AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072743

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072743.t1 MTR_5g009600 96.696 575 19 0 1 575 319 893 0.0 1142
MS.gene072743.t1 MTR_8g027990 51.418 282 113 7 1 277 309 571 4.09e-88 286
MS.gene072743.t1 MTR_1g026160 28.283 198 112 5 17 213 234 402 1.53e-21 97.8
MS.gene072743.t1 MTR_5g023150 28.571 196 110 5 16 210 286 452 1.99e-21 97.8
MS.gene072743.t1 MTR_5g023150 28.571 196 110 5 16 210 383 549 3.23e-21 97.8
MS.gene072743.t1 MTR_2g013900 30.097 206 111 8 22 223 260 436 1.60e-20 95.1
MS.gene072743.t1 MTR_4g088745 28.788 198 101 7 22 213 145 308 1.93e-20 93.6
MS.gene072743.t1 MTR_4g132860 34.896 192 88 7 21 207 178 337 1.99e-20 94.0
MS.gene072743.t1 MTR_4g088755 28.788 198 101 7 22 213 140 303 2.63e-20 93.2
MS.gene072743.t1 MTR_3g114900 29.798 198 109 6 16 212 358 526 4.25e-20 94.4
MS.gene072743.t1 MTR_4g088750 27.619 210 113 6 20 223 299 475 5.73e-20 93.6
MS.gene072743.t1 MTR_7g072710 31.217 189 103 5 21 205 141 306 8.33e-20 91.7
MS.gene072743.t1 MTR_7g072710 31.217 189 103 5 21 205 104 269 9.21e-20 90.9
MS.gene072743.t1 MTR_1g113960 28.502 207 113 6 5 210 383 555 9.41e-20 93.2
MS.gene072743.t1 MTR_1g113960 28.502 207 113 6 5 210 383 555 1.12e-19 93.2
MS.gene072743.t1 MTR_4g105770 30.612 196 108 5 23 218 158 325 1.55e-19 90.9
MS.gene072743.t1 MTR_6g007603 31.088 193 104 7 16 208 186 349 7.19e-19 89.0
MS.gene072743.t1 MTR_3g078000 31.156 199 107 6 22 218 154 324 8.57e-19 88.6
MS.gene072743.t1 MTR_2g461180 34.574 188 97 8 29 213 1234 1398 1.34e-18 90.5
MS.gene072743.t1 MTR_3g078110 31.443 194 102 7 16 208 174 337 1.36e-18 88.2
MS.gene072743.t1 MTR_6g033130 32.632 190 106 4 29 218 1076 1243 1.86e-18 90.1
MS.gene072743.t1 MTR_4g116520 32.821 195 101 6 12 206 1192 1356 2.01e-18 90.1
MS.gene072743.t1 MTR_4g106980 31.088 193 104 7 16 208 203 366 2.70e-18 87.4
MS.gene072743.t1 MTR_8g442400 31.492 181 100 5 29 208 1495 1652 4.34e-18 89.0
MS.gene072743.t1 MTR_1g099110 27.273 198 108 6 5 201 348 510 7.03e-18 87.4
MS.gene072743.t1 MTR_1g099110 27.273 198 108 6 5 201 365 527 9.33e-18 87.0
MS.gene072743.t1 MTR_4g128650 33.146 178 97 4 29 206 920 1075 3.28e-17 86.3
MS.gene072743.t1 MTR_1g050520 28.696 230 115 9 8 208 106 315 3.47e-16 82.4
MS.gene072743.t1 MTR_4g082325 29.596 223 122 10 4 221 225 417 9.98e-16 80.1
MS.gene072743.t1 MTR_3g076970 27.895 190 99 5 25 205 126 286 1.60e-15 78.6
MS.gene072743.t1 MTR_1g090020 30.244 205 115 6 14 212 988 1170 2.34e-15 80.1
MS.gene072743.t1 MTR_5g006560 27.461 193 111 6 16 208 201 364 5.75e-15 77.4
MS.gene072743.t1 MTR_5g006560 27.717 184 106 5 25 208 242 398 1.52e-14 76.3
MS.gene072743.t1 MTR_5g019700 30.481 187 108 7 19 205 137 301 1.76e-14 76.3
MS.gene072743.t1 MTR_4g122160 34.254 181 76 9 27 198 559 705 2.21e-14 76.6
MS.gene072743.t1 MTR_2g034020 30.570 193 91 9 27 210 565 723 1.01e-13 74.7
MS.gene072743.t1 MTR_8g107000 29.101 189 95 7 29 210 830 986 1.31e-12 71.2
MS.gene072743.t1 MTR_1g026160 27.941 136 71 3 17 152 234 342 1.49e-12 69.7
MS.gene072743.t1 MTR_1g026160 27.941 136 71 3 17 152 234 342 1.53e-12 69.7
MS.gene072743.t1 MTR_1g026160 27.941 136 71 3 17 152 379 487 1.65e-12 70.5
MS.gene072743.t1 MTR_1g026160 27.941 136 71 3 17 152 379 487 1.67e-12 70.5
MS.gene072743.t1 MTR_1g026160 27.941 136 71 3 17 152 232 340 1.70e-12 69.7
MS.gene072743.t1 MTR_1g026160 27.941 136 71 3 17 152 232 340 1.76e-12 69.3
MS.gene072743.t1 MTR_3g465590 28.177 181 105 5 26 205 424 580 2.36e-12 70.1
MS.gene072743.t1 MTR_5g066490 30.481 187 95 8 29 210 749 905 3.14e-12 70.1
MS.gene072743.t1 MTR_1g081790 27.083 192 103 7 27 212 788 948 5.21e-12 69.3
MS.gene072743.t1 MTR_6g079540 29.255 188 96 7 29 210 761 917 7.67e-12 68.9
