Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060513.t1 | XP_003627523.2 | 96 | 276 | 11 | 0 | 1 | 276 | 1 | 276 | 8.50E-147 | 529.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060513.t1 | G7LAU0 | 96.0 | 276 | 11 | 0 | 1 | 276 | 1 | 276 | 6.1e-147 | 529.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050584 | MS.gene060513 | 0.805137 | 1.54E-49 | -1.69E-46 |
| MS.gene051789 | MS.gene060513 | 0.801711 | 7.92E-49 | -1.69E-46 |
| MS.gene053074 | MS.gene060513 | 0.804655 | 1.95E-49 | -1.69E-46 |
| MS.gene053383 | MS.gene060513 | 0.821069 | 4.91E-53 | -1.69E-46 |
| MS.gene05456 | MS.gene060513 | 0.807287 | 5.44E-50 | -1.69E-46 |
| MS.gene055359 | MS.gene060513 | 0.812022 | 5.23E-51 | -1.69E-46 |
| MS.gene059006 | MS.gene060513 | 0.811193 | 7.91E-51 | -1.69E-46 |
| MS.gene060513 | MS.gene061336 | 0.802693 | 4.97E-49 | -1.69E-46 |
| MS.gene060513 | MS.gene063566 | 0.81532 | 9.82E-52 | -1.69E-46 |
| MS.gene060513 | MS.gene067305 | 0.870278 | 1.65E-66 | -1.69E-46 |
| MS.gene060513 | MS.gene069330 | 0.803756 | 3.00E-49 | -1.69E-46 |
| MS.gene060513 | MS.gene070489 | 0.812683 | 3.75E-51 | -1.69E-46 |
| MS.gene060513 | MS.gene20964 | 0.814832 | 1.26E-51 | -1.69E-46 |
| MS.gene060513 | MS.gene22109 | 0.822556 | 2.22E-53 | -1.69E-46 |
| MS.gene060513 | MS.gene22855 | 0.806558 | 7.76E-50 | -1.69E-46 |
| MS.gene060513 | MS.gene22856 | 0.812666 | 3.78E-51 | -1.69E-46 |
| MS.gene060513 | MS.gene22900 | 0.828276 | 9.84E-55 | -1.69E-46 |
| MS.gene060513 | MS.gene23012 | 0.832455 | 9.36E-56 | -1.69E-46 |
| MS.gene060513 | MS.gene26076 | 0.804808 | 1.81E-49 | -1.69E-46 |
| MS.gene060513 | MS.gene35428 | 0.805164 | 1.52E-49 | -1.69E-46 |
| MS.gene060513 | MS.gene35695 | 0.80895 | 2.41E-50 | -1.69E-46 |
| MS.gene060513 | MS.gene37909 | 0.822467 | 2.33E-53 | -1.69E-46 |
| MS.gene060513 | MS.gene65661 | 0.807877 | 4.08E-50 | -1.69E-46 |
| MS.gene060513 | MS.gene69587 | 0.816887 | 4.39E-52 | -1.69E-46 |
| MS.gene060513 | MS.gene76705 | 0.807267 | 5.50E-50 | -1.69E-46 |
| MS.gene060513 | MS.gene80842 | 0.802824 | 4.67E-49 | -1.69E-46 |
| MS.gene060513 | MS.gene81601 | 0.804946 | 1.69E-49 | -1.69E-46 |
| MS.gene060513 | MS.gene82214 | 0.801393 | 9.20E-49 | -1.69E-46 |
| MS.gene060513 | MS.gene82571 | 0.802196 | 6.29E-49 | -1.69E-46 |
| MS.gene060513 | MS.gene83221 | 0.815139 | 1.08E-51 | -1.69E-46 |
| MS.gene060513 | MS.gene84726 | 0.901477 | 2.58E-78 | -1.69E-46 |
| MS.gene060513 | MS.gene84833 | 0.873456 | 1.45E-67 | -1.69E-46 |
| MS.gene060513 | MS.gene89207 | 0.804881 | 1.75E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060513.t1 | MTR_8g027100 | 96.014 | 276 | 11 | 0 | 1 | 276 | 1 | 276 | 0.0 | 504 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060513.t1 | AT2G46640 | 32.963 | 270 | 140 | 13 | 1 | 250 | 1 | 249 | 9.51e-22 | 92.4 |
