AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene073487

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073487.t1 MTR_2g438150 98.733 789 10 0 1 789 1 789 0.0 1599
MS.gene073487.t1 MTR_2g438150 98.352 789 13 0 1 789 1 789 0.0 1594
MS.gene073487.t1 MTR_4g094660 81.337 793 138 5 1 789 1 787 0.0 1285
MS.gene073487.t1 MTR_2g438160 96.284 619 23 0 1 619 1 619 0.0 1217
MS.gene073487.t1 MTR_4g094660 80.066 607 111 5 187 789 1 601 0.0 951
MS.gene073487.t1 MTR_6g033165 49.150 765 360 6 50 789 59 819 0.0 746
MS.gene073487.t1 MTR_5g034500 48.601 786 376 9 28 789 1 782 0.0 728
MS.gene073487.t1 MTR_6g007697 48.158 787 372 11 28 789 1 776 0.0 721
MS.gene073487.t1 MTR_5g071860 45.939 751 389 4 42 789 18 754 0.0 712
MS.gene073487.t1 MTR_3g094090 46.241 798 389 8 28 789 1 794 0.0 701
MS.gene073487.t1 MTR_8g063840 46.718 777 394 8 26 789 71 840 0.0 696
MS.gene073487.t1 MTR_7g108480 44.720 767 387 6 23 787 40 771 0.0 691
MS.gene073487.t1 MTR_4g099260 44.221 796 420 10 11 789 27 815 0.0 660
MS.gene073487.t1 MTR_2g008820 43.742 791 422 10 19 789 62 849 0.0 654
MS.gene073487.t1 MTR_8g063900 43.056 792 372 10 9 787 18 743 0.0 651
MS.gene073487.t1 MTR_5g071630 43.952 744 386 8 49 789 10 725 0.0 647
MS.gene073487.t1 MTR_5g071827 45.147 680 359 3 110 789 1 666 0.0 642
MS.gene073487.t1 MTR_8g022130 42.583 782 414 7 9 789 19 766 0.0 635
MS.gene073487.t1 MTR_8g099090 43.167 783 403 10 41 789 18 792 0.0 630
MS.gene073487.t1 MTR_8g088200 43.170 776 415 8 31 789 16 782 0.0 628
MS.gene073487.t1 MTR_5g070670 43.593 757 398 10 52 789 103 849 0.0 624
MS.gene073487.t1 MTR_8g107510 38.768 779 433 11 40 786 13 779 0.0 540
MS.gene073487.t1 MTR_3g094090 45.349 602 296 4 219 789 10 609 2.34e-178 525
MS.gene073487.t1 MTR_5g034590 53.216 171 80 0 447 617 89 259 1.00e-58 204
MS.gene073487.t1 MTR_5g034590 41.860 86 45 1 109 189 5 90 6.22e-14 74.7
MS.gene073487.t1 MTR_8g022160 48.500 200 99 2 9 207 19 215 7.47e-55 190
MS.gene073487.t1 MTR_2g438170 90.909 77 7 0 650 726 1 77 2.54e-43 151
MS.gene073487.t1 MTR_1g029310 35.714 126 74 2 197 322 12 130 1.01e-18 83.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073487.t1 AT1G31120 76.336 786 182 4 6 789 13 796 0.0 1212
MS.gene073487.t1 AT2G35060 76.493 787 175 7 6 789 13 792 0.0 1208
MS.gene073487.t1 AT2G35060 76.493 787 175 7 6 789 13 792 0.0 1208
MS.gene073487.t1 AT2G35060 76.493 787 175 7 6 789 13 792 0.0 1208
MS.gene073487.t1 AT2G35060 76.396 788 175 8 6 789 13 793 0.0 1204
MS.gene073487.t1 AT4G19960 69.912 791 226 4 10 789 18 807 0.0 1078
MS.gene073487.t1 AT4G19960 68.525 807 226 5 10 789 18 823 0.0 1069
MS.gene073487.t1 AT4G19960 67.687 817 226 5 10 789 18 833 0.0 1065
MS.gene073487.t1 AT4G19960 68.954 583 169 4 218 789 5 586 0.0 795
MS.gene073487.t1 AT3G02050 49.482 772 350 6 50 789 26 789 0.0 716
MS.gene073487.t1 AT2G40540 47.229 794 387 7 28 789 1 794 0.0 708
MS.gene073487.t1 AT2G40540 47.229 794 387 7 28 789 1 794 0.0 708
MS.gene073487.t1 AT2G40540 47.229 794 387 7 28 789 1 794 0.0 708
MS.gene073487.t1 AT2G40540 47.229 794 387 7 28 789 1 794 0.0 708
MS.gene073487.t1 AT2G40540 47.229 794 387 7 28 789 1 794 0.0 708
MS.gene073487.t1 AT5G14880 49.428 787 367 11 28 789 1 781 0.0 704
MS.gene073487.t1 AT1G60160 45.511 802 413 9 3 789 35 827 0.0 702
MS.gene073487.t1 AT1G70300 47.141 787 386 9 28 789 1 782 0.0 696
MS.gene073487.t1 AT2G30070 45.140 751 353 3 42 789 18 712 0.0 669
MS.gene073487.t1 AT4G13420 44.233 789 415 9 8 787 11 783 0.0 668
MS.gene073487.t1 AT4G33530 44.760 811 408 13 5 789 59 855 0.0 663
MS.gene073487.t1 AT4G23640 44.258 775 404 7 40 789 4 775 0.0 633
MS.gene073487.t1 AT5G09400 43.580 810 422 12 5 789 59 858 0.0 632
MS.gene073487.t1 AT2G30070 43.586 647 309 2 143 789 2 592 0.0 556
MS.gene073487.t1 AT4G33530 45.219 617 324 6 184 789 14 627 0.0 546

Find 173 sgRNAs with CRISPR-Local

Find 279 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTTGGTTTCTCTTAAT+TGG 0.103523 2.4:-53076856 MS.gene073487:CDS
CAAAGTCTTGGTGTAGTTTA+TGG 0.148191 2.4:-53078045 MS.gene073487:CDS
TTGCTCCAATTGTTCTTCTT+TGG 0.191203 2.4:-53076870 MS.gene073487:CDS
ACAAGGAACCGCTCTTCTTC+TGG 0.211812 2.4:+53074789 None:intergenic
CGCAACTTCATTAAGCAATA+TGG 0.218556 2.4:-53074541 MS.gene073487:CDS
TACAGATGAGACTGGAAAAT+TGG 0.262617 2.4:+53076561 None:intergenic
GACAACACTGCTGATGATTT+TGG 0.271661 2.4:-53075213 MS.gene073487:CDS
AGATATCAATTGGATTCTTA+TGG 0.280779 2.4:-53076091 MS.gene073487:CDS
CTCTAACCATCTTTGCGTTT+TGG 0.284792 2.4:+53077369 None:intergenic
ATTCTTATTCTTGTCCTTGT+TGG 0.300340 2.4:-53077322 MS.gene073487:CDS
GGAATCTTGCTTAGCATAAC+AGG 0.312685 2.4:-53076715 MS.gene073487:intron
TTTCGGTGTGTGGCACGATA+TGG 0.313289 2.4:-53074735 MS.gene073487:CDS
TTCTGGACAGGCTGCATATC+TGG 0.318775 2.4:-53076491 MS.gene073487:CDS
TGGTGTAGTTTATGGAGATT+TGG 0.318941 2.4:-53078037 MS.gene073487:CDS
TACCATAAGCATTTCCAATC+TGG 0.319429 2.4:+53076028 None:intergenic
GTTATGTATGTTTGGCATTA+CGG 0.321291 2.4:-53075038 MS.gene073487:CDS
AAAGTAACATTCACATTTGT+TGG 0.321493 2.4:+53074474 None:intergenic
TTCTGATTGTTATGTATGTT+TGG 0.323587 2.4:-53075046 MS.gene073487:CDS
ATTATATATATGCATTTCTT+AGG 0.326736 2.4:-53074308 MS.gene073487:CDS
TGGGCTCCCCTTGCAATTGC+CGG 0.334267 2.4:-53075074 MS.gene073487:CDS
CAAAGGTAGTATGTGGGATT+TGG 0.334401 2.4:-53078331 MS.gene073487:CDS
TTTCTCTTAATTGGAGGTAT+AGG 0.338505 2.4:-53076847 MS.gene073487:CDS
GACACGGACTAGTCCTAAAC+TGG 0.341327 2.4:+53074947 None:intergenic
ATTTACAACTTGTGGAGATT+TGG 0.344580 2.4:-53076823 MS.gene073487:CDS
