Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072599.t1 | XP_003626145.1 | 97.8 | 455 | 8 | 2 | 1 | 453 | 1 | 455 | 1.30E-261 | 911.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072599.t1 | Q3E6S9 | 71.9 | 430 | 121 | 0 | 23 | 452 | 45 | 474 | 2.7e-191 | 669.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072599.t1 | G7KWF1 | 97.8 | 455 | 8 | 2 | 1 | 453 | 1 | 455 | 9.4e-262 | 911.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050796 | MS.gene072599 | 0.809634 | 1.72E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene20907 | MS.gene072599 | PPI |
| MS.gene005429 | MS.gene072599 | PPI |
| MS.gene20906 | MS.gene072599 | PPI |
| MS.gene07363 | MS.gene072599 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072599.t1 | MTR_7g111540 | 97.802 | 455 | 8 | 2 | 1 | 453 | 1 | 455 | 0.0 | 928 |
| MS.gene072599.t1 | MTR_1g484920 | 71.018 | 452 | 116 | 7 | 4 | 452 | 5 | 444 | 0.0 | 668 |
| MS.gene072599.t1 | MTR_1g484920 | 63.778 | 450 | 109 | 7 | 4 | 452 | 5 | 401 | 0.0 | 582 |
| MS.gene072599.t1 | MTR_1g086070 | 60.000 | 445 | 167 | 4 | 16 | 453 | 9 | 449 | 0.0 | 559 |
| MS.gene072599.t1 | MTR_3g083980 | 24.291 | 247 | 157 | 9 | 52 | 286 | 79 | 307 | 1.31e-11 | 66.2 |
| MS.gene072599.t1 | MTR_3g083980 | 24.291 | 247 | 157 | 9 | 52 | 286 | 79 | 307 | 2.35e-11 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072599.t1 | AT5G26600 | 71.860 | 430 | 121 | 0 | 23 | 452 | 45 | 474 | 0.0 | 660 |
| MS.gene072599.t1 | AT5G26600 | 71.860 | 430 | 121 | 0 | 23 | 452 | 45 | 474 | 0.0 | 660 |
| MS.gene072599.t1 | AT3G62130 | 59.688 | 449 | 170 | 3 | 10 | 451 | 7 | 451 | 0.0 | 585 |
| MS.gene072599.t1 | AT3G62130 | 59.688 | 449 | 170 | 3 | 10 | 451 | 7 | 451 | 0.0 | 585 |
Find 0 sgRNAs with CRISPR-Local
Find 141 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATTGGAAGAAGATAGTTT+AGG | + | 50598:4693-4712 | None:intergenic | 25.0% |
| ! | ACTAAAAAGAATCCTAAAAC+TGG | - | 50598:5440-5459 | MS.gene072599:CDS | 25.0% |
| ! | TTCTTATCAGGTTTACAATA+AGG | - | 50598:5877-5896 | MS.gene072599:CDS | 25.0% |
| !! | ATACTAGTAATTTGCATAAG+TGG | - | 50598:5384-5403 | MS.gene072599:CDS | 25.0% |
| !!! | AAGATAGTTTAGGTTTTTTG+GGG | + | 50598:4683-4702 | None:intergenic | 25.0% |
| !!! | AGAAGATAGTTTAGGTTTTT+TGG | + | 50598:4685-4704 | None:intergenic | 25.0% |
| !!! | GAAGATAGTTTAGGTTTTTT+GGG | + | 50598:4684-4703 | None:intergenic | 25.0% |
| !!! | TTAGAAAAGCTTTGGAAAAA+GGG | - | 50598:5174-5193 | MS.gene072599:CDS | 25.0% |
| !!! | TTTAGAAAAGCTTTGGAAAA+AGG | - | 50598:5173-5192 | MS.gene072599:CDS | 25.0% |
| AAAAAGAATCCTAAAACTGG+TGG | - | 50598:5443-5462 | MS.gene072599:CDS | 30.0% | |
| ATACTAGCACACATATGTTT+CGG | + | 50598:5698-5717 | None:intergenic | 30.0% | |
