AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21415

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21415.t1 MTR_1g090737 97.368 570 15 0 1 570 1 570 0.0 1137
MS.gene21415.t1 MTR_4g057730 43.716 549 294 7 24 566 3 542 1.20e-152 449
MS.gene21415.t1 MTR_1g026310 45.652 552 279 9 22 559 9 553 5.11e-152 448
MS.gene21415.t1 MTR_2g095510 40.585 547 313 6 23 566 2 539 1.20e-144 428
MS.gene21415.t1 MTR_5g027480 45.037 544 275 12 25 558 18 547 2.87e-142 423
MS.gene21415.t1 MTR_8g039720 43.937 569 280 15 12 559 2 552 8.90e-140 417
MS.gene21415.t1 MTR_5g027500 44.123 553 275 11 27 558 21 560 4.70e-138 412
MS.gene21415.t1 MTR_4g077900 47.648 489 232 10 95 570 1 478 1.06e-135 403
MS.gene21415.t1 MTR_3g088870 45.219 502 255 7 67 559 61 551 7.75e-135 404
MS.gene21415.t1 MTR_1g063110 43.156 526 283 10 45 559 21 541 2.21e-132 397
MS.gene21415.t1 MTR_3g088880 43.863 497 266 8 67 559 62 549 1.27e-127 385
MS.gene21415.t1 MTR_1g063120 41.774 541 292 10 46 570 22 555 9.63e-126 380
MS.gene21415.t1 MTR_4g057730 42.469 478 260 7 24 495 3 471 5.49e-121 365
MS.gene21415.t1 MTR_1g073180 40.741 540 306 8 21 559 1 527 1.09e-115 354
MS.gene21415.t1 MTR_3g460770 38.031 518 306 6 46 556 22 531 4.49e-114 350
MS.gene21415.t1 MTR_2g105570 39.575 518 283 9 59 558 36 541 2.60e-111 343
MS.gene21415.t1 MTR_4g128337 39.179 536 301 9 45 567 26 549 4.87e-111 342
MS.gene21415.t1 MTR_4g057730 41.886 456 250 7 24 473 3 449 1.32e-109 335
MS.gene21415.t1 MTR_5g007640 39.448 507 285 7 66 558 81 579 1.71e-103 324
MS.gene21415.t1 MTR_2g095510 38.411 453 267 6 24 473 3 446 1.49e-102 317
MS.gene21415.t1 MTR_1g073180 39.655 522 299 9 22 542 24 530 2.50e-102 319
MS.gene21415.t1 MTR_4g005750 39.558 498 289 6 70 558 40 534 3.80e-102 319
MS.gene21415.t1 MTR_1g026430 39.500 400 221 9 22 407 9 401 2.02e-76 248
MS.gene21415.t1 MTR_1g026470 39.500 400 221 9 22 407 9 401 6.05e-76 247
MS.gene21415.t1 MTR_1569s0010 29.961 514 314 13 83 558 22 527 2.75e-53 190
MS.gene21415.t1 MTR_3g035130 29.039 489 317 14 83 556 32 505 2.75e-37 145
MS.gene21415.t1 MTR_8g074040 25.631 515 332 14 84 563 83 581 2.09e-35 140
MS.gene21415.t1 MTR_5g076240 27.237 514 315 16 84 555 41 537 6.28e-35 139
MS.gene21415.t1 MTR_2g044180 27.979 386 240 11 209 570 101 472 1.51e-34 136
MS.gene21415.t1 MTR_2g044180 28.232 379 234 11 209 563 192 556 4.19e-34 136
MS.gene21415.t1 MTR_0803s0020 26.844 488 321 13 85 552 263 734 1.50e-33 137
MS.gene21415.t1 MTR_3g064420 25.660 530 335 16 82 568 43 556 2.45e-33 134
MS.gene21415.t1 MTR_5g008800 26.488 521 334 15 82 570 39 542 6.64e-33 133
MS.gene21415.t1 MTR_4g121600 27.394 376 241 12 209 563 192 556 3.63e-30 125
MS.gene21415.t1 MTR_8g074040 25.159 473 304 13 126 563 6 463 4.41e-30 124
MS.gene21415.t1 MTR_2g030240 28.200 461 269 15 80 498 25 465 4.58e-29 121