ATAAGAATCCAATTGATATC+TGG 0.358226 2.4:+53076093 None:intergenic
GTCTCAATGGCATGGATTCT+CGG 0.361185 2.4:-53074978 MS.gene073487:CDS
GTTGCTGTTGTAATATTAGT+TGG 0.362180 2.4:-53076940 MS.gene073487:CDS
CTATGCATTGCTGTTACTGC+TGG 0.364513 2.4:-53076066 MS.gene073487:CDS
ATTCTTACCCCAGCTATATC+TGG 0.365173 2.4:-53077274 MS.gene073487:intron
CTCGGGCTCGGTCCCAGTTT+AGG 0.365770 2.4:-53074960 MS.gene073487:CDS
ACCCAGATTGGAAATGCTTA+TGG 0.368806 2.4:-53076030 MS.gene073487:intron
GGTGTAGTTTATGGAGATTT+GGG 0.370924 2.4:-53078036 MS.gene073487:CDS
TGATATCTGGAATATATATC+TGG 0.375019 2.4:+53076106 None:intergenic
ATTTCTGAGCCTTCCTGCTT+CGG 0.381713 2.4:+53078281 None:intergenic
TATATATTCCAGATATCAAT+TGG 0.383953 2.4:-53076101 MS.gene073487:CDS
TCTCTCGACTGTTCGGTTTG+CGG 0.388246 2.4:+53074588 None:intergenic
CAAGGAACCGCTCTTCTTCT+GGG 0.399865 2.4:+53074790 None:intergenic
AGCATACCGCTATCAACTTC+GGG 0.405710 2.4:+53077098 None:intergenic
AGAAACCAAAGAAGAACAAT+TGG 0.406944 2.4:+53076865 None:intergenic
TGCGCTGTTGCTTCTACGAC+TGG 0.410868 2.4:-53078073 MS.gene073487:CDS
CAGCCGATGGATGCCGAAGC+AGG 0.412078 2.4:-53078294 MS.gene073487:CDS
CTTTGGTTTCTCTTAATTGG+AGG 0.412681 2.4:-53076853 MS.gene073487:CDS
CGGTTCCTTGTCAAGCGTAT+TGG 0.412864 2.4:-53074777 MS.gene073487:CDS
ACACAGTTGTGAGGGCTAGT+AGG 0.413822 2.4:-53074362 MS.gene073487:CDS
TGTGTTATAGAAAACGTATA+AGG 0.415069 2.4:+53078005 None:intergenic
GTGTAGACTAGGCCGATTCC+AGG 0.418903 2.4:+53074924 None:intergenic
TATGCACAACTTTGACTCTT+GGG 0.428719 2.4:+53076148 None:intergenic
ACCATAAGCATTTCCAATCT+GGG 0.433990 2.4:+53076029 None:intergenic
GTTGGCACCTGCATGGTGAT+TGG 0.434270 2.4:-53077304 MS.gene073487:CDS
GATAGTTGGCTTTCCCTTGG+TGG 0.434983 2.4:-53076736 MS.gene073487:CDS
CTGCTTCGGCATCCATCGGC+TGG 0.435634 2.4:+53078295 None:intergenic
AATCATCGTCTTTCTTATGT+AGG 0.436346 2.4:+53074703 None:intergenic
GTATGCACAACTTTGACTCT+TGG 0.445224 2.4:+53076147 None:intergenic
TATTAGTTTGGCACTGCCAT+TGG 0.448736 2.4:-53075184 MS.gene073487:CDS
TCAAAATCGATCAAGGTGGC+TGG 0.452735 2.4:-53075094 MS.gene073487:CDS
TCTCAATGGCATGGATTCTC+GGG 0.453151 2.4:-53074977 MS.gene073487:CDS
GCTGGTGTTGTTCACATACT+AGG 0.453676 2.4:-53074387 MS.gene073487:CDS
TTAAACAGCTGTAGAGAAGC+TGG 0.458023 2.4:-53074405 MS.gene073487:CDS
ACTTCTCATGGAAGGTAGTC+CGG 0.459359 2.4:+53077401 None:intergenic
CACAGTTGTGAGGGCTAGTA+GGG 0.463144 2.4:-53074361 MS.gene073487:CDS
TGCAGTTGTGTTAGTCATGC+TGG 0.464198 2.4:-53075237 MS.gene073487:CDS
TCATCAGTACGATGCTGGTT+CGG 0.464572 2.4:+53077442 None:intergenic
GATGATCCGGAGAAAGTGAT+TGG 0.464873 2.4:-53077967 MS.gene073487:CDS
CAAAACGCAAAGATGGTTAG+AGG 0.469945 2.4:-53077368 MS.gene073487:CDS
CATGAAATCTCTCGACTGTT+CGG 0.470466 2.4:+53074581 None:intergenic
ATGATAACAAAGGTAGTATG+TGG 0.475636 2.4:-53078338 MS.gene073487:CDS
ACATTTGTTGGTGATCTAGC+AGG 0.481805 2.4:+53074486 None:intergenic
TACAACTTGTGGAGATTTGG+TGG 0.485551 2.4:-53076820 MS.gene073487:CDS
ATTCTCGTTGTTGTGTTCAC+CGG 0.486407 2.4:-53075161 MS.gene073487:CDS
CTATTCAGCATACAACGTTA+TGG 0.487468 2.4:-53076916 MS.gene073487:CDS
TTGCTGCCAAAACGCAAAGA+TGG 0.487918 2.4:-53077375 MS.gene073487:CDS
TCCTCGTGGAGTTGATGATC+CGG 0.491615 2.4:-53077980 MS.gene073487:CDS
TTAGGACTAGTCCGTGTCCC+TGG 0.492338 2.4:-53074942 MS.gene073487:CDS
GAGCATACCGCTATCAACTT+CGG 0.495328 2.4:+53077097 None:intergenic
AGAGCTCCAATCACTTTCTC+CGG 0.496201 2.4:+53077961 None:intergenic
AAAGATAGTTGGCTTTCCCT+TGG 0.497018 2.4:-53076739 MS.gene073487:CDS
TGGCAGCAAATGACTTCTCA+TGG 0.498295 2.4:+53077389 None:intergenic
TGTCCTTGTTGGCACCTGCA+TGG 0.498812 2.4:-53077311 MS.gene073487:CDS
AGCGTTGCAATTACAAATAC+CGG 0.499486 2.4:+53076249 None:intergenic
CGAGCCGAACAAACATGAAA+AGG 0.499984 2.4:+53074658 None:intergenic
CACCGGCTTATCATTGATTG+TGG 0.505639 2.4:-53075144 MS.gene073487:CDS
CTAGTCCGTGTCCCTGGAAT+CGG 0.505874 2.4:-53074936 MS.gene073487:CDS
GTGTTATAGAAAACGTATAA+GGG 0.507909 2.4:+53078006 None:intergenic
ATGGCATGGATTCTCGGGCT+CGG 0.509081 2.4:-53074972 MS.gene073487:CDS
GACTGGAAAATTGGCTAAGT+CGG 0.509630 2.4:+53076570 None:intergenic
ATCTGCTGCTGGTGGCATCA+AGG 0.516150 2.4:-53077129 MS.gene073487:CDS
GTCCATGATGGAAGGTTGCT+CGG 0.516565 2.4:-53074634 MS.gene073487:CDS
AACAAGGACAAGAATAAGAA+TGG 0.518388 2.4:+53077324 None:intergenic
TCTTCTGGGACTGTGTAGAC+AGG 0.518745 2.4:+53074804 None:intergenic
GAAAACATACCAGATATAGC+TGG 0.518840 2.4:+53077265 None:intergenic
CTTCCTGCTTCGGCATCCAT+CGG 0.521217 2.4:+53078291 None:intergenic
TTTCACCTCTCTACGTGTAT+CGG 0.523357 2.4:-53076780 MS.gene073487:CDS
GTCCAGAATATGCTAAAAGA+AGG 0.523525 2.4:+53076506 None:intergenic
ATTAAGCAAGCCCTTGCTCA+TGG 0.527966 2.4:-53076177 MS.gene073487:CDS
CAAAATCGATCAAGGTGGCT+GGG 0.532334 2.4:-53075093 MS.gene073487:CDS
ATCCGAGCAACCTTCCATCA+TGG 0.532977 2.4:+53074632 None:intergenic
GAGACGGAGGCAAAGATAGT+TGG 0.533135 2.4:-53076750 MS.gene073487:CDS
ATTGCATAGCAAAGTCTCAA+TGG 0.534181 2.4:-53074991 MS.gene073487:CDS
TAATGAAATGAGAAAATATG+TGG 0.534737 2.4:+53074880 None:intergenic
GAAATACTCCGGCAATTGCA+AGG 0.535693 2.4:+53075066 None:intergenic
AAAACATACCAGATATAGCT+GGG 0.539177 2.4:+53077266 None:intergenic
GTACTCTTCAAAATCGATCA+AGG 0.539760 2.4:-53075101 MS.gene073487:CDS
ACTCTTCATCAGTACGATGC+TGG 0.545008 2.4:+53077437 None:intergenic
TGATCTTTATATTTGCATGT+CGG 0.548484 2.4:+53077467 None:intergenic
TGAAAAGGTTGCGGTGATTG+TGG 0.548496 2.4:+53076203 None:intergenic
GCAATTTGTACAGATGAGAC+TGG 0.550134 2.4:+53076553 None:intergenic
TTCCACAATCAATGATAAGC+CGG 0.550969 2.4:+53075142 None:intergenic
