Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050707.t1 | KEH24650.1 | 95.1 | 246 | 9 | 2 | 3 | 245 | 69 | 314 | 6.00E-136 | 493.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050707.t1 | O74507 | 23.4 | 252 | 159 | 9 | 4 | 227 | 80 | 325 | 2.9e-06 | 53.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050707.t1 | A0A072UFT9 | 95.1 | 246 | 9 | 2 | 3 | 245 | 69 | 314 | 4.3e-136 | 493.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050338 | MS.gene050707 | 0.81683 | 4.52E-52 | -1.69E-46 |
| MS.gene050707 | MS.gene050710 | 0.801886 | 7.29E-49 | -1.69E-46 |
| MS.gene050707 | MS.gene052308 | 0.815495 | 8.98E-52 | -1.69E-46 |
| MS.gene050707 | MS.gene054850 | 0.881041 | 3.34E-70 | -1.69E-46 |
| MS.gene050707 | MS.gene054941 | 0.837197 | 5.99E-57 | -1.69E-46 |
| MS.gene050707 | MS.gene05652 | 0.833581 | 4.91E-56 | -1.69E-46 |
| MS.gene050707 | MS.gene057669 | 0.817014 | 4.11E-52 | -1.69E-46 |
| MS.gene050707 | MS.gene058128 | 0.831606 | 1.52E-55 | -1.69E-46 |
| MS.gene050707 | MS.gene058853 | 0.846785 | 1.75E-59 | -1.69E-46 |
| MS.gene050707 | MS.gene05926 | 0.824863 | 6.41E-54 | -1.69E-46 |
| MS.gene050707 | MS.gene059616 | 0.826064 | 3.33E-54 | -1.69E-46 |
| MS.gene050707 | MS.gene059905 | 0.845034 | 5.22E-59 | -1.69E-46 |
| MS.gene050707 | MS.gene061357 | 0.807697 | 4.45E-50 | -1.69E-46 |
| MS.gene050707 | MS.gene061366 | 0.85007 | 2.15E-60 | -1.69E-46 |
| MS.gene050707 | MS.gene063035 | 0.810272 | 1.25E-50 | -1.69E-46 |
| MS.gene050707 | MS.gene063037 | 0.801305 | 9.59E-49 | -1.69E-46 |
| MS.gene050707 | MS.gene063279 | 0.830519 | 2.81E-55 | -1.69E-46 |
| MS.gene050707 | MS.gene063280 | 0.821594 | 3.72E-53 | -1.69E-46 |
| MS.gene050707 | MS.gene063734 | 0.825722 | 4.01E-54 | -1.69E-46 |
| MS.gene050707 | MS.gene06421 | 0.838731 | 2.42E-57 | -1.69E-46 |
| MS.gene050707 | MS.gene064328 | 0.813448 | 2.55E-51 | -1.69E-46 |
| MS.gene050707 | MS.gene064509 | 0.83394 | 3.99E-56 | -1.69E-46 |
| MS.gene050707 | MS.gene065292 | 0.827305 | 1.68E-54 | -1.69E-46 |
| MS.gene050707 | MS.gene070324 | 0.813715 | 2.22E-51 | -1.69E-46 |
| MS.gene050707 | MS.gene070726 | 0.810834 | 9.46E-51 | -1.69E-46 |
| MS.gene050707 | MS.gene072366 | 0.839338 | 1.68E-57 | -1.69E-46 |
| MS.gene050707 | MS.gene074201 | 0.824109 | 9.64E-54 | -1.69E-46 |
| MS.gene050707 | MS.gene074515 | 0.841316 | 5.12E-58 | -1.69E-46 |
| MS.gene050707 | MS.gene08362 | 0.840782 | 7.07E-58 | -1.69E-46 |
| MS.gene050707 | MS.gene20121 | -0.813241 | 2.83E-51 | -1.69E-46 |
| MS.gene050707 | MS.gene20986 | 0.822086 | 2.86E-53 | -1.69E-46 |
| MS.gene050707 | MS.gene21055 | 0.820903 | 5.36E-53 | -1.69E-46 |
| MS.gene050707 | MS.gene21734 | 0.813 | 3.19E-51 | -1.69E-46 |
