AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21647

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21647.t1 MTR_5g009600 93.617 893 20 1 1 856 1 893 0.0 1721
MS.gene21647.t1 MTR_8g027990 47.099 741 320 15 1 730 1 680 0.0 640
MS.gene21647.t1 MTR_4g082325 29.773 309 168 15 247 539 142 417 1.19e-24 108
MS.gene21647.t1 MTR_4g132860 31.474 251 130 9 280 525 124 337 1.33e-24 107
MS.gene21647.t1 MTR_4g105770 29.845 258 142 10 282 536 104 325 1.26e-23 103
MS.gene21647.t1 MTR_1g026160 26.587 252 146 8 282 531 188 402 1.97e-23 104
MS.gene21647.t1 MTR_4g116520 30.556 288 150 11 264 535 1110 1363 2.58e-23 107
MS.gene21647.t1 MTR_3g078000 30.233 258 141 9 282 536 103 324 2.65e-23 102
MS.gene21647.t1 MTR_5g023150 26.104 249 146 8 282 528 240 452 4.53e-23 103
MS.gene21647.t1 MTR_5g023150 26.104 249 146 8 282 528 337 549 5.97e-23 104
MS.gene21647.t1 MTR_4g088745 27.273 253 145 10 282 531 92 308 4.29e-22 99.4
MS.gene21647.t1 MTR_4g088755 27.273 253 145 10 282 531 87 303 4.85e-22 99.0
MS.gene21647.t1 MTR_4g088750 26.616 263 155 9 282 541 248 475 8.25e-22 100
MS.gene21647.t1 MTR_2g461180 29.373 303 166 12 264 549 1145 1416 8.27e-22 102
MS.gene21647.t1 MTR_1g113960 26.908 249 144 8 282 528 343 555 1.04e-21 100
MS.gene21647.t1 MTR_1g113960 26.908 249 144 8 282 528 343 555 1.04e-21 100
MS.gene21647.t1 MTR_5g010730 28.247 308 163 17 246 534 421 689 1.26e-21 100
MS.gene21647.t1 MTR_5g010730 28.013 307 165 16 246 534 476 744 1.96e-21 100
MS.gene21647.t1 MTR_2g013900 25.954 262 158 9 282 541 209 436 9.85e-21 96.7
MS.gene21647.t1 MTR_6g033130 31.618 272 152 8 270 536 1001 1243 1.30e-20 98.6
MS.gene21647.t1 MTR_6g007603 28.137 263 143 10 279 526 118 349 1.35e-20 95.1
MS.gene21647.t1 MTR_7g072710 29.134 254 143 9 278 523 45 269 1.43e-20 94.4
MS.gene21647.t1 MTR_4g106980 26.996 263 146 8 279 526 135 366 1.90e-20 95.1
MS.gene21647.t1 MTR_7g072710 29.134 254 143 9 278 523 82 306 2.14e-20 94.4
MS.gene21647.t1 MTR_3g078110 25.779 353 192 13 212 526 17 337 2.45e-20 94.4
MS.gene21647.t1 MTR_1g099110 24.315 292 167 10 245 519 256 510 4.73e-20 95.1
MS.gene21647.t1 MTR_8g442400 26.687 326 185 11 222 526 1360 1652 5.81e-20 96.7
MS.gene21647.t1 MTR_4g128650 29.153 295 163 10 247 524 810 1075 6.06e-20 96.3
MS.gene21647.t1 MTR_1g099110 24.315 292 167 10 245 519 273 527 7.85e-20 94.7
MS.gene21647.t1 MTR_3g114900 27.092 251 145 9 282 530 312 526 9.29e-20 94.4
MS.gene21647.t1 MTR_4g122160 28.772 285 152 13 246 516 458 705 7.12e-18 89.0
MS.gene21647.t1 MTR_2g034020 27.857 280 145 14 265 528 485 723 1.42e-16 84.7
MS.gene21647.t1 MTR_3g034100 25.101 247 146 9 280 523 126 336 1.49e-16 82.8
MS.gene21647.t1 MTR_3g076970 25.413 303 169 11 242 523 20 286 1.59e-16 82.4
MS.gene21647.t1 MTR_1g090020 28.629 248 139 7 289 530 955 1170 2.10e-16 84.7
MS.gene21647.t1 MTR_8g100085 25.101 247 146 9 280 523 177 387 2.46e-16 82.8
MS.gene21647.t1 MTR_1g050520 28.696 230 115 9 326 526 106 315 7.99e-16 82.4
MS.gene21647.t1 MTR_5g019700 27.016 248 152 9 279 526 86 304 9.28e-16 80.9
MS.gene21647.t1 MTR_5g091880 28.253 269 155 11 280 541 205 442 9.87e-16 81.3
MS.gene21647.t1 MTR_5g006560 23.954 263 158 7 279 526 129 364 5.21e-15 78.6
MS.gene21647.t1 MTR_1g081790 24.252 301 173 13 246 530 687 948 8.21e-15 79.3
MS.gene21647.t1 MTR_5g006560 27.717 184 106 5 343 526 242 398 1.20e-14 77.4
MS.gene21647.t1 MTR_5g066490 25.581 301 165 15 247 528 645 905 1.50e-14 78.6
MS.gene21647.t1 MTR_1g026160 25.789 190 105 6 282 470 188 342 2.45e-14 75.9
MS.gene21647.t1 MTR_1g026160 25.789 190 105 6 282 470 188 342 2.46e-14 76.3
MS.gene21647.t1 MTR_1g026160 25.789 190 105 6 282 470 186 340 2.75e-14 75.9
MS.gene21647.t1 MTR_1g026160 25.789 190 105 6 282 470 186 340 2.92e-14 75.9
MS.gene21647.t1 MTR_1g026160 25.789 190 105 6 282 470 333 487 3.54e-14 76.6
MS.gene21647.t1 MTR_1g026160 25.789 190 105 6 282 470 333 487 3.97e-14 76.3
MS.gene21647.t1 MTR_1g061190 24.497 298 172 12 247 528 554 814 1.27e-13 75.5
MS.gene21647.t1 MTR_3g465590 26.250 240 138 8 289 523 375 580 1.28e-13 75.1
MS.gene21647.t1 MTR_5g083480 29.694 229 114 9 282 509 536 718 2.39e-13 74.7
MS.gene21647.t1 MTR_4g005730 23.288 292 182 10 243 521 19 281 3.28e-13 73.9
MS.gene21647.t1 MTR_4g005730 23.288 292 182 10 243 521 19 281 3.39e-13 73.9
MS.gene21647.t1 MTR_5g083480 29.694 229 114 9 282 509 539 721 3.46e-13 73.9
MS.gene21647.t1 MTR_4g005730 23.288 292 182 10 243 521 19 281 3.65e-13 73.9
MS.gene21647.t1 MTR_8g088590 27.165 254 126 10 282 526 236 439 1.37e-12 71.2
MS.gene21647.t1 MTR_3g073250 26.778 239 137 8 277 513 309 511 1.55e-12 71.6
MS.gene21647.t1 MTR_1g050690 28.000 275 149 13 265 526 436 674 1.88e-12 71.6
MS.gene21647.t1 MTR_8g058250 26.733 303 147 16 255 523 674 935 7.38e-12 69.7
MS.gene21647.t1 MTR_4g106980 26.761 213 111 7 279 476 135 317 7.45e-12 68.2
MS.gene21647.t1 MTR_6g006770 23.675 283 178 9 243 514 11 266 8.93e-12 69.3
MS.gene21647.t1 MTR_4g094415 27.350 234 130 7 283 514 211 406 9.04e-12 68.9
MS.gene21647.t1 MTR_8g058250 26.733 303 147 16 255 523 578 839 9.06e-12 69.7
MS.gene21647.t1 MTR_1g026160 24.731 186 104 6 282 466 186 336 9.16e-12 67.8
MS.gene21647.t1 MTR_4g094958 24.702 336 170 14 239 523 458 761 1.15e-11 68.9
MS.gene21647.t1 MTR_2g010470 26.190 252 131 11 241 469 676 895 1.69e-11 68.6
MS.gene21647.t1 MTR_1g110280 24.752 303 166 13 255 527 368 638 2.34e-11 67.8
MS.gene21647.t1 MTR_1g110270 25.869 259 158 9 277 524 354 589 5.75e-11 66.6
MS.gene21647.t1 MTR_4g080030 26.316 285 160 13 257 531 559 803 6.47e-11 66.6
