AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21055

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21055.t1 MTR_1g084820 94.945 633 32 0 1 633 1 633 0.0 1019
MS.gene21055.t1 MTR_1g084820 94.571 571 31 0 1 571 1 571 0.0 900
MS.gene21055.t1 MTR_7g111600 63.402 194 50 8 318 503 64 244 3.84e-56 193
MS.gene21055.t1 MTR_7g111600 63.402 194 50 8 318 503 64 244 6.11e-56 190
MS.gene21055.t1 MTR_7g111600 63.402 194 50 8 318 503 64 244 1.76e-55 191
MS.gene21055.t1 MTR_3g048280 47.468 158 60 4 346 502 60 195 7.36e-36 134
MS.gene21055.t1 MTR_5g091650 53.636 110 50 1 402 510 90 199 4.16e-31 122
MS.gene21055.t1 MTR_5g091650 56.383 94 40 1 413 505 101 194 1.11e-29 116
MS.gene21055.t1 MTR_2g049670 41.121 214 83 6 331 511 46 249 1.85e-29 119
MS.gene21055.t1 MTR_2g049670 48.031 127 49 2 420 546 158 267 3.13e-25 107
MS.gene21055.t1 MTR_8g089400 53.636 110 47 3 405 512 24 131 1.39e-28 112
MS.gene21055.t1 MTR_8g089400 53.636 110 47 3 405 512 44 151 1.95e-28 112
MS.gene21055.t1 MTR_1g056640 57.609 92 37 2 416 505 402 493 1.64e-27 117
MS.gene21055.t1 MTR_5g015720 25.617 527 254 18 1 502 1 414 3.00e-27 115
MS.gene21055.t1 MTR_8g085390 45.038 131 60 2 419 548 29 148 6.60e-27 108
MS.gene21055.t1 MTR_4g132280 50.485 103 49 2 412 512 374 476 4.34e-26 112
MS.gene21055.t1 MTR_8g085440 51.685 89 42 1 416 503 28 116 2.43e-25 101
MS.gene21055.t1 MTR_8g088120 62.821 78 28 1 429 505 57 134 2.62e-25 105
MS.gene21055.t1 MTR_8g085400 59.494 79 30 1 418 496 31 107 3.30e-24 98.2
MS.gene21055.t1 MTR_5g085580 52.128 94 43 2 414 505 376 469 3.09e-22 100
MS.gene21055.t1 MTR_8g085420 48.810 84 42 1 416 498 28 111 9.22e-22 92.0
MS.gene21055.t1 MTR_3g083580 46.000 100 52 2 418 515 360 459 9.90e-22 99.4
MS.gene21055.t1 MTR_5g097420 51.613 93 43 2 412 502 22 114 1.25e-21 90.9
MS.gene21055.t1 MTR_8g085720 47.368 95 48 2 420 512 364 458 1.10e-20 96.3
MS.gene21055.t1 MTR_5g078200 47.917 96 48 2 412 505 319 414 1.49e-20 96.3
MS.gene21055.t1 MTR_5g078200 47.917 96 48 2 412 505 352 447 1.52e-20 96.3
MS.gene21055.t1 MTR_6g088885 38.739 111 65 2 408 515 357 467 1.86e-20 95.5
MS.gene21055.t1 MTR_3g082080 53.846 78 35 1 420 496 157 234 2.23e-20 91.3
MS.gene21055.t1 MTR_4g083500 41.000 100 58 1 418 516 400 499 2.56e-20 95.1
MS.gene21055.t1 MTR_1g022160 47.778 90 46 1 419 507 390 479 3.25e-20 94.7
MS.gene21055.t1 MTR_4g079530 44.554 101 54 2 410 508 27 127 6.44e-20 86.3
MS.gene21055.t1 MTR_3g065460 40.984 122 70 2 397 516 338 459 1.15e-19 92.8
MS.gene21055.t1 MTR_4g109490 52.941 85 38 2 419 502 23 106 1.24e-19 88.6
MS.gene21055.t1 MTR_4g133735 48.352 91 45 2 414 502 22 112 1.34e-19 85.1
MS.gene21055.t1 MTR_1g116870 45.455 88 47 1 416 502 272 359 1.40e-19 91.3
MS.gene21055.t1 MTR_4g079530 49.474 95 46 2 420 512 37 131 1.65e-19 87.0
MS.gene21055.t1 MTR_5g044530 50.000 92 43 2 417 505 19 110 1.67e-19 89.0
MS.gene21055.t1 MTR_4g083070 50.000 88 42 2 420 505 21 108 5.72e-19 85.9
MS.gene21055.t1 MTR_1g022160 48.235 85 43 1 419 502 390 474 1.49e-18 89.4
MS.gene21055.t1 MTR_8g102340 38.182 110 66 2 419 526 333 442 1.62e-18 89.4
MS.gene21055.t1 MTR_4g133730 46.154 91 47 2 414 502 22 112 1.76e-18 82.0
MS.gene21055.t1 MTR_4g069940 45.133 113 53 4 403 507 353 464 1.77e-18 89.0
MS.gene21055.t1 MTR_2g072500 47.674 86 43 2 418 502 6 90 1.95e-18 80.9
MS.gene21055.t1 MTR_1g031900 40.517 116 67 2 418 531 359 474 4.11e-18 88.2
MS.gene21055.t1 MTR_1g031900 48.276 87 43 2 418 502 359 445 1.02e-17 87.0
MS.gene21055.t1 MTR_1g022160 47.500 80 41 1 419 497 390 469 3.42e-17 85.1
MS.gene21055.t1 MTR_8g012400 39.216 102 55 3 405 504 364 460 2.28e-16 82.4
MS.gene21055.t1 MTR_8g012400 39.216 102 55 3 405 504 284 380 2.29e-16 82.0
MS.gene21055.t1 MTR_7g053500 42.478 113 60 5 395 504 273 383 4.05e-16 81.3
MS.gene21055.t1 MTR_3g116510 42.202 109 58 3 403 507 218 325 6.72e-16 79.7
MS.gene21055.t1 MTR_7g053500 42.478 113 60 5 395 504 350 460 6.92e-16 80.9
MS.gene21055.t1 MTR_3g116510 42.202 109 58 3 403 507 352 459 8.79e-16 80.9
MS.gene21055.t1 MTR_7g026340 38.235 102 62 1 400 500 345 446 5.67e-14 75.1
MS.gene21055.t1 MTR_4g088155 39.623 106 57 4 402 504 358 459 1.64e-13 73.6
MS.gene21055.t1 MTR_3g031770 31.333 150 87 5 400 535 342 489 1.46e-12 70.9
MS.gene21055.t1 MTR_1g053320 35.849 106 58 5 418 518 372 472 7.79e-12 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21055.t1 AT1G09460 69.048 126 36 2 386 509 101 225 9.31e-44 160
MS.gene21055.t1 AT2G30933 59.574 94 37 1 418 510 80 173 4.47e-33 127