| MS.gene050707 | MS.gene21888 | 0.81774 | 2.82E-52 | -1.69E-46 |
| MS.gene050707 | MS.gene22383 | 0.800134 | 1.66E-48 | -1.69E-46 |
| MS.gene050707 | MS.gene22759 | 0.998453 | 1.59E-265 | -1.69E-46 |
| MS.gene050707 | MS.gene24720 | 0.820147 | 8.00E-53 | -1.69E-46 |
| MS.gene050707 | MS.gene25729 | 0.824387 | 8.30E-54 | -1.69E-46 |
| MS.gene050707 | MS.gene25797 | 0.840892 | 6.61E-58 | -1.69E-46 |
| MS.gene050707 | MS.gene26122 | 0.816736 | 4.74E-52 | -1.69E-46 |
| MS.gene050707 | MS.gene26901 | 0.837733 | 4.37E-57 | -1.69E-46 |
| MS.gene050707 | MS.gene27560 | 0.833414 | 5.41E-56 | -1.69E-46 |
| MS.gene050707 | MS.gene27811 | 0.809669 | 1.69E-50 | -1.69E-46 |
| MS.gene050707 | MS.gene27812 | 0.811228 | 7.78E-51 | -1.69E-46 |
| MS.gene050707 | MS.gene28923 | 0.838134 | 3.44E-57 | -1.69E-46 |
| MS.gene050707 | MS.gene29215 | 0.83575 | 1.40E-56 | -1.69E-46 |
| MS.gene050707 | MS.gene29939 | 0.814172 | 1.76E-51 | -1.69E-46 |
| MS.gene050707 | MS.gene29940 | 0.840866 | 6.72E-58 | -1.69E-46 |
| MS.gene050707 | MS.gene29942 | 0.84593 | 2.99E-59 | -1.69E-46 |
| MS.gene050707 | MS.gene31804 | 0.816375 | 5.71E-52 | -1.69E-46 |
| MS.gene050707 | MS.gene31917 | 0.876427 | 1.41E-68 | -1.69E-46 |
| MS.gene050707 | MS.gene31973 | 0.880031 | 7.67E-70 | -1.69E-46 |
| MS.gene050707 | MS.gene32680 | 0.800895 | 1.16E-48 | -1.69E-46 |
| MS.gene050707 | MS.gene33809 | 0.878132 | 3.60E-69 | -1.69E-46 |
| MS.gene050707 | MS.gene34976 | 0.825428 | 4.71E-54 | -1.69E-46 |
| MS.gene050707 | MS.gene35794 | 0.806983 | 6.31E-50 | -1.69E-46 |
| MS.gene050707 | MS.gene35795 | 0.821958 | 3.06E-53 | -1.69E-46 |
| MS.gene050707 | MS.gene35853 | 0.807381 | 5.20E-50 | -1.69E-46 |
| MS.gene050707 | MS.gene35931 | 0.82651 | 2.61E-54 | -1.69E-46 |
| MS.gene050707 | MS.gene38358 | 0.806742 | 7.10E-50 | -1.69E-46 |
| MS.gene050707 | MS.gene39469 | 0.86293 | 3.58E-64 | -1.69E-46 |
| MS.gene050707 | MS.gene40278 | 0.838209 | 3.30E-57 | -1.69E-46 |
| MS.gene050707 | MS.gene40724 | 0.8135 | 2.48E-51 | -1.69E-46 |
| MS.gene050707 | MS.gene40749 | 0.827969 | 1.17E-54 | -1.69E-46 |
| MS.gene050707 | MS.gene41190 | 0.845681 | 3.49E-59 | -1.69E-46 |
| MS.gene050707 | MS.gene42726 | 0.80686 | 6.70E-50 | -1.69E-46 |
| MS.gene050707 | MS.gene48363 | 0.836004 | 1.21E-56 | -1.69E-46 |
| MS.gene050707 | MS.gene49425 | 0.803692 | 3.09E-49 | -1.69E-46 |
| MS.gene050707 | MS.gene49744 | 0.810429 | 1.16E-50 | -1.69E-46 |
| MS.gene050707 | MS.gene51554 | 0.801093 | 1.06E-48 | -1.69E-46 |
| MS.gene050707 | MS.gene51960 | 0.80351 | 3.37E-49 | -1.69E-46 |
| MS.gene050707 | MS.gene52072 | 0.841217 | 5.43E-58 | -1.69E-46 |
| MS.gene050707 | MS.gene52113 | 0.819062 | 1.41E-52 | -1.69E-46 |
