Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20887.t1 | AES61601.1 | 80.2 | 116 | 21 | 2 | 1 | 114 | 1 | 116 | 6.20E-35 | 156.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20887.t1 | G7ICT0 | 80.2 | 116 | 21 | 2 | 1 | 114 | 1 | 116 | 4.4e-35 | 156.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050780 | MS.gene20887 | 0.838474 | 2.81E-57 | -1.69E-46 |
| MS.gene051492 | MS.gene20887 | 0.821703 | 3.51E-53 | -1.69E-46 |
| MS.gene051493 | MS.gene20887 | 0.827828 | 1.26E-54 | -1.69E-46 |
| MS.gene051494 | MS.gene20887 | 0.842831 | 2.03E-58 | -1.69E-46 |
| MS.gene053641 | MS.gene20887 | 0.836774 | 7.68E-57 | -1.69E-46 |
| MS.gene05468 | MS.gene20887 | 0.804839 | 1.78E-49 | -1.69E-46 |
| MS.gene058477 | MS.gene20887 | 0.806673 | 7.34E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20887.t1 | MTR_1g086450 | 83.621 | 116 | 17 | 2 | 1 | 114 | 1 | 116 | 1.40e-44 | 140 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGAGGAGGAAGAAGAAGATA+TGG | 0.309577 | 1.3:+61246157 | MS.gene20887:CDS |
| CACGTAGTTCTAATTCTCTT+TGG | 0.313385 | 1.3:-61246238 | None:intergenic |
| CTTCCTCCTCGTTCTTATTG+AGG | 0.398320 | 1.3:-61246145 | None:intergenic |
| CTCTTTGGCGTAGTCGATCA+CGG | 0.421114 | 1.3:-61246223 | None:intergenic |
| ACGATTAAAGAGCAAGTTTG+AGG | 0.460676 | 1.3:-61246439 | None:intergenic |
| AAAGAGCAAGTTTGAGGTCA+AGG | 0.490588 | 1.3:-61246433 | None:intergenic |
| ATTAGAACTACGTGATCAGC+TGG | 0.493231 | 1.3:+61246247 | MS.gene20887:CDS |
| AAATAACAAAGTTGAGAAGA+AGG | 0.516605 | 1.3:+61246403 | MS.gene20887:CDS |
| CTGGGTTGATAAAATTCAAA+GGG | 0.533763 | 1.3:-61246358 | None:intergenic |
| TCTTTGGCGTAGTCGATCAC+GGG | 0.543652 | 1.3:-61246222 | None:intergenic |
| ACTGGGTTGATAAAATTCAA+AGG | 0.551121 | 1.3:-61246359 | None:intergenic |
| AAGAGCAAGTTTGAGGTCAA+GGG | 0.558615 | 1.3:-61246432 | None:intergenic |
| AAGTCCTGCAATTCAAAACA+AGG | 0.614434 | 1.3:-61246308 | None:intergenic |
| GTAGTCGATCACGGGCGTCA+CGG | 0.615898 | 1.3:-61246214 | None:intergenic |
| TGTCCTCAATAAGAACGAGG+AGG | 0.708771 | 1.3:+61246142 | MS.gene20887:CDS |
| TAATGTCCTCAATAAGAACG+AGG | 0.718289 | 1.3:+61246139 | MS.gene20887:CDS |
| TATGGAAAACAAGAAGAACG+AGG | 0.749778 | 1.3:+61246112 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATAACAAAGTTGAGAAGA+AGG | + | chr1.3:61246403-61246422 | MS.gene20887:CDS | 25.0% |
| !! | ACTGGGTTGATAAAATTCAA+AGG | - | chr1.3:61246362-61246381 | None:intergenic | 30.0% |
| !! | ATGATTTTGTCATTTGCAAC+TGG | - | chr1.3:61246380-61246399 | None:intergenic | 30.0% |
| !! | CTGGGTTGATAAAATTCAAA+GGG | - | chr1.3:61246361-61246380 | None:intergenic | 30.0% |
| !! | TGATTTTGTCATTTGCAACT+GGG | - | chr1.3:61246379-61246398 | None:intergenic | 30.0% |
| AAGTCCTGCAATTCAAAACA+AGG | - | chr1.3:61246311-61246330 | None:intergenic | 35.0% | |
| TAATGTCCTCAATAAGAACG+AGG | + | chr1.3:61246139-61246158 | MS.gene20887:CDS | 35.0% | |
| !! | CACGTAGTTCTAATTCTCTT+TGG | - | chr1.3:61246241-61246260 | None:intergenic | 35.0% |
| ATTAGAACTACGTGATCAGC+TGG | + | chr1.3:61246247-61246266 | MS.gene20887:CDS | 40.0% | |
| ! | AAAGAGCAAGTTTGAGGTCA+AGG | - | chr1.3:61246436-61246455 | None:intergenic | 40.0% |
| ! | AAGAGCAAGTTTGAGGTCAA+GGG | - | chr1.3:61246435-61246454 | None:intergenic | 40.0% |
| !!! | AGAGCCTTGTTTTGAATTGC+AGG | + | chr1.3:61246304-61246323 | MS.gene20887:CDS | 40.0% |
| CGAGGAGGAAGAAGAAGATA+TGG | + | chr1.3:61246157-61246176 | MS.gene20887:CDS | 45.0% | |
| CTTCCTCCTCGTTCTTATTG+AGG | - | chr1.3:61246148-61246167 | None:intergenic | 45.0% | |
| TGTCCTCAATAAGAACGAGG+AGG | + | chr1.3:61246142-61246161 | MS.gene20887:CDS | 45.0% | |
| CTCTTTGGCGTAGTCGATCA+CGG | - | chr1.3:61246226-61246245 | None:intergenic | 50.0% | |
| TCTTTGGCGTAGTCGATCAC+GGG | - | chr1.3:61246225-61246244 | None:intergenic | 50.0% | |
| GTAGTCGATCACGGGCGTCA+CGG | - | chr1.3:61246217-61246236 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 61246113 | 61246457 | 61246113 | ID=MS.gene20887 |
| chr1.3 | mRNA | 61246113 | 61246457 | 61246113 | ID=MS.gene20887.t1;Parent=MS.gene20887 |
| chr1.3 | exon | 61246113 | 61246457 | 61246113 | ID=MS.gene20887.t1.exon1;Parent=MS.gene20887.t1 |
| chr1.3 | CDS | 61246113 | 61246457 | 61246113 | ID=cds.MS.gene20887.t1;Parent=MS.gene20887.t1 |
| Gene Sequence |
| Protein sequence |
