Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072597.t1 | XP_003626145.1 | 97.8 | 455 | 8 | 2 | 1 | 453 | 1 | 455 | 1.30E-261 | 911.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072597.t1 | Q3E6S9 | 71.9 | 430 | 121 | 0 | 23 | 452 | 45 | 474 | 2.7e-191 | 669.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072597.t1 | G7KWF1 | 97.8 | 455 | 8 | 2 | 1 | 453 | 1 | 455 | 9.4e-262 | 911.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050796 | MS.gene072597 | 0.811034 | 8.57E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene20907 | MS.gene072597 | PPI |
| MS.gene20906 | MS.gene072597 | PPI |
| MS.gene07363 | MS.gene072597 | PPI |
| MS.gene005429 | MS.gene072597 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072597.t1 | MTR_7g111540 | 97.802 | 455 | 8 | 2 | 1 | 453 | 1 | 455 | 0.0 | 928 |
| MS.gene072597.t1 | MTR_1g484920 | 71.018 | 452 | 116 | 7 | 4 | 452 | 5 | 444 | 0.0 | 668 |
| MS.gene072597.t1 | MTR_1g484920 | 63.778 | 450 | 109 | 7 | 4 | 452 | 5 | 401 | 0.0 | 582 |
| MS.gene072597.t1 | MTR_1g086070 | 60.000 | 445 | 167 | 4 | 16 | 453 | 9 | 449 | 0.0 | 559 |
| MS.gene072597.t1 | MTR_3g083980 | 24.291 | 247 | 157 | 9 | 52 | 286 | 79 | 307 | 1.31e-11 | 66.2 |
| MS.gene072597.t1 | MTR_3g083980 | 24.291 | 247 | 157 | 9 | 52 | 286 | 79 | 307 | 2.35e-11 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072597.t1 | AT5G26600 | 71.860 | 430 | 121 | 0 | 23 | 452 | 45 | 474 | 0.0 | 660 |
| MS.gene072597.t1 | AT5G26600 | 71.860 | 430 | 121 | 0 | 23 | 452 | 45 | 474 | 0.0 | 660 |
| MS.gene072597.t1 | AT3G62130 | 59.688 | 449 | 170 | 3 | 10 | 451 | 7 | 451 | 0.0 | 585 |
| MS.gene072597.t1 | AT3G62130 | 59.688 | 449 | 170 | 3 | 10 | 451 | 7 | 451 | 0.0 | 585 |
Find 0 sgRNAs with CRISPR-Local
Find 141 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATTGGAAGAAGATAGTTT+AGG | + | 50592:9100-9119 | None:intergenic | 25.0% |
| ! | ACTAAAAAGAATCCTAAAAC+TGG | - | 50592:9847-9866 | MS.gene072597:CDS | 25.0% |
| ! | TTCTTATCAGGTTTACAATA+AGG | - | 50592:10284-10303 | MS.gene072597:CDS | 25.0% |
| !! | ATACTAGTAATTTGCATAAG+TGG | - | 50592:9791-9810 | MS.gene072597:CDS | 25.0% |
| !!! | AAGATAGTTTAGGTTTTTTG+GGG | + | 50592:9090-9109 | None:intergenic | 25.0% |
| !!! | AGAAGATAGTTTAGGTTTTT+TGG | + | 50592:9092-9111 | None:intergenic | 25.0% |
| !!! | GAAGATAGTTTAGGTTTTTT+GGG | + | 50592:9091-9110 | None:intergenic | 25.0% |
| !!! | TTAGAAAAGCTTTGGAAAAA+GGG | - | 50592:9581-9600 | MS.gene072597:CDS | 25.0% |
| !!! | TTTAGAAAAGCTTTGGAAAA+AGG | - | 50592:9580-9599 | MS.gene072597:CDS | 25.0% |
| AAAAAGAATCCTAAAACTGG+TGG | - | 50592:9850-9869 | MS.gene072597:CDS | 30.0% | |
| ATACTAGCACACATATGTTT+CGG | + | 50592:10105-10124 | None:intergenic | 30.0% | |