MS.gene072743.t1 MTR_6g079540 29.255 188 96 7 29 210 513 669 9.98e-12 68.2
MS.gene072743.t1 MTR_8g088590 30.526 190 103 7 26 208 272 439 1.05e-11 67.8
MS.gene072743.t1 MTR_1g061190 26.882 186 107 5 27 210 656 814 6.01e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072743.t1 AT1G64300 44.444 459 191 12 1 449 311 715 1.50e-112 352
MS.gene072743.t1 AT1G64300 44.444 459 191 12 1 449 311 715 1.50e-112 352
MS.gene072743.t1 AT5G41730 44.601 426 181 10 1 408 311 699 2.02e-108 341
MS.gene072743.t1 AT5G41730 44.601 426 181 10 1 408 311 699 2.02e-108 341
MS.gene072743.t1 AT1G01450 52.482 282 113 6 1 276 101 367 1.32e-91 290
MS.gene072743.t1 AT3G46930 29.245 212 117 5 22 227 297 481 2.19e-23 104
MS.gene072743.t1 AT3G46930 29.245 212 117 5 22 227 262 446 4.70e-23 103
MS.gene072743.t1 AT5G01850 30.526 190 105 5 20 205 113 279 3.77e-20 92.4
MS.gene072743.t1 AT1G62400 33.862 189 89 6 21 205 184 340 3.86e-20 93.2
MS.gene072743.t1 AT5G01850 30.526 190 105 5 20 205 137 303 6.16e-20 92.0
MS.gene072743.t1 AT5G58950 29.101 189 98 7 22 205 306 463 2.11e-19 92.0
MS.gene072743.t1 AT2G35050 32.778 180 95 7 29 206 1082 1237 1.12e-18 90.9
MS.gene072743.t1 AT2G35050 32.778 180 95 7 29 206 1082 1237 1.12e-18 90.9
MS.gene072743.t1 AT4G38470 27.143 210 118 6 5 213 372 547 2.08e-18 89.4
MS.gene072743.t1 AT5G50000 30.570 193 105 6 16 208 196 359 3.04e-18 87.4
MS.gene072743.t1 AT2G40860 30.964 197 98 6 22 196 24 204 4.91e-18 88.2
MS.gene072743.t1 AT2G40860 30.964 197 98 6 22 196 122 302 5.49e-18 88.2
MS.gene072743.t1 AT2G40860 30.964 197 98 6 22 196 122 302 5.70e-18 88.2
MS.gene072743.t1 AT2G40860 30.964 197 98 6 22 196 122 302 7.70e-18 87.4
MS.gene072743.t1 AT2G17700 28.061 196 111 7 16 210 374 540 7.74e-18 87.4
MS.gene072743.t1 AT4G38470 26.829 205 115 6 5 208 372 542 7.74e-18 87.4
MS.gene072743.t1 AT4G35780 27.273 187 106 5 20 205 384 541 1.01e-17 87.0
MS.gene072743.t1 AT5G57610 30.645 186 107 4 29 214 890 1053 1.04e-17 87.8
MS.gene072743.t1 AT1G04700 32.584 178 98 4 29 206 875 1030 1.08e-17 87.8
MS.gene072743.t1 AT1G04700 32.584 178 98 4 29 206 875 1030 1.08e-17 87.8
MS.gene072743.t1 AT3G01490 31.443 194 102 8 16 208 222 385 1.08e-17 85.9
MS.gene072743.t1 AT3G46920 31.461 178 100 4 29 206 981 1136 2.33e-17 86.7
MS.gene072743.t1 AT3G46920 31.461 178 100 4 29 206 915 1070 2.33e-17 86.7
MS.gene072743.t1 AT1G16270 29.787 188 108 5 29 215 972 1136 3.23e-17 86.3
MS.gene072743.t1 AT1G16270 29.787 188 108 5 29 215 972 1136 3.23e-17 86.3
MS.gene072743.t1 AT1G16270 29.787 188 108 5 29 215 972 1136 3.23e-17 86.3
MS.gene072743.t1 AT3G24715 32.203 177 97 5 29 204 945 1099 4.93e-17 85.5
MS.gene072743.t1 AT3G24715 32.203 177 97 5 29 204 945 1099 4.93e-17 85.5
MS.gene072743.t1 AT3G24715 32.203 177 97 5 29 204 945 1099 4.93e-17 85.5
MS.gene072743.t1 AT1G14000 31.982 222 118 11 4 221 244 436 6.61e-17 84.0
MS.gene072743.t1 AT1G79570 30.000 180 104 4 29 208 1073 1230 7.16e-17 85.1
MS.gene072743.t1 AT1G79570 30.000 180 104 4 29 208 1073 1230 7.16e-17 85.1
MS.gene072743.t1 AT4G31170 28.095 210 114 8 5 205 204 385 1.26e-16 82.8
MS.gene072743.t1 AT4G31170 28.095 210 114 8 5 205 204 385 1.26e-16 82.8
MS.gene072743.t1 AT4G31170 28.095 210 114 8 5 205 204 385 1.26e-16 82.8
MS.gene072743.t1 AT4G31170 28.095 210 114 8 5 205 204 385 1.26e-16 82.8
MS.gene072743.t1 AT4G31170 28.095 210 114 8 5 205 204 385 1.26e-16 82.8
MS.gene072743.t1 AT4G31170 28.095 210 114 8 5 205 204 385 1.26e-16 82.8
MS.gene072743.t1 AT4G31170 28.095 210 114 8 5 205 204 385 1.26e-16 82.8
MS.gene072743.t1 AT3G22750 26.961 204 111 6 14 208 178 352 1.59e-16 82.0
MS.gene072743.t1 AT5G40540 28.723 188 108 7 21 205 25 189 1.97e-16 79.7