| MS.gene060513.t1 | AT2G46640 | 32.841 | 271 | 141 | 13 | 1 | 250 | 1 | 251 | 2.11e-20 | 88.6 |
| MS.gene060513.t1 | AT2G46640 | 32.841 | 271 | 141 | 13 | 1 | 250 | 1 | 251 | 2.39e-20 | 88.6 |
| MS.gene060513.t1 | AT2G46640 | 31.034 | 232 | 123 | 10 | 36 | 250 | 26 | 237 | 3.00e-15 | 74.3 |
Find 42 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATGATGATTAGAAACTTTA+AGG | 0.101915 | 8.1:+75305034 | MS.gene060513:CDS |
| ACTTTCTTGATGTCTTTAAT+TGG | 0.226702 | 8.1:-75305299 | None:intergenic |
| CTTCCAAAGCAAAGTATTCT+TGG | 0.268337 | 8.1:-75304839 | None:intergenic |
| GTATTCTTGGTGATTGTTCA+TGG | 0.327683 | 8.1:-75304826 | None:intergenic |
| CATGGTGTGATTTACCTTCA+TGG | 0.334098 | 8.1:-75304317 | None:intergenic |
| GAAGATTATTCCCCTTGTTA+AGG | 0.356621 | 8.1:+75305265 | MS.gene060513:CDS |
| GTTTGTTACAGATCTTCAAT+TGG | 0.412010 | 8.1:+75304477 | MS.gene060513:intron |
| TCACCAAGAATACTTTGCTT+TGG | 0.419615 | 8.1:+75304836 | MS.gene060513:CDS |
| CATCCTAACAACACTCTCAA+TGG | 0.422290 | 8.1:+75304515 | MS.gene060513:CDS |
| AAACCATTGAGAGTGTTGTT+AGG | 0.424249 | 8.1:-75304518 | None:intergenic |
| ATTCTTACCATTGGTACTCT+TGG | 0.429438 | 8.1:+75304786 | MS.gene060513:CDS |
| ACTTGACTCAACCAAGACTT+TGG | 0.432800 | 8.1:-75305198 | None:intergenic |
| TTTGTTACAGATCTTCAATT+GGG | 0.432814 | 8.1:+75304478 | MS.gene060513:intron |
| ATGATTTCATGACAAATCAT+AGG | 0.438088 | 8.1:-75305074 | None:intergenic |
| TCAAATGTGTTGTTGATCAA+TGG | 0.438577 | 8.1:-75304939 | None:intergenic |
| TTTCCAATCACCGAGCATAT+CGG | 0.445673 | 8.1:-75304757 | None:intergenic |
| TGACCGATATGCTCGGTGAT+TGG | 0.446300 | 8.1:+75304754 | MS.gene060513:CDS |
| TGATTCTTGACATCAAGATC+TGG | 0.455218 | 8.1:-75305663 | None:intergenic |
| AAACTTTAAGGAAATTATTG+AGG | 0.468461 | 8.1:+75305046 | MS.gene060513:CDS |
| ATTTGATGAGAACAACTTTG+AGG | 0.478699 | 8.1:+75304956 | MS.gene060513:CDS |
| TTATCAGTGTTGTTGATTGA+AGG | 0.480019 | 8.1:-75304705 | None:intergenic |
| ATTAATAACCACATGAATGA+AGG | 0.483710 | 8.1:+75305705 | MS.gene060513:CDS |
| TGATGAAGAAGATGCTAAAG+AGG | 0.486614 | 8.1:+75305631 | MS.gene060513:CDS |
| TATGATTTGTCATGAAATCA+TGG | 0.489589 | 8.1:+75305076 | MS.gene060513:CDS |
| TTGAATCTGATCAAAAGAAA+AGG | 0.490869 | 8.1:+75305111 | MS.gene060513:CDS |
| TGATGATGATGCTAGAAAAG+AGG | 0.505541 | 8.1:+75305007 | MS.gene060513:CDS |
| TTATTCTGCAGCCAAAGTCT+TGG | 0.511362 | 8.1:+75305187 | MS.gene060513:CDS |
| TTTCTATGATGTTGAACAAG+AGG | 0.511917 | 8.1:+75304908 | MS.gene060513:CDS |
