AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20986

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20986.t1 MTR_1g084820 94.787 633 33 0 1 633 1 633 0.0 1016
MS.gene20986.t1 MTR_1g084820 94.396 571 32 0 1 571 1 571 0.0 896
MS.gene20986.t1 MTR_7g111600 63.402 194 50 8 318 503 64 244 4.82e-56 192
MS.gene20986.t1 MTR_7g111600 63.402 194 50 8 318 503 64 244 8.79e-56 190
MS.gene20986.t1 MTR_7g111600 63.402 194 50 8 318 503 64 244 1.93e-55 191
MS.gene20986.t1 MTR_3g048280 47.468 158 60 4 346 502 60 195 8.37e-36 134
MS.gene20986.t1 MTR_5g091650 53.636 110 50 1 402 510 90 199 3.85e-31 123
MS.gene20986.t1 MTR_5g091650 56.383 94 40 1 413 505 101 194 1.21e-29 116
MS.gene20986.t1 MTR_2g049670 41.589 214 82 6 331 511 46 249 1.93e-29 119
MS.gene20986.t1 MTR_2g049670 48.031 127 49 2 420 546 158 267 3.54e-25 107
MS.gene20986.t1 MTR_8g089400 53.636 110 47 3 405 512 24 131 1.48e-28 112
MS.gene20986.t1 MTR_8g089400 53.636 110 47 3 405 512 44 151 1.95e-28 112
MS.gene20986.t1 MTR_1g056640 57.609 92 37 2 416 505 402 493 1.96e-27 116
MS.gene20986.t1 MTR_8g085390 45.038 131 60 2 419 548 29 148 6.60e-27 108
MS.gene20986.t1 MTR_4g132280 50.485 103 49 2 412 512 374 476 4.46e-26 112
MS.gene20986.t1 MTR_5g015720 26.984 441 205 16 87 502 66 414 1.21e-25 110
MS.gene20986.t1 MTR_8g085440 51.685 89 42 1 416 503 28 116 2.55e-25 101
MS.gene20986.t1 MTR_8g088120 62.821 78 28 1 429 505 57 134 2.70e-25 105
MS.gene20986.t1 MTR_8g085400 59.494 79 30 1 418 496 31 107 3.21e-24 98.2
MS.gene20986.t1 MTR_5g085580 52.128 94 43 2 414 505 376 469 3.29e-22 100
MS.gene20986.t1 MTR_8g085420 48.810 84 42 1 416 498 28 111 9.77e-22 92.0
MS.gene20986.t1 MTR_3g083580 46.000 100 52 2 418 515 360 459 1.10e-21 99.4
MS.gene20986.t1 MTR_5g097420 51.613 93 43 2 412 502 22 114 1.34e-21 90.9
MS.gene20986.t1 MTR_8g085720 47.368 95 48 2 420 512 364 458 1.30e-20 95.9
MS.gene20986.t1 MTR_5g078200 47.917 96 48 2 412 505 352 447 1.62e-20 96.3
MS.gene20986.t1 MTR_5g078200 47.917 96 48 2 412 505 319 414 1.69e-20 96.3
MS.gene20986.t1 MTR_6g088885 38.739 111 65 2 408 515 357 467 2.05e-20 95.5
MS.gene20986.t1 MTR_3g082080 53.846 78 35 1 420 496 157 234 2.36e-20 91.3
MS.gene20986.t1 MTR_4g083500 41.000 100 58 1 418 516 400 499 2.80e-20 95.1
MS.gene20986.t1 MTR_1g022160 47.778 90 46 1 419 507 390 479 3.46e-20 94.4
MS.gene20986.t1 MTR_4g079530 44.554 101 54 2 410 508 27 127 7.38e-20 86.3
MS.gene20986.t1 MTR_3g065460 40.984 122 70 2 397 516 338 459 1.41e-19 92.8
MS.gene20986.t1 MTR_4g109490 52.941 85 38 2 419 502 23 106 1.48e-19 88.2
MS.gene20986.t1 MTR_1g116870 45.455 88 47 1 416 502 272 359 1.57e-19 91.3
MS.gene20986.t1 MTR_4g133735 48.352 91 45 2 414 502 22 112 1.62e-19 84.7
MS.gene20986.t1 MTR_4g079530 49.474 95 46 2 420 512 37 131 1.65e-19 87.0
MS.gene20986.t1 MTR_5g044530 50.000 92 43 2 417 505 19 110 1.75e-19 89.0
MS.gene20986.t1 MTR_4g083070 50.000 88 42 2 420 505 21 108 5.72e-19 85.9
MS.gene20986.t1 MTR_8g102340 36.441 118 73 2 411 526 325 442 1.47e-18 89.4
MS.gene20986.t1 MTR_1g022160 48.235 85 43 1 419 502 390 474 1.71e-18 89.4
MS.gene20986.t1 MTR_4g069940 45.133 113 53 4 403 507 353 464 1.88e-18 89.0
MS.gene20986.t1 MTR_4g133730 46.154 91 47 2 414 502 22 112 2.10e-18 81.6
MS.gene20986.t1 MTR_1g031900 40.517 116 67 2 418 531 359 474 4.42e-18 88.2
MS.gene20986.t1 MTR_2g072500 47.674 86 43 2 418 502 6 90 6.21e-18 79.7
MS.gene20986.t1 MTR_1g031900 48.276 87 43 2 418 502 359 445 1.10e-17 87.0
MS.gene20986.t1 MTR_1g022160 47.500 80 41 1 419 497 390 469 4.05e-17 85.1
MS.gene20986.t1 MTR_8g012400 39.216 102 55 3 405 504 284 380 2.49e-16 81.6
MS.gene20986.t1 MTR_8g012400 39.216 102 55 3 405 504 364 460 2.58e-16 82.4
MS.gene20986.t1 MTR_7g053500 42.478 113 60 5 395 504 273 383 4.39e-16 80.9
MS.gene20986.t1 MTR_3g116510 42.202 109 58 3 403 507 218 325 7.79e-16 79.3
MS.gene20986.t1 MTR_7g053500 42.478 113 60 5 395 504 350 460 8.34e-16 80.9
MS.gene20986.t1 MTR_3g116510 42.202 109 58 3 403 507 352 459 9.78e-16 80.5
MS.gene20986.t1 MTR_7g026340 38.235 102 62 1 400 500 345 446 6.47e-14 74.7
MS.gene20986.t1 MTR_4g088155 39.623 106 57 4 402 504 358 459 1.90e-13 73.6
MS.gene20986.t1 MTR_3g031770 31.333 150 87 5 400 535 342 489 1.71e-12 70.5
MS.gene20986.t1 MTR_1g053320 35.849 106 58 5 418 518 372 472 8.97e-12 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20986.t1 AT1G09460 69.048 126 36 2 386 509 101 225 9.99e-44 160
MS.gene20986.t1 AT2G30933 59.574 94 37 1 418 510 80 173 5.03e-33 127