MS.gene21647.t1 MTR_5g055470 25.769 260 144 11 277 521 377 602 6.48e-11 66.6
MS.gene21647.t1 MTR_8g093730 26.293 232 133 8 285 514 349 544 7.00e-11 66.6
MS.gene21647.t1 MTR_7g117520 26.820 261 133 12 286 523 463 688 8.39e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21647.t1 AT1G64300 53.590 752 290 14 1 730 1 715 0.0 784
MS.gene21647.t1 AT1G64300 53.590 752 290 14 1 730 1 715 0.0 784
MS.gene21647.t1 AT5G41730 53.039 724 290 10 1 721 1 677 0.0 764
MS.gene21647.t1 AT5G41730 53.039 724 290 10 1 721 1 677 0.0 764
MS.gene21647.t1 AT1G01450 53.590 390 151 7 211 594 2 367 1.05e-131 402
MS.gene21647.t1 AT1G14000 31.392 309 165 17 247 540 161 437 1.72e-24 107
MS.gene21647.t1 AT1G62400 30.924 249 129 9 280 523 130 340 5.03e-24 105
MS.gene21647.t1 AT3G46930 25.959 339 198 10 224 545 179 481 1.01e-23 106
MS.gene21647.t1 AT3G46930 25.959 339 198 10 224 545 144 446 1.43e-23 105
MS.gene21647.t1 AT3G46920 28.378 370 191 16 192 530 750 1076 1.31e-22 104
MS.gene21647.t1 AT3G46920 28.378 370 191 16 192 530 816 1142 1.41e-22 104
MS.gene21647.t1 AT5G58950 27.451 255 140 11 276 523 247 463 1.61e-21 99.8
MS.gene21647.t1 AT2G35050 28.618 304 166 14 242 526 968 1239 4.97e-21 99.8
MS.gene21647.t1 AT2G35050 28.618 304 166 14 242 526 968 1239 4.97e-21 99.8
MS.gene21647.t1 AT5G01850 27.642 246 145 7 282 523 63 279 8.43e-21 95.1
MS.gene21647.t1 AT5G57610 27.723 303 173 10 247 532 780 1053 2.89e-20 97.4
MS.gene21647.t1 AT4G38470 25.794 252 149 8 282 531 332 547 2.95e-20 95.9
MS.gene21647.t1 AT5G01850 29.907 214 120 6 314 523 116 303 1.12e-19 92.0
MS.gene21647.t1 AT4G35780 24.590 244 146 8 282 523 334 541 1.15e-19 94.4
MS.gene21647.t1 AT4G38470 25.506 247 146 8 282 526 332 542 1.54e-19 94.0
MS.gene21647.t1 AT3G01490 28.244 262 144 11 279 526 154 385 1.75e-19 92.4
MS.gene21647.t1 AT5G50000 26.616 263 147 8 279 526 128 359 1.91e-19 92.0
MS.gene21647.t1 AT1G04700 30.380 237 137 6 289 524 821 1030 2.28e-19 94.4
MS.gene21647.t1 AT1G04700 30.380 237 137 6 289 524 821 1030 2.28e-19 94.4
MS.gene21647.t1 AT2G17700 26.000 250 145 10 282 528 328 540 3.49e-19 92.8
MS.gene21647.t1 AT3G24715 28.873 284 172 9 243 522 842 1099 3.51e-19 94.0
MS.gene21647.t1 AT3G24715 28.873 284 172 9 243 522 842 1099 3.51e-19 94.0
MS.gene21647.t1 AT3G24715 28.873 284 172 9 243 522 842 1099 3.51e-19 94.0
MS.gene21647.t1 AT1G16270 27.143 280 167 8 258 533 890 1136 4.37e-19 93.6
MS.gene21647.t1 AT1G16270 27.143 280 167 8 258 533 890 1136 4.37e-19 93.6
MS.gene21647.t1 AT1G16270 27.143 280 167 8 258 533 890 1136 4.37e-19 93.6
MS.gene21647.t1 AT1G79570 26.403 303 175 10 242 526 958 1230 4.40e-19 93.6
MS.gene21647.t1 AT1G79570 26.403 303 175 10 242 526 958 1230 4.40e-19 93.6
MS.gene21647.t1 AT4G31170 27.092 251 136 9 280 523 175 385 5.25e-19 90.9
MS.gene21647.t1 AT4G31170 27.092 251 136 9 280 523 175 385 5.25e-19 90.9
MS.gene21647.t1 AT4G31170 27.092 251 136 9 280 523 175 385 5.25e-19 90.9
MS.gene21647.t1 AT4G31170 27.092 251 136 9 280 523 175 385 5.25e-19 90.9
MS.gene21647.t1 AT4G31170 27.092 251 136 9 280 523 175 385 5.25e-19 90.9
MS.gene21647.t1 AT4G31170 27.092 251 136 9 280 523 175 385 5.25e-19 90.9
MS.gene21647.t1 AT4G31170 27.092 251 136 9 280 523 175 385 5.25e-19 90.9
MS.gene21647.t1 AT3G50730 24.710 259 166 8 277 534 77 307 1.75e-18 89.0
MS.gene21647.t1 AT4G23050 27.815 302 158 14 246 528 465 725 2.98e-18 90.5
MS.gene21647.t1 AT3G06630 28.283 297 160 15 247 528 433 691 3.14e-18 90.1
MS.gene21647.t1 AT4G23050 27.815 302 158 14 246 528 466 726 3.30e-18 90.1
MS.gene21647.t1 AT5G66710 26.740 273 160 9 278 544 113 351 4.03e-18 88.2
MS.gene21647.t1 AT2G40860 30.964 197 98 6 340 514 24 204 9.43e-18 88.2
MS.gene21647.t1 AT5G49470 27.857 280 145 14 265 528 510 748 1.16e-17 88.6
MS.gene21647.t1 AT5G11850 25.503 298 171 12 246 529 607 867 1.38e-17 88.6
MS.gene21647.t1 AT2G40860 30.144 209 108 6 340 526 122 314 1.45e-17 87.8
MS.gene21647.t1 AT5G49470 27.857 280 145 14 265 528 484 722 1.46e-17 88.2
MS.gene21647.t1 AT2G40860 30.964 197 98 6 340 514 122 302 1.54e-17 87.8
MS.gene21647.t1 AT5G49470 27.857 280 145 14 265 528 383 621 1.64e-17 87.8
MS.gene21647.t1 AT3G27560 27.600 250 146 10 282 526 71 290 1.75e-17 85.5
MS.gene21647.t1 AT5G49470 27.857 280 145 14 265 528 223 461 1.81e-17 87.0
MS.gene21647.t1 AT2G40860 30.964 197 98 6 340 514 122 302 1.94e-17 87.4
MS.gene21647.t1 AT2G24360 26.050 238 143 7 286 523 180 384 2.22e-17 85.9
MS.gene21647.t1 AT5G40540 27.668 253 148 11 276 523 64 286 2.29e-17 85.1
MS.gene21647.t1 AT3G22750 24.528 265 151 8 279 526 120 352 3.21e-17 85.1
MS.gene21647.t1 AT3G57830 27.737 274 162 10 279 527 398 660 5.58e-17 86.3
MS.gene21647.t1 AT3G57830 27.737 274 162 10 279 527 398 660 5.58e-17 86.3
MS.gene21647.t1 AT4G18950 28.571 252 138 10 282 526 200 416 7.01e-17 84.7
MS.gene21647.t1 AT4G18950 28.571 252 138 10 282 526 200 416 7.05e-17 84.7
MS.gene21647.t1 AT3G63260 24.060 266 155 8 279 526 129 365 1.05e-16 83.6
MS.gene21647.t1 AT4G24480 26.073 303 166 13 247 528 668 933 1.56e-16 85.1
MS.gene21647.t1 AT2G42290 28.731 268 159 9 282 527 389 646 1.87e-16 84.3
MS.gene21647.t1 AT5G50180 27.236 246 145 9 282 523 65 280 2.13e-16 82.0
MS.gene21647.t1 AT1G67890 27.273 275 152 13 265 528 506 743 2.27e-16 84.3
MS.gene21647.t1 AT5G40540 29.439 214 120 8 314 523 3 189 5.07e-16 79.3
MS.gene21647.t1 AT4G24480 25.589 297 170 13 247 528 668 928 5.38e-16 83.2
MS.gene21647.t1 AT4G24480 25.589 297 170 13 247 528 668 928 5.50e-16 83.2
MS.gene21647.t1 AT5G40540 29.439 214 120 8 314 523 24 210 7.15e-16 79.3
