Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09847.t1 | XP_003625515.1 | 100 | 143 | 0 | 0 | 1 | 143 | 1 | 143 | 4.90E-74 | 287 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09847.t1 | O82201 | 91.5 | 141 | 12 | 0 | 1 | 141 | 1 | 141 | 2.2e-70 | 266.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09847.t1 | G7L0L8 | 100.0 | 143 | 0 | 0 | 1 | 143 | 1 | 143 | 3.6e-74 | 287.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene017498 | MS.gene09847 | PPI |
| MS.gene09847 | MS.gene27073 | PPI |
| MS.gene75781 | MS.gene09847 | PPI |
| MS.gene09847 | MS.gene64216 | PPI |
| MS.gene09847 | MS.gene64013 | PPI |
| MS.gene09847 | MS.gene021005 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09847.t1 | MTR_7g100010 | 100.000 | 143 | 0 | 0 | 1 | 143 | 1 | 143 | 2.71e-104 | 294 |
| MS.gene09847.t1 | MTR_8g102180 | 100.000 | 143 | 0 | 0 | 1 | 143 | 1 | 143 | 2.71e-104 | 294 |
| MS.gene09847.t1 | MTR_5g098940 | 42.754 | 138 | 79 | 0 | 3 | 140 | 2 | 139 | 6.08e-39 | 129 |
| MS.gene09847.t1 | MTR_1g025190 | 46.763 | 139 | 74 | 0 | 3 | 141 | 2 | 140 | 2.03e-38 | 127 |
| MS.gene09847.t1 | MTR_5g087820 | 42.537 | 134 | 77 | 0 | 3 | 136 | 26 | 159 | 4.96e-35 | 120 |
| MS.gene09847.t1 | MTR_8g077600 | 37.838 | 148 | 79 | 3 | 3 | 141 | 2 | 145 | 7.50e-31 | 108 |
| MS.gene09847.t1 | MTR_8g077600 | 36.559 | 93 | 46 | 3 | 3 | 86 | 2 | 90 | 8.93e-12 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09847.t1 | AT2G19790 | 90.845 | 142 | 13 | 0 | 1 | 142 | 1 | 142 | 1.27e-96 | 275 |
| MS.gene09847.t1 | AT1G47830 | 47.482 | 139 | 73 | 0 | 3 | 141 | 2 | 140 | 3.80e-39 | 129 |
| MS.gene09847.t1 | AT2G17380 | 42.446 | 139 | 80 | 0 | 3 | 141 | 2 | 140 | 9.73e-39 | 129 |
| MS.gene09847.t1 | AT4G35410 | 42.446 | 139 | 80 | 0 | 3 | 141 | 2 | 140 | 1.94e-37 | 125 |
| MS.gene09847.t1 | AT3G50860 | 40.278 | 144 | 81 | 1 | 3 | 141 | 2 | 145 | 3.65e-35 | 120 |
| MS.gene09847.t1 | AT3G50860 | 40.278 | 144 | 81 | 1 | 3 | 141 | 2 | 145 | 3.72e-35 | 119 |
| MS.gene09847.t1 | AT4G35410 | 46.392 | 97 | 52 | 0 | 3 | 99 | 2 | 98 | 1.37e-25 | 94.0 |
Find 20 sgRNAs with CRISPR-Local
Find 94 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAGAATGAGCTTGCTATTT+TGG | 0.125124 | 7.1:+7327041 | MS.gene09847:intron |
| GAAACCATGGATCGACATTT+TGG | 0.210891 | 7.1:+7327084 | MS.gene09847:CDS |
| TCTGACTCCTATTCAGCTTA+TGG | 0.236679 | 7.1:+7327391 | MS.gene09847:CDS |
| GGGGATCCGATTCGTGTTAA+TGG | 0.262612 | 7.1:+7325650 | MS.gene09847:CDS |
| TACGAACGATTTCGCCTTCA+AGG | 0.388788 | 7.1:-7325740 | None:intergenic |
| GAAGAACGACGAACCCTTGA+AGG | 0.434936 | 7.1:+7325726 | MS.gene09847:CDS |