MS.gene21415.t1 MTR_5g008820 25.869 518 335 19 84 570 41 540 6.29e-29 121
MS.gene21415.t1 MTR_5g076220 25.407 492 318 13 105 558 61 541 8.31e-29 120
MS.gene21415.t1 MTR_8g074120 26.016 492 304 14 111 563 20 490 1.16e-28 120
MS.gene21415.t1 MTR_4g013210 28.158 380 229 11 212 558 70 438 3.28e-28 117
MS.gene21415.t1 MTR_1g026350 41.447 152 80 3 22 170 9 154 3.68e-28 111
MS.gene21415.t1 MTR_3g035130 29.358 436 278 14 83 503 32 452 9.28e-28 117
MS.gene21415.t1 MTR_2g030240 28.852 305 185 8 216 498 77 371 3.21e-26 112
MS.gene21415.t1 MTR_4g011790 26.065 399 243 11 196 558 209 591 9.26e-26 112
MS.gene21415.t1 MTR_0161s0050 25.349 501 309 19 105 556 61 545 1.24e-25 111
MS.gene21415.t1 MTR_4g011790 26.110 383 231 11 207 553 220 586 2.32e-24 107
MS.gene21415.t1 MTR_4g013210 27.989 368 204 12 212 558 70 397 1.81e-23 103
MS.gene21415.t1 MTR_4g011790 25.490 408 243 12 196 558 209 600 1.90e-23 105
MS.gene21415.t1 MTR_5g076200 23.838 495 322 15 105 556 61 543 3.92e-23 103
MS.gene21415.t1 MTR_4g013210 27.989 368 204 12 212 558 222 549 1.03e-22 102
MS.gene21415.t1 MTR_4g057755 50.562 89 43 1 409 496 32 120 7.36e-22 91.7
MS.gene21415.t1 MTR_5g023730 24.046 524 324 20 78 555 38 533 1.91e-21 98.6
MS.gene21415.t1 MTR_4g013210 26.966 356 216 10 212 534 222 566 2.67e-21 98.2
MS.gene21415.t1 MTR_1054s0020 25.750 400 271 12 171 565 391 769 5.33e-19 91.7
MS.gene21415.t1 MTR_7g084850 22.822 574 364 19 53 566 170 724 8.66e-19 90.9
MS.gene21415.t1 MTR_7g109830 28.571 350 171 14 204 493 217 547 5.69e-18 88.2
MS.gene21415.t1 MTR_1g086600 27.933 358 176 13 212 498 243 589 7.29e-18 87.8
MS.gene21415.t1 MTR_8g099420 26.389 360 187 15 186 484 199 541 1.61e-17 86.7
MS.gene21415.t1 MTR_3g088890 52.381 63 30 0 468 530 10 72 6.27e-15 70.9
MS.gene21415.t1 MTR_2g098490 27.326 344 200 14 194 499 633 964 5.64e-14 75.9
MS.gene21415.t1 MTR_3g084470 28.571 252 130 7 355 566 469 710 1.00e-13 74.7
MS.gene21415.t1 MTR_8g035620 22.885 520 345 12 82 559 117 622 2.74e-13 73.6
MS.gene21415.t1 MTR_3g084450 32.609 184 94 5 355 518 265 438 4.92e-13 72.0
MS.gene21415.t1 MTR_3g084450 32.609 184 94 5 355 518 473 646 7.18e-13 72.0
MS.gene21415.t1 MTR_8g083170 29.358 218 127 7 334 536 459 664 1.36e-12 70.9
MS.gene21415.t1 MTR_4g013210 27.208 283 155 10 212 483 222 464 2.64e-11 66.6
MS.gene21415.t1 MTR_1g030290 30.864 162 100 6 335 488 426 583 6.22e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21415.t1 AT1G20510 45.620 548 281 8 18 559 1 537 1.28e-156 459
MS.gene21415.t1 AT1G20500 43.750 544 283 9 28 559 11 543 1.12e-147 437
MS.gene21415.t1 AT4G05160 42.831 551 298 6 24 566 3 544 4.30e-147 435
MS.gene21415.t1 AT4G19010 43.462 543 283 10 27 558 25 554 1.75e-140 419
MS.gene21415.t1 AT5G38120 42.647 544 288 9 28 559 12 543 1.86e-138 413
