AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20350.t1 MTR_5g011050 96.625 800 27 0 1 800 55 854 0.0 1626
MS.gene20350.t1 MTR_5g011060 91.375 800 63 1 1 800 55 848 0.0 1538
MS.gene20350.t1 MTR_8g033080 68.773 807 242 4 1 800 246 1049 0.0 1181
MS.gene20350.t1 MTR_7g075910 68.618 803 245 4 1 800 316 1114 0.0 1174
MS.gene20350.t1 MTR_8g088690 54.156 794 342 7 20 799 56 841 0.0 873
MS.gene20350.t1 MTR_5g023050 53.015 796 329 10 31 793 81 864 0.0 867
MS.gene20350.t1 MTR_3g114840 53.934 788 339 9 31 799 68 850 0.0 865
MS.gene20350.t1 MTR_8g033080 67.221 601 188 3 1 595 246 843 0.0 861
MS.gene20350.t1 MTR_3g114840 54.274 702 297 9 31 713 68 764 0.0 760
MS.gene20350.t1 MTR_2g103730 46.667 795 377 16 20 789 25 797 0.0 717
MS.gene20350.t1 MTR_4g010650 47.204 769 369 14 36 789 50 796 0.0 712
MS.gene20350.t1 MTR_3g107360 46.134 776 374 16 36 790 59 811 0.0 674
MS.gene20350.t1 MTR_3g107320 44.819 772 387 16 36 790 66 815 0.0 657
MS.gene20350.t1 MTR_2g100250 41.406 768 377 21 36 789 36 744 0.0 539
MS.gene20350.t1 MTR_8g016160 39.517 787 372 25 36 799 39 744 2.20e-161 487
MS.gene20350.t1 MTR_1g083620 30.824 279 135 11 450 717 455 686 2.52e-21 100
MS.gene20350.t1 MTR_1g083620 28.685 251 117 7 201 428 130 341 3.02e-18 90.1
MS.gene20350.t1 MTR_1g083620 30.824 279 135 11 450 717 715 946 3.21e-21 100
MS.gene20350.t1 MTR_1g083620 28.685 251 117 7 201 428 390 601 2.52e-18 90.9
MS.gene20350.t1 MTR_2g435560 30.043 233 118 7 192 407 381 585 6.98e-20 95.9
MS.gene20350.t1 MTR_2g435560 33.166 199 100 8 492 684 784 955 1.21e-16 85.5
MS.gene20350.t1 MTR_2g435560 30.043 233 118 7 192 407 246 450 8.78e-20 95.5
MS.gene20350.t1 MTR_2g435560 33.166 199 100 8 492 684 649 820 8.61e-17 85.9
MS.gene20350.t1 MTR_5g023040 56.522 69 30 0 304 372 800 868 3.09e-18 90.1
MS.gene20350.t1 MTR_8g024030 62.712 59 15 1 455 513 1 52 2.42e-15 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20350.t1 AT2G42010 69.863 803 232 4 1 800 288 1083 0.0 1213
MS.gene20350.t1 AT2G42010 67.874 828 231 5 1 800 288 1108 0.0 1201
MS.gene20350.t1 AT4G11850 67.450 808 249 5 1 800 57 858 0.0 1162
MS.gene20350.t1 AT4G00240 66.625 803 258 4 1 800 132 927 0.0 1160
MS.gene20350.t1 AT4G00240 67.478 781 248 3 23 800 115 892 0.0 1150
MS.gene20350.t1 AT4G00240 67.696 777 245 3 27 800 2 775 0.0 1147
MS.gene20350.t1 AT4G11840 66.049 810 261 6 1 800 61 866 0.0 1134
MS.gene20350.t1 AT4G11830 66.292 801 256 6 9 800 61 856 0.0 1119
MS.gene20350.t1 AT4G11830 66.878 788 248 5 22 800 41 824 0.0 1118
MS.gene20350.t1 AT4G11840 64.851 808 244 6 1 800 61 836 0.0 1100
MS.gene20350.t1 AT4G35790 53.208 795 333 12 31 799 75 856 0.0 849
MS.gene20350.t1 AT4G35790 52.481 806 333 13 31 799 75 867 0.0 843
MS.gene20350.t1 AT4G11830 65.630 579 192 4 9 585 61 634 0.0 807
MS.gene20350.t1 AT4G11830 66.370 562 182 4 9 568 61 617 0.0 791
MS.gene20350.t1 AT3G15730 46.304 771 376 14 36 790 51 799 0.0 707
MS.gene20350.t1 AT1G52570 46.364 770 377 15 36 790 51 799 0.0 705
MS.gene20350.t1 AT1G52570 46.364 770 377 15 36 790 51 799 0.0 705
MS.gene20350.t1 AT1G52570 46.364 770 377 15 36 790 51 799 0.0 705
MS.gene20350.t1 AT5G25370 45.644 769 381 14 36 789 62 808 0.0 650
MS.gene20350.t1 AT5G25370 45.644 769 381 14 36 789 62 808 0.0 650
MS.gene20350.t1 AT5G25370 45.644 769 381 14 36 789 62 808 0.0 650
MS.gene20350.t1 AT4G35790 52.335 621 265 10 31 626 75 689 0.0 650
MS.gene20350.t1 AT5G25370 46.123 748 369 13 57 789 5 733 0.0 643
MS.gene20350.t1 AT1G55180 39.340 788 385 25 28 789 8 728 7.69e-168 503
MS.gene20350.t1 AT1G55180 39.511 777 380 24 36 789 41 750 3.01e-167 503
MS.gene20350.t1 AT3G16785 34.146 205 96 8 489 684 756 930 7.69e-19 92.4
MS.gene20350.t1 AT3G16785 30.085 236 115 7 208 427 390 591 9.70e-19 92.0
MS.gene20350.t1 AT3G16785 34.146 205 96 8 489 684 756 930 7.69e-19 92.4
MS.gene20350.t1 AT3G16785 30.085 236 115 7 208 427 390 591 9.70e-19 92.0
MS.gene20350.t1 AT3G16785 34.146 205 96 8 489 684 756 930 7.96e-19 92.4
MS.gene20350.t1 AT3G16785 30.085 236 115 7 208 427 390 591 1.22e-18 91.7
MS.gene20350.t1 AT3G16785 34.146 205 96 8 489 684 756 930 8.04e-19 92.4
MS.gene20350.t1 AT3G16785 30.085 236 115 7 208 427 390 591 1.17e-18 91.7
MS.gene20350.t1 AT3G16785 34.146 205 96 8 489 684 756 930 8.04e-19 92.4
MS.gene20350.t1 AT3G16785 30.085 236 115 7 208 427 390 591 1.17e-18 91.7
MS.gene20350.t1 AT3G16785 34.146 205 96 8 489 684 756 930 1.01e-18 92.0
MS.gene20350.t1 AT3G16785 30.085 236 115 7 208 427 390 591 1.11e-18 92.0
MS.gene20350.t1 AT3G05630 31.818 242 115 10 492 717 714 921 1.67e-17 88.2
MS.gene20350.t1 AT3G05630 28.634 227 104 6 204 407 381 572 1.39e-16 85.1

Find 157 sgRNAs with CRISPR-Local

Find 263 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAATTGTTTGTGTCTTAA+AGG 0.172001 5.1:+4655676 None:intergenic
GCCATGAGGTTTAGATTTCT+TGG 0.202085 5.1:+4652320 None:intergenic
ACCCTTTGTATTGCATCTTT+TGG 0.233281 5.1:+4653590 None:intergenic
TTCTTGGCTCGTCATATAAC+TGG 0.241998 5.1:-4653373 MS.gene20350:CDS
GATGGAAAAGATTGTACTTT+AGG 0.258837 5.1:-4655341 MS.gene20350:CDS
ACTAAACAGGGTTCTGTTAC+TGG 0.267976 5.1:-4653986 MS.gene20350:intron
TGGAAATCCTTTCACAGAAT+TGG 0.280674 5.1:+4653619 None:intergenic