MS.gene21055.t1 AT1G29380 64.444 90 31 1 420 508 148 237 1.60e-31 124
MS.gene21055.t1 AT1G29380 65.476 84 28 1 420 502 148 231 3.04e-30 121
MS.gene21055.t1 AT2G30933 43.846 130 41 2 368 496 61 159 8.72e-29 112
MS.gene21055.t1 AT2G30933 43.846 130 41 2 368 496 61 159 8.72e-29 112
MS.gene21055.t1 AT1G29380 66.667 78 25 1 420 496 148 225 2.08e-28 114
MS.gene21055.t1 AT1G29380 66.667 78 25 1 420 496 148 225 2.08e-28 114
MS.gene21055.t1 AT2G05790 53.448 116 49 3 395 505 356 471 2.96e-28 119
MS.gene21055.t1 AT1G11820 51.515 99 46 2 418 514 379 477 1.58e-25 111
MS.gene21055.t1 AT4G13600 44.094 127 66 3 420 542 61 186 2.13e-25 105
MS.gene21055.t1 AT4G05430 44.444 117 63 2 400 514 3 119 2.65e-25 102
MS.gene21055.t1 AT4G05430 44.167 120 65 2 397 514 25 144 7.05e-25 102
MS.gene21055.t1 AT5G55180 52.632 95 43 2 413 505 365 459 1.31e-24 107
MS.gene21055.t1 AT4G26830 35.751 193 110 6 322 505 267 454 1.06e-23 105
MS.gene21055.t1 AT3G55430 46.739 92 48 1 412 502 358 449 1.27e-23 104
MS.gene21055.t1 AT4G26830 35.751 193 110 6 322 505 271 458 1.55e-23 104
MS.gene21055.t1 AT5G55180 55.814 86 36 2 413 496 365 450 2.60e-22 100
MS.gene21055.t1 AT5G35740 52.747 91 40 3 415 502 25 115 2.88e-22 92.8
MS.gene21055.t1 AT2G01630 39.535 129 74 3 418 544 358 484 1.57e-21 99.0
MS.gene21055.t1 AT4G34480 47.674 86 44 1 419 503 252 337 1.57e-21 97.8
MS.gene21055.t1 AT1G18650 47.778 90 46 1 419 507 20 109 2.04e-21 92.8
MS.gene21055.t1 AT4G34480 47.674 86 44 1 419 503 363 448 2.56e-21 98.2
MS.gene21055.t1 AT3G58100 47.872 94 47 2 420 511 41 134 5.71e-21 91.3
MS.gene21055.t1 AT1G11820 54.321 81 35 2 418 496 379 459 6.86e-21 96.7
MS.gene21055.t1 AT3G58100 46.939 98 50 2 416 511 113 210 9.00e-21 92.8
MS.gene21055.t1 AT1G18650 47.191 89 46 1 419 506 20 108 1.35e-20 88.6
MS.gene21055.t1 AT4G05430 46.078 102 53 2 397 496 25 126 1.51e-20 88.2
MS.gene21055.t1 AT1G79480 50.000 84 41 1 420 502 310 393 3.66e-20 93.6
MS.gene21055.t1 AT1G79480 50.000 84 41 1 420 502 311 394 3.66e-20 93.6
MS.gene21055.t1 AT1G66250 39.474 114 66 3 418 529 366 478 5.58e-20 94.0
MS.gene21055.t1 AT2G16230 39.806 103 61 1 419 520 365 467 1.14e-19 93.2
MS.gene21055.t1 AT5G61130 47.674 86 44 1 419 503 20 105 1.34e-19 87.8
MS.gene21055.t1 AT1G69295 56.338 71 29 2 419 488 20 89 2.43e-19 87.8
MS.gene21055.t1 AT1G69295 56.338 71 29 2 419 488 16 85 4.23e-19 87.0
MS.gene21055.t1 AT5G67460 48.235 85 42 2 420 502 293 377 4.46e-19 90.1
MS.gene21055.t1 AT5G56590 40.777 103 59 2 420 520 369 471 9.86e-19 90.1
MS.gene21055.t1 AT1G26450 38.312 154 67 6 421 550 22 171 1.06e-18 85.1
MS.gene21055.t1 AT5G63225 46.512 86 45 1 418 502 23 108 1.43e-18 82.0
MS.gene21055.t1 AT1G66870 42.529 87 48 2 418 502 51 137 2.52e-18 82.4
MS.gene21055.t1 AT1G66870 42.529 87 48 2 418 502 24 110 2.80e-18 81.3
MS.gene21055.t1 AT5G67460 50.633 79 37 2 420 496 293 371 2.88e-18 87.8
MS.gene21055.t1 AT4G29360 45.977 87 45 2 418 502 399 485 3.00e-18 89.0
MS.gene21055.t1 AT4G29360 45.977 87 45 2 418 502 389 475 3.09e-18 89.0
MS.gene21055.t1 AT4G09462 41.758 91 52 1 415 504 24 114 4.44e-18 80.9
MS.gene21055.t1 AT5G24318 36.697 109 62 2 398 505 217 319 5.18e-18 85.9
MS.gene21055.t1 AT5G24318 36.697 109 62 2 398 505 356 458 5.83e-18 87.4
MS.gene21055.t1 AT4G09465 40.659 91 53 1 415 504 26 116 8.56e-18 80.1
MS.gene21055.t1 AT5G08000 44.262 122 66 2 417 536 37 158 9.33e-18 82.8
MS.gene21055.t1 AT4G09467 40.659 91 53 1 415 504 26 116 1.05e-17 79.7
MS.gene21055.t1 AT4G09464 40.659 91 53 1 415 504 26 116 1.05e-17 79.7
MS.gene21055.t1 AT3G13560 43.434 99 54 2 420 516 362 460 1.14e-17 87.0
MS.gene21055.t1 AT3G13560 43.434 99 54 2 420 516 362 460 1.14e-17 87.0
MS.gene21055.t1 AT3G13560 43.434 99 54 2 420 516 362 460 1.14e-17 87.0
MS.gene21055.t1 AT3G13560 43.434 99 54 2 420 516 362 460 1.14e-17 87.0
MS.gene21055.t1 AT3G13560 43.434 99 54 2 420 516 362 460 1.14e-17 87.0
MS.gene21055.t1 AT4G09466 40.659 91 53 1 415 504 26 116 2.22e-17 79.0
MS.gene21055.t1 AT2G42930 44.872 78 43 0 420 497 40 117 2.93e-17 79.0
MS.gene21055.t1 AT4G09090 43.023 86 48 1 420 504 31 116 3.21e-17 78.6
MS.gene21055.t1 AT2G01630 48.148 81 40 2 418 496 358 438 3.54e-17 85.1
MS.gene21055.t1 AT1G66852 39.535 86 51 1 418 502 24 109 5.48e-17 77.4
MS.gene21055.t1 AT3G23770 44.118 102 53 4 405 504 374 473 9.54e-17 84.0
MS.gene21055.t1 AT5G08000 47.674 86 44 1 419 503 20 105 9.65e-17 79.3
MS.gene21055.t1 AT1G78520 40.000 90 52 2 414 502 26 114 1.67e-16 76.3
MS.gene21055.t1 AT2G04910 43.182 88 48 2 415 500 10 97 1.87e-16 75.9