| MS.gene050707 | MS.gene52363 | 0.804303 | 2.30E-49 | -1.69E-46 |
| MS.gene050707 | MS.gene52403 | 0.816719 | 4.78E-52 | -1.69E-46 |
| MS.gene050707 | MS.gene53408 | 0.817903 | 2.59E-52 | -1.69E-46 |
| MS.gene050707 | MS.gene54335 | 0.875707 | 2.49E-68 | -1.69E-46 |
| MS.gene050707 | MS.gene56303 | 0.810736 | 9.94E-51 | -1.69E-46 |
| MS.gene050707 | MS.gene58849 | 0.857638 | 1.42E-62 | -1.69E-46 |
| MS.gene050707 | MS.gene61094 | 0.822789 | 1.96E-53 | -1.69E-46 |
| MS.gene050707 | MS.gene61684 | 0.807012 | 6.22E-50 | -1.69E-46 |
| MS.gene050707 | MS.gene63375 | 0.829873 | 4.03E-55 | -1.69E-46 |
| MS.gene050707 | MS.gene64210 | 0.802788 | 4.75E-49 | -1.69E-46 |
| MS.gene050707 | MS.gene69722 | 0.824708 | 6.97E-54 | -1.69E-46 |
| MS.gene050707 | MS.gene70403 | 0.828623 | 8.11E-55 | -1.69E-46 |
| MS.gene050707 | MS.gene70493 | 0.83405 | 3.75E-56 | -1.69E-46 |
| MS.gene050707 | MS.gene71022 | 0.804926 | 1.71E-49 | -1.69E-46 |
| MS.gene050707 | MS.gene73896 | 0.819007 | 1.46E-52 | -1.69E-46 |
| MS.gene050707 | MS.gene74253 | 0.802865 | 4.58E-49 | -1.69E-46 |
| MS.gene050707 | MS.gene74548 | 0.856625 | 2.84E-62 | -1.69E-46 |
| MS.gene050707 | MS.gene77771 | 0.827098 | 1.89E-54 | -1.69E-46 |
| MS.gene050707 | MS.gene79461 | 0.815788 | 7.73E-52 | -1.69E-46 |
| MS.gene050707 | MS.gene80899 | 0.844286 | 8.30E-59 | -1.69E-46 |
| MS.gene050707 | MS.gene82050 | -0.811685 | 6.19E-51 | -1.69E-46 |
| MS.gene050707 | MS.gene87345 | 0.814056 | 1.87E-51 | -1.69E-46 |
| MS.gene050707 | MS.gene90842 | 0.807367 | 5.23E-50 | -1.69E-46 |
| MS.gene050707 | MS.gene90984 | 0.800923 | 1.15E-48 | -1.69E-46 |
| MS.gene050707 | MS.gene92313 | 0.801902 | 7.23E-49 | -1.69E-46 |
| MS.gene050707 | MS.gene94553 | 0.83556 | 1.56E-56 | -1.69E-46 |
| MS.gene050707 | MS.gene94954 | 0.84547 | 3.98E-59 | -1.69E-46 |
| MS.gene050707 | MS.gene97443 | 0.873305 | 1.63E-67 | -1.69E-46 |
| MS.gene050707 | MS.gene99885 | 0.823366 | 1.44E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050707.t1 | MTR_7g117475 | 95.122 | 246 | 9 | 2 | 3 | 245 | 69 | 314 | 3.26e-171 | 474 |
| MS.gene050707.t1 | MTR_8g023760 | 70.518 | 251 | 62 | 3 | 3 | 245 | 83 | 329 | 1.78e-127 | 364 |
| MS.gene050707.t1 | MTR_8g107030 | 34.444 | 180 | 100 | 7 | 57 | 227 | 87 | 257 | 1.20e-14 | 72.4 |
| MS.gene050707.t1 | MTR_8g107030 | 33.702 | 181 | 102 | 7 | 57 | 228 | 118 | 289 | 2.07e-14 | 72.0 |
| MS.gene050707.t1 | MTR_8g107030 | 33.143 | 175 | 99 | 7 | 57 | 222 | 87 | 252 | 4.45e-13 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050707.t1 | AT2G46890 | 64.940 | 251 | 76 | 3 | 3 | 245 | 76 | 322 | 7.92e-119 | 342 |
