AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene005429

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005429.t1 MTR_1g086070 96.659 449 15 0 3 451 2 450 0.0 903
MS.gene005429.t1 MTR_7g111540 60.179 447 165 4 10 450 16 455 0.0 563
MS.gene005429.t1 MTR_1g484920 58.036 448 176 5 5 448 4 443 0.0 526
MS.gene005429.t1 MTR_1g484920 51.794 446 164 5 5 448 4 400 1.83e-154 444
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005429.t1 AT3G62130 64.889 450 148 2 1 449 12 452 0.0 635
MS.gene005429.t1 AT3G62130 64.889 450 148 2 1 449 12 452 0.0 635
MS.gene005429.t1 AT5G26600 60.179 447 164 4 10 448 33 473 0.0 530
MS.gene005429.t1 AT5G26600 60.179 447 164 4 10 448 33 473 0.0 530

Find 133 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATGGTATGGTTGTGAAAA+TGG 0.157149 1.1:+63680598 MS.gene005429:CDS
ATGTTAGTAATTTGTATAAA+TGG 0.173706 1.1:+63680357 MS.gene005429:CDS
GCTTGTGGTGCCGTCGATTA+TGG 0.241155 1.1:+63680526 MS.gene005429:CDS
TGAGGTTGAATTACCGTTTC+CGG 0.253269 1.1:+63680094 MS.gene005429:CDS
GACTGCAATTATTCAGCTTT+TGG 0.256976 1.1:+63680907 MS.gene005429:CDS
TTCCGGTTAGGGAATTGATT+AGG 0.279390 1.1:+63680237 MS.gene005429:CDS
TGAAGTTCCTGTGTATTATC+AGG 0.286901 1.1:+63680775 MS.gene005429:CDS
AGCGGAGCTGCCAGGTGGTT+TGG 0.290209 1.1:-63679791 None:intergenic
AATGTTGATGTTAAGGAAAT+TGG 0.294275 1.1:+63680323 MS.gene005429:CDS
GCGGAGCTGCCAGGTGGTTT+GGG 0.312446 1.1:-63679790 None:intergenic
GTTGATGGTGCTCATGCTTT+AGG 0.316255 1.1:+63680293 MS.gene005429:CDS
TGATTATTCAGTCACTCTTT+AGG 0.324233 1.1:-63680956 None:intergenic
GATAAGGATGGGTTTATAAC+TGG 0.327185 1.1:+63680827 MS.gene005429:CDS
ATCATACTCGCACACATTTC+AGG 0.329450 1.1:-63680665 None:intergenic
TCCGGTTAGGGAATTGATTA+GGG 0.334111 1.1:+63680238 MS.gene005429:CDS
CGACGTCATCGGCATTGATT+AGG 0.342101 1.1:-63679890 None:intergenic
AAATTATAACACTGTCATTT+CGG 0.342626 1.1:-63680001 None:intergenic
AGATTAACGGTGGGAGAGTT+AGG 0.343995 1.1:+63680171 MS.gene005429:CDS
TGGTTTGCCTTCAAAGATTC+GGG 0.346535 1.1:+63680694 MS.gene005429:CDS
AAGTTTGAATGTTGATGTTA+AGG 0.365457 1.1:+63680316 MS.gene005429:CDS
ATTTCCTCGTTGGAATTAAC+CGG 0.370491 1.1:-63680113 None:intergenic
GATGCCTTCAGTTGTTATTC+CGG 0.374083 1.1:+63680220 MS.gene005429:CDS
CAAGCGTGGGGTACAAATCT+TGG 0.376935 1.1:+63680635 MS.gene005429:CDS
ATGATGATGCTTCGAGGTTA+AGG 0.380388 1.1:+63680726 MS.gene005429:CDS
TTCAGTTGTTATTCCGGTTA+GGG 0.382319 1.1:+63680226 MS.gene005429:CDS
TTGGTTTGCCTTCAAAGATT+CGG 0.383079 1.1:+63680693 MS.gene005429:CDS
CTTCAGTTGTTATTCCGGTT+AGG 0.388055 1.1:+63680225 MS.gene005429:CDS
GTGCGACGGCGGTTTGGTGG+TGG 0.388236 1.1:-63679719 None:intergenic
CCGGTGGTGGCGCATGAGTA+TGG 0.390433 1.1:+63680449 MS.gene005429:CDS
GTTTGCCTGCTGAGAGTGCT+TGG 0.391339 1.1:+63680477 MS.gene005429:CDS
GGCTGAACGGTGCCGTTTCG+AGG 0.394109 1.1:-63679633 None:intergenic