MS.gene21055.t1 AT5G63240 36.275 102 61 2 407 504 27 128 7.00e-16 75.1
MS.gene21055.t1 AT5G24318 37.000 100 56 2 398 496 356 449 1.32e-15 80.1
MS.gene21055.t1 AT5G63230 37.363 91 56 1 413 502 19 109 3.97e-15 72.4
MS.gene21055.t1 AT1G66855 37.931 87 52 2 418 502 24 110 6.15e-15 71.6
MS.gene21055.t1 AT5G63250 38.776 98 55 3 412 504 33 130 8.61e-15 72.0
MS.gene21055.t1 AT4G16165 37.931 87 52 2 418 502 24 110 2.05e-14 70.1
MS.gene21055.t1 AT2G43670 38.298 94 55 3 410 502 26 117 4.17e-14 69.7
MS.gene21055.t1 AT5G53600 39.535 86 50 2 418 502 27 111 5.32e-14 68.9
MS.gene21055.t1 AT2G04910 43.678 87 46 3 412 496 8 93 6.91e-14 69.3
MS.gene21055.t1 AT2G39640 34.091 88 57 1 419 505 459 546 8.49e-14 74.7
MS.gene21055.t1 AT2G39640 35.417 96 55 2 406 500 351 440 3.25e-12 69.7
MS.gene21055.t1 AT2G43660 41.667 96 52 4 409 502 26 119 1.34e-13 68.2
MS.gene21055.t1 AT2G43660 41.667 96 52 4 409 502 25 118 1.62e-13 68.2
MS.gene21055.t1 AT3G07320 39.216 102 58 3 405 504 358 457 3.74e-13 72.4
MS.gene21055.t1 AT2G29735 39.785 93 53 2 413 502 24 116 6.46e-13 66.2
MS.gene21055.t1 AT4G14080 37.255 102 62 2 405 504 374 475 7.22e-13 71.6
MS.gene21055.t1 AT1G13830 39.130 92 47 3 417 502 32 120 8.62e-13 68.6
MS.gene21055.t1 AT1G13830 39.326 89 45 3 420 502 21 106 1.28e-12 67.4
MS.gene21055.t1 AT2G03505 36.667 90 54 2 419 505 20 109 1.50e-12 65.1
MS.gene21055.t1 AT2G03505 36.667 90 54 2 419 505 20 109 1.77e-12 66.2
MS.gene21055.t1 AT2G03505 36.667 90 54 2 419 505 20 109 1.77e-12 66.2
MS.gene21055.t1 AT2G03505 36.667 90 54 2 419 505 20 109 1.77e-12 66.2
MS.gene21055.t1 AT5G53610 41.463 82 46 2 420 500 28 108 2.29e-12 64.3
MS.gene21055.t1 AT5G64790 38.144 97 56 4 418 512 367 461 5.64e-12 68.9

Find 141 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGGTACAATGCCAGATTT+TGG 0.194586 1.4:-68575792 MS.gene21055:CDS
CCAACATCTTCAGGCACTTT+TGG 0.216484 1.4:-68575962 MS.gene21055:CDS
TCACTTTCATGCAGGTTCTT+TGG 0.248475 1.4:-68577687 MS.gene21055:intron
TCTCTCTTAATTTCATGTTC+TGG 0.269637 1.4:-68577783 MS.gene21055:intron
TAGATTACTTTCATTTATAA+AGG 0.277901 1.4:-68577231 MS.gene21055:CDS
GGTGGAGCTGTTGGAATTGA+TGG 0.308099 1.4:+68575983 None:intergenic
AAGACTATACAGCCAGTAAA+AGG 0.355621 1.4:+68575711 None:intergenic
GGAGGCGCTGCTATGTTAGT+TGG 0.360601 1.4:-68576282 MS.gene21055:CDS
ATGAAGAAACTTTGCTCTGC+TGG 0.376113 1.4:+68577618 None:intergenic
GGAGGTGGATAAGATGTTAC+TGG 0.377508 1.4:+68576579 None:intergenic
ATGATAGAAGATAGCATTGA+TGG 0.377602 1.4:-68577191 MS.gene21055:CDS
CCTCCAAAGTCGCAACTTGT+TGG 0.380624 1.4:+68576300 None:intergenic
GGTGTAATGGTTGGAGTTGT+TGG 0.382964 1.4:+68576675 None:intergenic
GGTGGAGGTGTTGTTAGATT+TGG 0.383669 1.4:+68576049 None:intergenic
GGCATTGCAGCTGGATTTGT+AGG 0.384777 1.4:+68576738 None:intergenic
TGTGGAAGGAACTGCTATAA+TGG 0.388341 1.4:+68576779 None:intergenic
AACTCAAGCAACCCAGCTTC+AGG 0.391337 1.4:-68575810 MS.gene21055:CDS
GCTCCAACAAGTTGCGACTT+TGG 0.392668 1.4:-68576303 MS.gene21055:CDS
ATAACTGGTGTTGGATTTGT+AGG 0.394170 1.4:+68576822 None:intergenic
GCATGGTTCTGCAAAGTAAC+AGG 0.394899 1.4:+68576363 None:intergenic
GAGCTTTCGTCACAGATCAT+AGG 0.396108 1.4:-68577586 MS.gene21055:CDS
GGAGGTGTTGTTAGATTTGG+TGG 0.400113 1.4:+68576052 None:intergenic
CGAGTTACTTCTATTGAACT+TGG 0.401043 1.4:+68577050 None:intergenic
TTGGATTATGCTTGTGGAAA+TGG 0.401736 1.4:-68576429 MS.gene21055:CDS
CTTCAATGAAAGAACTCAAA+AGG 0.413209 1.4:-68576947 MS.gene21055:CDS
GTAGTTGAAGGAACTGTTAC+TGG 0.413784 1.4:+68576717 None:intergenic
GAAGGAGCATTTGTGTTTGA+AGG 0.413852 1.4:+68576516 None:intergenic
GTTGGAGTTGTTGGAGGTAA+AGG 0.413921 1.4:+68576684 None:intergenic
TTGAAGGAGGTGGCTGTTGA+GGG 0.417203 1.4:+68576532 None:intergenic
CACAAATGCTCCTTCTGCTC+AGG 0.420750 1.4:-68576508 MS.gene21055:CDS
ACAGCTCCACCAACATCTTC+AGG 0.424830 1.4:-68575971 MS.gene21055:CDS
ACCACTGGAACATTTCCTAA+TGG 0.424925 1.4:+68576558 None:intergenic
GGATAAGATGTTACTGGAGG+TGG 0.426667 1.4:+68576585 None:intergenic
GCCGATGTTGTGTTTACAAT+AGG 0.430103 1.4:+68575756 None:intergenic
ACTGTTACTGGCATTGCAGC+TGG 0.430772 1.4:+68576729 None:intergenic
TGATTGTTCTCAAATACAAC+AGG 0.433295 1.4:-68576403 MS.gene21055:CDS
ACAACTCCTACAACAGTTCC+AGG 0.436358 1.4:-68576630 MS.gene21055:CDS
ACATCTTATCCACCTCCATT+AGG 0.436623 1.4:-68576573 MS.gene21055:CDS
CTTTCATGCAGGTTCTTTGG+TGG 0.439102 1.4:-68577684 MS.gene21055:intron
GGAAATTGTGACAGGATTTG+TGG 0.439347 1.4:+68576761 None:intergenic
GGAGCTATTGTTGGAATAGA+TGG 0.441593 1.4:+68576028 None:intergenic