GGCTTGCTTAATGATTGAAA+AGG 0.551030 2.4:+53076188 None:intergenic
CTTAATGATTGAAAAGGTTG+CGG 0.555230 2.4:+53076194 None:intergenic
CGACTGGCATATCAAAGTCT+TGG 0.555965 2.4:-53078057 MS.gene073487:CDS
CATACATCAAAGAAATTCCT+CGG 0.556726 2.4:-53076129 MS.gene073487:CDS
GTATAGGTATTTACAACTTG+TGG 0.557069 2.4:-53076831 MS.gene073487:CDS
ATACACGTAGAGAGGTGAAA+AGG 0.557618 2.4:+53076783 None:intergenic
TGTTCGGCTCGAGTCCATGA+TGG 0.562025 2.4:-53074646 MS.gene073487:CDS
GTCTACACAGAGCTTGCAAG+TGG 0.564596 2.4:-53074909 MS.gene073487:CDS
CTGGAATATATATCTGGCCG+AGG 0.565446 2.4:+53076112 None:intergenic
TTGGGAAACATCCATGAGCA+AGG 0.566397 2.4:+53076166 None:intergenic
ATTTGCATGTCGGCAAAGCA+AGG 0.567672 2.4:+53077477 None:intergenic
CACGAGAGTCTGTTAAATGT+TGG 0.570415 2.4:-53074246 MS.gene073487:CDS
ACAACAACGGAATGGATCGC+TGG 0.570518 2.4:+53074849 None:intergenic
ACCGTCTCCAATCACCATGC+AGG 0.570556 2.4:+53077297 None:intergenic
CTGTGGAGCAGGTGGAACAC+TGG 0.572916 2.4:-53077509 MS.gene073487:intron
GATTTCATGTTGAATAACAA+TGG 0.574262 2.4:-53074567 MS.gene073487:CDS
AGGTTGCGGTGATTGTGGCT+TGG 0.574303 2.4:+53076208 None:intergenic
TTGCAAGCTCTGTGTAGACT+AGG 0.574990 2.4:+53074913 None:intergenic
AAATACTCCGGCAATTGCAA+GGG 0.576131 2.4:+53075067 None:intergenic
TGATAACAAAGGTAGTATGT+GGG 0.583248 2.4:-53078337 MS.gene073487:CDS
CAACGGAATGGATCGCTGGT+AGG 0.584685 2.4:+53074853 None:intergenic
AAACGAAAACAACAACGGAA+TGG 0.585282 2.4:+53074841 None:intergenic
GAATATGCTAAAAGAAGGCA+AGG 0.585635 2.4:+53076511 None:intergenic
ATGGTACAGATAGAGTTGGT+TGG 0.587725 2.4:-53076897 MS.gene073487:CDS
CATAACAATCAGAAATACTC+CGG 0.589596 2.4:+53075055 None:intergenic
CGATGGATGCCGAAGCAGGA+AGG 0.589706 2.4:-53078290 MS.gene073487:CDS
ACAAATTGCATTTACTCTCG+TGG 0.590570 2.4:-53076539 MS.gene073487:CDS
TTCTATAACACATTTCCTCG+TGG 0.594788 2.4:-53077994 MS.gene073487:CDS
AGCAAATGACTTCTCATGGA+AGG 0.597840 2.4:+53077393 None:intergenic
CGTTATGGTACAGATAGAGT+TGG 0.598043 2.4:-53076901 MS.gene073487:CDS
GATGAAGAAAATGATAACAA+AGG 0.598368 2.4:-53078348 MS.gene073487:CDS
AAGAGTTGACAACATATAGC+CGG 0.598791 2.4:-53077420 MS.gene073487:CDS
GTATGTTTGGCATTACGGAA+CGG 0.603324 2.4:-53075033 MS.gene073487:CDS
AGAGCAAATGACAATGGTCA+AGG 0.604083 2.4:-53077877 MS.gene073487:intron
CGGCTCGAGTCCATGATGGA+AGG 0.608365 2.4:-53074642 MS.gene073487:CDS
TCACCATGCAGGTGCCAACA+AGG 0.617937 2.4:+53077308 None:intergenic
ACAGAAACTTGACCAGCCGA+TGG 0.619273 2.4:-53078307 MS.gene073487:CDS
TGGGAAACATCCATGAGCAA+GGG 0.620410 2.4:+53076167 None:intergenic
GCTAAGCAAGATTCCACCAA+GGG 0.622974 2.4:+53076723 None:intergenic
ACCTGCATGGTGATTGGAGA+CGG 0.624117 2.4:-53077298 MS.gene073487:CDS
ACTAGGAAACACAGTTGTGA+GGG 0.624736 2.4:-53074370 MS.gene073487:CDS
TGTTACAGGAGTAGTAGTGC+TGG 0.627288 2.4:-53076962 MS.gene073487:intron
CTAGGCCGATTCCAGGGACA+CGG 0.628200 2.4:+53074931 None:intergenic
TGTAGACTAGGCCGATTCCA+GGG 0.629843 2.4:+53074925 None:intergenic
TCGGTCCAATACGCTTGACA+AGG 0.632001 2.4:+53074772 None:intergenic
TACTAGGAAACACAGTTGTG+AGG 0.636832 2.4:-53074371 MS.gene073487:CDS
GACACAAACGAAAACAACAA+CGG 0.645561 2.4:+53074836 None:intergenic
ATAGCAAAGTCTCAATGGCA+TGG 0.650531 2.4:-53074986 MS.gene073487:CDS
AATGAAATGAGAAAATATGT+GGG 0.652566 2.4:+53074881 None:intergenic
AATACTCCGGCAATTGCAAG+GGG 0.655579 2.4:+53075068 None:intergenic
ATAACAAACCTGGAATAGAA+CGG 0.658427 2.4:+53076434 None:intergenic
GTGTATCGGTATCTAAGAGA+CGG 0.666148 2.4:-53076766 MS.gene073487:CDS
AGATACCGATACACGTAGAG+AGG 0.668284 2.4:+53076775 None:intergenic
ATGAAAACGTATTTGAGCAG+CGG 0.669648 2.4:+53077907 None:intergenic
AACAAACCCGAAGTTGATAG+CGG 0.675102 2.4:-53077104 MS.gene073487:intron
ACACAACAACGAGAATCCAA+TGG 0.682411 2.4:+53075168 None:intergenic
GTATTAAGAGCAAATGACAA+TGG 0.692297 2.4:-53077883 MS.gene073487:CDS
TGCTAAGCAAGATTCCACCA+AGG 0.701088 2.4:+53076722 None:intergenic
ACACGGACTAGTCCTAAACT+GGG 0.702716 2.4:+53074948 None:intergenic
ATGAAATGAGAAAATATGTG+GGG 0.706454 2.4:+53074882 None:intergenic
ACATTTAACAGACTCTCGTG+AGG 0.708976 2.4:+53074249 None:intergenic
ACACAGTCCCAGAAGAAGAG+CGG 0.710327 2.4:-53074797 MS.gene073487:CDS
AAACATACCAGATATAGCTG+GGG 0.711575 2.4:+53077267 None:intergenic
TATCGGTATCTAAGAGACGG+AGG 0.713726 2.4:-53076763 MS.gene073487:CDS
CTCTTCAAAATCGATCAAGG+TGG 0.740278 2.4:-53075098 MS.gene073487:CDS
TCCGGATCATCAACTCCACG+AGG 0.743190 2.4:+53077979 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAACATGAAAAAAA+TGG + chr2.4:53076621-53076640 None:intergenic 10.0%
!! AAGAAATAAATTTATTAGAA+GGG - chr2.4:53076648-53076667 MS.gene073487:intron 10.0%
!! ATTATATATATGCATTTCTT+AGG - chr2.4:53078288-53078307 MS.gene073487:CDS 15.0%
!! GAAGAAATAAATTTATTAGA+AGG - chr2.4:53076647-53076666 MS.gene073487:intron 15.0%
!! TTGAAAAAATTTCTACAAAA+AGG + chr2.4:53075414-53075433 None:intergenic 15.0%
!! AAATTATTTAATAAGGTGAG+CGG - chr2.4:53075947-53075966 MS.gene073487:intron 20.0%
!! AATCATTACGACATTAAAAA+GGG - chr2.4:53076746-53076765 MS.gene073487:CDS 20.0%
!! AATGAAATGAGAAAATATGT+GGG + chr2.4:53077718-53077737 None:intergenic 20.0%
!! ACATTGAAAATTTGCAATAT+AGG - chr2.4:53076719-53076738 MS.gene073487:CDS 20.0%
!! AGAGAAAATGTATATATGTA+GGG + chr2.4:53077095-53077114 None:intergenic 20.0%
!! TAATCATTACGACATTAAAA+AGG - chr2.4:53076745-53076764 MS.gene073487:CDS 20.0%
!! TAATGAAATGAGAAAATATG+TGG + chr2.4:53077719-53077738 None:intergenic 20.0%
!! TAGAGAAAATGTATATATGT+AGG + chr2.4:53077096-53077115 None:intergenic 20.0%