MS.gene072743.t1 AT3G27560 28.788 198 111 8 15 208 119 290 2.77e-16 81.3
MS.gene072743.t1 AT5G50180 29.891 184 103 6 25 205 120 280 2.96e-16 80.9
MS.gene072743.t1 AT5G40540 28.723 188 108 7 21 205 46 210 3.83e-16 79.3
MS.gene072743.t1 AT5G66710 28.934 197 106 6 27 217 174 342 7.25e-16 80.5
MS.gene072743.t1 AT3G63260 28.649 185 101 5 26 208 210 365 7.67e-16 80.1
MS.gene072743.t1 AT5G40540 28.723 188 108 7 21 205 122 286 1.04e-15 79.3
MS.gene072743.t1 AT2G24360 27.322 183 105 5 23 205 230 384 1.89e-15 79.0
MS.gene072743.t1 AT3G50730 27.094 203 119 7 5 205 121 296 2.10e-15 78.6
MS.gene072743.t1 AT3G06630 32.632 190 91 9 27 210 533 691 3.27e-15 79.3
MS.gene072743.t1 AT4G14780 26.316 190 109 5 21 208 178 338 8.10e-15 76.6
MS.gene072743.t1 AT5G49470 31.053 190 94 9 27 210 564 722 1.24e-14 77.8
MS.gene072743.t1 AT5G49470 31.053 190 94 9 27 210 590 748 1.38e-14 77.4
MS.gene072743.t1 AT5G49470 31.053 190 94 9 27 210 463 621 1.66e-14 77.0
MS.gene072743.t1 AT5G49470 30.570 193 91 9 27 210 303 461 1.75e-14 76.6
MS.gene072743.t1 AT5G49470 30.412 194 98 9 23 210 647 809 2.91e-14 76.6
MS.gene072743.t1 AT4G24480 30.851 188 93 8 29 210 777 933 1.06e-13 74.7
MS.gene072743.t1 AT4G24480 30.851 188 93 8 29 210 772 928 1.09e-13 74.7
MS.gene072743.t1 AT4G24480 30.851 188 93 8 29 210 772 928 1.21e-13 74.7
MS.gene072743.t1 AT3G46930 29.412 136 67 4 22 155 297 405 1.51e-13 73.6
MS.gene072743.t1 AT3G46930 29.412 136 67 4 22 155 297 405 1.56e-13 73.2
MS.gene072743.t1 AT3G46930 29.412 136 67 4 22 155 201 309 2.10e-13 72.0
MS.gene072743.t1 AT3G46930 29.412 136 67 4 22 155 262 370 2.43e-13 72.4
MS.gene072743.t1 AT3G50720 29.381 194 114 7 21 213 145 316 6.36e-13 71.2
MS.gene072743.t1 AT2G42290 30.964 197 108 6 34 209 457 646 7.28e-13 72.0
MS.gene072743.t1 AT1G18160 30.270 185 98 7 29 210 816 972 1.67e-12 71.2
MS.gene072743.t1 AT3G57830 29.146 199 111 6 34 209 469 660 3.14e-12 69.7
MS.gene072743.t1 AT3G57830 29.146 199 111 6 34 209 469 660 3.14e-12 69.7
MS.gene072743.t1 AT1G73660 30.270 185 98 7 29 210 849 1005 5.03e-12 69.7
MS.gene072743.t1 AT1G08720 27.083 192 103 7 27 212 768 928 5.57e-12 69.3
MS.gene072743.t1 AT5G03730 26.601 203 112 6 10 210 643 810 1.37e-11 68.2
MS.gene072743.t1 AT5G03730 26.601 203 112 6 10 210 643 810 1.37e-11 68.2

Find 104 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCTCCTGCTTTGATATTT+TGG 0.080066 5.3:+3519592 None:intergenic
CTCTCAGATTCTGAGACTTT+TGG 0.146039 5.3:+3518752 None:intergenic
ATCAAACTCACAAGATATTT+TGG 0.164131 5.3:+3519546 None:intergenic
TTGATTCCCTTTGTTAATAT+TGG 0.196770 5.3:+3518724 None:intergenic
CAAGCAAAAGTTGTTGGATT+TGG 0.204645 5.3:-3519873 MS.gene072743:CDS
GCTTATAAAGTTGTTGAAAA+AGG 0.240086 5.3:-3519273 MS.gene072743:CDS
GTGTCATCAGAAATTGATTT+TGG 0.241755 5.3:+3519078 None:intergenic
GTAGGATATTACAAGGATTA+AGG 0.243595 5.3:+3519928 None:intergenic
TATTCCTACTATAGATAATA+AGG 0.252638 5.3:-3519217 MS.gene072743:CDS
AAGAGATTAACCCTATAATT+TGG 0.257480 5.3:-3519779 MS.gene072743:CDS
AACTGACCTTGGTCTTTCTT+TGG 0.265277 5.3:+3518988 None:intergenic
GGTTACCAGATCTTTAATTA+TGG 0.266121 5.3:+3519104 None:intergenic
TCAGGTGCATACCAAATTAT+AGG 0.292831 5.3:+3519768 None:intergenic
CTGGTTTCTCATCTATTTGT+AGG 0.294229 5.3:-3519488 MS.gene072743:CDS
TTTGTCTTTAACCTGTTAAA+TGG 0.297516 5.3:+3518599 None:intergenic
TATGGTCATGTCTCAGATTA+AGG 0.304997 5.3:-3518272 None:intergenic
TGAAACATGGCAGCATCTTC+TGG 0.314429 5.3:+3518442 None:intergenic
GGATTGTCATCTGTTGAAAA+TGG 0.333696 5.3:-3519852 MS.gene072743:CDS
GTCCTTTGATTGCTCACATA+TGG 0.339488 5.3:+3518356 None:intergenic
AGCATTGTGAGGATGCAAAA+AGG 0.341134 5.3:-3518506 MS.gene072743:CDS