| TGTTCACCATTAACACGAAT+CGG | 0.437301 | 7.1:-7325656 | None:intergenic |
| TATCATGTTTCATTTAGAAA+AGG | 0.473226 | 7.1:+7327304 | MS.gene09847:CDS |
| GCAACTACAAAATTGTATAC+AGG | 0.518035 | 7.1:+7326644 | MS.gene09847:CDS |
| ACGAACGATTTCGCCTTCAA+GGG | 0.529007 | 7.1:-7325739 | None:intergenic |
| TTTCTGGTTGGAGTTGATGA+CGG | 0.545713 | 7.1:+7326685 | MS.gene09847:CDS |
| GGTCATGAATGGTTGTATTG+TGG | 0.573456 | 7.1:+7327349 | MS.gene09847:CDS |
| CCTCGCCCGTAATGAAAACC+AGG | 0.578527 | 7.1:+7325767 | MS.gene09847:CDS |
| CCATGGTTTCGACTAAGAGA+TGG | 0.580792 | 7.1:-7327071 | None:intergenic |
| GTTGCCAAAATGTCGATCCA+TGG | 0.581731 | 7.1:-7327088 | None:intergenic |
| GTGTTAATGGTGAACAAACA+AGG | 0.585214 | 7.1:+7325663 | MS.gene09847:CDS |
| TTGGAGGAAATGGTCATGAA+TGG | 0.600198 | 7.1:+7327338 | MS.gene09847:CDS |
| CCATCTCTTAGTCGAAACCA+TGG | 0.604583 | 7.1:+7327071 | MS.gene09847:CDS |
| GGTTGGAGTTGATGACGGCG+AGG | 0.687099 | 7.1:+7326690 | MS.gene09847:CDS |
| TTCGTCGTTCTTCAACAGTG+AGG | 0.697820 | 7.1:-7325716 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTTAATAAAATAAAAAACTT+AGG | + | chr7.1:7326383-7326402 | MS.gene09847:intron | 10.0% |
| !! | ATAAATAATAAACAATGACA+AGG | - | chr7.1:7325861-7325880 | None:intergenic | 15.0% |
| !! | ATGAAAATAAAAGAAAAGAA+TGG | - | chr7.1:7325800-7325819 | None:intergenic | 15.0% |
| !!! | TTGAAATTTTTATCATAGAT+GGG | + | chr7.1:7326250-7326269 | MS.gene09847:intron | 15.0% |
| !!! | TTTGAAATTTTTATCATAGA+TGG | + | chr7.1:7326249-7326268 | MS.gene09847:intron | 15.0% |
| !! | ACAATACTAATAATAACTTC+AGG | - | chr7.1:7326905-7326924 | None:intergenic | 20.0% |
| !! | ATTAGCTAAAATTGAATGTA+AGG | - | chr7.1:7325835-7325854 | None:intergenic | 20.0% |
| !! | CGATATCAATTTATTTACAA+TGG | - | chr7.1:7326968-7326987 | None:intergenic | 20.0% |
| !! | TAAATAAGATATAGATGTCT+TGG | + | chr7.1:7326216-7326235 | MS.gene09847:intron | 20.0% |
| !! | TATCATGTTTCATTTAGAAA+AGG | + | chr7.1:7327304-7327323 | MS.gene09847:CDS | 20.0% |
| !! | TCAAAATTGAAACAAAAACT+AGG | + | chr7.1:7325943-7325962 | MS.gene09847:intron | 20.0% |
| !! | TTTACAACAGAAAATTCATT+CGG | - | chr7.1:7326874-7326893 | None:intergenic | 20.0% |
| !!! | ATTCTAGTTGATTTATACAA+AGG | + | chr7.1:7326737-7326756 | MS.gene09847:intron | 20.0% |
| ! | AATCTATCATGTAGAACTTA+TGG | - | chr7.1:7326063-7326082 | None:intergenic | 25.0% |
| ! | AGAAACAAAAAATCAGTGTT+TGG | - | chr7.1:7327245-7327264 | None:intergenic | 25.0% |
| ! | ATAGTTGACAATAATAACAC+AGG | - | chr7.1:7326363-7326382 | None:intergenic | 25.0% |
| ! | GAAGAAAACAATAGACATTA+TGG | - | chr7.1:7327196-7327215 | None:intergenic | 25.0% |
