AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20195

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20195.t1 MTR_8g100075 99.046 629 6 0 1 629 1 629 0.0 1305
MS.gene20195.t1 MTR_0019s0120 56.390 626 262 5 9 628 14 634 0.0 773
MS.gene20195.t1 MTR_7g076170 60.032 618 224 6 20 622 18 627 0.0 771
MS.gene20195.t1 MTR_8g432680 57.800 609 249 5 22 622 24 632 0.0 751
MS.gene20195.t1 MTR_1g101370 57.167 600 243 6 28 621 27 618 0.0 746
MS.gene20195.t1 MTR_4g098890 56.551 603 232 7 25 617 18 600 0.0 729
MS.gene20195.t1 MTR_8g055840 54.023 609 258 5 25 628 17 608 0.0 710
MS.gene20195.t1 MTR_5g040360 52.979 621 263 7 17 629 9 608 0.0 693
MS.gene20195.t1 MTR_3g040370 52.451 612 258 6 25 628 19 605 0.0 666
MS.gene20195.t1 MTR_0019s0120 56.084 526 220 5 9 528 14 534 0.0 644
MS.gene20195.t1 MTR_7g006060 49.815 540 265 3 81 614 26 565 0.0 601
MS.gene20195.t1 MTR_8g071280 44.677 573 303 10 61 627 31 595 1.93e-175 511
MS.gene20195.t1 MTR_5g026930 46.768 526 270 7 98 620 72 590 5.50e-173 505
MS.gene20195.t1 MTR_7g076150 43.805 573 282 10 63 620 40 587 3.36e-172 503
MS.gene20195.t1 MTR_8g432620 42.328 567 297 8 63 621 57 601 8.12e-170 497
MS.gene20195.t1 MTR_1g442840 45.825 515 249 11 96 596 90 588 1.99e-155 461
MS.gene20195.t1 MTR_5g080720 42.222 540 271 11 95 617 122 637 3.09e-154 459
MS.gene20195.t1 MTR_1g442840 46.516 488 235 10 119 596 4 475 1.63e-151 447
MS.gene20195.t1 MTR_1g069785 41.681 571 293 14 73 620 32 585 1.09e-149 446
MS.gene20195.t1 MTR_1g075900 42.572 552 280 14 96 627 89 623 2.40e-147 441
MS.gene20195.t1 MTR_3g068990 41.744 539 289 10 88 617 143 665 4.61e-147 441
MS.gene20195.t1 MTR_7g076150 45.923 466 218 8 167 620 1 444 8.29e-147 432
MS.gene20195.t1 MTR_1g029730 40.602 532 283 11 97 619 151 658 1.33e-142 429
MS.gene20195.t1 MTR_5g026930 46.774 434 222 6 98 528 72 499 1.54e-141 421
MS.gene20195.t1 MTR_3g096480 40.489 573 302 16 63 620 228 776 8.80e-138 421
MS.gene20195.t1 MTR_5g080720 42.500 480 235 11 95 557 122 577 2.32e-134 405
MS.gene20195.t1 MTR_3g088790 41.045 536 281 15 101 620 226 742 2.52e-133 409
MS.gene20195.t1 MTR_8g093880 40.182 550 294 16 63 596 256 786 1.45e-132 409
MS.gene20195.t1 MTR_5g083150 39.810 525 278 15 107 620 101 598 5.36e-130 395
MS.gene20195.t1 MTR_1g051055 36.305 617 342 12 25 621 19 604 5.82e-130 395
MS.gene20195.t1 MTR_5g021100 38.951 534 290 14 101 620 322 833 3.67e-129 400
MS.gene20195.t1 MTR_3g071530 38.388 521 287 8 107 620 153 646 2.36e-128 392
MS.gene20195.t1 MTR_8g076650 38.752 529 297 11 101 620 380 890 2.39e-124 389
MS.gene20195.t1 MTR_1g022255 34.981 526 293 15 108 620 210 699 2.26e-97 313
MS.gene20195.t1 MTR_4g083030 32.613 555 328 15 87 619 73 603 6.11e-96 307
MS.gene20195.t1 MTR_3g074780 32.155 566 316 16 95 620 151 688 2.02e-94 305
MS.gene20195.t1 MTR_5g064080 31.429 560 324 16 96 624 144 674 6.15e-92 298
MS.gene20195.t1 MTR_1g022255 29.517 393 234 12 108 488 210 571 2.45e-50 184
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20195.t1 AT1G33170 71.520 625 160 4 16 622 12 636 0.0 959
MS.gene20195.t1 AT4G10440 70.906 629 173 5 1 626 1 622 0.0 939
MS.gene20195.t1 AT2G45750 58.576 618 241 6 17 626 11 621 0.0 761
MS.gene20195.t1 AT4G00750 55.217 623 265 5 15 629 15 631 0.0 738
MS.gene20195.t1 AT1G26850 55.354 607 246 5 25 621 18 609 0.0 712
MS.gene20195.t1 AT1G26850 55.354 607 246 5 25 621 18 609 0.0 712
MS.gene20195.t1 AT4G18030 52.674 617 272 8 12 623 5 606 0.0 677
MS.gene20195.t1 AT2G43200 50.860 523 251 4 98 618 91 609 0.0 595
MS.gene20195.t1 AT2G43200 50.860 523 251 4 98 618 91 609 0.0 595
MS.gene20195.t1 AT1G26850 54.600 500 202 5 25 514 18 502 0.0 565
MS.gene20195.t1 AT1G31850 47.917 528 263 8 98 620 76 596 0.0 539
MS.gene20195.t1 AT1G31850 47.917 528 263 8 98 620 76 596 0.0 539
MS.gene20195.t1 AT1G31850 47.917 528 263 8 98 620 76 596 0.0 539
MS.gene20195.t1 AT1G31850 47.917 528 263 8 98 620 76 596 0.0 539
MS.gene20195.t1 AT4G19120 46.715 548 274 9 82 620 52 590 0.0 530
MS.gene20195.t1 AT4G19120 46.715 548 274 9 82 620 52 590 0.0 530
MS.gene20195.t1 AT4G00740 43.182 528 274 7 101 620 84 593 1.18e-158 469
MS.gene20195.t1 AT4G00740 43.182 528 274 7 101 620 84 593 1.18e-158 469
MS.gene20195.t1 AT5G06050 40.422 569 295 12 71 619 119 663 2.57e-154 460
MS.gene20195.t1 AT4G19120 46.930 456 225 8 82 528 52 499 1.70e-150 444
MS.gene20195.t1 AT2G39750 39.831 590 306 13 52 617 125 689 1.64e-149 448
MS.gene20195.t1 AT1G77260 41.917 532 282 10 95 617 140 653 1.27e-147 442