| AGCCATTGCCTTCATTCATG+TGG | 0.516778 | 8.1:-75305713 | None:intergenic |
| TCTTCAATTGGGTGCACAAG+AGG | 0.532391 | 8.1:+75304489 | MS.gene060513:CDS |
| AAAGACGGAATTCTTACCAT+TGG | 0.543523 | 8.1:+75304777 | MS.gene060513:CDS |
| ATCATAGCCAAGAGTACCAA+TGG | 0.569824 | 8.1:-75304793 | None:intergenic |
| CATCATCATCAACTACATCA+TGG | 0.574853 | 8.1:-75304995 | None:intergenic |
| ATGCTCGGTGATTGGAAAGA+CGG | 0.581029 | 8.1:+75304762 | MS.gene060513:CDS |
| TCTTGACATCAAGATCTGGA+TGG | 0.589109 | 8.1:-75305659 | None:intergenic |
| TGAATGAAGGCAATGGCTCA+AGG | 0.597220 | 8.1:+75305718 | MS.gene060513:CDS |
| TGATTGTTCATGGATTTCAA+AGG | 0.604206 | 8.1:-75304816 | None:intergenic |
| AACCACATGAATGAAGGCAA+TGG | 0.612697 | 8.1:+75305711 | MS.gene060513:CDS |
| GTTGCTTTGACCGATATGCT+CGG | 0.632441 | 8.1:+75304747 | MS.gene060513:CDS |
| ATTTCATGACAAATCATAGG+TGG | 0.650243 | 8.1:-75305071 | None:intergenic |
| TCTTAACATCAGAATCAGCA+AGG | 0.653265 | 8.1:-75305154 | None:intergenic |
| GATTTGTCATGAAATCATGG+AGG | 0.748556 | 8.1:+75305079 | MS.gene060513:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTATTTGCTTGAAATTAAT+TGG | + | chr8.1:75305578-75305597 | MS.gene060513:intron | 15.0% |
| !!! | ATTTATATACATTTTCACAA+TGG | + | chr8.1:75305455-75305474 | MS.gene060513:intron | 15.0% |
| !!! | TTTATTTTAGACACAAAATT+TGG | - | chr8.1:75304590-75304609 | None:intergenic | 15.0% |
| !! | AATGATGATTAGAAACTTTA+AGG | + | chr8.1:75305034-75305053 | MS.gene060513:CDS | 20.0% |
| !! | TAATTACATATATAGCCTTT+AGG | - | chr8.1:75305351-75305370 | None:intergenic | 20.0% |
| !! | TCAAGCAAATAATTGATTAT+TGG | - | chr8.1:75305571-75305590 | None:intergenic | 20.0% |
| !!! | AAACTTTAAGGAAATTATTG+AGG | + | chr8.1:75305046-75305065 | MS.gene060513:CDS | 20.0% |
| !!! | CACAATTTTTAAGACAATAA+AGG | - | chr8.1:75304405-75304424 | None:intergenic | 20.0% |
| !!! | TGTGTCTAAAATAAAATCAA+TGG | + | chr8.1:75304595-75304614 | MS.gene060513:intron | 20.0% |
| ! | ACTTTCTTGATGTCTTTAAT+TGG | - | chr8.1:75305302-75305321 | None:intergenic | 25.0% |
| ! | ATGATTTCATGACAAATCAT+AGG | - | chr8.1:75305077-75305096 | None:intergenic | 25.0% |
| ! | ATTAATAACCACATGAATGA+AGG | + | chr8.1:75305705-75305724 | MS.gene060513:CDS | 25.0% |
| ! | CATATGCATGAGAAATATAA+TGG | - | chr8.1:75304357-75304376 | None:intergenic | 25.0% |
| ! | CCAAAACTTAAAGCAAAAAA+TGG | + | chr8.1:75305227-75305246 | MS.gene060513:CDS | 25.0% |
| ! | TTGAATCTGATCAAAAGAAA+AGG | + | chr8.1:75305111-75305130 | MS.gene060513:CDS | 25.0% |
| ! | TTGCTTGAAATTAATTGGTT+AGG | + | chr8.1:75305583-75305602 | MS.gene060513:intron | 25.0% |
| ! | TTTGTTACAGATCTTCAATT+GGG | + | chr8.1:75304478-75304497 | MS.gene060513:intron | 25.0% |