!! TATATATTCCAGATATCAAT+TGG - chr2.4:53076495-53076514 MS.gene073487:CDS 20.0%
!!! AAAAAAGTGTAACGTAATTA+GGG - chr2.4:53076971-53076990 MS.gene073487:intron 20.0%
!!! TTAATGTATGTTAAAAGTGT+AGG + chr2.4:53075524-53075543 None:intergenic 20.0%
!!! TTTGGTTAATTTGGATATTA+TGG - chr2.4:53074762-53074781 MS.gene073487:CDS 20.0%
! AAAGGAAAGTCAAAAATGAA+AGG + chr2.4:53075396-53075415 None:intergenic 25.0%
! AAAGTAACATTCACATTTGT+TGG + chr2.4:53078125-53078144 None:intergenic 25.0%
! AATGATCTAGTATATCTTCT+TGG + chr2.4:53077267-53077286 None:intergenic 25.0%
! AATGCAGCTGAAAATTTAAA+GGG - chr2.4:53077016-53077035 MS.gene073487:intron 25.0%
! ACCTATTCAATAAATGCTAA+AGG + chr2.4:53074851-53074870 None:intergenic 25.0%
! ACCTGTACTAAATAATATGA+TGG + chr2.4:53076946-53076965 None:intergenic 25.0%
! AGATATCAATTGGATTCTTA+TGG - chr2.4:53076505-53076524 MS.gene073487:CDS 25.0%
! AGTTCTTAAACTTTGTTAAC+TGG + chr2.4:53077292-53077311 None:intergenic 25.0%
! ATAAGAATCCAATTGATATC+TGG + chr2.4:53076506-53076525 None:intergenic 25.0%
! ATGAAATGAGAAAATATGTG+GGG + chr2.4:53077717-53077736 None:intergenic 25.0%
! CAATGTAAAGAAATTAGGAA+AGG + chr2.4:53076772-53076791 None:intergenic 25.0%
! CCTAATTTCTTTACATTGAA+GGG - chr2.4:53076774-53076793 MS.gene073487:CDS 25.0%
! CTATAATCTCATATACATAG+TGG + chr2.4:53075560-53075579 None:intergenic 25.0%
! GAATTCAGAAAAAGCAAAAA+TGG + chr2.4:53074378-53074397 None:intergenic 25.0%
! GATGAAGAAAATGATAACAA+AGG - chr2.4:53074248-53074267 MS.gene073487:CDS 25.0%
! GATTTCATGTTGAATAACAA+TGG - chr2.4:53078029-53078048 MS.gene073487:CDS 25.0%
! GTGTTATAGAAAACGTATAA+GGG + chr2.4:53074593-53074612 None:intergenic 25.0%
! TAATGCAGCTGAAAATTTAA+AGG - chr2.4:53077015-53077034 MS.gene073487:intron 25.0%
! TAGGATCCTATATTTAGAAA+CGG - chr2.4:53077063-53077082 MS.gene073487:intron 25.0%
! TCCATCATATTATTTAGTAC+AGG - chr2.4:53076942-53076961 MS.gene073487:CDS 25.0%
! TCCTAATTTCTTTACATTGA+AGG - chr2.4:53076773-53076792 MS.gene073487:CDS 25.0%
! TGATCTTTATATTTGCATGT+CGG + chr2.4:53075132-53075151 None:intergenic 25.0%
! TGTGTTATAGAAAACGTATA+AGG + chr2.4:53074594-53074613 None:intergenic 25.0%
! TTCTGATTGTTATGTATGTT+TGG - chr2.4:53077550-53077569 MS.gene073487:intron 25.0%
! TTTAAAAATCAAACCCAGAT+TGG - chr2.4:53076554-53076573 MS.gene073487:CDS 25.0%
!! AAAAAGTGTAACGTAATTAG+GGG - chr2.4:53076972-53076991 MS.gene073487:intron 25.0%
!! CAATGATTTTTATCTGTTAG+AGG - chr2.4:53076290-53076309 MS.gene073487:intron 25.0%
!! GAAAAAAGTGTAACGTAATT+AGG - chr2.4:53076970-53076989 MS.gene073487:intron 25.0%
!! GTTAATTTGGATATTATGGT+TGG - chr2.4:53074766-53074785 MS.gene073487:CDS 25.0%
!! TGATATCTGGAATATATATC+TGG + chr2.4:53076493-53076512 None:intergenic 25.0%
!! TTTTCTTCATCAATCTCTAA+CGG + chr2.4:53074239-53074258 None:intergenic 25.0%
!! TTTTGGTCATGATATTAGTT+TGG - chr2.4:53077400-53077419 MS.gene073487:CDS 25.0%
!!! ATAGTTTCGAGTTTTTTCAT+TGG - chr2.4:53075576-53075595 MS.gene073487:intron 25.0%
!!! TTGTTTTTCAGAAGCTATTT+TGG - chr2.4:53074895-53074914 MS.gene073487:CDS 25.0%
AAAAATCATTGCATGAAGAC+AGG + chr2.4:53076281-53076300 None:intergenic 30.0%
AAAACATACCAGATATAGCT+GGG + chr2.4:53075333-53075352 None:intergenic 30.0%
AAAATCATTGCATGAAGACA+GGG + chr2.4:53076280-53076299 None:intergenic 30.0%
AACAAGGACAAGAATAAGAA+TGG + chr2.4:53075275-53075294 None:intergenic 30.0%
AATCATCGTCTTTCTTATGT+AGG + chr2.4:53077896-53077915 None:intergenic 30.0%
AGAAACCAAAGAAGAACAAT+TGG + chr2.4:53075734-53075753 None:intergenic 30.0%
AGTAGCTGAACATAACTAAA+CGG - chr2.4:53077231-53077250 MS.gene073487:intron 30.0%
ATAACAAACCTGGAATAGAA+CGG + chr2.4:53076165-53076184 None:intergenic 30.0%
ATCCAAATTAACCAAACAGT+TGG + chr2.4:53074758-53074777 None:intergenic 30.0%
ATCCTATATTTAGAAACGGA+GGG - chr2.4:53077067-53077086 MS.gene073487:intron 30.0%
ATGATAACAAAGGTAGTATG+TGG - chr2.4:53074258-53074277 MS.gene073487:CDS 30.0%
ATGCAGCTGAAAATTTAAAG+GGG - chr2.4:53077017-53077036 MS.gene073487:intron 30.0%
ATTCTTATTCTTGTCCTTGT+TGG - chr2.4:53075274-53075293 MS.gene073487:intron 30.0%
ATTTACAACTTGTGGAGATT+TGG - chr2.4:53075773-53075792 MS.gene073487:intron 30.0%
CATAACAATCAGAAATACTC+CGG + chr2.4:53077544-53077563 None:intergenic 30.0%
CATACATCAAAGAAATTCCT+CGG - chr2.4:53076467-53076486 MS.gene073487:CDS 30.0%
CCCTTCAATGTAAAGAAATT+AGG + chr2.4:53076777-53076796 None:intergenic 30.0%
CGAAAAATGTGGTAATTCTT+CGG + chr2.4:53077846-53077865 None:intergenic 30.0%
CGTATGTTGTCTATAAAAGT+GGG - chr2.4:53077173-53077192 MS.gene073487:intron 30.0%
CTTAATGATTGAAAAGGTTG+CGG + chr2.4:53076405-53076424 None:intergenic 30.0%
GAATTTATCCTTGTAATGAC+AGG - chr2.4:53077333-53077352 MS.gene073487:CDS 30.0%
GCCTTTAGCATTTATTGAAT+AGG - chr2.4:53074847-53074866 MS.gene073487:CDS 30.0%
GGAGGAACTAACAAATAAAT+AGG + chr2.4:53075375-53075394 None:intergenic 30.0%
GTATAGGTATTTACAACTTG+TGG - chr2.4:53075765-53075784 MS.gene073487:intron 30.0%
GTATTAAGAGCAAATGACAA+TGG - chr2.4:53074713-53074732 MS.gene073487:CDS 30.0%
GTTATGTATGTTTGGCATTA+CGG - chr2.4:53077558-53077577 MS.gene073487:intron 30.0%
GTTCTGCAGAAAAAATATAC+TGG - chr2.4:53076324-53076343 MS.gene073487:intron 30.0%
TCGTATGTTGTCTATAAAAG+TGG - chr2.4:53077172-53077191 MS.gene073487:intron 30.0%
TGATAACAAAGGTAGTATGT+GGG - chr2.4:53074259-53074278 MS.gene073487:CDS 30.0%
TTCCAACTGTTTGGTTAATT+TGG - chr2.4:53074753-53074772 MS.gene073487:CDS 30.0%
! ACAACCTTTTCATGTTTGTT+CGG - chr2.4:53077934-53077953 MS.gene073487:CDS 30.0%
! CGAAGAATTACCACATTTTT+CGG - chr2.4:53077844-53077863 MS.gene073487:intron 30.0%