MS.gene20195.t1 AT1G04430 42.336 548 277 12 96 620 82 613 3.91e-146 437
MS.gene20195.t1 AT1G04430 42.336 548 277 12 96 620 82 613 3.91e-146 437
MS.gene20195.t1 AT1G04430 42.336 548 277 12 96 620 82 613 3.91e-146 437
MS.gene20195.t1 AT4G14360 43.883 515 259 12 96 596 74 572 1.02e-145 436
MS.gene20195.t1 AT4G14360 43.883 515 259 12 96 596 74 572 1.02e-145 436
MS.gene20195.t1 AT3G23300 42.144 541 281 13 96 620 77 601 4.95e-144 431
MS.gene20195.t1 AT3G23300 42.144 541 281 13 96 620 77 601 4.95e-144 431
MS.gene20195.t1 AT5G14430 42.647 544 278 14 96 621 78 605 1.94e-139 420
MS.gene20195.t1 AT5G14430 42.279 544 280 14 96 621 78 605 1.84e-138 417
MS.gene20195.t1 AT2G34300 40.208 577 307 16 58 620 205 757 3.86e-136 416
MS.gene20195.t1 AT2G34300 40.208 577 307 16 58 620 205 757 3.86e-136 416
MS.gene20195.t1 AT2G34300 40.208 577 307 16 58 620 205 757 3.86e-136 416
MS.gene20195.t1 AT1G29470 42.135 534 276 15 101 620 243 757 2.16e-135 414
MS.gene20195.t1 AT1G29470 42.135 534 276 15 101 620 243 757 2.16e-135 414
MS.gene20195.t1 AT2G40280 40.571 525 268 13 107 620 90 581 1.03e-133 404
MS.gene20195.t1 AT5G64030 39.062 576 308 16 63 620 266 816 9.42e-132 407
MS.gene20195.t1 AT3G51070 40.706 538 279 16 101 620 374 889 4.43e-130 404
MS.gene20195.t1 AT3G56080 39.734 526 274 15 107 620 114 608 3.87e-129 393
MS.gene20195.t1 AT3G10200 37.313 536 300 9 101 621 75 589 9.10e-129 392
MS.gene20195.t1 AT5G04060 36.517 534 305 8 101 621 86 598 7.21e-127 387
MS.gene20195.t1 AT3G10200 39.175 485 260 8 101 571 75 538 1.04e-122 376
MS.gene20195.t1 AT3G51070 42.766 470 237 14 101 554 374 827 3.32e-116 366
MS.gene20195.t1 AT3G56080 41.087 460 236 14 107 554 114 550 1.20e-114 354
MS.gene20195.t1 AT1G19430 35.038 528 291 17 108 620 232 722 2.81e-95 308
MS.gene20195.t1 AT1G19430 35.038 528 291 17 108 620 232 722 2.81e-95 308
MS.gene20195.t1 AT1G13860 32.613 555 308 15 95 617 78 598 1.81e-93 300
MS.gene20195.t1 AT1G13860 32.613 555 308 15 95 617 78 598 1.81e-93 300
MS.gene20195.t1 AT1G13860 32.613 555 308 15 95 617 78 598 1.81e-93 300
MS.gene20195.t1 AT1G13860 32.613 555 308 15 95 617 78 598 1.81e-93 300
MS.gene20195.t1 AT2G03480 31.119 572 319 14 77 619 67 592 2.38e-90 292
MS.gene20195.t1 AT2G03480 31.607 560 343 13 77 619 67 603 7.74e-89 288
MS.gene20195.t1 AT1G78240 31.004 558 328 17 96 619 144 678 2.16e-87 286
MS.gene20195.t1 AT1G78240 31.004 558 328 17 96 619 144 678 2.16e-87 286
MS.gene20195.t1 AT1G13860 33.693 463 255 13 184 617 3 442 9.83e-82 265
MS.gene20195.t1 AT1G19430 31.313 396 226 14 108 489 232 595 1.15e-53 194
MS.gene20195.t1 AT2G03480 30.792 341 217 6 77 408 67 397 9.46e-52 184

Find 139 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGGTTATTGGATTCTTTC+TGG 0.100373 4.4:-4303652 MS.gene20195:CDS
TTAAAAGGTGTCTTATATTT+TGG 0.133343 4.4:+4304484 None:intergenic
TTGAGAAAGCTGTTCAAAAT+TGG 0.200150 4.4:-4304402 MS.gene20195:CDS
ATTCATGCAGCTGGTGTATT+TGG 0.210043 4.4:-4302818 MS.gene20195:CDS
TCCTTAGGCCAGGTGGTTAT+TGG 0.243011 4.4:-4303663 MS.gene20195:CDS
CTACTTTGAGGCCATTTAAA+AGG 0.243262 4.4:+4304469 None:intergenic
CTCATTCCATGGAGCAAATA+TGG 0.258315 4.4:-4303908 MS.gene20195:intron
TTCAAAAGGACAATGAATTA+TGG 0.259353 4.4:-4303090 MS.gene20195:CDS
CTATATCTTCCTTGAGCAAT+AGG 0.274060 4.4:+4303034 None:intergenic
GCGTTTGGCAACTTCCTCTA+TGG 0.300697 4.4:+4303555 None:intergenic
CATTATTAAAATTTCCAGTT+TGG 0.305235 4.4:-4302967 MS.gene20195:CDS
ACAGAAACATGTTGAAATAT+AGG 0.306859 4.4:-4304561 MS.gene20195:CDS
CAACATTACTGTTATACTAT+TGG 0.313122 4.4:-4302583 MS.gene20195:CDS
TCACTTGAAATAAGTACTTC+TGG 0.335289 4.4:-4304667 MS.gene20195:CDS
GGAAGAAATACTGGAGGGGC+TGG 0.344229 4.4:-4303615 MS.gene20195:CDS
ATTGAACCACTACTAATTCT+AGG 0.344619 4.4:+4303136 None:intergenic
ATTATTAAAATTTCCAGTTT+GGG 0.346106 4.4:-4302966 MS.gene20195:CDS
CAGTATTTCTTCCATCTAAT+AGG 0.347198 4.4:+4303625 None:intergenic
AATTATAAAGGTAGCAAGTT+GGG 0.358008 4.4:-4304107 MS.gene20195:intron
CCATGTGAGTCTATTTCTCT+TGG 0.358721 4.4:+4304873 None:intergenic
ATCTTTGAAAGAGGTTTCAT+TGG 0.366006 4.4:-4302896 MS.gene20195:CDS
TTTCTGGCCCACCTATTAGA+TGG 0.370798 4.4:-4303636 MS.gene20195:CDS
TGGTGATCCATTGTAATCCT+TGG 0.371322 4.4:+4304918 None:intergenic
TCAAGCTGATTATAATGCTT+TGG 0.372036 4.4:+4304898 None:intergenic
CAGAAACATGTTGAAATATA+GGG 0.373112 4.4:-4304560 MS.gene20195:CDS
TTGCTTGAGCTGGTAACTTC+TGG 0.378404 4.4:+4303211 None:intergenic
