AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072869

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072869.t1 MTR_3g106690 97.598 333 8 0 1 333 59 391 0.0 680
MS.gene072869.t1 MTR_7g103260 58.559 333 135 2 1 333 68 397 2.19e-149 426
MS.gene072869.t1 MTR_1g061760 57.784 334 137 3 1 333 154 484 1.73e-144 417
MS.gene072869.t1 MTR_7g103270 59.104 335 130 4 1 333 69 398 7.69e-144 412
MS.gene072869.t1 MTR_3g080690 58.006 331 136 2 1 331 115 442 4.57e-143 412
MS.gene072869.t1 MTR_3g081240 58.308 331 135 2 1 331 117 444 4.99e-143 412
MS.gene072869.t1 MTR_3g097310 58.559 333 135 2 1 333 162 491 1.23e-140 407
MS.gene072869.t1 MTR_4g115530 53.846 338 149 3 1 333 55 390 2.65e-130 377
MS.gene072869.t1 MTR_4g115530 53.846 338 149 3 1 333 1 336 3.93e-130 375
MS.gene072869.t1 MTR_4g115540 54.142 338 148 3 1 333 289 624 1.12e-128 381
MS.gene072869.t1 MTR_4g115460 52.976 336 153 3 1 333 129 462 3.13e-124 364
MS.gene072869.t1 MTR_4g100730 47.917 336 167 5 1 330 200 533 2.99e-103 313
MS.gene072869.t1 MTR_3g110198 46.386 332 170 4 1 329 264 590 2.88e-97 300
MS.gene072869.t1 MTR_7g103150 45.482 332 173 5 1 329 155 481 3.48e-97 296
MS.gene072869.t1 MTR_3g110200 44.578 332 176 5 1 329 261 587 2.12e-96 297
MS.gene072869.t1 MTR_1g006600 45.783 332 172 5 1 329 257 583 2.19e-94 292
MS.gene072869.t1 MTR_8g102840 42.342 333 183 4 1 329 279 606 2.61e-90 282
MS.gene072869.t1 MTR_1g090733 43.413 334 175 6 1 330 222 545 9.93e-90 279
MS.gene072869.t1 MTR_4g115540 53.629 248 108 3 1 243 289 534 1.57e-89 278
MS.gene072869.t1 MTR_5g031480 39.706 340 182 8 1 332 90 414 7.28e-77 241
MS.gene072869.t1 MTR_5g049790 39.763 337 170 9 1 332 126 434 4.80e-73 232
MS.gene072869.t1 MTR_7g085840 39.706 340 181 11 1 331 1 325 1.99e-64 207
MS.gene072869.t1 MTR_8g102840 39.919 248 140 4 1 244 279 521 1.49e-57 194
MS.gene072869.t1 MTR_5g031480 39.062 256 133 8 1 248 90 330 2.98e-48 165
MS.gene072869.t1 MTR_5g031480 38.824 255 133 8 1 247 90 329 1.28e-47 164
MS.gene072869.t1 MTR_5g049790 39.437 213 109 7 1 208 126 323 1.03e-36 134
MS.gene072869.t1 MTR_5g049790 39.437 213 109 7 1 208 143 340 1.07e-36 135
MS.gene072869.t1 MTR_8g096140 28.873 284 175 7 67 324 102 384 6.49e-29 115
MS.gene072869.t1 MTR_8g096160 29.577 284 173 8 67 324 102 384 9.12e-29 115
MS.gene072869.t1 MTR_2g084245 28.000 300 192 7 45 324 131 426 5.42e-25 105
MS.gene072869.t1 MTR_4g057500 28.896 308 179 10 45 324 149 444 4.73e-24 102
MS.gene072869.t1 MTR_8g080490 25.079 315 206 12 27 324 97 398 1.97e-16 80.1
MS.gene072869.t1 MTR_3g037720 25.773 291 198 8 45 324 187 470 3.66e-16 79.7
MS.gene072869.t1 MTR_1g029350 24.924 329 206 9 2 302 71 386 4.11e-16 79.3
MS.gene072869.t1 MTR_6g027310 26.452 310 191 12 45 334 183 475 3.56e-15 76.6
MS.gene072869.t1 MTR_4g084200 25.949 316 180 13 45 333 160 448 8.90e-15 75.5
MS.gene072869.t1 MTR_4g077920 25.079 315 192 9 49 333 111 411 3.52e-14 73.6
MS.gene072869.t1 MTR_3g085480 26.263 297 190 12 45 324 116 400 1.22e-13 71.6
MS.gene072869.t1 MTR_4g084200 27.559 254 141 10 45 274 160 394 6.40e-13 69.7
MS.gene072869.t1 MTR_4g084200 27.559 254 141 10 45 274 160 394 7.24e-13 69.3