! CTTCTTTGGTTTCTCTTAAT+TGG - chr2.4:53075740-53075759 MS.gene073487:intron 30.0%
! GTTGCTGTTGTAATATTAGT+TGG - chr2.4:53075656-53075675 MS.gene073487:intron 30.0%
! TAACGTAATTAGGGGTATAT+TGG - chr2.4:53076980-53076999 MS.gene073487:intron 30.0%
! TGTATTGTTTTCCAACTGTT+TGG - chr2.4:53074744-53074763 MS.gene073487:CDS 30.0%
! TGTGGAATGCACATATTTTT+CGG - chr2.4:53077470-53077489 MS.gene073487:CDS 30.0%
! TTTCTCTTAATTGGAGGTAT+AGG - chr2.4:53075749-53075768 MS.gene073487:intron 30.0%
AAACATACCAGATATAGCTG+GGG + chr2.4:53075332-53075351 None:intergenic 35.0%
AAACGAAAACAACAACGGAA+TGG + chr2.4:53077758-53077777 None:intergenic 35.0%
AAACTTATCATCTCTCCTCA+AGG + chr2.4:53076917-53076936 None:intergenic 35.0%
AAGAGTTGACAACATATAGC+CGG - chr2.4:53075176-53075195 MS.gene073487:CDS 35.0%
ACAAAGTAAGCGTTCATTTC+TGG + chr2.4:53077142-53077161 None:intergenic 35.0%
ACAAATTGCATTTACTCTCG+TGG - chr2.4:53076057-53076076 MS.gene073487:CDS 35.0%
ACCATAAGCATTTCCAATCT+GGG + chr2.4:53076570-53076589 None:intergenic 35.0%
AGAGAGAAAATGCAACTTGT+AGG - chr2.4:53076671-53076690 MS.gene073487:intron 35.0%
AGCGTTGCAATTACAAATAC+CGG + chr2.4:53076350-53076369 None:intergenic 35.0%
ATGAAAACGTATTTGAGCAG+CGG + chr2.4:53074692-53074711 None:intergenic 35.0%
ATTGCATAGCAAAGTCTCAA+TGG - chr2.4:53077605-53077624 MS.gene073487:intron 35.0%
CATTACAAGGATAAATTCGC+TGG + chr2.4:53077331-53077350 None:intergenic 35.0%
CGCAACTTCATTAAGCAATA+TGG - chr2.4:53078055-53078074 MS.gene073487:CDS 35.0%
CTATTCAGCATACAACGTTA+TGG - chr2.4:53075680-53075699 MS.gene073487:intron 35.0%
GAAAACATACCAGATATAGC+TGG + chr2.4:53075334-53075353 None:intergenic 35.0%
GAATATGCTAAAAGAAGGCA+AGG + chr2.4:53076088-53076107 None:intergenic 35.0%
GACACAAACGAAAACAACAA+CGG + chr2.4:53077763-53077782 None:intergenic 35.0%
GATCCTATATTTAGAAACGG+AGG - chr2.4:53077066-53077085 MS.gene073487:intron 35.0%
GGAAAGTCAAAAATGAAAGG+AGG + chr2.4:53075393-53075412 None:intergenic 35.0%
GGTGTAGTTTATGGAGATTT+GGG - chr2.4:53074560-53074579 MS.gene073487:CDS 35.0%
GTACTCTTCAAAATCGATCA+AGG - chr2.4:53077495-53077514 MS.gene073487:CDS 35.0%
GTCCAGAATATGCTAAAAGA+AGG + chr2.4:53076093-53076112 None:intergenic 35.0%
GTTGACGATAAACTGCATAT+TGG + chr2.4:53077120-53077139 None:intergenic 35.0%
TACAGATGAGACTGGAAAAT+TGG + chr2.4:53076038-53076057 None:intergenic 35.0%
TACCATAAGCATTTCCAATC+TGG + chr2.4:53076571-53076590 None:intergenic 35.0%
TATGCACAACTTTGACTCTT+GGG + chr2.4:53076451-53076470 None:intergenic 35.0%
TGCAGAAAAAATATACTGGC+CGG - chr2.4:53076328-53076347 MS.gene073487:intron 35.0%
TGCAGCTGAAAATTTAAAGG+GGG - chr2.4:53077018-53077037 MS.gene073487:intron 35.0%
TGGATATTATGGTTGGACTA+AGG - chr2.4:53074773-53074792 MS.gene073487:CDS 35.0%
TGGTGTAGTTTATGGAGATT+TGG - chr2.4:53074559-53074578 MS.gene073487:CDS 35.0%
TGTGACAGATATAACAAACC+TGG + chr2.4:53076175-53076194 None:intergenic 35.0%
TTCCACAATCAATGATAAGC+CGG + chr2.4:53077457-53077476 None:intergenic 35.0%
TTCTATAACACATTTCCTCG+TGG - chr2.4:53074602-53074621 MS.gene073487:CDS 35.0%
TTGCTCCAATTGTTCTTCTT+TGG - chr2.4:53075726-53075745 MS.gene073487:intron 35.0%
TTTAAAGGGGGCCTATAAAA+CGG - chr2.4:53077030-53077049 MS.gene073487:intron 35.0%
TTTAACCAAGCTTACATCTG+TGG - chr2.4:53075070-53075089 MS.gene073487:CDS 35.0%
! AACTTGTGACGATTGATTTC+AGG - chr2.4:53075986-53076005 MS.gene073487:intron 35.0%
! ACTTGTGACGATTGATTTCA+GGG - chr2.4:53075987-53076006 MS.gene073487:intron 35.0%
! CAAAGTCTTGGTGTAGTTTA+TGG - chr2.4:53074551-53074570 MS.gene073487:CDS 35.0%
! CTTTGGTTTCTCTTAATTGG+AGG - chr2.4:53075743-53075762 MS.gene073487:intron 35.0%
! GGCTTGCTTAATGATTGAAA+AGG + chr2.4:53076411-53076430 None:intergenic 35.0%
! TCTTTTAGCATATTCTGGAC+AGG - chr2.4:53076093-53076112 MS.gene073487:CDS 35.0%
! TGCCTTCTTTTAGCATATTC+TGG - chr2.4:53076088-53076107 MS.gene073487:CDS 35.0%
!! TGCACTTTTGTTTCTTCATC+AGG - chr2.4:53076236-53076255 MS.gene073487:CDS 35.0%
!!! GTGAAAAGGCTTTTAAGACA+CGG + chr2.4:53075802-53075821 None:intergenic 35.0%
AAATACTCCGGCAATTGCAA+GGG + chr2.4:53077532-53077551 None:intergenic 40.0%
ACACAACAACGAGAATCCAA+TGG + chr2.4:53077431-53077450 None:intergenic 40.0%
ACATTTAACAGACTCTCGTG+AGG + chr2.4:53078350-53078369 None:intergenic 40.0%
ACCCAGATTGGAAATGCTTA+TGG - chr2.4:53076566-53076585 MS.gene073487:CDS 40.0%
AGAGCAAATGACAATGGTCA+AGG - chr2.4:53074719-53074738 MS.gene073487:CDS 40.0%
AGCAAATGACTTCTCATGGA+AGG + chr2.4:53075206-53075225 None:intergenic 40.0%
ATAAAACGGGACTGGGAAAT+AGG - chr2.4:53077044-53077063 MS.gene073487:intron 40.0%
ATACACGTAGAGAGGTGAAA+AGG + chr2.4:53075816-53075835 None:intergenic 40.0%
ATAGCAAAGTCTCAATGGCA+TGG - chr2.4:53077610-53077629 MS.gene073487:intron 40.0%
ATTCTCGTTGTTGTGTTCAC+CGG - chr2.4:53077435-53077454 MS.gene073487:CDS 40.0%
ATTCTTACCCCAGCTATATC+TGG - chr2.4:53075322-53075341 MS.gene073487:intron 40.0%
CAAAACGCAAAGATGGTTAG+AGG - chr2.4:53075228-53075247 MS.gene073487:CDS 40.0%
CAAAGGTAGTATGTGGGATT+TGG - chr2.4:53074265-53074284 MS.gene073487:CDS 40.0%
CACGAGAGTCTGTTAAATGT+TGG - chr2.4:53078350-53078369 MS.gene073487:CDS 40.0%
CATGAAATCTCTCGACTGTT+CGG + chr2.4:53078018-53078037 None:intergenic 40.0%
CGTTATGGTACAGATAGAGT+TGG - chr2.4:53075695-53075714 MS.gene073487:intron 40.0%
CTAACTTCGTCCCTAGATAT+AGG - chr2.4:53076808-53076827 MS.gene073487:CDS 40.0%
CTCCCTCCGTTTCTAAATAT+AGG + chr2.4:53077072-53077091 None:intergenic 40.0%
CTCTTCAAAATCGATCAAGG+TGG - chr2.4:53077498-53077517 MS.gene073487:CDS 40.0%
CTTCATCAGGAGCAAAATGT+GGG - chr2.4:53076249-53076268 MS.gene073487:CDS 40.0%