CATTATCTGGTCGGGTTTCA+AGG 0.346406 5.3:-3518838 MS.gene072743:CDS
TTGACACGTCTTATTCTTGT+TGG 0.355333 5.3:+3518542 None:intergenic
TCGGGTTTCAAGGACAAATA+AGG 0.364030 5.3:-3518828 MS.gene072743:CDS
GAGACCAATCACCATTTAAC+AGG 0.371640 5.3:-3518610 MS.gene072743:CDS
TTCTTTGGCTTCATTTGTGA+TGG 0.379395 5.3:+3519003 None:intergenic
TTAACCTGTTAAATGGTGAT+TGG 0.382051 5.3:+3518606 None:intergenic
AGTTCAAGCTGATTTAGTTC+AGG 0.389760 5.3:+3519408 None:intergenic
ATCTCATGCTTCAAATGGCC+AGG 0.399868 5.3:-3519992 MS.gene072743:CDS
ATTGTAACATTCTTCTTTGA+CGG 0.401100 5.3:+3519033 None:intergenic
GGGTTAATCTCTTCACCAAT+TGG 0.403263 5.3:+3519789 None:intergenic
CCATATGTTGTTCTTACATA+AGG 0.409101 5.3:+3518290 None:intergenic
ACAGTAGCCACATCCTTTGT+TGG 0.413590 5.3:+3519180 None:intergenic
TCATTACCACCAACATTATC+TGG 0.424357 5.3:-3518851 MS.gene072743:CDS
ATGCTGCCATGTTTCAGATA+TGG 0.424537 5.3:-3518435 MS.gene072743:CDS
TTATAAGCAAACATTTCATA+AGG 0.434328 5.3:+3519288 None:intergenic
CATCTTCACAAACTGTGCTT+TGG 0.436780 5.3:+3519136 None:intergenic
TGCATCCATAATTAAAGATC+TGG 0.439192 5.3:-3519109 MS.gene072743:CDS
TTGTAACATTCTTCTTTGAC+GGG 0.441892 5.3:+3519034 None:intergenic
CTCAAAGGGCTCAATGCTAA+TGG 0.444928 5.3:+3518314 None:intergenic
CTCAAAGGACTCAATGGAAA+TGG 0.446507 5.3:+3518380 None:intergenic
ATTCCTACTATAGATAATAA+GGG 0.450157 5.3:-3519216 MS.gene072743:CDS
TGAAAATGATTATCCTCAAA+AGG 0.452553 5.3:+3519630 None:intergenic
GAGAACAAAATCTGTCTCCT+TGG 0.464084 5.3:+3520032 None:intergenic
ACCACCAACATTATCTGGTC+GGG 0.478543 5.3:-3518846 MS.gene072743:CDS
CACCGCCAATGAGTCCTTCA+AGG 0.481111 5.3:-3518472 MS.gene072743:CDS
AAAGCCGATGCATATAGCTT+TGG 0.483467 5.3:-3519687 MS.gene072743:CDS
CAGGTGCATACCAAATTATA+GGG 0.486379 5.3:+3519769 None:intergenic
TCACCAACACATAATCCAAT+TGG 0.489553 5.3:-3519804 MS.gene072743:CDS
TACAAGGATTAAGGTTTCCA+TGG 0.498240 5.3:+3519937 None:intergenic
TATGGACTCAAAGGACTCAA+TGG 0.498751 5.3:+3518374 None:intergenic
CTACCCTTATTATCTATAGT+AGG 0.503856 5.3:+3519213 None:intergenic
ACCAACCAAAATATCAAAGC+AGG 0.507591 5.3:-3519597 MS.gene072743:CDS
GTTCTTACATAAGGACTCAA+AGG 0.508350 5.3:+3518299 None:intergenic
TCTAGCTCAGTTAAAACCTC+AGG 0.514317 5.3:+3519750 None:intergenic
ACTGATCCGTCTCAGCGTCC+TGG 0.518950 5.3:-3519507 MS.gene072743:CDS
CTTCTGGTGTATGACCTTGA+AGG 0.519264 5.3:+3518458 None:intergenic
AGTTGATGAACAAGGATTTG+TGG 0.524686 5.3:-3520079 None:intergenic
GGAAATCCAATATTAACAAA+GGG 0.528711 5.3:-3518730 MS.gene072743:CDS
CCTTATGTAAGAACAACATA+TGG 0.529072 5.3:-3518290 MS.gene072743:CDS
CTATAACCCCAGTTAAAAGA+AGG 0.532741 5.3:-3518778 MS.gene072743:CDS
AGGAAATCCAATATTAACAA+AGG 0.532942 5.3:-3518731 MS.gene072743:CDS
TATGACCTTGAAGGACTCAT+TGG 0.538589 5.3:+3518467 None:intergenic
TTTGTTCTCTATACCTGTGG+TGG 0.547282 5.3:-3520018 MS.gene072743:CDS
GTAGATTGTTGTGACATTGA+AGG 0.548073 5.3:-3519372 MS.gene072743:CDS
ATATTATATGTCCTCTCCAT+AGG 0.548736 5.3:+3519330 None:intergenic
TATAATTTGGTATGCACCTG+AGG 0.550991 5.3:-3519766 MS.gene072743:CDS
TTCTTACATAAGGACTCAAA+GGG 0.551714 5.3:+3518300 None:intergenic
GGTTGATCTCATGCTTCAAA+TGG 0.555778 5.3:-3519997 MS.gene072743:CDS
AGCAATCAAAGGACTAGAAG+AGG 0.562315 5.3:-3518347 MS.gene072743:CDS
GGCGAGCGATATGGAAACAA+AGG 0.564210 5.3:+3519567 None:intergenic
GTGGAGATATTCAATGCCCC+TGG 0.567677 5.3:+3519974 None:intergenic
TCAATGTCACAACAATCTAC+AGG 0.568956 5.3:+3519375 None:intergenic
GACCTTGAAGGACTCATTGG+CGG 0.570717 5.3:+3518470 None:intergenic
TCACCAATTGGATTATGTGT+TGG 0.571772 5.3:+3519801 None:intergenic
GAGAAACCAGGACGCTGAGA+CGG 0.572704 5.3:+3519501 None:intergenic
TACCACCAACATTATCTGGT+CGG 0.572730 5.3:-3518847 MS.gene072743:CDS
GTCTGAGCCAACAAAGGATG+TGG 0.574953 5.3:-3519187 MS.gene072743:CDS