| ! | GTTAGTTACCAAAAACATAT+AGG | + | chr7.1:7326539-7326558 | MS.gene09847:intron | 25.0% |
| ! | TACTGTATATCAATACAACA+TGG | + | chr7.1:7326426-7326445 | MS.gene09847:intron | 25.0% |
| ! | TAGTTGACAATAATAACACA+GGG | - | chr7.1:7326362-7326381 | None:intergenic | 25.0% |
| ! | TATACAGTAATGAAAGAAAG+CGG | - | chr7.1:7326415-7326434 | None:intergenic | 25.0% |
| !!! | AGAAAAGGTACATTTTATGT+TGG | + | chr7.1:7327319-7327338 | MS.gene09847:CDS | 25.0% |
| AATCATTGACCTAATACCTT+AGG | - | chr7.1:7326452-7326471 | None:intergenic | 30.0% | |
| AATTGATATCGCTTCAAATC+TGG | + | chr7.1:7326977-7326996 | MS.gene09847:intron | 30.0% | |
| AGTTGACAATAATAACACAG+GGG | - | chr7.1:7326361-7326380 | None:intergenic | 30.0% | |
| CTAAAGCCAATTTCAGAATA+TGG | - | chr7.1:7327222-7327241 | None:intergenic | 30.0% | |
| GCAACTACAAAATTGTATAC+AGG | + | chr7.1:7326644-7326663 | MS.gene09847:CDS | 30.0% | |
| TAAGATATAGATGTCTTGGA+AGG | + | chr7.1:7326220-7326239 | MS.gene09847:intron | 30.0% | |
| TTACAATGGAAGTGATACAT+GGG | - | chr7.1:7326954-7326973 | None:intergenic | 30.0% | |
| TTTACAATGGAAGTGATACA+TGG | - | chr7.1:7326955-7326974 | None:intergenic | 30.0% | |
| ! | ACATTTTATGTTGGAGGAAA+TGG | + | chr7.1:7327328-7327347 | MS.gene09847:CDS | 30.0% |
| ! | CAACTGAACCTATATGTTTT+TGG | - | chr7.1:7326550-7326569 | None:intergenic | 30.0% |
| ! | CTAGTTGATTTATACAAAGG+TGG | + | chr7.1:7326740-7326759 | MS.gene09847:intron | 30.0% |
| ! | GTTTTATCCATAAGCTGAAT+AGG | - | chr7.1:7327401-7327420 | None:intergenic | 30.0% |
| !! | AAAGGTACATTTTATGTTGG+AGG | + | chr7.1:7327322-7327341 | MS.gene09847:CDS | 30.0% |
| !! | TTCCTTCCATATTCTGAAAT+TGG | + | chr7.1:7327213-7327232 | MS.gene09847:intron | 30.0% |
| !!! | CTATGCATCTTTGTTTTTTC+TGG | + | chr7.1:7326669-7326688 | MS.gene09847:CDS | 30.0% |
| AGCCAATTTCAGAATATGGA+AGG | - | chr7.1:7327218-7327237 | None:intergenic | 35.0% | |
| ATTGTTCATTCTACCTTCTG+TGG | + | chr7.1:7327010-7327029 | MS.gene09847:intron | 35.0% | |
| CATAAACACTTGTGAGACTA+TGG | + | chr7.1:7325995-7326014 | MS.gene09847:intron | 35.0% | |
| CATAGATGGGAGAAAATAGT+TGG | + | chr7.1:7326263-7326282 | MS.gene09847:intron | 35.0% | |
| GACATTATGGAAGTAATGTG+AGG | - | chr7.1:7327183-7327202 | None:intergenic | 35.0% | |
| GGAAAAGAAAGTAGTTAGTG+AGG | + | chr7.1:7326312-7326331 | MS.gene09847:intron | 35.0% | |
| GTGTTAATGGTGAACAAACA+AGG | + | chr7.1:7325663-7325682 | MS.gene09847:CDS | 35.0% | |
| TATCAATACAACATGGCCTA+AGG | + | chr7.1:7326433-7326452 | MS.gene09847:intron | 35.0% | |
| TCTGCAAAATTATCCACAGA+AGG | - | chr7.1:7327026-7327045 | None:intergenic | 35.0% | |