MS.gene072869.t1 MTR_4g077920 25.954 262 157 8 95 333 222 469 4.37e-12 67.0
MS.gene072869.t1 MTR_7g087100 24.055 291 202 7 45 324 188 470 5.34e-12 67.0
MS.gene072869.t1 MTR_4g077920 27.350 234 147 8 95 312 222 448 1.68e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072869.t1 AT5G25310 67.665 334 106 2 1 333 147 479 1.23e-169 481
MS.gene072869.t1 AT5G03795 58.084 334 137 2 1 334 186 516 1.03e-143 416
MS.gene072869.t1 AT3G07620 57.658 333 139 1 1 333 128 458 4.02e-137 397
MS.gene072869.t1 AT5G11130 51.796 334 158 2 1 333 1 332 1.13e-132 381
MS.gene072869.t1 AT5G11130 51.796 334 158 2 1 333 145 476 2.22e-131 383
MS.gene072869.t1 AT5G20260 52.695 334 156 2 1 333 1 333 7.94e-126 364
MS.gene072869.t1 AT5G20260 52.695 334 156 2 1 333 28 360 9.85e-126 365
MS.gene072869.t1 AT5G20260 52.695 334 156 2 1 333 133 465 4.88e-125 367
MS.gene072869.t1 AT3G42180 50.147 341 160 3 1 333 1 339 6.87e-122 354
MS.gene072869.t1 AT5G33290 51.613 341 155 3 1 333 160 498 2.14e-121 358
MS.gene072869.t1 AT3G42180 50.147 341 160 3 1 333 130 468 6.29e-121 356
MS.gene072869.t1 AT4G16745 48.955 335 165 3 1 330 196 529 7.41e-112 335
MS.gene072869.t1 AT5G19670 46.246 333 170 4 1 329 277 604 1.20e-101 311
MS.gene072869.t1 AT5G19670 46.246 333 170 4 1 329 277 604 1.20e-101 311
MS.gene072869.t1 AT4G16745 48.710 310 153 3 1 305 196 504 4.78e-101 309
MS.gene072869.t1 AT4G32790 45.345 333 173 5 1 329 263 590 1.21e-95 295
MS.gene072869.t1 AT4G32790 45.345 333 173 5 1 329 263 590 1.21e-95 295
MS.gene072869.t1 AT4G32790 45.345 333 173 5 1 329 263 590 1.21e-95 295
MS.gene072869.t1 AT5G25820 44.012 334 178 5 1 329 320 649 9.44e-91 284
MS.gene072869.t1 AT5G37000 41.246 337 180 6 1 329 222 548 4.35e-84 265
MS.gene072869.t1 AT5G11610 42.090 335 180 10 1 329 216 542 4.41e-80 254
MS.gene072869.t1 AT4G38040 40.000 340 181 8 1 332 100 424 1.46e-74 236
MS.gene072869.t1 AT4G38040 39.550 311 165 8 1 303 100 395 2.62e-63 206
MS.gene072869.t1 AT5G11610 39.834 241 132 9 1 236 216 448 1.70e-47 166
MS.gene072869.t1 AT1G27440 29.097 299 186 9 45 324 89 380 2.33e-28 114
MS.gene072869.t1 AT5G61840 28.523 298 189 8 45 324 92 383 7.47e-28 112
MS.gene072869.t1 AT5G22940 29.126 309 188 10 45 330 152 452 9.51e-27 110
MS.gene072869.t1 AT5G22940 29.126 309 188 10 45 330 181 481 1.28e-26 110
MS.gene072869.t1 AT2G28110 26.496 351 216 9 5 324 94 433 2.08e-26 108
MS.gene072869.t1 AT5G11610 41.739 115 64 2 1 113 216 329 8.47e-25 103
MS.gene072869.t1 AT1G74680 27.365 296 187 9 45 324 149 432 2.67e-15 77.0
MS.gene072869.t1 AT1G21480 26.190 294 175 13 5 270 89 368 2.94e-15 76.6
MS.gene072869.t1 AT1G21480 24.107 336 194 13 5 302 89 401 3.87e-15 76.3
MS.gene072869.t1 AT1G67410 24.841 314 212 10 27 324 101 406 2.62e-14 73.9
MS.gene072869.t1 AT2G35100 25.597 293 167 12 45 310 127 395 3.24e-14 73.6
MS.gene072869.t1 AT5G16890 28.175 252 164 8 95 333 228 475 7.74e-13 69.7
MS.gene072869.t1 AT3G45400 31.933 119 78 1 218 333 327 445 6.97e-12 66.6
MS.gene072869.t1 AT3G03650 24.735 283 186 9 45 312 193 463 6.59e-11 63.5