MS.gene20986.t1 AT1G29380 64.444 90 31 1 420 508 148 237 1.65e-31 124
MS.gene20986.t1 AT1G29380 65.476 84 28 1 420 502 148 231 3.38e-30 121
MS.gene20986.t1 AT2G30933 43.846 130 41 2 368 496 61 159 8.81e-29 112
MS.gene20986.t1 AT2G30933 43.846 130 41 2 368 496 61 159 8.81e-29 112
MS.gene20986.t1 AT1G29380 66.667 78 25 1 420 496 148 225 2.18e-28 114
MS.gene20986.t1 AT1G29380 66.667 78 25 1 420 496 148 225 2.18e-28 114
MS.gene20986.t1 AT2G05790 53.448 116 49 3 395 505 356 471 3.50e-28 119
MS.gene20986.t1 AT1G11820 51.515 99 46 2 418 514 379 477 1.79e-25 110
MS.gene20986.t1 AT4G13600 44.094 127 66 3 420 542 61 186 2.13e-25 105
MS.gene20986.t1 AT4G05430 44.444 117 63 2 400 514 3 119 2.65e-25 102
MS.gene20986.t1 AT4G05430 44.167 120 65 2 397 514 25 144 6.99e-25 102
MS.gene20986.t1 AT5G55180 52.632 95 43 2 413 505 365 459 1.73e-24 107
MS.gene20986.t1 AT4G26830 35.751 193 110 6 322 505 267 454 1.19e-23 104
MS.gene20986.t1 AT3G55430 46.739 92 48 1 412 502 358 449 1.34e-23 104
MS.gene20986.t1 AT4G26830 35.751 193 110 6 322 505 271 458 1.42e-23 104
MS.gene20986.t1 AT5G55180 55.814 86 36 2 413 496 365 450 2.90e-22 100
MS.gene20986.t1 AT5G35740 52.747 91 40 3 415 502 25 115 3.14e-22 92.8
MS.gene20986.t1 AT4G34480 47.674 86 44 1 419 503 252 337 1.72e-21 97.4
MS.gene20986.t1 AT2G01630 39.535 129 74 3 418 544 358 484 1.78e-21 98.6
MS.gene20986.t1 AT1G18650 47.778 90 46 1 419 507 20 109 2.36e-21 92.4
MS.gene20986.t1 AT4G34480 47.674 86 44 1 419 503 363 448 2.91e-21 98.2
MS.gene20986.t1 AT3G58100 47.872 94 47 2 420 511 41 134 5.39e-21 91.3
MS.gene20986.t1 AT1G11820 54.321 81 35 2 418 496 379 459 7.44e-21 96.7
MS.gene20986.t1 AT3G58100 46.939 98 50 2 416 511 113 210 9.26e-21 92.8
MS.gene20986.t1 AT1G18650 47.191 89 46 1 419 506 20 108 1.22e-20 88.6
MS.gene20986.t1 AT4G05430 46.078 102 53 2 397 496 25 126 1.43e-20 88.2
MS.gene20986.t1 AT1G79480 50.000 84 41 1 420 502 310 393 3.90e-20 93.6
MS.gene20986.t1 AT1G79480 50.000 84 41 1 420 502 311 394 3.98e-20 93.6
MS.gene20986.t1 AT1G66250 39.474 114 66 3 418 529 366 478 6.10e-20 94.0
MS.gene20986.t1 AT2G16230 39.806 103 61 1 419 520 365 467 1.17e-19 93.2
MS.gene20986.t1 AT5G61130 47.674 86 44 1 419 503 20 105 1.39e-19 87.8
MS.gene20986.t1 AT1G69295 56.338 71 29 2 419 488 20 89 2.78e-19 87.4
MS.gene20986.t1 AT1G69295 56.338 71 29 2 419 488 16 85 4.61e-19 86.7
MS.gene20986.t1 AT5G67460 48.235 85 42 2 420 502 293 377 4.63e-19 90.1
MS.gene20986.t1 AT1G26450 38.312 154 67 6 421 550 22 171 1.08e-18 85.1
MS.gene20986.t1 AT5G56590 40.777 103 59 2 420 520 369 471 1.22e-18 90.1
MS.gene20986.t1 AT5G63225 46.512 86 45 1 418 502 23 108 1.39e-18 82.0
MS.gene20986.t1 AT1G66870 42.529 87 48 2 418 502 51 137 2.83e-18 82.0
MS.gene20986.t1 AT1G66870 42.529 87 48 2 418 502 24 110 2.96e-18 81.3
MS.gene20986.t1 AT5G67460 50.633 79 37 2 420 496 293 371 3.19e-18 87.4
MS.gene20986.t1 AT4G29360 45.977 87 45 2 418 502 399 485 3.37e-18 89.0
MS.gene20986.t1 AT4G29360 45.977 87 45 2 418 502 389 475 3.65e-18 88.6
MS.gene20986.t1 AT4G09462 41.758 91 52 1 415 504 24 114 4.27e-18 80.9
MS.gene20986.t1 AT5G24318 36.697 109 62 2 398 505 217 319 5.33e-18 85.9
MS.gene20986.t1 AT5G24318 36.697 109 62 2 398 505 356 458 6.27e-18 87.4
MS.gene20986.t1 AT4G09465 40.659 91 53 1 415 504 26 116 8.07e-18 80.1
MS.gene20986.t1 AT4G09467 40.659 91 53 1 415 504 26 116 1.01e-17 79.7
MS.gene20986.t1 AT4G09464 40.659 91 53 1 415 504 26 116 1.01e-17 79.7
MS.gene20986.t1 AT5G08000 44.262 122 66 2 417 536 37 158 1.09e-17 82.8
MS.gene20986.t1 AT3G13560 43.434 99 54 2 420 516 362 460 1.22e-17 86.7
MS.gene20986.t1 AT3G13560 43.434 99 54 2 420 516 362 460 1.22e-17 86.7
MS.gene20986.t1 AT3G13560 43.434 99 54 2 420 516 362 460 1.22e-17 86.7
MS.gene20986.t1 AT3G13560 43.434 99 54 2 420 516 362 460 1.22e-17 86.7
MS.gene20986.t1 AT3G13560 43.434 99 54 2 420 516 362 460 1.22e-17 86.7
MS.gene20986.t1 AT4G09466 40.659 91 53 1 415 504 26 116 1.99e-17 79.0
MS.gene20986.t1 AT4G09090 43.023 86 48 1 420 504 31 116 3.11e-17 78.6
MS.gene20986.t1 AT2G42930 44.872 78 43 0 420 497 40 117 3.23e-17 79.0
MS.gene20986.t1 AT2G01630 48.148 81 40 2 418 496 358 438 3.98e-17 84.7
MS.gene20986.t1 AT1G66852 39.535 86 51 1 418 502 24 109 5.17e-17 77.8
MS.gene20986.t1 AT5G08000 47.674 86 44 1 419 503 20 105 9.83e-17 79.3
MS.gene20986.t1 AT3G23770 44.118 102 53 4 405 504 374 473 1.06e-16 83.6
MS.gene20986.t1 AT1G78520 40.000 90 52 2 414 502 26 114 1.94e-16 76.3