| !! | TATGATTTGTCATGAAATCA+TGG | + | chr8.1:75305076-75305095 | MS.gene060513:CDS | 25.0% |
| !!! | CCATTTTTTGCTTTAAGTTT+TGG | - | chr8.1:75305230-75305249 | None:intergenic | 25.0% |
| !!! | GTTTGTTGTTTTTACTTAGA+TGG | + | chr8.1:75304654-75304673 | MS.gene060513:intron | 25.0% |
| ATTTCATGACAAATCATAGG+TGG | - | chr8.1:75305074-75305093 | None:intergenic | 30.0% | |
| ATTTGATGAGAACAACTTTG+AGG | + | chr8.1:75304956-75304975 | MS.gene060513:CDS | 30.0% | |
| TGATTGTTCATGGATTTCAA+AGG | - | chr8.1:75304819-75304838 | None:intergenic | 30.0% | |
| TTTCTATGATGTTGAACAAG+AGG | + | chr8.1:75304908-75304927 | MS.gene060513:CDS | 30.0% | |
| ! | GTTTGTTACAGATCTTCAAT+TGG | + | chr8.1:75304477-75304496 | MS.gene060513:intron | 30.0% |
| ! | TCAAATGTGTTGTTGATCAA+TGG | - | chr8.1:75304942-75304961 | None:intergenic | 30.0% |
| ! | TTATCAGTGTTGTTGATTGA+AGG | - | chr8.1:75304708-75304727 | None:intergenic | 30.0% |
| AAAGACGGAATTCTTACCAT+TGG | + | chr8.1:75304777-75304796 | MS.gene060513:CDS | 35.0% | |
| ATTCTTACCATTGGTACTCT+TGG | + | chr8.1:75304786-75304805 | MS.gene060513:CDS | 35.0% | |
| CATCATCATCAACTACATCA+TGG | - | chr8.1:75304998-75305017 | None:intergenic | 35.0% | |
| GAAGATTATTCCCCTTGTTA+AGG | + | chr8.1:75305265-75305284 | MS.gene060513:CDS | 35.0% | |
| GATTTGTCATGAAATCATGG+AGG | + | chr8.1:75305079-75305098 | MS.gene060513:CDS | 35.0% | |
| TCACCAAGAATACTTTGCTT+TGG | + | chr8.1:75304836-75304855 | MS.gene060513:CDS | 35.0% | |
| TCTTAACATCAGAATCAGCA+AGG | - | chr8.1:75305157-75305176 | None:intergenic | 35.0% | |
| TGATGAAGAAGATGCTAAAG+AGG | + | chr8.1:75305631-75305650 | MS.gene060513:CDS | 35.0% | |
| TGATTCTTGACATCAAGATC+TGG | - | chr8.1:75305666-75305685 | None:intergenic | 35.0% | |
| TTGTAGATAGGATAGTGTTG+AGG | + | chr8.1:75305545-75305564 | MS.gene060513:intron | 35.0% | |
| ! | ACGTGCATTTTCCTTAACAA+GGG | - | chr8.1:75305279-75305298 | None:intergenic | 35.0% |
| ! | CTTCCAAAGCAAAGTATTCT+TGG | - | chr8.1:75304842-75304861 | None:intergenic | 35.0% |
| ! | GTATTCTTGGTGATTGTTCA+TGG | - | chr8.1:75304829-75304848 | None:intergenic | 35.0% |
| ! | TGATGATGATGCTAGAAAAG+AGG | + | chr8.1:75305007-75305026 | MS.gene060513:CDS | 35.0% |
| ! | TTTGGAACCTAAGTTGCAAA+TGG | - | chr8.1:75304572-75304591 | None:intergenic | 35.0% |
| !! | AAACCATTGAGAGTGTTGTT+AGG | - | chr8.1:75304521-75304540 | None:intergenic | 35.0% |
| AACCACATGAATGAAGGCAA+TGG | + | chr8.1:75305711-75305730 | MS.gene060513:CDS | 40.0% | |
| ACTTGACTCAACCAAGACTT+TGG | - | chr8.1:75305201-75305220 | None:intergenic | 40.0% | |
| ATCTACAAAAGAGTCCTCAC+TGG | - | chr8.1:75305533-75305552 | None:intergenic | 40.0% | |