Find 99 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGAGACAAATTGCGTTAA+AGG 0.178188 3.2:+87463342 None:intergenic
CCTCCATAGAGATGAATTTC+TGG 0.201182 3.2:+87462791 None:intergenic
GAACTTGGAGCTTAGCATTA+TGG 0.244455 3.2:+87462592 None:intergenic
CGGTATCTTGCCTTCTTTGC+CGG 0.249948 3.2:-87462719 MS.gene072869:CDS
TAAATTGATTCCACAATTCT+TGG 0.255386 3.2:+87462536 None:intergenic
AAACCCCATGTTCCATTTCA+TGG 0.279328 3.2:+87463472 None:intergenic
TTACCAGAAATTCATCTCTA+TGG 0.286433 3.2:-87462794 MS.gene072869:CDS
GAATAGTAGTCTATGCCTTT+AGG 0.293641 3.2:+87462614 None:intergenic
TCATGCTTGCTTGCCATGAT+TGG 0.306171 3.2:-87463257 MS.gene072869:CDS
TATCAATGAGGTACCCCTTC+TGG 0.307231 3.2:-87463305 MS.gene072869:CDS
GGTAAAGATACATCCTTTAG+AGG 0.312438 3.2:+87462812 None:intergenic
TTCCAGTGTTGGAGAAGAAT+TGG 0.325056 3.2:+87462674 None:intergenic
TGGAGAAGAATTGGGCGAAT+TGG 0.350998 3.2:+87462683 None:intergenic
TACCGGCGTGGGGCGTTGTC+AGG 0.370199 3.2:+87462737 None:intergenic
GGTGGAAGTGAAAGGAGTTT+AGG 0.373477 3.2:+87462758 None:intergenic
GGAGAAGAATTGGGCGAATT+GGG 0.381140 3.2:+87462684 None:intergenic
AATAGTAGTCTATGCCTTTA+GGG 0.390854 3.2:+87462615 None:intergenic
AGAAGCTCAAGCAAGGAGTA+AGG 0.412812 3.2:-87462364 MS.gene072869:CDS
ATGAAATGGAACATGGGGTT+TGG 0.415076 3.2:-87463470 MS.gene072869:CDS
TCCAGTGTTGGAGAAGAATT+GGG 0.427997 3.2:+87462675 None:intergenic
AGCTTCTTATACTCACTCTC+TGG 0.429763 3.2:+87462380 None:intergenic
TGAAGGTTTCAATCCTCTAA+AGG 0.433578 3.2:-87462825 MS.gene072869:CDS
AAATTGATTCCACAATTCTT+GGG 0.433600 3.2:+87462537 None:intergenic
AAAGTAAACATGAGCTGCAT+TGG 0.434974 3.2:+87463428 None:intergenic
AGGAAGGTTCCTCCATGAAA+TGG 0.439602 3.2:-87463484 MS.gene072869:CDS
GCCCAATTCTTCTCCAACAC+TGG 0.442592 3.2:-87462676 MS.gene072869:CDS
CATGCTTGCTTGCCATGATT+GGG 0.450735 3.2:-87463256 MS.gene072869:intron
ACAATAGGTAGGTCCCCATC+TGG 0.451801 3.2:+87463543 None:intergenic
TAAAGGAGTGACGTCGTAAG+AGG 0.467103 3.2:+87463359 None:intergenic
TGTTCATGATGGCCCGTGTA+AGG 0.468004 3.2:-87463523 MS.gene072869:CDS
TGGATGTTTCAGATATTCCT+AGG 0.469967 3.2:-87462427 MS.gene072869:CDS
CACGGGCCATCATGAACAAT+AGG 0.471146 3.2:+87463528 None:intergenic
TTCTTTGCCGGTGGTATGCA+TGG 0.475267 3.2:-87462707 MS.gene072869:CDS
CCTTCAGTGATGTGCTGCAA+TGG 0.481513 3.2:-87462469 MS.gene072869:CDS
ATGGAGGAACCTTCCTTCGA+TGG 0.484390 3.2:+87463491 None:intergenic
ACTTGGACAAAGACAGAACT+TGG 0.485720 3.2:+87462577 None:intergenic
TGCAATGCCAACACTTCTGA+AGG 0.485933 3.2:-87462842 MS.gene072869:CDS
AAGTAAACATGAGCTGCATT+GGG 0.487410 3.2:+87463429 None:intergenic
TTGAACAAATTACCCAATCA+TGG 0.495105 3.2:+87463244 None:intergenic
ATGAGCTGCATTGGGATCAC+TGG 0.499158 3.2:+87463437 None:intergenic
ACCATCCATGTCACACTGAA+TGG 0.500488 3.2:+87463402 None:intergenic
CTTCAGTGATGTGCTGCAAT+GGG 0.500866 3.2:-87462468 MS.gene072869:CDS
CGGCGTGGGGCGTTGTCAGG+TGG 0.505683 3.2:+87462740 None:intergenic
GCAGGGTCCACATGCATCAA+AGG 0.511644 3.2:-87462903 MS.gene072869:intron
ATGCATACCACCGGCAAAGA+AGG 0.518456 3.2:+87462709 None:intergenic
GTCAATGAGTATCTCCCTAA+AGG 0.518478 3.2:-87462629 MS.gene072869:CDS
AAGTTCTGTCTTTGTCCAAG+TGG 0.519604 3.2:-87462575 MS.gene072869:CDS