| TGTTCACCATTAACACGAAT+CGG | - | chr7.1:7325659-7325678 | None:intergenic | 35.0% | |
| ! | CTACTTTCTTTTCCCCAATT+TGG | - | chr7.1:7326306-7326325 | None:intergenic | 35.0% |
| ! | TGGCTGATACATTTTTGTCA+TGG | + | chr7.1:7326598-7326617 | MS.gene09847:intron | 35.0% |
| !! | AGCATACCTGGTTTTCATTA+CGG | - | chr7.1:7325776-7325795 | None:intergenic | 35.0% |
| !!! | GCATCTTTGTTTTTTCTGGT+TGG | + | chr7.1:7326673-7326692 | MS.gene09847:CDS | 35.0% |
| ATTACGCTGAAGCTGAAATG+TGG | - | chr7.1:7326929-7326948 | None:intergenic | 40.0% | |
| CCAAGCATGCACTAAAACTA+GGG | - | chr7.1:7325888-7325907 | None:intergenic | 40.0% | |
| GGTCATGAATGGTTGTATTG+TGG | + | chr7.1:7327349-7327368 | MS.gene09847:CDS | 40.0% | |
| GTCATGGCTTCAAATGTGAT+CGG | + | chr7.1:7326824-7326843 | MS.gene09847:intron | 40.0% | |
| TCCAAGCATGCACTAAAACT+AGG | - | chr7.1:7325889-7325908 | None:intergenic | 40.0% | |
| TCGGTTTCTTTAGACAAGCA+AGG | - | chr7.1:7326855-7326874 | None:intergenic | 40.0% | |
| TCTGACTCCTATTCAGCTTA+TGG | + | chr7.1:7327391-7327410 | MS.gene09847:CDS | 40.0% | |
| TGTATCAGCCAGATCAATCA+AGG | - | chr7.1:7326589-7326608 | None:intergenic | 40.0% | |
| TTGGAGGAAATGGTCATGAA+TGG | + | chr7.1:7327338-7327357 | MS.gene09847:CDS | 40.0% | |
| ! | ACAACATGGCCTAAGGTATT+AGG | + | chr7.1:7326440-7326459 | MS.gene09847:intron | 40.0% |
| ! | CTTTCTTTTCCCCAATTTGG+CGG | - | chr7.1:7326303-7326322 | None:intergenic | 40.0% |
| ! | GAAAAGAATGGAAGCATACC+TGG | - | chr7.1:7325788-7325807 | None:intergenic | 40.0% |
| ! | GAAACCATGGATCGACATTT+TGG | + | chr7.1:7327084-7327103 | MS.gene09847:CDS | 40.0% |
| ! | TTTCTTTTCCCCAATTTGGC+GGG | - | chr7.1:7326302-7326321 | None:intergenic | 40.0% |
| !! | GCATACCTGGTTTTCATTAC+GGG | - | chr7.1:7325775-7325794 | None:intergenic | 40.0% |
| !! | GTTTTGATCTAACTGACCAG+TGG | + | chr7.1:7326802-7326821 | MS.gene09847:intron | 40.0% |
| !! | TTTCTGGTTGGAGTTGATGA+CGG | + | chr7.1:7326685-7326704 | MS.gene09847:CDS | 40.0% |
| !!! | GCAGAATGAGCTTGCTATTT+TGG | + | chr7.1:7327041-7327060 | MS.gene09847:intron | 40.0% |
| ACGAACGATTTCGCCTTCAA+GGG | - | chr7.1:7325742-7325761 | None:intergenic | 45.0% | |
| ATCTAACTGACCAGTGGTCA+TGG | + | chr7.1:7326808-7326827 | MS.gene09847:intron | 45.0% | |
| CCATCTCTTAGTCGAAACCA+TGG | + | chr7.1:7327071-7327090 | MS.gene09847:CDS | 45.0% | |
| CCATGGTTTCGACTAAGAGA+TGG | - | chr7.1:7327074-7327093 | None:intergenic | 45.0% | |
| CTGCTGTGACTAAGAGTCTT+TGG | + | chr7.1:7327121-7327140 | MS.gene09847:intron | 45.0% | |
| GCCAGATCAATCAAGGTGAA+CGG | - | chr7.1:7326582-7326601 | None:intergenic | 45.0% | |
| GTTGCCAAAATGTCGATCCA+TGG | - | chr7.1:7327091-7327110 | None:intergenic | 45.0% | |