MS.gene21647.t1 AT3G06620 27.798 277 149 13 265 528 513 751 8.16e-16 82.4
MS.gene21647.t1 AT3G50720 26.848 257 159 9 276 531 88 316 1.35e-15 80.1
MS.gene21647.t1 AT3G58760 29.000 300 156 18 247 526 165 427 6.71e-15 78.6
MS.gene21647.t1 AT3G58760 29.000 300 156 18 247 526 228 490 8.04e-15 79.0
MS.gene21647.t1 AT3G58760 29.000 300 156 18 247 526 228 490 8.04e-15 79.0
MS.gene21647.t1 AT3G06630 28.261 276 145 15 247 507 433 670 9.22e-15 79.0
MS.gene21647.t1 AT4G14780 26.316 190 109 5 339 526 178 338 1.09e-14 77.0
MS.gene21647.t1 AT2G31800 25.987 304 177 14 243 530 188 459 4.23e-14 76.3
MS.gene21647.t1 AT3G46930 24.715 263 149 8 224 473 179 405 4.40e-14 75.9
MS.gene21647.t1 AT3G46930 24.715 263 149 8 224 473 179 405 4.59e-14 75.9
MS.gene21647.t1 AT3G46930 24.715 263 149 8 224 473 144 370 6.07e-14 75.1
MS.gene21647.t1 AT3G46930 24.715 263 149 8 224 473 83 309 7.41e-14 74.3
MS.gene21647.t1 AT3G58640 28.205 273 146 13 257 519 565 797 9.68e-14 75.9
MS.gene21647.t1 AT3G58640 28.205 273 146 13 257 519 565 797 9.68e-14 75.9
MS.gene21647.t1 AT5G49470 30.729 192 96 9 343 528 649 809 2.34e-13 74.7
MS.gene21647.t1 AT1G50240 24.211 285 161 12 243 513 1 244 2.87e-13 74.7
MS.gene21647.t1 AT1G50240 24.211 285 161 12 243 513 1 244 2.87e-13 74.7
MS.gene21647.t1 AT5G01890 23.743 358 209 13 191 527 646 960 4.11e-13 73.9
MS.gene21647.t1 AT5G66850 25.703 249 153 10 283 530 394 611 5.04e-13 73.6
MS.gene21647.t1 AT5G66850 25.703 249 153 10 283 530 394 611 5.12e-13 73.6
MS.gene21647.t1 AT3G06630 28.571 238 120 13 247 470 433 634 5.21e-13 73.2
MS.gene21647.t1 AT3G06630 28.571 238 120 13 247 470 433 634 5.21e-13 73.2
MS.gene21647.t1 AT3G06630 28.571 238 120 13 247 470 433 634 5.21e-13 73.2
MS.gene21647.t1 AT3G59830 27.586 290 162 13 247 519 193 451 5.36e-13 72.8
MS.gene21647.t1 AT3G06630 28.571 238 120 13 247 470 349 550 6.35e-13 72.8
MS.gene21647.t1 AT5G18700 25.847 236 142 10 285 520 46 248 9.28e-13 73.2
MS.gene21647.t1 AT1G67890 27.907 258 130 15 265 507 506 722 3.55e-12 70.9
MS.gene21647.t1 AT1G67890 27.907 258 130 15 265 507 506 722 3.55e-12 70.9
MS.gene21647.t1 AT1G67890 27.907 258 130 15 265 507 378 594 4.12e-12 70.5
MS.gene21647.t1 AT2G31010 25.685 292 159 13 257 538 531 774 5.24e-12 70.1
MS.gene21647.t1 AT2G31010 25.685 292 159 13 257 538 531 774 5.24e-12 70.1
MS.gene21647.t1 AT3G53930 27.586 261 134 13 247 491 19 240 6.64e-12 69.7
MS.gene21647.t1 AT3G53930 27.586 261 134 13 247 491 19 240 7.06e-12 69.7
MS.gene21647.t1 AT5G49470 28.182 220 104 12 265 469 510 690 1.12e-11 68.9
MS.gene21647.t1 AT5G49470 28.182 220 104 12 265 469 510 690 1.13e-11 68.9
MS.gene21647.t1 AT2G42640 28.044 271 141 13 265 524 547 774 1.18e-11 68.9
MS.gene21647.t1 AT2G42640 28.044 271 141 13 265 524 509 736 1.29e-11 68.9
MS.gene21647.t1 AT5G38210 24.739 287 162 10 280 544 391 645 1.63e-11 68.6
MS.gene21647.t1 AT5G38210 25.175 286 162 10 280 544 409 663 1.64e-11 68.6
MS.gene21647.t1 AT5G07140 26.014 296 177 13 242 528 321 583 2.26e-11 67.8
MS.gene21647.t1 AT3G01490 27.632 228 122 10 279 492 154 352 2.66e-11 66.6
MS.gene21647.t1 AT4G08480 26.172 256 147 9 261 514 529 744 4.45e-11 67.4
MS.gene21647.t1 AT1G69270 25.820 244 144 10 270 510 291 500 4.78e-11 66.6
MS.gene21647.t1 AT5G40540 28.061 196 114 8 276 469 64 234 4.90e-11 65.1
MS.gene21647.t1 AT3G02880 31.500 200 99 9 272 467 375 540 6.08e-11 66.6
MS.gene21647.t1 AT4G08500 26.336 262 153 9 255 514 353 576 6.53e-11 66.6
MS.gene21647.t1 AT5G38210 25.175 286 162 10 280 544 397 651 6.53e-11 66.6
MS.gene21647.t1 AT3G19700 28.497 193 105 8 282 469 730 894 7.43e-11 66.6

Find 153 sgRNAs with CRISPR-Local

Find 203 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCTCCTGCTTTGATATTT+TGG 0.080066 5.2:+3214281 None:intergenic
CTCTCAGATTCTGAGACTTT+TGG 0.146039 5.2:+3213416 None:intergenic
ATCAAACTCACAAGATATTT+TGG 0.164131 5.2:+3214235 None:intergenic
TTGATTCCCTTTGTTAATAT+TGG 0.196770 5.2:+3213388 None:intergenic
CAAGCAAAAGTTGTTGGATT+TGG 0.204645 5.2:-3214562 MS.gene21647:CDS
GAGATTTGGCAAACAATATT+TGG 0.205668 5.2:-3215235 MS.gene21647:CDS
GATCCTAACAAAACACTAAT+TGG 0.207436 5.2:+3215006 None:intergenic
AATTGCTTGCAAATTTCTTT+TGG 0.212048 5.2:+3215210 None:intergenic
TCTCCTCACTTGGTAATCTT+TGG 0.230174 5.2:+3214984 None:intergenic
GCTTATAAAGTTGTTGAAAA+AGG 0.240086 5.2:-3213962 MS.gene21647:CDS
GTGTCATCAGAAATTGATTT+TGG 0.241755 5.2:+3213767 None:intergenic
GTAGGATATTACAAGGATTA+AGG 0.243595 5.2:+3214617 None:intergenic
TATTCCTACTATAGATAATA+AGG 0.252638 5.2:-3213906 MS.gene21647:CDS
AAGAGATTAACCCTATAATT+TGG 0.257480 5.2:-3214468 MS.gene21647:CDS
GTCCTCTTAGAGAGCTTTCC+AGG 0.263161 5.2:-3215518 MS.gene21647:CDS
AACTGACCTTGGTCTTTCTT+TGG 0.265277 5.2:+3213677 None:intergenic
GGTTACCAGATCTTTAATTA+TGG 0.266121 5.2:+3213793 None:intergenic
CATAGATATTGCAATATGTT+TGG 0.282076 5.2:+3214835 None:intergenic
TCAGGTGCATACCAAATTAT+AGG 0.292831 5.2:+3214457 None:intergenic
CTGGTTTCTCATCTATTTGT+AGG 0.294229 5.2:-3214177 MS.gene21647:CDS
TTTGTCTTTAACCTGTTAAA+TGG 0.297516 5.2:+3213263 None:intergenic
CGGCACATTGGTGAGGTTCT+TGG 0.303933 5.2:-3215705 MS.gene21647:CDS
TATGGTCATGTCTCAGATTA+AGG 0.304997 5.2:-3212936 None:intergenic
TGGAAGTCTAAAAGCGCTTA+TGG 0.306735 5.2:-3215685 MS.gene21647:CDS
TGAAACATGGCAGCATCTTC+TGG 0.314429 5.2:+3213106 None:intergenic
AAAAGAAATTTGCAAGCAAT+TGG 0.319977 5.2:-3215208 MS.gene21647:CDS
GGATTGTCATCTGTTGAAAA+TGG 0.333696 5.2:-3214541 MS.gene21647:CDS
GTCCTTTGATTGCTCACATA+TGG 0.339488 5.2:+3213020 None:intergenic
AGCATTGTGAGGATGCAAAA+AGG 0.341134 5.2:-3213170 MS.gene21647:CDS