GGTGATCCATTGTAATCCTT+GGG 0.378516 4.4:+4304919 None:intergenic
GCCATATCAAAAGCCCTTGC+TGG 0.383647 4.4:+4303947 None:intergenic
CTATTAGATGGAAGAAATAC+TGG 0.384247 4.4:-4303624 MS.gene20195:CDS
ATCAGAACTGCAATTGATAC+AGG 0.390635 4.4:-4304265 MS.gene20195:CDS
TGGTGTATTTGGCATATATC+AGG 0.393487 4.4:-4302807 MS.gene20195:CDS
TCCTGCTATGATTGGTATCA+TGG 0.402427 4.4:-4303990 MS.gene20195:CDS
ACAACATTCATAACCCAAAC+TGG 0.404193 4.4:+4302953 None:intergenic
TGAATGAGATCATAAGTTCT+AGG 0.405756 4.4:+4302836 None:intergenic
TCATTGGGACTTACCATGAT+TGG 0.417773 4.4:-4302880 MS.gene20195:CDS
TCCAGCTACTTTGCTTGAGC+TGG 0.418390 4.4:+4303201 None:intergenic
ATTGATATGAATGCTTATCT+TGG 0.425704 4.4:-4303004 MS.gene20195:CDS
TTTCTTCCAACATATGCGTT+TGG 0.430721 4.4:+4303540 None:intergenic
TATGCCTCAGATTGGTTAGT+AGG 0.432612 4.4:+4304784 None:intergenic
GTTTAGTTTGTACACATTCA+AGG 0.437945 4.4:+4303470 None:intergenic
GTTCTCGCTGCCTCATTCCA+TGG 0.445475 4.4:-4303919 MS.gene20195:CDS
CAAAATTGGATTCAAGTTGA+AGG 0.446164 4.4:-4304388 MS.gene20195:CDS
AGAAAGATGACCTTTCCATT+TGG 0.448933 4.4:-4303510 MS.gene20195:CDS
TCAAAAGTAGATTGTAATGT+TGG 0.460424 4.4:-4304760 MS.gene20195:CDS
AATTCAGCTCATGATACTCT+TGG 0.465587 4.4:-4302923 MS.gene20195:CDS
CAAGCATAATCTCTACTTTG+AGG 0.466308 4.4:+4304457 None:intergenic
GAGCTTATTCCTCTTACTAC+TGG 0.466754 4.4:-4304292 MS.gene20195:CDS
CCCAGAAAAGATTCTTGTTG+CGG 0.467901 4.4:-4302424 MS.gene20195:CDS
TCTTTGAAAGAGGTTTCATT+GGG 0.470943 4.4:-4302895 MS.gene20195:CDS
CTGATATTACCAGTAGTAAG+AGG 0.472181 4.4:+4304283 None:intergenic
GGTGATCGGTTTCGATTCCC+TGG 0.472490 4.4:-4304367 MS.gene20195:CDS
GTATCATGAGCTGAATTTGA+AGG 0.474584 4.4:+4302929 None:intergenic
GAAGTGGAAGAGCAACATAA+TGG 0.475548 4.4:-4302469 MS.gene20195:CDS
TCCAATAACCACCTGGCCTA+AGG 0.482017 4.4:+4303662 None:intergenic
CTTGGTGCAATCTTTGAAAG+AGG 0.483757 4.4:-4302905 MS.gene20195:CDS
AGAAAGCTTCACACCAATCA+TGG 0.487974 4.4:+4302867 None:intergenic
TCAAAAGCCCTTGCTGGGTA+TGG 0.491448 4.4:+4303953 None:intergenic
ACCAGCTCAAGCAAAGTAGC+TGG 0.491717 4.4:-4303202 MS.gene20195:CDS
AATACTTACCAAGCCATGTC+AGG 0.492107 4.4:+4303409 None:intergenic
AGAGGAGTTCCTGCTATGAT+TGG 0.493820 4.4:-4303998 MS.gene20195:CDS
GATATGAATGCTTATCTTGG+TGG 0.494234 4.4:-4303001 MS.gene20195:CDS
CTGATAATCCTGACATGGCT+TGG 0.495727 4.4:-4303417 MS.gene20195:intron
TACTGTTATACTATTGGAGA+TGG 0.501641 4.4:-4302577 MS.gene20195:CDS
GGATTCAAGTTGAAGGTGAT+CGG 0.505103 4.4:-4304381 MS.gene20195:CDS
GAAGAAATACTGGAGGGGCT+GGG 0.506084 4.4:-4303614 MS.gene20195:CDS
TTTCCATGCACCCATTACAT+AGG 0.506215 4.4:+4304819 None:intergenic
AGCTCAGGTACAGTTTGCAT+TGG 0.506264 4.4:-4304023 MS.gene20195:CDS
TAATTATAAAGGTAGCAAGT+TGG 0.506609 4.4:-4304108 MS.gene20195:intron
CGGTTTCGATTCCCTGGTGG+AGG 0.508041 4.4:-4304361 MS.gene20195:CDS
TGAGCTTCATGTGTATCTCT+TGG 0.511536 4.4:+4304040 None:intergenic
AATTTCTCTGCAGTGATGCT+TGG 0.514766 4.4:+4303112 None:intergenic
TATGATCTCATTCATGCAGC+TGG 0.517745 4.4:-4302827 MS.gene20195:CDS
GAGCTGGTAACTTCTGGTAA+TGG 0.518242 4.4:+4303217 None:intergenic
CTGAATACAACTGTACCTTC+TGG 0.521917 4.4:+4302540 None:intergenic
CTCAAAGTAGAGATTATGCT+TGG 0.522721 4.4:-4304456 MS.gene20195:CDS
TGTAATGTTGGATCAACAGC+AGG 0.524580 4.4:-4304748 MS.gene20195:CDS
CGAGGACGATGATGAAATTG+AGG 0.528468 4.4:+4304720 None:intergenic
CACTGCAGAGAAATTTCAAA+AGG 0.531156 4.4:-4303104 MS.gene20195:CDS
TATTTCTTCCATCTAATAGG+TGG 0.536838 4.4:+4303628 None:intergenic
CCAGTCTGATAATCCTGACA+TGG 0.541896 4.4:-4303422 MS.gene20195:CDS
GGTACTGCAAAGGCACGTCT+TGG 0.542582 4.4:+4303160 None:intergenic
AAGAGATACACATGAAGCTC+AGG 0.543833 4.4:-4304038 MS.gene20195:CDS
CATATTTGCTCCATGGAATG+AGG 0.545022 4.4:+4303909 None:intergenic
GGAAACATGGTACCTCCACC+AGG 0.545167 4.4:+4304349 None:intergenic
CCATGTCAGGATTATCAGAC+TGG 0.545882 4.4:+4303422 None:intergenic
CCTCTAGCTGGATCTTGACA+TGG 0.546211 4.4:+4304595 None:intergenic
TTGAAGTGGATAGAGTCCTT+AGG 0.548105 4.4:-4303678 MS.gene20195:CDS
CCATATCAAAAGCCCTTGCT+GGG 0.549809 4.4:+4303948 None:intergenic
CATATCACTCCTATTGCTCA+AGG 0.550740 4.4:-4303043 MS.gene20195:CDS
GTGGATAGAGTCCTTAGGCC+AGG 0.552453 4.4:-4303673 MS.gene20195:CDS
CTCAAGCAAAGTAGCTGGAG+GGG 0.557038 4.4:-4303197 MS.gene20195:CDS
ATCAGCACCGCGAGGAAACA+TGG 0.563999 4.4:+4304336 None:intergenic
TACCAACCATATTTGCTCCA+TGG 0.566081 4.4:+4303902 None:intergenic
TGGTATGTATTTGATTGAAG+TGG 0.568021 4.4:-4303692 MS.gene20195:CDS
TATTTGGCATATATCAGGAT+AGG 0.572492 4.4:-4302802 MS.gene20195:intron