MS.gene21415.t1 AT1G20480 43.013 551 285 12 22 558 22 557 1.33e-136 409
MS.gene21415.t1 AT5G63380 42.468 551 291 11 22 558 12 550 6.40e-135 404
MS.gene21415.t1 AT1G20510 43.595 484 256 8 18 495 1 473 1.13e-123 372
MS.gene21415.t1 AT5G38120 41.165 515 279 9 28 530 12 514 6.06e-121 367
MS.gene21415.t1 AT1G20510 41.991 462 251 8 18 473 1 451 1.19e-110 338
MS.gene21415.t1 AT1G65060 41.513 489 275 5 78 558 71 556 3.08e-109 338
MS.gene21415.t1 AT5G38120 40.417 480 262 9 28 495 12 479 1.99e-107 331
MS.gene21415.t1 AT5G38120 40.417 480 262 9 28 495 12 479 1.99e-107 331
MS.gene21415.t1 AT3G21240 39.679 499 270 8 79 558 60 546 7.96e-107 332
MS.gene21415.t1 AT1G62940 40.927 496 280 6 71 558 42 532 5.46e-105 327
MS.gene21415.t1 AT3G21230 40.354 508 286 6 65 558 56 560 4.71e-104 325
MS.gene21415.t1 AT1G51680 39.286 504 288 7 70 558 53 553 3.83e-98 309
MS.gene21415.t1 AT3G21230 39.583 480 273 6 65 530 56 532 8.66e-92 293
MS.gene21415.t1 AT1G51680 38.445 476 275 7 70 530 53 525 6.10e-86 277
MS.gene21415.t1 AT1G20490 37.355 431 243 10 28 443 11 429 1.47e-80 260
MS.gene21415.t1 AT1G20490 37.355 431 243 10 28 443 11 429 1.47e-80 260
MS.gene21415.t1 AT3G21240 38.161 435 238 8 79 494 60 482 4.63e-80 260
MS.gene21415.t1 AT3G21240 38.161 435 238 8 79 494 60 482 4.63e-80 260
MS.gene21415.t1 AT3G21230 39.101 445 254 6 64 494 55 496 1.54e-79 259
MS.gene21415.t1 AT1G65060 38.916 406 237 5 78 475 71 473 1.02e-74 246
MS.gene21415.t1 AT1G51680 37.727 440 256 7 70 494 53 489 1.19e-72 241
MS.gene21415.t1 AT3G21230 34.853 373 226 6 65 423 56 425 2.18e-44 164
MS.gene21415.t1 AT3G48990 29.857 489 315 12 82 556 30 504 1.37e-40 154
MS.gene21415.t1 AT1G65880 27.920 548 323 17 82 569 32 567 1.72e-39 152
MS.gene21415.t1 AT1G30520 27.531 563 325 18 70 568 14 557 1.25e-38 149
MS.gene21415.t1 AT1G30520 27.531 563 325 18 70 568 14 557 1.25e-38 149
MS.gene21415.t1 AT1G21540 27.799 518 318 17 79 555 37 539 2.97e-37 145
MS.gene21415.t1 AT1G65890 27.032 529 322 16 82 558 32 548 7.70e-37 145
MS.gene21415.t1 AT1G77240 26.499 517 326 17 77 555 34 534 1.93e-34 137
MS.gene21415.t1 AT1G20560 26.437 522 333 15 84 570 43 548 8.77e-34 135
MS.gene21415.t1 AT1G66120 27.533 523 320 18 82 558 32 541 1.56e-33 135
MS.gene21415.t1 AT1G21530 25.769 520 325 17 78 555 38 538 2.10e-33 134
MS.gene21415.t1 AT1G21530 26.004 523 318 18 78 555 38 536 5.05e-33 133
MS.gene21415.t1 AT5G16370 25.294 510 336 15 79 555 37 534 3.48e-32 130
MS.gene21415.t1 AT1G75960 25.686 510 330 13 84 558 42 537 6.23e-30 124
MS.gene21415.t1 AT1G20560 26.400 375 252 9 209 570 107 470 9.78e-30 122
MS.gene21415.t1 AT1G30520 27.292 469 271 16 70 490 14 460 1.05e-29 122
MS.gene21415.t1 AT1G30520 27.292 469 271 16 70 490 14 460 1.05e-29 122
MS.gene21415.t1 AT5G16340 24.361 509 342 14 79 555 37 534 4.21e-29 122