CTGCTGAGAGTGCTTGGGTT+GGG 0.396233 1.1:+63680483 MS.gene005429:CDS
GTCATTTCGGCAGAACTTAC+CGG 0.396727 1.1:-63679988 None:intergenic
AAGAAGTTCGTGTGAGGAAA+TGG 0.397728 1.1:-63679685 None:intergenic
ACCCTAATCAATTCCCTAAC+CGG 0.397893 1.1:-63680239 None:intergenic
CGGTTTGAAGGTGGAATTGA+AGG 0.407550 1.1:+63680560 MS.gene005429:CDS
CGAATCAACAACGGCAGCTT+CGG 0.409299 1.1:+63679741 MS.gene005429:CDS
GGTGGTTTGGGCGGTGAGGA+TGG 0.410822 1.1:-63679778 None:intergenic
CGAAATTTCTTCGACGTCAT+CGG 0.417067 1.1:-63679901 None:intergenic
GAAATGGAGTGAGTGAGTTT+TGG 0.421190 1.1:-63679669 None:intergenic
TTACGTGAGGATTTCTCATC+AGG 0.434056 1.1:+63680850 MS.gene005429:CDS
AACGATGGCAGCAGCGGTGG+TGG 0.434975 1.1:-63679937 None:intergenic
CATGGTATGGTTGTGAAAAT+GGG 0.436733 1.1:+63680599 MS.gene005429:CDS
CTCATCACCCGAATCTTTGA+AGG 0.440821 1.1:-63680701 None:intergenic
TTTGCCTGCTGAGAGTGCTT+GGG 0.445854 1.1:+63680478 MS.gene005429:CDS
CCTGCTGAGAGTGCTTGGGT+TGG 0.453877 1.1:+63680482 MS.gene005429:CDS
TGATTCGTGCGACGGCGGTT+TGG 0.456057 1.1:-63679725 None:intergenic
TTTGGGCGGTGAGGATGGAG+CGG 0.462678 1.1:-63679773 None:intergenic
TTGGGCGGTGAGGATGGAGC+GGG 0.468335 1.1:-63679772 None:intergenic
GCCATCGTTCTTCAACAAAT+CGG 0.471082 1.1:+63679951 MS.gene005429:CDS
TGCGACGGCGGTTTGGTGGT+GGG 0.473641 1.1:-63679718 None:intergenic
ATTGAAGGAGGGAAGATTAA+CGG 0.474227 1.1:+63680158 MS.gene005429:CDS
ATTATCGCGGAGTTTAAGAA+AGG 0.474569 1.1:+63680134 MS.gene005429:CDS
ATTGATTAGGGTTTGTAGAG+AGG 0.478025 1.1:+63680250 MS.gene005429:CDS
TGTGCGAGTATGATTATGAT+TGG 0.478342 1.1:+63680674 MS.gene005429:CDS
TATTATCAGGCTTTGAGAAA+TGG 0.484635 1.1:+63680788 MS.gene005429:CDS
GCCGTTGTTGATTCGTGCGA+CGG 0.484870 1.1:-63679733 None:intergenic
AAGCGTGGGGTACAAATCTT+GGG 0.486188 1.1:+63680636 MS.gene005429:CDS
GGTTTGTAGAGAGGAAGAAG+TGG 0.491671 1.1:+63680259 MS.gene005429:CDS
TGGGCGGTGAGGATGGAGCG+GGG 0.491672 1.1:-63679771 None:intergenic
TGTCGGTGGCTCTGTTATTG+AGG 0.492187 1.1:+63680076 MS.gene005429:CDS
GGCTTTGAGAAATGGTGAAA+GGG 0.495516 1.1:+63680796 MS.gene005429:CDS
TTTAAGAAAGGGATTGAAGG+AGG 0.501661 1.1:+63680146 MS.gene005429:CDS
CTCCGCGATAATTTCCTCGT+TGG 0.502476 1.1:-63680123 None:intergenic
ATTGAATGATGTTCATCATC+CGG 0.504150 1.1:+63680430 MS.gene005429:CDS
CGATTATGGAGTTTGTGAAT+CGG 0.507392 1.1:+63680540 MS.gene005429:CDS
TCGTTGGAATTAACCGGAAA+CGG 0.508709 1.1:-63680107 None:intergenic
GAGTTTGTGAATCGGTTTGA+AGG 0.509546 1.1:+63680548 MS.gene005429:CDS
TCCTCACCGCCCAAACCACC+TGG 0.513224 1.1:+63679781 MS.gene005429:CDS
GCTGAACGGTGCCGTTTCGA+GGG 0.516516 1.1:-63679632 None:intergenic
TTGAAGAAAGCGGAGCTGCC+AGG 0.524309 1.1:-63679799 None:intergenic
TACATAAACGCAACCGAAGG+TGG 0.527019 1.1:-63680389 None:intergenic
TTAAGAAAGGGATTGAAGGA+GGG 0.529552 1.1:+63680147 MS.gene005429:CDS
CCATACTCATGCGCCACCAC+CGG 0.531694 1.1:-63680449 None:intergenic