| CTTTGTTGAATTTACCTTCA+CGG | + | 50598:5013-5032 | None:intergenic | 30.0% | |
| GACTAAGTTTAGAAAAGCTT+TGG | - | 50598:5166-5185 | MS.gene072599:CDS | 30.0% | |
| GATGTTGATATGCAAGATAT+TGG | - | 50598:5350-5369 | MS.gene072599:CDS | 30.0% | |
| GTTATGCTTCATTATGCTTA+CGG | - | 50598:5044-5063 | MS.gene072599:CDS | 30.0% | |
| TGGGAAGAAAATTAGATTAG+CGG | - | 50598:5205-5224 | MS.gene072599:CDS | 30.0% | |
| TTGAGGACTATTACAAGTTT+CGG | - | 50598:5900-5919 | MS.gene072599:CDS | 30.0% | |
| ! | GATTTTGTGAATAGGTTTGA+AGG | - | 50598:5581-5600 | MS.gene072599:CDS | 30.0% |
| !!! | AGATAGTTTAGGTTTTTTGG+GGG | + | 50598:4682-4701 | None:intergenic | 30.0% |
| AAGGGATTATAGTGCTCAAT+TGG | - | 50598:5541-5560 | MS.gene072599:CDS | 35.0% | |
| ATCTGAATCAATTCCTTCAC+AGG | + | 50598:5269-5288 | None:intergenic | 35.0% | |
| CATGAAGCTGTTATTGAGAT+GGG | - | 50598:5632-5651 | MS.gene072599:CDS | 35.0% | |
| CGTGAAGGTAAATTCAACAA+AGG | - | 50598:5011-5030 | MS.gene072599:CDS | 35.0% | |
| CTAGGCGGTCTATAATATAT+AGG | + | 50598:5818-5837 | None:intergenic | 35.0% | |
| GAAACATATGTGTGCTAGTA+TGG | - | 50598:5697-5716 | MS.gene072599:CDS | 35.0% | |
| GTATGCAAGGATTTCTTATC+AGG | - | 50598:5865-5884 | MS.gene072599:CDS | 35.0% | |
| TATGTGTGCTAGTATGGTTA+TGG | - | 50598:5703-5722 | MS.gene072599:CDS | 35.0% | |
| TCAGGTTTACAATAAGGTTG+AGG | - | 50598:5883-5902 | MS.gene072599:CDS | 35.0% | |
| TCATGAAGCTGTTATTGAGA+TGG | - | 50598:5631-5650 | MS.gene072599:CDS | 35.0% | |
| TGAGGACTATTACAAGTTTC+GGG | - | 50598:5901-5920 | MS.gene072599:CDS | 35.0% | |
| TTGGAAAAAGGGAAAGTTGA+TGG | - | 50598:5185-5204 | MS.gene072599:CDS | 35.0% | |
| TTGTGAATAGGTTTGAAGGA+GGG | - | 50598:5585-5604 | MS.gene072599:CDS | 35.0% | |
| TTTGTGAATAGGTTTGAAGG+AGG | - | 50598:5584-5603 | MS.gene072599:CDS | 35.0% | |
| ! | ACACATTTGAGGGATGTTTT+TGG | - | 50598:5782-5801 | MS.gene072599:CDS | 35.0% |
| ! | ATTGATTCAGATTTGCAGAG+AGG | - | 50598:5277-5296 | MS.gene072599:CDS | 35.0% |
| ! | TGGAAAAAGGGAAAGTTGAT+GGG | - | 50598:5186-5205 | MS.gene072599:CDS | 35.0% |
| ! | TTAACCAACTGGTGGATAAT+GGG | - | 50598:5930-5949 | MS.gene072599:CDS | 35.0% |
| ! | TTCGGATTCAATTTCTGAAG+CGG | + | 50598:4721-4740 | None:intergenic | 35.0% |
| !!! | AGGCTTTGGATTTTGTGAAT+AGG | - | 50598:5573-5592 | MS.gene072599:CDS | 35.0% |
| !!! | CTAGGTTTTGGATAATTGAG+CGG | + | 50598:4902-4921 | None:intergenic | 35.0% |
| !!! | TAGGTTTTGGATAATTGAGC+GGG | + | 50598:4901-4920 | None:intergenic | 35.0% |
| !!! | TTGGTTTGCCTACTTGTTTT+GGG | - | 50598:5726-5745 | MS.gene072599:CDS | 35.0% |
| AAGAATCCTAAAACTGGTGG+TGG | - | 50598:5446-5465 | MS.gene072599:CDS | 40.0% | |
| ATTCAGATTTGCAGAGAGGA+AGG | - | 50598:5281-5300 | MS.gene072599:CDS | 40.0% | |
| ATTCTGATGCACTTAAGCTG+AGG | - | 50598:5759-5778 | MS.gene072599:CDS | 40.0% | |