MS.gene21415.t1 AT3G16910 28.042 378 236 14 209 563 195 559 1.65e-28 120
MS.gene21415.t1 AT3G16170 26.649 379 233 11 212 558 235 600 2.44e-27 117
MS.gene21415.t1 AT2G17650 25.000 520 325 17 84 555 88 590 2.45e-24 107
MS.gene21415.t1 AT1G76290 23.917 531 346 17 73 563 23 535 2.34e-23 104
MS.gene21415.t1 AT1G68270 25.281 534 295 20 82 558 32 518 2.49e-23 104
MS.gene21415.t1 AT5G27600 27.044 318 174 11 212 481 262 569 4.99e-19 91.7
MS.gene21415.t1 AT3G05970 27.586 319 171 10 212 481 262 569 8.81e-18 87.4
MS.gene21415.t1 AT5G36880 23.475 541 352 17 76 566 141 669 1.29e-17 87.0
MS.gene21415.t1 AT5G36880 23.475 541 352 17 76 566 141 669 1.29e-17 87.0
MS.gene21415.t1 AT5G36880 23.475 541 352 17 76 566 141 669 1.29e-17 87.0
MS.gene21415.t1 AT5G36880 23.475 541 352 17 76 566 141 669 1.29e-17 87.0
MS.gene21415.t1 AT5G36880 23.475 541 352 17 76 566 191 719 1.33e-17 87.0
MS.gene21415.t1 AT1G49430 26.479 355 191 14 196 493 210 551 1.84e-17 86.7
MS.gene21415.t1 AT2G47240 27.679 336 180 13 212 493 221 547 3.21e-16 82.4
MS.gene21415.t1 AT2G47240 27.679 336 180 13 212 493 221 547 3.21e-16 82.4
MS.gene21415.t1 AT2G47240 27.679 336 180 13 212 493 221 547 3.21e-16 82.4
MS.gene21415.t1 AT2G47240 27.679 336 180 13 212 493 162 488 3.53e-16 82.4
MS.gene21415.t1 AT1G77590 24.277 346 193 11 211 493 99 438 1.60e-15 80.1
MS.gene21415.t1 AT2G04350 24.709 344 178 14 210 484 273 604 4.08e-15 79.3
MS.gene21415.t1 AT2G04350 24.709 344 178 14 210 484 273 604 4.08e-15 79.3
MS.gene21415.t1 AT5G35930 23.495 515 335 16 84 553 59 559 1.56e-11 68.2

Find 128 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCCAATGTTTCATGTCTA+TGG 0.176321 1.4:+71968288 MS.gene21415:CDS
ACCCTTCTCACTTTCTTATA+TGG 0.212821 1.4:-71969695 None:intergenic
ATCAGCTTTCCACATGCTTC+TGG 0.219682 1.4:-71968654 None:intergenic
GGACTGATGTTCTTTGGATT+TGG 0.232726 1.4:+71968309 MS.gene21415:CDS
GAATGGTAAATTCCTGTTCT+TGG 0.248189 1.4:-71967592 None:intergenic
GTCTATGGACTGATGTTCTT+TGG 0.250992 1.4:+71968303 MS.gene21415:CDS
GTTGAGTACAAGATTGAGTT+TGG 0.273727 1.4:-71967853 None:intergenic
TAACTGCTGCATCAACAATA+AGG 0.276654 1.4:-71969123 None:intergenic
CCAGCAGAGCAGTTTCATTA+TGG 0.284701 1.4:-71968220 None:intergenic
ACCAATTTGTGTAACTCTTC+TGG 0.296211 1.4:-71967994 None:intergenic
GACTGATGTTCTTTGGATTT+GGG 0.298826 1.4:+71968310 MS.gene21415:CDS
CTTTGTTGGGATTTCATGTT+TGG 0.301287 1.4:-71967632 None:intergenic
CTCTGTCTCCATTTGATAAC+AGG 0.330041 1.4:+71967557 MS.gene21415:CDS
CATGTCATTCAATTTGTTGC+AGG 0.341335 1.4:+71969304 MS.gene21415:CDS
AATCAAGCACAAAGGGTATC+AGG 0.343261 1.4:+71968902 MS.gene21415:CDS
GCTGCTATACTTTACTCTTC+AGG 0.349123 1.4:+71968147 MS.gene21415:CDS
AAATGTCAGGAGAGGAGATT+TGG 0.351734 1.4:+71967506 None:intergenic