TTCGTGCGACGGCGGTTTGG+TGG 0.534624 1.1:-63679722 None:intergenic
TTTGTGAATCGGTTTGAAGG+TGG 0.538570 1.1:+63680551 MS.gene005429:CDS
TGAAAGGGATGCTAGAGATA+AGG 0.538653 1.1:+63680811 MS.gene005429:CDS
CCAACCCAAGCACTCTCAGC+AGG 0.539864 1.1:-63680482 None:intergenic
AACCACGACGACCCTCGAAA+CGG 0.539957 1.1:+63679621 MS.gene005429:CDS
GGTTATGTTGAAGCAAGCGT+GGG 0.542634 1.1:+63680622 MS.gene005429:CDS
AGGCTTTGAGAAATGGTGAA+AGG 0.551471 1.1:+63680795 MS.gene005429:CDS
GAGTTTAAGAAAGGGATTGA+AGG 0.553891 1.1:+63680143 MS.gene005429:CDS
TTTCTTTGAGTCTGGCTGAA+CGG 0.554785 1.1:-63679646 None:intergenic
AGGGATGCTAGAGATAAGGA+TGG 0.555067 1.1:+63680815 MS.gene005429:CDS
GAGAATCGAGGATTCCGTTA+CGG 0.559303 1.1:-63679851 None:intergenic
TTATCGCGGAGTTTAAGAAA+GGG 0.562582 1.1:+63680135 MS.gene005429:CDS
TTCTTCAACACCCTCCGTAA+CGG 0.565314 1.1:+63679837 MS.gene005429:CDS
AAGAACGATGGCAGCAGCGG+TGG 0.568006 1.1:-63679940 None:intergenic
GGGTTATGTTGAAGCAAGCG+TGG 0.571041 1.1:+63680621 MS.gene005429:CDS
GTGTTGAAGAAGAAGTCGTC+GGG 0.572806 1.1:-63679825 None:intergenic
TGATGTTCATCATCCGGTGG+TGG 0.581615 1.1:+63680436 MS.gene005429:CDS
TATGAAGAGGAACCATGGTA+TGG 0.587034 1.1:+63680586 MS.gene005429:CDS
AGATTATAGTCCTCAGCTTG+TGG 0.589039 1.1:+63680511 MS.gene005429:CDS
GGGATGCTAGAGATAAGGAT+GGG 0.591264 1.1:+63680816 MS.gene005429:CDS
AAGGAGGGAAGATTAACGGT+GGG 0.591432 1.1:+63680162 MS.gene005429:CDS
GGTTTGAAGGTGGAATTGAA+GGG 0.591604 1.1:+63680561 MS.gene005429:CDS
TCAATAACAGAGCCACCGAC+AGG 0.591984 1.1:-63680074 None:intergenic
GCCGATTTGTTGAAGAACGA+TGG 0.592414 1.1:-63679952 None:intergenic
GCCAGGTGGTTTGGGCGGTG+AGG 0.592834 1.1:-63679782 None:intergenic
GAGCTGCCAGGTGGTTTGGG+CGG 0.593495 1.1:-63679787 None:intergenic
GTGGCGCATGAGTATGGTAA+TGG 0.597378 1.1:+63680455 MS.gene005429:CDS
GAAGCTTATGTTATACCTGT+CGG 0.603444 1.1:+63680059 MS.gene005429:CDS
GAAGGAGGGAAGATTAACGG+TGG 0.604748 1.1:+63680161 MS.gene005429:CDS
GGTTTATAACTGGTTACGTG+AGG 0.605700 1.1:+63680837 MS.gene005429:CDS
TTGAAGAACGATGGCAGCAG+CGG 0.609909 1.1:-63679943 None:intergenic
ATGGTATGGTTGTGAAAATG+GGG 0.613792 1.1:+63680600 MS.gene005429:CDS
ATCGACGGCACCACAAGCTG+AGG 0.616159 1.1:-63680521 None:intergenic
CTCAAAGCCTGATAATACAC+AGG 0.617796 1.1:-63680782 None:intergenic
TAACAGCTTGATAAGCACAA+TGG 0.618335 1.1:-63680025 None:intergenic
GTTGTTGATTCGTGCGACGG+CGG 0.618562 1.1:-63679730 None:intergenic
TGAGTGATGATGATGCTTCG+AGG 0.619097 1.1:+63680720 MS.gene005429:CDS
GGTGTTGAAGAAGAAGTCGT+CGG 0.621265 1.1:-63679826 None:intergenic
CTAACCGGAATAACAACTGA+AGG 0.626000 1.1:-63680224 None:intergenic
ATCGAGGATTCCGTTACGGA+GGG 0.629759 1.1:-63679847 None:intergenic
ACTTACCGGAGGAAAATTGA+CGG 0.631633 1.1:-63679974 None:intergenic
TTCACAAACTCCATAATCGA+CGG 0.638906 1.1:-63680536 None:intergenic
GAATTGAAGGGATTATGAAG+AGG 0.640801 1.1:+63680573 MS.gene005429:CDS