GACTGGAAAATTGGCTAAGT+CGG + chr2.4:53076029-53076048 None:intergenic 40.0%
GAGAAAATGCAACTTGTAGG+AGG - chr2.4:53076674-53076693 MS.gene073487:intron 40.0%
GCAATTTGTACAGATGAGAC+TGG + chr2.4:53076046-53076065 None:intergenic 40.0%
GGAATCTTGCTTAGCATAAC+AGG - chr2.4:53075881-53075900 MS.gene073487:intron 40.0%
GTATGCACAACTTTGACTCT+TGG + chr2.4:53076452-53076471 None:intergenic 40.0%
GTATGTTTGGCATTACGGAA+CGG - chr2.4:53077563-53077582 MS.gene073487:intron 40.0%
GTCAACGAGTCCTATATCTA+GGG + chr2.4:53076821-53076840 None:intergenic 40.0%
GTTTCGCAAGTTTATCCTTG+AGG - chr2.4:53076899-53076918 MS.gene073487:CDS 40.0%
TACAACTTGTGGAGATTTGG+TGG - chr2.4:53075776-53075795 MS.gene073487:intron 40.0%
TCTTCATCAGGAGCAAAATG+TGG - chr2.4:53076248-53076267 MS.gene073487:CDS 40.0%
TTAAACAGCTGTAGAGAAGC+TGG - chr2.4:53078191-53078210 MS.gene073487:intron 40.0%
TTAAAGGGGGCCTATAAAAC+GGG - chr2.4:53077031-53077050 MS.gene073487:intron 40.0%
TTGATGCAAGAGTAAGAGAG+TGG - chr2.4:53074812-53074831 MS.gene073487:CDS 40.0%
TTTCACCTCTCTACGTGTAT+CGG - chr2.4:53075816-53075835 MS.gene073487:intron 40.0%
! ATGGTACAGATAGAGTTGGT+TGG - chr2.4:53075699-53075718 MS.gene073487:intron 40.0%
! ATTTCCCAGTCCCGTTTTAT+AGG + chr2.4:53077044-53077063 None:intergenic 40.0%
! CTCTAACCATCTTTGCGTTT+TGG + chr2.4:53075230-53075249 None:intergenic 40.0%
! GACAACACTGCTGATGATTT+TGG - chr2.4:53077383-53077402 MS.gene073487:CDS 40.0%
! GTAAGTTTTTCGCTTGCAGA+AGG - chr2.4:53076588-53076607 MS.gene073487:CDS 40.0%
! GTGTATCGGTATCTAAGAGA+CGG - chr2.4:53075830-53075849 MS.gene073487:intron 40.0%
! GTGTTTTACCGTTCTATTCC+AGG - chr2.4:53076154-53076173 MS.gene073487:CDS 40.0%
! TAACACCATGGCGGAAAAAA+GGG + chr2.4:53075619-53075638 None:intergenic 40.0%
! TATTAGTTTGGCACTGCCAT+TGG - chr2.4:53077412-53077431 MS.gene073487:CDS 40.0%
! TTTTTCCCTTTTTTCCGCCA+TGG - chr2.4:53075611-53075630 MS.gene073487:intron 40.0%
!! AAAGATAGTTGGCTTTCCCT+TGG - chr2.4:53075857-53075876 MS.gene073487:intron 40.0%
!! AACAAACCCGAAGTTGATAG+CGG - chr2.4:53075492-53075511 MS.gene073487:intron 40.0%
!! ACATTTGTTGGTGATCTAGC+AGG + chr2.4:53078113-53078132 None:intergenic 40.0%
!! TTACCACATTTTTCGGTGTG+TGG - chr2.4:53077851-53077870 MS.gene073487:intron 40.0%
!!! GATGTAGTTTTATCTGCTGC+TGG - chr2.4:53075456-53075475 MS.gene073487:intron 40.0%
AATACTCCGGCAATTGCAAG+GGG + chr2.4:53077531-53077550 None:intergenic 45.0%
ACTCTTCATCAGTACGATGC+TGG + chr2.4:53075162-53075181 None:intergenic 45.0%
ACTTCTCATGGAAGGTAGTC+CGG + chr2.4:53075198-53075217 None:intergenic 45.0%
AGAGCTCCAATCACTTTCTC+CGG + chr2.4:53074638-53074657 None:intergenic 45.0%
AGATACCGATACACGTAGAG+AGG + chr2.4:53075824-53075843 None:intergenic 45.0%
AGCATACCGCTATCAACTTC+GGG + chr2.4:53075501-53075520 None:intergenic 45.0%
AGGACTCGTTGACCAAATCA+CGG - chr2.4:53076828-53076847 MS.gene073487:CDS 45.0%
ATTAAGCAAGCCCTTGCTCA+TGG - chr2.4:53076419-53076438 MS.gene073487:intron 45.0%
ATTTCTGAGCCTTCCTGCTT+CGG + chr2.4:53074318-53074337 None:intergenic 45.0%
ATTTGCATGTCGGCAAAGCA+AGG + chr2.4:53075122-53075141 None:intergenic 45.0%
CAAAATCGATCAAGGTGGCT+GGG - chr2.4:53077503-53077522 MS.gene073487:intron 45.0%
CGACTGGCATATCAAAGTCT+TGG - chr2.4:53074539-53074558 MS.gene073487:CDS 45.0%
CGAGCCGAACAAACATGAAA+AGG + chr2.4:53077941-53077960 None:intergenic 45.0%
CTACTACTCCTGTAACACCA+TGG + chr2.4:53075631-53075650 None:intergenic 45.0%
CTGGAATATATATCTGGCCG+AGG + chr2.4:53076487-53076506 None:intergenic 45.0%
CTTTCTTAGCTCCCGTGATT+TGG + chr2.4:53076843-53076862 None:intergenic 45.0%
GAAATACTCCGGCAATTGCA+AGG + chr2.4:53077533-53077552 None:intergenic 45.0%
GAGCATACCGCTATCAACTT+CGG + chr2.4:53075502-53075521 None:intergenic 45.0%
GATGATCCGGAGAAAGTGAT+TGG - chr2.4:53074629-53074648 MS.gene073487:CDS 45.0%
GCTAAGCAAGATTCCACCAA+GGG + chr2.4:53075876-53075895 None:intergenic 45.0%
GGTCAACGAGTCCTATATCT+AGG + chr2.4:53076822-53076841 None:intergenic 45.0%
GTCTCAATGGCATGGATTCT+CGG - chr2.4:53077618-53077637 MS.gene073487:intron 45.0%
TATCGGTATCTAAGAGACGG+AGG - chr2.4:53075833-53075852 MS.gene073487:intron 45.0%
TCAAAATCGATCAAGGTGGC+TGG - chr2.4:53077502-53077521 MS.gene073487:intron 45.0%
TCTCAATGGCATGGATTCTC+GGG - chr2.4:53077619-53077638 MS.gene073487:intron 45.0%
TGAAAAGGTTGCGGTGATTG+TGG + chr2.4:53076396-53076415 None:intergenic 45.0%
TGCAGTTGTGTTAGTCATGC+TGG - chr2.4:53077359-53077378 MS.gene073487:CDS 45.0%
TGCTAAGCAAGATTCCACCA+AGG + chr2.4:53075877-53075896 None:intergenic 45.0%
TGGCAGCAAATGACTTCTCA+TGG + chr2.4:53075210-53075229 None:intergenic 45.0%
TGGGAAACATCCATGAGCAA+GGG + chr2.4:53076432-53076451 None:intergenic 45.0%
TGTTACAGGAGTAGTAGTGC+TGG - chr2.4:53075634-53075653 MS.gene073487:intron 45.0%
TTGCAAGCTCTGTGTAGACT+AGG + chr2.4:53077686-53077705 None:intergenic 45.0%
TTGCTGCCAAAACGCAAAGA+TGG - chr2.4:53075221-53075240 MS.gene073487:CDS 45.0%
TTGGGAAACATCCATGAGCA+AGG + chr2.4:53076433-53076452 None:intergenic 45.0%
! CTATGCATTGCTGTTACTGC+TGG - chr2.4:53076530-53076549 MS.gene073487:CDS 45.0%
! GTAACACCATGGCGGAAAAA+AGG + chr2.4:53075620-53075639 None:intergenic 45.0%
! TCATCAGTACGATGCTGGTT+CGG + chr2.4:53075157-53075176 None:intergenic 45.0%
!! ACACGGACTAGTCCTAAACT+GGG + chr2.4:53077651-53077670 None:intergenic 45.0%
!! CACCGGCTTATCATTGATTG+TGG - chr2.4:53077452-53077471 MS.gene073487:CDS 45.0%
!!! GTAGTTTTATCTGCTGCTGG+TGG - chr2.4:53075459-53075478 MS.gene073487:intron 45.0%
!!! TTTTTCCGCCATGGTGTTAC+AGG - chr2.4:53075620-53075639 MS.gene073487:intron 45.0%