MS.gene20986.t1 AT2G04910 43.182 88 48 2 415 500 10 97 2.08e-16 75.9
MS.gene20986.t1 AT5G63240 36.275 102 61 2 407 504 27 128 6.67e-16 75.1
MS.gene20986.t1 AT5G24318 37.000 100 56 2 398 496 356 449 1.52e-15 80.1
MS.gene20986.t1 AT5G63250 38.776 98 55 3 412 504 33 130 3.08e-15 73.2
MS.gene20986.t1 AT5G63230 37.363 91 56 1 413 502 19 109 3.78e-15 72.4
MS.gene20986.t1 AT1G66855 37.931 87 52 2 418 502 24 110 6.21e-15 71.6
MS.gene20986.t1 AT4G16165 37.931 87 52 2 418 502 24 110 2.05e-14 70.1
MS.gene20986.t1 AT2G43670 38.298 94 55 3 410 502 26 117 4.17e-14 69.7
MS.gene20986.t1 AT5G53600 39.535 86 50 2 418 502 27 111 4.97e-14 69.3
MS.gene20986.t1 AT2G04910 42.857 84 46 2 415 496 10 93 7.54e-14 68.9
MS.gene20986.t1 AT2G39640 34.091 88 57 1 419 505 459 546 8.27e-14 75.1
MS.gene20986.t1 AT2G39640 35.417 96 55 2 406 500 351 440 3.25e-12 69.7
MS.gene20986.t1 AT2G43660 41.667 96 52 4 409 502 26 119 1.26e-13 68.6
MS.gene20986.t1 AT2G43660 41.667 96 52 4 409 502 25 118 1.80e-13 67.8
MS.gene20986.t1 AT3G07320 39.216 102 58 3 405 504 358 457 3.94e-13 72.4
MS.gene20986.t1 AT2G29735 39.785 93 53 2 413 502 24 116 5.81e-13 66.2
MS.gene20986.t1 AT4G14080 37.255 102 62 2 405 504 374 475 8.09e-13 71.6
MS.gene20986.t1 AT1G13830 39.130 92 47 3 417 502 32 120 8.95e-13 68.2
MS.gene20986.t1 AT1G13830 39.326 89 45 3 420 502 21 106 1.23e-12 67.8
MS.gene20986.t1 AT2G03505 36.667 90 54 2 419 505 20 109 1.48e-12 65.1
MS.gene20986.t1 AT2G03505 36.667 90 54 2 419 505 20 109 1.77e-12 66.2
MS.gene20986.t1 AT2G03505 36.667 90 54 2 419 505 20 109 1.77e-12 66.2
MS.gene20986.t1 AT2G03505 36.667 90 54 2 419 505 20 109 1.77e-12 66.2
MS.gene20986.t1 AT5G53610 41.463 82 46 2 420 500 28 108 2.10e-12 64.3
MS.gene20986.t1 AT5G64790 38.144 97 56 4 418 512 367 461 7.66e-12 68.6

Find 141 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGGTACAATGCCAGATTT+TGG 0.194586 1.3:+60145273 MS.gene20986:CDS
CCAACATCTTCAGGAACTTT+TGG 0.225114 1.3:+60145103 MS.gene20986:CDS
TCACTTTCATGCAGGTTCTT+TGG 0.248475 1.3:+60143378 MS.gene20986:intron
TCTCTCTTAATTTCATGTTC+TGG 0.269637 1.3:+60143282 MS.gene20986:CDS
TAGATTACTTTCATTTATAA+AGG 0.277901 1.3:+60143834 MS.gene20986:CDS
GGTGGAGCTGTTGGAATTGA+TGG 0.308099 1.3:-60145082 None:intergenic
AAGACTATACAGCCAGTAAA+AGG 0.355621 1.3:-60145354 None:intergenic
GGAGGCGCTGCTATGTTAGT+TGG 0.360601 1.3:+60144783 MS.gene20986:CDS
GAGCTTTCGTCACGGATCAT+AGG 0.364800 1.3:+60143479 MS.gene20986:CDS
GGAGGTGGATAAGATGTTAC+TGG 0.377508 1.3:-60144486 None:intergenic
ATGATAGAAGATAGCATTGA+TGG 0.377602 1.3:+60143874 MS.gene20986:CDS
CCTCCAAAGTCGCAACTTGT+TGG 0.380624 1.3:-60144765 None:intergenic
GGTGTAATGGTTGGAGTTGT+TGG 0.382964 1.3:-60144390 None:intergenic
GGTGGAGGTGTTGTTAGATT+TGG 0.383669 1.3:-60145016 None:intergenic
GGCATTGCAGCTGGATTTGT+AGG 0.384777 1.3:-60144327 None:intergenic
TGTGGAAGGAACTGCTATAA+TGG 0.388341 1.3:-60144286 None:intergenic
AACTCAAGCAACCCAGCTTC+AGG 0.391337 1.3:+60145255 MS.gene20986:CDS
GCTCCAACAAGTTGCGACTT+TGG 0.392668 1.3:+60144762 MS.gene20986:CDS
ATAACTGGTGTTGGATTTGT+AGG 0.394170 1.3:-60144243 None:intergenic
GCATGGTTCTGCAAAGTAAC+AGG 0.394899 1.3:-60144702 None:intergenic
ACGAAGAAACTTTGCTCTGC+TGG 0.394901 1.3:-60143447 None:intergenic
GGAGGTGTTGTTAGATTTGG+TGG 0.400113 1.3:-60145013 None:intergenic
CGAGTTACTTCTATTGAACT+TGG 0.401043 1.3:-60144015 None:intergenic
TTGGATTATGCTTGTGGAAA+TGG 0.401736 1.3:+60144636 MS.gene20986:CDS
ACAGCTCCACCAACATCTTC+AGG 0.402649 1.3:+60145094 MS.gene20986:CDS
CTTCAATGAAAGAACTCAAA+AGG 0.413209 1.3:+60144118 MS.gene20986:CDS
GTAGTTGAAGGAACTGTTAC+TGG 0.413784 1.3:-60144348 None:intergenic
GAAGGAGCATTTGTGTTTGA+AGG 0.413852 1.3:-60144549 None:intergenic
GTTGGAGTTGTTGGAGGTAA+AGG 0.413921 1.3:-60144381 None:intergenic
TTGAAGGAGGTGGCTGTTGA+GGG 0.417203 1.3:-60144533 None:intergenic
CACAAATGCTCCTTCTGCTC+AGG 0.420750 1.3:+60144557 MS.gene20986:CDS
ACCACTGGAACATTTCCTAA+TGG 0.424925 1.3:-60144507 None:intergenic
GGATAAGATGTTACTGGAGG+TGG 0.426667 1.3:-60144480 None:intergenic
GCCGATGTTGTGTTTACAAT+AGG 0.430103 1.3:-60145309 None:intergenic
ACTGTTACTGGCATTGCAGC+TGG 0.430772 1.3:-60144336 None:intergenic
TGATTGTTCTCAAATACAAC+AGG 0.433295 1.3:+60144662 MS.gene20986:CDS
ACAACTCCTACAACAGTTCC+AGG 0.436358 1.3:+60144435 MS.gene20986:CDS
ACATCTTATCCACCTCCATT+AGG 0.436623 1.3:+60144492 MS.gene20986:CDS
CTTTCATGCAGGTTCTTTGG+TGG 0.439102 1.3:+60143381 MS.gene20986:intron
GGAAATTGTGACAGGATTTG+TGG 0.439347 1.3:-60144304 None:intergenic
GGAGCTATTGTTGGAATAGA+TGG 0.441593 1.3:-60145037 None:intergenic