| MS.gene050707.t1 | AT1G18180 | 33.171 | 205 | 114 | 8 | 30 | 223 | 61 | 253 | 1.79e-15 | 74.3 |
| MS.gene050707.t1 | AT1G18180 | 32.683 | 205 | 115 | 8 | 30 | 223 | 61 | 253 | 2.05e-15 | 74.3 |
| MS.gene050707.t1 | AT1G73650 | 32.258 | 217 | 129 | 9 | 30 | 238 | 61 | 267 | 9.36e-13 | 66.6 |
| MS.gene050707.t1 | AT1G73650 | 32.258 | 217 | 129 | 9 | 30 | 238 | 61 | 267 | 9.95e-13 | 67.0 |
| MS.gene050707.t1 | AT1G73650 | 32.258 | 217 | 129 | 9 | 30 | 238 | 61 | 267 | 1.08e-12 | 66.6 |
| MS.gene050707.t1 | AT1G73650 | 35.099 | 151 | 89 | 5 | 96 | 238 | 36 | 185 | 1.73e-12 | 65.1 |
| MS.gene050707.t1 | AT1G73650 | 35.099 | 151 | 89 | 5 | 96 | 238 | 36 | 185 | 2.11e-12 | 64.7 |
Find 75 sgRNAs with CRISPR-Local
Find 77 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTGGCTCACTATGATTTC+TGG | 0.120398 | 7.4:+273993 | MS.gene050707:CDS |
| TGCAGATGATTGACCCTTAT+TGG | 0.153787 | 7.4:+273927 | MS.gene050707:CDS |
| GGGCATGGGTGGACCTTTAT+AGG | 0.223333 | 7.4:+274460 | MS.gene050707:CDS |
| TACTGAGTGGATAACATAAA+AGG | 0.250782 | 7.4:-274221 | None:intergenic |
| TGGTGGGGACTTGTAGTATT+TGG | 0.269007 | 7.4:+274427 | MS.gene050707:CDS |
| CATTCTTTGTTCCACAAGTT+TGG | 0.288006 | 7.4:-274518 | None:intergenic |
| GCTGTTGTTGTGTGTGTATC+TGG | 0.295142 | 7.4:+274274 | MS.gene050707:CDS |
| ACTGATATGATTCAACAATA+TGG | 0.297749 | 7.4:+274124 | MS.gene050707:CDS |
| ACAGGTATCACTGTCCAATA+AGG | 0.308159 | 7.4:-273941 | None:intergenic |
| ATTGCTCAACACAATGTGTT+TGG | 0.338794 | 7.4:+274486 | MS.gene050707:CDS |
| TTGGTTGGAACTTGGGGCAT+GGG | 0.350925 | 7.4:+274446 | MS.gene050707:CDS |
| TAAATCAGCCTTTGAACATC+TGG | 0.357916 | 7.4:+274242 | MS.gene050707:CDS |
| AGCTGCTCACCAAAGTAATT+AGG | 0.361585 | 7.4:-274400 | None:intergenic |
| CCTCACCAGTTATTGCTCAT+TGG | 0.368116 | 7.4:+274187 | MS.gene050707:CDS |
| AAGGGTGGGCTGCATAATAA+TGG | 0.377206 | 7.4:-273973 | None:intergenic |
| TGTAGTATTTGGTTGGAACT+TGG | 0.385747 | 7.4:+274438 | MS.gene050707:CDS |
| TTATTATGCAGCCCACCCTT+TGG | 0.386930 | 7.4:+273976 | MS.gene050707:CDS |
| TTGCTCAACACAATGTGTTT+GGG | 0.407007 | 7.4:+274487 | MS.gene050707:CDS |
| TTTGGTTGGAACTTGGGGCA+TGG | 0.421477 | 7.4:+274445 | MS.gene050707:CDS |
| GTAGTATTTGGTTGGAACTT+GGG | 0.421705 | 7.4:+274439 | MS.gene050707:CDS |
| ACAAAAGAGTAGAAGCATAT+AGG | 0.425663 | 7.4:+274551 | MS.gene050707:CDS |
| TACTCCACACCCAAGTCAAC+AGG | 0.431420 | 7.4:-274033 | None:intergenic |
| GCCTTTGAACATCTGGGACT+TGG | 0.435472 | 7.4:+274249 | MS.gene050707:CDS |
| AATGGGGTGCAAGGGAAGAT+TGG | 0.438963 | 7.4:+274095 | MS.gene050707:CDS |
| TTTGTGAATAACAATGGTAA+TGG | 0.445517 | 7.4:+274337 | MS.gene050707:CDS |
| ACAATCCAATGAGCAATAAC+TGG | 0.447606 | 7.4:-274192 | None:intergenic |