TAGTGCCTTCCATTGATCTT+TGG 0.281191 5.1:+4652394 None:intergenic
TTTCCCATTCTCAATACTAA+TGG 0.285929 5.1:-4655704 MS.gene20350:CDS
CTTCCTAGTTTGAGGGTTGA+TGG 0.288084 5.1:-4655500 MS.gene20350:CDS
AATGGATGAAAATGTTTATA+TGG 0.293818 5.1:+4652008 None:intergenic
CACCAACACCATTACTATAA+AGG 0.306813 5.1:+4655615 None:intergenic
TAACCAACGCTCCTCAAAAT+TGG 0.309829 5.1:+4653895 None:intergenic
GGATGATGAATACGTGTTAA+TGG 0.315798 5.1:-4652433 MS.gene20350:CDS
CTACTCAGAGGATTCTCTTT+TGG 0.327286 5.1:-4652973 MS.gene20350:CDS
AGAAAGATTATAGCTTTCAT+TGG 0.342958 5.1:-4654607 MS.gene20350:CDS
CTCCGACACATTCTCTCTTT+AGG 0.344293 5.1:-4654552 MS.gene20350:CDS
AAAATTGTTTGTGTCTTAAA+GGG 0.345897 5.1:+4655677 None:intergenic
GGTCATTGAGTGAGTATAAC+TGG 0.346798 5.1:-4652197 MS.gene20350:CDS
CAAGGAGACTTGGCATGTTC+AGG 0.356759 5.1:-4653750 MS.gene20350:intron
GTGCTTCTTTGCCCAAGATC+TGG 0.359455 5.1:-4654994 MS.gene20350:CDS
CTCTGACATTATCGGCATTC+GGG 0.361714 5.1:-4653798 MS.gene20350:CDS
AACAATTTACAAGGCATTAG+AGG 0.367522 5.1:-4652740 MS.gene20350:CDS
ACTACAATGTTAGTCTCATT+AGG 0.367770 5.1:-4655373 MS.gene20350:CDS
CTTTCATAATCCTAACTTTG+TGG 0.375521 5.1:-4654503 MS.gene20350:intron
TTGAATGTGTGAGAAGAATA+AGG 0.385372 5.1:-4652218 MS.gene20350:CDS
TGATGTTGGTGGGAACATAA+AGG 0.391320 5.1:-4652061 MS.gene20350:CDS
CATATCAGCCTAATCATACT+TGG 0.394543 5.1:-4652347 MS.gene20350:CDS
GATTTGCATTCCAAAGTTGA+TGG 0.398771 5.1:-4653941 MS.gene20350:CDS
ATGATATATGTTCATTCAAA+AGG 0.399907 5.1:-4652465 MS.gene20350:CDS
GACAGAATCTCTGACATTAT+CGG 0.401822 5.1:-4653806 MS.gene20350:CDS
ACCAACACCATTACTATAAA+GGG 0.402130 5.1:+4655616 None:intergenic
GTACTTGAGGGTACACCTTC+AGG 0.405376 5.1:+4652998 None:intergenic
GTATAACTGGAGACAATATT+TGG 0.407854 5.1:-4652184 MS.gene20350:CDS
CTCACACATTCAATGCTCTC+TGG 0.408766 5.1:+4652228 None:intergenic
GGATAACTAACCACAAAGTT+AGG 0.408900 5.1:+4654493 None:intergenic
GAAACAATGAGAACCCTGTT+TGG 0.412454 5.1:-4655874 MS.gene20350:CDS
TTTATGTTCCCACCAACATC+AGG 0.412800 5.1:+4652063 None:intergenic
AAGGTTGTCTTCCTAGTTTG+AGG 0.414532 5.1:-4655508 MS.gene20350:CDS
ATTGTTGGTTCACAGATTAT+TGG 0.418383 5.1:-4655782 MS.gene20350:CDS
ATTGAACAGAACTTGGTTTG+TGG 0.418647 5.1:-4653488 MS.gene20350:intron
TGCCCAAGATCTGGAGGAAA+AGG 0.418793 5.1:-4654985 MS.gene20350:CDS
GTGAAGTGCTGATTCCAAAC+AGG 0.421259 5.1:+4655860 None:intergenic
CATTGATCTTTGGTTTATGT+TGG 0.423427 5.1:+4652404 None:intergenic
CTACAATGTTAGTCTCATTA+GGG 0.423726 5.1:-4655372 MS.gene20350:CDS
AGGTTGTCTTCCTAGTTTGA+GGG 0.429483 5.1:-4655507 MS.gene20350:CDS
TTGGTTGCCCAAGTATGATT+AGG 0.430796 5.1:+4652339 None:intergenic
TTTCAAGCACTCTTCAGTTA+GGG 0.433706 5.1:-4655016 MS.gene20350:CDS
TCAAATGTTGTAAGAGCTGC+TGG 0.434970 5.1:-4652628 MS.gene20350:CDS
TCTCTGACATTATCGGCATT+CGG 0.436671 5.1:-4653799 MS.gene20350:CDS
TTGCTTAATTGTCAGGAAGC+TGG 0.438888 5.1:-4654691 MS.gene20350:intron
AAGATTATAGCTTTCATTGG+AGG 0.443477 5.1:-4654604 MS.gene20350:CDS
TGTCATGATACAAAAGTTGA+AGG 0.446071 5.1:-4655731 MS.gene20350:CDS
ACATAGCTTGCTCAAACAAC+AGG 0.447421 5.1:-4654962 MS.gene20350:intron
AAAACAATGCAAATGATGTA+TGG 0.449561 5.1:-4652763 MS.gene20350:CDS
TCAGTATACACCTGTTGATA+AGG 0.452338 5.1:-4655642 MS.gene20350:CDS
TCACAGATTATTGGTGCAGT+TGG 0.452560 5.1:-4655773 MS.gene20350:CDS
GATGATGAATACGTGTTAAT+GGG 0.455690 5.1:-4652432 MS.gene20350:CDS
GGCATTGTAAAGCCTCTTGT+TGG 0.456829 5.1:-4652099 MS.gene20350:CDS
GATGTTGGTGGGAACATAAA+GGG 0.457449 5.1:-4652060 MS.gene20350:CDS
TCTGAAGCAAGATGTGCAAC+AGG 0.459931 5.1:+4655833 None:intergenic
TATGTAACTGTTTCTGTAGC+TGG 0.461426 5.1:-4655926 MS.gene20350:CDS
AAACTTGGTGGCAAAATTGA+AGG 0.468870 5.1:-4655980 MS.gene20350:CDS
GTAAAAGACAATGATATTGT+TGG 0.472603 5.1:-4655797 MS.gene20350:CDS
CCCACTTGAGGTTGATTCAA+AGG 0.475652 5.1:-4652121 MS.gene20350:CDS
ATTGATTCCAATTCTGTGAA+AGG 0.482984 5.1:-4653626 MS.gene20350:CDS
TTTCCATTAGTATTGAGAAT+GGG 0.483559 5.1:+4655701 None:intergenic
CTAAGTGGAAAATATGTAGC+AGG 0.483576 5.1:+4655575 None:intergenic
GGCAGCAGGACCATCAACTT+TGG 0.486946 5.1:+4653931 None:intergenic
TAAAGGGCTACCTTATCAAC+AGG 0.489987 5.1:+4655632 None:intergenic
GGAGGACTTGATTTATGTCT+CGG 0.491165 5.1:-4654586 MS.gene20350:CDS
CGTCGATTGGTCTATATCGT+TGG 0.492090 5.1:-4655407 MS.gene20350:CDS
CCTACTTCTAACAGCATGCT+TGG 0.497597 5.1:-4655257 MS.gene20350:CDS
CCACTTGAGGTTGATTCAAA+GGG 0.501387 5.1:-4652120 MS.gene20350:CDS
AAAATTGGTGAGGATGTCAT+AGG 0.508031 5.1:+4653910 None:intergenic
CCACCATCAACCCTCAAACT+AGG 0.511174 5.1:+4655497 None:intergenic
AATAAGTCAGGCGCGTCGAT+TGG 0.511402 5.1:-4655420 MS.gene20350:CDS
GTCATTGTGGATGCTGATGC+TGG 0.513953 5.1:-4654640 MS.gene20350:CDS
CTCTGAGTAGCTGTACTTGA+GGG 0.515931 5.1:+4652986 None:intergenic
TACAAGGCATTAGAGGAGGC+TGG 0.516518 5.1:-4652733 MS.gene20350:CDS
GCCCTTTATAGTAATGGTGT+TGG 0.518091 5.1:-4655617 MS.gene20350:CDS
CCAAGCATGCTGTTAGAAGT+AGG 0.522134 5.1:+4655257 None:intergenic
CTCATTGTTTCTAATTACAA+AGG 0.522279 5.1:+4655886 None:intergenic
ACCAAAAGATGCAATACAAA+GGG 0.524318 5.1:-4653591 MS.gene20350:intron
CTCCTATGTGTTCACTCCAT+AGG 0.524544 5.1:+4652274 None:intergenic
TCCTATGTGTTCACTCCATA+GGG 0.526136 5.1:+4652275 None:intergenic
AACAGTTACATACGGATCAC+TGG 0.528942 5.1:+4655937 None:intergenic