TTGCTCACATATGGACTCAA+AGG 0.582014 5.3:+3518365 None:intergenic
TCTCATGCTTCAAATGGCCA+GGG 0.583448 5.3:-3519991 MS.gene072743:CDS
GTCCATATGTGAGCAATCAA+AGG 0.596259 5.3:-3518358 MS.gene072743:CDS
CATACCAAAGCTATATGCAT+CGG 0.599646 5.3:+3519683 None:intergenic
AAAGCTAGAAACTCACAAGA+AGG 0.600879 5.3:-3519903 MS.gene072743:CDS
AACTTGATGTCAATGATGAC+TGG 0.601724 5.3:+3518801 None:intergenic
TGAGAGAGAATTGTGGTCCA+AGG 0.604253 5.3:-3520049 MS.gene072743:CDS
TTTCTCTGAGTACTTACAAG+AGG 0.613541 5.3:+3519707 None:intergenic
ACCCGACCAGATAATGTTGG+TGG 0.618092 5.3:+3518845 None:intergenic
AGCATGAGATCAACCACCAC+AGG 0.618436 5.3:+3520005 None:intergenic
AAAATATCAAAGCAGGAGAG+AGG 0.620693 5.3:-3519590 MS.gene072743:CDS
GAAACCCGACCAGATAATGT+TGG 0.622199 5.3:+3518842 None:intergenic
CTACAAATAGATGAGAAACC+AGG 0.623694 5.3:+3519489 None:intergenic
AACTATCCATATCTGAAACA+TGG 0.625924 5.3:+3518429 None:intergenic
ATGTCACAACAATCTACAGG+TGG 0.639302 5.3:+3519378 None:intergenic
AGGATTGATCAAAACATACT+CGG 0.640765 5.3:+3519428 None:intergenic
CAAAAGTCTCAGAATCTGAG+AGG 0.650694 5.3:-3518751 MS.gene072743:CDS
TAGTGAGTCTGAGCCAACAA+AGG 0.653550 5.3:-3519193 MS.gene072743:CDS
AATTGGATTATGTGTTGGTG+AGG 0.655784 5.3:+3519806 None:intergenic
TGTGACGGAGTTGATGAACA+AGG 0.656381 5.3:-3520087 None:intergenic
CTCATGCTTCAAATGGCCAG+GGG 0.668271 5.3:-3519990 MS.gene072743:CDS
ATGAAGCCAAAGAAAGACCA+AGG 0.681190 5.3:-3518994 MS.gene072743:intron
TGTAACATTCTTCTTTGACG+GGG 0.687811 5.3:+3519035 None:intergenic
GTACTAACAACAGCATTGTG+AGG 0.690491 5.3:-3518517 MS.gene072743:CDS
GATAGTTCTTTGAAGATACG+AGG 0.692687 5.3:-3518413 MS.gene072743:CDS
TGTCACAACAATCTACAGGT+GGG 0.697110 5.3:+3519379 None:intergenic
ACATACATGAGAGAGAATTG+TGG 0.732943 5.3:-3520056 MS.gene072743:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATATATATATATAGC+AGG + chr5.3:3519413-3519432 None:intergenic 10.0%
!! ATTCCTACTATAGATAATAA+GGG - chr5.3:3519132-3519151 MS.gene072743:CDS 20.0%
!! CTTATAAAGTTGTTGAAAAA+GGG - chr5.3:3519076-3519095 MS.gene072743:CDS 20.0%
!! TATTCCTACTATAGATAATA+AGG - chr5.3:3519131-3519150 MS.gene072743:CDS 20.0%
!! TTATAAGCAAACATTTCATA+AGG + chr5.3:3519063-3519082 None:intergenic 20.0%
!!! TCAGTTAGAAAAAAAGTATA+TGG - chr5.3:3519671-3519690 MS.gene072743:CDS 20.0%
! AAGAGATTAACCCTATAATT+TGG - chr5.3:3518569-3518588 MS.gene072743:CDS 25.0%
! AGGAAATCCAATATTAACAA+AGG - chr5.3:3519617-3519636 MS.gene072743:CDS 25.0%
! ATTGTAACATTCTTCTTTGA+CGG + chr5.3:3519318-3519337 None:intergenic 25.0%
! CAATAAAATTAAACTGACCT+TGG + chr5.3:3519374-3519393 None:intergenic 25.0%
! GCTTATAAAGTTGTTGAAAA+AGG - chr5.3:3519075-3519094 MS.gene072743:CDS 25.0%
! GGAAATCCAATATTAACAAA+GGG - chr5.3:3519618-3519637 MS.gene072743:CDS 25.0%
! TGAAAATGATTATCCTCAAA+AGG + chr5.3:3518721-3518740 None:intergenic 25.0%
! TGAGTTTGATCAAAAAATGT+TGG - chr5.3:3518815-3518834 MS.gene072743:CDS 25.0%
! TGTATTGAATTGATTGAAAC+AGG - chr5.3:3519449-3519468 MS.gene072743:CDS 25.0%
! TTGATTCCCTTTGTTAATAT+TGG + chr5.3:3519627-3519646 None:intergenic 25.0%
! TTTGTCTTTAACCTGTTAAA+TGG + chr5.3:3519752-3519771 None:intergenic 25.0%
!! ATCAAACTCACAAGATATTT+TGG + chr5.3:3518805-3518824 None:intergenic 25.0%
!! GTTAGAAAAAAAGTATATGG+AGG - chr5.3:3519674-3519693 MS.gene072743:CDS 25.0%
!! TTGAGGATAATCATTTTCAA+GGG - chr5.3:3518722-3518741 MS.gene072743:CDS 25.0%
!! TTTGAGGATAATCATTTTCA+AGG - chr5.3:3518721-3518740 MS.gene072743:CDS 25.0%
!!! ATCAGAAATTGATTTTGGAT+AGG + chr5.3:3519268-3519287 None:intergenic 25.0%
!!! CTTGTTTTCAGATTTAAACT+AGG + chr5.3:3519719-3519738 None:intergenic 25.0%
!!! TATTTTCAAGCAAAAGTTGT+TGG - chr5.3:3518469-3518488 MS.gene072743:CDS 25.0%
AGGATTGATCAAAACATACT+CGG + chr5.3:3518923-3518942 None:intergenic 30.0%