TTCGTGAAGAAGTTCGTGTG+AGG 0.642301 1.1:-63679691 None:intergenic
CGTCGGGTTGTTGAAGAAAG+CGG 0.644114 1.1:-63679809 None:intergenic
TGCCGTTTCGAGGGTCGTCG+TGG 0.649029 1.1:-63679623 None:intergenic
GGGATTATGAAGAGGAACCA+TGG 0.650885 1.1:+63680581 MS.gene005429:CDS
CAATACATAAACGCAACCGA+AGG 0.652956 1.1:-63680392 None:intergenic
AATCGAGGATTCCGTTACGG+AGG 0.662241 1.1:-63679848 None:intergenic
TTCCAACGAGGAAATTATCG+CGG 0.665860 1.1:+63680121 MS.gene005429:CDS
GCTTATGTTATACCTGTCGG+TGG 0.674775 1.1:+63680062 MS.gene005429:CDS
GTTATGTTGAAGCAAGCGTG+GGG 0.691208 1.1:+63680623 MS.gene005429:CDS
AAGAAAGCGGAGCTGCCAGG+TGG 0.699750 1.1:-63679796 None:intergenic
ATTTCGGCAGAACTTACCGG+AGG 0.711142 1.1:-63679985 None:intergenic
GAATGATGTTCATCATCCGG+TGG 0.719802 1.1:+63680433 MS.gene005429:CDS
GCCGTCGCACGAATCAACAA+CGG 0.725169 1.1:+63679732 MS.gene005429:CDS
ATACTCGCACACATTTCAGG+CGG 0.736142 1.1:-63680662 None:intergenic
TGGTATGGTTGTGAAAATGG+GGG 0.742084 1.1:+63680601 MS.gene005429:CDS
GTTTCCGGTTAATTCCAACG+AGG 0.797229 1.1:+63680109 MS.gene005429:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTTAGTAATTTGTATAAA+TGG + chr1.1:63680357-63680376 MS.gene005429:CDS 15.0%
!!! AAATTATAACACTGTCATTT+CGG - chr1.1:63680004-63680023 None:intergenic 20.0%
! AAGTTTGAATGTTGATGTTA+AGG + chr1.1:63680316-63680335 MS.gene005429:CDS 25.0%
! AATGTTGATGTTAAGGAAAT+TGG + chr1.1:63680323-63680342 MS.gene005429:CDS 25.0%
ATTGAATGATGTTCATCATC+CGG + chr1.1:63680430-63680449 MS.gene005429:CDS 30.0%
!! ATTATTCAGCTTTTGGAAGA+TGG + chr1.1:63680914-63680933 MS.gene005429:CDS 30.0%
!! GAGGTTAAGGTTTTATCTTA+GGG + chr1.1:63680739-63680758 MS.gene005429:CDS 30.0%
!! TATTATCAGGCTTTGAGAAA+TGG + chr1.1:63680788-63680807 MS.gene005429:CDS 30.0%
ATGGTATGGTTGTGAAAATG+GGG + chr1.1:63680600-63680619 MS.gene005429:CDS 35.0%
ATTATCGCGGAGTTTAAGAA+AGG + chr1.1:63680134-63680153 MS.gene005429:CDS 35.0%
ATTGAAGGAGGGAAGATTAA+CGG + chr1.1:63680158-63680177 MS.gene005429:CDS 35.0%
ATTGATTAGGGTTTGTAGAG+AGG + chr1.1:63680250-63680269 MS.gene005429:CDS 35.0%
ATTTCCTCGTTGGAATTAAC+CGG - chr1.1:63680116-63680135 None:intergenic 35.0%
CATGGTATGGTTGTGAAAAT+GGG + chr1.1:63680599-63680618 MS.gene005429:CDS 35.0%
CGATTATGGAGTTTGTGAAT+CGG + chr1.1:63680540-63680559 MS.gene005429:CDS 35.0%
GAAGCTTATGTTATACCTGT+CGG + chr1.1:63680059-63680078 MS.gene005429:CDS 35.0%
GAATTGAAGGGATTATGAAG+AGG + chr1.1:63680573-63680592 MS.gene005429:CDS 35.0%
GAGTTTAAGAAAGGGATTGA+AGG + chr1.1:63680143-63680162 MS.gene005429:CDS 35.0%
TAACAGCTTGATAAGCACAA+TGG - chr1.1:63680028-63680047 None:intergenic 35.0%
TGAAGTTCCTGTGTATTATC+AGG + chr1.1:63680775-63680794 MS.gene005429:CDS 35.0%
TGTGCGAGTATGATTATGAT+TGG + chr1.1:63680674-63680693 MS.gene005429:CDS 35.0%
TTAAGAAAGGGATTGAAGGA+GGG + chr1.1:63680147-63680166 MS.gene005429:CDS 35.0%
TTATCGCGGAGTTTAAGAAA+GGG + chr1.1:63680135-63680154 MS.gene005429:CDS 35.0%