GATTGGTCTATATCGTTGGT+TGG 0.530259 5.1:-4655403 MS.gene20350:CDS
CCCTTTGAATCAACCTCAAG+TGG 0.531899 5.1:+4652120 None:intergenic
TATCTTCGATGCAATAAGTC+AGG 0.532907 5.1:-4655432 MS.gene20350:CDS
GAAATCTAAACCTCATGGCC+AGG 0.532948 5.1:-4652316 MS.gene20350:CDS
CAACATAAACCAAAGATCAA+TGG 0.533533 5.1:-4652403 MS.gene20350:CDS
TGCTGGACAAAACAATCCTC+AGG 0.533882 5.1:-4652611 MS.gene20350:intron
AAATCATGCCATGGTTGTCT+TGG 0.535876 5.1:+4653959 None:intergenic
AAACCTCATGGCCAGGTGCA+TGG 0.536424 5.1:-4652309 MS.gene20350:CDS
GTGAGGATGTCATAGGCAGC+AGG 0.549590 5.1:+4653917 None:intergenic
AAGGTAGCCCTTTATAGTAA+TGG 0.553403 5.1:-4655623 MS.gene20350:CDS
CTCATTAGGGATACTAGAGA+TGG 0.553430 5.1:-4655359 MS.gene20350:CDS
GAGAGTGCTTCTACTTGTGT+GGG 0.553897 5.1:-4655285 MS.gene20350:CDS
TGCAAACAACTTAATTCCGA+TGG 0.554309 5.1:-4653095 MS.gene20350:CDS
AGAGACACAGAAATTGCAAT+GGG 0.556833 5.1:-4652372 MS.gene20350:CDS
CCTCTGAGTAGCTGTACTTG+AGG 0.560599 5.1:+4652985 None:intergenic
CATGATGTGAGTTATGAGAA+TGG 0.562602 5.1:-4655470 MS.gene20350:CDS
CGGCGAGCTCGCGATTGCCA+AGG 0.562762 5.1:+4652665 None:intergenic
TCTTGGCTCGTCATATAACT+GGG 0.566567 5.1:-4653372 MS.gene20350:CDS
TCATTCAAAAGGAATGATAG+TGG 0.571139 5.1:-4652454 MS.gene20350:CDS
TGTGAAACATTCCCTGATGT+TGG 0.574572 5.1:-4652075 MS.gene20350:CDS
TGAAAATGAGTATGAACCAC+AGG 0.574704 5.1:-4652707 MS.gene20350:CDS
GTGATTCCTATGTGGCCTGA+AGG 0.577680 5.1:-4653013 MS.gene20350:CDS
TGAGAGTGCTTCTACTTGTG+TGG 0.580023 5.1:-4655286 MS.gene20350:CDS
CTTCTTTGCCCAAGATCTGG+AGG 0.588065 5.1:-4654991 MS.gene20350:CDS
TCTCCTTGTTATGCTCATCA+AGG 0.588490 5.1:+4653765 None:intergenic
GTTATGAGAATGGAAATTGT+TGG 0.588503 5.1:-4655460 MS.gene20350:CDS
AACCAAAAGATGCAATACAA+AGG 0.589106 5.1:-4653592 MS.gene20350:CDS
GGTACACCTTCAGGCCACAT+AGG 0.590249 5.1:+4653007 None:intergenic
CTTTATAGTAATGGTGTTGG+TGG 0.591047 5.1:-4655614 MS.gene20350:CDS
CAAGATGCTCATGTTCCACA+AGG 0.591161 5.1:-4655527 MS.gene20350:CDS
TTTACTGAAATACCCACTTG+AGG 0.593142 5.1:-4652133 MS.gene20350:CDS
ATGAGCATAACAAGGAGACT+TGG 0.594448 5.1:-4653760 MS.gene20350:CDS
ACCAAGAAATCTAAACCTCA+TGG 0.595362 5.1:-4652321 MS.gene20350:CDS
GAAACATTCCCTGATGTTGG+TGG 0.600474 5.1:-4652072 MS.gene20350:CDS
AATGTTTCACAGCCAACAAG+AGG 0.600850 5.1:+4652087 None:intergenic
GATGTATGGAACAATTTACA+AGG 0.602832 5.1:-4652749 MS.gene20350:CDS
TGTATATTGTGATTCCTATG+TGG 0.604015 5.1:-4653021 MS.gene20350:CDS
TCTTCTCAAAGCTAAATCAC+AGG 0.604747 5.1:-4655315 MS.gene20350:CDS
ATATCAGCCTAATCATACTT+GGG 0.606198 5.1:-4652346 MS.gene20350:CDS
GCTACAGAAACAGTTACATA+CGG 0.608100 5.1:+4655929 None:intergenic
GAGTGAACACATAGGAGGTG+TGG 0.608475 5.1:-4652268 MS.gene20350:CDS
TGGGATTCTTTCAAAGACCT+CGG 0.614331 5.1:-4653353 MS.gene20350:intron
CAGAATTGGAATCAATAGAA+CGG 0.617588 5.1:+4653633 None:intergenic
CTCAAAGCTAAATCACAGGA+AGG 0.618361 5.1:-4655311 MS.gene20350:CDS
CCTAGTTTGAGGGTTGATGG+TGG 0.621734 5.1:-4655497 MS.gene20350:CDS
ATGGTTATAGAATGTCCCTA+TGG 0.625370 5.1:-4652290 MS.gene20350:CDS
TTGTTATGCTCATCAAGGCA+AGG 0.628139 5.1:+4653770 None:intergenic
AATTTACAAGGCATTAGAGG+AGG 0.630851 5.1:-4652737 MS.gene20350:CDS
TTGGAATGCAAATCATGCCA+TGG 0.632340 5.1:+4653950 None:intergenic
TTATGTTCCCACCAACATCA+GGG 0.635043 5.1:+4652064 None:intergenic
ACATTCTATAACCATGCACC+TGG 0.636369 5.1:+4652298 None:intergenic
AACGCTCCTCAAAATTGGTG+AGG 0.637134 5.1:+4653900 None:intergenic
TAACCATGCACCTGGCCATG+AGG 0.638841 5.1:+4652306 None:intergenic
AAGAAATTCAAGTAGTCCTG+TGG 0.640692 5.1:+4652691 None:intergenic
TAGAGACACAGAAATTGCAA+TGG 0.640880 5.1:-4652373 MS.gene20350:CDS
TCATCAGAAGACAGTCATTG+TGG 0.641162 5.1:-4654653 MS.gene20350:CDS
GTCCTAAAGAGAGAATGTGT+CGG 0.643759 5.1:+4654550 None:intergenic
ATAAACCAAAGATCAATGGA+AGG 0.645459 5.1:-4652399 MS.gene20350:CDS
TGAAGTGCTGATTCCAAACA+GGG 0.647064 5.1:+4655861 None:intergenic
ATGAAAGTCATCTTTGTGTG+TGG 0.650414 5.1:+4654519 None:intergenic
TTGCCTTGATGAGCATAACA+AGG 0.672972 5.1:-4653768 MS.gene20350:CDS
CTGGTTGTCCAAGACAACCA+TGG 0.675621 5.1:-4653967 MS.gene20350:CDS
CTATGGAGTGAACACATAGG+AGG 0.681727 5.1:-4652273 MS.gene20350:CDS
CCTTTGAATCAACCTCAAGT+GGG 0.682667 5.1:+4652121 None:intergenic
AAACATTCCCTGATGTTGGT+GGG 0.683000 5.1:-4652071 MS.gene20350:CDS
ACTTATGAACACTTATGACG+AGG 0.684284 5.1:-4655052 MS.gene20350:CDS
GAGACTAACATTGTAGTACA+CGG 0.710080 5.1:+4655379 None:intergenic
CCTCAAGTACAGCTACTCAG+AGG 0.711303 5.1:-4652985 MS.gene20350:CDS
TCCCTATGGAGTGAACACAT+AGG 0.736923 5.1:-4652276 MS.gene20350:CDS
AAACTAGGAAGACAACCTTG+TGG 0.743773 5.1:+4655512 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACTAATGAAAATAAAAAA+TGG + chr5.1:4653585-4653604 None:intergenic 10.0%
!! AAAAAATCTAAATTTGAGAA+TGG + chr5.1:4654504-4654523 None:intergenic 15.0%
!! TATCAGAAACATAAATAAAA+AGG + chr5.1:4652852-4652871 None:intergenic 15.0%
!!! ATTTGAAATGTTTTCATTAT+CGG + chr5.1:4655412-4655431 None:intergenic 15.0%
!!! CATAAAAATTTCTATTTTGA+TGG - chr5.1:4655149-4655168 MS.gene20350:intron 15.0%
!!! TTATTTATGTTTCTGATATT+TGG - chr5.1:4652854-4652873 MS.gene20350:intron 15.0%
!!! TTTAGATTTTTTGTTATGTT+AGG - chr5.1:4654512-4654531 MS.gene20350:CDS 15.0%
!! AAAATTGTTTGTGTCTTAAA+GGG + chr5.1:4652380-4652399 None:intergenic 20.0%