AACATAATGGATCATGAAAG+TGG 0.574348 4.4:-4302456 MS.gene20195:CDS
GAATGAGATCATAAGTTCTA+GGG 0.575775 4.4:+4302837 None:intergenic
GGTACAGTTGTATTCAGAGA+AGG 0.578876 4.4:-4302534 MS.gene20195:CDS
AAAGGCTACCATACCCAGCA+AGG 0.579111 4.4:-4303961 MS.gene20195:CDS
GCCATGATACCAATCATAGC+AGG 0.580506 4.4:+4303989 None:intergenic
ATTTCTTCCATCTAATAGGT+GGG 0.583039 4.4:+4303629 None:intergenic
GATAGAATCTTGAGGCCAGA+AGG 0.587910 4.4:-4302555 MS.gene20195:CDS
CCAAGAGAAATAGACTCACA+TGG 0.591381 4.4:-4304873 MS.gene20195:CDS
TTGAAATAAGTACTTCTGGT+GGG 0.591390 4.4:-4304663 MS.gene20195:CDS
ATTATAAAGGTAGCAAGTTG+GGG 0.592087 4.4:-4304106 MS.gene20195:intron
AAGTTGCCAAACGCATATGT+TGG 0.595240 4.4:-4303546 MS.gene20195:CDS
TTATCAGACTGGCAAATATG+TGG 0.598326 4.4:+4303433 None:intergenic
GTACAGTTTGCATTGGAGAG+AGG 0.599309 4.4:-4304016 MS.gene20195:CDS
AAAGGACAATGAATTATGGA+AGG 0.600355 4.4:-4303086 MS.gene20195:CDS
CTTGAAATAAGTACTTCTGG+TGG 0.600492 4.4:-4304664 MS.gene20195:CDS
GATCGGTTTCGATTCCCTGG+TGG 0.605900 4.4:-4304364 MS.gene20195:CDS
GCTCAAGCAAAGTAGCTGGA+GGG 0.607755 4.4:-4303198 MS.gene20195:CDS
AGCTCAAGCAAAGTAGCTGG+AGG 0.608310 4.4:-4303199 MS.gene20195:CDS
GCAAGAGCAAGATTCCATAG+AGG 0.609694 4.4:-4303569 MS.gene20195:CDS
TCAATGCTAAGCTCTTTGTG+TGG 0.613939 4.4:+4304421 None:intergenic
TGTCCTATGTAATGGGTGCA+TGG 0.614566 4.4:-4304822 MS.gene20195:CDS
GTGTTACTGATGCAATGAAG+TGG 0.625030 4.4:-4302485 MS.gene20195:CDS
GGGCTGGGAAAGAACTGAAG+AGG 0.627289 4.4:-4303599 MS.gene20195:CDS
TAGATGGAAGAAATACTGGA+GGG 0.629603 4.4:-4303620 MS.gene20195:CDS
TCACTATATGTCATGTCACA+TGG 0.634015 4.4:+4304625 None:intergenic
AGATGGAAGAAATACTGGAG+GGG 0.651671 4.4:-4303619 MS.gene20195:CDS
GAAAGAATCCAATAACCACC+TGG 0.654203 4.4:+4303655 None:intergenic
AAAATGCCCAAGGATTACAA+TGG 0.656503 4.4:-4304925 MS.gene20195:CDS
TCAGAACTGCAATTGATACA+GGG 0.660831 4.4:-4304264 MS.gene20195:CDS
AACACCTACTAACCAATCTG+AGG 0.662097 4.4:-4304788 MS.gene20195:CDS
TGGAGATGGATAGAATCTTG+AGG 0.664431 4.4:-4302563 MS.gene20195:CDS
CCATGTCAAGATCCAGCTAG+AGG 0.667824 4.4:-4304595 MS.gene20195:CDS
TTAGATGGAAGAAATACTGG+AGG 0.668874 4.4:-4303621 MS.gene20195:CDS
GTACCTCCTAGAATTAGTAG+TGG 0.671372 4.4:-4303142 MS.gene20195:CDS
AAGGCTACCATACCCAGCAA+GGG 0.674658 4.4:-4303960 MS.gene20195:CDS
ACTGCAATTGATACAGGGTG+TGG 0.682693 4.4:-4304259 MS.gene20195:CDS
AATTCTAGGAGGTACTGCAA+AGG 0.682768 4.4:+4303150 None:intergenic
GAACCACTACTAATTCTAGG+AGG 0.691410 4.4:+4303139 None:intergenic
CTATATGTCATGTCACATGG+TGG 0.691797 4.4:+4304628 None:intergenic
TTGGTATCATGGCTTCACAA+AGG 0.696029 4.4:-4303979 MS.gene20195:CDS
GATAGAGTCCTTAGGCCAGG+TGG 0.700562 4.4:-4303670 MS.gene20195:CDS
GGAGGTACCATGTTTCCTCG+CGG 0.714635 4.4:-4304343 MS.gene20195:CDS
GAAACATGGTACCTCCACCA+GGG 0.720834 4.4:+4304350 None:intergenic
ATGTACGCATCAGCACCGCG+AGG 0.786908 4.4:+4304328 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATTAAAATTTCCAGTTT+GGG - chr4.4:4304336-4304355 MS.gene20195:CDS 15.0%
!!! ATTTAACCTTGTAATTTTAA+AGG + chr4.4:4303925-4303944 None:intergenic 15.0%
!!! TTTAACCTTGTAATTTTAAA+GGG + chr4.4:4303924-4303943 None:intergenic 15.0%
!! AATTAATTGTTATGTATGGA+AGG - chr4.4:4304694-4304713 MS.gene20195:CDS 20.0%
!! ACTGAATTAATTGTTATGTA+TGG - chr4.4:4304690-4304709 MS.gene20195:CDS 20.0%
!! CAAATTCAAATCAAATGTTA+GGG - chr4.4:4303128-4303147 MS.gene20195:CDS 20.0%
!! CATTATTAAAATTTCCAGTT+TGG - chr4.4:4304335-4304354 MS.gene20195:CDS 20.0%
!! TACATGATATTATAAGTTGT+TGG - chr4.4:4304567-4304586 MS.gene20195:CDS 20.0%
!! TCAAATTCAAATCAAATGTT+AGG - chr4.4:4303127-4303146 MS.gene20195:CDS 20.0%
!! TCATGTGAATCTAATTATAA+AGG - chr4.4:4303183-4303202 MS.gene20195:CDS 20.0%
!! TTATAATTAGATTCACATGA+AGG + chr4.4:4303183-4303202 None:intergenic 20.0%
!!! AATATAAGACACCTTTTAAA+TGG - chr4.4:4302822-4302841 MS.gene20195:CDS 20.0%
!!! ATCAAATTTTAAACATGCAA+AGG + chr4.4:4303992-4304011 None:intergenic 20.0%
!!! ATTAAAAAGGTACCAAAATA+TGG - chr4.4:4304048-4304067 MS.gene20195:CDS 20.0%
!!! ATTAGTTTATTACTTATGGT+CGG + chr4.4:4303559-4303578 None:intergenic 20.0%
!!! GCATATTAGTTTATTACTTA+TGG + chr4.4:4303563-4303582 None:intergenic 20.0%
!!! TACCAAAACATATTTTTATG+AGG + chr4.4:4303969-4303988 None:intergenic 20.0%
!!! TGTTTGATTTGCAATTAAAA+AGG - chr4.4:4304035-4304054 MS.gene20195:CDS 20.0%
!!! TTAAAAGGTGTCTTATATTT+TGG + chr4.4:4302821-4302840 None:intergenic 20.0%
!!! TTTTTATTTGCTACACAATT+TGG - chr4.4:4303441-4303460 MS.gene20195:CDS 20.0%