| CTTTGTTGAATTTACCTTCA+CGG | + | 50592:9420-9439 | None:intergenic | 30.0% | |
| GACTAAGTTTAGAAAAGCTT+TGG | - | 50592:9573-9592 | MS.gene072597:CDS | 30.0% | |
| GATGTTGATATGCAAGATAT+TGG | - | 50592:9757-9776 | MS.gene072597:CDS | 30.0% | |
| GTTATGCTTCATTATGCTTA+CGG | - | 50592:9451-9470 | MS.gene072597:CDS | 30.0% | |
| TGGGAAGAAAATTAGATTAG+CGG | - | 50592:9612-9631 | MS.gene072597:CDS | 30.0% | |
| TTGAGGACTATTACAAGTTT+CGG | - | 50592:10307-10326 | MS.gene072597:CDS | 30.0% | |
| ! | GATTTTGTGAATAGGTTTGA+AGG | - | 50592:9988-10007 | MS.gene072597:CDS | 30.0% |
| !!! | AGATAGTTTAGGTTTTTTGG+GGG | + | 50592:9089-9108 | None:intergenic | 30.0% |
| AAGGGATTATAGTGCTCAAT+TGG | - | 50592:9948-9967 | MS.gene072597:CDS | 35.0% | |
| ATCTGAATCAATTCCTTCAC+AGG | + | 50592:9676-9695 | None:intergenic | 35.0% | |
| CATGAAGCTGTTATTGAGAT+GGG | - | 50592:10039-10058 | MS.gene072597:CDS | 35.0% | |
| CGTGAAGGTAAATTCAACAA+AGG | - | 50592:9418-9437 | MS.gene072597:CDS | 35.0% | |
| CTAGGCGGTCTATAATATAT+AGG | + | 50592:10225-10244 | None:intergenic | 35.0% | |
| GAAACATATGTGTGCTAGTA+TGG | - | 50592:10104-10123 | MS.gene072597:CDS | 35.0% | |
| GTATGCAAGGATTTCTTATC+AGG | - | 50592:10272-10291 | MS.gene072597:CDS | 35.0% | |
| TATGTGTGCTAGTATGGTTA+TGG | - | 50592:10110-10129 | MS.gene072597:CDS | 35.0% | |
| TCAGGTTTACAATAAGGTTG+AGG | - | 50592:10290-10309 | MS.gene072597:CDS | 35.0% | |
| TCATGAAGCTGTTATTGAGA+TGG | - | 50592:10038-10057 | MS.gene072597:CDS | 35.0% | |
| TGAGGACTATTACAAGTTTC+GGG | - | 50592:10308-10327 | MS.gene072597:CDS | 35.0% | |
| TTGGAAAAAGGGAAAGTTGA+TGG | - | 50592:9592-9611 | MS.gene072597:CDS | 35.0% | |
| TTGTGAATAGGTTTGAAGGA+GGG | - | 50592:9992-10011 | MS.gene072597:CDS | 35.0% | |
| TTTGTGAATAGGTTTGAAGG+AGG | - | 50592:9991-10010 | MS.gene072597:CDS | 35.0% | |
| ! | ACACATTTGAGGGATGTTTT+TGG | - | 50592:10189-10208 | MS.gene072597:CDS | 35.0% |
| ! | ATTGATTCAGATTTGCAGAG+AGG | - | 50592:9684-9703 | MS.gene072597:CDS | 35.0% |
| ! | TGGAAAAAGGGAAAGTTGAT+GGG | - | 50592:9593-9612 | MS.gene072597:CDS | 35.0% |
| ! | TTAACCAACTGGTGGATAAT+GGG | - | 50592:10337-10356 | MS.gene072597:CDS | 35.0% |
| ! | TTCGGATTCAATTTCTGAAG+CGG | + | 50592:9128-9147 | None:intergenic | 35.0% |
| !!! | AGGCTTTGGATTTTGTGAAT+AGG | - | 50592:9980-9999 | MS.gene072597:CDS | 35.0% |
| !!! | CTAGGTTTTGGATAATTGAG+CGG | + | 50592:9309-9328 | None:intergenic | 35.0% |
| !!! | TAGGTTTTGGATAATTGAGC+GGG | + | 50592:9308-9327 | None:intergenic | 35.0% |
| !!! | TTGGTTTGCCTACTTGTTTT+GGG | - | 50592:10133-10152 | MS.gene072597:CDS | 35.0% |
| AAGAATCCTAAAACTGGTGG+TGG | - | 50592:9853-9872 | MS.gene072597:CDS | 40.0% | |
| ATTCAGATTTGCAGAGAGGA+AGG | - | 50592:9688-9707 | MS.gene072597:CDS | 40.0% | |
| ATTCTGATGCACTTAAGCTG+AGG | - | 50592:10166-10185 | MS.gene072597:CDS | 40.0% | |