!!! TGTTTTAAAATTATTTAATA+AGG - chr2.4:53075940-53075959 MS.gene073487:intron 5.0%
ACAACAACGGAATGGATCGC+TGG + chr2.4:53077750-53077769 None:intergenic 50.0%
ACAAGGAACCGCTCTTCTTC+TGG + chr2.4:53077810-53077829 None:intergenic 50.0%
ACACAGTCCCAGAAGAAGAG+CGG - chr2.4:53077799-53077818 MS.gene073487:intron 50.0%
ACAGAAACTTGACCAGCCGA+TGG - chr2.4:53074289-53074308 MS.gene073487:CDS 50.0%
ACCTGCATGGTGATTGGAGA+CGG - chr2.4:53075298-53075317 MS.gene073487:intron 50.0%
ATCCGAGCAACCTTCCATCA+TGG + chr2.4:53077967-53077986 None:intergenic 50.0%
CAAGCTTACATCTGTGGAGC+AGG - chr2.4:53075076-53075095 MS.gene073487:CDS 50.0%
CAAGGAACCGCTCTTCTTCT+GGG + chr2.4:53077809-53077828 None:intergenic 50.0%
CGGTTCCTTGTCAAGCGTAT+TGG - chr2.4:53077819-53077838 MS.gene073487:intron 50.0%
CTACTCCTGTAACACCATGG+CGG + chr2.4:53075628-53075647 None:intergenic 50.0%
CTGCAGTGCCTGTCATTACA+AGG + chr2.4:53077344-53077363 None:intergenic 50.0%
CTGCTCCACAGATGTAAGCT+TGG + chr2.4:53075078-53075097 None:intergenic 50.0%
GAGACGGAGGCAAAGATAGT+TGG - chr2.4:53075846-53075865 MS.gene073487:intron 50.0%
GGACTCGTTGACCAAATCAC+GGG - chr2.4:53076829-53076848 MS.gene073487:CDS 50.0%
GTCTACACAGAGCTTGCAAG+TGG - chr2.4:53077687-53077706 MS.gene073487:intron 50.0%
GTGCCACACACCGAAAAATG+TGG + chr2.4:53077857-53077876 None:intergenic 50.0%
TCGGTCCAATACGCTTGACA+AGG + chr2.4:53077827-53077846 None:intergenic 50.0%
TTCTGGACAGGCTGCATATC+TGG - chr2.4:53076105-53076124 MS.gene073487:CDS 50.0%
! ACACAGTTGTGAGGGCTAGT+AGG - chr2.4:53078234-53078253 MS.gene073487:intron 50.0%
! CACAGTTGTGAGGGCTAGTA+GGG - chr2.4:53078235-53078254 MS.gene073487:intron 50.0%
! GTCCATGATGGAAGGTTGCT+CGG - chr2.4:53077962-53077981 MS.gene073487:CDS 50.0%
! TCTCTCGACTGTTCGGTTTG+CGG + chr2.4:53078011-53078030 None:intergenic 50.0%
! TGTAGACTAGGCCGATTCCA+GGG + chr2.4:53077674-53077693 None:intergenic 50.0%
! TTTCGGTGTGTGGCACGATA+TGG - chr2.4:53077861-53077880 MS.gene073487:intron 50.0%
!! GACACGGACTAGTCCTAAAC+TGG + chr2.4:53077652-53077671 None:intergenic 50.0%
!! GATAGTTGGCTTTCCCTTGG+TGG - chr2.4:53075860-53075879 MS.gene073487:intron 50.0%
!! TCCTCGTGGAGTTGATGATC+CGG - chr2.4:53074616-53074635 MS.gene073487:CDS 50.0%
!! TCTTCTGGGACTGTGTAGAC+AGG + chr2.4:53077795-53077814 None:intergenic 50.0%
ACCGTCTCCAATCACCATGC+AGG + chr2.4:53075302-53075321 None:intergenic 55.0%
AGGTTGCGGTGATTGTGGCT+TGG + chr2.4:53076391-53076410 None:intergenic 55.0%
ATCTGCTGCTGGTGGCATCA+AGG - chr2.4:53075467-53075486 MS.gene073487:intron 55.0%
ATGGCATGGATTCTCGGGCT+CGG - chr2.4:53077624-53077643 MS.gene073487:intron 55.0%
CAACGGAATGGATCGCTGGT+AGG + chr2.4:53077746-53077765 None:intergenic 55.0%
CTAGTCCGTGTCCCTGGAAT+CGG - chr2.4:53077660-53077679 MS.gene073487:intron 55.0%
CTTCCTGCTTCGGCATCCAT+CGG + chr2.4:53074308-53074327 None:intergenic 55.0%
GCTTACATCTGTGGAGCAGG+TGG - chr2.4:53075079-53075098 MS.gene073487:CDS 55.0%
GGGGCCTATAAAACGGGACT+GGG - chr2.4:53077037-53077056 MS.gene073487:intron 55.0%
GTTGGCACCTGCATGGTGAT+TGG - chr2.4:53075292-53075311 MS.gene073487:intron 55.0%
TCACCATGCAGGTGCCAACA+AGG + chr2.4:53075291-53075310 None:intergenic 55.0%
TCCGGATCATCAACTCCACG+AGG + chr2.4:53074620-53074639 None:intergenic 55.0%
TGTCCTTGTTGGCACCTGCA+TGG - chr2.4:53075285-53075304 MS.gene073487:intron 55.0%
! GTGTAGACTAGGCCGATTCC+AGG + chr2.4:53077675-53077694 None:intergenic 55.0%
! TGCGCTGTTGCTTCTACGAC+TGG - chr2.4:53074523-53074542 MS.gene073487:CDS 55.0%
! TGTTCGGCTCGAGTCCATGA+TGG - chr2.4:53077950-53077969 MS.gene073487:CDS 55.0%
!! TTAGGACTAGTCCGTGTCCC+TGG - chr2.4:53077654-53077673 MS.gene073487:intron 55.0%
CGATGGATGCCGAAGCAGGA+AGG - chr2.4:53074306-53074325 MS.gene073487:CDS 60.0%
CTGTGGAGCAGGTGGAACAC+TGG - chr2.4:53075087-53075106 MS.gene073487:CDS 60.0%
GGGGGCCTATAAAACGGGAC+TGG - chr2.4:53077036-53077055 MS.gene073487:intron 60.0%
TGGGCTCCCCTTGCAATTGC+CGG - chr2.4:53077522-53077541 MS.gene073487:intron 60.0%
! CGGCTCGAGTCCATGATGGA+AGG - chr2.4:53077954-53077973 MS.gene073487:CDS 60.0%
! CTAGGCCGATTCCAGGGACA+CGG + chr2.4:53077668-53077687 None:intergenic 60.0%
CAGCCGATGGATGCCGAAGC+AGG - chr2.4:53074302-53074321 MS.gene073487:CDS 65.0%
CTGCTTCGGCATCCATCGGC+TGG + chr2.4:53074304-53074323 None:intergenic 65.0%
! CTCGGGCTCGGTCCCAGTTT+AGG - chr2.4:53077636-53077655 MS.gene073487:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 53074227 53078391 53074227 ID=MS.gene073487
chr2.4 mRNA 53074227 53078391 53074227 ID=MS.gene073487.t1;Parent=MS.gene073487
chr2.4 exon 53078266 53078391 53078266 ID=MS.gene073487.t1.exon1;Parent=MS.gene073487.t1
chr2.4 CDS 53078266 53078391 53078266 ID=cds.MS.gene073487.t1;Parent=MS.gene073487.t1
chr2.4 exon 53077878 53078103 53077878 ID=MS.gene073487.t1.exon2;Parent=MS.gene073487.t1
chr2.4 CDS 53077878 53078103 53077878 ID=cds.MS.gene073487.t1;Parent=MS.gene073487.t1
chr2.4 exon 53077275 53077520 53077275 ID=MS.gene073487.t1.exon3;Parent=MS.gene073487.t1
chr2.4 CDS 53077275 53077520 53077275 ID=cds.MS.gene073487.t1;Parent=MS.gene073487.t1
chr2.4 exon 53077105 53077155 53077105 ID=MS.gene073487.t1.exon4;Parent=MS.gene073487.t1
chr2.4 CDS 53077105 53077155 53077105 ID=cds.MS.gene073487.t1;Parent=MS.gene073487.t1
chr2.4 exon 53076716 53076976 53076716 ID=MS.gene073487.t1.exon5;Parent=MS.gene073487.t1
chr2.4 CDS 53076716 53076976 53076716 ID=cds.MS.gene073487.t1;Parent=MS.gene073487.t1
chr2.4 exon 53076443 53076610 53076443 ID=MS.gene073487.t1.exon6;Parent=MS.gene073487.t1
chr2.4 CDS 53076443 53076610 53076443 ID=cds.MS.gene073487.t1;Parent=MS.gene073487.t1