ATTATACATTAAGCATTGTT+TGG 0.350263 5.2:-3215478 MS.gene21647:CDS
TTGACACGTCTTATTCTTGT+TGG 0.355333 5.2:+3213206 None:intergenic
GAAATTGATGAATGGTAATT+CGG 0.363355 5.2:-3215070 MS.gene21647:CDS
TTTAAGGAAATTCAATGGTT+AGG 0.370820 5.2:-3214940 MS.gene21647:CDS
GAGACCAATCACCATTTAAC+AGG 0.371640 5.2:-3213274 MS.gene21647:CDS
ATTGTTTGGATTCAAAAGAT+TGG 0.378122 5.2:-3215464 MS.gene21647:CDS
GTGAGAAAGTGATAACAAAT+TGG 0.378799 5.2:+3214858 None:intergenic
TTCTTTGGCTTCATTTGTGA+TGG 0.379395 5.2:+3213692 None:intergenic
TTAACCTGTTAAATGGTGAT+TGG 0.382051 5.2:+3213270 None:intergenic
ATAGATATTGCAATATGTTT+GGG 0.387928 5.2:+3214836 None:intergenic
AGTTCAAGCTGATTTAGTTC+AGG 0.389760 5.2:+3214097 None:intergenic
TTGAAGACAGAACTACAAAA+TGG 0.399911 5.2:-3215554 MS.gene21647:CDS
ATTGTAACATTCTTCTTTGA+CGG 0.401100 5.2:+3213722 None:intergenic
TGAGGAGAAGATTGGGAAGG+GGG 0.403016 5.2:-3214969 MS.gene21647:CDS
GGGTTAATCTCTTCACCAAT+TGG 0.403263 5.2:+3214478 None:intergenic
CCATATGTTGTTCTTACATA+AGG 0.409101 5.2:+3212954 None:intergenic
TACCAAGTGAGGAGAAGATT+GGG 0.409714 5.2:-3214976 MS.gene21647:CDS
ACAGTAGCCACATCCTTTGT+TGG 0.413590 5.2:+3213869 None:intergenic
TTGGATTCAAAAGATTGGTC+AGG 0.420334 5.2:-3215459 MS.gene21647:CDS
ATGCTGCCATGTTTCAGATA+TGG 0.424537 5.2:-3213099 MS.gene21647:CDS
GAGAATGCTGGGGAGATGTC+AGG 0.427765 5.2:-3215345 MS.gene21647:CDS
TTATAAGCAAACATTTCATA+AGG 0.434328 5.2:+3213977 None:intergenic
CATCTTCACAAACTGTGCTT+TGG 0.436780 5.2:+3213825 None:intergenic
TGCATCCATAATTAAAGATC+TGG 0.439192 5.2:-3213798 MS.gene21647:CDS
TTGTAACATTCTTCTTTGAC+GGG 0.441892 5.2:+3213723 None:intergenic
CTCAAAGGGCTCAATGCTAA+TGG 0.444928 5.2:+3212978 None:intergenic
CTCAAAGGACTCAATGGAAA+TGG 0.446507 5.2:+3213044 None:intergenic
TTCCCAATCTTCTCCTCACT+TGG 0.447928 5.2:+3214974 None:intergenic
ATTCCTACTATAGATAATAA+GGG 0.450157 5.2:-3213905 MS.gene21647:CDS
TGAAAATGATTATCCTCAAA+AGG 0.452553 5.2:+3214319 None:intergenic
GCAAGCAATTGGAGAATGCT+TGG 0.459665 5.2:-3215197 MS.gene21647:CDS
GAGAACAAAATCTGTCTCCT+TGG 0.464084 5.2:+3214721 None:intergenic
GGAGATGTCAGGGTTGGATC+AGG 0.464317 5.2:-3215334 MS.gene21647:CDS
TTACCAAGTGAGGAGAAGAT+TGG 0.469923 5.2:-3214977 MS.gene21647:CDS
AGGAATTTAAGGAAATTCAA+TGG 0.476317 5.2:-3214945 MS.gene21647:CDS
CACCGCCAATGAGTCCTTCA+AGG 0.481111 5.2:-3213136 MS.gene21647:CDS
AAAGCCGATGCATATAGCTT+TGG 0.483467 5.2:-3214376 MS.gene21647:CDS
CAGGTGCATACCAAATTATA+GGG 0.486379 5.2:+3214458 None:intergenic
ACATTATATGTCCTCTCCAT+TGG 0.487393 5.2:+3214019 None:intergenic
TCACCAACACATAATCCAAT+TGG 0.489553 5.2:-3214493 MS.gene21647:CDS
TTCACCTTCTTTGAATACCC+TGG 0.491717 5.2:+3215500 None:intergenic
ATTCTTTGAAGAAGTGAGCT+TGG 0.493506 5.2:+3215122 None:intergenic
TACAAGGATTAAGGTTTCCA+TGG 0.498240 5.2:+3214626 None:intergenic
TATGGACTCAAAGGACTCAA+TGG 0.498751 5.2:+3213038 None:intergenic
CTACCCTTATTATCTATAGT+AGG 0.503856 5.2:+3213902 None:intergenic
ACCAACCAAAATATCAAAGC+AGG 0.507591 5.2:-3214286 MS.gene21647:CDS
GTTCTTACATAAGGACTCAA+AGG 0.508350 5.2:+3212963 None:intergenic
ATTGAAGCGATCGAGAATGC+TGG 0.508806 5.2:-3215357 MS.gene21647:CDS
GTGGAGATATTCAATGCCTC+TGG 0.511386 5.2:+3214663 None:intergenic
CTTTCCAGGGTATTCAAAGA+AGG 0.511780 5.2:-3215504 MS.gene21647:CDS
TCTAGCTCAGTTAAAACCTC+AGG 0.514317 5.2:+3214439 None:intergenic
TTGAAGCGATCGAGAATGCT+GGG 0.515187 5.2:-3215356 MS.gene21647:CDS
ACTGATCCGTCTCAGCGTCC+TGG 0.518950 5.2:-3214196 MS.gene21647:CDS
CTTCTGGTGTATGACCTTGA+AGG 0.519264 5.2:+3213122 None:intergenic
AGAATGCTTGGAGAGAAGAT+AGG 0.519476 5.2:-3215185 MS.gene21647:CDS
AGTTGATGAACAAGGATTTG+TGG 0.524686 5.2:-3214768 MS.gene21647:CDS
TGGATTCAAAAGATTGGTCA+GGG 0.524926 5.2:-3215458 MS.gene21647:CDS
TGCTGGGGAGATGTCAGGGT+TGG 0.526191 5.2:-3215340 MS.gene21647:CDS
GGAAATCCAATATTAACAAA+GGG 0.528711 5.2:-3213394 MS.gene21647:CDS
CCTTATGTAAGAACAACATA+TGG 0.529072 5.2:-3212954 MS.gene21647:CDS
CTATAACCCCAGTTAAAAGA+AGG 0.532741 5.2:-3213442 MS.gene21647:intron
AGGAAATCCAATATTAACAA+AGG 0.532942 5.2:-3213395 MS.gene21647:CDS
TATGACCTTGAAGGACTCAT+TGG 0.538589 5.2:+3213131 None:intergenic
CAAGAAAATATGATGTGGAA+TGG 0.539842 5.2:-3215281 MS.gene21647:CDS
ACACAATCGCAGATGAGATA+AGG 0.544064 5.2:-3215590 MS.gene21647:CDS
GTGCCGGAATGGAACAGCTC+CGG 0.546196 5.2:-3215725 None:intergenic
TTTGTTCTCTATACCTGTGG+TGG 0.547282 5.2:-3214707 MS.gene21647:CDS
GTAGATTGTTGTGACATTGA+AGG 0.548073 5.2:-3214061 MS.gene21647:CDS
ATGCTTGGAGAGAAGATAGG+TGG 0.548742 5.2:-3215182 MS.gene21647:CDS
TATAATTTGGTATGCACCTG+AGG 0.550991 5.2:-3214455 MS.gene21647:CDS
TTCTTACATAAGGACTCAAA+GGG 0.551714 5.2:+3212964 None:intergenic
GGTTGATCTCATGCTTCAAA+TGG 0.555778 5.2:-3214686 MS.gene21647:CDS
AACATATTGCAATATCTATG+TGG 0.560041 5.2:-3214832 MS.gene21647:CDS
ACCCTGGAAAGCTCTCTAAG+AGG 0.560256 5.2:+3215516 None:intergenic
ATGGAACAGCTCCGGCACAT+TGG 0.561500 5.2:-3215717 MS.gene21647:CDS
AGCAATCAAAGGACTAGAAG+AGG 0.562315 5.2:-3213011 MS.gene21647:CDS
GGCGAGCGATATGGAAACAA+AGG 0.564210 5.2:+3214256 None:intergenic
CAAGAACCTCACCAATGTGC+CGG 0.564531 5.2:+3215706 None:intergenic
TCAATGTCACAACAATCTAC+AGG 0.568956 5.2:+3214064 None:intergenic
GACCTTGAAGGACTCATTGG+CGG 0.570717 5.2:+3213134 None:intergenic
TTGTTGAAGAAATTGATGAA+TGG 0.570718 5.2:-3215078 MS.gene21647:CDS
TCACCAATTGGATTATGTGT+TGG 0.571772 5.2:+3214490 None:intergenic
GAGAAACCAGGACGCTGAGA+CGG 0.572704 5.2:+3214190 None:intergenic
GTCTGAGCCAACAAAGGATG+TGG 0.574953 5.2:-3213876 MS.gene21647:CDS
TTGCTCACATATGGACTCAA+AGG 0.582014 5.2:+3213029 None:intergenic
