AlfalfaGEDB | MS.gene08280

M. sativa cultivar XinJiangDaYe / MS.gene08280

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08280.t1	XP_013446502.1	96.1	129	5	0	1	129	1	129	1.20E-63	252.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08280.t1	I3S2Q7	96.1	129	5	0	1	129	1	129	8.8e-64	252.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050277	MS.gene08280	0.811881	5.61E-51	-1.69E-46
MS.gene050916	MS.gene08280	0.812458	4.20E-51	-1.69E-46
MS.gene056075	MS.gene08280	0.803682	3.10E-49	-1.69E-46
MS.gene056631	MS.gene08280	0.812299	4.55E-51	-1.69E-46
MS.gene057094	MS.gene08280	0.800816	1.21E-48	-1.69E-46
MS.gene058906	MS.gene08280	0.818177	2.25E-52	-1.69E-46
MS.gene06001	MS.gene08280	0.833282	5.83E-56	-1.69E-46
MS.gene060429	MS.gene08280	0.802189	6.31E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08280.t1	MTR_8g079498	97.938	97	2	0	16	112	16	112	2.79e-66	197

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08280.t1	AT4G22000	68.376	117	36	1	1	117	1	116	4.73e-54	166

Find 33 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTCTTCATCTCCTTCCTTTC+AGG	0.120263	4.2:+15249310	None:intergenic
CGTAAAGAAGTTCTTCTTGC+TGG	0.300933	4.2:-15249352	MS.gene08280:CDS
TTCTTCTTGCTGGAGAAGAT+TGG	0.378509	4.2:-15249342	MS.gene08280:CDS
AGCTGCTAGTTCAGCGGTAA+AGG	0.432878	4.2:-15249515	MS.gene08280:CDS
GAAAAGGACATAAGGTTGAT+AGG	0.434665	4.2:-15249282	MS.gene08280:CDS
GGCAAACACTGCTAGGTTGA+TGG	0.439655	4.2:-15249242	MS.gene08280:CDS
TTAGTATTAGTGCTCGGCAT+AGG	0.464094	4.2:-15249384	MS.gene08280:CDS
ATGAGTTTGCCAAGGGAGTG+TGG	0.500959	4.2:-15249438	MS.gene08280:CDS
TTGTAAGCAAGCAACATATC+CGG	0.503383	4.2:+15249211	None:intergenic
TAGTATTAGTGCTCGGCATA+GGG	0.503438	4.2:-15249383	MS.gene08280:CDS
TGAGTTTGCCAAGGGAGTGT+GGG	0.513187	4.2:-15249437	MS.gene08280:CDS
GAGATGAAGACGAGGCGAAA+AGG	0.513253	4.2:-15249298	MS.gene08280:CDS
TTTGCTAACTTCTCGCGCCA+TGG	0.520110	4.2:+15249469	None:intergenic
AAGATAAATAGGGTTATGGC+AGG	0.525263	4.2:-15249541	None:intergenic
TGCCGAGCACTAATACTAAG+AGG	0.532897	4.2:+15249388	None:intergenic
GGTATTGGGAGCTAGGGGCA+TGG	0.536258	4.2:-15249414	MS.gene08280:CDS
GAGTTTGCCAAGGGAGTGTG+GGG	0.545739	4.2:-15249436	MS.gene08280:CDS
GACGAGGCGAAAAGGACATA+AGG	0.547643	4.2:-15249290	MS.gene08280:CDS
AGAAGAGGGCAAACACTGCT+AGG	0.550721	4.2:-15249249	MS.gene08280:CDS
AAAGTGGGCGAAGAAGATGA+AGG	0.570139	4.2:-15249182	MS.gene08280:CDS
AAGATGAAGGAAGAAGAGAA+AGG	0.572789	4.2:-15249169	MS.gene08280:CDS
CTACAGAGTATCAATATCCA+TGG	0.573029	4.2:-15249486	MS.gene08280:CDS
CGAGAAGTTAGCAAAATACA+AGG	0.575656	4.2:-15249461	MS.gene08280:CDS
TAGGATAGCTGCGGAGAAGA+GGG	0.580076	4.2:-15249263	MS.gene08280:CDS
AGCCTCTTAGTATTAGTGCT+CGG	0.583490	4.2:-15249390	MS.gene08280:CDS
TAGGTTGATGGATAAGATGC+CGG	0.589131	4.2:-15249230	MS.gene08280:CDS
ATAGGATAGCTGCGGAGAAG+AGG	0.590998	4.2:-15249264	MS.gene08280:CDS
ATACAAGGATGAGTTTGCCA+AGG	0.595001	4.2:-15249446	MS.gene08280:CDS
TACAAGGATGAGTTTGCCAA+GGG	0.634320	4.2:-15249445	MS.gene08280:CDS
TAAGGTTGATAGGATAGCTG+CGG	0.683219	4.2:-15249272	MS.gene08280:CDS
AGGAAAAGCTGCTAGTTCAG+CGG	0.684482	4.2:-15249521	MS.gene08280:CDS
AAAGGAAGGAGATGAAGACG+AGG	0.696241	4.2:-15249306	MS.gene08280:CDS
AGTTTGCCAAGGGAGTGTGG+GGG	0.755828	4.2:-15249435	MS.gene08280:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TGCTTACAAGAAGAAAAAGT+GGG	-	chr4.2:15249488-15249507	MS.gene08280:CDS	30.0%
	TTGCTTACAAGAAGAAAAAG+TGG	-	chr4.2:15249487-15249506	MS.gene08280:CDS	30.0%