| CTTCTAACCCCAAAACAAGT+AGG | + | 50592:10144-10163 | None:intergenic | 40.0% | |
| GGATTATAGTGCTCAATTGG+TGG | - | 50592:9951-9970 | MS.gene072597:CDS | 40.0% | |
| GGATTGAAGGGATTAAGAAG+AGG | - | 50592:10013-10032 | MS.gene072597:CDS | 40.0% | |
| GTATACAAAAACGCGATCGA+AGG | + | 50592:9829-9848 | None:intergenic | 40.0% | |
| GTGGTTGAAGTAGAAGTGAT+CGG | + | 50592:9263-9282 | None:intergenic | 40.0% | |
| GTGTCTCATGAGTATGGAAA+TGG | - | 50592:9895-9914 | MS.gene072597:CDS | 40.0% | |
| TAAGCTGAGGACACATTTGA+GGG | - | 50592:10179-10198 | MS.gene072597:CDS | 40.0% | |
| TATTATAGACCGCCTAGAGA+TGG | - | 50592:10228-10247 | MS.gene072597:CDS | 40.0% | |
| TATTGAGATGGGAGAGATGT+TGG | - | 50592:10050-10069 | MS.gene072597:CDS | 40.0% | |
| TGCTGTTAAGAAATCCATGG+AGG | - | 50592:9474-9493 | MS.gene072597:CDS | 40.0% | |
| TGGTTGAAGTAGAAGTGATC+GGG | + | 50592:9262-9281 | None:intergenic | 40.0% | |
| TGTGCTAGTATGGTTATGGT+TGG | - | 50592:10114-10133 | MS.gene072597:CDS | 40.0% | |
| TTAAGCTGAGGACACATTTG+AGG | - | 50592:10178-10197 | MS.gene072597:CDS | 40.0% | |
| TTCACAAAATCCAAAGCCTC+AGG | + | 50592:9979-9998 | None:intergenic | 40.0% | |
| TTCACAGGAATCACAACACA+AGG | + | 50592:9661-9680 | None:intergenic | 40.0% | |
| TTGTGTTGTGATTCCTGTGA+AGG | - | 50592:9660-9679 | MS.gene072597:CDS | 40.0% | |
| ! | AAATCACCACCACCAGTTTT+AGG | + | 50592:9862-9881 | None:intergenic | 40.0% |
| ! | AGAGATGTTGGTGAAAGCTT+GGG | - | 50592:10062-10081 | MS.gene072597:CDS | 40.0% |
| ! | GTTAACCAACTGGTGGATAA+TGG | - | 50592:10336-10355 | MS.gene072597:CDS | 40.0% |
| ! | TGAAGTTCCGTTACCTTTTC+CGG | - | 50592:9528-9547 | MS.gene072597:CDS | 40.0% |
| !! | GTATGGAAATGGATTAGCTG+TGG | - | 50592:9906-9925 | MS.gene072597:CDS | 40.0% |
| !! | GTGGTGATTGTTTTTCTCAG+AGG | + | 50592:9032-9051 | None:intergenic | 40.0% |
| !! | TGATGTCTTTAGCGTTGACT+AGG | + | 50592:9327-9346 | None:intergenic | 40.0% |
| !! | TTCTGAAGCGGTGATGAAAT+TGG | + | 50592:9116-9135 | None:intergenic | 40.0% |
| !!! | AGTTTAGGTTTTTTGGGGGT+GGG | + | 50592:9085-9104 | None:intergenic | 40.0% |
| !!! | CTTTAGCGTTGACTAGGTTT+TGG | + | 50592:9321-9340 | None:intergenic | 40.0% |
| !!! | GTTGGTTTGCCTACTTGTTT+TGG | - | 50592:10132-10151 | MS.gene072597:CDS | 40.0% |
| !!! | TAGTTTAGGTTTTTTGGGGG+TGG | + | 50592:9086-9105 | None:intergenic | 40.0% |
| !!! | TGGTTTGCCTACTTGTTTTG+GGG | - | 50592:10134-10153 | MS.gene072597:CDS | 40.0% |
| AAAGCTTGGGGAACTCATCT+TGG | - | 50592:10075-10094 | MS.gene072597:CDS | 45.0% | |
| AAGCTTGGGGAACTCATCTT+GGG | - | 50592:10076-10095 | MS.gene072597:CDS | 45.0% | |
| AATTGAGCGGGAGTTGTTGA+TGG | + | 50592:9296-9315 | None:intergenic | 45.0% | |
| AGGTTTGAAGGAGGGATTGA+AGG | - | 50592:10000-10019 | MS.gene072597:CDS | 45.0% | |