| TAGTATTTGGTTGGAACTTG+GGG | 0.450578 | 7.4:+274440 | MS.gene050707:CDS |
| GTTGGAACTTGGGGCATGGG+TGG | 0.451857 | 7.4:+274449 | MS.gene050707:CDS |
| AGGTAGGTAGATGGAGAAGA+AGG | 0.452132 | 7.4:-274167 | None:intergenic |
| GTTGAGCAATGCTCCTATAA+AGG | 0.453733 | 7.4:-274473 | None:intergenic |
| ATTACTTTGGTGAGCAGCTA+TGG | 0.459668 | 7.4:+274404 | MS.gene050707:CDS |
| GGGGACTTGTAGTATTTGGT+TGG | 0.460375 | 7.4:+274431 | MS.gene050707:CDS |
| ACTCGGCATCCTAATTACTT+TGG | 0.476296 | 7.4:+274391 | MS.gene050707:CDS |
| CAGACGAGAGAAGTGGCAAT+GGG | 0.479915 | 7.4:+274078 | MS.gene050707:CDS |
| ACTATGATTTCTGGAGATCA+AGG | 0.489125 | 7.4:+274002 | MS.gene050707:CDS |
| TGGTGAGCAGCTATGGTGGT+GGG | 0.489748 | 7.4:+274411 | MS.gene050707:CDS |
| TAACTGGTGAGGTAGGTAGA+TGG | 0.495738 | 7.4:-274176 | None:intergenic |
| ATCAGAACACAACTTCTGTA+TGG | 0.498222 | 7.4:+274578 | MS.gene050707:CDS |
| ACTGAGTGGATAACATAAAA+GGG | 0.503941 | 7.4:-274220 | None:intergenic |
| TTGGTGAGCAGCTATGGTGG+TGG | 0.505228 | 7.4:+274410 | MS.gene050707:CDS |
| CAGGTATCACTGTCCAATAA+GGG | 0.506881 | 7.4:-273940 | None:intergenic |
| AGATGAGTTGAACTTGAAGC+AGG | 0.514693 | 7.4:-274605 | None:intergenic |
| AGAAGTGGCAATGGGGTGCA+AGG | 0.516645 | 7.4:+274086 | MS.gene050707:CDS |
| AACAATATGGAAAACATTGG+TGG | 0.523523 | 7.4:+274137 | MS.gene050707:CDS |
| TGATCCTTGAGAGTGGTTTG+TGG | 0.530278 | 7.4:+274362 | MS.gene050707:CDS |
| AATATGGAAAACATTGGTGG+TGG | 0.539715 | 7.4:+274140 | MS.gene050707:CDS |
| TAATAATGGACAAGCATCAC+AGG | 0.547440 | 7.4:-273959 | None:intergenic |
| GGTGAGCAGCTATGGTGGTG+GGG | 0.554190 | 7.4:+274412 | MS.gene050707:CDS |
| TGATCCTGTTGACTTGGGTG+TGG | 0.558189 | 7.4:+274029 | MS.gene050707:CDS |
| GGGTTATGTGACCAAACTTG+TGG | 0.558732 | 7.4:+274507 | MS.gene050707:CDS |
| GGATAGTGATCCTGTTGACT+TGG | 0.567382 | 7.4:+274023 | MS.gene050707:CDS |
| CAGAAATCATAGTGAGCCAA+AGG | 0.576012 | 7.4:-273992 | None:intergenic |
| TCTCTCGTCTGAAGTAGTTG+TGG | 0.584105 | 7.4:-274066 | None:intergenic |
| AAATCAGCCTTTGAACATCT+GGG | 0.586715 | 7.4:+274243 | MS.gene050707:CDS |
| ACCAAGTCCCAGATGTTCAA+AGG | 0.590896 | 7.4:-274250 | None:intergenic |
| GATGAGTTGAACTTGAAGCA+GGG | 0.598199 | 7.4:-274604 | None:intergenic |
| TGAGCAATAACTGGTGAGGT+AGG | 0.598678 | 7.4:-274183 | None:intergenic |
| AGAAATCATAGTGAGCCAAA+GGG | 0.603318 | 7.4:-273991 | None:intergenic |
| GAAGTGGCAATGGGGTGCAA+GGG | 0.603591 | 7.4:+274087 | MS.gene050707:CDS |
| ATCATAGTGAGCCAAAGGGT+GGG | 0.605285 | 7.4:-273987 | None:intergenic |
| TTCAACAATATGGAAAACAT+TGG | 0.608096 | 7.4:+274134 | MS.gene050707:CDS |
| TCAGACGAGAGAAGTGGCAA+TGG | 0.611058 | 7.4:+274077 | MS.gene050707:CDS |