ATATTATATGTCCTCTCCAT+AGG + chr5.3:3519021-3519040 None:intergenic 30.0%
CCATATGTTGTTCTTACATA+AGG + chr5.3:3520061-3520080 None:intergenic 30.0%
CCTTATGTAAGAACAACATA+TGG - chr5.3:3520058-3520077 MS.gene072743:CDS 30.0%
CTACCCTTATTATCTATAGT+AGG + chr5.3:3519138-3519157 None:intergenic 30.0%
GGTTACCAGATCTTTAATTA+TGG + chr5.3:3519247-3519266 None:intergenic 30.0%
GTAGGATATTACAAGGATTA+AGG + chr5.3:3518423-3518442 None:intergenic 30.0%
TGCATCCATAATTAAAGATC+TGG - chr5.3:3519239-3519258 MS.gene072743:CDS 30.0%
TTAACCTGTTAAATGGTGAT+TGG + chr5.3:3519745-3519764 None:intergenic 30.0%
TTCTTACATAAGGACTCAAA+GGG + chr5.3:3520051-3520070 None:intergenic 30.0%
TTGTAACATTCTTCTTTGAC+GGG + chr5.3:3519317-3519336 None:intergenic 30.0%
! AACTATCCATATCTGAAACA+TGG + chr5.3:3519922-3519941 None:intergenic 30.0%
! GCTTTTAGTAGGATATTACA+AGG + chr5.3:3518430-3518449 None:intergenic 30.0%
! GTTTCCATGGTAAATGTTTT+TGG + chr5.3:3518401-3518420 None:intergenic 30.0%
! TTTTCTCAAGAGTTTTCCTA+TGG - chr5.3:3519002-3519021 MS.gene072743:CDS 30.0%
!! GATTTTGTTGCTTGCAATTT+AGG + chr5.3:3519479-3519498 None:intergenic 30.0%
!! GTGTCATCAGAAATTGATTT+TGG + chr5.3:3519273-3519292 None:intergenic 30.0%
!!! ATATGTTTTGAGTTGTTGAC+AGG - chr5.3:3518688-3518707 MS.gene072743:CDS 30.0%
!!! TATGTTTTGAGTTGTTGACA+GGG - chr5.3:3518689-3518708 MS.gene072743:CDS 30.0%
AAAATATCAAAGCAGGAGAG+AGG - chr5.3:3518758-3518777 MS.gene072743:CDS 35.0%
AAAGCTAGAAACTCACAAGA+AGG - chr5.3:3518445-3518464 MS.gene072743:CDS 35.0%
AACTTGATGTCAATGATGAC+TGG + chr5.3:3519550-3519569 None:intergenic 35.0%
ACATACATGAGAGAGAATTG+TGG - chr5.3:3518292-3518311 MS.gene072743:CDS 35.0%
ACCAACCAAAATATCAAAGC+AGG - chr5.3:3518751-3518770 MS.gene072743:CDS 35.0%
CAAGCAAAAGTTGTTGGATT+TGG - chr5.3:3518475-3518494 MS.gene072743:CDS 35.0%
CACTCCAAAAACATTTACCA+TGG - chr5.3:3518394-3518413 MS.gene072743:CDS 35.0%
CAGGTGCATACCAAATTATA+GGG + chr5.3:3518582-3518601 None:intergenic 35.0%
CATACCAAAGCTATATGCAT+CGG + chr5.3:3518668-3518687 None:intergenic 35.0%
CTACAAATAGATGAGAAACC+AGG + chr5.3:3518862-3518881 None:intergenic 35.0%
CTATAACCCCAGTTAAAAGA+AGG - chr5.3:3519570-3519589 MS.gene072743:CDS 35.0%
CTGGTTTCTCATCTATTTGT+AGG - chr5.3:3518860-3518879 MS.gene072743:CDS 35.0%
GGATTGTCATCTGTTGAAAA+TGG - chr5.3:3518496-3518515 MS.gene072743:CDS 35.0%
GTAGATTGTTGTGACATTGA+AGG - chr5.3:3518976-3518995 MS.gene072743:intron 35.0%
GTTCTTACATAAGGACTCAA+AGG + chr5.3:3520052-3520071 None:intergenic 35.0%
TACAAGGATTAAGGTTTCCA+TGG + chr5.3:3518414-3518433 None:intergenic 35.0%
TATAATTTGGTATGCACCTG+AGG - chr5.3:3518582-3518601 MS.gene072743:CDS 35.0%
TCAATGTCACAACAATCTAC+AGG + chr5.3:3518976-3518995 None:intergenic 35.0%
TCACCAACACATAATCCAAT+TGG - chr5.3:3518544-3518563 MS.gene072743:CDS 35.0%
TCACCAATTGGATTATGTGT+TGG + chr5.3:3518550-3518569 None:intergenic 35.0%
TCAGGTGCATACCAAATTAT+AGG + chr5.3:3518583-3518602 None:intergenic 35.0%
TCATTACCACCAACATTATC+TGG - chr5.3:3519497-3519516 MS.gene072743:CDS 35.0%
TGTAACATTCTTCTTTGACG+GGG + chr5.3:3519316-3519335 None:intergenic 35.0%
TTTCTCTGAGTACTTACAAG+AGG + chr5.3:3518644-3518663 None:intergenic 35.0%
! AATTGGATTATGTGTTGGTG+AGG + chr5.3:3518545-3518564 None:intergenic 35.0%
! AGTTCAAGCTGATTTAGTTC+AGG + chr5.3:3518943-3518962 None:intergenic 35.0%
! GATAGTTCTTTGAAGATACG+AGG - chr5.3:3519935-3519954 MS.gene072743:CDS 35.0%
! GATTTTGTTCTCTATACCTG+TGG - chr5.3:3518327-3518346 MS.gene072743:CDS 35.0%
! TCTCTCCTGCTTTGATATTT+TGG + chr5.3:3518759-3518778 None:intergenic 35.0%
! TTCTTTGGCTTCATTTGTGA+TGG + chr5.3:3519348-3519367 None:intergenic 35.0%
! TTGACACGTCTTATTCTTGT+TGG + chr5.3:3519809-3519828 None:intergenic 35.0%