| CTTCTAACCCCAAAACAAGT+AGG | + | 50598:5737-5756 | None:intergenic | 40.0% | |
| GGATTATAGTGCTCAATTGG+TGG | - | 50598:5544-5563 | MS.gene072599:CDS | 40.0% | |
| GGATTGAAGGGATTAAGAAG+AGG | - | 50598:5606-5625 | MS.gene072599:CDS | 40.0% | |
| GTATACAAAAACGCGATCGA+AGG | + | 50598:5422-5441 | None:intergenic | 40.0% | |
| GTGGTTGAAGTAGAAGTGAT+CGG | + | 50598:4856-4875 | None:intergenic | 40.0% | |
| GTGTCTCATGAGTATGGAAA+TGG | - | 50598:5488-5507 | MS.gene072599:CDS | 40.0% | |
| TAAGCTGAGGACACATTTGA+GGG | - | 50598:5772-5791 | MS.gene072599:CDS | 40.0% | |
| TATTATAGACCGCCTAGAGA+TGG | - | 50598:5821-5840 | MS.gene072599:CDS | 40.0% | |
| TATTGAGATGGGAGAGATGT+TGG | - | 50598:5643-5662 | MS.gene072599:CDS | 40.0% | |
| TGCTGTTAAGAAATCCATGG+AGG | - | 50598:5067-5086 | MS.gene072599:CDS | 40.0% | |
| TGGTTGAAGTAGAAGTGATC+GGG | + | 50598:4855-4874 | None:intergenic | 40.0% | |
| TGTGCTAGTATGGTTATGGT+TGG | - | 50598:5707-5726 | MS.gene072599:CDS | 40.0% | |
| TTAAGCTGAGGACACATTTG+AGG | - | 50598:5771-5790 | MS.gene072599:CDS | 40.0% | |
| TTCACAAAATCCAAAGCCTC+AGG | + | 50598:5572-5591 | None:intergenic | 40.0% | |
| TTCACAGGAATCACAACACA+AGG | + | 50598:5254-5273 | None:intergenic | 40.0% | |
| TTGTGTTGTGATTCCTGTGA+AGG | - | 50598:5253-5272 | MS.gene072599:CDS | 40.0% | |
| ! | AAATCACCACCACCAGTTTT+AGG | + | 50598:5455-5474 | None:intergenic | 40.0% |
| ! | AGAGATGTTGGTGAAAGCTT+GGG | - | 50598:5655-5674 | MS.gene072599:CDS | 40.0% |
| ! | GTTAACCAACTGGTGGATAA+TGG | - | 50598:5929-5948 | MS.gene072599:CDS | 40.0% |
| ! | TGAAGTTCCGTTACCTTTTC+CGG | - | 50598:5121-5140 | MS.gene072599:CDS | 40.0% |
| !! | GTATGGAAATGGATTAGCTG+TGG | - | 50598:5499-5518 | MS.gene072599:CDS | 40.0% |
| !! | GTGGTGATTGTTTTTCTCAG+AGG | + | 50598:4625-4644 | None:intergenic | 40.0% |
| !! | TGATGTCTTTAGCGTTGACT+AGG | + | 50598:4920-4939 | None:intergenic | 40.0% |
| !! | TTCTGAAGCGGTGATGAAAT+TGG | + | 50598:4709-4728 | None:intergenic | 40.0% |
| !!! | AGTTTAGGTTTTTTGGGGGT+GGG | + | 50598:4678-4697 | None:intergenic | 40.0% |
| !!! | CTTTAGCGTTGACTAGGTTT+TGG | + | 50598:4914-4933 | None:intergenic | 40.0% |
| !!! | GTTGGTTTGCCTACTTGTTT+TGG | - | 50598:5725-5744 | MS.gene072599:CDS | 40.0% |
| !!! | TAGTTTAGGTTTTTTGGGGG+TGG | + | 50598:4679-4698 | None:intergenic | 40.0% |
| !!! | TGGTTTGCCTACTTGTTTTG+GGG | - | 50598:5727-5746 | MS.gene072599:CDS | 40.0% |
| AAAGCTTGGGGAACTCATCT+TGG | - | 50598:5668-5687 | MS.gene072599:CDS | 45.0% | |
| AAGCTTGGGGAACTCATCTT+GGG | - | 50598:5669-5688 | MS.gene072599:CDS | 45.0% | |
| AATTGAGCGGGAGTTGTTGA+TGG | + | 50598:4889-4908 | None:intergenic | 45.0% | |
| AGGTTTGAAGGAGGGATTGA+AGG | - | 50598:5593-5612 | MS.gene072599:CDS | 45.0% | |