GAAGCTCAAGCAAGGAGTAA+GGG 0.521382 3.2:-87462363 MS.gene072869:CDS
CGTAAGAGGATGGTGTATAG+AGG 0.521550 3.2:+87463373 None:intergenic
ATTGAAACCTTCAGAAGTGT+TGG 0.527913 3.2:+87462835 None:intergenic
CTAAGAATCTCTTTCAACCT+AGG 0.532971 3.2:+87462410 None:intergenic
CTAGCCACTTCAAAACCACT+TGG 0.537494 3.2:+87462560 None:intergenic
AAGGTGTATGTATACCCAGA+TGG 0.543645 3.2:-87463558 MS.gene072869:CDS
GGAGATGGAGAAGATATTCA+AGG 0.549467 3.2:-87463577 None:intergenic
CAGGGTCCACATGCATCAAA+GGG 0.550516 3.2:-87462902 MS.gene072869:CDS
CGTTGTCAGGTGGAAGTGAA+AGG 0.555053 3.2:+87462750 None:intergenic
CCATTGCAGCACATCACTGA+AGG 0.558494 3.2:+87462469 None:intergenic
TTCCTCCATGAAATGGAACA+TGG 0.562897 3.2:-87463477 MS.gene072869:CDS
CCCTTCTGGAACAGAACTCA+TGG 0.563069 3.2:-87463291 MS.gene072869:CDS
CGGCAAAGAAGGCAAGATAC+CGG 0.565879 3.2:+87462720 None:intergenic
AGGTGTATGTATACCCAGAT+GGG 0.567201 3.2:-87463557 MS.gene072869:CDS
TAATAATTTGATAGAATCAC+AGG 0.575809 3.2:+87462500 None:intergenic
AATTGGGCCATGCATACCAC+CGG 0.576027 3.2:+87462700 None:intergenic
GACCTACCTATTGTTCATGA+TGG 0.577284 3.2:-87463534 MS.gene072869:CDS
AGTGGCTAGCCCAAGAATTG+TGG 0.578684 3.2:-87462546 MS.gene072869:CDS
TCCTCCATGAAATGGAACAT+GGG 0.584529 3.2:-87463476 MS.gene072869:CDS
CCATGAGTTCTGTTCCAGAA+GGG 0.585895 3.2:+87463291 None:intergenic
ACCATGAGTTCTGTTCCAGA+AGG 0.586634 3.2:+87463290 None:intergenic
TTCTACCATTCAGTGTGACA+TGG 0.586958 3.2:-87463407 MS.gene072869:CDS
ATGGAGGTATTGTAGAGGAA+AGG 0.590050 3.2:+87462875 None:intergenic
CCAGAAATTCATCTCTATGG+AGG 0.596875 3.2:-87462791 MS.gene072869:CDS
TATCTTGCCTTCTTTGCCGG+TGG 0.597223 3.2:-87462716 MS.gene072869:CDS
GAGTATAAGAAGCTCAAGCA+AGG 0.597781 3.2:-87462371 MS.gene072869:CDS
AGAAGGCAAGATACCGGCGT+GGG 0.600970 3.2:+87462726 None:intergenic
CAATAGGTAGGTCCCCATCT+GGG 0.602453 3.2:+87463544 None:intergenic
CATGAGTTCTGTTCCAGAAG+GGG 0.604621 3.2:+87463292 None:intergenic
CACCTGACAACGCCCCACGC+CGG 0.616489 3.2:-87462739 MS.gene072869:CDS
ACCATTCAGTGTGACATGGA+TGG 0.616761 3.2:-87463403 MS.gene072869:CDS
CCTCCATGAAATGGAACATG+GGG 0.617032 3.2:-87463475 MS.gene072869:CDS
ACATATACTCCATCGAAGGA+AGG 0.623622 3.2:-87463500 MS.gene072869:CDS
GGAGTGACGTCGTAAGAGGA+TGG 0.626224 3.2:+87463363 None:intergenic
CATGGCAAGCAAGCATGAAG+TGG 0.628450 3.2:+87463262 None:intergenic
CACGAATGGAGGTATTGTAG+AGG 0.630731 3.2:+87462870 None:intergenic
ATGGAGTATATGTCCTTACA+CGG 0.631773 3.2:+87463510 None:intergenic
CATTGCAGCACATCACTGAA+GGG 0.633240 3.2:+87462470 None:intergenic
AGCAAGGAGTAAGGGCTGTG+AGG 0.634342 3.2:-87462355 MS.gene072869:CDS
CAGTGATGTGCTGCAATGGG+AGG 0.641289 3.2:-87462465 MS.gene072869:CDS
GGCATTGCACAAGACACGAA+TGG 0.644582 3.2:+87462856 None:intergenic
TGTCTCTGATTATGTGAGAG+TGG 0.651370 3.2:-87463328 MS.gene072869:CDS
GGATTGCCCTTTGATGCATG+TGG 0.662943 3.2:+87462896 None:intergenic
GAAGGCAAGATACCGGCGTG+GGG 0.662978 3.2:+87462727 None:intergenic
AAGGACATATACTCCATCGA+AGG 0.665739 3.2:-87463504 MS.gene072869:CDS
AAGAAGGCAAGATACCGGCG+TGG 0.688297 3.2:+87462725 None:intergenic
TGGAGTATATGTCCTTACAC+GGG 0.689264 3.2:+87463511 None:intergenic
CCCCATGTTCCATTTCATGG+AGG 0.689291 3.2:+87463475 None:intergenic