AGTGAGGAGAAGATTGGGAA+GGG 0.587219 5.2:-3214971 MS.gene21647:CDS
TCGATCGCTTCAATAACCGC+AGG 0.587690 5.2:+3215366 None:intergenic
GTGAGGAGAAGATTGGGAAG+GGG 0.591731 5.2:-3214970 MS.gene21647:CDS
GAGGCTCATAGAAGCACTCA+AGG 0.595114 5.2:-3215160 MS.gene21647:CDS
GTCCATATGTGAGCAATCAA+AGG 0.596259 5.2:-3213022 MS.gene21647:CDS
CATACCAAAGCTATATGCAT+CGG 0.599646 5.2:+3214372 None:intergenic
AAAGCTAGAAACTCACAAGA+AGG 0.600879 5.2:-3214592 MS.gene21647:CDS
AGAATGCTGGGGAGATGTCA+GGG 0.601811 5.2:-3215344 MS.gene21647:CDS
TGAGAGAGAATTGTGGTCCA+AGG 0.604253 5.2:-3214738 MS.gene21647:CDS
TCCTCTTAGAGAGCTTTCCA+GGG 0.606681 5.2:-3215517 MS.gene21647:CDS
TTTCTCTGAGTACTTACAAG+AGG 0.613541 5.2:+3214396 None:intergenic
AGCATGAGATCAACCACCAC+AGG 0.618436 5.2:+3214694 None:intergenic
CTTGGAGAGAAGATAGGTGG+AGG 0.618932 5.2:-3215179 MS.gene21647:CDS
AAAATATCAAAGCAGGAGAG+AGG 0.620693 5.2:-3214279 MS.gene21647:CDS
CTACAAATAGATGAGAAACC+AGG 0.623694 5.2:+3214178 None:intergenic
AACTATCCATATCTGAAACA+TGG 0.625924 5.2:+3213093 None:intergenic
ACAGTAATGATAAGGTATTG+TGG 0.632731 5.2:-3215029 MS.gene21647:CDS
ATGTCACAACAATCTACAGG+TGG 0.639302 5.2:+3214067 None:intergenic
ATGCTAGAGTGAATATGTCG+TGG 0.640538 5.2:+3215616 None:intergenic
AGGATTGATCAAAACATACT+CGG 0.640765 5.2:+3214117 None:intergenic
CAAAAGTCTCAGAATCTGAG+AGG 0.650694 5.2:-3213415 MS.gene21647:CDS
TCTCTAAGAGGACATTCAAG+AGG 0.651164 5.2:+3215528 None:intergenic
ACAGCTCCGGCACATTGGTG+AGG 0.651948 5.2:-3215712 MS.gene21647:CDS
GCTTGCAAGAAAATATGATG+TGG 0.653459 5.2:-3215286 MS.gene21647:CDS
TAGTGAGTCTGAGCCAACAA+AGG 0.653550 5.2:-3213882 MS.gene21647:CDS
CGATCGCTTCAATAACCGCA+GGG 0.654532 5.2:+3215367 None:intergenic
AAGTGAGGAGAAGATTGGGA+AGG 0.655591 5.2:-3214972 MS.gene21647:CDS
AATTGGATTATGTGTTGGTG+AGG 0.655784 5.2:+3214495 None:intergenic
TGTGACGGAGTTGATGAACA+AGG 0.656381 5.2:-3214776 MS.gene21647:CDS
CTCATGCTTCAAATGGCCAG+AGG 0.666006 5.2:-3214679 MS.gene21647:CDS
GATCCAAAGATTACCAAGTG+AGG 0.673638 5.2:-3214987 MS.gene21647:CDS
ATGAAGCCAAAGAAAGACCA+AGG 0.681190 5.2:-3213683 MS.gene21647:intron
TGTAACATTCTTCTTTGACG+GGG 0.687811 5.2:+3213724 None:intergenic
GTACTAACAACAGCATTGTG+AGG 0.690491 5.2:-3213181 MS.gene21647:CDS
GATAGTTCTTTGAAGATACG+AGG 0.692687 5.2:-3213077 MS.gene21647:CDS
TGTCACAACAATCTACAGGT+GGG 0.697110 5.2:+3214068 None:intergenic
CTTGCTTTGCTATTTCCCTG+CGG 0.700742 5.2:-3215382 MS.gene21647:CDS
TGAAGCGATCGAGAATGCTG+GGG 0.704654 5.2:-3215355 MS.gene21647:CDS
ACATACATGAGAGAGAATTG+TGG 0.732943 5.2:-3214745 MS.gene21647:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATATATATATATAGC+AGG + chr5.2:3215030-3215049 None:intergenic 10.0%
!! ATAGATATTGCAATATGTTT+GGG + chr5.2:3213822-3213841 None:intergenic 20.0%
!! ATTCCTACTATAGATAATAA+GGG - chr5.2:3214750-3214769 MS.gene21647:CDS 20.0%
!! CTTATAAAGTTGTTGAAAAA+GGG - chr5.2:3214694-3214713 MS.gene21647:CDS 20.0%
!! TAATAAAATTAAACTGACCT+TGG + chr5.2:3214992-3215011 None:intergenic 20.0%
!! TATTCCTACTATAGATAATA+AGG - chr5.2:3214749-3214768 MS.gene21647:CDS 20.0%
!! TTATAAGCAAACATTTCATA+AGG + chr5.2:3214681-3214700 None:intergenic 20.0%
!!! ATTATACATTAAGCATTGTT+TGG - chr5.2:3213177-3213196 MS.gene21647:CDS 20.0%
!!! TCAGTTAGAAAAAAAGTATA+TGG - chr5.2:3215314-3215333 MS.gene21647:CDS 20.0%
! AAAAGAAATTTGCAAGCAAT+TGG - chr5.2:3213447-3213466 MS.gene21647:intron 25.0%
! AACATATTGCAATATCTATG+TGG - chr5.2:3213823-3213842 MS.gene21647:CDS 25.0%
! AAGAGATTAACCCTATAATT+TGG - chr5.2:3214187-3214206 MS.gene21647:CDS 25.0%
! AGGAAATCCAATATTAACAA+AGG - chr5.2:3215260-3215279 MS.gene21647:CDS 25.0%
! AGGAATTTAAGGAAATTCAA+TGG - chr5.2:3213710-3213729 MS.gene21647:CDS 25.0%
! ATTGTAACATTCTTCTTTGA+CGG + chr5.2:3214936-3214955 None:intergenic 25.0%
! ATTGTTTGGATTCAAAAGAT+TGG - chr5.2:3213191-3213210 MS.gene21647:CDS 25.0%
! CATAGATATTGCAATATGTT+TGG + chr5.2:3213823-3213842 None:intergenic 25.0%
! GAAATTGATGAATGGTAATT+CGG - chr5.2:3213585-3213604 MS.gene21647:intron 25.0%
! GCTTATAAAGTTGTTGAAAA+AGG - chr5.2:3214693-3214712 MS.gene21647:CDS 25.0%
! GGAAATCCAATATTAACAAA+GGG - chr5.2:3215261-3215280 MS.gene21647:CDS 25.0%
! TCACAAAAACAGTAATGATA+AGG - chr5.2:3213618-3213637 MS.gene21647:intron 25.0%
! TGAAAATGATTATCCTCAAA+AGG + chr5.2:3214339-3214358 None:intergenic 25.0%
! TGAGTTTGATCAAAAAATGT+TGG - chr5.2:3214433-3214452 MS.gene21647:CDS 25.0%
! TGTATTGAATTGATTGAAAC+AGG - chr5.2:3215092-3215111 MS.gene21647:CDS 25.0%
! TTGATTCCCTTTGTTAATAT+TGG + chr5.2:3215270-3215289 None:intergenic 25.0%
! TTGTTGAAGAAATTGATGAA+TGG - chr5.2:3213577-3213596 MS.gene21647:intron 25.0%
! TTTAAGGAAATTCAATGGTT+AGG - chr5.2:3213715-3213734 MS.gene21647:CDS 25.0%
! TTTGTCTTTAACCTGTTAAA+TGG + chr5.2:3215395-3215414 None:intergenic 25.0%
!! AATTGCTTGCAAATTTCTTT+TGG + chr5.2:3213448-3213467 None:intergenic 25.0%
!! ATCAAACTCACAAGATATTT+TGG + chr5.2:3214423-3214442 None:intergenic 25.0%
!! GTTAGAAAAAAAGTATATGG+AGG - chr5.2:3215317-3215336 MS.gene21647:CDS 25.0%
!! TTGAGGATAATCATTTTCAA+GGG - chr5.2:3214340-3214359 MS.gene21647:CDS 25.0%
!! TTTGAGGATAATCATTTTCA+AGG - chr5.2:3214339-3214358 MS.gene21647:CDS 25.0%
!!! ATCAGAAATTGATTTTGGAT+AGG + chr5.2:3214886-3214905 None:intergenic 25.0%
!!! CTTGTTTTCAGATTTAAACT+AGG + chr5.2:3215362-3215381 None:intergenic 25.0%
!!! TATTTTCAAGCAAAAGTTGT+TGG - chr5.2:3214087-3214106 MS.gene21647:CDS 25.0%
AACTTGATGTCAATGATTAC+TGG + chr5.2:3215193-3215212 None:intergenic 30.0%
AGGATTGATCAAAACATACT+CGG + chr5.2:3214541-3214560 None:intergenic 30.0%
CAAGAAAATATGATGTGGAA+TGG - chr5.2:3213374-3213393 MS.gene21647:CDS 30.0%