| ATCTCATCGTTCGAACTCAC+CGG | + | 50592:9550-9569 | None:intergenic | 45.0% | |
| CAAACCCATTATCCACCAGT+TGG | + | 50592:10344-10363 | None:intergenic | 45.0% | |
| CCATACTCATGAGACACAAC+AGG | + | 50592:9892-9911 | None:intergenic | 45.0% | |
| CCTGTTGTGTCTCATGAGTA+TGG | - | 50592:9889-9908 | MS.gene072597:CDS | 45.0% | |
| CGTTCAATCCCAGACATCAA+CGG | - | 50592:9052-9071 | MS.gene072597:CDS | 45.0% | |
| GAACTCACCGGAAAAGGTAA+CGG | + | 50592:9538-9557 | None:intergenic | 45.0% | |
| GGATCAACTTCTCCATCTCT+AGG | + | 50592:10243-10262 | None:intergenic | 45.0% | |
| GGTGTGTTGAAGAACGATAG+CGG | + | 50592:9386-9405 | None:intergenic | 45.0% | |
| GGTTTGAAGGAGGGATTGAA+GGG | - | 50592:10001-10020 | MS.gene072597:CDS | 45.0% | |
| GTTGATGCTGCTCATGCAAT+TGG | - | 50592:9727-9746 | MS.gene072597:CDS | 45.0% | |
| TACAAAAACGCGATCGAAGG+TGG | + | 50592:9826-9845 | None:intergenic | 45.0% | |
| TCAACTTCTCCATCTCTAGG+CGG | + | 50592:10240-10259 | None:intergenic | 45.0% | |
| ! | CGTTGATGTCTGGGATTGAA+CGG | + | 50592:9054-9073 | None:intergenic | 45.0% |
| ! | GAGAGATGTTGGTGAAAGCT+TGG | - | 50592:10061-10080 | MS.gene072597:CDS | 45.0% |
| ! | GAGATGTTGGTGAAAGCTTG+GGG | - | 50592:10063-10082 | MS.gene072597:CDS | 45.0% |
| ! | TGGAGAAGTTGATCCTGTGA+CGG | - | 50592:10248-10267 | MS.gene072597:CDS | 45.0% |
| !! | AAAGTGCTTGGATTGGCACA+AGG | - | 50592:9929-9948 | MS.gene072597:CDS | 45.0% |
| !! | AAGTGCTTGGATTGGCACAA+GGG | - | 50592:9930-9949 | MS.gene072597:CDS | 45.0% |
| !! | CGGTGCTGTTAAGAAATCCA+TGG | - | 50592:9471-9490 | MS.gene072597:CDS | 45.0% |
| !! | GATTAGCTGTGGAAAGTGCT+TGG | - | 50592:9917-9936 | MS.gene072597:CDS | 45.0% |
| !!! | GTTTAGGTTTTTTGGGGGTG+GGG | + | 50592:9084-9103 | None:intergenic | 45.0% |
| ACCTTCACGGAAACACCAAG+CGG | + | 50592:9407-9426 | None:intergenic | 50.0% | |
| CGCATTAACAACGGCAGCTT+TGG | - | 50592:9175-9194 | MS.gene072597:CDS | 50.0% | |
| GGATGCTGTTAACCAACTGG+TGG | - | 50592:10329-10348 | MS.gene072597:CDS | 50.0% | |
| TCCTCTCCGCTCAACAACAA+TGG | - | 50592:9215-9234 | MS.gene072597:CDS | 50.0% | |
| TCGGGATGCTGTTAACCAAC+TGG | - | 50592:10326-10345 | MS.gene072597:CDS | 50.0% | |
| TCGTTCGAACTCACCGGAAA+AGG | + | 50592:9544-9563 | None:intergenic | 50.0% | |
| TCGTTCTTCAACACACCGCT+TGG | - | 50592:9389-9408 | MS.gene072597:CDS | 50.0% | |
| ! | ATTGGTGGTTCCTGAGGCTT+TGG | - | 50592:9966-9985 | MS.gene072597:CDS | 50.0% |
| ! | GAGAAGTTGATCCTGTGACG+GGG | - | 50592:10250-10269 | MS.gene072597:CDS | 50.0% |
| ! | GAGCTGCCATTGTTGTTGAG+CGG | + | 50592:9224-9243 | None:intergenic | 50.0% |
| ! | GGAGAAGTTGATCCTGTGAC+GGG | - | 50592:10249-10268 | MS.gene072597:CDS | 50.0% |
| ! | TGATGTCTGGGATTGAACGG+TGG | + | 50592:9051-9070 | None:intergenic | 50.0% |
| ! | TGCTCAATTGGTGGTTCCTG+AGG | - | 50592:9960-9979 | MS.gene072597:CDS | 50.0% |