GGGCTATCAGACCCAAAATC+TGG 0.444861 1.3:-60145285 None:intergenic
TTTCATGCAGGTTCTTTGGT+GGG 0.448012 1.3:+60143382 MS.gene20986:intron
TTTGAAGGAGGTGGCTGTTG+AGG 0.448619 1.3:-60144534 None:intergenic
GGTGGAGCTGTTGACATTGT+TGG 0.450114 1.3:-60145061 None:intergenic
AGCTTCCCTTCAATCAGCAT+TGG 0.454325 1.3:+60144617 MS.gene20986:CDS
AACTTCCTCAAGACTTGAAG+TGG 0.458266 1.3:-60143784 None:intergenic
CTGAGGCTTGCAGTGACAAA+TGG 0.467298 1.3:-60145384 None:intergenic
ATTGTTGGTGGAGCTATTGT+TGG 0.472956 1.3:-60145046 None:intergenic
AGTTGGTGTGTGGCGAAAAC+AGG 0.475765 1.3:+60144585 MS.gene20986:CDS
AAGTCGCAACTTGTTGGAGC+TGG 0.478203 1.3:-60144759 None:intergenic
GAGGCAGAAAGAAGCTTCCT+TGG 0.478234 1.3:-60143247 None:intergenic
TCTGGCATTGTACCTGAAGC+TGG 0.478959 1.3:-60145267 None:intergenic
GGTGCACCTGGAACTGTTGT+AGG 0.485248 1.3:-60144441 None:intergenic
GTAGGGTAAACTGTGTTTGT+TGG 0.486254 1.3:-60144225 None:intergenic
GGAGGTGGATAAGATGTTAC+TGG 0.487201 1.3:-60144465 None:intergenic
GGCTGTTGAGGGTTTACCAC+TGG 0.491135 1.3:-60144522 None:intergenic
CATAATCCAATGCTGATTGA+AGG 0.493090 1.3:-60144623 None:intergenic
GTTGATTGTGTAGGTGGTGT+TGG 0.494210 1.3:-60144974 None:intergenic
TTTGTAGGGGAAATTGTGAC+AGG 0.494725 1.3:-60144312 None:intergenic
TATCTAGAATATTTCTTCTG+TGG 0.496929 1.3:-60144191 None:intergenic
AGTGTCTCCTCTCTCATGAT+AGG 0.500543 1.3:-60143410 None:intergenic
GTATCTGAGGGTGTAATAAC+TGG 0.502389 1.3:-60144258 None:intergenic
GCATTGCAGCTGGATTTGTA+GGG 0.503176 1.3:-60144326 None:intergenic
GATGTTACTGGTGGTGCACC+TGG 0.511282 1.3:-60144453 None:intergenic
TTCAATGAAAGAACTCAAAA+GGG 0.511868 1.3:+60144119 MS.gene20986:CDS
CTTGAGTTTAACACTGAAGG+TGG 0.514620 1.3:-60145240 None:intergenic
TCAGCATTGGATTATGCTTG+TGG 0.514748 1.3:+60144630 MS.gene20986:CDS
GCATTTGTGTTTGAAGGAGG+TGG 0.517825 1.3:-60144543 None:intergenic
CTCTTGATTGTAAGAACCAC+AGG 0.520516 1.3:-60143982 None:intergenic
CATTTCCACTTCAAGTCTTG+AGG 0.520651 1.3:+60143779 MS.gene20986:CDS
GGAGCATTTGTGTTTGAAGG+AGG 0.521515 1.3:-60144546 None:intergenic
GAAGACTGGTGCCGGTGTAA+TGG 0.528319 1.3:-60144403 None:intergenic
GATGTTGTGTTTACAATAGG+AGG 0.530245 1.3:-60145306 None:intergenic
CAACTCTGTCCCTGAGCAGA+AGG 0.531643 1.3:-60144567 None:intergenic
TCCTTGCAAAGATGTTCCTG+TGG 0.532621 1.3:+60143966 MS.gene20986:CDS
CCAACAAGTTGCGACTTTGG+AGG 0.533263 1.3:+60144765 MS.gene20986:CDS
GGAACAGCAGGTGTAGTTGA+AGG 0.536488 1.3:-60144360 None:intergenic
GAAGATGTTGGTGGAGCTGT+TGG 0.537146 1.3:-60145091 None:intergenic
GAAAGAACTCAAAAGGGAAG+AGG 0.546037 1.3:+60144125 MS.gene20986:CDS
GGAACATTTCCTAATGGAGG+TGG 0.552963 1.3:-60144501 None:intergenic
CCAAAAGTTCCTGAAGATGT+TGG 0.553073 1.3:-60145103 None:intergenic
GTTGGAGGTAAAGGAACAGC+AGG 0.553488 1.3:-60144372 None:intergenic
GGAGCTGTTGACATTGTTGG+TGG 0.559801 1.3:-60145058 None:intergenic
GAGGGTGTAATAACTGGTGT+TGG 0.563477 1.3:-60144252 None:intergenic
ATAATCCAATGCTGATTGAA+GGG 0.564193 1.3:-60144622 None:intergenic
ACTGTTGTAGGAGTTGTAGC+TGG 0.566759 1.3:-60144429 None:intergenic
TTTAGCTCAGAAACTGCTGA+AGG 0.570280 1.3:-60143586 None:intergenic
GGTGTTGTTAGATTTGGTGG+TGG 0.572595 1.3:-60145010 None:intergenic
TTGAGTTTAACACTGAAGGT+GGG 0.573230 1.3:-60145239 None:intergenic
AAAAGCATCGTTGCTAGCTG+TGG 0.575060 1.3:+60143646 MS.gene20986:CDS
TAACTGGTGTTGGATTTGTA+GGG 0.580532 1.3:-60144242 None:intergenic
CTTCTGCTCAGGGACAGAGT+TGG 0.581714 1.3:+60144568 MS.gene20986:CDS
TTGCTTGAGTTTAACACTGA+AGG 0.581994 1.3:-60145243 None:intergenic
AAAGTTCCTGAAGATGTTGG+TGG 0.585173 1.3:-60145100 None:intergenic
TTAAGAGCATGCATAATCAA+AGG 0.588835 1.3:-60143697 None:intergenic
TTTCAGAGCTCGAGGGTGCG+AGG 0.590599 1.3:-60145331 None:intergenic
TATAATGGTTGGAGTATCTG+AGG 0.591925 1.3:-60144271 None:intergenic
GAAGGAACTGCTATAATGGT+TGG 0.591976 1.3:-60144282 None:intergenic
AAGATCTCTCTTCTAGACAA+AGG 0.594620 1.3:-60144159 None:intergenic
GGTGTTGGAGATGATATCAT+TGG 0.598845 1.3:-60144959 None:intergenic
ATACTCTCTGATAATGGCCA+AGG 0.605015 1.3:+60143230 None:intergenic
GTAATGGTTGGAGTTGTTGG+AGG 0.606112 1.3:-60144387 None:intergenic
ATCATGAGAGAGGAGACACT+TGG 0.607322 1.3:+60143413 MS.gene20986:CDS
GTAAAAGGTTTCAGAGCTCG+AGG 0.607544 1.3:-60145339 None:intergenic
TGAGTTTAACACTGAAGGTG+GGG 0.608299 1.3:-60145238 None:intergenic
GAAGTTGAAGTTGATTGTGT+AGG 0.609053 1.3:-60144983 None:intergenic
TGATATCATTGGTGAAGTTG+AGG 0.609455 1.3:-60144948 None:intergenic
GTTGAAGTTGATTGTGTAGG+TGG 0.611559 1.3:-60144980 None:intergenic