CCATATGTTGTTCTTACATA+AGG + chr5.2:3215704-3215723 None:intergenic 30.0%
CCTTATGTAAGAACAACATA+TGG - chr5.2:3215701-3215720 MS.gene21647:CDS 30.0%
CTACCCTTATTATCTATAGT+AGG + chr5.2:3214756-3214775 None:intergenic 30.0%
GAGATTTGGCAAACAATATT+TGG - chr5.2:3213420-3213439 MS.gene21647:CDS 30.0%
GATCCTAACAAAACACTAAT+TGG + chr5.2:3213652-3213671 None:intergenic 30.0%
GGTTACCAGATCTTTAATTA+TGG + chr5.2:3214865-3214884 None:intergenic 30.0%
GTAGGATATTACAAGGATTA+AGG + chr5.2:3214041-3214060 None:intergenic 30.0%
GTGAGAAAGTGATAACAAAT+TGG + chr5.2:3213800-3213819 None:intergenic 30.0%
TGCATCCATAATTAAAGATC+TGG - chr5.2:3214857-3214876 MS.gene21647:CDS 30.0%
TTAACCTGTTAAATGGTGAT+TGG + chr5.2:3215388-3215407 None:intergenic 30.0%
TTCTTACATAAGGACTCAAA+GGG + chr5.2:3215694-3215713 None:intergenic 30.0%
TTGAAGACAGAACTACAAAA+TGG - chr5.2:3213101-3213120 MS.gene21647:CDS 30.0%
TTGTAACATTCTTCTTTGAC+GGG + chr5.2:3214935-3214954 None:intergenic 30.0%
! AACTATCCATATCTGAAACA+TGG + chr5.2:3215565-3215584 None:intergenic 30.0%
! AATCTCCACTGAAAAAGTTT+TGG + chr5.2:3213406-3213425 None:intergenic 30.0%
! ATTTCTCAAGAGTTTTCCAA+TGG - chr5.2:3214620-3214639 MS.gene21647:CDS 30.0%
! GCTTTTAGTAGGATATTACA+AGG + chr5.2:3214048-3214067 None:intergenic 30.0%
! GTTTCCATGGTAAATGTTTT+TGG + chr5.2:3214019-3214038 None:intergenic 30.0%
!! ACAGTAATGATAAGGTATTG+TGG - chr5.2:3213626-3213645 MS.gene21647:intron 30.0%
!! GATTTTGTTGCTTGCAATTT+AGG + chr5.2:3215122-3215141 None:intergenic 30.0%
!! GTGTCATCAGAAATTGATTT+TGG + chr5.2:3214891-3214910 None:intergenic 30.0%
!!! AAACTTTTTCAGTGGAGATT+TGG - chr5.2:3213406-3213425 MS.gene21647:CDS 30.0%
!!! ATATGTTTTGAGTTGTTGAC+AGG - chr5.2:3214306-3214325 MS.gene21647:CDS 30.0%
!!! ATGATCCAAAACTTTTTCAG+TGG - chr5.2:3213398-3213417 MS.gene21647:CDS 30.0%
!!! TATGTTTTGAGTTGTTGACA+GGG - chr5.2:3214307-3214326 MS.gene21647:CDS 30.0%
AAAATATCAAAGCAGGAGAG+AGG - chr5.2:3214376-3214395 MS.gene21647:CDS 35.0%
AAAGCTAGAAACTCACAAGA+AGG - chr5.2:3214063-3214082 MS.gene21647:CDS 35.0%
ACATACATGAGAGAGAATTG+TGG - chr5.2:3213910-3213929 MS.gene21647:CDS 35.0%
ACATTATATGTCCTCTCCAT+TGG + chr5.2:3214639-3214658 None:intergenic 35.0%
ACCAACCAAAATATCAAAGC+AGG - chr5.2:3214369-3214388 MS.gene21647:CDS 35.0%
CAAGCAAAAGTTGTTGGATT+TGG - chr5.2:3214093-3214112 MS.gene21647:CDS 35.0%
CACTCCAAAAACATTTACCA+TGG - chr5.2:3214012-3214031 MS.gene21647:CDS 35.0%
CAGGTGCATACCAAATTATA+GGG + chr5.2:3214200-3214219 None:intergenic 35.0%
CATACCAAAGCTATATGCAT+CGG + chr5.2:3214286-3214305 None:intergenic 35.0%
CTACAAATAGATGAGAAACC+AGG + chr5.2:3214480-3214499 None:intergenic 35.0%
CTATAACCCCAGTTAAAAGA+AGG - chr5.2:3215213-3215232 MS.gene21647:CDS 35.0%
CTGGTTTCTCATCTATTTGT+AGG - chr5.2:3214478-3214497 MS.gene21647:CDS 35.0%
GCTTGCAAGAAAATATGATG+TGG - chr5.2:3213369-3213388 MS.gene21647:CDS 35.0%
GGATTGTCATCTGTTGAAAA+TGG - chr5.2:3214114-3214133 MS.gene21647:CDS 35.0%
GTAGATTGTTGTGACATTGA+AGG - chr5.2:3214594-3214613 MS.gene21647:CDS 35.0%
GTTCTTACATAAGGACTCAA+AGG + chr5.2:3215695-3215714 None:intergenic 35.0%
TACAAGGATTAAGGTTTCCA+TGG + chr5.2:3214032-3214051 None:intergenic 35.0%
TATAATTTGGTATGCACCTG+AGG - chr5.2:3214200-3214219 MS.gene21647:CDS 35.0%
TCAATGTCACAACAATCTAC+AGG + chr5.2:3214594-3214613 None:intergenic 35.0%
TCACCAACACATAATCCAAT+TGG - chr5.2:3214162-3214181 MS.gene21647:CDS 35.0%
TCACCAATTGGATTATGTGT+TGG + chr5.2:3214168-3214187 None:intergenic 35.0%
TCAGGTGCATACCAAATTAT+AGG + chr5.2:3214201-3214220 None:intergenic 35.0%
TCATTACCACCAACATTATC+TGG - chr5.2:3215140-3215159 MS.gene21647:CDS 35.0%
TGTAACATTCTTCTTTGACG+GGG + chr5.2:3214934-3214953 None:intergenic 35.0%
TTTCTCTGAGTACTTACAAG+AGG + chr5.2:3214262-3214281 None:intergenic 35.0%
! AATTGGATTATGTGTTGGTG+AGG + chr5.2:3214163-3214182 None:intergenic 35.0%
! AGTTCAAGCTGATTTAGTTC+AGG + chr5.2:3214561-3214580 None:intergenic 35.0%
! ATTCTTTGAAGAAGTGAGCT+TGG + chr5.2:3213536-3213555 None:intergenic 35.0%
! GAAAGAGTTTTCTCTTGTGA+CGG - chr5.2:3213864-3213883 MS.gene21647:CDS 35.0%
! GATAGTTCTTTGAAGATACG+AGG - chr5.2:3215578-3215597 MS.gene21647:CDS 35.0%
! GATTTTGTTCTCTATACCTG+TGG - chr5.2:3213945-3213964 MS.gene21647:CDS 35.0%
! TCTCTCCTGCTTTGATATTT+TGG + chr5.2:3214377-3214396 None:intergenic 35.0%
! TGGATTCAAAAGATTGGTCA+GGG - chr5.2:3213197-3213216 MS.gene21647:CDS 35.0%
! TTCTTTGGCTTCATTTGTGA+TGG + chr5.2:3214966-3214985 None:intergenic 35.0%
! TTGACACGTCTTATTCTTGT+TGG + chr5.2:3215452-3215471 None:intergenic 35.0%
! TTGGATTCAAAAGATTGGTC+AGG - chr5.2:3213196-3213215 MS.gene21647:CDS 35.0%
!! AGTTGATGAACAAGGATTTG+TGG - chr5.2:3213887-3213906 MS.gene21647:CDS 35.0%
!! TGGCCAATTAGTGTTTTGTT+AGG - chr5.2:3213646-3213665 MS.gene21647:intron 35.0%
!!! CATGGTAAATGTTTTTGGAG+TGG + chr5.2:3214014-3214033 None:intergenic 35.0%
!!! GTGAGTTTCTAGCTTTTAGT+AGG + chr5.2:3214059-3214078 None:intergenic 35.0%
!!! TCCTGCTTTGATATTTTGGT+TGG + chr5.2:3214373-3214392 None:intergenic 35.0%
AAAGCCGATGCATATAGCTT+TGG - chr5.2:3214279-3214298 MS.gene21647:CDS 40.0%
ACACAATCGCAGATGAGATA+AGG - chr5.2:3213065-3213084 MS.gene21647:CDS 40.0%
AGAATGCTTGGAGAGAAGAT+AGG - chr5.2:3213470-3213489 MS.gene21647:intron 40.0%
AGCAATCAAAGGACTAGAAG+AGG - chr5.2:3215644-3215663 MS.gene21647:CDS 40.0%
AGCATTGTGAGGATGCAAAA+AGG - chr5.2:3215485-3215504 MS.gene21647:CDS 40.0%
ATGAAGCCAAAGAAAGACCA+AGG - chr5.2:3214972-3214991 MS.gene21647:CDS 40.0%
ATGCTAGAGTGAATATGTCG+TGG + chr5.2:3213042-3213061 None:intergenic 40.0%
ATGCTGCCATGTTTCAGATA+TGG - chr5.2:3215556-3215575 MS.gene21647:CDS 40.0%