!!! CATGGTAAATGTTTTTGGAG+TGG + chr5.3:3518396-3518415 None:intergenic 35.0%
!!! GTGAGTTTCTAGCTTTTAGT+AGG + chr5.3:3518441-3518460 None:intergenic 35.0%
!!! TCCTGCTTTGATATTTTGGT+TGG + chr5.3:3518755-3518774 None:intergenic 35.0%
AAAGCCGATGCATATAGCTT+TGG - chr5.3:3518661-3518680 MS.gene072743:CDS 40.0%
AGCAATCAAAGGACTAGAAG+AGG - chr5.3:3520001-3520020 MS.gene072743:CDS 40.0%
AGCATTGTGAGGATGCAAAA+AGG - chr5.3:3519842-3519861 MS.gene072743:CDS 40.0%
ATGAAGCCAAAGAAAGACCA+AGG - chr5.3:3519354-3519373 MS.gene072743:CDS 40.0%
ATGCTGCCATGTTTCAGATA+TGG - chr5.3:3519913-3519932 MS.gene072743:CDS 40.0%
ATGTCACAACAATCTACAGG+TGG + chr5.3:3518973-3518992 None:intergenic 40.0%
CAAAAGTCTCAGAATCTGAG+AGG - chr5.3:3519597-3519616 MS.gene072743:CDS 40.0%
CATCTTCACAAACTGTGCTT+TGG + chr5.3:3519215-3519234 None:intergenic 40.0%
CTCAAAGGACTCAATGGAAA+TGG + chr5.3:3519971-3519990 None:intergenic 40.0%
GAGAACAAAATCTGTCTCCT+TGG + chr5.3:3518319-3518338 None:intergenic 40.0%
GAGACCAATCACCATTTAAC+AGG - chr5.3:3519738-3519757 MS.gene072743:CDS 40.0%
GCATTGTGAGGATGCAAAAA+GGG - chr5.3:3519843-3519862 MS.gene072743:CDS 40.0%
GGGTTAATCTCTTCACCAAT+TGG + chr5.3:3518562-3518581 None:intergenic 40.0%
GGTTGATCTCATGCTTCAAA+TGG - chr5.3:3518351-3518370 MS.gene072743:CDS 40.0%
GTACTAACAACAGCATTGTG+AGG - chr5.3:3519831-3519850 MS.gene072743:CDS 40.0%
GTCCATATGTGAGCAATCAA+AGG - chr5.3:3519990-3520009 MS.gene072743:CDS 40.0%
GTCCTTTGATTGCTCACATA+TGG + chr5.3:3519995-3520014 None:intergenic 40.0%
TACCACCAACATTATCTGGT+CGG - chr5.3:3519501-3519520 MS.gene072743:CDS 40.0%
TATGACCTTGAAGGACTCAT+TGG + chr5.3:3519884-3519903 None:intergenic 40.0%
TATGGACTCAAAGGACTCAA+TGG + chr5.3:3519977-3519996 None:intergenic 40.0%
TCGGGTTTCAAGGACAAATA+AGG - chr5.3:3519520-3519539 MS.gene072743:CDS 40.0%
TGTCACAACAATCTACAGGT+GGG + chr5.3:3518972-3518991 None:intergenic 40.0%
TTGCTCACATATGGACTCAA+AGG + chr5.3:3519986-3520005 None:intergenic 40.0%
TTTGTTCTCTATACCTGTGG+TGG - chr5.3:3518330-3518349 MS.gene072743:CDS 40.0%
! AACTGACCTTGGTCTTTCTT+TGG + chr5.3:3519363-3519382 None:intergenic 40.0%
! GACAGGGAAAATTCCTTTTG+AGG - chr5.3:3518705-3518724 MS.gene072743:CDS 40.0%
! TCAAGAGTTTTCCTATGGAG+AGG - chr5.3:3519007-3519026 MS.gene072743:CDS 40.0%
! TCTAGCTCAGTTAAAACCTC+AGG + chr5.3:3518601-3518620 None:intergenic 40.0%
! TTTGGAGGCCTTCTTTTAAC+TGG + chr5.3:3519581-3519600 None:intergenic 40.0%
!! TTGGAGGCCTTCTTTTAACT+GGG + chr5.3:3519580-3519599 None:intergenic 40.0%
!!! AGATATTTTGGCGAGCGATA+TGG + chr5.3:3518793-3518812 None:intergenic 40.0%
!!! CTCTCAGATTCTGAGACTTT+TGG + chr5.3:3519599-3519618 None:intergenic 40.0%
!!! TCAGATTCTGAGACTTTTGG+AGG + chr5.3:3519596-3519615 None:intergenic 40.0%
ACAGTAGCCACATCCTTTGT+TGG + chr5.3:3519171-3519190 None:intergenic 45.0%
ACCACCAACATTATCTGGTC+GGG - chr5.3:3519502-3519521 MS.gene072743:CDS 45.0%
ATCTCATGCTTCAAATGGCC+AGG - chr5.3:3518356-3518375 MS.gene072743:CDS 45.0%
CATTATCTGGTCGGGTTTCA+AGG - chr5.3:3519510-3519529 MS.gene072743:CDS 45.0%
CTCAAAGGGCTCAATGCTAA+TGG + chr5.3:3520037-3520056 None:intergenic 45.0%
CTTCTGGTGTATGACCTTGA+AGG + chr5.3:3519893-3519912 None:intergenic 45.0%
GAAACCCGACCAGATAATGT+TGG + chr5.3:3519509-3519528 None:intergenic 45.0%
TAGTGAGTCTGAGCCAACAA+AGG - chr5.3:3519155-3519174 MS.gene072743:CDS 45.0%
TCTCATGCTTCAAATGGCCA+GGG - chr5.3:3518357-3518376 MS.gene072743:CDS 45.0%
TGAAACATGGCAGCATCTTC+TGG + chr5.3:3519909-3519928 None:intergenic 45.0%
TGAGAGAGAATTGTGGTCCA+AGG - chr5.3:3518299-3518318 MS.gene072743:CDS 45.0%
!! TGGAGGCCTTCTTTTAACTG+GGG + chr5.3:3519579-3519598 None:intergenic 45.0%
ACCCGACCAGATAATGTTGG+TGG + chr5.3:3519506-3519525 None:intergenic 50.0%