| ATCTCATCGTTCGAACTCAC+CGG | + | 50598:5143-5162 | None:intergenic | 45.0% | |
| CAAACCCATTATCCACCAGT+TGG | + | 50598:5937-5956 | None:intergenic | 45.0% | |
| CCATACTCATGAGACACAAC+AGG | + | 50598:5485-5504 | None:intergenic | 45.0% | |
| CCTGTTGTGTCTCATGAGTA+TGG | - | 50598:5482-5501 | MS.gene072599:CDS | 45.0% | |
| CGTTCAATCCCAGACATCAA+CGG | - | 50598:4645-4664 | MS.gene072599:CDS | 45.0% | |
| GAACTCACCGGAAAAGGTAA+CGG | + | 50598:5131-5150 | None:intergenic | 45.0% | |
| GGATCAACTTCTCCATCTCT+AGG | + | 50598:5836-5855 | None:intergenic | 45.0% | |
| GGTGTGTTGAAGAACGATAG+CGG | + | 50598:4979-4998 | None:intergenic | 45.0% | |
| GGTTTGAAGGAGGGATTGAA+GGG | - | 50598:5594-5613 | MS.gene072599:CDS | 45.0% | |
| GTTGATGCTGCTCATGCAAT+TGG | - | 50598:5320-5339 | MS.gene072599:CDS | 45.0% | |
| TACAAAAACGCGATCGAAGG+TGG | + | 50598:5419-5438 | None:intergenic | 45.0% | |
| TCAACTTCTCCATCTCTAGG+CGG | + | 50598:5833-5852 | None:intergenic | 45.0% | |
| ! | CGTTGATGTCTGGGATTGAA+CGG | + | 50598:4647-4666 | None:intergenic | 45.0% |
| ! | GAGAGATGTTGGTGAAAGCT+TGG | - | 50598:5654-5673 | MS.gene072599:CDS | 45.0% |
| ! | GAGATGTTGGTGAAAGCTTG+GGG | - | 50598:5656-5675 | MS.gene072599:CDS | 45.0% |
| ! | TGGAGAAGTTGATCCTGTGA+CGG | - | 50598:5841-5860 | MS.gene072599:CDS | 45.0% |
| !! | AAAGTGCTTGGATTGGCACA+AGG | - | 50598:5522-5541 | MS.gene072599:CDS | 45.0% |
| !! | AAGTGCTTGGATTGGCACAA+GGG | - | 50598:5523-5542 | MS.gene072599:CDS | 45.0% |
| !! | CGGTGCTGTTAAGAAATCCA+TGG | - | 50598:5064-5083 | MS.gene072599:CDS | 45.0% |
| !! | GATTAGCTGTGGAAAGTGCT+TGG | - | 50598:5510-5529 | MS.gene072599:CDS | 45.0% |
| !!! | GTTTAGGTTTTTTGGGGGTG+GGG | + | 50598:4677-4696 | None:intergenic | 45.0% |
| ACCTTCACGGAAACACCAAG+CGG | + | 50598:5000-5019 | None:intergenic | 50.0% | |
| CGCATTAACAACGGCAGCTT+TGG | - | 50598:4768-4787 | MS.gene072599:CDS | 50.0% | |
| GGATGCTGTTAACCAACTGG+TGG | - | 50598:5922-5941 | MS.gene072599:CDS | 50.0% | |
| TCCTCTCCGCTCAACAACAA+TGG | - | 50598:4808-4827 | MS.gene072599:CDS | 50.0% | |
| TCGGGATGCTGTTAACCAAC+TGG | - | 50598:5919-5938 | MS.gene072599:CDS | 50.0% | |
| TCGTTCGAACTCACCGGAAA+AGG | + | 50598:5137-5156 | None:intergenic | 50.0% | |
| TCGTTCTTCAACACACCGCT+TGG | - | 50598:4982-5001 | MS.gene072599:CDS | 50.0% | |
| ! | ATTGGTGGTTCCTGAGGCTT+TGG | - | 50598:5559-5578 | MS.gene072599:CDS | 50.0% |
| ! | GAGAAGTTGATCCTGTGACG+GGG | - | 50598:5843-5862 | MS.gene072599:CDS | 50.0% |
| ! | GAGCTGCCATTGTTGTTGAG+CGG | + | 50598:4817-4836 | None:intergenic | 50.0% |
| ! | GGAGAAGTTGATCCTGTGAC+GGG | - | 50598:5842-5861 | MS.gene072599:CDS | 50.0% |
| ! | TGATGTCTGGGATTGAACGG+TGG | + | 50598:4644-4663 | None:intergenic | 50.0% |
| ! | TGCTCAATTGGTGGTTCCTG+AGG | - | 50598:5553-5572 | MS.gene072599:CDS | 50.0% |