	AAGATGAAGGAAGAAGAGAA+AGG	-	chr4.2:15249516-15249535	MS.gene08280:CDS	35.0%
	CGAGAAGTTAGCAAAATACA+AGG	-	chr4.2:15249224-15249243	MS.gene08280:CDS	35.0%
	TTGTAAGCAAGCAACATATC+CGG	+	chr4.2:15249477-15249496	None:intergenic	35.0%
!	ATTGGACTTTTGATCCTGAA+AGG	-	chr4.2:15249361-15249380	MS.gene08280:CDS	35.0%
!	CTACAGAGTATCAATATCCA+TGG	-	chr4.2:15249199-15249218	MS.gene08280:CDS	35.0%
!	GAAAAGGACATAAGGTTGAT+AGG	-	chr4.2:15249403-15249422	MS.gene08280:CDS	35.0%
	AGCCTCTTAGTATTAGTGCT+CGG	-	chr4.2:15249295-15249314	MS.gene08280:CDS	40.0%
	ATACAAGGATGAGTTTGCCA+AGG	-	chr4.2:15249239-15249258	MS.gene08280:CDS	40.0%
	CGTAAAGAAGTTCTTCTTGC+TGG	-	chr4.2:15249333-15249352	MS.gene08280:CDS	40.0%
	TACAAGGATGAGTTTGCCAA+GGG	-	chr4.2:15249240-15249259	MS.gene08280:CDS	40.0%
	TAGTATTAGTGCTCGGCATA+GGG	-	chr4.2:15249302-15249321	MS.gene08280:CDS	40.0%
	TTAGTATTAGTGCTCGGCAT+AGG	-	chr4.2:15249301-15249320	MS.gene08280:CDS	40.0%
	TTCTTCTTGCTGGAGAAGAT+TGG	-	chr4.2:15249343-15249362	MS.gene08280:CDS	40.0%
!	GACTTTTGATCCTGAAAGGA+AGG	-	chr4.2:15249365-15249384	MS.gene08280:CDS	40.0%
!!	TAAGGTTGATAGGATAGCTG+CGG	-	chr4.2:15249413-15249432	MS.gene08280:CDS	40.0%
!!	TAGGTTGATGGATAAGATGC+CGG	-	chr4.2:15249455-15249474	MS.gene08280:CDS	40.0%
	AAAGGAAGGAGATGAAGACG+AGG	-	chr4.2:15249379-15249398	MS.gene08280:CDS	45.0%
	AAAGTGGGCGAAGAAGATGA+AGG	-	chr4.2:15249503-15249522	MS.gene08280:CDS	45.0%
	AGGAAAAGCTGCTAGTTCAG+CGG	-	chr4.2:15249164-15249183	MS.gene08280:CDS	45.0%
	GTCTTCATCTCCTTCCTTTC+AGG	+	chr4.2:15249378-15249397	None:intergenic	45.0%
	TGCCGAGCACTAATACTAAG+AGG	+	chr4.2:15249300-15249319	None:intergenic	45.0%
	AGAAGAGGGCAAACACTGCT+AGG	-	chr4.2:15249436-15249455	MS.gene08280:CDS	50.0%
	AGCTGCTAGTTCAGCGGTAA+AGG	-	chr4.2:15249170-15249189	MS.gene08280:CDS	50.0%
	ATAGGATAGCTGCGGAGAAG+AGG	-	chr4.2:15249421-15249440	MS.gene08280:CDS	50.0%
	ATGAGTTTGCCAAGGGAGTG+TGG	-	chr4.2:15249247-15249266	MS.gene08280:CDS	50.0%
	GACGAGGCGAAAAGGACATA+AGG	-	chr4.2:15249395-15249414	MS.gene08280:CDS	50.0%
	GAGATGAAGACGAGGCGAAA+AGG	-	chr4.2:15249387-15249406	MS.gene08280:CDS	50.0%
	TAGGATAGCTGCGGAGAAGA+GGG	-	chr4.2:15249422-15249441	MS.gene08280:CDS	50.0%
	TTTGCTAACTTCTCGCGCCA+TGG	+	chr4.2:15249219-15249238	None:intergenic	50.0%
!	GGCAAACACTGCTAGGTTGA+TGG	-	chr4.2:15249443-15249462	MS.gene08280:CDS	50.0%
!	TGAGTTTGCCAAGGGAGTGT+GGG	-	chr4.2:15249248-15249267	MS.gene08280:CDS	50.0%
!	AGTTTGCCAAGGGAGTGTGG+GGG	-	chr4.2:15249250-15249269	MS.gene08280:CDS	55.0%
!	GAGTTTGCCAAGGGAGTGTG+GGG	-	chr4.2:15249249-15249268	MS.gene08280:CDS	55.0%
!!	CAAGGGAGTGTGGGGGTATT+GGG	-	chr4.2:15249257-15249276	MS.gene08280:CDS	55.0%
!!	TGTGGGGGTATTGGGAGCTA+GGG	-	chr4.2:15249265-15249284	MS.gene08280:CDS	55.0%
	CCAATACCCCCACACTCCCT+TGG	+	chr4.2:15249259-15249278	None:intergenic	60.0%
!	CCAAGGGAGTGTGGGGGTAT+TGG	-	chr4.2:15249256-15249275	MS.gene08280:CDS	60.0%
!	GGTATTGGGAGCTAGGGGCA+TGG	-	chr4.2:15249271-15249290	MS.gene08280:CDS	60.0%
!!	GTGGGGGTATTGGGAGCTAG+GGG	-	chr4.2:15249266-15249285	MS.gene08280:CDS	60.0%
!!	GTGTGGGGGTATTGGGAGCT+AGG	-	chr4.2:15249264-15249283	MS.gene08280:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	15249159	15249548	15249159	ID=MS.gene08280
chr4.2	mRNA	15249159	15249548	15249159	ID=MS.gene08280.t1;Parent=MS.gene08280
chr4.2	exon	15249159	15249548	15249159	ID=MS.gene08280.t1.exon1;Parent=MS.gene08280.t1
chr4.2	CDS	15249159	15249548	15249159	ID=cds.MS.gene08280.t1;Parent=MS.gene08280.t1