AGCATGAGATCAACCACCAC+AGG + chr5.3:3518346-3518365 None:intergenic 50.0%
CTCATGCTTCAAATGGCCAG+GGG - chr5.3:3518358-3518377 MS.gene072743:CDS 50.0%
GACCTTGAAGGACTCATTGG+CGG + chr5.3:3519881-3519900 None:intergenic 50.0%
GGCGAGCGATATGGAAACAA+AGG + chr5.3:3518784-3518803 None:intergenic 50.0%
GTCTGAGCCAACAAAGGATG+TGG - chr5.3:3519161-3519180 MS.gene072743:CDS 50.0%
GTGGAGATATTCAATGCCCC+TGG + chr5.3:3518377-3518396 None:intergenic 50.0%
CACCGCCAATGAGTCCTTCA+AGG - chr5.3:3519876-3519895 MS.gene072743:CDS 55.0%
GAGAAACCAGGACGCTGAGA+CGG + chr5.3:3518850-3518869 None:intergenic 55.0%
ACTGATCCGTCTCAGCGTCC+TGG - chr5.3:3518841-3518860 MS.gene072743:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 3518274 3520096 3518274 ID=MS.gene072743
chr5.3 mRNA 3518274 3520096 3518274 ID=MS.gene072743.t1;Parent=MS.gene072743
chr5.3 exon 3518995 3520096 3518995 ID=MS.gene072743.t1.exon1;Parent=MS.gene072743.t1
chr5.3 CDS 3518995 3520096 3518995 ID=cds.MS.gene072743.t1;Parent=MS.gene072743.t1
chr5.3 exon 3518274 3518899 3518274 ID=MS.gene072743.t1.exon2;Parent=MS.gene072743.t1
chr5.3 CDS 3518274 3518899 3518274 ID=cds.MS.gene072743.t1;Parent=MS.gene072743.t1
Gene Sequence

>MS.gene072743

ATGAACAAGGATTTGTGGACATACATGAGAGAGAATTGTGGTCCAAGGAGACAGATTTTGTTCTCTATACCTGTGGTGGTTGATCTCATGCTTCAAATGGCCAGGGGCATTGAATATCTCCACTCCAAAAACATTTACCATGGAAACCTTAATCCTTGTAATATCCTACTAAAAGCTAGAAACTCACAAGAAGGTTATTTTCAAGCAAAAGTTGTTGGATTTGGATTGTCATCTGTTGAAAATGGTGAAATTTATAATGCTTCTAGATCCTCACCAACACATAATCCAATTGGTGAAGAGATTAACCCTATAATTTGGTATGCACCTGAGGTTTTAACTGAGCTAGAACAAATGAAAAATGCTTCTACCTCTTGTAAGTACTCAGAGAAAGCCGATGCATATAGCTTTGGTATGATATGTTTTGAGTTGTTGACAGGGAAAATTCCTTTTGAGGATAATCATTTTCAAGGGATTAGAACCAACCAAAATATCAAAGCAGGAGAGAGGCCTTTGTTTCCATATCGCTCGCCAAAATATCTTGTGAGTTTGATCAAAAAATGTTGGCAAACTGATCCGTCTCAGCGTCCTGGTTTCTCATCTATTTGTAGGATTTTGCGCTACATCAAAAAGTTTCTTTCAATGAACACCGAGTATGTTTTGATCAATCCTGAACTAAATCAGCTTGAACTTCAGAACCCACCTGTAGATTGTTGTGACATTGAAGGAATTTTTCTCAAGAGTTTTCCTATGGAGAGGACATATAATATGTCTTGTGTTTCACAAATACCTTATGAAATGTTTGCTTATAAAGTTGTTGAAAAAGGGAAGATTAATAATCAGAATCTGAGTAATAATACTATTCCTACTATAGATAATAAGGGTAGTGAGTCTGAGCCAACAAAGGATGTGGCTACTGTTCGTAGCGAAGAAAGTGATGACCAAAGCACAGTTTGTGAAGATGTTGATGCATCCATAATTAAAGATCTGGTAACCTATCCAAAATCAATTTCTGATGACACACAGTCTATTTTTTATGATACCCCGTCAAAGAAGAATGTTACAATAAAGAAACCATCACAAATGAAGCCAAAGAAAGACCAAGGTCAGTTTAATTTTATTGTTATCTCAATTTTTCACCTGCTATATATATATATATATATATAATAAACTGATTATGTATTGAATTGATTGAAACAGGAACGCCTAAATTGCAAGCAACAAAATCATTACCACCAACATTATCTGGTCGGGTTTCAAGGACAAATAAGGCGAACCAGTCATCATTGACATCAAGTTCTATAACCCCAGTTAAAAGAAGGCCTCCAAAAGTCTCAGAATCTGAGAGGAAATCCAATATTAACAAAGGGAATCAATCAGCATCAGTGTCAACTTCAAGCTCAGTTAGAAAAAAAGTATATGGAGGCAATGTCTCAGATTCTAAGCCTAGTTTAAATCTGAAAACAAGAGACCAATCACCATTTAACAGGTTAAAGACAAAAAAATCACAACTAACGATGAATTCTTCTTCGAGTCCAACAAGAATAAGACGTGTCAATAGTACTAACAACAGCATTGTGAGGATGCAAAAAGGGTTCATTTCAACACCGCCAATGAGTCCTTCAAGGTCATACACCAGAAGATGCTGCCATGTTTCAGATATGGATAGTTCTTTGAAGATACGAGGTAGATTGTCACCATTTCCATTGAGTCCTTTGAGTCCATATGTGAGCAATCAAAGGACTAGAAGAGGAAGCTTGTCACCATTAGCATTGAGCCCTTTGAGTCCTTATGTAAGAACAACATATGGTCATGTCTCAGATTAA
Protein sequence

>MS.gene072743.t1

MNKDLWTYMRENCGPRRQILFSIPVVVDLMLQMARGIEYLHSKNIYHGNLNPCNILLKARNSQEGYFQAKVVGFGLSSVENGEIYNASRSSPTHNPIGEEINPIIWYAPEVLTELEQMKNASTSCKYSEKADAYSFGMICFELLTGKIPFEDNHFQGIRTNQNIKAGERPLFPYRSPKYLVSLIKKCWQTDPSQRPGFSSICRILRYIKKFLSMNTEYVLINPELNQLELQNPPVDCCDIEGIFLKSFPMERTYNMSCVSQIPYEMFAYKVVEKGKINNQNLSNNTIPTIDNKGSESEPTKDVATVRSEESDDQSTVCEDVDASIIKDLVTYPKSISDDTQSIFYDTPSKKNVTIKKPSQMKPKKDQGTPKLQATKSLPPTLSGRVSRTNKANQSSLTSSSITPVKRRPPKVSESERKSNINKGNQSASVSTSSSVRKKVYGGNVSDSKPSLNLKTRDQSPFNRLKTKKSQLTMNSSSSPTRIRRVNSTNNSIVRMQKGFISTPPMSPSRSYTRRCCHVSDMDSSLKIRGRLSPFPLSPLSPYVSNQRTRRGSLSPLALSPLSPYVRTTYGHVSD