ATGTTGTGTTTACAATAGGA+GGG 0.618672 1.3:-60145305 None:intergenic
GTTGTAGCTGGATTGAAGAC+TGG 0.620005 1.3:-60144417 None:intergenic
ACAAATGCTCCTTCTGCTCA+GGG 0.620260 1.3:+60144558 MS.gene20986:CDS
ATTGTGACAGGATTTGTGGA+AGG 0.620979 1.3:-60144300 None:intergenic
AGAGAGAAGAGAGAAACAAG+AGG 0.623621 1.3:-60143266 None:intergenic
GCTGGATTGAAGACTGGTGC+CGG 0.624496 1.3:-60144411 None:intergenic
AACAACTCCAACCATTACAC+CGG 0.624559 1.3:+60144392 MS.gene20986:CDS
GGAGCTGTTGGAATTGATGG+TGG 0.624833 1.3:-60145079 None:intergenic
ACTGGTGCCGGTGTAATGGT+TGG 0.625957 1.3:-60144399 None:intergenic
CTGGCATTGTACCTGAAGCT+GGG 0.626526 1.3:-60145266 None:intergenic
CAGTGATCTTCACATAAGCA+AGG 0.629071 1.3:+60143738 MS.gene20986:CDS
TTAGCTCAGAAACTGCTGAA+GGG 0.631188 1.3:-60143585 None:intergenic
ACCACAGGAACATCTTTGCA+AGG 0.631919 1.3:-60143967 None:intergenic
AACTCTATAGGTGCATGCTG+AGG 0.632489 1.3:-60145401 None:intergenic
CATTGCAGCTGGATTTGTAG+GGG 0.635434 1.3:-60144325 None:intergenic
GAACTCAAAAGGGAAGAGGA+AGG 0.639773 1.3:+60144129 MS.gene20986:CDS
TGTTGAAGGCAAAAGAAGCA+TGG 0.645483 1.3:-60144719 None:intergenic
GCTATTGTTGGAATAGATGG+TGG 0.647261 1.3:-60145034 None:intergenic
TCCATTAGGAAATGTTCCAG+TGG 0.658091 1.3:+60144506 MS.gene20986:CDS
ACTGGAACATTTCCTAATGG+AGG 0.658231 1.3:-60144504 None:intergenic
TCAGATTCGAGCTTTCGTCA+CGG 0.659259 1.3:+60143471 MS.gene20986:CDS
GCTGATTGAAGGGAAGCTTG+TGG 0.661755 1.3:-60144612 None:intergenic
TAAAAGGTTTCAGAGCTCGA+GGG 0.668012 1.3:-60145338 None:intergenic
ATTGTTGGAATAGATGGTGG+AGG 0.673696 1.3:-60145031 None:intergenic
TCCTATTGTAAACACAACAT+CGG 0.678848 1.3:+60145308 MS.gene20986:CDS
CAGAAGCAGCAAGATTAGCG+CGG 0.682382 1.3:-60143939 None:intergenic
ATAATGGTTGGAGTATCTGA+GGG 0.698374 1.3:-60144270 None:intergenic
TCAGGGACAGAGTTGGTGTG+TGG 0.715688 1.3:+60144575 MS.gene20986:CDS
GGTGGATAAGATGTTACTGG+AGG 0.717889 1.3:-60144483 None:intergenic
GATTGTTCTCAAATACAACA+GGG 0.719905 1.3:+60144663 MS.gene20986:CDS
GACGACGATGAAGATGATGA+CGG 0.723295 1.3:-60144920 None:intergenic
GGTGGATAAGATGTTACTGG+TGG 0.740803 1.3:-60144462 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGATTACTTTCATTTATAA+AGG + chr1.3:60143834-60143853 MS.gene20986:CDS 15.0%
!! TTAAATGTTAAAATCTTACT+TGG - chr1.3:60144816-60144835 None:intergenic 15.0%
!!! AAATAATGAATTTTTCAACT+TGG - chr1.3:60143561-60143580 None:intergenic 15.0%
!!! TATTGATGATCATTTTTAAT+AGG + chr1.3:60144878-60144897 MS.gene20986:intron 15.0%
!! ATTGAAAGAAAGAAAAAACA+AGG - chr1.3:60144844-60144863 None:intergenic 20.0%
!! TTGAAAGAAAGAAAAAACAA+GGG - chr1.3:60144843-60144862 None:intergenic 20.0%
!!! TCTTTTTGCTTCTAAATATT+TGG - chr1.3:60144058-60144077 None:intergenic 20.0%
! ATATTATTTCACTTTCATGC+AGG + chr1.3:60143370-60143389 MS.gene20986:intron 25.0%
! TTCAATGAAAGAACTCAAAA+GGG + chr1.3:60144119-60144138 MS.gene20986:CDS 25.0%
!! ATGCTTTTGATGTTTAAATG+TGG - chr1.3:60143634-60143653 None:intergenic 25.0%
!! TATCTAGAATATTTCTTCTG+TGG - chr1.3:60144194-60144213 None:intergenic 25.0%
!!! TGATCATTTTTAATAGGTTC+TGG + chr1.3:60144884-60144903 MS.gene20986:intron 25.0%
AAGCAAGGAAGTAAAAATCT+CGG + chr1.3:60143753-60143772 MS.gene20986:CDS 30.0%
ATGTTGTGTTTACAATAGGA+GGG - chr1.3:60145308-60145327 None:intergenic 30.0%
CAATAAGCATGTACTAATCA+TGG + chr1.3:60145178-60145197 MS.gene20986:intron 30.0%
CATGATTAGTACATGCTTAT+TGG - chr1.3:60145180-60145199 None:intergenic 30.0%
CTTCAATGAAAGAACTCAAA+AGG + chr1.3:60144118-60144137 MS.gene20986:CDS 30.0%
GATTGTTCTCAAATACAACA+GGG + chr1.3:60144663-60144682 MS.gene20986:CDS 30.0%
TCCTATTGTAAACACAACAT+CGG + chr1.3:60145308-60145327 MS.gene20986:CDS 30.0%
TCTCTCTTAATTTCATGTTC+TGG + chr1.3:60143282-60143301 MS.gene20986:CDS 30.0%
TGATTGTTCTCAAATACAAC+AGG + chr1.3:60144662-60144681 MS.gene20986:CDS 30.0%
! ATAATCCAATGCTGATTGAA+GGG - chr1.3:60144625-60144644 None:intergenic 30.0%
! ATGATAGAAGATAGCATTGA+TGG + chr1.3:60143874-60143893 MS.gene20986:CDS 30.0%
! TTAAGAGCATGCATAATCAA+AGG - chr1.3:60143700-60143719 None:intergenic 30.0%
!! CTTTTGATAGTAACTGTTGA+AGG - chr1.3:60144736-60144755 None:intergenic 30.0%
AAGACTATACAGCCAGTAAA+AGG - chr1.3:60145357-60145376 None:intergenic 35.0%
AAGATCTCTCTTCTAGACAA+AGG - chr1.3:60144162-60144181 None:intergenic 35.0%
CGAGTTACTTCTATTGAACT+TGG - chr1.3:60144018-60144037 None:intergenic 35.0%
GATGTTGTGTTTACAATAGG+AGG - chr1.3:60145309-60145328 None:intergenic 35.0%
TGATATCATTGGTGAAGTTG+AGG - chr1.3:60144951-60144970 None:intergenic 35.0%