GGGCTATCAGACCCAAAATC+TGG 0.444861 1.4:+68575780 None:intergenic
TTTCATGCAGGTTCTTTGGT+GGG 0.448012 1.4:-68577683 MS.gene21055:intron
TTTGAAGGAGGTGGCTGTTG+AGG 0.448619 1.4:+68576531 None:intergenic
GGTGGAGCTGTTGACATTGT+TGG 0.450114 1.4:+68576004 None:intergenic
AGCTTCCCTTCAATCAGCAT+TGG 0.454325 1.4:-68576448 MS.gene21055:CDS
AACTTCCTCAAGACTTGAAG+TGG 0.458266 1.4:+68577281 None:intergenic
CTGAGGCTTGCAGTGACAAA+TGG 0.467298 1.4:+68575681 None:intergenic
ATTGTTGGTGGAGCTATTGT+TGG 0.472956 1.4:+68576019 None:intergenic
AGTTGGTGTGTGGCGAAAAC+AGG 0.475765 1.4:-68576480 MS.gene21055:CDS
AAGTCGCAACTTGTTGGAGC+TGG 0.478203 1.4:+68576306 None:intergenic
GAGGCAGAAAGAAGCTTCCT+TGG 0.478234 1.4:+68577818 None:intergenic
TCTGGCATTGTACCTGAAGC+TGG 0.478959 1.4:+68575798 None:intergenic
GGTGCACCTGGAACTGTTGT+AGG 0.485248 1.4:+68576624 None:intergenic
GTAGGGTAAACTGTGTTTGT+TGG 0.486254 1.4:+68576840 None:intergenic
GGAGGTGGATAAGATGTTAC+TGG 0.487201 1.4:+68576600 None:intergenic
GGCTGTTGAGGGTTTACCAC+TGG 0.491135 1.4:+68576543 None:intergenic
CATAATCCAATGCTGATTGA+AGG 0.493090 1.4:+68576442 None:intergenic
GTTGATTGTGTAGGTGGTGT+TGG 0.494210 1.4:+68576091 None:intergenic
TTTGTAGGGGAAATTGTGAC+AGG 0.494725 1.4:+68576753 None:intergenic
TATCTAGAATATTTCTTCTG+TGG 0.496929 1.4:+68576874 None:intergenic
AGTGTCTCCTCTCTCATGAT+AGG 0.500543 1.4:+68577655 None:intergenic
GTATCTGAGGGTGTAATAAC+TGG 0.502389 1.4:+68576807 None:intergenic
GCATTGCAGCTGGATTTGTA+GGG 0.503176 1.4:+68576739 None:intergenic
GATGTTACTGGTGGTGCACC+TGG 0.511282 1.4:+68576612 None:intergenic
TTCAATGAAAGAACTCAAAA+GGG 0.511868 1.4:-68576946 MS.gene21055:CDS
CTTGAGTTTAACACTGAAGG+TGG 0.514620 1.4:+68575825 None:intergenic
TCAGCATTGGATTATGCTTG+TGG 0.514748 1.4:-68576435 MS.gene21055:CDS
GCATTTGTGTTTGAAGGAGG+TGG 0.517825 1.4:+68576522 None:intergenic
CTCTTGATTGTAAGAACCAC+AGG 0.520516 1.4:+68577083 None:intergenic
CATTTCCACTTCAAGTCTTG+AGG 0.520651 1.4:-68577286 MS.gene21055:CDS
GGAGCATTTGTGTTTGAAGG+AGG 0.521515 1.4:+68576519 None:intergenic
GAAGACTGGTGCCGGTGTAA+TGG 0.528319 1.4:+68576662 None:intergenic
GATGTTGTGTTTACAATAGG+AGG 0.530245 1.4:+68575759 None:intergenic
CAACTCTGTCCCTGAGCAGA+AGG 0.531643 1.4:+68576498 None:intergenic
TCCTTGCAAAGATGTTCCTG+TGG 0.532621 1.4:-68577099 MS.gene21055:CDS
CCAACAAGTTGCGACTTTGG+AGG 0.533263 1.4:-68576300 MS.gene21055:CDS
GAAGATGTTGGTGGAGCTGT+TGG 0.534788 1.4:+68575974 None:intergenic
GGAACAGCAGGTGTAGTTGA+AGG 0.536488 1.4:+68576705 None:intergenic
GAAAGAACTCAAAAGGGAAG+AGG 0.546037 1.4:-68576940 MS.gene21055:CDS
GGAACATTTCCTAATGGAGG+TGG 0.552963 1.4:+68576564 None:intergenic
GTTGGAGGTAAAGGAACAGC+AGG 0.553488 1.4:+68576693 None:intergenic
TCTTACAAACTCAAATGTGT+TGG 0.554054 1.4:-68577552 MS.gene21055:CDS
GGAGCTGTTGACATTGTTGG+TGG 0.559801 1.4:+68576007 None:intergenic
GAGGGTGTAATAACTGGTGT+TGG 0.563477 1.4:+68576813 None:intergenic
ATAATCCAATGCTGATTGAA+GGG 0.564193 1.4:+68576443 None:intergenic
TTTAACTCGGAAACTGCTGA+AGG 0.564291 1.4:+68577479 None:intergenic
ACTGTTGTAGGAGTTGTAGC+TGG 0.566759 1.4:+68576636 None:intergenic
GGTGTTGTTAGATTTGGTGG+TGG 0.572595 1.4:+68576055 None:intergenic
TTGAGTTTAACACTGAAGGT+GGG 0.573230 1.4:+68575826 None:intergenic
TCTCTACTTAAACAAAAGCC+AGG 0.575198 1.4:-68577525 MS.gene21055:CDS
TAACTGGTGTTGGATTTGTA+GGG 0.580532 1.4:+68576823 None:intergenic
CTTCTGCTCAGGGACAGAGT+TGG 0.581714 1.4:-68576497 MS.gene21055:CDS
TTGCTTGAGTTTAACACTGA+AGG 0.581994 1.4:+68575822 None:intergenic
TTAAGAGCATGCATAATCAA+AGG 0.588835 1.4:+68577368 None:intergenic
TTTCAGAGCTCGAGGGTGCG+AGG 0.590599 1.4:+68575734 None:intergenic
TATAATGGTTGGAGTATCTG+AGG 0.591925 1.4:+68576794 None:intergenic
GAAGGAACTGCTATAATGGT+TGG 0.591976 1.4:+68576783 None:intergenic
AAGATCTCTCTTCTAGACAA+AGG 0.594620 1.4:+68576906 None:intergenic
GGTGTTGGAGATGATATCAT+TGG 0.598845 1.4:+68576106 None:intergenic
ATACTCTCTGATAATGGCCA+AGG 0.605015 1.4:-68577835 None:intergenic
GTAATGGTTGGAGTTGTTGG+AGG 0.606112 1.4:+68576678 None:intergenic
ATCATGAGAGAGGAGACACT+TGG 0.607322 1.4:-68577652 MS.gene21055:CDS
GTAAAAGGTTTCAGAGCTCG+AGG 0.607544 1.4:+68575726 None:intergenic
TGAGTTTAACACTGAAGGTG+GGG 0.608299 1.4:+68575827 None:intergenic
GAAGTTGAAGTTGATTGTGT+AGG 0.609053 1.4:+68576082 None:intergenic
TGATATCATTGGTGAAGTTG+AGG 0.609455 1.4:+68576117 None:intergenic
GTTGAAGTTGATTGTGTAGG+TGG 0.611559 1.4:+68576085 None:intergenic
ATGTTGTGTTTACAATAGGA+GGG 0.618672 1.4:+68575760 None:intergenic
GTTGTAGCTGGATTGAAGAC+TGG 0.620005 1.4:+68576648 None:intergenic