| CATCCTAACAACACTCTCAA+TGG | + | chr8.1:75304515-75304534 | MS.gene060513:CDS | 40.0% | |
| CTACATCATGCACTTCCTAA+AGG | + | chr8.1:75305333-75305352 | MS.gene060513:intron | 40.0% | |
| TCTTGACATCAAGATCTGGA+TGG | - | chr8.1:75305662-75305681 | None:intergenic | 40.0% | |
| TTATTCTGCAGCCAAAGTCT+TGG | + | chr8.1:75305187-75305206 | MS.gene060513:CDS | 40.0% | |
| TTTCCAATCACCGAGCATAT+CGG | - | chr8.1:75304760-75304779 | None:intergenic | 40.0% | |
| ! | CGTGCATTTTCCTTAACAAG+GGG | - | chr8.1:75305278-75305297 | None:intergenic | 40.0% |
| ! | GACGTGCATTTTCCTTAACA+AGG | - | chr8.1:75305280-75305299 | None:intergenic | 40.0% |
| ! | GTGAGGACTCTTTTGTAGAT+AGG | + | chr8.1:75305533-75305552 | MS.gene060513:intron | 40.0% |
| !! | ATCATAGCCAAGAGTACCAA+TGG | - | chr8.1:75304796-75304815 | None:intergenic | 40.0% |
| AGCCATTGCCTTCATTCATG+TGG | - | chr8.1:75305716-75305735 | None:intergenic | 45.0% | |
| CTTCGCTCCATTTGCAACTT+AGG | + | chr8.1:75304562-75304581 | MS.gene060513:intron | 45.0% | |
| GCTTCATAGTTTGACCAGTG+AGG | + | chr8.1:75305516-75305535 | MS.gene060513:intron | 45.0% | |
| TCTTCAATTGGGTGCACAAG+AGG | + | chr8.1:75304489-75304508 | MS.gene060513:CDS | 45.0% | |
| TGAATGAAGGCAATGGCTCA+AGG | + | chr8.1:75305718-75305737 | MS.gene060513:CDS | 45.0% | |
| ! | ATGCTCGGTGATTGGAAAGA+CGG | + | chr8.1:75304762-75304781 | MS.gene060513:CDS | 45.0% |
| ! | GTTGCTTTGACCGATATGCT+CGG | + | chr8.1:75304747-75304766 | MS.gene060513:CDS | 45.0% |
| ATGGCTGTGTTCTGTGTGCA+TGG | - | chr8.1:75304338-75304357 | None:intergenic | 50.0% | |
| ! | TGACCGATATGCTCGGTGAT+TGG | + | chr8.1:75304754-75304773 | MS.gene060513:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 75304319 | 75305758 | 75304319 | ID=MS.gene060513 |
| chr8.1 | mRNA | 75304319 | 75305758 | 75304319 | ID=MS.gene060513.t1;Parent=MS.gene060513 |
| chr8.1 | exon | 75304319 | 75304324 | 75304319 | ID=MS.gene060513.t1.exon1;Parent=MS.gene060513.t1 |
| chr8.1 | CDS | 75304319 | 75304324 | 75304319 | ID=cds.MS.gene060513.t1;Parent=MS.gene060513.t1 |
| chr8.1 | exon | 75304488 | 75304536 | 75304488 | ID=MS.gene060513.t1.exon2;Parent=MS.gene060513.t1 |
| chr8.1 | CDS | 75304488 | 75304536 | 75304488 | ID=cds.MS.gene060513.t1;Parent=MS.gene060513.t1 |
| chr8.1 | exon | 75304673 | 75305319 | 75304673 | ID=MS.gene060513.t1.exon3;Parent=MS.gene060513.t1 |
| chr8.1 | CDS | 75304673 | 75305319 | 75304673 | ID=cds.MS.gene060513.t1;Parent=MS.gene060513.t1 |
| chr8.1 | exon | 75305630 | 75305758 | 75305630 | ID=MS.gene060513.t1.exon4;Parent=MS.gene060513.t1 |
| chr8.1 | CDS | 75305630 | 75305758 | 75305630 | ID=cds.MS.gene060513.t1;Parent=MS.gene060513.t1 |
| Gene Sequence |
| Protein sequence |