TTGAGTTTAACACTGAAGGT+GGG - chr1.3:60145242-60145261 None:intergenic 35.0%
TTGCTTGAGTTTAACACTGA+AGG - chr1.3:60145246-60145265 None:intergenic 35.0%
TTGGATTATGCTTGTGGAAA+TGG + chr1.3:60144636-60144655 MS.gene20986:CDS 35.0%
! AATGCTGTCTTTTGTAGCTA+TGG + chr1.3:60145213-60145232 MS.gene20986:intron 35.0%
! ATAACTGGTGTTGGATTTGT+AGG - chr1.3:60144246-60144265 None:intergenic 35.0%
! ATAATGGTTGGAGTATCTGA+GGG - chr1.3:60144273-60144292 None:intergenic 35.0%
! CAACATCTTCAGGAACTTTT+GGG + chr1.3:60145104-60145123 MS.gene20986:CDS 35.0%
! CATAATCCAATGCTGATTGA+AGG - chr1.3:60144626-60144645 None:intergenic 35.0%
! GAAGTTGAAGTTGATTGTGT+AGG - chr1.3:60144986-60145005 None:intergenic 35.0%
! TAACTGGTGTTGGATTTGTA+GGG - chr1.3:60144245-60144264 None:intergenic 35.0%
! TATAATGGTTGGAGTATCTG+AGG - chr1.3:60144274-60144293 None:intergenic 35.0%
AAAGTTCCTGAAGATGTTGG+TGG - chr1.3:60145103-60145122 None:intergenic 40.0%
AACAACTCCAACCATTACAC+CGG + chr1.3:60144392-60144411 MS.gene20986:CDS 40.0%
AACTTCCTCAAGACTTGAAG+TGG - chr1.3:60143787-60143806 None:intergenic 40.0%
ACATCTTATCCACCTCCATT+AGG + chr1.3:60144492-60144511 MS.gene20986:CDS 40.0%
ACCACTGGAACATTTCCTAA+TGG - chr1.3:60144510-60144529 None:intergenic 40.0%
ACTGGAACATTTCCTAATGG+AGG - chr1.3:60144507-60144526 None:intergenic 40.0%
AGAGAGAAGAGAGAAACAAG+AGG - chr1.3:60143269-60143288 None:intergenic 40.0%
ATTGTGACAGGATTTGTGGA+AGG - chr1.3:60144303-60144322 None:intergenic 40.0%
CAGTGATCTTCACATAAGCA+AGG + chr1.3:60143738-60143757 MS.gene20986:CDS 40.0%
CATTTCCACTTCAAGTCTTG+AGG + chr1.3:60143779-60143798 MS.gene20986:CDS 40.0%
CCAAAAGTTCCTGAAGATGT+TGG - chr1.3:60145106-60145125 None:intergenic 40.0%
CTCTTGATTGTAAGAACCAC+AGG - chr1.3:60143985-60144004 None:intergenic 40.0%
CTTGAGTTTAACACTGAAGG+TGG - chr1.3:60145243-60145262 None:intergenic 40.0%
GAAAGAACTCAAAAGGGAAG+AGG + chr1.3:60144125-60144144 MS.gene20986:CDS 40.0%
GAAGGAACTGCTATAATGGT+TGG - chr1.3:60144285-60144304 None:intergenic 40.0%
GCCGATGTTGTGTTTACAAT+AGG - chr1.3:60145312-60145331 None:intergenic 40.0%
GCTATTGTTGGAATAGATGG+TGG - chr1.3:60145037-60145056 None:intergenic 40.0%
GGAAATTGTGACAGGATTTG+TGG - chr1.3:60144307-60144326 None:intergenic 40.0%
GGAGCTATTGTTGGAATAGA+TGG - chr1.3:60145040-60145059 None:intergenic 40.0%
GTAGGGTAAACTGTGTTTGT+TGG - chr1.3:60144228-60144247 None:intergenic 40.0%
GTAGTTGAAGGAACTGTTAC+TGG - chr1.3:60144351-60144370 None:intergenic 40.0%
TAAAAGGTTTCAGAGCTCGA+GGG - chr1.3:60145341-60145360 None:intergenic 40.0%
TCACTTTCATGCAGGTTCTT+TGG + chr1.3:60143378-60143397 MS.gene20986:intron 40.0%
TCCATTAGGAAATGTTCCAG+TGG + chr1.3:60144506-60144525 MS.gene20986:CDS 40.0%
TGAGTTTAACACTGAAGGTG+GGG - chr1.3:60145241-60145260 None:intergenic 40.0%
TGTGGAAGGAACTGCTATAA+TGG - chr1.3:60144289-60144308 None:intergenic 40.0%
TGTTGAAGGCAAAAGAAGCA+TGG - chr1.3:60144722-60144741 None:intergenic 40.0%
TTAGCTCAGAAACTGCTGAA+GGG - chr1.3:60143588-60143607 None:intergenic 40.0%
TTTCATGCAGGTTCTTTGGT+GGG + chr1.3:60143382-60143401 MS.gene20986:intron 40.0%
TTTGTAGGGGAAATTGTGAC+AGG - chr1.3:60144315-60144334 None:intergenic 40.0%
! ATTGTTGGAATAGATGGTGG+AGG - chr1.3:60145034-60145053 None:intergenic 40.0%
! ATTGTTGGTGGAGCTATTGT+TGG - chr1.3:60145049-60145068 None:intergenic 40.0%
! CAGGTACAATGCCAGATTTT+GGG + chr1.3:60145274-60145293 MS.gene20986:CDS 40.0%
! CCAACATCTTCAGGAACTTT+TGG + chr1.3:60145103-60145122 MS.gene20986:CDS 40.0%
! GGTGTTGGAGATGATATCAT+TGG - chr1.3:60144962-60144981 None:intergenic 40.0%
! GGTTTTTCCTATCATGAGAG+AGG + chr1.3:60143403-60143422 MS.gene20986:CDS 40.0%
! GTATCTGAGGGTGTAATAAC+TGG - chr1.3:60144261-60144280 None:intergenic 40.0%
! GTTGAAGTTGATTGTGTAGG+TGG - chr1.3:60144983-60145002 None:intergenic 40.0%
! TCAGCATTGGATTATGCTTG+TGG + chr1.3:60144630-60144649 MS.gene20986:CDS 40.0%
! TCAGGTACAATGCCAGATTT+TGG + chr1.3:60145273-60145292 MS.gene20986:CDS 40.0%
! TTTAGCTCAGAAACTGCTGA+AGG - chr1.3:60143589-60143608 None:intergenic 40.0%
!! GAAGGAGCATTTGTGTTTGA+AGG - chr1.3:60144552-60144571 None:intergenic 40.0%
ACAAATGCTCCTTCTGCTCA+GGG + chr1.3:60144558-60144577 MS.gene20986:CDS 45.0%
ACAACTCCTACAACAGTTCC+AGG + chr1.3:60144435-60144454 MS.gene20986:CDS 45.0%
ACCACAGGAACATCTTTGCA+AGG - chr1.3:60143970-60143989 None:intergenic 45.0%
ACGAAGAAACTTTGCTCTGC+TGG - chr1.3:60143450-60143469 None:intergenic 45.0%
ACTGTTGTAGGAGTTGTAGC+TGG - chr1.3:60144432-60144451 None:intergenic 45.0%
AGCTTCCCTTCAATCAGCAT+TGG + chr1.3:60144617-60144636 MS.gene20986:CDS 45.0%