ACAAATGCTCCTTCTGCTCA+GGG 0.620260 1.4:-68576507 MS.gene21055:CDS
ATTGTGACAGGATTTGTGGA+AGG 0.620979 1.4:+68576765 None:intergenic
TTAACTCGGAAACTGCTGAA+GGG 0.622484 1.4:+68577480 None:intergenic
AGAGAGAAGAGAGAAACAAG+AGG 0.623621 1.4:+68577799 None:intergenic
GCTGGATTGAAGACTGGTGC+CGG 0.624496 1.4:+68576654 None:intergenic
AACAACTCCAACCATTACAC+CGG 0.624559 1.4:-68576673 MS.gene21055:CDS
GGAGCTGTTGGAATTGATGG+TGG 0.624833 1.4:+68575986 None:intergenic
ACTGGTGCCGGTGTAATGGT+TGG 0.625957 1.4:+68576666 None:intergenic
CTGGCATTGTACCTGAAGCT+GGG 0.626526 1.4:+68575799 None:intergenic
CAGTGATCTTCACATAAGCA+AGG 0.629071 1.4:-68577327 MS.gene21055:CDS
ACCACAGGAACATCTTTGCA+AGG 0.631919 1.4:+68577098 None:intergenic
AACTCTATAGGTGCATGCTG+AGG 0.632489 1.4:+68575664 None:intergenic
CATTGCAGCTGGATTTGTAG+GGG 0.635434 1.4:+68576740 None:intergenic
GAACTCAAAAGGGAAGAGGA+AGG 0.639773 1.4:-68576936 MS.gene21055:CDS
CCAAAAGTGCCTGAAGATGT+TGG 0.642640 1.4:+68575962 None:intergenic
TGTTGAAGGCAAAAGAAGCA+TGG 0.645483 1.4:+68576346 None:intergenic
GCTATTGTTGGAATAGATGG+TGG 0.647261 1.4:+68576031 None:intergenic
TCCATTAGGAAATGTTCCAG+TGG 0.658091 1.4:-68576559 MS.gene21055:CDS
ACTGGAACATTTCCTAATGG+AGG 0.658231 1.4:+68576561 None:intergenic
GCTGATTGAAGGGAAGCTTG+TGG 0.661755 1.4:+68576453 None:intergenic
TAAAAGGTTTCAGAGCTCGA+GGG 0.668012 1.4:+68575727 None:intergenic
ATTGTTGGAATAGATGGTGG+AGG 0.673696 1.4:+68576034 None:intergenic
TCCTATTGTAAACACAACAT+CGG 0.678848 1.4:-68575757 MS.gene21055:CDS
CAGAAGCAGCAAGATTAGCG+CGG 0.682382 1.4:+68577126 None:intergenic
AAAGTGCCTGAAGATGTTGG+TGG 0.686382 1.4:+68575965 None:intergenic
ATAATGGTTGGAGTATCTGA+GGG 0.698374 1.4:+68576795 None:intergenic
TCAGGGACAGAGTTGGTGTG+TGG 0.715688 1.4:-68576490 MS.gene21055:CDS
GGTGGATAAGATGTTACTGG+AGG 0.717889 1.4:+68576582 None:intergenic
GATTGTTCTCAAATACAACA+GGG 0.719905 1.4:-68576402 MS.gene21055:CDS
GACGACGATGAAGATGATGA+CGG 0.723295 1.4:+68576145 None:intergenic
GGTGGATAAGATGTTACTGG+TGG 0.740803 1.4:+68576603 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGATTACTTTCATTTATAA+AGG - chr1.4:68576259-68576278 MS.gene21055:intron 15.0%
!! TTAAATGTTAAAATCTTACT+TGG + chr1.4:68577241-68577260 None:intergenic 15.0%
!!! TATTGATGATCATTTTTAAT+AGG - chr1.4:68577303-68577322 MS.gene21055:CDS 15.0%
!! ATTGAAAGAAAGAAAAAACA+AGG + chr1.4:68577269-68577288 None:intergenic 20.0%
!! TTGAAAGAAAGAAAAAACAA+GGG + chr1.4:68577268-68577287 None:intergenic 20.0%
!!! ATGTTTTTGATGTTTAAATG+TGG + chr1.4:68576059-68576078 None:intergenic 20.0%
!!! TCTTTTTGCTTCTAAATATT+TGG + chr1.4:68576483-68576502 None:intergenic 20.0%
! ATATTATTTCACTTTCATGC+AGG - chr1.4:68575795-68575814 MS.gene21055:CDS 25.0%
! TTCAATGAAAGAACTCAAAA+GGG - chr1.4:68576544-68576563 MS.gene21055:CDS 25.0%
!! TATCTAGAATATTTCTTCTG+TGG + chr1.4:68576619-68576638 None:intergenic 25.0%
!!! AAGTAATGAATTTTTCAACC+TGG + chr1.4:68575986-68576005 None:intergenic 25.0%
!!! TGATCATTTTTAATAGGTTC+TGG - chr1.4:68577309-68577328 MS.gene21055:CDS 25.0%
AAGCAAGGAAGTAAAAATCT+CGG - chr1.4:68576178-68576197 MS.gene21055:intron 30.0%
ATGTTGTGTTTACAATAGGA+GGG + chr1.4:68577733-68577752 None:intergenic 30.0%
CAATAAGCATGTACTAATCA+TGG - chr1.4:68577603-68577622 MS.gene21055:CDS 30.0%
CATGATTAGTACATGCTTAT+TGG + chr1.4:68577605-68577624 None:intergenic 30.0%
CTTCAATGAAAGAACTCAAA+AGG - chr1.4:68576543-68576562 MS.gene21055:CDS 30.0%
GATTGTTCTCAAATACAACA+GGG - chr1.4:68577088-68577107 MS.gene21055:CDS 30.0%
TCCTATTGTAAACACAACAT+CGG - chr1.4:68577733-68577752 MS.gene21055:intron 30.0%
TCTCTCTTAATTTCATGTTC+TGG - chr1.4:68575707-68575726 MS.gene21055:CDS 30.0%
TCTTACAAACTCAAATGTGT+TGG - chr1.4:68575938-68575957 MS.gene21055:intron 30.0%
TGATTGTTCTCAAATACAAC+AGG - chr1.4:68577087-68577106 MS.gene21055:CDS 30.0%
! ATAATCCAATGCTGATTGAA+GGG + chr1.4:68577050-68577069 None:intergenic 30.0%
! ATGATAGAAGATAGCATTGA+TGG - chr1.4:68576299-68576318 MS.gene21055:CDS 30.0%
! TTAAGAGCATGCATAATCAA+AGG + chr1.4:68576125-68576144 None:intergenic 30.0%
!! CTTTTGATAGTAACTGTTGA+AGG + chr1.4:68577161-68577180 None:intergenic 30.0%
AAAAACATCATTGCTAGCTG+TGG - chr1.4:68576071-68576090 MS.gene21055:CDS 35.0%
AAGACTATACAGCCAGTAAA+AGG + chr1.4:68577782-68577801 None:intergenic 35.0%
AAGATCTCTCTTCTAGACAA+AGG + chr1.4:68576587-68576606 None:intergenic 35.0%
CGAGTTACTTCTATTGAACT+TGG + chr1.4:68576443-68576462 None:intergenic 35.0%
GATGTTGTGTTTACAATAGG+AGG + chr1.4:68577734-68577753 None:intergenic 35.0%
TCTCTACTTAAACAAAAGCC+AGG - chr1.4:68575965-68575984 MS.gene21055:CDS 35.0%