TTCACAAACTCCATAATCGA+CGG - chr1.1:63680539-63680558 None:intergenic 35.0%
TTTAAGAAAGGGATTGAAGG+AGG + chr1.1:63680146-63680165 MS.gene005429:CDS 35.0%
! GACTGCAATTATTCAGCTTT+TGG + chr1.1:63680907-63680926 MS.gene005429:CDS 35.0%
! GATAAGGATGGGTTTATAAC+TGG + chr1.1:63680827-63680846 MS.gene005429:CDS 35.0%
! GTGGATCAGATTTTTGTTGA+TGG + chr1.1:63680278-63680297 MS.gene005429:CDS 35.0%
! TTCAGTTGTTATTCCGGTTA+GGG + chr1.1:63680226-63680245 MS.gene005429:CDS 35.0%
!! CGAGGTTAAGGTTTTATCTT+AGG + chr1.1:63680738-63680757 MS.gene005429:CDS 35.0%
!! TTGGTTTGCCTTCAAAGATT+CGG + chr1.1:63680693-63680712 MS.gene005429:CDS 35.0%
!!! AGTTTTGGTTTCTTTGAGTC+TGG - chr1.1:63679657-63679676 None:intergenic 35.0%
AAGAAGTTCGTGTGAGGAAA+TGG - chr1.1:63679688-63679707 None:intergenic 40.0%
ACCCTAATCAATTCCCTAAC+CGG - chr1.1:63680242-63680261 None:intergenic 40.0%
ACTTACCGGAGGAAAATTGA+CGG - chr1.1:63679977-63679996 None:intergenic 40.0%
AGATTATAGTCCTCAGCTTG+TGG + chr1.1:63680511-63680530 MS.gene005429:CDS 40.0%
ATCATACTCGCACACATTTC+AGG - chr1.1:63680668-63680687 None:intergenic 40.0%
CAATACATAAACGCAACCGA+AGG - chr1.1:63680395-63680414 None:intergenic 40.0%
CCATGGTATGGTTGTGAAAA+TGG + chr1.1:63680598-63680617 MS.gene005429:CDS 40.0%
CGAAATTTCTTCGACGTCAT+CGG - chr1.1:63679904-63679923 None:intergenic 40.0%
CTAACCGGAATAACAACTGA+AGG - chr1.1:63680227-63680246 None:intergenic 40.0%
CTCAAAGCCTGATAATACAC+AGG - chr1.1:63680785-63680804 None:intergenic 40.0%
GCCATCGTTCTTCAACAAAT+CGG + chr1.1:63679951-63679970 MS.gene005429:CDS 40.0%
GGTTTATAACTGGTTACGTG+AGG + chr1.1:63680837-63680856 MS.gene005429:CDS 40.0%
GGTTTGAAGGTGGAATTGAA+GGG + chr1.1:63680561-63680580 MS.gene005429:CDS 40.0%
TATGAAGAGGAACCATGGTA+TGG + chr1.1:63680586-63680605 MS.gene005429:CDS 40.0%
TCCGGTTAGGGAATTGATTA+GGG + chr1.1:63680238-63680257 MS.gene005429:CDS 40.0%
TCGTTGGAATTAACCGGAAA+CGG - chr1.1:63680110-63680129 None:intergenic 40.0%
TGAAAGGGATGCTAGAGATA+AGG + chr1.1:63680811-63680830 MS.gene005429:CDS 40.0%
TGAGGTTGAATTACCGTTTC+CGG + chr1.1:63680094-63680113 MS.gene005429:CDS 40.0%
TGGTATGGTTGTGAAAATGG+GGG + chr1.1:63680601-63680620 MS.gene005429:CDS 40.0%
TTACGTGAGGATTTCTCATC+AGG + chr1.1:63680850-63680869 MS.gene005429:CDS 40.0%
TTCCAACGAGGAAATTATCG+CGG + chr1.1:63680121-63680140 MS.gene005429:CDS 40.0%
TTCCGGTTAGGGAATTGATT+AGG + chr1.1:63680237-63680256 MS.gene005429:CDS 40.0%
TTTCTTTGAGTCTGGCTGAA+CGG - chr1.1:63679649-63679668 None:intergenic 40.0%
! ATGATGATGCTTCGAGGTTA+AGG + chr1.1:63680726-63680745 MS.gene005429:CDS 40.0%
! CCATTTTCACAACCATACCA+TGG - chr1.1:63680601-63680620 None:intergenic 40.0%
! CTTCAGTTGTTATTCCGGTT+AGG + chr1.1:63680225-63680244 MS.gene005429:CDS 40.0%
! GAAATGGAGTGAGTGAGTTT+TGG - chr1.1:63679672-63679691 None:intergenic 40.0%
! GATGCCTTCAGTTGTTATTC+CGG + chr1.1:63680220-63680239 MS.gene005429:CDS 40.0%