!! AAATCAACATTTAAGAGTTT+AGG + chr5.1:4655263-4655282 None:intergenic 20.0%
!! AAATTGAAGAAGTTTCTTTA+AGG + chr5.1:4654357-4654376 None:intergenic 20.0%
!! AATTGAAGAAGTTTCTTTAA+GGG + chr5.1:4654356-4654375 None:intergenic 20.0%
!! ATAAAACTAACTTGATCAAA+AGG + chr5.1:4655511-4655530 None:intergenic 20.0%
!! ATCAATAAATCATGGTAATT+CGG + chr5.1:4652971-4652990 None:intergenic 20.0%
!! ATGATATATGTTCATTCAAA+AGG - chr5.1:4655589-4655608 MS.gene20350:CDS 20.0%
!! CAGATAATAGAAATAGTTAA+AGG - chr5.1:4653307-4653326 MS.gene20350:intron 20.0%
!!! ATTGAGAATCAATATTTTCT+TGG - chr5.1:4654665-4654684 MS.gene20350:CDS 20.0%
!!! TAAACTCTTAAATGTTGATT+TGG - chr5.1:4655262-4655281 MS.gene20350:CDS 20.0%
!!! TCATTATTGTTTGCATATTT+TGG - chr5.1:4655530-4655549 MS.gene20350:CDS 20.0%
!!! TGTTTTATTTGTCATAATAG+AGG - chr5.1:4654016-4654035 MS.gene20350:intron 20.0%
!!! TTTAATTTGCATACTAAACA+GGG - chr5.1:4654056-4654075 MS.gene20350:intron 20.0%
!!! TTTTAATTTGCATACTAAAC+AGG - chr5.1:4654055-4654074 MS.gene20350:intron 20.0%
! AAAACAATGCAAATGATGTA+TGG - chr5.1:4655291-4655310 MS.gene20350:CDS 25.0%
! AAAATGTGTTTACACATGAT+GGG - chr5.1:4653650-4653669 MS.gene20350:intron 25.0%
! ACCATGATTTATTGATATGT+AGG - chr5.1:4652975-4652994 MS.gene20350:CDS 25.0%
! ACCTACATATCAATAAATCA+TGG + chr5.1:4652979-4652998 None:intergenic 25.0%
! AGAAAGATTATAGCTTTCAT+TGG - chr5.1:4653447-4653466 MS.gene20350:CDS 25.0%
! ATAAAACAATTACAACGCAA+AGG + chr5.1:4654004-4654023 None:intergenic 25.0%
! CAAAATTGTTTGTGTCTTAA+AGG + chr5.1:4652381-4652400 None:intergenic 25.0%
! GAAAAGGAATGAATATAACA+AGG + chr5.1:4654926-4654945 None:intergenic 25.0%
! GTAAAAGACAATGATATTGT+TGG - chr5.1:4652257-4652276 MS.gene20350:CDS 25.0%
! TAAAATGTGTTTACACATGA+TGG - chr5.1:4653649-4653668 MS.gene20350:intron 25.0%
! TATAATATATAGGTTGCAAC+AGG - chr5.1:4654897-4654916 MS.gene20350:intron 25.0%
! TGATTTATTGATATGTAGGT+TGG - chr5.1:4652979-4652998 MS.gene20350:CDS 25.0%
!! ACATATTTTCCACTTAGAAA+AGG - chr5.1:4652485-4652504 MS.gene20350:CDS 25.0%
!! ATTCCTTTTCTTTTATTCAC+AGG - chr5.1:4654936-4654955 MS.gene20350:intron 25.0%
!! TTTCCATTAGTATTGAGAAT+GGG + chr5.1:4652356-4652375 None:intergenic 25.0%
!!! AAGGTGCAATCTTTTTAAAA+AGG - chr5.1:4653326-4653345 MS.gene20350:intron 25.0%
!!! ACTGCTTTTTCTTTTTCAAA+AGG - chr5.1:4653909-4653928 MS.gene20350:CDS 25.0%
!!! CTCATTGTTTCTAATTACAA+AGG + chr5.1:4652171-4652190 None:intergenic 25.0%
!!! TCATGACATAATTTTTCAAC+TGG + chr5.1:4652311-4652330 None:intergenic 25.0%
!!! TTCTTTCTGTTTGATTTTGT+TGG + chr5.1:4655003-4655022 None:intergenic 25.0%
!!! TTTTCATTAGTTTTCATTGC+CGG - chr5.1:4653592-4653611 MS.gene20350:CDS 25.0%
!!! TTTTCCATTAGTATTGAGAA+TGG + chr5.1:4652357-4652376 None:intergenic 25.0%
AACCAAAAGATGCAATACAA+AGG - chr5.1:4654462-4654481 MS.gene20350:intron 30.0%
AAGATTATAGCTTTCATTGG+AGG - chr5.1:4653450-4653469 MS.gene20350:CDS 30.0%
ACATACGTGTCTTAGAATTT+CGG + chr5.1:4653852-4653871 None:intergenic 30.0%
ACCAAAAGATGCAATACAAA+GGG - chr5.1:4654463-4654482 MS.gene20350:intron 30.0%
ACCAACACCATTACTATAAA+GGG + chr5.1:4652441-4652460 None:intergenic 30.0%
ACGGAAGATCTTCAATTTAA+AGG + chr5.1:4654405-4654424 None:intergenic 30.0%
AGAGTCTGTAGAATTGAATT+AGG - chr5.1:4653821-4653840 MS.gene20350:CDS 30.0%
ATAAACCAAAGATCAATGGA+AGG - chr5.1:4655655-4655674 MS.gene20350:CDS 30.0%
ATATCAGCCTAATCATACTT+GGG - chr5.1:4655708-4655727 MS.gene20350:CDS 30.0%
CAACATAAACCAAAGATCAA+TGG - chr5.1:4655651-4655670 MS.gene20350:CDS 30.0%
CAGAATTGGAATCAATAGAA+CGG + chr5.1:4654424-4654443 None:intergenic 30.0%
CTAGCTAAAGCTTATAAAAC+CGG + chr5.1:4653694-4653713 None:intergenic 30.0%
CTTTCATAATCCTAACTTTG+TGG - chr5.1:4653551-4653570 MS.gene20350:intron 30.0%
GAAAAAGAAAAAGCAGTCTA+TGG + chr5.1:4653906-4653925 None:intergenic 30.0%
GAGTAAAAACAAAATACCTG+AGG + chr5.1:4655462-4655481 None:intergenic 30.0%
GATGATGAATACGTGTTAAT+GGG - chr5.1:4655622-4655641 MS.gene20350:CDS 30.0%
GATGGAAAAGATTGTACTTT+AGG - chr5.1:4652713-4652732 MS.gene20350:CDS 30.0%
GATGTATGGAACAATTTACA+AGG - chr5.1:4655305-4655324 MS.gene20350:CDS 30.0%
GCAAAGGACAACATATAAAA+TGG + chr5.1:4653988-4654007 None:intergenic 30.0%
GTATAACTGGAGACAATATT+TGG - chr5.1:4655870-4655889 MS.gene20350:CDS 30.0%
GTCAAATATCTCCATTATTG+TGG + chr5.1:4654771-4654790 None:intergenic 30.0%
GTTATGAGAATGGAAATTGT+TGG - chr5.1:4652594-4652613 MS.gene20350:intron 30.0%
TATGGAGATTAAAAATGCGA+AGG + chr5.1:4653888-4653907 None:intergenic 30.0%
TCAGTCTTACCTAAAAAACT+TGG - chr5.1:4652059-4652078 MS.gene20350:CDS 30.0%
TCATTCAAAAGGAATGATAG+TGG - chr5.1:4655600-4655619 MS.gene20350:CDS 30.0%
TGTATATTGTGATTCCTATG+TGG - chr5.1:4655033-4655052 MS.gene20350:CDS 30.0%
TGTCATGATACAAAAGTTGA+AGG - chr5.1:4652323-4652342 MS.gene20350:CDS 30.0%
TTACTTCATTGAACAGAACT+TGG - chr5.1:4654559-4654578 MS.gene20350:CDS 30.0%
TTGAATGTGTGAGAAGAATA+AGG - chr5.1:4655836-4655855 MS.gene20350:CDS 30.0%
TTTCCCATTCTCAATACTAA+TGG - chr5.1:4652350-4652369 MS.gene20350:CDS 30.0%
! AACAATTTACAAGGCATTAG+AGG - chr5.1:4655314-4655333 MS.gene20350:CDS 30.0%
! ACTACAATGTTAGTCTCATT+AGG - chr5.1:4652681-4652700 MS.gene20350:CDS 30.0%
! ATTGATTCCAATTCTGTGAA+AGG - chr5.1:4654428-4654447 MS.gene20350:intron 30.0%