! AATTATAAAGGTAGCAAGTT+GGG - chr4.4:4303195-4303214 MS.gene20195:CDS 25.0%
! ACAGAAACATGTTGAAATAT+AGG - chr4.4:4302741-4302760 MS.gene20195:intron 25.0%
! ATTGAAAATCATCTATCAGT+TGG - chr4.4:4304634-4304653 MS.gene20195:CDS 25.0%
! ATTGATATGAATGCTTATCT+TGG - chr4.4:4304298-4304317 MS.gene20195:CDS 25.0%
! CAACATTACTGTTATACTAT+TGG - chr4.4:4304719-4304738 MS.gene20195:CDS 25.0%
! CAGAAACATGTTGAAATATA+GGG - chr4.4:4302742-4302761 MS.gene20195:intron 25.0%
! GTGTAGCAAATAAAAATAGT+AGG + chr4.4:4303437-4303456 None:intergenic 25.0%
! TAATTATAAAGGTAGCAAGT+TGG - chr4.4:4303194-4303213 MS.gene20195:CDS 25.0%
! TCAAAAGTAGATTGTAATGT+TGG - chr4.4:4302542-4302561 MS.gene20195:CDS 25.0%
! TTCAAAAGGACAATGAATTA+TGG - chr4.4:4304212-4304231 MS.gene20195:intron 25.0%
!! ATGTATTTTGTCCTATGTAA+TGG - chr4.4:4302472-4302491 MS.gene20195:CDS 25.0%
!! CACCTCATAAAAATATGTTT+TGG - chr4.4:4303964-4303983 MS.gene20195:CDS 25.0%
!! CTTTCTTCTTTAAGAATAAG+AGG + chr4.4:4303517-4303536 None:intergenic 25.0%
!! TGTATTTTGTCCTATGTAAT+GGG - chr4.4:4302473-4302492 MS.gene20195:CDS 25.0%
!! TTATGCACTTTTCCATATTT+TGG + chr4.4:4304063-4304082 None:intergenic 25.0%
!! TTGAATTTGAGATGTACAAA+GGG + chr4.4:4303117-4303136 None:intergenic 25.0%
!! TTTGAATTTGAGATGTACAA+AGG + chr4.4:4303118-4303137 None:intergenic 25.0%
!!! AAAATCTAATTTTGAAGACG+AGG + chr4.4:4302603-4302622 None:intergenic 25.0%
!!! CTTTACCCTTTAAAATTACA+AGG - chr4.4:4303916-4303935 MS.gene20195:CDS 25.0%
AAAGGACAATGAATTATGGA+AGG - chr4.4:4304216-4304235 MS.gene20195:intron 30.0%
AGCTACATGATTAGATAAAG+TGG + chr4.4:4304535-4304554 None:intergenic 30.0%
ATTATAAAGGTAGCAAGTTG+GGG - chr4.4:4303196-4303215 MS.gene20195:CDS 30.0%
ATTGAACCACTACTAATTCT+AGG + chr4.4:4304169-4304188 None:intergenic 30.0%
ATTTCTTCCATCTAATAGGT+GGG + chr4.4:4303676-4303695 None:intergenic 30.0%
CAGTATTTCTTCCATCTAAT+AGG + chr4.4:4303680-4303699 None:intergenic 30.0%
CTATTAGATGGAAGAAATAC+TGG - chr4.4:4303678-4303697 MS.gene20195:CDS 30.0%
GAATGAGATCATAAGTTCTA+GGG + chr4.4:4304468-4304487 None:intergenic 30.0%
GCAAATCAAACAGAGAAAAA+TGG + chr4.4:4304027-4304046 None:intergenic 30.0%
TACTGTTATACTATTGGAGA+TGG - chr4.4:4304725-4304744 MS.gene20195:CDS 30.0%
TATTTCTTCCATCTAATAGG+TGG + chr4.4:4303677-4303696 None:intergenic 30.0%
TATTTGGCATATATCAGGAT+AGG - chr4.4:4304500-4304519 MS.gene20195:CDS 30.0%
TCAAGCTGATTATAATGCTT+TGG + chr4.4:4302407-4302426 None:intergenic 30.0%
TCACTTGAAATAAGTACTTC+TGG - chr4.4:4302635-4302654 MS.gene20195:intron 30.0%
TGAATGAGATCATAAGTTCT+AGG + chr4.4:4304469-4304488 None:intergenic 30.0%
TGGTATGTATTTGATTGAAG+TGG - chr4.4:4303610-4303629 MS.gene20195:CDS 30.0%
TTGAAATAAGTACTTCTGGT+GGG - chr4.4:4302639-4302658 MS.gene20195:intron 30.0%
TTGAGAAAGCTGTTCAAAAT+TGG - chr4.4:4302900-4302919 MS.gene20195:CDS 30.0%
TTGTTGCGGAAAAAACTTAT+TGG - chr4.4:4304892-4304911 MS.gene20195:CDS 30.0%
! AAGAATCTTTTCTGGGTTAA+AGG + chr4.4:4304874-4304893 None:intergenic 30.0%
! AATAGACTCACATGGATTTT+AGG - chr4.4:4302437-4302456 MS.gene20195:CDS 30.0%
! ACACATTCAAGGTGATTTTT+AGG + chr4.4:4303824-4303843 None:intergenic 30.0%
! AGAATCTTTTCTGGGTTAAA+GGG + chr4.4:4304873-4304892 None:intergenic 30.0%
! ATCTTTGAAAGAGGTTTCAT+TGG - chr4.4:4304406-4304425 MS.gene20195:CDS 30.0%
! CTATTATGTCCCTTTTCAAA+AGG + chr4.4:4303228-4303247 None:intergenic 30.0%
! TACAAAAGCTCTTCTTTTGT+AGG + chr4.4:4302779-4302798 None:intergenic 30.0%
! TCTTTGAAAGAGGTTTCATT+GGG - chr4.4:4304407-4304426 MS.gene20195:CDS 30.0%
! TTATTTGCTACACAATTTGG+TGG - chr4.4:4303444-4303463 MS.gene20195:CDS 30.0%
!! AACATAATGGATCATGAAAG+TGG - chr4.4:4304846-4304865 MS.gene20195:CDS 30.0%
!! ATTTTTAGGCTTTTGCCAAA+TGG + chr4.4:4303810-4303829 None:intergenic 30.0%
!! CAAAATTGGATTCAAGTTGA+AGG - chr4.4:4302914-4302933 MS.gene20195:CDS 30.0%
!! GTTTAGTTTGTACACATTCA+AGG + chr4.4:4303835-4303854 None:intergenic 30.0%
!!! AAAGGTGTCTTATATTTTGG+AGG + chr4.4:4302818-4302837 None:intergenic 30.0%
AATTCAGCTCATGATACTCT+TGG - chr4.4:4304379-4304398 MS.gene20195:CDS 35.0%
ACAACATTCATAACCCAAAC+TGG + chr4.4:4304352-4304371 None:intergenic 35.0%
AGAAAGATGACCTTTCCATT+TGG - chr4.4:4303792-4303811 MS.gene20195:intron 35.0%
ATCAGAACTGCAATTGATAC+AGG - chr4.4:4303037-4303056 MS.gene20195:CDS 35.0%
CAAGCATAATCTCTACTTTG+AGG + chr4.4:4302848-4302867 None:intergenic 35.0%
CACTGCAGAGAAATTTCAAA+AGG - chr4.4:4304198-4304217 MS.gene20195:intron 35.0%
CGGAAAAAACTTATTGGACT+GGG - chr4.4:4304898-4304917 MS.gene20195:CDS 35.0%