| TACGAACGATTTCGCCTTCA+AGG | - | chr7.1:7325743-7325762 | None:intergenic | 45.0% | |
| TCCGTTCACCTTGATTGATC+TGG | + | chr7.1:7326578-7326597 | MS.gene09847:intron | 45.0% | |
| TTCGTCGTTCTTCAACAGTG+AGG | - | chr7.1:7325719-7325738 | None:intergenic | 45.0% | |
| ! | ACATTTGAAGCCATGACCAC+TGG | - | chr7.1:7326821-7326840 | None:intergenic | 45.0% |
| ! | TTCTTTTCCCCAATTTGGCG+GGG | - | chr7.1:7326301-7326320 | None:intergenic | 45.0% |
| !!! | CCCTAGTTTTAGTGCATGCT+TGG | + | chr7.1:7325885-7325904 | MS.gene09847:intron | 45.0% |
| !! | TTAATAAAATAAAAAACTTA+GGG | + | chr7.1:7326384-7326403 | MS.gene09847:intron | 5.0% |
| CTTTCACACCCCGCCAAATT+GGG | + | chr7.1:7326290-7326309 | MS.gene09847:intron | 50.0% | |
| GAAGAACGACGAACCCTTGA+AGG | + | chr7.1:7325726-7325745 | MS.gene09847:CDS | 50.0% | |
| GTGCATGCTTGGATCATCGA+AGG | + | chr7.1:7325896-7325915 | MS.gene09847:intron | 50.0% | |
| TTTCACACCCCGCCAAATTG+GGG | + | chr7.1:7326291-7326310 | MS.gene09847:intron | 50.0% | |
| ! | GGGGATCCGATTCGTGTTAA+TGG | + | chr7.1:7325650-7325669 | MS.gene09847:CDS | 50.0% |
| !! | GGATCGACATTTTGGCAACG+TGG | + | chr7.1:7327092-7327111 | MS.gene09847:CDS | 50.0% |
| CCAATTTGGCGGGGTGTGAA+AGG | - | chr7.1:7326292-7326311 | None:intergenic | 55.0% | |
| CCTCGCCCGTAATGAAAACC+AGG | + | chr7.1:7325767-7325786 | MS.gene09847:CDS | 55.0% | |
| CCTTTCACACCCCGCCAAAT+TGG | + | chr7.1:7326289-7326308 | MS.gene09847:intron | 55.0% | |
| !! | CCTGGTTTTCATTACGGGCG+AGG | - | chr7.1:7325770-7325789 | None:intergenic | 55.0% |
| !! | GGTTGGAGTTGATGACGGCG+AGG | + | chr7.1:7326690-7326709 | MS.gene09847:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 7325648 | 7327427 | 7325648 | ID=MS.gene09847 |
| chr7.1 | mRNA | 7325648 | 7327427 | 7325648 | ID=MS.gene09847.t1;Parent=MS.gene09847 |
| chr7.1 | exon | 7325648 | 7325788 | 7325648 | ID=MS.gene09847.t1.exon1;Parent=MS.gene09847.t1 |
| chr7.1 | CDS | 7325648 | 7325788 | 7325648 | ID=cds.MS.gene09847.t1;Parent=MS.gene09847.t1 |
| chr7.1 | exon | 7326625 | 7326711 | 7326625 | ID=MS.gene09847.t1.exon2;Parent=MS.gene09847.t1 |
| chr7.1 | CDS | 7326625 | 7326711 | 7326625 | ID=cds.MS.gene09847.t1;Parent=MS.gene09847.t1 |
| chr7.1 | exon | 7327045 | 7327113 | 7327045 | ID=MS.gene09847.t1.exon3;Parent=MS.gene09847.t1 |
| chr7.1 | CDS | 7327045 | 7327113 | 7327045 | ID=cds.MS.gene09847.t1;Parent=MS.gene09847.t1 |
| chr7.1 | exon | 7327293 | 7327427 | 7327293 | ID=MS.gene09847.t1.exon4;Parent=MS.gene09847.t1 |
| chr7.1 | CDS | 7327293 | 7327427 | 7327293 | ID=cds.MS.gene09847.t1;Parent=MS.gene09847.t1 |
| Gene Sequence |
| Protein sequence |