! ATTTTGCCACCAAGTTTTTT+AGG + chr5.1:4652071-4652090 None:intergenic 30.0%
! CATTGATCTTTGGTTTATGT+TGG + chr5.1:4655653-4655672 None:intergenic 30.0%
! CTACAATGTTAGTCTCATTA+GGG - chr5.1:4652682-4652701 MS.gene20350:CDS 30.0%
! TAACATGACACCATTTTAAG+TGG + chr5.1:4655124-4655143 None:intergenic 30.0%
! TAATGCCATACTTTTAACAC+CGG + chr5.1:4653614-4653633 None:intergenic 30.0%
! TACTTGAATTTCTTTTGCCT+TGG - chr5.1:4655372-4655391 MS.gene20350:CDS 30.0%
! TATTTTCCACTTAGAAAAGG+TGG - chr5.1:4652488-4652507 MS.gene20350:CDS 30.0%
!! ATTGTTGGTTCACAGATTAT+TGG - chr5.1:4652272-4652291 MS.gene20350:CDS 30.0%
!! CAGATGGAGTATCATTTTAT+TGG + chr5.1:4654746-4654765 None:intergenic 30.0%
!!! ATTGCATCTTTTGGTTCTTT+TGG + chr5.1:4654458-4654477 None:intergenic 30.0%
!!! ATTTTTAGCGCAATTTCCAT+CGG + chr5.1:4654978-4654997 None:intergenic 30.0%
!!! TTGCATATTTTGGAACCTAT+AGG - chr5.1:4655540-4655559 MS.gene20350:CDS 30.0%
!!! TTGCTTTTTGCTTAATTGTC+AGG - chr5.1:4653356-4653375 MS.gene20350:CDS 30.0%
AAAATTGGTGAGGATGTCAT+AGG + chr5.1:4654147-4654166 None:intergenic 35.0%
AAGAAATTCAAGTAGTCCTG+TGG + chr5.1:4655366-4655385 None:intergenic 35.0%
AAGGTAGCCCTTTATAGTAA+TGG - chr5.1:4652431-4652450 MS.gene20350:CDS 35.0%
ACCAAGAAATCTAAACCTCA+TGG - chr5.1:4655733-4655752 MS.gene20350:CDS 35.0%
ACTTATGAACACTTATGACG+AGG - chr5.1:4653002-4653021 MS.gene20350:CDS 35.0%
AGAGACACAGAAATTGCAAT+GGG - chr5.1:4655682-4655701 MS.gene20350:CDS 35.0%
ATGAAAGTCATCTTTGTGTG+TGG + chr5.1:4653538-4653557 None:intergenic 35.0%
ATGGTTATAGAATGTCCCTA+TGG - chr5.1:4655764-4655783 MS.gene20350:CDS 35.0%
ATTCTACAGACTCTTAACTC+AGG + chr5.1:4653815-4653834 None:intergenic 35.0%
ATTGCCTATTCAAAAGTAGC+AGG + chr5.1:4652918-4652937 None:intergenic 35.0%
CACCAACACCATTACTATAA+AGG + chr5.1:4652442-4652461 None:intergenic 35.0%
CATATCAGCCTAATCATACT+TGG - chr5.1:4655707-4655726 MS.gene20350:CDS 35.0%
CATGATGTGAGTTATGAGAA+TGG - chr5.1:4652584-4652603 MS.gene20350:intron 35.0%
CTAAGTGGAAAATATGTAGC+AGG + chr5.1:4652482-4652501 None:intergenic 35.0%
GACAGAATCTCTGACATTAT+CGG - chr5.1:4654248-4654267 MS.gene20350:intron 35.0%
GAGACTAACATTGTAGTACA+CGG + chr5.1:4652678-4652697 None:intergenic 35.0%
GATTTGCATTCCAAAGTTGA+TGG - chr5.1:4654113-4654132 MS.gene20350:intron 35.0%
GCACCTGTGAATAAAAGAAA+AGG + chr5.1:4654942-4654961 None:intergenic 35.0%
GCTACAGAAACAGTTACATA+CGG + chr5.1:4652128-4652147 None:intergenic 35.0%
GCTTATAAAACCGGATTGTA+TGG + chr5.1:4653685-4653704 None:intergenic 35.0%
GCTTCCTTTACCACTTAAAA+TGG - chr5.1:4655111-4655130 MS.gene20350:intron 35.0%
GGATAACTAACCACAAAGTT+AGG + chr5.1:4653564-4653583 None:intergenic 35.0%
GGATGATGAATACGTGTTAA+TGG - chr5.1:4655621-4655640 MS.gene20350:CDS 35.0%
GTCTTACCTAAAAAACTTGG+TGG - chr5.1:4652062-4652081 MS.gene20350:CDS 35.0%
TAGAGACACAGAAATTGCAA+TGG - chr5.1:4655681-4655700 MS.gene20350:CDS 35.0%
TATCTTCGATGCAATAAGTC+AGG - chr5.1:4652622-4652641 MS.gene20350:CDS 35.0%
TCAACCATTAAACTCTGTGA+CGG + chr5.1:4653936-4653955 None:intergenic 35.0%
TCAGTATACACCTGTTGATA+AGG - chr5.1:4652412-4652431 MS.gene20350:CDS 35.0%
TCTTCTCAAAGCTAAATCAC+AGG - chr5.1:4652739-4652758 MS.gene20350:CDS 35.0%
TGAAAATGAGTATGAACCAC+AGG - chr5.1:4655347-4655366 MS.gene20350:CDS 35.0%
TGCAAACAACTTAATTCCGA+TGG - chr5.1:4654959-4654978 MS.gene20350:intron 35.0%
TGGAAATCCTTTCACAGAAT+TGG + chr5.1:4654438-4654457 None:intergenic 35.0%
TTTACTGAAATACCCACTTG+AGG - chr5.1:4655921-4655940 MS.gene20350:CDS 35.0%
TTTCAAGCACTCTTCAGTTA+GGG - chr5.1:4653038-4653057 MS.gene20350:CDS 35.0%
! AAACTTGGTGGCAAAATTGA+AGG - chr5.1:4652074-4652093 MS.gene20350:CDS 35.0%
! AATTTACAAGGCATTAGAGG+AGG - chr5.1:4655317-4655336 MS.gene20350:CDS 35.0%
! ACACTTTTCACAAGAAAGTG+GGG - chr5.1:4652027-4652046 MS.gene20350:CDS 35.0%
! ACCCTTTGTATTGCATCTTT+TGG + chr5.1:4654467-4654486 None:intergenic 35.0%
! ACTCTATTAGTCTATTCCCT+TGG - chr5.1:4652884-4652903 MS.gene20350:intron 35.0%
! ACTTTTCCACCTTTTCTAAG+TGG + chr5.1:4652497-4652516 None:intergenic 35.0%
! ATTGAACAGAACTTGGTTTG+TGG - chr5.1:4654566-4654585 MS.gene20350:CDS 35.0%
! CTTTATAGTAATGGTGTTGG+TGG - chr5.1:4652440-4652459 MS.gene20350:CDS 35.0%
! GACACTTTTCACAAGAAAGT+GGG - chr5.1:4652026-4652045 MS.gene20350:CDS 35.0%
!! CGGTTTTATAAGCTTTAGCT+AGG - chr5.1:4653692-4653711 MS.gene20350:intron 35.0%
!! GACACCATTTTAAGTGGTAA+AGG + chr5.1:4655118-4655137 None:intergenic 35.0%
!! GCTTGCAATTCTGTTATTTC+TGG - chr5.1:4653174-4653193 MS.gene20350:intron 35.0%
!! GCTTTTGAGTAAGTACCTAT+AGG + chr5.1:4655558-4655577 None:intergenic 35.0%
!! TATGTAACTGTTTCTGTAGC+TGG - chr5.1:4652128-4652147 MS.gene20350:CDS 35.0%
!! TTTTCAAGCACTCTTCAGTT+AGG - chr5.1:4653037-4653056 MS.gene20350:CDS 35.0%
AAACATTCCCTGATGTTGGT+GGG - chr5.1:4655983-4656002 MS.gene20350:CDS 40.0%
AAACTAGGAAGACAACCTTG+TGG + chr5.1:4652545-4652564 None:intergenic 40.0%
AAATCATGCCATGGTTGTCT+TGG + chr5.1:4654098-4654117 None:intergenic 40.0%
AACAGTTACATACGGATCAC+TGG + chr5.1:4652120-4652139 None:intergenic 40.0%
AAGGTTGTCTTCCTAGTTTG+AGG - chr5.1:4652546-4652565 MS.gene20350:intron 40.0%
AATGTTTCACAGCCAACAAG+AGG + chr5.1:4655970-4655989 None:intergenic 40.0%
ACATTCTATAACCATGCACC+TGG + chr5.1:4655759-4655778 None:intergenic 40.0%
