4144 | MS.gene050796:CDS | 40.0% | |
| TTCACAAAATCCAAAGCCTC+AGG | + | 50594:3926-3945 | None:intergenic | 40.0% | |
| TTCACAGGAATCACAACACA+AGG | + | 50594:3608-3627 | None:intergenic | 40.0% | |
| TTGTGTTGTGATTCCTGTGA+AGG | - | 50594:3607-3626 | MS.gene050796:CDS | 40.0% | |
| ! | AAATCACCACCACCAGTTTT+AGG | + | 50594:3809-3828 | None:intergenic | 40.0% |
| ! | AGAGATGTTGGTGAAAGCTT+GGG | - | 50594:4009-4028 | MS.gene050796:CDS | 40.0% |
| ! | GTTAACCAACTGGTGGATAA+TGG | - | 50594:4283-4302 | MS.gene050796:CDS | 40.0% |
| ! | TGAAGTTCCGTTACCTTTTC+CGG | - | 50594:3475-3494 | MS.gene050796:CDS | 40.0% |
| !! | GTATGGAAATGGATTAGCTG+TGG | - | 50594:3853-3872 | MS.gene050796:CDS | 40.0% |
| !! | GTGGTGATTGTTTTTCTCAG+AGG | + | 50594:2979-2998 | None:intergenic | 40.0% |
| !! | TGATGTCTTTAGCGTTGACT+AGG | + | 50594:3274-3293 | None:intergenic | 40.0% |
| !! | TTCTGAAGCGGTGATGAAAT+TGG | + | 50594:3063-3082 | None:intergenic | 40.0% |
| !!! | AGTTTAGGTTTTTTGGGGGT+GGG | + | 50594:3032-3051 | None:intergenic | 40.0% |
| !!! | CTTTAGCGTTGACTAGGTTT+TGG | + | 50594:3268-3287 | None:intergenic | 40.0% |
| !!! | GTTGGTTTGCCTACTTGTTT+TGG | - | 50594:4079-4098 | MS.gene050796:CDS | 40.0% |
| !!! | TAGTTTAGGTTTTTTGGGGG+TGG | + | 50594:3033-3052 | None:intergenic | 40.0% |
| !!! | TGGTTTGCCTACTTGTTTTG+GGG | - | 50594:4081-4100 | MS.gene050796:CDS | 40.0% |
| AAAGCTTGGGGAACTCATCT+TGG | - | 50594:4022-4041 | MS.gene050796:CDS | 45.0% | |
| AAGCTTGGGGAACTCATCTT+GGG | - | 50594:4023-4042 | MS.gene050796:CDS | 45.0% | |
| AATTGAGCGGGAGTTGTTGA+TGG | + | 50594:3243-3262 | None:intergenic | 45.0% | |
| AGGTTTGAAGGAGGGATTGA+AGG | - | 50594:3947-3966 | MS.gene050796:CDS | 45.0% | |
| ATCTCATCGTTCGAACTCAC+CGG | + | 50594:3497-3516 | None:intergenic | 45.0% | |
| CAAACCCATTATCCACCAGT+TGG | + | 50594:4291-4310 | None:intergenic | 45.0% | |
| CCATACTCATGAGACACAAC+AGG | + | 50594:3839-3858 | None:intergenic | 45.0% | |
| CCTGTTGTGTCTCATGAGTA+TGG | - | 50594:3836-3855 | MS.gene050796:CDS | 45.0% | |
| CGTTCAATCCCAGACATCAA+CGG | - | 50594:2999-3018 | MS.gene050796:CDS | 45.0% | |
| GAACTCACCGGAAAAGGTAA+CGG | + | 50594:3485-3504 | None:intergenic | 45.0% | |
| GGATCAACTTCTCCATCTCT+AGG | + | 50594:4190-4209 | None:intergenic | 45.0% | |
| GGTGTGTTGAAGAACGATAG+CGG | + | 50594:3333-3352 | None:intergenic | 45.0% | |
| GGTTTGAAGGAGGGATTGAA+GGG | - | 50594:3948-3967 | MS.gene050796:CDS | 45.0% | |