! TGATGATTTTGCGAGAATCG+AGG - chr1.1:63679866-63679885 None:intergenic 40.0%
! TGGTTTGCCTTCAAAGATTC+GGG + chr1.1:63680694-63680713 MS.gene005429:CDS 40.0%
!! AGGCTTTGAGAAATGGTGAA+AGG + chr1.1:63680795-63680814 MS.gene005429:CDS 40.0%
!! GAGTTTGTGAATCGGTTTGA+AGG + chr1.1:63680548-63680567 MS.gene005429:CDS 40.0%
!! GGCTTTGAGAAATGGTGAAA+GGG + chr1.1:63680796-63680815 MS.gene005429:CDS 40.0%
!! TTTGTGAATCGGTTTGAAGG+TGG + chr1.1:63680551-63680570 MS.gene005429:CDS 40.0%
!!! ATGGTTTTTTTCGCCACCTT+CGG + chr1.1:63680376-63680395 MS.gene005429:CDS 40.0%
AAGCGTGGGGTACAAATCTT+GGG + chr1.1:63680636-63680655 MS.gene005429:CDS 45.0%
AAGGAGGGAAGATTAACGGT+GGG + chr1.1:63680162-63680181 MS.gene005429:CDS 45.0%
AGATTAACGGTGGGAGAGTT+AGG + chr1.1:63680171-63680190 MS.gene005429:CDS 45.0%
AGGGATGCTAGAGATAAGGA+TGG + chr1.1:63680815-63680834 MS.gene005429:CDS 45.0%
ATACTCGCACACATTTCAGG+CGG - chr1.1:63680665-63680684 None:intergenic 45.0%
CGGTTTGAAGGTGGAATTGA+AGG + chr1.1:63680560-63680579 MS.gene005429:CDS 45.0%
CTCATCACCCGAATCTTTGA+AGG - chr1.1:63680704-63680723 None:intergenic 45.0%
GAATGATGTTCATCATCCGG+TGG + chr1.1:63680433-63680452 MS.gene005429:CDS 45.0%
GAGAATCGAGGATTCCGTTA+CGG - chr1.1:63679854-63679873 None:intergenic 45.0%
GCTTATGTTATACCTGTCGG+TGG + chr1.1:63680062-63680081 MS.gene005429:CDS 45.0%
GGGATGCTAGAGATAAGGAT+GGG + chr1.1:63680816-63680835 MS.gene005429:CDS 45.0%
GGGATTATGAAGAGGAACCA+TGG + chr1.1:63680581-63680600 MS.gene005429:CDS 45.0%
GGTTATGTTGAAGCAAGCGT+GGG + chr1.1:63680622-63680641 MS.gene005429:CDS 45.0%
GGTTTGTAGAGAGGAAGAAG+TGG + chr1.1:63680259-63680278 MS.gene005429:CDS 45.0%
GTCATTTCGGCAGAACTTAC+CGG - chr1.1:63679991-63680010 None:intergenic 45.0%
GTGTTGAAGAAGAAGTCGTC+GGG - chr1.1:63679828-63679847 None:intergenic 45.0%
GTTATGTTGAAGCAAGCGTG+GGG + chr1.1:63680623-63680642 MS.gene005429:CDS 45.0%
GTTTCCGGTTAATTCCAACG+AGG + chr1.1:63680109-63680128 MS.gene005429:CDS 45.0%
TACATAAACGCAACCGAAGG+TGG - chr1.1:63680392-63680411 None:intergenic 45.0%
TTCGTGAAGAAGTTCGTGTG+AGG - chr1.1:63679694-63679713 None:intergenic 45.0%
TTCTTCAACACCCTCCGTAA+CGG + chr1.1:63679837-63679856 MS.gene005429:CDS 45.0%
! GGTGTTGAAGAAGAAGTCGT+CGG - chr1.1:63679829-63679848 None:intergenic 45.0%
! TGAGTGATGATGATGCTTCG+AGG + chr1.1:63680720-63680739 MS.gene005429:CDS 45.0%
!! GCCGATTTGTTGAAGAACGA+TGG - chr1.1:63679955-63679974 None:intergenic 45.0%
!! GTTGATGGTGCTCATGCTTT+AGG + chr1.1:63680293-63680312 MS.gene005429:CDS 45.0%
AATCGAGGATTCCGTTACGG+AGG - chr1.1:63679851-63679870 None:intergenic 50.0%
ATCGAGGATTCCGTTACGGA+GGG - chr1.1:63679850-63679869 None:intergenic 50.0%
ATTTCGGCAGAACTTACCGG+AGG - chr1.1:63679988-63680007 None:intergenic 50.0%
CAAGCGTGGGGTACAAATCT+TGG + chr1.1:63680635-63680654 MS.gene005429:CDS 50.0%