TGATATCATTGGTGAAGTTG+AGG + chr1.4:68577376-68577395 None:intergenic 35.0%
TTGAGTTTAACACTGAAGGT+GGG + chr1.4:68577667-68577686 None:intergenic 35.0%
TTGCTTGAGTTTAACACTGA+AGG + chr1.4:68577671-68577690 None:intergenic 35.0%
TTGGATTATGCTTGTGGAAA+TGG - chr1.4:68577061-68577080 MS.gene21055:CDS 35.0%
! AATGCTGTCTTTTGTAGCTA+TGG - chr1.4:68577638-68577657 MS.gene21055:CDS 35.0%
! ATAACTGGTGTTGGATTTGT+AGG + chr1.4:68576671-68576690 None:intergenic 35.0%
! ATAATGGTTGGAGTATCTGA+GGG + chr1.4:68576698-68576717 None:intergenic 35.0%
! CATAATCCAATGCTGATTGA+AGG + chr1.4:68577051-68577070 None:intergenic 35.0%
! GAAGTTGAAGTTGATTGTGT+AGG + chr1.4:68577411-68577430 None:intergenic 35.0%
! TAACTGGTGTTGGATTTGTA+GGG + chr1.4:68576670-68576689 None:intergenic 35.0%
! TATAATGGTTGGAGTATCTG+AGG + chr1.4:68576699-68576718 None:intergenic 35.0%
!! CACTAGTTGAGCTTTTAACT+CGG + chr1.4:68576027-68576046 None:intergenic 35.0%
AACAACTCCAACCATTACAC+CGG - chr1.4:68576817-68576836 MS.gene21055:CDS 40.0%
AACTTCCTCAAGACTTGAAG+TGG + chr1.4:68576212-68576231 None:intergenic 40.0%
ACATCTTATCCACCTCCATT+AGG - chr1.4:68576917-68576936 MS.gene21055:CDS 40.0%
ACCACTGGAACATTTCCTAA+TGG + chr1.4:68576935-68576954 None:intergenic 40.0%
ACTGGAACATTTCCTAATGG+AGG + chr1.4:68576932-68576951 None:intergenic 40.0%
AGAGAGAAGAGAGAAACAAG+AGG + chr1.4:68575694-68575713 None:intergenic 40.0%
ATGAAGAAACTTTGCTCTGC+TGG + chr1.4:68575875-68575894 None:intergenic 40.0%
ATTGTGACAGGATTTGTGGA+AGG + chr1.4:68576728-68576747 None:intergenic 40.0%
CAGTGATCTTCACATAAGCA+AGG - chr1.4:68576163-68576182 MS.gene21055:CDS 40.0%
CATTTCCACTTCAAGTCTTG+AGG - chr1.4:68576204-68576223 MS.gene21055:intron 40.0%
CTCTTGATTGTAAGAACCAC+AGG + chr1.4:68576410-68576429 None:intergenic 40.0%
CTTGAGTTTAACACTGAAGG+TGG + chr1.4:68577668-68577687 None:intergenic 40.0%
GAAAGAACTCAAAAGGGAAG+AGG - chr1.4:68576550-68576569 MS.gene21055:CDS 40.0%
GAAGGAACTGCTATAATGGT+TGG + chr1.4:68576710-68576729 None:intergenic 40.0%
GCCGATGTTGTGTTTACAAT+AGG + chr1.4:68577737-68577756 None:intergenic 40.0%
GCTATTGTTGGAATAGATGG+TGG + chr1.4:68577462-68577481 None:intergenic 40.0%
GGAAATTGTGACAGGATTTG+TGG + chr1.4:68576732-68576751 None:intergenic 40.0%
GGAGCTATTGTTGGAATAGA+TGG + chr1.4:68577465-68577484 None:intergenic 40.0%
GTAGGGTAAACTGTGTTTGT+TGG + chr1.4:68576653-68576672 None:intergenic 40.0%
GTAGTTGAAGGAACTGTTAC+TGG + chr1.4:68576776-68576795 None:intergenic 40.0%
TAAAAGGTTTCAGAGCTCGA+GGG + chr1.4:68577766-68577785 None:intergenic 40.0%
TCACTTTCATGCAGGTTCTT+TGG - chr1.4:68575803-68575822 MS.gene21055:CDS 40.0%
TCCATTAGGAAATGTTCCAG+TGG - chr1.4:68576931-68576950 MS.gene21055:CDS 40.0%
TGAGTTTAACACTGAAGGTG+GGG + chr1.4:68577666-68577685 None:intergenic 40.0%
TGTGGAAGGAACTGCTATAA+TGG + chr1.4:68576714-68576733 None:intergenic 40.0%
TGTTGAAGGCAAAAGAAGCA+TGG + chr1.4:68577147-68577166 None:intergenic 40.0%
TTAACTCGGAAACTGCTGAA+GGG + chr1.4:68576013-68576032 None:intergenic 40.0%
TTTAACTCGGAAACTGCTGA+AGG + chr1.4:68576014-68576033 None:intergenic 40.0%
TTTCATGCAGGTTCTTTGGT+GGG - chr1.4:68575807-68575826 MS.gene21055:CDS 40.0%
TTTGTAGGGGAAATTGTGAC+AGG + chr1.4:68576740-68576759 None:intergenic 40.0%
! ATTGTTGGAATAGATGGTGG+AGG + chr1.4:68577459-68577478 None:intergenic 40.0%
! ATTGTTGGTGGAGCTATTGT+TGG + chr1.4:68577474-68577493 None:intergenic 40.0%
! CAACATCTTCAGGCACTTTT+GGG - chr1.4:68577529-68577548 MS.gene21055:CDS 40.0%
! CAGGTACAATGCCAGATTTT+GGG - chr1.4:68577699-68577718 MS.gene21055:intron 40.0%
! GGTGTTGGAGATGATATCAT+TGG + chr1.4:68577387-68577406 None:intergenic 40.0%
! GGTTTTTCCTATCATGAGAG+AGG - chr1.4:68575828-68575847 MS.gene21055:CDS 40.0%
! GTATCTGAGGGTGTAATAAC+TGG + chr1.4:68576686-68576705 None:intergenic 40.0%
! GTTGAAGTTGATTGTGTAGG+TGG + chr1.4:68577408-68577427 None:intergenic 40.0%
! TCAGCATTGGATTATGCTTG+TGG - chr1.4:68577055-68577074 MS.gene21055:CDS 40.0%
! TCAGGTACAATGCCAGATTT+TGG - chr1.4:68577698-68577717 MS.gene21055:intron 40.0%
!! GAAGGAGCATTTGTGTTTGA+AGG + chr1.4:68576977-68576996 None:intergenic 40.0%
ACAAATGCTCCTTCTGCTCA+GGG - chr1.4:68576983-68577002 MS.gene21055:CDS 45.0%
ACAACTCCTACAACAGTTCC+AGG - chr1.4:68576860-68576879 MS.gene21055:CDS 45.0%
ACCACAGGAACATCTTTGCA+AGG + chr1.4:68576395-68576414 None:intergenic 45.0%
ACTGTTGTAGGAGTTGTAGC+TGG + chr1.4:68576857-68576876 None:intergenic 45.0%
AGCTTCCCTTCAATCAGCAT+TGG - chr1.4:68577042-68577061 MS.gene21055:CDS 45.0%
AGTGTCTCCTCTCTCATGAT+AGG + chr1.4:68575838-68575857 None:intergenic 45.0%