Gene Sequence

>MS.gene08280

ATGGCAGGAAAAGCTGCTAGTTCAGCGGTAAAGGCAATAGCTACAGAGTATCAATATCCATGGCGCGAGAAGTTAGCAAAATACAAGGATGAGTTTGCCAAGGGAGTGTGGGGGTATTGGGAGCTAGGGGCATGGAAGCCTCTTAGTATTAGTGCTCGGCATAGGGCAAGAATTCGTAAAGAAGTTCTTCTTGCTGGAGAAGATTGGACTTTTGATCCTGAAAGGAAGGAGATGAAGACGAGGCGAAAAGGACATAAGGTTGATAGGATAGCTGCGGAGAAGAGGGCAAACACTGCTAGGTTGATGGATAAGATGCCGGATATGTTGCTTGCTTACAAGAAGAAAAAGTGGGCGAAGAAGATGAAGGAAGAAGAGAAAGGCAAATTCTGA

Protein sequence

>MS.gene08280.t1

MAGKAASSAVKAIATEYQYPWREKLAKYKDEFAKGVWGYWELGAWKPLSISARHRARIRKEVLLAGEDWTFDPERKEMKTRRKGHKVDRIAAEKRANTARLMDKMPDMLLAYKKKKWAKKMKEEEKGKF