CTATATCTTCCTTGAGCAAT+AGG + chr4.4:4304271-4304290 None:intergenic 35.0%
CTCAAAGTAGAGATTATGCT+TGG - chr4.4:4302846-4302865 MS.gene20195:CDS 35.0%
CTGATATTACCAGTAGTAAG+AGG + chr4.4:4303022-4303041 None:intergenic 35.0%
CTTGAAATAAGTACTTCTGG+TGG - chr4.4:4302638-4302657 MS.gene20195:intron 35.0%
GATATGAATGCTTATCTTGG+TGG - chr4.4:4304301-4304320 MS.gene20195:CDS 35.0%
GGAAAAAACTTATTGGACTG+GGG - chr4.4:4304899-4304918 MS.gene20195:CDS 35.0%
GTATCATGAGCTGAATTTGA+AGG + chr4.4:4304376-4304395 None:intergenic 35.0%
TAGATGGAAGAAATACTGGA+GGG - chr4.4:4303682-4303701 MS.gene20195:CDS 35.0%
TCACTATATGTCATGTCACA+TGG + chr4.4:4302680-4302699 None:intergenic 35.0%
TCAGAACTGCAATTGATACA+GGG - chr4.4:4303038-4303057 MS.gene20195:CDS 35.0%
TGGTGTATTTGGCATATATC+AGG - chr4.4:4304495-4304514 MS.gene20195:CDS 35.0%
TTAGATGGAAGAAATACTGG+AGG - chr4.4:4303681-4303700 MS.gene20195:CDS 35.0%
TTATCAGACTGGCAAATATG+TGG + chr4.4:4303872-4303891 None:intergenic 35.0%
TTTCTTCCAACATATGCGTT+TGG + chr4.4:4303765-4303784 None:intergenic 35.0%
! GAAAAAACTTATTGGACTGG+GGG - chr4.4:4304900-4304919 MS.gene20195:CDS 35.0%
! TATGTCCCTTTTCAAAAGGT+AGG + chr4.4:4303224-4303243 None:intergenic 35.0%
! TCAAATTTTCTACCTCTAGC+TGG + chr4.4:4302722-4302741 None:intergenic 35.0%
!! ACATGGATTTTAGGTGTAAG+TGG - chr4.4:4302446-4302465 MS.gene20195:CDS 35.0%
!! CTACTTTGAGGCCATTTAAA+AGG + chr4.4:4302836-4302855 None:intergenic 35.0%
AACACCTACTAACCAATCTG+AGG - chr4.4:4302514-4302533 MS.gene20195:CDS 40.0%
AAGAGATACACATGAAGCTC+AGG - chr4.4:4303264-4303283 MS.gene20195:intron 40.0%
AAGTTGCCAAACGCATATGT+TGG - chr4.4:4303756-4303775 MS.gene20195:intron 40.0%
AATACATACCATCTGTGCCA+TGG + chr4.4:4303601-4303620 None:intergenic 40.0%
AATACTTACCAAGCCATGTC+AGG + chr4.4:4303896-4303915 None:intergenic 40.0%
AATTTCTCTGCAGTGATGCT+TGG + chr4.4:4304193-4304212 None:intergenic 40.0%
AGAAAGCTTCACACCAATCA+TGG + chr4.4:4304438-4304457 None:intergenic 40.0%
AGATGGAAGAAATACTGGAG+GGG - chr4.4:4303683-4303702 MS.gene20195:CDS 40.0%
ATTCATGCAGCTGGTGTATT+TGG - chr4.4:4304484-4304503 MS.gene20195:CDS 40.0%
CATATCACTCCTATTGCTCA+AGG - chr4.4:4304259-4304278 MS.gene20195:CDS 40.0%
CATATTTGCTCCATGGAATG+AGG + chr4.4:4303396-4303415 None:intergenic 40.0%
CCAAGAGAAATAGACTCACA+TGG - chr4.4:4302429-4302448 MS.gene20195:CDS 40.0%
CCCAGAAAAGATTCTTGTTG+CGG - chr4.4:4304878-4304897 MS.gene20195:CDS 40.0%
CTATATGTCATGTCACATGG+TGG + chr4.4:4302677-4302696 None:intergenic 40.0%
CTCATTCCATGGAGCAAATA+TGG - chr4.4:4303394-4303413 MS.gene20195:intron 40.0%
CTGAATACAACTGTACCTTC+TGG + chr4.4:4304765-4304784 None:intergenic 40.0%
GAAAGAATCCAATAACCACC+TGG + chr4.4:4303650-4303669 None:intergenic 40.0%
GAACCACTACTAATTCTAGG+AGG + chr4.4:4304166-4304185 None:intergenic 40.0%
GAAGTGGAAGAGCAACATAA+TGG - chr4.4:4304833-4304852 MS.gene20195:CDS 40.0%
GAGCTTATTCCTCTTACTAC+TGG - chr4.4:4303010-4303029 MS.gene20195:CDS 40.0%
GCAAGCTTTGCAAATTTCCA+TGG - chr4.4:4303581-4303600 MS.gene20195:CDS 40.0%
GCGGAAAAAACTTATTGGAC+TGG - chr4.4:4304897-4304916 MS.gene20195:CDS 40.0%
GGTACAGTTGTATTCAGAGA+AGG - chr4.4:4304768-4304787 MS.gene20195:CDS 40.0%
GGTGATCCATTGTAATCCTT+GGG + chr4.4:4302386-4302405 None:intergenic 40.0%
GGTGGTTATTGGATTCTTTC+TGG - chr4.4:4303650-4303669 MS.gene20195:CDS 40.0%
GTACCTCCTAGAATTAGTAG+TGG - chr4.4:4304160-4304179 MS.gene20195:intron 40.0%
TACCAACCATATTTGCTCCA+TGG + chr4.4:4303403-4303422 None:intergenic 40.0%
TATGATCTCATTCATGCAGC+TGG - chr4.4:4304475-4304494 MS.gene20195:CDS 40.0%
TCAATGCTAAGCTCTTTGTG+TGG + chr4.4:4302884-4302903 None:intergenic 40.0%
TCATTGGGACTTACCATGAT+TGG - chr4.4:4304422-4304441 MS.gene20195:CDS 40.0%
TCCTGCTATGATTGGTATCA+TGG - chr4.4:4303312-4303331 MS.gene20195:intron 40.0%
TGAGCTTCATGTGTATCTCT+TGG + chr4.4:4303265-4303284 None:intergenic 40.0%
TGGAGATGGATAGAATCTTG+AGG - chr4.4:4304739-4304758 MS.gene20195:CDS 40.0%
TGGCATATATCAGGATAGGT+TGG - chr4.4:4304504-4304523 MS.gene20195:CDS 40.0%
TGGTGATCCATTGTAATCCT+TGG + chr4.4:4302387-4302406 None:intergenic 40.0%
TGTAATGTTGGATCAACAGC+AGG - chr4.4:4302554-4302573 MS.gene20195:CDS 40.0%
TTCCATGGAGCAAATATGGT+TGG - chr4.4:4303398-4303417 MS.gene20195:intron 40.0%
TTGAAGTGGATAGAGTCCTT+AGG - chr4.4:4303624-4303643 MS.gene20195:CDS 40.0%
TTGGTATCATGGCTTCACAA+AGG - chr4.4:4303323-4303342 MS.gene20195:intron 40.0%
TTTCCATGCACCCATTACAT+AGG + chr4.4:4302486-4302505 None:intergenic 40.0%