| ATGAGTTGAACTTGAAGCAG+GGG | 0.615221 | 7.4:-274603 | None:intergenic |
| AATCATAGTGAGCCAAAGGG+TGG | 0.615234 | 7.4:-273988 | None:intergenic |
| AGTACCACAAACCACTCTCA+AGG | 0.625195 | 7.4:-274366 | None:intergenic |
| ATATGGAAAACATTGGTGGT+GGG | 0.626188 | 7.4:+274141 | MS.gene050707:CDS |
| TCAAAGGCTGATTTACTGAG+TGG | 0.626720 | 7.4:-274234 | None:intergenic |
| GTGGTTTGTGGTACTATACT+CGG | 0.626740 | 7.4:+274374 | MS.gene050707:CDS |
| ACTACTTCAGACGAGAGAAG+TGG | 0.633464 | 7.4:+274071 | MS.gene050707:CDS |
| GATAGTGATCCTGTTGACTT+GGG | 0.636502 | 7.4:+274024 | MS.gene050707:CDS |
| CCAATGAGCAATAACTGGTG+AGG | 0.640552 | 7.4:-274187 | None:intergenic |
| ACTTTGGTGAGCAGCTATGG+TGG | 0.658770 | 7.4:+274407 | MS.gene050707:CDS |
| TATCTGCAAAGTATGCTATG+AGG | 0.672642 | 7.4:-274300 | None:intergenic |
| AATGGTATGATCCTTGAGAG+TGG | 0.682971 | 7.4:+274355 | MS.gene050707:CDS |
| AGACGAGAGAAGTGGCAATG+GGG | 0.720125 | 7.4:+274079 | MS.gene050707:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TACAATTTTGTGAATAACAA+TGG | + | chr7.4:274331-274350 | MS.gene050707:CDS | 20.0% |
| ! | ACTGATATGATTCAACAATA+TGG | + | chr7.4:274124-274143 | MS.gene050707:CDS | 25.0% |
| ! | TTCAACAATATGGAAAACAT+TGG | + | chr7.4:274134-274153 | MS.gene050707:CDS | 25.0% |
| ! | TTTGTGAATAACAATGGTAA+TGG | + | chr7.4:274337-274356 | MS.gene050707:CDS | 25.0% |
| AACAATATGGAAAACATTGG+TGG | + | chr7.4:274137-274156 | MS.gene050707:CDS | 30.0% | |
| ACTGAGTGGATAACATAAAA+GGG | - | chr7.4:274223-274242 | None:intergenic | 30.0% | |
| TACTGAGTGGATAACATAAA+AGG | - | chr7.4:274224-274243 | None:intergenic | 30.0% | |
| ! | ACAAAAGAGTAGAAGCATAT+AGG | + | chr7.4:274551-274570 | MS.gene050707:CDS | 30.0% |
| !!! | GCATTCTTAGTTTTTTTGAG+TGG | - | chr7.4:274640-274659 | None:intergenic | 30.0% |
| AAATCAGCCTTTGAACATCT+GGG | + | chr7.4:274243-274262 | MS.gene050707:CDS | 35.0% | |
| AATATGGAAAACATTGGTGG+TGG | + | chr7.4:274140-274159 | MS.gene050707:CDS | 35.0% | |
| ACAATCCAATGAGCAATAAC+TGG | - | chr7.4:274195-274214 | None:intergenic | 35.0% | |
| AGAAATCATAGTGAGCCAAA+GGG | - | chr7.4:273994-274013 | None:intergenic | 35.0% | |
| ATCAGAACACAACTTCTGTA+TGG | + | chr7.4:274578-274597 | MS.gene050707:CDS | 35.0% | |
| ATTGCTCAACACAATGTGTT+TGG | + | chr7.4:274486-274505 | MS.gene050707:CDS | 35.0% | |
| CATTCTTTGTTCCACAAGTT+TGG | - | chr7.4:274521-274540 | None:intergenic | 35.0% | |
| GTAGTATTTGGTTGGAACTT+GGG | + | chr7.4:274439-274458 | MS.gene050707:CDS | 35.0% | |
| TAAATCAGCCTTTGAACATC+TGG | + | chr7.4:274242-274261 | MS.gene050707:CDS | 35.0% | |
| TAGTATTTGGTTGGAACTTG+GGG | + | chr7.4:274440-274459 | MS.gene050707:CDS | 35.0% | |
| TATCTGCAAAGTATGCTATG+AGG | - | chr7.4:274303-274322 | None:intergenic | 35.0% | |