| ! | TTGAAGAACGATAGCGGCAG+CGG | + | 50598:4973-4992 | None:intergenic | 50.0% |
| ! | TTGTTGTTGAGCGGAGAGGA+TGG | + | 50598:4808-4827 | None:intergenic | 50.0% |
| !! | AGTTGTTGATGGCGGGTTTG+AGG | + | 50598:4878-4897 | None:intergenic | 50.0% |
| !! | CAGAGAGGAAGGTGTTGAAC+AGG | - | 50598:5292-5311 | MS.gene072599:CDS | 50.0% |
| !! | GCTGTGGAAAGTGCTTGGAT+TGG | - | 50598:5515-5534 | MS.gene072599:CDS | 50.0% |
| ACGAGTGACGTAAGCCTCCA+TGG | + | 50598:5084-5103 | None:intergenic | 55.0% | |
| ATCCTTGCATACCCCGTCAC+AGG | + | 50598:5857-5876 | None:intergenic | 55.0% | |
| GCCGTCGCTCGCATTAACAA+CGG | - | 50598:4759-4778 | MS.gene072599:CDS | 55.0% | |
| GCCGTTGTTAATGCGAGCGA+CGG | + | 50598:4763-4782 | None:intergenic | 55.0% | |
| GTTGTTAATGCGAGCGACGG+CGG | + | 50598:4760-4779 | None:intergenic | 55.0% | |
| TTGTTGAGCGGAGAGGATGG+AGG | + | 50598:4805-4824 | None:intergenic | 55.0% | |
| ! | ATCCTGTGACGGGGTATGCA+AGG | - | 50598:5852-5871 | MS.gene072599:CDS | 55.0% |
| ! | CTTACGTCACTCGTGCTGGT+GGG | - | 50598:5090-5109 | MS.gene072599:CDS | 55.0% |
| ! | GCCATTGTTGTTGAGCGGAG+AGG | + | 50598:4812-4831 | None:intergenic | 55.0% |
| ! | GGTGTGTGCCGTTGATGTCT+GGG | + | 50598:4656-4675 | None:intergenic | 55.0% |
| ! | TGAGCGGGAGTTGTTGATGG+CGG | + | 50598:4886-4905 | None:intergenic | 55.0% |
| !! | ACCGCTTGGTGTTTCCGTGA+AGG | - | 50598:4996-5015 | MS.gene072599:CDS | 55.0% |
| !! | TGTTGATGGCGGGTTTGAGG+TGG | + | 50598:4875-4894 | None:intergenic | 55.0% |
| GAGGCTTACGTCACTCGTGC+TGG | - | 50598:5086-5105 | MS.gene072599:CDS | 60.0% | |
| GCTTACGTCACTCGTGCTGG+TGG | - | 50598:5089-5108 | MS.gene072599:CDS | 60.0% | |
| GGAGAGGATGGAGGAAGGAC+AGG | + | 50598:4796-4815 | None:intergenic | 60.0% | |
| GGCGTCGTGGTGGCTGAATT+CGG | + | 50598:4739-4758 | None:intergenic | 60.0% | |
| TGAGCGGAGAGGATGGAGGA+AGG | + | 50598:4801-4820 | None:intergenic | 60.0% | |
| ! | AAGAACGATAGCGGCAGCGG+TGG | + | 50598:4970-4989 | None:intergenic | 60.0% |
| ! | GGGTGTGTGCCGTTGATGTC+TGG | + | 50598:4657-4676 | None:intergenic | 60.0% |
| !! | GAGCGGGAGTTGTTGATGGC+GGG | + | 50598:4885-4904 | None:intergenic | 60.0% |
| ! | AACGATAGCGGCAGCGGTGG+TGG | + | 50598:4967-4986 | None:intergenic | 65.0% |
| ! | GAGCGACGGCGGCGTCGTGG+TGG | + | 50598:4749-4768 | None:intergenic | 80.0% |
| ! | TGCGAGCGACGGCGGCGTCG+TGG | + | 50598:4752-4771 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 50598 | gene | 4618 | 5979 | 4618 | ID=MS.gene072599 |
| 50598 | mRNA | 4618 | 5979 | 4618 | ID=MS.gene072599.t1;Parent=MS.gene072599 |
| 50598 | exon | 4618 | 5979 | 4618 | ID=MS.gene072599.t1.exon1;Parent=MS.gene072599.t1 |
| 50598 | CDS | 4618 | 5979 | 4618 | ID=cds.MS.gene072599.t1;Parent=MS.gene072599.t1 |
| Gene Sequence |
| Protein sequence |