ATCATGAGAGAGGAGACACT+TGG - chr1.4:68575838-68575857 MS.gene21055:CDS 45.0%
CATTGCAGCTGGATTTGTAG+GGG + chr1.4:68576753-68576772 None:intergenic 45.0%
CTTTCATGCAGGTTCTTTGG+TGG - chr1.4:68575806-68575825 MS.gene21055:CDS 45.0%
GAACTCAAAAGGGAAGAGGA+AGG - chr1.4:68576554-68576573 MS.gene21055:CDS 45.0%
GACGACGATGAAGATGATGA+CGG + chr1.4:68577348-68577367 None:intergenic 45.0%
GAGCTTTCGTCACAGATCAT+AGG - chr1.4:68575904-68575923 MS.gene21055:intron 45.0%
GCATTGCAGCTGGATTTGTA+GGG + chr1.4:68576754-68576773 None:intergenic 45.0%
GCATTTGTGTTTGAAGGAGG+TGG + chr1.4:68576971-68576990 None:intergenic 45.0%
GGAACATTTCCTAATGGAGG+TGG + chr1.4:68576929-68576948 None:intergenic 45.0%
GGAGGTGGATAAGATGTTAC+TGG + chr1.4:68576893-68576912 None:intergenic 45.0%
GGATAAGATGTTACTGGAGG+TGG + chr1.4:68576908-68576927 None:intergenic 45.0%
GGTGGATAAGATGTTACTGG+AGG + chr1.4:68576890-68576909 None:intergenic 45.0%
GGTGGATAAGATGTTACTGG+TGG + chr1.4:68576890-68576909 None:intergenic 45.0%
GGTGTAATGGTTGGAGTTGT+TGG + chr1.4:68576818-68576837 None:intergenic 45.0%
GTAAAAGGTTTCAGAGCTCG+AGG + chr1.4:68577767-68577786 None:intergenic 45.0%
GTAATGGTTGGAGTTGTTGG+AGG + chr1.4:68576815-68576834 None:intergenic 45.0%
GTTGATTGTGTAGGTGGTGT+TGG + chr1.4:68577402-68577421 None:intergenic 45.0%
GTTGGAGTTGTTGGAGGTAA+AGG + chr1.4:68576809-68576828 None:intergenic 45.0%
GTTGTAGCTGGATTGAAGAC+TGG + chr1.4:68576845-68576864 None:intergenic 45.0%
TCCTTGCAAAGATGTTCCTG+TGG - chr1.4:68576391-68576410 MS.gene21055:CDS 45.0%
! AAAGTGCCTGAAGATGTTGG+TGG + chr1.4:68577528-68577547 None:intergenic 45.0%
! CCAACATCTTCAGGCACTTT+TGG - chr1.4:68577528-68577547 MS.gene21055:CDS 45.0%
! GAGGGTGTAATAACTGGTGT+TGG + chr1.4:68576680-68576699 None:intergenic 45.0%
! GCATGGTTCTGCAAAGTAAC+AGG + chr1.4:68577130-68577149 None:intergenic 45.0%
! GGAGCATTTGTGTTTGAAGG+AGG + chr1.4:68576974-68576993 None:intergenic 45.0%
!! CCAAAAGTGCCTGAAGATGT+TGG + chr1.4:68577531-68577550 None:intergenic 45.0%
!! GGAGGTGTTGTTAGATTTGG+TGG + chr1.4:68577441-68577460 None:intergenic 45.0%
!! GGTGGAGGTGTTGTTAGATT+TGG + chr1.4:68577444-68577463 None:intergenic 45.0%
!! GGTGTTGTTAGATTTGGTGG+TGG + chr1.4:68577438-68577457 None:intergenic 45.0%
!!! CGAGCTCTGAAACCTTTTAC+TGG - chr1.4:68577767-68577786 MS.gene21055:intron 45.0%
AACTCAAGCAACCCAGCTTC+AGG - chr1.4:68577680-68577699 MS.gene21055:intron 50.0%
ACAGCTCCACCAACATCTTC+AGG - chr1.4:68577519-68577538 MS.gene21055:CDS 50.0%
ACTGTTACTGGCATTGCAGC+TGG + chr1.4:68576764-68576783 None:intergenic 50.0%
CACAAATGCTCCTTCTGCTC+AGG - chr1.4:68576982-68577001 MS.gene21055:CDS 50.0%
CAGAAGCAGCAAGATTAGCG+CGG + chr1.4:68576367-68576386 None:intergenic 50.0%
CCAACAAGTTGCGACTTTGG+AGG - chr1.4:68577190-68577209 MS.gene21055:CDS 50.0%
CCTCCAAAGTCGCAACTTGT+TGG + chr1.4:68577193-68577212 None:intergenic 50.0%
CTGAGGCTTGCAGTGACAAA+TGG + chr1.4:68577812-68577831 None:intergenic 50.0%
CTGGCATTGTACCTGAAGCT+GGG + chr1.4:68577694-68577713 None:intergenic 50.0%
GAGGCAGAAAGAAGCTTCCT+TGG + chr1.4:68575675-68575694 None:intergenic 50.0%
GCTCCAACAAGTTGCGACTT+TGG - chr1.4:68577187-68577206 MS.gene21055:CDS 50.0%
GCTGATTGAAGGGAAGCTTG+TGG + chr1.4:68577040-68577059 None:intergenic 50.0%
GGAACAGCAGGTGTAGTTGA+AGG + chr1.4:68576788-68576807 None:intergenic 50.0%
GGAGCTGTTGACATTGTTGG+TGG + chr1.4:68577486-68577505 None:intergenic 50.0%
GGAGCTGTTGGAATTGATGG+TGG + chr1.4:68577507-68577526 None:intergenic 50.0%
GGCATTGCAGCTGGATTTGT+AGG + chr1.4:68576755-68576774 None:intergenic 50.0%
GGGCTATCAGACCCAAAATC+TGG + chr1.4:68577713-68577732 None:intergenic 50.0%
GGTGGAGCTGTTGACATTGT+TGG + chr1.4:68577489-68577508 None:intergenic 50.0%
GGTGGAGCTGTTGGAATTGA+TGG + chr1.4:68577510-68577529 None:intergenic 50.0%
GTTGGAGGTAAAGGAACAGC+AGG + chr1.4:68576800-68576819 None:intergenic 50.0%
TCTGGCATTGTACCTGAAGC+TGG + chr1.4:68577695-68577714 None:intergenic 50.0%
TTGAAGGAGGTGGCTGTTGA+GGG + chr1.4:68576961-68576980 None:intergenic 50.0%
TTTGAAGGAGGTGGCTGTTG+AGG + chr1.4:68576962-68576981 None:intergenic 50.0%
! AGTTGGTGTGTGGCGAAAAC+AGG - chr1.4:68577010-68577029 MS.gene21055:CDS 50.0%
! GAAGATGTTGGTGGAGCTGT+TGG + chr1.4:68577519-68577538 None:intergenic 50.0%
!! AAGTCGCAACTTGTTGGAGC+TGG + chr1.4:68577187-68577206 None:intergenic 50.0%
!! GGCACTTTTGGGTAAGTGAG+AGG - chr1.4:68577540-68577559 MS.gene21055:CDS 50.0%
CAACTCTGTCCCTGAGCAGA+AGG + chr1.4:68576995-68577014 None:intergenic 55.0%
CTTCTGCTCAGGGACAGAGT+TGG - chr1.4:68576993-68577012 MS.gene21055:CDS 55.0%