ATGTGAGAGTGGTATCAATG+AGG 0.692550 3.2:-87463317 MS.gene072869:CDS
GGCCATCATGAACAATAGGT+AGG 0.694574 3.2:+87463532 None:intergenic
GGTGTATGTATACCCAGATG+GGG 0.701214 3.2:-87463556 MS.gene072869:CDS
ATTGCACAAGACACGAATGG+AGG 0.708664 3.2:+87462859 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATTTAAAGGAAACAAA+AGG + chr3.2:87462906-87462925 None:intergenic 15.0%
!! AACATACAAAAACTATAGAT+TGG + chr3.2:87462737-87462756 None:intergenic 20.0%
!! AAGTGAAACTATTCATAAAA+TGG + chr3.2:87462645-87462664 None:intergenic 20.0%
!! TAATAATTTGATAGAATCAC+AGG + chr3.2:87463332-87463351 None:intergenic 20.0%
! ATTATTTACTTGACCTAAAG+TGG - chr3.2:87462861-87462880 MS.gene072869:CDS 25.0%
! GAAACTATTCATAAAATGGA+TGG + chr3.2:87462641-87462660 None:intergenic 25.0%
! GTTTCCTTTAAATATATGCA+GGG - chr3.2:87462909-87462928 MS.gene072869:intron 25.0%
! TGTTTCCTTTAAATATATGC+AGG - chr3.2:87462908-87462927 MS.gene072869:intron 25.0%
! TTACTGTACTAACAAAATGT+TGG + chr3.2:87462760-87462779 None:intergenic 25.0%
!! AAAACATCAAATCTTTTAGC+CGG + chr3.2:87463521-87463540 None:intergenic 25.0%
!! AAATTGATTCCACAATTCTT+GGG + chr3.2:87463295-87463314 None:intergenic 25.0%
!! TAAATTGATTCCACAATTCT+TGG + chr3.2:87463296-87463315 None:intergenic 25.0%
AAAAGGCTTGAAATTCACAT+TGG + chr3.2:87462889-87462908 None:intergenic 30.0%
AAAGGCTTGAAATTCACATT+GGG + chr3.2:87462888-87462907 None:intergenic 30.0%
AACTAAAATGTGGGTAAAGT+TGG + chr3.2:87462692-87462711 None:intergenic 30.0%
AATAGTAGTCTATGCCTTTA+GGG + chr3.2:87463217-87463236 None:intergenic 30.0%
ATATATGTGAAGTGTGGAAA+AGG + chr3.2:87462614-87462633 None:intergenic 30.0%
TATATGTGAAGTGTGGAAAA+GGG + chr3.2:87462613-87462632 None:intergenic 30.0%
TTACCAGAAATTCATCTCTA+TGG - chr3.2:87463035-87463054 MS.gene072869:intron 30.0%
TTGAACAAATTACCCAATCA+TGG + chr3.2:87462588-87462607 None:intergenic 30.0%
! ACATGATTTTGCACTCAATA+TGG - chr3.2:87463543-87463562 MS.gene072869:CDS 30.0%
!! TTTAGTTCTCCCAGTTATTA+GGG - chr3.2:87462705-87462724 MS.gene072869:CDS 30.0%
!!! TTTTAGTTCTCCCAGTTATT+AGG - chr3.2:87462704-87462723 MS.gene072869:CDS 30.0%
AAAGTAAACATGAGCTGCAT+TGG + chr3.2:87462404-87462423 None:intergenic 35.0%
AACTGGGAGAACTAAAATGT+GGG + chr3.2:87462701-87462720 None:intergenic 35.0%
AAGTAAACATGAGCTGCATT+GGG + chr3.2:87462403-87462422 None:intergenic 35.0%
ATGGAGTATATGTCCTTACA+CGG + chr3.2:87462322-87462341 None:intergenic 35.0%
CTAAGAATCTCTTTCAACCT+AGG + chr3.2:87463422-87463441 None:intergenic 35.0%
GAATAGTAGTCTATGCCTTT+AGG + chr3.2:87463218-87463237 None:intergenic 35.0%
GGAACAATATATGTGAAGTG+TGG + chr3.2:87462620-87462639 None:intergenic 35.0%
GGTAAAGATACATCCTTTAG+AGG + chr3.2:87463020-87463039 None:intergenic 35.0%
TAACTGGGAGAACTAAAATG+TGG + chr3.2:87462702-87462721 None:intergenic 35.0%
TCAGAGACAAATTGCGTTAA+AGG + chr3.2:87462490-87462509 None:intergenic 35.0%
TGAAGGTTTCAATCCTCTAA+AGG - chr3.2:87463004-87463023 MS.gene072869:intron 35.0%
TGGACCCTGCATATATTTAA+AGG + chr3.2:87462916-87462935 None:intergenic 35.0%
TGGATGTTTCAGATATTCCT+AGG - chr3.2:87463402-87463421 MS.gene072869:CDS 35.0%