ATGTCACAACAATCTACAGG+TGG + chr5.2:3214591-3214610 None:intergenic 40.0%
CAAAAGTCTCAGAATCTGAG+AGG - chr5.2:3215240-3215259 MS.gene21647:CDS 40.0%
CATCTTCACAAACTGTGCTT+TGG + chr5.2:3214833-3214852 None:intergenic 40.0%
CTCAAAGGACTCAATGGAAA+TGG + chr5.2:3215614-3215633 None:intergenic 40.0%
GAGAACAAAATCTGTCTCCT+TGG + chr5.2:3213937-3213956 None:intergenic 40.0%
GAGACCAATCACCATTTAAC+AGG - chr5.2:3215381-3215400 MS.gene21647:CDS 40.0%
GATCCAAAGATTACCAAGTG+AGG - chr5.2:3213668-3213687 MS.gene21647:intron 40.0%
GCATTGTGAGGATGCAAAAA+GGG - chr5.2:3215486-3215505 MS.gene21647:CDS 40.0%
GGGTTAATCTCTTCACCAAT+TGG + chr5.2:3214180-3214199 None:intergenic 40.0%
GGTTGATCTCATGCTTCAAA+TGG - chr5.2:3213969-3213988 MS.gene21647:CDS 40.0%
GTACTAACAACAGCATTGTG+AGG - chr5.2:3215474-3215493 MS.gene21647:CDS 40.0%
GTCCATATGTGAGCAATCAA+AGG - chr5.2:3215633-3215652 MS.gene21647:CDS 40.0%
GTCCTTTGATTGCTCACATA+TGG + chr5.2:3215638-3215657 None:intergenic 40.0%
TACCAAGTGAGGAGAAGATT+GGG - chr5.2:3213679-3213698 MS.gene21647:intron 40.0%
TACCACCAACATTATCTGGT+CGG - chr5.2:3215144-3215163 MS.gene21647:CDS 40.0%
TATGACCTTGAAGGACTCAT+TGG + chr5.2:3215527-3215546 None:intergenic 40.0%
TATGGACTCAAAGGACTCAA+TGG + chr5.2:3215620-3215639 None:intergenic 40.0%
TCGGGTTTCAAGGACAAATA+AGG - chr5.2:3215163-3215182 MS.gene21647:CDS 40.0%
TGTCACAACAATCTACAGGT+GGG + chr5.2:3214590-3214609 None:intergenic 40.0%
TTACCAAGTGAGGAGAAGAT+TGG - chr5.2:3213678-3213697 MS.gene21647:intron 40.0%
TTCACCTTCTTTGAATACCC+TGG + chr5.2:3213158-3213177 None:intergenic 40.0%
TTGCTCACATATGGACTCAA+AGG + chr5.2:3215629-3215648 None:intergenic 40.0%
TTTGTTCTCTATACCTGTGG+TGG - chr5.2:3213948-3213967 MS.gene21647:CDS 40.0%
! AACTGACCTTGGTCTTTCTT+TGG + chr5.2:3214981-3215000 None:intergenic 40.0%
! CTTTCCAGGGTATTCAAAGA+AGG - chr5.2:3213151-3213170 MS.gene21647:CDS 40.0%
! GAAGCACTCAAGGAGAAAAA+AGG - chr5.2:3213505-3213524 MS.gene21647:intron 40.0%
! GACAGGGAAAATTCCTTTTG+AGG - chr5.2:3214323-3214342 MS.gene21647:CDS 40.0%
! TAGGACAAAGTTTTGCTCTG+AGG - chr5.2:3213734-3213753 MS.gene21647:CDS 40.0%
! TCAAGAGTTTTCCAATGGAG+AGG - chr5.2:3214625-3214644 MS.gene21647:CDS 40.0%
! TCTAGCTCAGTTAAAACCTC+AGG + chr5.2:3214219-3214238 None:intergenic 40.0%
! TCTCCTCACTTGGTAATCTT+TGG + chr5.2:3213674-3213693 None:intergenic 40.0%
! TCTCTAAGAGGACATTCAAG+AGG + chr5.2:3213130-3213149 None:intergenic 40.0%
! TTTGGAGGCCTTCTTTTAAC+TGG + chr5.2:3215224-3215243 None:intergenic 40.0%
!! TGGAAGTCTAAAAGCGCTTA+TGG - chr5.2:3212970-3212989 MS.gene21647:CDS 40.0%
!! TTGGAGGCCTTCTTTTAACT+GGG + chr5.2:3215223-3215242 None:intergenic 40.0%
!!! AGATATTTTGGCGAGCGATA+TGG + chr5.2:3214411-3214430 None:intergenic 40.0%
!!! CTCTCAGATTCTGAGACTTT+TGG + chr5.2:3215242-3215261 None:intergenic 40.0%
!!! TCAGATTCTGAGACTTTTGG+AGG + chr5.2:3215239-3215258 None:intergenic 40.0%
!!! TTGCTCTGAGGCATTTTCTT+GGG - chr5.2:3213746-3213765 MS.gene21647:CDS 40.0%
!!! TTTGCTCTGAGGCATTTTCT+TGG - chr5.2:3213745-3213764 MS.gene21647:CDS 40.0%
AAGTGAGGAGAAGATTGGGA+AGG - chr5.2:3213683-3213702 MS.gene21647:intron 45.0%
ACAGTAGCCACATCCTTTGT+TGG + chr5.2:3214789-3214808 None:intergenic 45.0%
ACCACCAACATTATCTGGTC+GGG - chr5.2:3215145-3215164 MS.gene21647:CDS 45.0%
AGTGAGGAGAAGATTGGGAA+GGG - chr5.2:3213684-3213703 MS.gene21647:CDS 45.0%
ATGCTTGGAGAGAAGATAGG+TGG - chr5.2:3213473-3213492 MS.gene21647:intron 45.0%
ATTGAAGCGATCGAGAATGC+TGG - chr5.2:3213298-3213317 MS.gene21647:CDS 45.0%
CATTATCTGGTCGGGTTTCA+AGG - chr5.2:3215153-3215172 MS.gene21647:CDS 45.0%
CTCAAAGGGCTCAATGCTAA+TGG + chr5.2:3215680-3215699 None:intergenic 45.0%
CTTCTGGTGTATGACCTTGA+AGG + chr5.2:3215536-3215555 None:intergenic 45.0%
GAAACCCGACCAGATAATGT+TGG + chr5.2:3215152-3215171 None:intergenic 45.0%
GCAAGCAATTGGAGAATGCT+TGG - chr5.2:3213458-3213477 MS.gene21647:intron 45.0%
GTGGAGATATTCAATGCCTC+TGG + chr5.2:3213995-3214014 None:intergenic 45.0%
TAGTGAGTCTGAGCCAACAA+AGG - chr5.2:3214773-3214792 MS.gene21647:CDS 45.0%
TCCTCTTAGAGAGCTTTCCA+GGG - chr5.2:3213138-3213157 MS.gene21647:CDS 45.0%
TGAAACATGGCAGCATCTTC+TGG + chr5.2:3215552-3215571 None:intergenic 45.0%
TGAGAGAGAATTGTGGTCCA+AGG - chr5.2:3213917-3213936 MS.gene21647:CDS 45.0%
TTCCCAATCTTCTCCTCACT+TGG + chr5.2:3213684-3213703 None:intergenic 45.0%
TTGAAGCGATCGAGAATGCT+GGG - chr5.2:3213299-3213318 MS.gene21647:CDS 45.0%
!! CTTGCTTTGCTATTTCCCTG+CGG - chr5.2:3213273-3213292 MS.gene21647:CDS 45.0%
!! TGGAGGCCTTCTTTTAACTG+GGG + chr5.2:3215222-3215241 None:intergenic 45.0%
!! TGTGACGGAGTTGATGAACA+AGG - chr5.2:3213879-3213898 MS.gene21647:CDS 45.0%
!!! TGCTCTGAGGCATTTTCTTG+GGG - chr5.2:3213747-3213766 MS.gene21647:CDS 45.0%
ACCCGACCAGATAATGTTGG+TGG + chr5.2:3215149-3215168 None:intergenic 50.0%
ACCCTGGAAAGCTCTCTAAG+AGG + chr5.2:3213142-3213161 None:intergenic 50.0%
AGAATGCTGGGGAGATGTCA+GGG - chr5.2:3213311-3213330 MS.gene21647:CDS 50.0%
AGCATGAGATCAACCACCAC+AGG + chr5.2:3213964-3213983 None:intergenic 50.0%
CAAGAACCTCACCAATGTGC+CGG + chr5.2:3212952-3212971 None:intergenic 50.0%
CGATCGCTTCAATAACCGCA+GGG + chr5.2:3213291-3213310 None:intergenic 50.0%
CTCATGCTTCAAATGGCCAG+AGG - chr5.2:3213976-3213995 MS.gene21647:CDS 50.0%
CTTGGAGAGAAGATAGGTGG+AGG - chr5.2:3213476-3213495 MS.gene21647:intron 50.0%
GACCTTGAAGGACTCATTGG+CGG + chr5.2:3215524-3215543 None:intergenic 50.0%
GGCGAGCGATATGGAAACAA+AGG + chr5.2:3214402-3214421 None:intergenic 50.0%
GGGAAGGGGGAAGGAATTTA+AGG - chr5.2:3213699-3213718 MS.gene21647:CDS 50.0%