| ! | TTGAAGAACGATAGCGGCAG+CGG | + | 50592:9380-9399 | None:intergenic | 50.0% |
| ! | TTGTTGTTGAGCGGAGAGGA+TGG | + | 50592:9215-9234 | None:intergenic | 50.0% |
| !! | AGTTGTTGATGGCGGGTTTG+AGG | + | 50592:9285-9304 | None:intergenic | 50.0% |
| !! | CAGAGAGGAAGGTGTTGAAC+AGG | - | 50592:9699-9718 | MS.gene072597:CDS | 50.0% |
| !! | GCTGTGGAAAGTGCTTGGAT+TGG | - | 50592:9922-9941 | MS.gene072597:CDS | 50.0% |
| ACGAGTGACGTAAGCCTCCA+TGG | + | 50592:9491-9510 | None:intergenic | 55.0% | |
| ATCCTTGCATACCCCGTCAC+AGG | + | 50592:10264-10283 | None:intergenic | 55.0% | |
| GCCGTCGCTCGCATTAACAA+CGG | - | 50592:9166-9185 | MS.gene072597:CDS | 55.0% | |
| GCCGTTGTTAATGCGAGCGA+CGG | + | 50592:9170-9189 | None:intergenic | 55.0% | |
| GTTGTTAATGCGAGCGACGG+CGG | + | 50592:9167-9186 | None:intergenic | 55.0% | |
| TTGTTGAGCGGAGAGGATGG+AGG | + | 50592:9212-9231 | None:intergenic | 55.0% | |
| ! | ATCCTGTGACGGGGTATGCA+AGG | - | 50592:10259-10278 | MS.gene072597:CDS | 55.0% |
| ! | CTTACGTCACTCGTGCTGGT+GGG | - | 50592:9497-9516 | MS.gene072597:CDS | 55.0% |
| ! | GCCATTGTTGTTGAGCGGAG+AGG | + | 50592:9219-9238 | None:intergenic | 55.0% |
| ! | GGTGTGTGCCGTTGATGTCT+GGG | + | 50592:9063-9082 | None:intergenic | 55.0% |
| ! | TGAGCGGGAGTTGTTGATGG+CGG | + | 50592:9293-9312 | None:intergenic | 55.0% |
| !! | ACCGCTTGGTGTTTCCGTGA+AGG | - | 50592:9403-9422 | MS.gene072597:CDS | 55.0% |
| !! | TGTTGATGGCGGGTTTGAGG+TGG | + | 50592:9282-9301 | None:intergenic | 55.0% |
| GAGGCTTACGTCACTCGTGC+TGG | - | 50592:9493-9512 | MS.gene072597:CDS | 60.0% | |
| GCTTACGTCACTCGTGCTGG+TGG | - | 50592:9496-9515 | MS.gene072597:CDS | 60.0% | |
| GGAGAGGATGGAGGAAGGAC+AGG | + | 50592:9203-9222 | None:intergenic | 60.0% | |
| GGCGTCGTGGTGGCTGAATT+CGG | + | 50592:9146-9165 | None:intergenic | 60.0% | |
| TGAGCGGAGAGGATGGAGGA+AGG | + | 50592:9208-9227 | None:intergenic | 60.0% | |
| ! | AAGAACGATAGCGGCAGCGG+TGG | + | 50592:9377-9396 | None:intergenic | 60.0% |
| ! | GGGTGTGTGCCGTTGATGTC+TGG | + | 50592:9064-9083 | None:intergenic | 60.0% |
| !! | GAGCGGGAGTTGTTGATGGC+GGG | + | 50592:9292-9311 | None:intergenic | 60.0% |
| ! | AACGATAGCGGCAGCGGTGG+TGG | + | 50592:9374-9393 | None:intergenic | 65.0% |
| ! | GAGCGACGGCGGCGTCGTGG+TGG | + | 50592:9156-9175 | None:intergenic | 80.0% |
| ! | TGCGAGCGACGGCGGCGTCG+TGG | + | 50592:9159-9178 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 50592 | gene | 9025 | 10386 | 9025 | ID=MS.gene072597 |
| 50592 | mRNA | 9025 | 10386 | 9025 | ID=MS.gene072597.t1;Parent=MS.gene072597 |
| 50592 | exon | 9025 | 10386 | 9025 | ID=MS.gene072597.t1.exon1;Parent=MS.gene072597.t1 |
| 50592 | CDS | 9025 | 10386 | 9025 | ID=cds.MS.gene072597.t1;Parent=MS.gene072597.t1 |
| Gene Sequence |
| Protein sequence |