ACTAAACAGGGTTCTGTTAC+TGG - chr5.1:4654068-4654087 MS.gene20350:intron 40.0%
AGGTTGTCTTCCTAGTTTGA+GGG - chr5.1:4652547-4652566 MS.gene20350:intron 40.0%
ATGAGCATAACAAGGAGACT+TGG - chr5.1:4654294-4654313 MS.gene20350:intron 40.0%
CAACCATTAAACTCTGTGAC+GGG + chr5.1:4653935-4653954 None:intergenic 40.0%
CCTATTCAAAAGTAGCAGGA+AGG + chr5.1:4652914-4652933 None:intergenic 40.0%
CCTTTGAATCAACCTCAAGT+GGG + chr5.1:4655936-4655955 None:intergenic 40.0%
CGGTAATAGAAGTAAGCAAC+TGG + chr5.1:4652951-4652970 None:intergenic 40.0%
CTCAAAGCTAAATCACAGGA+AGG - chr5.1:4652743-4652762 MS.gene20350:CDS 40.0%
CTCATTAGGGATACTAGAGA+TGG - chr5.1:4652695-4652714 MS.gene20350:CDS 40.0%
CTCCATCTGTTCCACAATAA+TGG - chr5.1:4654757-4654776 MS.gene20350:intron 40.0%
CTCCATTATTGTGGAACAGA+TGG + chr5.1:4654762-4654781 None:intergenic 40.0%
GAAACAATGAGAACCCTGTT+TGG - chr5.1:4652180-4652199 MS.gene20350:CDS 40.0%
GCCATGAGGTTTAGATTTCT+TGG + chr5.1:4655737-4655756 None:intergenic 40.0%
GCCCTTTATAGTAATGGTGT+TGG - chr5.1:4652437-4652456 MS.gene20350:CDS 40.0%
GGAGGACTTGATTTATGTCT+CGG - chr5.1:4653468-4653487 MS.gene20350:CDS 40.0%
GGTCATTGAGTGAGTATAAC+TGG - chr5.1:4655857-4655876 MS.gene20350:CDS 40.0%
GTATGGCATTAAGTGAGACA+TGG - chr5.1:4653623-4653642 MS.gene20350:CDS 40.0%
GTCCTAAAGAGAGAATGTGT+CGG + chr5.1:4653507-4653526 None:intergenic 40.0%
TAAAACCGGATTGTATGGTG+AGG + chr5.1:4653680-4653699 None:intergenic 40.0%
TAAAGGGCTACCTTATCAAC+AGG + chr5.1:4652425-4652444 None:intergenic 40.0%
TAACCAACGCTCCTCAAAAT+TGG + chr5.1:4654162-4654181 None:intergenic 40.0%
TAGTGCCTTCCATTGATCTT+TGG + chr5.1:4655663-4655682 None:intergenic 40.0%
TCAAATGTTGTAAGAGCTGC+TGG - chr5.1:4655426-4655445 MS.gene20350:CDS 40.0%
TCACAGATTATTGGTGCAGT+TGG - chr5.1:4652281-4652300 MS.gene20350:CDS 40.0%
TCATCAGAAGACAGTCATTG+TGG - chr5.1:4653401-4653420 MS.gene20350:CDS 40.0%
TCCTATGTGTTCACTCCATA+GGG + chr5.1:4655782-4655801 None:intergenic 40.0%
TCTCTGACATTATCGGCATT+CGG - chr5.1:4654255-4654274 MS.gene20350:intron 40.0%
TGACCTATCAGATCTTTGGT+AGG + chr5.1:4653736-4653755 None:intergenic 40.0%
TGGGATTCTTTCAAAGACCT+CGG - chr5.1:4654701-4654720 MS.gene20350:intron 40.0%
TGTGAAACATTCCCTGATGT+TGG - chr5.1:4655979-4655998 MS.gene20350:CDS 40.0%
TTAGAACTCAATACTCACCG+AGG + chr5.1:4654721-4654740 None:intergenic 40.0%
TTATGTTCCCACCAACATCA+GGG + chr5.1:4655993-4656012 None:intergenic 40.0%
TTGCCTTGATGAGCATAACA+AGG - chr5.1:4654286-4654305 MS.gene20350:intron 40.0%
TTGCTTAATTGTCAGGAAGC+TGG - chr5.1:4653363-4653382 MS.gene20350:CDS 40.0%
TTGGAATGCAAATCATGCCA+TGG + chr5.1:4654107-4654126 None:intergenic 40.0%
TTGGTTGCCCAAGTATGATT+AGG + chr5.1:4655718-4655737 None:intergenic 40.0%
TTGTTATGCTCATCAAGGCA+AGG + chr5.1:4654287-4654306 None:intergenic 40.0%
TTTATGTTCCCACCAACATC+AGG + chr5.1:4655994-4656013 None:intergenic 40.0%
! CACTTTTCACAAGAAAGTGG+GGG - chr5.1:4652028-4652047 MS.gene20350:CDS 40.0%
! CATTGCCGGTGTTAAAAGTA+TGG - chr5.1:4653606-4653625 MS.gene20350:CDS 40.0%
! CCACTTGAGGTTGATTCAAA+GGG - chr5.1:4655934-4655953 MS.gene20350:CDS 40.0%
! CTACTCAGAGGATTCTCTTT+TGG - chr5.1:4655081-4655100 MS.gene20350:intron 40.0%
! GATGTTGGTGGGAACATAAA+GGG - chr5.1:4655994-4656013 MS.gene20350:CDS 40.0%
! GGACACTTTTCACAAGAAAG+TGG - chr5.1:4652025-4652044 MS.gene20350:CDS 40.0%
! TCTCCTTGTTATGCTCATCA+AGG + chr5.1:4654292-4654311 None:intergenic 40.0%
! TCTTGGCTCGTCATATAACT+GGG - chr5.1:4654682-4654701 MS.gene20350:intron 40.0%
! TGACATCCTCACCAATTTTG+AGG - chr5.1:4654148-4654167 MS.gene20350:intron 40.0%
! TGATGTTGGTGGGAACATAA+AGG - chr5.1:4655993-4656012 MS.gene20350:CDS 40.0%
! TTCTTGGCTCGTCATATAAC+TGG - chr5.1:4654681-4654700 MS.gene20350:intron 40.0%
!! ACATAGCTTGCTCAAACAAC+AGG - chr5.1:4653092-4653111 MS.gene20350:CDS 40.0%
!! AGACACGTATGTTTCGAGTT+AGG - chr5.1:4653860-4653879 MS.gene20350:CDS 40.0%
!! ATCCAAGTGGACTAATTAGC+AGG + chr5.1:4653759-4653778 None:intergenic 40.0%
!! GATTGGTCTATATCGTTGGT+TGG - chr5.1:4652651-4652670 MS.gene20350:CDS 40.0%
!! TGAAGTGCTGATTCCAAACA+GGG + chr5.1:4652196-4652215 None:intergenic 40.0%
!!! CCTTCCTGCTACTTTTGAAT+AGG - chr5.1:4652911-4652930 MS.gene20350:intron 40.0%
AACGCTCCTCAAAATTGGTG+AGG + chr5.1:4654157-4654176 None:intergenic 45.0%
CAAGATGCTCATGTTCCACA+AGG - chr5.1:4652527-4652546 MS.gene20350:intron 45.0%
CACCTGCTAATTAGTCCACT+TGG - chr5.1:4653754-4653773 MS.gene20350:CDS 45.0%
CAGGTGACCTATCAGATCTT+TGG + chr5.1:4653740-4653759 None:intergenic 45.0%
CCAAGCATGCTGTTAGAAGT+AGG + chr5.1:4652800-4652819 None:intergenic 45.0%
CCCTTTGAATCAACCTCAAG+TGG + chr5.1:4655937-4655956 None:intergenic 45.0%
CCTACTTCTAACAGCATGCT+TGG - chr5.1:4652797-4652816 MS.gene20350:intron 45.0%
CTATGGAGTGAACACATAGG+AGG - chr5.1:4655781-4655800 MS.gene20350:CDS 45.0%
CTCACACATTCAATGCTCTC+TGG + chr5.1:4655829-4655848 None:intergenic 45.0%
CTCCGACACATTCTCTCTTT+AGG - chr5.1:4653502-4653521 MS.gene20350:intron 45.0%
CTCCTATGTGTTCACTCCAT+AGG + chr5.1:4655783-4655802 None:intergenic 45.0%
CTCTGACATTATCGGCATTC+GGG - chr5.1:4654256-4654275 MS.gene20350:intron 45.0%
CTCTGAGTAGCTGTACTTGA+GGG + chr5.1:4655071-4655090 None:intergenic 45.0%
CTTCCTAGTTTGAGGGTTGA+TGG - chr5.1:4652554-4652573 MS.gene20350:intron 45.0%