chr2.4 exon 53076031 53076285 53076031 ID=MS.gene073487.t1.exon7;Parent=MS.gene073487.t1
chr2.4 CDS 53076031 53076285 53076031 ID=cds.MS.gene073487.t1;Parent=MS.gene073487.t1
chr2.4 exon 53074227 53075263 53074227 ID=MS.gene073487.t1.exon8;Parent=MS.gene073487.t1
chr2.4 CDS 53074227 53075263 53074227 ID=cds.MS.gene073487.t1;Parent=MS.gene073487.t1
Gene Sequence

>MS.gene073487

ATGGCTTCGCCGTTAGAGATTGATGAAGAAAATGATAACAAAGGTAGTATGTGGGATTTGGAACAGAAACTTGACCAGCCGATGGATGCCGAAGCAGGAAGGCTCAGAAATATGTACAGAGAAAAAGTATGATGCAAATTCTTTTACTCCATTTTTGCTTTTTCTGAATTCTATTTTGTTTACATAAGATACTGCAATTTGCAACTTTGAAATTTTGATGTCTACAACATGTCTTTTAAGTGATTTTAAGTAATTTTTTTCGATCTATGTTATTTTGATTTGTTTCAGAAGTTTTCTGCGCTGTTGCTTCTACGACTGGCATATCAAAGTCTTGGTGTAGTTTATGGAGATTTGGGAACTTCGCCCTTATACGTTTTCTATAACACATTTCCTCGTGGAGTTGATGATCCGGAGAAAGTGATTGGAGCTCTTTCTTTGATCATATACTCTCTTACTCTTGTGCCGCTGCTCAAATACGTTTTCATTGTATTAAGAGCAAATGACAATGGTCAAGGTATGTATTGTTTTCCAACTGTTTGGTTAATTTGGATATTATGGTTGGACTAAGGAATATTAAGTAATTATTTGATGCAAGAGTAAGAGAGTGGATTTTGACTTAAGCCTTTAGCATTTATTGAATAGGTACTTATTTTCAATCAAGTTTTAAGTTGTTTTTCAGAAGCTATTTTGGAGAGCTTATTGAAATAAGCAAAAATCAGCTTATGAACATATCATAAACTATGTTTATATGTCGTCTCAAACAACTACACTAGTGTTTACGTCATAAGATACACTCAAATAAAGTCATTGTAAATAAGTTATTTCATATGTTGTGTAATTAAGTTTAACCAAGCTTACATCTGTGGAGCAGGTGGAACACTGGCTCTTTATTCCTTGCTTTGCCGACATGCAAATATAAAGATCATTCCGAACCAGCATCGTACTGATGAAGAGTTGACAACATATAGCCGGACTACCTTCCATGAGAAGTCATTTGCTGCCAAAACGCAAAGATGGTTAGAGGAACAAAAATTTATTAAAAGTACCATTCTTATTCTTGTCCTTGTTGGCACCTGCATGGTGATTGGAGACGGTATTCTTACCCCAGCTATATCTGGTATGTTTTCTGTCAGACTCATCTTCTTCCTATTTATTTGTTAGTTCCTCCTTTCATTTTTGACTTTCCTTTTTGTAGAAATTTTTTCAACATATTCATTATGAAATTTTCTGATGTAGTTTTATCTGCTGCTGGTGGCATCAAGGTAAACAAACCCGAAGTTGATAGCGGTATGCTCCTACACTTTTAACATACATTAATCTTTTATGTTTTCCACTATGTATATGAGATTATAGTTTCGAGTTTTTTCATTGGTCTTGAAATTTTTTTTTCCCTTTTTTCCGCCATGGTGTTACAGGAGTAGTAGTGCTGGTTGCTGTTGTAATATTAGTTGGACTATTCAGCATACAACGTTATGGTACAGATAGAGTTGGTTGGCTCTTTGCTCCAATTGTTCTTCTTTGGTTTCTCTTAATTGGAGGTATAGGTATTTACAACTTGTGGAGATTTGGTGGCCGTGTCTTAAAAGCCTTTTCACCTCTCTACGTGTATCGGTATCTAAGAGACGGAGGCAAAGATAGTTGGCTTTCCCTTGGTGGAATCTTGCTTAGCATAACAGGTTTGTTTAATTTTGATAACTTCTGATTTGATTCGTGTGTTTTAAAATTATTTAATAAGGTGAGCGGTTAATTTAAAATCGAAAACTTGTGACGATTGATTTCAGGGACAGAAGCTCTTTTTGCCGACTTAGCCAATTTTCCAGTCTCATCTGTACAAATTGCATTTACTCTCGTGGTGTTTCCTTGCCTTCTTTTAGCATATTCTGGACAGGCTGCATATCTGGTGAATAACTTGCATCATTCACAAGATGTGTTTTACCGTTCTATTCCAGGTTTGTTATATCTGTCACACTACATTAAAATTTCTATTTCTGTTACTTAGATTAGAATGATGCACTTTTGTTTCTTCATCAGGAGCAAAATGTGGGTCTGTCCCTGTCTTCATGCAATGATTTTTATCTGTTAGAGGTTAATTATTCTGTTCTGCAGAAAAAATATACTGGCCGGTATTTGTAATTGCAACGCTAGCAGCAGTAGTTGCAAGCCAAGCCACAATCACCGCAACCTTTTCAATCATTAAGCAAGCCCTTGCTCATGGATGTTTCCCAAGAGTCAAAGTTGTGCATACATCAAAGAAATTCCTCGGCCAGATATATATTCCAGATATCAATTGGATTCTTATGGTTCTATGCATTGCTGTTACTGCTGGTTTTAAAAATCAAACCCAGATTGGAAATGCTTATGGTAAGTTTTTCGCTTGCAGAAGGTTGTGAGCCATTTTTTTCATGTTTTTTTTTTTTAATGAAGAAATAAATTTATTAGAAGGGAGAGAGAAAATGCAACTTGTAGGAGGATATGAAATCATCTAGAAAAATACATTGAAAATTTGCAATATAGGAACTAATCATTACGACATTAAAAAGGGCCTTTCCTAATTTCTTTACATTGAAGGGTCTAATAACTACTAACTTCGTCCCTAGATATAGGACTCGTTGACCAAATCACGGGAGCTAAGAAAGTTGTCAGTAACATCAGATTTGTTGACAACTAGTATTGTTTCGCAAGTTTATCCTTGAGGAGAGATGATAAGTTTATGTTTCCATCATATTATTTAGTACAGGTTGCAGAAAAAAGTGTAACGTAATTAGGGGTATATTGGTACAGAAATAATTAATGCAGCTGAAAATTTAAAGGGGGCCTATAAAACGGGACTGGGAAATAGGATCCTATATTTAGAAACGGAGGGAGCCCTACATATATACATTTTCTCTAACCAATATGCAGTTTATCGTCAACCAGAAATGAACGCTTACTTTGTCATTAGATGATCGTATGTTGTCTATAAAAGTGGGATATATGATGCTCATAATAAAAAAAACTGAAACAGAGTAGCTGAACATAACTAAACGGTTTAAGTGAACCAAGAAGATATACTAGATCATTCACCAGTTAACAAAGTTTAAGAACTTTATTATGCTTATTAACCAGCGAATTTATCCTTGTAATGACAGGCACTGCAGTTGTGTTAGTCATGCTGGTGACAACACTGCTGATGATTTTGGTCATGATATTAGTTTGGCACTGCCATTGGATTCTCGTTGTTGTGTTCACCGGCTTATCATTGATTGTGGAATGCACATATTTTTCGGCTGTACTCTTCAAAATCGATCAAGGTGGCTGGGCTCCCCTTGCAATTGCCGGAGTATTTCTGATTGTTATGTATGTTTGGCATTACGGAACGGTGAAGCGCTACGAGTTTGAATTGCATAGCAAAGTCTCAATGGCATGGATTCTCGGGCTCGGTCCCAGTTTAGGACTAGTCCGTGTCCCTGGAATCGGCCTAGTCTACACAGAGCTTGCAAGTGGAGTTCCCCACATATTTTCTCATTTCATTACAAACCTACCAGCGATCCATTCCGTTGTTGTTTTCGTTTGTGTCAAATATCTTCCTGTCTACACAGTCCCAGAAGAAGAGCGGTTCCTTGTCAAGCGTATTGGACCGAAGAATTACCACATTTTTCGGTGTGTGGCACGATATGGATATAAAGACCTACATAAGAAAGACGATGATTTTGAGAAGAAACTATTTCACAACCTTTTCATGTTTGTTCGGCTCGAGTCCATGATGGAAGGTTGCTCGGATTCAGAATACAGTTTATGTGAACCGCAAACCGAACAGTCGAGAGATTTCATGTTGAATAACAATGGAAACGCAACTTCATTAAGCAATATGGATTATTCAATTTCATCAGTAGATTCAATAGTTCCTGCTAGATCACCAACAAATGTGAATGTTACTTTTCAATCTTCTAACAGTCACTGTACTGAAGTCGATGAACTTGAATTTTTAAACAGCTGTAGAGAAGCTGGTGTTGTTCACATACTAGGAAACACAGTTGTGAGGGCTAGTAGGGATTCAAGATTCTACAAGAAAATAGCTGTTGATTATATATATGCATTTCTTAGGAAGATATGCAGAGAAAATAGTGTGATCTTCAATATTCCTCACGAGAGTCTGTTAAATGTTGGTCAAGTTTTCTATGTATAG
Protein sequence

>MS.gene073487.t1

MASPLEIDEENDNKGSMWDLEQKLDQPMDAEAGRLRNMYREKKFSALLLLRLAYQSLGVVYGDLGTSPLYVFYNTFPRGVDDPEKVIGALSLIIYSLTLVPLLKYVFIVLRANDNGQGGTLALYSLLCRHANIKIIPNQHRTDEELTTYSRTTFHEKSFAAKTQRWLEEQKFIKSTILILVLVGTCMVIGDGILTPAISVLSAAGGIKVNKPEVDSGVVVLVAVVILVGLFSIQRYGTDRVGWLFAPIVLLWFLLIGGIGIYNLWRFGGRVLKAFSPLYVYRYLRDGGKDSWLSLGGILLSITGTEALFADLANFPVSSVQIAFTLVVFPCLLLAYSGQAAYLVNNLHHSQDVFYRSIPEKIYWPVFVIATLAAVVASQATITATFSIIKQALAHGCFPRVKVVHTSKKFLGQIYIPDINWILMVLCIAVTAGFKNQTQIGNAYGTAVVLVMLVTTLLMILVMILVWHCHWILVVVFTGLSLIVECTYFSAVLFKIDQGGWAPLAIAGVFLIVMYVWHYGTVKRYEFELHSKVSMAWILGLGPSLGLVRVPGIGLVYTELASGVPHIFSHFITNLPAIHSVVVFVCVKYLPVYTVPEEERFLVKRIGPKNYHIFRCVARYGYKDLHKKDDDFEKKLFHNLFMFVRLESMMEGCSDSEYSLCEPQTEQSRDFMLNNNGNATSLSNMDYSISSVDSIVPARSPTNVNVTFQSSNSHCTEVDELEFLNSCREAGVVHILGNTVVRASRDSRFYKKIAVDYIYAFLRKICRENSVIFNIPHESLLNVGQVFYV