GTCCTCTTAGAGAGCTTTCC+AGG - chr5.2:3213137-3213156 MS.gene21647:CDS 50.0%
GTCTGAGCCAACAAAGGATG+TGG - chr5.2:3214779-3214798 MS.gene21647:CDS 50.0%
GTGAGGAGAAGATTGGGAAG+GGG - chr5.2:3213685-3213704 MS.gene21647:CDS 50.0%
TCGATCGCTTCAATAACCGC+AGG + chr5.2:3213292-3213311 None:intergenic 50.0%
TGAAGCGATCGAGAATGCTG+GGG - chr5.2:3213300-3213319 MS.gene21647:CDS 50.0%
TGAGGAGAAGATTGGGAAGG+GGG - chr5.2:3213686-3213705 MS.gene21647:CDS 50.0%
!! GAGGCTCATAGAAGCACTCA+AGG - chr5.2:3213495-3213514 MS.gene21647:intron 50.0%
ATGGAACAGCTCCGGCACAT+TGG - chr5.2:3212938-3212957 MS.gene21647:CDS 55.0%
CACCGCCAATGAGTCCTTCA+AGG - chr5.2:3215519-3215538 MS.gene21647:CDS 55.0%
CGGCACATTGGTGAGGTTCT+TGG - chr5.2:3212950-3212969 MS.gene21647:CDS 55.0%
GAGAAACCAGGACGCTGAGA+CGG + chr5.2:3214468-3214487 None:intergenic 55.0%
GAGAAGATTGGGAAGGGGGA+AGG - chr5.2:3213690-3213709 MS.gene21647:CDS 55.0%
GAGAATGCTGGGGAGATGTC+AGG - chr5.2:3213310-3213329 MS.gene21647:CDS 55.0%
GGAGATGTCAGGGTTGGATC+AGG - chr5.2:3213321-3213340 MS.gene21647:CDS 55.0%
ACAGCTCCGGCACATTGGTG+AGG - chr5.2:3212943-3212962 MS.gene21647:CDS 60.0%
ACTGATCCGTCTCAGCGTCC+TGG - chr5.2:3214459-3214478 MS.gene21647:CDS 60.0%
TGCTGGGGAGATGTCAGGGT+TGG - chr5.2:3213315-3213334 MS.gene21647:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 3212938 3215739 3212938 ID=MS.gene21647
chr5.2 mRNA 3212938 3215739 3212938 ID=MS.gene21647.t1;Parent=MS.gene21647
chr5.2 exon 3213684 3215739 3213684 ID=MS.gene21647.t1.exon1;Parent=MS.gene21647.t1
chr5.2 CDS 3213684 3215739 3213684 ID=cds.MS.gene21647.t1;Parent=MS.gene21647.t1
chr5.2 exon 3212938 3213452 3212938 ID=MS.gene21647.t1.exon2;Parent=MS.gene21647.t1
chr5.2 CDS 3212938 3213452 3212938 ID=cds.MS.gene21647.t1;Parent=MS.gene21647.t1
Gene Sequence

>MS.gene21647

ATGGAACAGCTCCGGCACATTGGTGAGGTTCTTGGAAGTCTAAAAGCGCTTATGGTTTTAAGCGACGAAATTCAAATCAATCAACGACAATGTTGTTTGCTCCACGACATATTCACTCTAGCATTCGACACAATCGCAGATGAGATAAGGCAGAATCTGAAGCTTGAAGACAGAACTACAAAATGGAAGCCTCTTGAATGTCCTCTTAGAGAGCTTTCCAGGGTATTCAAAGAAGGTGAATTATACATTAAGCATTGTTTGGATTCAAAAGATTGGTCAGGGAAAGCACTCACACTTTCTCAGAACAGAGAATGTGTTGAGTTTCACATTCATAACTTGCTTTGCTATTTCCCTGCGGTTATTGAAGCGATCGAGAATGCTGGGGAGATGTCAGGGTTGGATCAGGATGAGAATTCGAAGAAGAAATTGATGCTTGCAAGAAAATATGATGTGGAATGGAATGATCCAAAACTTTTTCAGTGGAGATTTGGCAAACAATATTTGGTTCCAAAAGAAATTTGCAAGCAATTGGAGAATGCTTGGAGAGAAGATAGGTGGAGGCTCATAGAAGCACTCAAGGAGAAAAAAGGTTCTTCCAAGCTCACTTCTTCAAAGAATGATCAACATTTTGCAGATATGTTGTTGAAGAAATTGATGAATGGTAATTCGGATAAAACTAATCACAAAAACAGTAATGATAAGGTATTGTGGCCAATTAGTGTTTTGTTAGGATCCAAAGATTACCAAGTGAGGAGAAGATTGGGAAGGGGGAAGGAATTTAAGGAAATTCAATGGTTAGGACAAAGTTTTGCTCTGAGGCATTTTCTTGGGGAGAGAGAAGCTTATGAAAATGAGATTTCCAATTTGTTATCACTTTCTCACCCAAACATATTGCAATATCTATGTGGTTTTTATGATGAAGAGAAGAAAGAGTTTTCTCTTGTGACGGAGTTGATGAACAAGGATTTGTGGACATACATGAGAGAGAATTGTGGTCCAAGGAGACAGATTTTGTTCTCTATACCTGTGGTGGTTGATCTCATGCTTCAAATGGCCAGAGGCATTGAATATCTCCACTCCAAAAACATTTACCATGGAAACCTTAATCCTTGTAATATCCTACTAAAAGCTAGAAACTCACAAGAAGGTTATTTTCAAGCAAAAGTTGTTGGATTTGGATTGTCATCTGTTGAAAATGGTGAAATTTATAATGCTTCTAGATCCTCACCAACACATAATCCAATTGGTGAAGAGATTAACCCTATAATTTGGTATGCACCTGAGGTTTTAACTGAGCTAGAACAAATGAAAAATGCTTCTACCTCTTGTAAGTACTCAGAGAAAGCCGATGCATATAGCTTTGGTATGATATGTTTTGAGTTGTTGACAGGGAAAATTCCTTTTGAGGATAATCATTTTCAAGGGATTAGAACCAACCAAAATATCAAAGCAGGAGAGAGGCCTTTGTTTCCATATCGCTCGCCAAAATATCTTGTGAGTTTGATCAAAAAATGTTGGCAAACTGATCCGTCTCAGCGTCCTGGTTTCTCATCTATTTGTAGGATTTTGCGCTACATCAAAAAGTTTCTTTCAATGAACACCGAGTATGTTTTGATCAATCCTGAACTAAATCAGCTTGAACTTCAGAACCCACCTGTAGATTGTTGTGACATTGAAGGAATATTTCTCAAGAGTTTTCCAATGGAGAGGACATATAATGTGTCTTTTGTTTCACAAATACCTTATGAAATGTTTGCTTATAAAGTTGTTGAAAAAGGGAAGATTAATAATCAGAATCTGAGTAATAATACTATTCCTACTATAGATAATAAGGGTAGTGAGTCTGAGCCAACAAAGGATGTGGCTACTGTTCGTAGCGAAGAAAGTGATGACCAAAGCACAGTTTGTGAAGATGTTGATGCATCCATAATTAAAGATCTGGTAACCTATCCAAAATCAATTTCTGATGACACACAGTCTATTTTTTATGATACCCCGTCAAAGAAGAATGTTACAATAAAGAAACCATCACAAATGAAGCCAAAGAAAGACCAAGGTCAGTTTAATTTTATTATCATCTCAATTTTCACCTGCTATATATATATATATATATATATATATATATATATATATATATATATAATAAACTGATTATGTATTGAATTGATTGAAACAGGAACGCCTAAATTGCAAGCAACAAAATCATTACCACCAACATTATCTGGTCGGGTTTCAAGGACAAATAAGGCGAACCAGTAATCATTGACATCAAGTTCTATAACCCCAGTTAAAAGAAGGCCTCCAAAAGTCTCAGAATCTGAGAGGAAATCCAATATTAACAAAGGGAATCAATCAGCATCAGTGTCAACTTCAAGCTCAGTTAGAAAAAAAGTATATGGAGGCAATGTCTCAGATTCTAAGCCTAGTTTAAATCTGAAAACAAGAGACCAATCACCATTTAACAGGTTAAAGACAAAAAAATCACAACTAACGATGAATTCTTCTTCGAGTCCAACAAGAATAAGACGTGTCAATAGTACTAACAACAGCATTGTGAGGATGCAAAAAGGGTTCATTTCAACACCGCCAATGAGTCCTTCAAGGTCATACACCAGAAGATGCTGCCATGTTTCAGATATGGATAGTTCTTTGAAGATACGAGGTAGATTGTCACCATTTCCATTGAGTCCTTTGAGTCCATATGTGAGCAATCAAAGGACTAGAAGAGGAAGCTTGTCACCATTAGCATTGAGCCCTTTGAGTCCTTATGTAAGAACAACATATGGTCATGTCTCAGATTAA
Protein sequence

>MS.gene21647.t1

MEQLRHIGEVLGSLKALMVLSDEIQINQRQCCLLHDIFTLAFDTIADEIRQNLKLEDRTTKWKPLECPLRELSRVFKEGELYIKHCLDSKDWSGKALTLSQNRECVEFHIHNLLCYFPAVIEAIENAGEMSGLDQDENSKKKLMLARKYDVEWNDPKLFQWRFGKQYLVPKEICKQLENAWREDRWRLIEALKEKKGSSKLTSSKNDQHFADMLLKKLMNGNSDKTNHKNSNDKVLWPISVLLGSKDYQVRRRLGRGKEFKEIQWLGQSFALRHFLGEREAYENEISNLLSLSHPNILQYLCGFYDEEKKEFSLVTELMNKDLWTYMRENCGPRRQILFSIPVVVDLMLQMARGIEYLHSKNIYHGNLNPCNILLKARNSQEGYFQAKVVGFGLSSVENGEIYNASRSSPTHNPIGEEINPIIWYAPEVLTELEQMKNASTSCKYSEKADAYSFGMICFELLTGKIPFEDNHFQGIRTNQNIKAGERPLFPYRSPKYLVSLIKKCWQTDPSQRPGFSSICRILRYIKKFLSMNTEYVLINPELNQLELQNPPVDCCDIEGIFLKSFPMERTYNVSFVSQIPYEMFAYKVVEKGKINNQNLSNNTIPTIDNKGSESEPTKDVATVRSEESDDQSTVCEDVDASIIKDLVTYPKSISDDTQSIFYDTPSKKNVTIKKPSQMKPKKDQVKRRPPKVSESERKSNINKGNQSASVSTSSSVRKKVYGGNVSDSKPSLNLKTRDQSPFNRLKTKKSQLTMNSSSSPTRIRRVNSTNNSIVRMQKGFISTPPMSPSRSYTRRCCHVSDMDSSLKIRGRLSPFPLSPLSPYVSNQRTRRGSLSPLALSPLSPYVRTTYGHVSD