! CCATGTGAGTCTATTTCTCT+TGG + chr4.4:4302432-4302451 None:intergenic 40.0%
! CCGCAACAAGAATCTTTTCT+GGG + chr4.4:4304881-4304900 None:intergenic 40.0%
! CTTGGTGCAATCTTTGAAAG+AGG - chr4.4:4304397-4304416 MS.gene20195:CDS 40.0%
! GTGTTACTGATGCAATGAAG+TGG - chr4.4:4304817-4304836 MS.gene20195:CDS 40.0%
! TAGGCTTTTGCCAAATGGAA+AGG + chr4.4:4303805-4303824 None:intergenic 40.0%
! TATGCCTCAGATTGGTTAGT+AGG + chr4.4:4302521-4302540 None:intergenic 40.0%
! TCCGCAACAAGAATCTTTTC+TGG + chr4.4:4304882-4304901 None:intergenic 40.0%
!! AATTCTAGGAGGTACTGCAA+AGG + chr4.4:4304155-4304174 None:intergenic 40.0%
!! CTTTTGAGTATGCCTCAGAT+TGG + chr4.4:4302529-4302548 None:intergenic 40.0%
!! GGATTCAAGTTGAAGGTGAT+CGG - chr4.4:4302921-4302940 MS.gene20195:CDS 40.0%
!! GTCTTATATTTTGGAGGAGC+AGG + chr4.4:4302812-4302831 None:intergenic 40.0%
ACTGCAATTGATACAGGGTG+TGG - chr4.4:4303043-4303062 MS.gene20195:CDS 45.0%
AGAGGAGTTCCTGCTATGAT+TGG - chr4.4:4303304-4303323 MS.gene20195:intron 45.0%
AGCTCAGGTACAGTTTGCAT+TGG - chr4.4:4303279-4303298 MS.gene20195:intron 45.0%
CCAGTCTGATAATCCTGACA+TGG - chr4.4:4303880-4303899 MS.gene20195:intron 45.0%
CCATATCAAAAGCCCTTGCT+GGG + chr4.4:4303357-4303376 None:intergenic 45.0%
CCATGTCAGGATTATCAGAC+TGG + chr4.4:4303883-4303902 None:intergenic 45.0%
CGAGGACGATGATGAAATTG+AGG + chr4.4:4302585-4302604 None:intergenic 45.0%
CTGATAATCCTGACATGGCT+TGG - chr4.4:4303885-4303904 MS.gene20195:intron 45.0%
CTGGAGGGGAACTAGAAAAA+TGG - chr4.4:4304119-4304138 MS.gene20195:intron 45.0%
GAGCTGGTAACTTCTGGTAA+TGG + chr4.4:4304088-4304107 None:intergenic 45.0%
GATAGAATCTTGAGGCCAGA+AGG - chr4.4:4304747-4304766 MS.gene20195:CDS 45.0%
GCAAATTTCCATGGCACAGA+TGG - chr4.4:4303590-4303609 MS.gene20195:CDS 45.0%
GCAAGAGCAAGATTCCATAG+AGG - chr4.4:4303733-4303752 MS.gene20195:intron 45.0%
GCCATGATACCAATCATAGC+AGG + chr4.4:4303316-4303335 None:intergenic 45.0%
GTACAGTTTGCATTGGAGAG+AGG - chr4.4:4303286-4303305 MS.gene20195:intron 45.0%
TTGCTTGAGCTGGTAACTTC+TGG + chr4.4:4304094-4304113 None:intergenic 45.0%
! TTTCTGGCCCACCTATTAGA+TGG - chr4.4:4303666-4303685 MS.gene20195:CDS 45.0%
!! GGGTGCCTACCTTTTGAAAA+GGG - chr4.4:4303216-4303235 MS.gene20195:CDS 45.0%
!! TGTCCTATGTAATGGGTGCA+TGG - chr4.4:4302480-4302499 MS.gene20195:CDS 45.0%
AAAGGCTACCATACCCAGCA+AGG - chr4.4:4303341-4303360 MS.gene20195:intron 50.0%
AAGGCTACCATACCCAGCAA+GGG - chr4.4:4303342-4303361 MS.gene20195:intron 50.0%
ACCAGCTCAAGCAAAGTAGC+TGG - chr4.4:4304100-4304119 MS.gene20195:CDS 50.0%
AGCTCAAGCAAAGTAGCTGG+AGG - chr4.4:4304103-4304122 MS.gene20195:intron 50.0%
CCATGTCAAGATCCAGCTAG+AGG - chr4.4:4302707-4302726 MS.gene20195:intron 50.0%
CCTCTAGCTGGATCTTGACA+TGG + chr4.4:4302710-4302729 None:intergenic 50.0%
CTCAAGCAAAGTAGCTGGAG+GGG - chr4.4:4304105-4304124 MS.gene20195:intron 50.0%
GAAACATGGTACCTCCACCA+GGG + chr4.4:4302955-4302974 None:intergenic 50.0%
GAAGAAATACTGGAGGGGCT+GGG - chr4.4:4303688-4303707 MS.gene20195:CDS 50.0%
GCCATATCAAAAGCCCTTGC+TGG + chr4.4:4303358-4303377 None:intergenic 50.0%
GCGTTTGGCAACTTCCTCTA+TGG + chr4.4:4303750-4303769 None:intergenic 50.0%
GCTCAAGCAAAGTAGCTGGA+GGG - chr4.4:4304104-4304123 MS.gene20195:intron 50.0%
TCAAAAGCCCTTGCTGGGTA+TGG + chr4.4:4303352-4303371 None:intergenic 50.0%
TCCAATAACCACCTGGCCTA+AGG + chr4.4:4303643-4303662 None:intergenic 50.0%
TCCTTAGGCCAGGTGGTTAT+TGG - chr4.4:4303639-4303658 MS.gene20195:CDS 50.0%
! CCCAGCAAGGGCTTTTGATA+TGG - chr4.4:4303354-4303373 MS.gene20195:intron 50.0%
! TCCAGCTACTTTGCTTGAGC+TGG + chr4.4:4304104-4304123 None:intergenic 50.0%
!! GGGGTGCCTACCTTTTGAAA+AGG - chr4.4:4303215-4303234 MS.gene20195:CDS 50.0%
ATCAGCACCGCGAGGAAACA+TGG + chr4.4:4302969-4302988 None:intergenic 55.0%
GATAGAGTCCTTAGGCCAGG+TGG - chr4.4:4303632-4303651 MS.gene20195:CDS 55.0%
GGAAACATGGTACCTCCACC+AGG + chr4.4:4302956-4302975 None:intergenic 55.0%
GGAAGAAATACTGGAGGGGC+TGG - chr4.4:4303687-4303706 MS.gene20195:CDS 55.0%
GGGCTGGGAAAGAACTGAAG+AGG - chr4.4:4303703-4303722 MS.gene20195:intron 55.0%
GTGGATAGAGTCCTTAGGCC+AGG - chr4.4:4303629-4303648 MS.gene20195:CDS 55.0%
GTTCTCGCTGCCTCATTCCA+TGG - chr4.4:4303383-4303402 MS.gene20195:intron 55.0%
! GATCGGTTTCGATTCCCTGG+TGG - chr4.4:4302938-4302957 MS.gene20195:CDS 55.0%
! GGTACTGCAAAGGCACGTCT+TGG + chr4.4:4304145-4304164 None:intergenic 55.0%
! GGTGATCGGTTTCGATTCCC+TGG - chr4.4:4302935-4302954 MS.gene20195:CDS 55.0%
!! GGAGGTACCATGTTTCCTCG+CGG - chr4.4:4302959-4302978 MS.gene20195:CDS 55.0%
