Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20964.t1 | AES61501.1 | 95 | 80 | 4 | 0 | 1 | 80 | 1 | 80 | 2.70E-37 | 164.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20964.t1 | G7IAC0 | 95.0 | 80 | 4 | 0 | 1 | 80 | 1 | 80 | 2.0e-37 | 164.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050563 | MS.gene20964 | 0.829606 | 4.69E-55 | -1.69E-46 |
| MS.gene052456 | MS.gene20964 | 0.8051 | 1.57E-49 | -1.69E-46 |
| MS.gene053383 | MS.gene20964 | 0.869491 | 2.98E-66 | -1.69E-46 |
| MS.gene05456 | MS.gene20964 | 0.803395 | 3.56E-49 | -1.69E-46 |
| MS.gene055359 | MS.gene20964 | 0.842968 | 1.87E-58 | -1.69E-46 |
| MS.gene059006 | MS.gene20964 | 0.810919 | 9.07E-51 | -1.69E-46 |
| MS.gene060513 | MS.gene20964 | 0.814832 | 1.26E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20964.t1 | MTR_1g084920 | 93.827 | 81 | 5 | 0 | 1 | 81 | 1 | 81 | 4.64e-53 | 159 |
| MS.gene20964.t1 | MTR_0187s0020 | 84.146 | 82 | 11 | 1 | 1 | 80 | 1 | 82 | 3.84e-45 | 139 |
| MS.gene20964.t1 | MTR_1g084840 | 80.723 | 83 | 14 | 1 | 1 | 81 | 1 | 83 | 9.29e-43 | 134 |
| MS.gene20964.t1 | MTR_0187s0050 | 77.108 | 83 | 17 | 1 | 1 | 81 | 1 | 83 | 2.29e-41 | 130 |
| MS.gene20964.t1 | MTR_0187s0070 | 77.108 | 83 | 17 | 1 | 1 | 81 | 1 | 83 | 2.29e-41 | 130 |
| MS.gene20964.t1 | MTR_1g485020 | 83.750 | 80 | 13 | 0 | 2 | 81 | 4 | 83 | 7.18e-36 | 116 |
| MS.gene20964.t1 | MTR_1g484990 | 81.481 | 81 | 15 | 0 | 1 | 81 | 2 | 82 | 8.56e-36 | 116 |
| MS.gene20964.t1 | MTR_7g111500 | 48.101 | 79 | 38 | 2 | 1 | 76 | 1 | 79 | 5.23e-19 | 73.9 |
| MS.gene20964.t1 | MTR_1g485000 | 56.923 | 65 | 22 | 3 | 15 | 73 | 18 | 82 | 2.85e-17 | 69.7 |
| MS.gene20964.t1 | MTR_1g484970 | 56.923 | 65 | 22 | 3 | 15 | 73 | 18 | 82 | 2.85e-17 | 69.7 |
| MS.gene20964.t1 | MTR_1g084900 | 56.923 | 65 | 22 | 3 | 15 | 73 | 19 | 83 | 3.34e-17 | 69.7 |
| MS.gene20964.t1 | MTR_0187s0080 | 56.923 | 65 | 22 | 3 | 15 | 73 | 19 | 83 | 3.48e-17 | 69.7 |
| MS.gene20964.t1 | MTR_1g084830 | 56.923 | 65 | 22 | 3 | 15 | 73 | 18 | 82 | 4.12e-16 | 68.6 |
| MS.gene20964.t1 | MTR_0187s0030 | 59.016 | 61 | 20 | 3 | 18 | 73 | 20 | 80 | 1.28e-15 | 65.5 |
| MS.gene20964.t1 | MTR_0187s0060 | 59.016 | 61 | 20 | 3 | 18 | 73 | 20 | 80 | 1.28e-15 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20964.t1 | AT3G05858 | 45.882 | 85 | 39 | 4 | 1 | 79 | 1 | 84 | 2.47e-12 | 57.4 |
Find 16 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGATGAAGACAAAGGTAAC+TGG | 0.348340 | 1.3:+60375560 | MS.gene20964:CDS |
| GGTAAGACTAAGTCTTCTTC+AGG | 0.365971 | 1.3:+60375451 | MS.gene20964:CDS |
| TCAGCTCTCATGTTAATGTT+AGG | 0.378896 | 1.3:-60375595 | None:intergenic |