! ACATTTTACTCTGAATCAGC+CGG - chr3.2:87463499-87463518 MS.gene072869:CDS 35.0%
! ATTGAAACCTTCAGAAGTGT+TGG + chr3.2:87462997-87463016 None:intergenic 35.0%
AAACCCCATGTTCCATTTCA+TGG + chr3.2:87462360-87462379 None:intergenic 40.0%
AAGGACATATACTCCATCGA+AGG - chr3.2:87462325-87462344 MS.gene072869:CDS 40.0%
AAGGTGTATGTATACCCAGA+TGG - chr3.2:87462271-87462290 MS.gene072869:CDS 40.0%
AAGTTCTGTCTTTGTCCAAG+TGG - chr3.2:87463254-87463273 MS.gene072869:intron 40.0%
ACATATACTCCATCGAAGGA+AGG - chr3.2:87462329-87462348 MS.gene072869:CDS 40.0%
ACTTGGACAAAGACAGAACT+TGG + chr3.2:87463255-87463274 None:intergenic 40.0%
AGCTTCTTATACTCACTCTC+TGG + chr3.2:87463452-87463471 None:intergenic 40.0%
AGGTGTATGTATACCCAGAT+GGG - chr3.2:87462272-87462291 MS.gene072869:CDS 40.0%
ATGAAATGGAACATGGGGTT+TGG - chr3.2:87462359-87462378 MS.gene072869:CDS 40.0%
ATGTGAGAGTGGTATCAATG+AGG - chr3.2:87462512-87462531 MS.gene072869:CDS 40.0%
ATTCACATTGGGACCACTTT+AGG + chr3.2:87462877-87462896 None:intergenic 40.0%
CCAGAAATTCATCTCTATGG+AGG - chr3.2:87463038-87463057 MS.gene072869:intron 40.0%
CCTCCATAGAGATGAATTTC+TGG + chr3.2:87463041-87463060 None:intergenic 40.0%
GAACTTGGAGCTTAGCATTA+TGG + chr3.2:87463240-87463259 None:intergenic 40.0%
GACCTACCTATTGTTCATGA+TGG - chr3.2:87462295-87462314 MS.gene072869:CDS 40.0%
GAGTATAAGAAGCTCAAGCA+AGG - chr3.2:87463458-87463477 MS.gene072869:CDS 40.0%
GTCAATGAGTATCTCCCTAA+AGG - chr3.2:87463200-87463219 MS.gene072869:intron 40.0%
TCCTCCATGAAATGGAACAT+GGG - chr3.2:87462353-87462372 MS.gene072869:CDS 40.0%
TCTCCCAGTTATTAGGGATT+TGG - chr3.2:87462711-87462730 MS.gene072869:CDS 40.0%
TGGAGTATATGTCCTTACAC+GGG + chr3.2:87462321-87462340 None:intergenic 40.0%
TGGCCAAATCCCTAATAACT+GGG + chr3.2:87462717-87462736 None:intergenic 40.0%
TGTCTCTGATTATGTGAGAG+TGG - chr3.2:87462501-87462520 MS.gene072869:CDS 40.0%
TTCCTCCATGAAATGGAACA+TGG - chr3.2:87462352-87462371 MS.gene072869:CDS 40.0%
TTCTACCATTCAGTGTGACA+TGG - chr3.2:87462422-87462441 MS.gene072869:CDS 40.0%
TTGGCCAAATCCCTAATAAC+TGG + chr3.2:87462718-87462737 None:intergenic 40.0%
! TCCAGTGTTGGAGAAGAATT+GGG + chr3.2:87463157-87463176 None:intergenic 40.0%
! TTCCAGTGTTGGAGAAGAAT+TGG + chr3.2:87463158-87463177 None:intergenic 40.0%
!! ATGGAGGTATTGTAGAGGAA+AGG + chr3.2:87462957-87462976 None:intergenic 40.0%
!! TGTCTCTGTTTTTCCAGTGT+TGG + chr3.2:87463169-87463188 None:intergenic 40.0%
!!! TTGTCCAAGTGGTTTTGAAG+TGG - chr3.2:87463265-87463284 MS.gene072869:CDS 40.0%
ACCATCCATGTCACACTGAA+TGG + chr3.2:87462430-87462449 None:intergenic 45.0%
ACCATGAGTTCTGTTCCAGA+AGG + chr3.2:87462542-87462561 None:intergenic 45.0%
ACCATTCAGTGTGACATGGA+TGG - chr3.2:87462426-87462445 MS.gene072869:CDS 45.0%
AGAAGCTCAAGCAAGGAGTA+AGG - chr3.2:87463465-87463484 MS.gene072869:CDS 45.0%
AGGAAGGTTCCTCCATGAAA+TGG - chr3.2:87462345-87462364 MS.gene072869:CDS 45.0%
ATTGCACAAGACACGAATGG+AGG + chr3.2:87462973-87462992 None:intergenic 45.0%
CATGAGTTCTGTTCCAGAAG+GGG + chr3.2:87462540-87462559 None:intergenic 45.0%
CATGCTTGCTTGCCATGATT+GGG - chr3.2:87462573-87462592 MS.gene072869:CDS 45.0%
CATTGCAGCACATCACTGAA+GGG + chr3.2:87463362-87463381 None:intergenic 45.0%