CGAATCAACAACGGCAGCTT+CGG + chr1.1:63679741-63679760 MS.gene005429:CDS 50.0%
CTCCGCGATAATTTCCTCGT+TGG - chr1.1:63680126-63680145 None:intergenic 50.0%
GAAGGAGGGAAGATTAACGG+TGG + chr1.1:63680161-63680180 MS.gene005429:CDS 50.0%
GGGTTATGTTGAAGCAAGCG+TGG + chr1.1:63680621-63680640 MS.gene005429:CDS 50.0%
GTGGCGCATGAGTATGGTAA+TGG + chr1.1:63680455-63680474 MS.gene005429:CDS 50.0%
TCAATAACAGAGCCACCGAC+AGG - chr1.1:63680077-63680096 None:intergenic 50.0%
TGATGTTCATCATCCGGTGG+TGG + chr1.1:63680436-63680455 MS.gene005429:CDS 50.0%
TTGAAGAACGATGGCAGCAG+CGG - chr1.1:63679946-63679965 None:intergenic 50.0%
! ATCGGCCGTCAATTTTCCTC+CGG + chr1.1:63679969-63679988 MS.gene005429:CDS 50.0%
! CGTCGGGTTGTTGAAGAAAG+CGG - chr1.1:63679812-63679831 None:intergenic 50.0%
! TGTCGGTGGCTCTGTTATTG+AGG + chr1.1:63680076-63680095 MS.gene005429:CDS 50.0%
!! CGACGTCATCGGCATTGATT+AGG - chr1.1:63679893-63679912 None:intergenic 50.0%
!! TTTGCCTGCTGAGAGTGCTT+GGG + chr1.1:63680478-63680497 MS.gene005429:CDS 50.0%
AACCACGACGACCCTCGAAA+CGG + chr1.1:63679621-63679640 MS.gene005429:CDS 55.0%
GCCGTCGCACGAATCAACAA+CGG + chr1.1:63679732-63679751 MS.gene005429:CDS 55.0%
TTGAAGAAAGCGGAGCTGCC+AGG - chr1.1:63679802-63679821 None:intergenic 55.0%
!! CTGCTGAGAGTGCTTGGGTT+GGG + chr1.1:63680483-63680502 MS.gene005429:CDS 55.0%
!! GCCGTTGTTGATTCGTGCGA+CGG - chr1.1:63679736-63679755 None:intergenic 55.0%
!! GCTTGTGGTGCCGTCGATTA+TGG + chr1.1:63680526-63680545 MS.gene005429:CDS 55.0%
!! GTTGTTGATTCGTGCGACGG+CGG - chr1.1:63679733-63679752 None:intergenic 55.0%
!! GTTTGCCTGCTGAGAGTGCT+TGG + chr1.1:63680477-63680496 MS.gene005429:CDS 55.0%
AAGAAAGCGGAGCTGCCAGG+TGG - chr1.1:63679799-63679818 None:intergenic 60.0%
AAGAACGATGGCAGCAGCGG+TGG - chr1.1:63679943-63679962 None:intergenic 60.0%
ATCGACGGCACCACAAGCTG+AGG - chr1.1:63680524-63680543 None:intergenic 60.0%
CCAACCCAAGCACTCTCAGC+AGG - chr1.1:63680485-63680504 None:intergenic 60.0%
CCATACTCATGCGCCACCAC+CGG - chr1.1:63680452-63680471 None:intergenic 60.0%
TGATTCGTGCGACGGCGGTT+TGG - chr1.1:63679728-63679747 None:intergenic 60.0%
TTTGGGCGGTGAGGATGGAG+CGG - chr1.1:63679776-63679795 None:intergenic 60.0%
!! CCTGCTGAGAGTGCTTGGGT+TGG + chr1.1:63680482-63680501 MS.gene005429:CDS 60.0%
!! GCTGAACGGTGCCGTTTCGA+GGG - chr1.1:63679635-63679654 None:intergenic 60.0%
AACGATGGCAGCAGCGGTGG+TGG - chr1.1:63679940-63679959 None:intergenic 65.0%
AGCGGAGCTGCCAGGTGGTT+TGG - chr1.1:63679794-63679813 None:intergenic 65.0%
GCGGAGCTGCCAGGTGGTTT+GGG - chr1.1:63679793-63679812 None:intergenic 65.0%
TCCTCACCGCCCAAACCACC+TGG + chr1.1:63679781-63679800 MS.gene005429:CDS 65.0%
TTGGGCGGTGAGGATGGAGC+GGG - chr1.1:63679775-63679794 None:intergenic 65.0%
! CCGGTGGTGGCGCATGAGTA+TGG + chr1.1:63680449-63680468 MS.gene005429:CDS 65.0%
! GAGCTGCCAGGTGGTTTGGG+CGG - chr1.1:63679790-63679809 None:intergenic 65.0%