| GTTGATGCTGCTCATGCAAT+TGG | - | 50594:3674-3693 | MS.gene050796:CDS | 45.0% | |
| TACAAAAACGCGATCGAAGG+TGG | + | 50594:3773-3792 | None:intergenic | 45.0% | |
| TCAACTTCTCCATCTCTAGG+CGG | + | 50594:4187-4206 | None:intergenic | 45.0% | |
| ! | CGTTGATGTCTGGGATTGAA+CGG | + | 50594:3001-3020 | None:intergenic | 45.0% |
| ! | GAGAGATGTTGGTGAAAGCT+TGG | - | 50594:4008-4027 | MS.gene050796:CDS | 45.0% |
| ! | GAGATGTTGGTGAAAGCTTG+GGG | - | 50594:4010-4029 | MS.gene050796:CDS | 45.0% |
| ! | TGGAGAAGTTGATCCTGTGA+CGG | - | 50594:4195-4214 | MS.gene050796:CDS | 45.0% |
| !! | AAAGTGCTTGGATTGGCACA+AGG | - | 50594:3876-3895 | MS.gene050796:CDS | 45.0% |
| !! | AAGTGCTTGGATTGGCACAA+GGG | - | 50594:3877-3896 | MS.gene050796:CDS | 45.0% |
| !! | CGGTGCTGTTAAGAAATCCA+TGG | - | 50594:3418-3437 | MS.gene050796:CDS | 45.0% |
| !! | GATTAGCTGTGGAAAGTGCT+TGG | - | 50594:3864-3883 | MS.gene050796:CDS | 45.0% |
| !!! | GTTTAGGTTTTTTGGGGGTG+GGG | + | 50594:3031-3050 | None:intergenic | 45.0% |
| ACCTTCACGGAAACACCAAG+CGG | + | 50594:3354-3373 | None:intergenic | 50.0% | |
| CGCATTAACAACGGCAGCTT+TGG | - | 50594:3122-3141 | MS.gene050796:CDS | 50.0% | |
| GCCGTTGCTCGCATTAACAA+CGG | - | 50594:3113-3132 | MS.gene050796:CDS | 50.0% | |
| GCCGTTGTTAATGCGAGCAA+CGG | + | 50594:3117-3136 | None:intergenic | 50.0% | |
| GGATGCTGTTAACCAACTGG+TGG | - | 50594:4276-4295 | MS.gene050796:CDS | 50.0% | |
| GTTGTTAATGCGAGCAACGG+CGG | + | 50594:3114-3133 | None:intergenic | 50.0% | |
| TCCTCTCCGCTCAACAACAA+TGG | - | 50594:3162-3181 | MS.gene050796:CDS | 50.0% | |
| TCGGGATGCTGTTAACCAAC+TGG | - | 50594:4273-4292 | MS.gene050796:CDS | 50.0% | |
| TCGTTCGAACTCACCGGAAA+AGG | + | 50594:3491-3510 | None:intergenic | 50.0% | |
| TCGTTCTTCAACACACCGCT+TGG | - | 50594:3336-3355 | MS.gene050796:CDS | 50.0% | |
| ! | ATTGGTGGTTCCTGAGGCTT+TGG | - | 50594:3913-3932 | MS.gene050796:CDS | 50.0% |
| ! | GAGAAGTTGATCCTGTGACG+GGG | - | 50594:4197-4216 | MS.gene050796:CDS | 50.0% |
| ! | GAGCTGCCATTGTTGTTGAG+CGG | + | 50594:3171-3190 | None:intergenic | 50.0% |
| ! | GGAGAAGTTGATCCTGTGAC+GGG | - | 50594:4196-4215 | MS.gene050796:CDS | 50.0% |
| ! | TGATGTCTGGGATTGAACGG+TGG | + | 50594:2998-3017 | None:intergenic | 50.0% |
| ! | TGCTCAATTGGTGGTTCCTG+AGG | - | 50594:3907-3926 | MS.gene050796:CDS | 50.0% |
| ! | TTGAAGAACGATAGCGGCAG+CGG | + | 50594:3327-3346 | None:intergenic | 50.0% |