| TGTAGTATTTGGTTGGAACT+TGG | + | chr7.4:274438-274457 | MS.gene050707:CDS | 35.0% | |
| TTGCTCAACACAATGTGTTT+GGG | + | chr7.4:274487-274506 | MS.gene050707:CDS | 35.0% | |
| ! | ACTATGATTTCTGGAGATCA+AGG | + | chr7.4:274002-274021 | MS.gene050707:CDS | 35.0% |
| ! | ATATGGAAAACATTGGTGGT+GGG | + | chr7.4:274141-274160 | MS.gene050707:CDS | 35.0% |
| !! | TAATAATGGACAAGCATCAC+AGG | - | chr7.4:273962-273981 | None:intergenic | 35.0% |
| AATGGTATGATCCTTGAGAG+TGG | + | chr7.4:274355-274374 | MS.gene050707:CDS | 40.0% | |
| ACAGGTATCACTGTCCAATA+AGG | - | chr7.4:273944-273963 | None:intergenic | 40.0% | |
| ACTCGGCATCCTAATTACTT+TGG | + | chr7.4:274391-274410 | MS.gene050707:CDS | 40.0% | |
| AGCTGCTCACCAAAGTAATT+AGG | - | chr7.4:274403-274422 | None:intergenic | 40.0% | |
| ATTACTTTGGTGAGCAGCTA+TGG | + | chr7.4:274404-274423 | MS.gene050707:CDS | 40.0% | |
| CAGAAATCATAGTGAGCCAA+AGG | - | chr7.4:273995-274014 | None:intergenic | 40.0% | |
| CAGGTATCACTGTCCAATAA+GGG | - | chr7.4:273943-273962 | None:intergenic | 40.0% | |
| GTTGAGCAATGCTCCTATAA+AGG | - | chr7.4:274476-274495 | None:intergenic | 40.0% | |
| TGCAGATGATTGACCCTTAT+TGG | + | chr7.4:273927-273946 | MS.gene050707:CDS | 40.0% | |
| ! | AGATGAGTTGAACTTGAAGC+AGG | - | chr7.4:274608-274627 | None:intergenic | 40.0% |
| ! | ATGAGTTGAACTTGAAGCAG+GGG | - | chr7.4:274606-274625 | None:intergenic | 40.0% |
| ! | CTTTGGCTCACTATGATTTC+TGG | + | chr7.4:273993-274012 | MS.gene050707:CDS | 40.0% |
| ! | GATGAGTTGAACTTGAAGCA+GGG | - | chr7.4:274607-274626 | None:intergenic | 40.0% |
| ! | GTGGTTTGTGGTACTATACT+CGG | + | chr7.4:274374-274393 | MS.gene050707:CDS | 40.0% |
| ! | TCAAAGGCTGATTTACTGAG+TGG | - | chr7.4:274237-274256 | None:intergenic | 40.0% |
| !! | GATAGTGATCCTGTTGACTT+GGG | + | chr7.4:274024-274043 | MS.gene050707:CDS | 40.0% |
| AATCATAGTGAGCCAAAGGG+TGG | - | chr7.4:273991-274010 | None:intergenic | 45.0% | |
| ACCAAGTCCCAGATGTTCAA+AGG | - | chr7.4:274253-274272 | None:intergenic | 45.0% | |
| ACTACTTCAGACGAGAGAAG+TGG | + | chr7.4:274071-274090 | MS.gene050707:CDS | 45.0% | |
| AGGTAGGTAGATGGAGAAGA+AGG | - | chr7.4:274170-274189 | None:intergenic | 45.0% | |
| AGTACCACAAACCACTCTCA+AGG | - | chr7.4:274369-274388 | None:intergenic | 45.0% | |
| ATCATAGTGAGCCAAAGGGT+GGG | - | chr7.4:273990-274009 | None:intergenic | 45.0% | |
| CCAATGAGCAATAACTGGTG+AGG | - | chr7.4:274190-274209 | None:intergenic | 45.0% | |
| CCTCACCAGTTATTGCTCAT+TGG | + | chr7.4:274187-274206 | MS.gene050707:CDS | 45.0% | |
| GCTGTTGTTGTGTGTGTATC+TGG | + | chr7.4:274274-274293 | MS.gene050707:CDS | 45.0% | |
| GGGGACTTGTAGTATTTGGT+TGG | + | chr7.4:274431-274450 | MS.gene050707:CDS | 45.0% | |