AGTGTCTCCTCTCTCATGAT+AGG - chr1.3:60143413-60143432 None:intergenic 45.0%
ATCATGAGAGAGGAGACACT+TGG + chr1.3:60143413-60143432 MS.gene20986:CDS 45.0%
CATTGCAGCTGGATTTGTAG+GGG - chr1.3:60144328-60144347 None:intergenic 45.0%
CTTTCATGCAGGTTCTTTGG+TGG + chr1.3:60143381-60143400 MS.gene20986:intron 45.0%
GAACTCAAAAGGGAAGAGGA+AGG + chr1.3:60144129-60144148 MS.gene20986:CDS 45.0%
GACGACGATGAAGATGATGA+CGG - chr1.3:60144923-60144942 None:intergenic 45.0%
GCATTGCAGCTGGATTTGTA+GGG - chr1.3:60144329-60144348 None:intergenic 45.0%
GCATTTGTGTTTGAAGGAGG+TGG - chr1.3:60144546-60144565 None:intergenic 45.0%
GGAACATTTCCTAATGGAGG+TGG - chr1.3:60144504-60144523 None:intergenic 45.0%
GGAGGTGGATAAGATGTTAC+TGG - chr1.3:60144468-60144487 None:intergenic 45.0%
GGATAAGATGTTACTGGAGG+TGG - chr1.3:60144483-60144502 None:intergenic 45.0%
GGTGGATAAGATGTTACTGG+AGG - chr1.3:60144465-60144484 None:intergenic 45.0%
GGTGGATAAGATGTTACTGG+TGG - chr1.3:60144465-60144484 None:intergenic 45.0%
GGTGTAATGGTTGGAGTTGT+TGG - chr1.3:60144393-60144412 None:intergenic 45.0%
GTAAAAGGTTTCAGAGCTCG+AGG - chr1.3:60145342-60145361 None:intergenic 45.0%
GTAATGGTTGGAGTTGTTGG+AGG - chr1.3:60144390-60144409 None:intergenic 45.0%
GTTGATTGTGTAGGTGGTGT+TGG - chr1.3:60144977-60144996 None:intergenic 45.0%
GTTGGAGTTGTTGGAGGTAA+AGG - chr1.3:60144384-60144403 None:intergenic 45.0%
GTTGTAGCTGGATTGAAGAC+TGG - chr1.3:60144420-60144439 None:intergenic 45.0%
TCAGATTCGAGCTTTCGTCA+CGG + chr1.3:60143471-60143490 MS.gene20986:CDS 45.0%
TCCTTGCAAAGATGTTCCTG+TGG + chr1.3:60143966-60143985 MS.gene20986:CDS 45.0%
! GAGGGTGTAATAACTGGTGT+TGG - chr1.3:60144255-60144274 None:intergenic 45.0%
! GCATGGTTCTGCAAAGTAAC+AGG - chr1.3:60144705-60144724 None:intergenic 45.0%
! GGAGCATTTGTGTTTGAAGG+AGG - chr1.3:60144549-60144568 None:intergenic 45.0%
!! AAAAGCATCGTTGCTAGCTG+TGG + chr1.3:60143646-60143665 MS.gene20986:CDS 45.0%
!! GGAACTTTTGGGTAAGTGAG+AGG + chr1.3:60145115-60145134 MS.gene20986:intron 45.0%
!! GGAGGTGTTGTTAGATTTGG+TGG - chr1.3:60145016-60145035 None:intergenic 45.0%
!! GGTGGAGGTGTTGTTAGATT+TGG - chr1.3:60145019-60145038 None:intergenic 45.0%
!! GGTGTTGTTAGATTTGGTGG+TGG - chr1.3:60145013-60145032 None:intergenic 45.0%
!!! CGAGCTCTGAAACCTTTTAC+TGG + chr1.3:60145342-60145361 MS.gene20986:CDS 45.0%
AACTCAAGCAACCCAGCTTC+AGG + chr1.3:60145255-60145274 MS.gene20986:CDS 50.0%
ACAGCTCCACCAACATCTTC+AGG + chr1.3:60145094-60145113 MS.gene20986:CDS 50.0%
ACTGTTACTGGCATTGCAGC+TGG - chr1.3:60144339-60144358 None:intergenic 50.0%
CACAAATGCTCCTTCTGCTC+AGG + chr1.3:60144557-60144576 MS.gene20986:CDS 50.0%
CAGAAGCAGCAAGATTAGCG+CGG - chr1.3:60143942-60143961 None:intergenic 50.0%
CCAACAAGTTGCGACTTTGG+AGG + chr1.3:60144765-60144784 MS.gene20986:CDS 50.0%
CCTCCAAAGTCGCAACTTGT+TGG - chr1.3:60144768-60144787 None:intergenic 50.0%
CTGAGGCTTGCAGTGACAAA+TGG - chr1.3:60145387-60145406 None:intergenic 50.0%
CTGGCATTGTACCTGAAGCT+GGG - chr1.3:60145269-60145288 None:intergenic 50.0%
GAGCTTTCGTCACGGATCAT+AGG + chr1.3:60143479-60143498 MS.gene20986:CDS 50.0%
GAGGCAGAAAGAAGCTTCCT+TGG - chr1.3:60143250-60143269 None:intergenic 50.0%
GCTCCAACAAGTTGCGACTT+TGG + chr1.3:60144762-60144781 MS.gene20986:CDS 50.0%
GCTGATTGAAGGGAAGCTTG+TGG - chr1.3:60144615-60144634 None:intergenic 50.0%
GGAACAGCAGGTGTAGTTGA+AGG - chr1.3:60144363-60144382 None:intergenic 50.0%
GGAGCTGTTGACATTGTTGG+TGG - chr1.3:60145061-60145080 None:intergenic 50.0%
GGAGCTGTTGGAATTGATGG+TGG - chr1.3:60145082-60145101 None:intergenic 50.0%
GGCATTGCAGCTGGATTTGT+AGG - chr1.3:60144330-60144349 None:intergenic 50.0%
GGGCTATCAGACCCAAAATC+TGG - chr1.3:60145288-60145307 None:intergenic 50.0%
GGTGGAGCTGTTGACATTGT+TGG - chr1.3:60145064-60145083 None:intergenic 50.0%
GGTGGAGCTGTTGGAATTGA+TGG - chr1.3:60145085-60145104 None:intergenic 50.0%
GTTGGAGGTAAAGGAACAGC+AGG - chr1.3:60144375-60144394 None:intergenic 50.0%
TCTGGCATTGTACCTGAAGC+TGG - chr1.3:60145270-60145289 None:intergenic 50.0%
TTGAAGGAGGTGGCTGTTGA+GGG - chr1.3:60144536-60144555 None:intergenic 50.0%
TTTGAAGGAGGTGGCTGTTG+AGG - chr1.3:60144537-60144556 None:intergenic 50.0%
! AGTTGGTGTGTGGCGAAAAC+AGG + chr1.3:60144585-60144604 MS.gene20986:CDS 50.0%
! GAAGATGTTGGTGGAGCTGT+TGG - chr1.3:60145094-60145113 None:intergenic 50.0%
!! AAGTCGCAACTTGTTGGAGC+TGG - chr1.3:60144762-60144781 None:intergenic 50.0%
CAACTCTGTCCCTGAGCAGA+AGG - chr1.3:60144570-60144589 None:intergenic 55.0%
CTTCTGCTCAGGGACAGAGT+TGG + chr1.3:60144568-60144587 MS.gene20986:CDS 55.0%