! GGTGGTTTGGGCGGTGAGGA+TGG - chr1.1:63679781-63679800 None:intergenic 65.0%
! TGCCGTTTCGAGGGTCGTCG+TGG - chr1.1:63679626-63679645 None:intergenic 65.0%
! TTCGTGCGACGGCGGTTTGG+TGG - chr1.1:63679725-63679744 None:intergenic 65.0%
!! GGCTGAACGGTGCCGTTTCG+AGG - chr1.1:63679636-63679655 None:intergenic 65.0%
!! TGCGACGGCGGTTTGGTGGT+GGG - chr1.1:63679721-63679740 None:intergenic 65.0%
TGGGCGGTGAGGATGGAGCG+GGG - chr1.1:63679774-63679793 None:intergenic 70.0%
! GCCAGGTGGTTTGGGCGGTG+AGG - chr1.1:63679785-63679804 None:intergenic 70.0%
!! GTGCGACGGCGGTTTGGTGG+TGG - chr1.1:63679722-63679741 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 63679612 63680967 63679612 ID=MS.gene005429
chr1.1 mRNA 63679612 63680967 63679612 ID=MS.gene005429.t1;Parent=MS.gene005429
chr1.1 exon 63679612 63680967 63679612 ID=MS.gene005429.t1.exon1;Parent=MS.gene005429.t1
chr1.1 CDS 63679612 63680967 63679612 ID=cds.MS.gene005429.t1;Parent=MS.gene005429.t1
Gene Sequence

>MS.gene005429

ATGGATGATAACCACGACGACCCTCGAAACGGCACCGTTCAGCCAGACTCAAAGAAACCAAAACTCACTCACTCCATTTCCTCACACGAACTTCTTCACGAATTCTCCCACCACCAAACCGCCGTCGCACGAATCAACAACGGCAGCTTCGGCAGCTGTCCCCGCTCCATCCTCACCGCCCAAACCACCTGGCAGCTCCGCTTTCTTCAACAACCCGACGACTTCTTCTTCAACACCCTCCGTAACGGAATCCTCGATTCTCGCAAAATCATCAAGAACCTAATCAATGCCGATGACGTCGAAGAAATTTCGTTAATCGATAATGCCACCACCGCTGCTGCCATCGTTCTTCAACAAATCGGCCGTCAATTTTCCTCCGGTAAGTTCTGCCGAAATGACAGTGTTATAATTTTCCATTGTGCTTATCAAGCTGTTAAGAAATCAATTGAAGCTTATGTTATACCTGTCGGTGGCTCTGTTATTGAGGTTGAATTACCGTTTCCGGTTAATTCCAACGAGGAAATTATCGCGGAGTTTAAGAAAGGGATTGAAGGAGGGAAGATTAACGGTGGGAGAGTTAGGTTAGCGATAATTGATCACATTACATCGATGCCTTCAGTTGTTATTCCGGTTAGGGAATTGATTAGGGTTTGTAGAGAGGAAGAAGTGGATCAGATTTTTGTTGATGGTGCTCATGCTTTAGGAAGTTTGAATGTTGATGTTAAGGAAATTGGTGCTGATTTTTATGTTAGTAATTTGTATAAATGGTTTTTTTCGCCACCTTCGGTTGCGTTTATGTATTGTAATAAGAATAAGAAATTGAATGATGTTCATCATCCGGTGGTGGCGCATGAGTATGGTAATGGTTTGCCTGCTGAGAGTGCTTGGGTTGGGATGAGAGATTATAGTCCTCAGCTTGTGGTGCCGTCGATTATGGAGTTTGTGAATCGGTTTGAAGGTGGAATTGAAGGGATTATGAAGAGGAACCATGGTATGGTTGTGAAAATGGGGGTTATGTTGAAGCAAGCGTGGGGTACAAATCTTGGGTCACCGCCTGAAATGTGTGCGAGTATGATTATGATTGGTTTGCCTTCAAAGATTCGGGTGATGAGTGATGATGATGCTTCGAGGTTAAGGTTTTATCTTAGGGTTTATCATGCTATTGAAGTTCCTGTGTATTATCAGGCTTTGAGAAATGGTGAAAGGGATGCTAGAGATAAGGATGGGTTTATAACTGGTTACGTGAGGATTTCTCATCAGGTGTATAACACTGTTGATGATTATAATAGACTTAAGACTGCAATTATTCAGCTTTTGGAAGATGGAAAAATTTGCAGTGAACTTCCTAAAGAGTGA
Protein sequence

>MS.gene005429.t1

MDDNHDDPRNGTVQPDSKKPKLTHSISSHELLHEFSHHQTAVARINNGSFGSCPRSILTAQTTWQLRFLQQPDDFFFNTLRNGILDSRKIIKNLINADDVEEISLIDNATTAAAIVLQQIGRQFSSGKFCRNDSVIIFHCAYQAVKKSIEAYVIPVGGSVIEVELPFPVNSNEEIIAEFKKGIEGGKINGGRVRLAIIDHITSMPSVVIPVRELIRVCREEEVDQIFVDGAHALGSLNVDVKEIGADFYVSNLYKWFFSPPSVAFMYCNKNKKLNDVHHPVVAHEYGNGLPAESAWVGMRDYSPQLVVPSIMEFVNRFEGGIEGIMKRNHGMVVKMGVMLKQAWGTNLGSPPEMCASMIMIGLPSKIRVMSDDDASRLRFYLRVYHAIEVPVYYQALRNGERDARDKDGFITGYVRISHQVYNTVDDYNRLKTAIIQLLEDGKICSELPKE