| GGGTTATGTGACCAAACTTG+TGG | + | chr7.4:274507-274526 | MS.gene050707:CDS | 45.0% | |
| TAACTGGTGAGGTAGGTAGA+TGG | - | chr7.4:274179-274198 | None:intergenic | 45.0% | |
| TGAGCAATAACTGGTGAGGT+AGG | - | chr7.4:274186-274205 | None:intergenic | 45.0% | |
| TGATCCTTGAGAGTGGTTTG+TGG | + | chr7.4:274362-274381 | MS.gene050707:CDS | 45.0% | |
| TGGTGGGGACTTGTAGTATT+TGG | + | chr7.4:274427-274446 | MS.gene050707:CDS | 45.0% | |
| TTATTATGCAGCCCACCCTT+TGG | + | chr7.4:273976-273995 | MS.gene050707:CDS | 45.0% | |
| ! | GGATAGTGATCCTGTTGACT+TGG | + | chr7.4:274023-274042 | MS.gene050707:CDS | 45.0% |
| ! | TCTCTCGTCTGAAGTAGTTG+TGG | - | chr7.4:274069-274088 | None:intergenic | 45.0% |
| !! | AAGGGTGGGCTGCATAATAA+TGG | - | chr7.4:273976-273995 | None:intergenic | 45.0% |
| AATGGGGTGCAAGGGAAGAT+TGG | + | chr7.4:274095-274114 | MS.gene050707:CDS | 50.0% | |
| ACTTTGGTGAGCAGCTATGG+TGG | + | chr7.4:274407-274426 | MS.gene050707:CDS | 50.0% | |
| AGACGAGAGAAGTGGCAATG+GGG | + | chr7.4:274079-274098 | MS.gene050707:CDS | 50.0% | |
| CAGACGAGAGAAGTGGCAAT+GGG | + | chr7.4:274078-274097 | MS.gene050707:CDS | 50.0% | |
| TACTCCACACCCAAGTCAAC+AGG | - | chr7.4:274036-274055 | None:intergenic | 50.0% | |
| TCAGACGAGAGAAGTGGCAA+TGG | + | chr7.4:274077-274096 | MS.gene050707:CDS | 50.0% | |
| TTGGTTGGAACTTGGGGCAT+GGG | + | chr7.4:274446-274465 | MS.gene050707:CDS | 50.0% | |
| TTTGGTTGGAACTTGGGGCA+TGG | + | chr7.4:274445-274464 | MS.gene050707:CDS | 50.0% | |
| ! | GCCTTTGAACATCTGGGACT+TGG | + | chr7.4:274249-274268 | MS.gene050707:CDS | 50.0% |
| !! | TGATCCTGTTGACTTGGGTG+TGG | + | chr7.4:274029-274048 | MS.gene050707:CDS | 50.0% |
| AGAAGTGGCAATGGGGTGCA+AGG | + | chr7.4:274086-274105 | MS.gene050707:CDS | 55.0% | |
| GAAGTGGCAATGGGGTGCAA+GGG | + | chr7.4:274087-274106 | MS.gene050707:CDS | 55.0% | |
| GGGCATGGGTGGACCTTTAT+AGG | + | chr7.4:274460-274479 | MS.gene050707:CDS | 55.0% | |
| TGGTGAGCAGCTATGGTGGT+GGG | + | chr7.4:274411-274430 | MS.gene050707:CDS | 55.0% | |
| TTGGTGAGCAGCTATGGTGG+TGG | + | chr7.4:274410-274429 | MS.gene050707:CDS | 55.0% | |
| GTTGGAACTTGGGGCATGGG+TGG | + | chr7.4:274449-274468 | MS.gene050707:CDS | 60.0% | |
| ! | GGTGAGCAGCTATGGTGGTG+GGG | + | chr7.4:274412-274431 | MS.gene050707:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 273926 | 274663 | 273926 | ID=MS.gene050707 |
| chr7.4 | mRNA | 273926 | 274663 | 273926 | ID=MS.gene050707.t1;Parent=MS.gene050707 |
| chr7.4 | exon | 273926 | 274663 | 273926 | ID=MS.gene050707.t1.exon1;Parent=MS.gene050707.t1 |
| chr7.4 | CDS | 273926 | 274663 | 273926 | ID=cds.MS.gene050707.t1;Parent=MS.gene050707.t1 |
| Gene Sequence |
| Protein sequence |