GCTGGATTGAAGACTGGTGC+CGG + chr1.4:68576839-68576858 None:intergenic 55.0%
GGCTGTTGAGGGTTTACCAC+TGG + chr1.4:68576950-68576969 None:intergenic 55.0%
GGTGCACCTGGAACTGTTGT+AGG + chr1.4:68576869-68576888 None:intergenic 55.0%
! GATGTTACTGGTGGTGCACC+TGG + chr1.4:68576881-68576900 None:intergenic 55.0%
!! ACTGGTGCCGGTGTAATGGT+TGG + chr1.4:68576827-68576846 None:intergenic 55.0%
!! GAAGACTGGTGCCGGTGTAA+TGG + chr1.4:68576831-68576850 None:intergenic 55.0%
!! GGAGGCGCTGCTATGTTAGT+TGG - chr1.4:68577208-68577227 MS.gene21055:CDS 55.0%
!! TCAGGGACAGAGTTGGTGTG+TGG - chr1.4:68577000-68577019 MS.gene21055:CDS 55.0%
! TTTCAGAGCTCGAGGGTGCG+AGG + chr1.4:68577759-68577778 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 68575668 68577844 68575668 ID=MS.gene21055
chr1.4 mRNA 68575668 68577844 68575668 ID=MS.gene21055.t1;Parent=MS.gene21055
chr1.4 exon 68577784 68577844 68577784 ID=MS.gene21055.t1.exon1;Parent=MS.gene21055.t1
chr1.4 CDS 68577784 68577844 68577784 ID=cds.MS.gene21055.t1;Parent=MS.gene21055.t1
chr1.4 exon 68576271 68577695 68576271 ID=MS.gene21055.t1.exon2;Parent=MS.gene21055.t1
chr1.4 CDS 68576271 68577695 68576271 ID=cds.MS.gene21055.t1;Parent=MS.gene21055.t1
chr1.4 exon 68575962 68576187 68575962 ID=MS.gene21055.t1.exon3;Parent=MS.gene21055.t1
chr1.4 CDS 68575962 68576187 68575962 ID=cds.MS.gene21055.t1;Parent=MS.gene21055.t1
chr1.4 exon 68575668 68575857 68575668 ID=MS.gene21055.t1.exon4;Parent=MS.gene21055.t1
chr1.4 CDS 68575668 68575857 68575668 ID=cds.MS.gene21055.t1;Parent=MS.gene21055.t1
Gene Sequence

>MS.gene21055

ATGGCCAAGGAAGCTTCTTTCTGCCTCTTGTTTCTCTCTTCTCTCTTAATTTCATGTTCTGGTAAGTACTTTACACTGATAAATGATAATAACACATAGTACTCATTTTTGTGCAGATTCTAATCGTATATTATTTCACTTTCATGCAGGTTCTTTGGTGGGTTTTTCCTATCATGAGAGAGGAGACACTTGGACATCATTACTCCAGCAGAGCAAAGTTTCTTCATCTCAGATTCGAGCTTTCGTCACAGATCATAGGATTTTGAGCACTCTTACAAACTCAAATGTGTTGGTTGATCTCTACTTAAACAAAAGCCAGGTTGAAAAATTCATTACTTCAAACCCTTCAGCAGTTTCCGAGTTAAAAGCTCAACTAGTGAGCTTTCTTCCACATTTAAACATCAAAAACATCATTGCTAGCTGTGGAAGTGAGTGCTTAGTACAAAATGAGATACCTTTGATTATGCATGCTCTTAAATCAATTCATTCAATTCTCAGTGATCTTCACATAAGCAAGGAAGTAAAAATCTCGGTCGCATTTCCACTTCAAGTCTTGAGGAAGTTGAATGCATCACAAGAACACGAAATTCGTAGATTACTTTCATTTATAAAGGAAACGAAATCGTTTGTAATGATAGAAGATAGCATTGATGGAGAATTAAGTGTGAATGATCATTTTGTTCAAACAATTATCAACCGCGCTAATCTTGCTGCTTCTGTTCTTCCTTGCAAAGATGTTCCTGTGGTTCTTACAATCAAGAGTTCAGTTATACCAAGTTCAATAGAAGTAACTCGATTTAGTAAAAGAGTATCCAAATATTTAGAAGCAAAAAGAATAGCTGCATTATATGTAGAACTGCATACAACAGAAGATTCTTCAATGAAAGAACTCAAAAGGGAAGAGGAAGGTATGTTTCCTTTGTCTAGAAGAGAGATCTTAAGCAAATTCCACAGAAGAAATATTCTAGATAACACAAACAGTCCAACAAACACAGTTTACCCTACAAATCCAACACCAGTTATTACACCCTCAGATACTCCAACCATTATAGCAGTTCCTTCCACAAATCCTGTCACAATTTCCCCTACAAATCCAGCTGCAATGCCAGTAACAGTTCCTTCAACTACACCTGCTGTTCCTTTACCTCCAACAACTCCAACCATTACACCGGCACCAGTCTTCAATCCAGCTACAACTCCTACAACAGTTCCAGGTGCACCACCAGTAACATCTTATCCACCTCCAGTAACATCTTATCCACCTCCATTAGGAAATGTTCCAGTGGTAAACCCTCAACAGCCACCTCCTTCAAACACAAATGCTCCTTCTGCTCAGGGACAGAGTTGGTGTGTGGCGAAAACAGGTGCTCCACAAGCTTCCCTTCAATCAGCATTGGATTATGCTTGTGGAAATGGTGCTGATTGTTCTCAAATACAACAGGGTGCAAGTTGTTACAGTCCTGTTACTTTGCAGAACCATGCTTCTTTTGCCTTCAACAGTTACTATCAAAAGAAGCCAGCTCCAACAAGTTGCGACTTTGGAGGCGCTGCTATGTTAGTTGGCTCAAATCCAAGTAAGATTTTAACATTTAAATAACCCTTGTTTTTTCTTTCTTTCAATCTCATGCTTCAAAATATTGATGATCATTTTTAATAGGTTCTGGTTCTTGCATTTATCCGTCATCATCTTCATCGTCGTCGTCATCCTCAACTTCACCAATGATATCATCTCCAACACCACCTACACAATCAACTTCAACTTCAATACCACCACCAAATCTAACAACACCTCCACCATCTATTCCAACAATAGCTCCACCAACAATGTCAACAGCTCCACCATCAATTCCAACAGCTCCACCAACATCTTCAGGCACTTTTGGGTAAGTGAGAGGCAAGTTTTTCAGTGACTTCAATAATTATATTCTGTGATACCAATAAGCATGTACTAATCATGGTTCTGTATAAACAATGCTGTCTTTTGTAGCTATGGTACCCCACCTTCAGTGTTAAACTCAAGCAACCCAGCTTCAGGTACAATGCCAGATTTTGGGTCTGATAGCCCTCCTATTGTAAACACAACATCGGCCTCGCACCCTCGAGCTCTGAAACCTTTTACTGGCTGTATAGTCTTGATGATTCCATTTGTCACTGCAAGCCTCAGCATGCACCTATAG
Protein sequence

>MS.gene21055.t1

MAKEASFCLLFLSSLLISCSGSLVGFSYHERGDTWTSLLQQSKVSSSQIRAFVTDHRILSTLTNSNVLVDLYLNKSQVEKFITSNPSAVSELKAQLVSFLPHLNIKNIIASCGSECLVQNEIPLIMHALKSIHSILSDLHISKEVKISVAFPLQVLRKLNASQEHEIRRLLSFIKETKSFVMIEDSIDGELSVNDHFVQTIINRANLAASVLPCKDVPVVLTIKSSVIPSSIEVTRFSKRVSKYLEAKRIAALYVELHTTEDSSMKELKREEEGMFPLSRREILSKFHRRNILDNTNSPTNTVYPTNPTPVITPSDTPTIIAVPSTNPVTISPTNPAAMPVTVPSTTPAVPLPPTTPTITPAPVFNPATTPTTVPGAPPVTSYPPPVTSYPPPLGNVPVVNPQQPPPSNTNAPSAQGQSWCVAKTGAPQASLQSALDYACGNGADCSQIQQGASCYSPVTLQNHASFAFNSYYQKKPAPTSCDFGGAAMLVGSNPSSGSCIYPSSSSSSSSSSTSPMISSPTPPTQSTSTSIPPPNLTTPPPSIPTIAPPTMSTAPPSIPTAPPTSSGTFGYGTPPSVLNSSNPASGTMPDFGSDSPPIVNTTSASHPRALKPFTGCIVLMIPFVTASLSMHL