| AAACTCGATTCTTGTATTTG+CGG | 0.401356 | 1.3:-60375627 | None:intergenic |
| TGGATCAACTTCATAGCGTT+CGG | 0.459202 | 1.3:-60375656 | None:intergenic |
| TTACCTTTGTCTTCATCACT+AGG | 0.502543 | 1.3:-60375556 | None:intergenic |
| TATTATGCATTCTAGACCGC+TGG | 0.510025 | 1.3:-60375676 | None:intergenic |
| TCAAAGAATAAGTCTAGAGC+AGG | 0.540317 | 1.3:+60375502 | MS.gene20964:CDS |
| TGATGAAGACAAAGGTAACT+GGG | 0.585582 | 1.3:+60375561 | MS.gene20964:CDS |
| GCATTAATCATCATGGGAGG+TGG | 0.588766 | 1.3:+60375430 | None:intergenic |
| GCAGGATACTATGATGATTG+CGG | 0.626514 | 1.3:+60375520 | MS.gene20964:CDS |
| ATGATTGCGGATGCAAGACA+TGG | 0.656100 | 1.3:+60375533 | MS.gene20964:CDS |
| ACGCTATGAAGTTGATCCAG+CGG | 0.687155 | 1.3:+60375660 | MS.gene20964:CDS |
| TGGCCTAGTGATGAAGACAA+AGG | 0.708445 | 1.3:+60375553 | MS.gene20964:CDS |
| GCAGCATTAATCATCATGGG+AGG | 0.708531 | 1.3:+60375427 | None:intergenic |
| GATGAAGACAAAGGTAACTG+GGG | 0.719672 | 1.3:+60375562 | MS.gene20964:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACTCGATTCTTGTATTTG+CGG | - | chr1.3:60375630-60375649 | None:intergenic | 30.0% | |
| TCAGCTCTCATGTTAATGTT+AGG | - | chr1.3:60375598-60375617 | None:intergenic | 35.0% | |
| TGATGAAGACAAAGGTAACT+GGG | + | chr1.3:60375561-60375580 | MS.gene20964:CDS | 35.0% | |
| TTACCTTTGTCTTCATCACT+AGG | - | chr1.3:60375559-60375578 | None:intergenic | 35.0% | |
| ! | TCAAAGAATAAGTCTAGAGC+AGG | + | chr1.3:60375502-60375521 | MS.gene20964:CDS | 35.0% |
| GATGAAGACAAAGGTAACTG+GGG | + | chr1.3:60375562-60375581 | MS.gene20964:CDS | 40.0% | |
| GCAGGATACTATGATGATTG+CGG | + | chr1.3:60375520-60375539 | MS.gene20964:CDS | 40.0% | |
| GGTAAGACTAAGTCTTCTTC+AGG | + | chr1.3:60375451-60375470 | MS.gene20964:CDS | 40.0% | |
| GTGATGAAGACAAAGGTAAC+TGG | + | chr1.3:60375560-60375579 | MS.gene20964:CDS | 40.0% | |
| TGGATCAACTTCATAGCGTT+CGG | - | chr1.3:60375659-60375678 | None:intergenic | 40.0% | |
| ATGATTGCGGATGCAAGACA+TGG | + | chr1.3:60375533-60375552 | MS.gene20964:CDS | 45.0% | |
| TGGCCTAGTGATGAAGACAA+AGG | + | chr1.3:60375553-60375572 | MS.gene20964:CDS | 45.0% | |
| ! | ACGCTATGAAGTTGATCCAG+CGG | + | chr1.3:60375660-60375679 | MS.gene20964:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 60375442 | 60375687 | 60375442 | ID=MS.gene20964 |
| chr1.3 | mRNA | 60375442 | 60375687 | 60375442 | ID=MS.gene20964.t1;Parent=MS.gene20964 |
| chr1.3 | exon | 60375442 | 60375687 | 60375442 | ID=MS.gene20964.t1.exon1;Parent=MS.gene20964.t1 |
| chr1.3 | CDS | 60375442 | 60375687 | 60375442 | ID=cds.MS.gene20964.t1;Parent=MS.gene20964.t1 |
| Gene Sequence |
| Protein sequence |