GAAACATTCCCTGATGTTGG+TGG - chr5.1:4655982-4656001 MS.gene20350:CDS 45.0%
GAAATCTAAACCTCATGGCC+AGG - chr5.1:4655738-4655757 MS.gene20350:CDS 45.0%
GAGCCTACCAAAGATCTGAT+AGG - chr5.1:4653730-4653749 MS.gene20350:intron 45.0%
GGCATTGTAAAGCCTCTTGT+TGG - chr5.1:4655955-4655974 MS.gene20350:CDS 45.0%
TCACGTCAATACTGGACATC+TGG + chr5.1:4653788-4653807 None:intergenic 45.0%
TCCCTATGGAGTGAACACAT+AGG - chr5.1:4655778-4655797 MS.gene20350:CDS 45.0%
TGCTGGACAAAACAATCCTC+AGG - chr5.1:4655443-4655462 MS.gene20350:CDS 45.0%
! CCCACTTGAGGTTGATTCAA+AGG - chr5.1:4655933-4655952 MS.gene20350:CDS 45.0%
! GTCCTTTTCCTCCAGATCTT+GGG + chr5.1:4653074-4653093 None:intergenic 45.0%
! TCAGAGGATTCTCTTTTGGC+AGG - chr5.1:4655085-4655104 MS.gene20350:intron 45.0%
! TCTGAAGCAAGATGTGCAAC+AGG + chr5.1:4652224-4652243 None:intergenic 45.0%
! TGTCCTTTTCCTCCAGATCT+TGG + chr5.1:4653075-4653094 None:intergenic 45.0%
!! AGATGTCCAGTATTGACGTG+AGG - chr5.1:4653787-4653806 MS.gene20350:CDS 45.0%
!! CGTCGATTGGTCTATATCGT+TGG - chr5.1:4652647-4652666 MS.gene20350:CDS 45.0%
!! GAGAGTGCTTCTACTTGTGT+GGG - chr5.1:4652769-4652788 MS.gene20350:CDS 45.0%
!! GTGAAGTGCTGATTCCAAAC+AGG + chr5.1:4652197-4652216 None:intergenic 45.0%
!! TCACCAATTTTGAGGAGCGT+TGG - chr5.1:4654156-4654175 MS.gene20350:intron 45.0%
!! TGAGAGTGCTTCTACTTGTG+TGG - chr5.1:4652768-4652787 MS.gene20350:CDS 45.0%
!!! GGTGTTGGTGGTGATTTTCA+AGG - chr5.1:4652452-4652471 MS.gene20350:CDS 45.0%
!! ATATATTATAACATATTTAA+AGG + chr5.1:4654887-4654906 None:intergenic 5.0%
!! TATATTATAACATATTTAAA+GGG + chr5.1:4654886-4654905 None:intergenic 5.0%
!! TTAAATATGTTATAATATAT+AGG - chr5.1:4654887-4654906 MS.gene20350:intron 5.0%
AGGCCCGTCACAGAGTTTAA+TGG - chr5.1:4653929-4653948 MS.gene20350:CDS 50.0%
CAAGGAGACTTGGCATGTTC+AGG - chr5.1:4654304-4654323 MS.gene20350:intron 50.0%
CCACCATCAACCCTCAAACT+AGG + chr5.1:4652560-4652579 None:intergenic 50.0%
CCTCAAGTACAGCTACTCAG+AGG - chr5.1:4655069-4655088 MS.gene20350:intron 50.0%
CCTCTGAGTAGCTGTACTTG+AGG + chr5.1:4655072-4655091 None:intergenic 50.0%
CTGGTTGTCCAAGACAACCA+TGG - chr5.1:4654087-4654106 MS.gene20350:intron 50.0%
CTTCTTTGCCCAAGATCTGG+AGG - chr5.1:4653063-4653082 MS.gene20350:CDS 50.0%
GAGTGAACACATAGGAGGTG+TGG - chr5.1:4655786-4655805 MS.gene20350:CDS 50.0%
GCAGTCCTCACCATACAATC+CGG - chr5.1:4653672-4653691 MS.gene20350:intron 50.0%
GTCATTGTGGATGCTGATGC+TGG - chr5.1:4653414-4653433 MS.gene20350:CDS 50.0%
GTGATTCCTATGTGGCCTGA+AGG - chr5.1:4655041-4655060 MS.gene20350:CDS 50.0%
GTGCTTCTTTGCCCAAGATC+TGG - chr5.1:4653060-4653079 MS.gene20350:CDS 50.0%
TGCCCAAGATCTGGAGGAAA+AGG - chr5.1:4653069-4653088 MS.gene20350:CDS 50.0%
! AATAAGTCAGGCGCGTCGAT+TGG - chr5.1:4652634-4652653 MS.gene20350:CDS 50.0%
! GTACTTGAGGGTACACCTTC+AGG + chr5.1:4655059-4655078 None:intergenic 50.0%
! TACAAGGCATTAGAGGAGGC+TGG - chr5.1:4655321-4655340 MS.gene20350:CDS 50.0%
!! CCTAGTTTGAGGGTTGATGG+TGG - chr5.1:4652557-4652576 MS.gene20350:intron 50.0%
AAACCTCATGGCCAGGTGCA+TGG - chr5.1:4655745-4655764 MS.gene20350:CDS 55.0%
CAGGTGCCTCACGTCAATAC+TGG + chr5.1:4653796-4653815 None:intergenic 55.0%
GGCAGCAGGACCATCAACTT+TGG + chr5.1:4654126-4654145 None:intergenic 55.0%
GGTACACCTTCAGGCCACAT+AGG + chr5.1:4655050-4655069 None:intergenic 55.0%
GTGAGGATGTCATAGGCAGC+AGG + chr5.1:4654140-4654159 None:intergenic 55.0%
TAACCATGCACCTGGCCATG+AGG + chr5.1:4655751-4655770 None:intergenic 55.0%
! AGTAGCAGGAAGGCATGCCA+AGG + chr5.1:4652904-4652923 None:intergenic 55.0%
! CATCTGGAGCGTGATCCAAG+TGG + chr5.1:4653772-4653791 None:intergenic 55.0%
! GTAGCAGGAAGGCATGCCAA+GGG + chr5.1:4652903-4652922 None:intergenic 55.0%
CGGCGAGCTCGCGATTGCCA+AGG + chr5.1:4655392-4655411 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 4652023 4656053 4652023 ID=MS.gene20350
chr5.1 mRNA 4652023 4656053 4652023 ID=MS.gene20350.t1;Parent=MS.gene20350
chr5.1 exon 4655247 4656053 4655247 ID=MS.gene20350.t1.exon1;Parent=MS.gene20350.t1
chr5.1 CDS 4655247 4656053 4655247 ID=cds.MS.gene20350.t1;Parent=MS.gene20350.t1
chr5.1 exon 4654963 4655079 4654963 ID=MS.gene20350.t1.exon2;Parent=MS.gene20350.t1
chr5.1 CDS 4654963 4655079 4654963 ID=cds.MS.gene20350.t1;Parent=MS.gene20350.t1
chr5.1 exon 4654504 4654698 4654504 ID=MS.gene20350.t1.exon3;Parent=MS.gene20350.t1
chr5.1 CDS 4654504 4654698 4654504 ID=cds.MS.gene20350.t1;Parent=MS.gene20350.t1
chr5.1 exon 4653751 4653999 4653751 ID=MS.gene20350.t1.exon4;Parent=MS.gene20350.t1
chr5.1 CDS 4653751 4653999 4653751 ID=cds.MS.gene20350.t1;Parent=MS.gene20350.t1
chr5.1 exon 4653592 4653660 4653592 ID=MS.gene20350.t1.exon5;Parent=MS.gene20350.t1
chr5.1 CDS 4653592 4653660 4653592 ID=cds.MS.gene20350.t1;Parent=MS.gene20350.t1
chr5.1 exon 4653354 4653501 4653354 ID=MS.gene20350.t1.exon6;Parent=MS.gene20350.t1
chr5.1 CDS 4653354 4653501 4653354 ID=cds.MS.gene20350.t1;Parent=MS.gene20350.t1
chr5.1 exon 4652970 4653118 4652970 ID=MS.gene20350.t1.exon7;Parent=MS.gene20350.t1
chr5.1 CDS 4652970 4653118 4652970 ID=cds.MS.gene20350.t1;Parent=MS.gene20350.t1
chr5.1 exon 4652612 4652788 4652612 ID=MS.gene20350.t1.exon8;Parent=MS.gene20350.t1
chr5.1 CDS 4652612 4652788 4652612 ID=cds.MS.gene20350.t1;Parent=MS.gene20350.t1