| ! | TTGTTGTTGAGCGGAGAGGA+TGG | + | 50594:3162-3181 | None:intergenic | 50.0% |
| !! | AGTTGTTGATGGCGGGTTTG+AGG | + | 50594:3232-3251 | None:intergenic | 50.0% |
| !! | CAGAGAGGAAGGTGTTGAAC+AGG | - | 50594:3646-3665 | MS.gene050796:CDS | 50.0% |
| !! | GCTGTGGAAAGTGCTTGGAT+TGG | - | 50594:3869-3888 | MS.gene050796:CDS | 50.0% |
| ACGAGTGACGTAAGCCTCCA+TGG | + | 50594:3438-3457 | None:intergenic | 55.0% | |
| ATCCTTGCATACCCCGTCAC+AGG | + | 50594:4211-4230 | None:intergenic | 55.0% | |
| TTGTTGAGCGGAGAGGATGG+AGG | + | 50594:3159-3178 | None:intergenic | 55.0% | |
| ! | ATCCTGTGACGGGGTATGCA+AGG | - | 50594:4206-4225 | MS.gene050796:CDS | 55.0% |
| ! | CTTACGTCACTCGTGCTGGT+GGG | - | 50594:3444-3463 | MS.gene050796:CDS | 55.0% |
| ! | GCCATTGTTGTTGAGCGGAG+AGG | + | 50594:3166-3185 | None:intergenic | 55.0% |
| ! | GGTGTGTGCCGTTGATGTCT+GGG | + | 50594:3010-3029 | None:intergenic | 55.0% |
| ! | TGAGCGGGAGTTGTTGATGG+CGG | + | 50594:3240-3259 | None:intergenic | 55.0% |
| !! | ACCGCTTGGTGTTTCCGTGA+AGG | - | 50594:3350-3369 | MS.gene050796:CDS | 55.0% |
| !! | TGTTGATGGCGGGTTTGAGG+TGG | + | 50594:3229-3248 | None:intergenic | 55.0% |
| GAGGCTTACGTCACTCGTGC+TGG | - | 50594:3440-3459 | MS.gene050796:CDS | 60.0% | |
| GCTTACGTCACTCGTGCTGG+TGG | - | 50594:3443-3462 | MS.gene050796:CDS | 60.0% | |
| GGAGAGGATGGAGGAAGGAC+AGG | + | 50594:3150-3169 | None:intergenic | 60.0% | |
| GGCGTCGTGGTGGCTGAATT+CGG | + | 50594:3093-3112 | None:intergenic | 60.0% | |
| TGAGCGGAGAGGATGGAGGA+AGG | + | 50594:3155-3174 | None:intergenic | 60.0% | |
| ! | AAGAACGATAGCGGCAGCGG+TGG | + | 50594:3324-3343 | None:intergenic | 60.0% |
| ! | GGGTGTGTGCCGTTGATGTC+TGG | + | 50594:3011-3030 | None:intergenic | 60.0% |
| !! | GAGCGGGAGTTGTTGATGGC+GGG | + | 50594:3239-3258 | None:intergenic | 60.0% |
| ! | AACGATAGCGGCAGCGGTGG+TGG | + | 50594:3321-3340 | None:intergenic | 65.0% |
| GAGCAACGGCGGCGTCGTGG+TGG | + | 50594:3103-3122 | None:intergenic | 75.0% | |
| TGCGAGCAACGGCGGCGTCG+TGG | + | 50594:3106-3125 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 50594 | gene | 2972 | 4333 | 2972 | ID=MS.gene050796 |
| 50594 | mRNA | 2972 | 4333 | 2972 | ID=MS.gene050796.t1;Parent=MS.gene050796 |
| 50594 | exon | 2972 | 4333 | 2972 | ID=MS.gene050796.t1.exon1;Parent=MS.gene050796.t1 |
| 50594 | CDS | 2972 | 4333 | 2972 | ID=cds.MS.gene050796.t1;Parent=MS.gene050796.t1 |
| Gene Sequence |
| Protein sequence |