ATGTACGCATCAGCACCGCG+AGG + chr4.4:4302977-4302996 None:intergenic 60.0%
CGGTTTCGATTCCCTGGTGG+AGG - chr4.4:4302941-4302960 MS.gene20195:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 4302380 4304944 4302380 ID=MS.gene20195
chr4.4 mRNA 4302380 4304944 4302380 ID=MS.gene20195.t1;Parent=MS.gene20195
chr4.4 exon 4304258 4304944 4304258 ID=MS.gene20195.t1.exon1;Parent=MS.gene20195.t1
chr4.4 CDS 4304258 4304944 4304258 ID=cds.MS.gene20195.t1;Parent=MS.gene20195.t1
chr4.4 exon 4303909 4304119 4303909 ID=MS.gene20195.t1.exon2;Parent=MS.gene20195.t1
chr4.4 CDS 4303909 4304119 4303909 ID=cds.MS.gene20195.t1;Parent=MS.gene20195.t1
chr4.4 exon 4303418 4303715 4303418 ID=MS.gene20195.t1.exon3;Parent=MS.gene20195.t1
chr4.4 CDS 4303418 4303715 4303418 ID=cds.MS.gene20195.t1;Parent=MS.gene20195.t1
chr4.4 exon 4302803 4303267 4302803 ID=MS.gene20195.t1.exon4;Parent=MS.gene20195.t1
chr4.4 CDS 4302803 4303267 4302803 ID=cds.MS.gene20195.t1;Parent=MS.gene20195.t1
chr4.4 exon 4302380 4302608 4302380 ID=MS.gene20195.t1.exon5;Parent=MS.gene20195.t1
chr4.4 CDS 4302380 4302608 4302380 ID=cds.MS.gene20195.t1;Parent=MS.gene20195.t1
Gene Sequence

>MS.gene20195

ATGCCCAAGGATTACAATGGATCACCAAAGCATTATAATCAGCTTGAATCCAAGAGAAATAGACTCACATGGATTTTAGGTGTAAGTGGTCTATGTATTTTGTCCTATGTAATGGGTGCATGGAAAAATACAGCAACACCTACTAACCAATCTGAGGCATACTCAAAAGTAGATTGTAATGTTGGATCAACAGCAGGTTCTTCCTCAATTTCATCATCGTCCTCGTCTTCAAAATTAGATTTTGCTAGTCATCATTCACTTGAAATAAGTACTTCTGGTGGGATAAAAGAGTTTCCACCATGTGACATGACATATAGTGAATACACACCATGTCAAGATCCAGCTAGAGGTAGAAAATTTGACAGAAACATGTTGAAATATAGGGAGAGACATTGTCCTACAAAAGAAGAGCTTTTGTATTGTCTTATACCTGCTCCTCCAAAATATAAGACACCTTTTAAATGGCCTCAAAGTAGAGATTATGCTTGGTATGATAATATTCCACACAAAGAGCTTAGCATTGAGAAAGCTGTTCAAAATTGGATTCAAGTTGAAGGTGATCGGTTTCGATTCCCTGGTGGAGGTACCATGTTTCCTCGCGGTGCTGATGCGTACATTGATGACATTAATGAGCTTATTCCTCTTACTACTGGTAATATCAGAACTGCAATTGATACAGGGTGTGGTGTAAGTTCATTCATCTATACACTTAATCATGATTTGTTTTATGTTTTCCCTTTGTACATCTCAAATTCAAATCAAATGTTAGGGTTATGCATATGTTGCTAACACTTTATATACCTTCATGTGAATCTAATTATAAAGGTAGCAAGTTGGGGTGCCTACCTTTTGAAAAGGGACATAATAGCAATGTCATTTGCACCAAGAGATACACATGAAGCTCAGGTACAGTTTGCATTGGAGAGAGGAGTTCCTGCTATGATTGGTATCATGGCTTCACAAAGGCTACCATACCCAGCAAGGGCTTTTGATATGGCTCATTGTTCTCGCTGCCTCATTCCATGGAGCAAATATGGTTGGTATTCACACTTCTCCTACTATTTTTATTTGCTACACAATTTGGTGGTATTTTGCAATTAAGAGACAAATAGTCAATTAAGAGACATTTTAAATCCTCTTATTCTTAAAGAAGAAAGAATTAAAAAAATTCTTATGCCGACCATAAGTAATAAACTAATATGCAAGCTTTGCAAATTTCCATGGCACAGATGGTATGTATTTGATTGAAGTGGATAGAGTCCTTAGGCCAGGTGGTTATTGGATTCTTTCTGGCCCACCTATTAGATGGAAGAAATACTGGAGGGGCTGGGAAAGAACTGAAGAGGATTTAAAGCAAGAGCAAGATTCCATAGAGGAAGTTGCCAAACGCATATGTTGGAAGAAAGTTGTTGAGAAAGATGACCTTTCCATTTGGCAAAAGCCTAAAAATCACCTTGAATGTGTACAAACTAAACAAGTCTTTAAAACACCACATATTTGCCAGTCTGATAATCCTGACATGGCTTGGTAAGTATTCTTTACCCTTTAAAATTACAAGGTTAAATTCTCTTAGCAGTGAACTTACACCTCATAAAAATATGTTTTGGTACCTTTGCATGTTTAAAATTTGATATATTTTGTTTACCATTTTTCTCTGTTTGATTTGCAATTAAAAAGGTACCAAAATATGGAAAAGTGCATAACTCCATTACCAGAAGTTACCAGCTCAAGCAAAGTAGCTGGAGGGGAACTAGAAAAATGGCCAAGACGTGCCTTTGCAGTACCTCCTAGAATTAGTAGTGGTTCAATACCAAGCATCACTGCAGAGAAATTTCAAAAGGACAATGAATTATGGAAGGACAGAATGTCACATTACAAGCATATCACTCCTATTGCTCAAGGAAGATATAGAAACATTATTGATATGAATGCTTATCTTGGTGGATTTGCAGCAGCATTATTAAAATTTCCAGTTTGGGTTATGAATGTTGTTCCTTCAAATTCAGCTCATGATACTCTTGGTGCAATCTTTGAAAGAGGTTTCATTGGGACTTACCATGATTGGTGTGAAGCTTTCTCAACTTACCCTAGAACTTATGATCTCATTCATGCAGCTGGTGTATTTGGCATATATCAGGATAGGTTGGTATTTCCACTTTATCTAATCATGTAGCTTTAATAATTTGTTACATGATATTATAAGTTGTTGGTGAAGTGCAGTATAGTCTCTAAAATCAAAGAAATGATTCTTGAAATTGAAAATCATCTATCAGTTGGTATCATTTCTATATGTTTTATCATGTGTTTTTGACTGAATTAATTGTTATGTATGGAAGGTGCAACATTACTGTTATACTATTGGAGATGGATAGAATCTTGAGGCCAGAAGGTACAGTTGTATTCAGAGAAGGAGTAGAGCTTCTCACAAAGATTAAAAGTGTTACTGATGCAATGAAGTGGAAGAGCAACATAATGGATCATGAAAGTGGACCCTTTAACCCAGAAAAGATTCTTGTTGCGGAAAAAACTTATTGGACTGGGGGAGCTAAAGATAATAGCAACTAA
Protein sequence

>MS.gene20195.t1

MPKDYNGSPKHYNQLESKRNRLTWILGVSGLCILSYVMGAWKNTATPTNQSEAYSKVDCNVGSTAGSSSISSSSSSSKLDFASHHSLEISTSGGIKEFPPCDMTYSEYTPCQDPARGRKFDRNMLKYRERHCPTKEELLYCLIPAPPKYKTPFKWPQSRDYAWYDNIPHKELSIEKAVQNWIQVEGDRFRFPGGGTMFPRGADAYIDDINELIPLTTGNIRTAIDTGCGVASWGAYLLKRDIIAMSFAPRDTHEAQVQFALERGVPAMIGIMASQRLPYPARAFDMAHCSRCLIPWSKYDGMYLIEVDRVLRPGGYWILSGPPIRWKKYWRGWERTEEDLKQEQDSIEEVAKRICWKKVVEKDDLSIWQKPKNHLECVQTKQVFKTPHICQSDNPDMAWYQNMEKCITPLPEVTSSSKVAGGELEKWPRRAFAVPPRISSGSIPSITAEKFQKDNELWKDRMSHYKHITPIAQGRYRNIIDMNAYLGGFAAALLKFPVWVMNVVPSNSAHDTLGAIFERGFIGTYHDWCEAFSTYPRTYDLIHAAGVFGIYQDRCNITVILLEMDRILRPEGTVVFREGVELLTKIKSVTDAMKWKSNIMDHESGPFNPEKILVAEKTYWTGGAKDNSN