CCATGAGTTCTGTTCCAGAA+GGG + chr3.2:87462541-87462560 None:intergenic 45.0%
CCTCCATGAAATGGAACATG+GGG - chr3.2:87462354-87462373 MS.gene072869:CDS 45.0%
CGTAAGAGGATGGTGTATAG+AGG + chr3.2:87462459-87462478 None:intergenic 45.0%
CTAGCCACTTCAAAACCACT+TGG + chr3.2:87463272-87463291 None:intergenic 45.0%
CTTCAGTGATGTGCTGCAAT+GGG - chr3.2:87463361-87463380 MS.gene072869:CDS 45.0%
GAAGCTCAAGCAAGGAGTAA+GGG - chr3.2:87463466-87463485 MS.gene072869:CDS 45.0%
GGAGAAGAATTGGGCGAATT+GGG + chr3.2:87463148-87463167 None:intergenic 45.0%
GGCCATCATGAACAATAGGT+AGG + chr3.2:87462300-87462319 None:intergenic 45.0%
GGTGGAAGTGAAAGGAGTTT+AGG + chr3.2:87463074-87463093 None:intergenic 45.0%
GGTGTATGTATACCCAGATG+GGG - chr3.2:87462273-87462292 MS.gene072869:CDS 45.0%
TAAAGGAGTGACGTCGTAAG+AGG + chr3.2:87462473-87462492 None:intergenic 45.0%
TCATGCTTGCTTGCCATGAT+TGG - chr3.2:87462572-87462591 MS.gene072869:CDS 45.0%
TGCAATGCCAACACTTCTGA+AGG - chr3.2:87462987-87463006 MS.gene072869:intron 45.0%
TGGAGAAGAATTGGGCGAAT+TGG + chr3.2:87463149-87463168 None:intergenic 45.0%
! TATCAATGAGGTACCCCTTC+TGG - chr3.2:87462524-87462543 MS.gene072869:CDS 45.0%
!! CACGAATGGAGGTATTGTAG+AGG + chr3.2:87462962-87462981 None:intergenic 45.0%
AATTGGGCCATGCATACCAC+CGG + chr3.2:87463132-87463151 None:intergenic 50.0%
ACAATAGGTAGGTCCCCATC+TGG + chr3.2:87462289-87462308 None:intergenic 50.0%
ATGAGCTGCATTGGGATCAC+TGG + chr3.2:87462395-87462414 None:intergenic 50.0%
ATGCATACCACCGGCAAAGA+AGG + chr3.2:87463123-87463142 None:intergenic 50.0%
ATGGAGGAACCTTCCTTCGA+TGG + chr3.2:87462341-87462360 None:intergenic 50.0%
CAATAGGTAGGTCCCCATCT+GGG + chr3.2:87462288-87462307 None:intergenic 50.0%
CACGGGCCATCATGAACAAT+AGG + chr3.2:87462304-87462323 None:intergenic 50.0%
CAGGGTCCACATGCATCAAA+GGG - chr3.2:87462927-87462946 MS.gene072869:intron 50.0%
CATGGCAAGCAAGCATGAAG+TGG + chr3.2:87462570-87462589 None:intergenic 50.0%
CCATTGCAGCACATCACTGA+AGG + chr3.2:87463363-87463382 None:intergenic 50.0%
CCCCATGTTCCATTTCATGG+AGG + chr3.2:87462357-87462376 None:intergenic 50.0%
CCTTCAGTGATGTGCTGCAA+TGG - chr3.2:87463360-87463379 MS.gene072869:CDS 50.0%
CGGCAAAGAAGGCAAGATAC+CGG + chr3.2:87463112-87463131 None:intergenic 50.0%
CGGTATCTTGCCTTCTTTGC+CGG - chr3.2:87463110-87463129 MS.gene072869:intron 50.0%
CGTTGTCAGGTGGAAGTGAA+AGG + chr3.2:87463082-87463101 None:intergenic 50.0%
GCCCAATTCTTCTCCAACAC+TGG - chr3.2:87463153-87463172 MS.gene072869:intron 50.0%
GGATTGCCCTTTGATGCATG+TGG + chr3.2:87462936-87462955 None:intergenic 50.0%
GGCATTGCACAAGACACGAA+TGG + chr3.2:87462976-87462995 None:intergenic 50.0%
TATCTTGCCTTCTTTGCCGG+TGG - chr3.2:87463113-87463132 MS.gene072869:intron 50.0%
TGTTCATGATGGCCCGTGTA+AGG - chr3.2:87462306-87462325 MS.gene072869:CDS 50.0%
TTCTTTGCCGGTGGTATGCA+TGG - chr3.2:87463122-87463141 MS.gene072869:intron 50.0%
! AGTGGCTAGCCCAAGAATTG+TGG - chr3.2:87463283-87463302 MS.gene072869:CDS 50.0%
!! CCCTTCTGGAACAGAACTCA+TGG - chr3.2:87462538-87462557 MS.gene072869:CDS 50.0%
!!! ATGGGAGGCATTTTCAGTGC+AGG - chr3.2:87463379-87463398 MS.gene072869:CDS 50.0%