GCTGGATTGAAGACTGGTGC+CGG - chr1.3:60144414-60144433 None:intergenic 55.0%
GGCTGTTGAGGGTTTACCAC+TGG - chr1.3:60144525-60144544 None:intergenic 55.0%
GGTGCACCTGGAACTGTTGT+AGG - chr1.3:60144444-60144463 None:intergenic 55.0%
! GATGTTACTGGTGGTGCACC+TGG - chr1.3:60144456-60144475 None:intergenic 55.0%
!! ACTGGTGCCGGTGTAATGGT+TGG - chr1.3:60144402-60144421 None:intergenic 55.0%
!! GAAGACTGGTGCCGGTGTAA+TGG - chr1.3:60144406-60144425 None:intergenic 55.0%
!! GGAGGCGCTGCTATGTTAGT+TGG + chr1.3:60144783-60144802 MS.gene20986:CDS 55.0%
!! TCAGGGACAGAGTTGGTGTG+TGG + chr1.3:60144575-60144594 MS.gene20986:CDS 55.0%
! TTTCAGAGCTCGAGGGTGCG+AGG - chr1.3:60145334-60145353 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 60143243 60145419 60143243 ID=MS.gene20986
chr1.3 mRNA 60143243 60145419 60143243 ID=MS.gene20986.t1;Parent=MS.gene20986
chr1.3 exon 60143243 60143303 60143243 ID=MS.gene20986.t1.exon1;Parent=MS.gene20986.t1
chr1.3 CDS 60143243 60143303 60143243 ID=cds.MS.gene20986.t1;Parent=MS.gene20986.t1
chr1.3 exon 60143392 60144816 60143392 ID=MS.gene20986.t1.exon2;Parent=MS.gene20986.t1
chr1.3 CDS 60143392 60144816 60143392 ID=cds.MS.gene20986.t1;Parent=MS.gene20986.t1
chr1.3 exon 60144900 60145125 60144900 ID=MS.gene20986.t1.exon3;Parent=MS.gene20986.t1
chr1.3 CDS 60144900 60145125 60144900 ID=cds.MS.gene20986.t1;Parent=MS.gene20986.t1
chr1.3 exon 60145230 60145419 60145230 ID=MS.gene20986.t1.exon4;Parent=MS.gene20986.t1
chr1.3 CDS 60145230 60145419 60145230 ID=cds.MS.gene20986.t1;Parent=MS.gene20986.t1
Gene Sequence

>MS.gene20986

ATGGCCAAGGAAGCTTCTTTCTGCCTCTTGTTTCTCTCTTCTCTCTTAATTTCATGTTCTGGTAAGTACTTTACACTGATAAATGATAATAACACATAGTACTCATTTTTGTGCAGATTCTAATCATATATTATTTCACTTTCATGCAGGTTCTTTGGTGGGTTTTTCCTATCATGAGAGAGGAGACACTTGGACATCATTACTCCAGCAGAGCAAAGTTTCTTCGTCTCAGATTCGAGCTTTCGTCACGGATCATAGGATTTTGAGCACTCTTACAAACTCAAATGTGTTAGTTGATCTCTACTTAAACAAAAGCCAAGTTGAAAAATTCATTATTTCAAACCCTTCAGCAGTTTCTGAGCTAAAAGCTCAACTAGTGAACTTTCTTCCACATTTAAACATCAAAAGCATCGTTGCTAGCTGTGGAAGTGAGTGCTTAGTACAAAATGAGATACCTTTGATTATGCATGCTCTTAAATCAATTCATTCAATTCTCAGTGATCTTCACATAAGCAAGGAAGTAAAAATCTCGGTCGCATTTCCACTTCAAGTCTTGAGGAAGTTGAATGCATCACAAGAACACGAAATTTGTAGATTACTTTCATTTATAAAGGAAACGAAATCGTTTGTAATGATAGAAGATAGCATTGATGGAGAATTAAGTGTGAATGATCATTTTGTTCAAACAATTATCAACCGCGCTAATCTTGCTGCTTCTGTTCTTCCTTGCAAAGATGTTCCTGTGGTTCTTACAATCAAGAGTTCAGTTATACCAAGTTCAATAGAAGTAACTCGATTTAGTAAAAGAGTATCCAAATATTTAGAAGCAAAAAGAATAGCTGCATTATATGTAGAACTGCATACAACAGAAGATTCTTCAATGAAAGAACTCAAAAGGGAAGAGGAAGGTATGTTTCCTTTGTCTAGAAGAGAGATCTTAAGCAAATTCCACAGAAGAAATATTCTAGATAACACAAACAGTCCAACAAACACAGTTTACCCTACAAATCCAACACCAGTTATTACACCCTCAGATACTCCAACCATTATAGCAGTTCCTTCCACAAATCCTGTCACAATTTCCCCTACAAATCCAGCTGCAATGCCAGTAACAGTTCCTTCAACTACACCTGCTGTTCCTTTACCTCCAACAACTCCAACCATTACACCGGCACCAGTCTTCAATCCAGCTACAACTCCTACAACAGTTCCAGGTGCACCACCAGTAACATCTTATCCACCTCCAGTAACATCTTATCCACCTCCATTAGGAAATGTTCCAGTGGTAAACCCTCAACAGCCACCTCCTTCAAACACAAATGCTCCTTCTGCTCAGGGACAGAGTTGGTGTGTGGCGAAAACAGGTGCTCCACAAGCTTCCCTTCAATCAGCATTGGATTATGCTTGTGGAAATGGTGCTGATTGTTCTCAAATACAACAGGGTGCAAGTTGTTACAGTCCTGTTACTTTGCAGAACCATGCTTCTTTTGCCTTCAACAGTTACTATCAAAAGAAGCCAGCTCCAACAAGTTGCGACTTTGGAGGCGCTGCTATGTTAGTTGGCTCAAATCCAAGTAAGATTTTAACATTTAAATAACCCTTGTTTTTTCTTTCTTTCAATCTCATGCTTCAAAATATTGATGATCATTTTTAATAGGTTCTGGTTCTTGCATTTATCCGTCATCATCTTCATCGTCGTCGTCATCCTCAACTTCACCAATGATATCATCTCCAACACCACCTACACAATCAACTTCAACTTCAATACCACCACCAAATCTAACAACACCTCCACCATCTATTCCAACAATAGCTCCACCAACAATGTCAACAGCTCCACCATCAATTCCAACAGCTCCACCAACATCTTCAGGAACTTTTGGGTAAGTGAGAGGCAAGTTTTTCAGTGACTTTAATAATTATATTCTGTGATACCAATAAGCATGTACTAATCATGGTTCTGTATAAACAATGCTGTCTTTTGTAGCTATGGTACCCCACCTTCAGTGTTAAACTCAAGCAACCCAGCTTCAGGTACAATGCCAGATTTTGGGTCTGATAGCCCTCCTATTGTAAACACAACATCGGCCTCGCACCCTCGAGCTCTGAAACCTTTTACTGGCTGTATAGTCTTGATGATTCCATTTGTCACTGCAAGCCTCAGCATGCACCTATAG
Protein sequence

>MS.gene20986.t1

MAKEASFCLLFLSSLLISCSGSLVGFSYHERGDTWTSLLQQSKVSSSQIRAFVTDHRILSTLTNSNVLVDLYLNKSQVEKFIISNPSAVSELKAQLVNFLPHLNIKSIVASCGSECLVQNEIPLIMHALKSIHSILSDLHISKEVKISVAFPLQVLRKLNASQEHEICRLLSFIKETKSFVMIEDSIDGELSVNDHFVQTIINRANLAASVLPCKDVPVVLTIKSSVIPSSIEVTRFSKRVSKYLEAKRIAALYVELHTTEDSSMKELKREEEGMFPLSRREILSKFHRRNILDNTNSPTNTVYPTNPTPVITPSDTPTIIAVPSTNPVTISPTNPAAMPVTVPSTTPAVPLPPTTPTITPAPVFNPATTPTTVPGAPPVTSYPPPVTSYPPPLGNVPVVNPQQPPPSNTNAPSAQGQSWCVAKTGAPQASLQSALDYACGNGADCSQIQQGASCYSPVTLQNHASFAFNSYYQKKPAPTSCDFGGAAMLVGSNPSSGSCIYPSSSSSSSSSSTSPMISSPTPPTQSTSTSIPPPNLTTPPPSIPTIAPPTMSTAPPSIPTAPPTSSGTFGYGTPPSVLNSSNPASGTMPDFGSDSPPIVNTTSASHPRALKPFTGCIVLMIPFVTASLSMHL