chr5.1 exon 4652023 4652514 4652023 ID=MS.gene20350.t1.exon9;Parent=MS.gene20350.t1
chr5.1 CDS 4652023 4652514 4652023 ID=cds.MS.gene20350.t1;Parent=MS.gene20350.t1
Gene Sequence

>MS.gene20350

ATGGACACTTTTCACAAGAAAGTGGGGGAAATGTTTTCAGTCTTACCTAAAAAACTTGGTGGCAAAATTGAAGGCAAAATGTCTCGTAATATAACCAGTGATCCGTATGTAACTGTTTCTGTAGCTGGTGCTGTTATTGCAAGAACCTTTGTAATTAGAAACAATGAGAACCCTGTTTGGAATCAGCACTTCACTGTACCTGTTGCACATCTTGCTTCAGAAATTCACTTTGTTGTAAAAGACAATGATATTGTTGGTTCACAGATTATTGGTGCAGTTGGAATTCCAGTTGAAAAATTATGTCATGATACAAAAGTTGAAGGCTTTTTTCCCATTCTCAATACTAATGGAAAACCCTTTAAGACACAAACAATTTTGAACTTGTCAATTCAGTATACACCTGTTGATAAGGTAGCCCTTTATAGTAATGGTGTTGGTGGTGATTTTCAAGGTGTTCCTGCTACATATTTTCCACTTAGAAAAGGTGGAAAAGTTACACTTTATCAAGATGCTCATGTTCCACAAGGTTGTCTTCCTAGTTTGAGGGTTGATGGTGGTGATCATGATGTGAGTTATGAGAATGGAAATTGTTGGTATGATATCTTCGATGCAATAAGTCAGGCGCGTCGATTGGTCTATATCGTTGGTTGGTCCGTGTACTACAATGTTAGTCTCATTAGGGATACTAGAGATGGAAAAGATTGTACTTTAGGTGATCTTCTCAAAGCTAAATCACAGGAAGGTGTGAGAGTGCTTCTACTTGTGTGGGATGATCCTACTTCTAACAGCATGCTTGGATATAAAACAGTAATGATCTTCAAAAAAACCTTTTTATTTATGTTTCTGATATTTGGCTCTCTAACTCTATTAGTCTATTCCCTTGGCATGCCTTCCTGCTACTTTTGAATAGGCAATTAATTTGAAACCAGTTGCTTACTTCTATTACCGAATTACCATGATTTATTGATATGTAGGTTGGACTTATGAACACTTATGACGAGGAAACTCGCAGTTTTTTCAAGCACTCTTCAGTTAGGGTGCTTCTTTGCCCAAGATCTGGAGGAAAAGGACATAGCTTGCTCAAACAACAGGTCAGTTAGAGAAAAACTATTTTTGTATGTCTAACTGCATAGTACTGAATTACACAAAATGCTTGCAATTCTGTTATTTCTGGTTTTACTTATCTGCTTTATATTTCTTGTATACATCTTTTGTCTAAAAAAATATGTATTATATGTATCATATTTTAGAAATTGAAAACATGAGTACTCTATACAGTTTAATCAGATAATAGAAATAGTTAAAGGTGCAATCTTTTTAAAAAGGACATATTTTGCTTTTTGCTTAATTGTCAGGAAGCTGGAACAATCTATACGCATCATCAGAAGACAGTCATTGTGGATGCTGATGCTGGTCAGCATAAAAGAAAGATTATAGCTTTCATTGGAGGACTTGATTTATGTCTCGGAAGATATGATACTCCGACACATTCTCTCTTTAGGACTTTGCAGACCACACACAAAGATGACTTTCATAATCCTAACTTTGTGGTTAGTTATCCATTTTTTATTTTCATTAGTTTTCATTGCCGGTGTTAAAAGTATGGCATTAAGTGAGACATGGTTTTAAAATGTGTTTACACATGATGGGCAGTCCTCACCATACAATCCGGTTTTATAAGCTTTAGCTAGGCTCGAGTTGATATCAGAGCCTACCAAAGATCTGATAGGTCACCTGCTAATTAGTCCACTTGGATCACGCTCCAGATGTCCAGTATTGACGTGAGGCACCTGAGTTAAGAGTCTGTAGAATTGAATTAGGCATAACCGAAATTCTAAGACACGTATGTTTCGAGTTAGGCACCTTCGCATTTTTAATCTCCATAGACTGCTTTTTCTTTTTCAAAAGGCCCGTCACAGAGTTTAATGGTTGAGAATACATAATCAATTTCAAAATTTCTAACCATTTTATATGTTGTCCTTTGCGTTGTAATTGTTTTATTTGTCATAATAGAGGTTATGAATGAACTGAGTTTTAATTTGCATACTAAACAGGGTTCTGTTACTGGTTGTCCAAGACAACCATGGCATGATTTGCATTCCAAAGTTGATGGTCCTGCTGCCTATGACATCCTCACCAATTTTGAGGAGCGTTGGTTAAGAGCACTAAAAATGAATACACTTCAAAAGATGAAAACTTCACATGATGATTCATTACTGAAAATTGACAGAATCTCTGACATTATCGGCATTCGGGAAGTACCTTGCCTTGATGAGCATAACAAGGAGACTTGGCATGTTCAGGTATCATAGACTGATTTAATTCTCATTCCCTTAAAGAAACTTCTTCAATTTTGATGTATCTAATTGTTTATTATATCCTTTAAATTGAAGATCTTCCGTTCTATTGATTCCAATTCTGTGAAAGGATTTCCAAAAGAACCAAAAGATGCAATACAAAGGGTAAAGTGACTTCATTCCATTCTCAAATTTAGATTTTTTGTTATGTTAGGTGTGAAACAAAGAGCTGACTATGCTTACTTCATTGAACAGAACTTGGTTTGTGGAAAAAATGTAATGATAGACATGAGCATACATTCTGCATATGTCAAAGCAATCAGAGCAGCACAGAAGTTCATTTATATTGAGAATCAATATTTTCTTGGCTCGTCATATAACTGGGATTCTTTCAAAGACCTCGGTGAGTATTGAGTTCTAAAACCAATAAAATGATACTCCATCTGTTCCACAATAATGGAGATATTTGACTGTTTCTTACAGATTAAGAAAAGTGTGTAAATATAAGAGTGAAAATAATAATACTTTTATTAAATTACTCTTATCAAGTATTTATGTATTTACCCTTTAAATATGTTATAATATATAGGTTGCAACAGGCATCCTTGTTATATTCATTCCTTTTCTTTTATTCACAGGTGCAAACAACTTAATTCCGATGGAAATTGCGCTAAAAATAGCCAACAAAATCAAACAGAAAGAAAGATTCTGTGTGTATATTGTGATTCCTATGTGGCCTGAAGGTGTACCCTCAAGTACAGCTACTCAGAGGATTCTCTTTTGGCAGGTAAGCTTCCTTTACCACTTAAAATGGTGTCATGTTATAAATCATAAAAATTTCTATTTTGATGGCATAATGATTAAGAAGATTTTCTTCTCAAACATGTATATTAAGCTAGAAGAAACTCTCAAATTTGTGTGCTTTGTATTATCAAATTATCCTAAACTCTTAAATGTTGATTTGGTAGTTCAAAACAATGCAAATGATGTATGGAACAATTTACAAGGCATTAGAGGAGGCTGGACTTGAAAATGAGTATGAACCACAGGACTACTTGAATTTCTTTTGCCTTGGCAATCGCGAGCTCGCCGATAATGAAAACATTTCAAATGTTGTAAGAGCTGCTGGACAAAACAATCCTCAGGTATTTTGTTTTTACTCTCTTTAAGTAAACTTGAAATTTTTTGCCTTTTGATCAAGTTAGTTTTATCATTATTGTTTGCATATTTTGGAACCTATAGGTACTTACTCAAAAGCACAGAAGATTCATGATATATGTTCATTCAAAAGGAATGATAGTGGATGATGAATACGTGTTAATGGGATCTGCCAACATAAACCAAAGATCAATGGAAGGCACTAGAGACACAGAAATTGCAATGGGAGCATATCAGCCTAATCATACTTGGGCAACCAAGAAATCTAAACCTCATGGCCAGGTGCATGGTTATAGAATGTCCCTATGGAGTGAACACATAGGAGGTGTGGAAGAATGTTTCAAGCAACCAGAGAGCATTGAATGTGTGAGAAGAATAAGGTCATTGAGTGAGTATAACTGGAGACAATATTTGGCTGATGAAGTAACTGAAATGAATGCTCATTTACTGAAATACCCACTTGAGGTTGATTCAAAGGGCATTGTAAAGCCTCTTGTTGGCTGTGAAACATTCCCTGATGTTGGTGGGAACATAAAGGGCACCTTTACTGTACTTCAAGAAAATCTCACCATATAA
Protein sequence

>MS.gene20350.t1

MDTFHKKVGEMFSVLPKKLGGKIEGKMSRNITSDPYVTVSVAGAVIARTFVIRNNENPVWNQHFTVPVAHLASEIHFVVKDNDIVGSQIIGAVGIPVEKLCHDTKVEGFFPILNTNGKPFKTQTILNLSIQYTPVDKVALYSNGVGGDFQGVPATYFPLRKGGKVTLYQDAHVPQGCLPSLRVDGGDHDVSYENGNCWYDIFDAISQARRLVYIVGWSVYYNVSLIRDTRDGKDCTLGDLLKAKSQEGVRVLLLVWDDPTSNSMLGYKTVGLMNTYDEETRSFFKHSSVRVLLCPRSGGKGHSLLKQQEAGTIYTHHQKTVIVDADAGQHKRKIIAFIGGLDLCLGRYDTPTHSLFRTLQTTHKDDFHNPNFVGSVTGCPRQPWHDLHSKVDGPAAYDILTNFEERWLRALKMNTLQKMKTSHDDSLLKIDRISDIIGIREVPCLDEHNKETWHVQIFRSIDSNSVKGFPKEPKDAIQRNLVCGKNVMIDMSIHSAYVKAIRAAQKFIYIENQYFLGSSYNWDSFKDLGANNLIPMEIALKIANKIKQKERFCVYIVIPMWPEGVPSSTATQRILFWQFKTMQMMYGTIYKALEEAGLENEYEPQDYLNFFCLGNRELADNENISNVVRAAGQNNPQVLTQKHRRFMIYVHSKGMIVDDEYVLMGSANINQRSMEGTRDTEIAMGAYQPNHTWATKKSKPHGQVHGYRMSLWSEHIGGVEECFKQPESIECVRRIRSLSEYNWRQYLADEVTEMNAHLLKYPLEVDSKGIVKPLVGCETFPDVGGNIKGTFTVLQENLTI