AAGAAGGCAAGATACCGGCG+TGG + chr3.2:87463107-87463126 None:intergenic 55.0%
AGAAGGCAAGATACCGGCGT+GGG + chr3.2:87463106-87463125 None:intergenic 55.0%
AGCAAGGAGTAAGGGCTGTG+AGG - chr3.2:87463474-87463493 MS.gene072869:CDS 55.0%
CAGTGATGTGCTGCAATGGG+AGG - chr3.2:87463364-87463383 MS.gene072869:CDS 55.0%
GCAGGGTCCACATGCATCAA+AGG - chr3.2:87462926-87462945 MS.gene072869:intron 55.0%
GGAGTGACGTCGTAAGAGGA+TGG + chr3.2:87462469-87462488 None:intergenic 55.0%
!!! GGAGGCATTTTCAGTGCAGG+TGG - chr3.2:87463382-87463401 MS.gene072869:CDS 55.0%
GAAGGCAAGATACCGGCGTG+GGG + chr3.2:87463105-87463124 None:intergenic 60.0%
CACCTGACAACGCCCCACGC+CGG - chr3.2:87463090-87463109 MS.gene072869:intron 70.0%
!! TACCGGCGTGGGGCGTTGTC+AGG + chr3.2:87463095-87463114 None:intergenic 70.0%
!! CGGCGTGGGGCGTTGTCAGG+TGG + chr3.2:87463092-87463111 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 87462256 87463595 87462256 ID=MS.gene072869
chr3.2 mRNA 87462256 87463595 87462256 ID=MS.gene072869.t1;Parent=MS.gene072869
chr3.2 exon 87463257 87463595 87463257 ID=MS.gene072869.t1.exon1;Parent=MS.gene072869.t1
chr3.2 CDS 87463257 87463595 87463257 ID=cds.MS.gene072869.t1;Parent=MS.gene072869.t1
chr3.2 exon 87462256 87462921 87462256 ID=MS.gene072869.t1.exon2;Parent=MS.gene072869.t1
chr3.2 CDS 87462256 87462921 87462256 ID=cds.MS.gene072869.t1;Parent=MS.gene072869.t1
Gene Sequence

>MS.gene072869

ATGGAGAAGATATTCAAGGTGTATGTATACCCAGATGGGGACCTACCTATTGTTCATGATGGCCCGTGTAAGGACATATACTCCATCGAAGGAAGGTTCCTCCATGAAATGGAACATGGGGTTTGGAAGTTTAGAACCAGTGATCCCAATGCAGCTCATGTTTACTTTCTACCATTCAGTGTGACATGGATGGTCAAATACCTCTATACACCATCCTCTTACGACGTCACTCCTTTAACGCAATTTGTCTCTGATTATGTGAGAGTGGTATCAATGAGGTACCCCTTCTGGAACAGAACTCATGGTGCTGACCACTTCATGCTTGCTTGCCATGATTGGGTAATTTGTTCAATTCCCTTTTCCACACTTCACATATATTGTTCCATCCATTTTATGAATAGTTTCACTTTTTGTTAATGACACACAATTGATGCCAACTTTACCCACATTTTAGTTCTCCCAGTTATTAGGGATTTGGCCAATCTATAGTTTTTGTATGTTCCAACATTTTGTTAGTACAGTAAAAAAATTAATAGTTGATATTGTAACATTTGTTATGCATATACATTTAATATCAATTATTGATTTATTTATCAAACTTTTATATTATTTACTTGACCTAAAGTGGTCCCAATGTGAATTTCAAGCCTTTTGTTTCCTTTAAATATATGCAGGGTCCACATGCATCAAAGGGCAATCCTTTCCTCTACAATACCTCCATTCGTGTCTTGTGCAATGCCAACACTTCTGAAGGTTTCAATCCTCTAAAGGATGTATCTTTACCAGAAATTCATCTCTATGGAGGTGAAGTATCTCCTAAACTCCTTTCACTTCCACCTGACAACGCCCCACGCCGGTATCTTGCCTTCTTTGCCGGTGGTATGCATGGCCCAATTCGCCCAATTCTTCTCCAACACTGGAAAAACAGAGACAAAGATATTATTGTCAATGAGTATCTCCCTAAAGGCATAGACTACTATTCCATAATGCTAAGCTCCAAGTTCTGTCTTTGTCCAAGTGGTTTTGAAGTGGCTAGCCCAAGAATTGTGGAATCAATTTATGCAGAATGTGTACCTGTGATTCTATCAAATTATTATGTGTTGCCCTTCAGTGATGTGCTGCAATGGGAGGCATTTTCAGTGCAGGTGGATGTTTCAGATATTCCTAGGTTGAAAGAGATTCTTAGTGCTATTCCAGAGAGTGAGTATAAGAAGCTCAAGCAAGGAGTAAGGGCTGTGAGGAGACATTTTACTCTGAATCAGCCGGCTAAAAGATTTGATGTTTTTCACATGATTTTGCACTCAATATGGCTAAGAAGATTGAATATAAAACTTGAATAG
Protein sequence

>MS.gene072869.t1

MEKIFKVYVYPDGDLPIVHDGPCKDIYSIEGRFLHEMEHGVWKFRTSDPNAAHVYFLPFSVTWMVKYLYTPSSYDVTPLTQFVSDYVRVVSMRYPFWNRTHGADHFMLACHDWGPHASKGNPFLYNTSIRVLCNANTSEGFNPLKDVSLPEIHLYGGEVSPKLLSLPPDNAPRRYLAFFAGGMHGPIRPILLQHWKNRDKDIIVNEYLPKGIDYYSIMLSSKFCLCPSGFEVASPRIVESIYAECVPVILSNYYVLPFSDVLQWEAFSVQVDVSDIPRLKEILSAIPESEYKKLKQGVRAVRRHFTLNQPAKRFDVFHMILHSIWLRRLNIKLE