AAAGTGATAGACATTGAAAC+AGG 0.356914 1.4:+71968693 MS.gene21415:CDS
CTACATAGTTGAACGAATAA+AGG 0.367598 1.4:+71968875 MS.gene21415:CDS
AACTGCTGCATCAACAATAA+GGG 0.374049 1.4:-71969122 None:intergenic
TCAGGTAGCTCCTGCAGAAC+TGG 0.384556 1.4:+71969078 MS.gene21415:intron
ACCAGAAGAGTTACACAAAT+TGG 0.386896 1.4:+71967993 MS.gene21415:CDS
TCAGTTGAAGAGTTAATAGA+AGG 0.392562 1.4:+71968072 MS.gene21415:CDS
GCGAAGCAAGTACATGATTC+AGG 0.393921 1.4:+71967949 MS.gene21415:CDS
AGGGTTGAAGATGAAGAAAC+TGG 0.395025 1.4:+71969226 MS.gene21415:intron
CACCTGAGCCAACCCTTCTT+AGG 0.395579 1.4:-71968494 None:intergenic
CTTTACTCTTCAGGGACTAC+TGG 0.405377 1.4:+71968156 MS.gene21415:CDS
TATTTCCTTGGGTTGAACTA+AGG 0.410515 1.4:+71968559 MS.gene21415:CDS
GTTCAGAAAGCTATTTCCTT+GGG 0.412932 1.4:+71968548 MS.gene21415:CDS
TGTTGAGATCTGGCTTTGTT+GGG 0.419272 1.4:-71967645 None:intergenic
CTGCTATACTTTACTCTTCA+GGG 0.434338 1.4:+71968148 MS.gene21415:CDS
GTTGAACTAAGGGCGGGTTA+TGG 0.435071 1.4:+71968570 MS.gene21415:CDS
CCATAATGAAACTGCTCTGC+TGG 0.435438 1.4:+71968220 MS.gene21415:CDS
GTTCCTACAATTCTCACCTC+TGG 0.439013 1.4:+71968027 MS.gene21415:CDS
GTGTTGAGATCTGGCTTTGT+TGG 0.439500 1.4:-71967646 None:intergenic
AGCGGATACGATCCAAGAAC+AGG 0.444190 1.4:+71967580 MS.gene21415:CDS
CATTCAATTTGTTGCAGGCC+AGG 0.446682 1.4:+71969309 MS.gene21415:CDS
GGATTTGGGTTGTTGTGTGT+TGG 0.451406 1.4:+71968324 MS.gene21415:CDS
TTTCAAGCTTAACAAGAGAA+TGG 0.452313 1.4:-71967609 None:intergenic
TTCACCAATGAGTGGATAAC+TGG 0.452438 1.4:-71968438 None:intergenic
GCTATTATGAAAGAATATCT+AGG 0.453902 1.4:+71968765 MS.gene21415:CDS
GTGATTTGTCTAGCCTAAGA+AGG 0.457731 1.4:+71968481 MS.gene21415:CDS
AGCACAGATTCCAGTTCTGC+AGG 0.460066 1.4:-71969088 None:intergenic
TGGATAACTGGTGGCACTGC+TGG 0.462633 1.4:-71968426 None:intergenic
AGAGTTGCAGTGTTGAGATC+TGG 0.462977 1.4:-71967655 None:intergenic
CTCACCTCTGGGACATCTGA+TGG 0.463000 1.4:+71968039 MS.gene21415:CDS
GTCCCAGAGGTGAGAATTGT+AGG 0.467301 1.4:-71968030 None:intergenic
TTACACAAATTGGTCCCAAC+TGG 0.471916 1.4:+71968003 MS.gene21415:CDS
GCATATGTGGTGAGGGCAGC+TGG 0.475126 1.4:+71969262 MS.gene21415:CDS
CAACAATTGATTCAGAAGGT+TGG 0.475182 1.4:+71968808 MS.gene21415:CDS
TGGTTGAGGACAGGTGATCT+TGG 0.485306 1.4:+71968828 MS.gene21415:CDS
TATCCGCTCCTGTTATCAAA+TGG 0.487399 1.4:-71967565 None:intergenic
GACATGAAACATTGGAATGA+AGG 0.489639 1.4:-71968283 None:intergenic
AGTTCAGAAAGCTATTTCCT+TGG 0.490058 1.4:+71968547 MS.gene21415:CDS
AGGTGCTGCACCTTTGAGTA+AGG 0.492711 1.4:+71968512 MS.gene21415:CDS
ATTTCCTTGGGTTGAACTAA+GGG 0.492805 1.4:+71968560 MS.gene21415:CDS
CCTTCCTTGAGTGGAGGCAA+AGG 0.493651 1.4:-71968720 None:intergenic
CTTGGTTATATCGATGAGAA+TGG 0.496399 1.4:+71968846 MS.gene21415:CDS
CATGGACTTGAGATTAGAAA+AGG 0.499367 1.4:+71967808 MS.gene21415:CDS
TTCACCAGATTCTTCTTCAA+CGG 0.499514 1.4:-71967533 None:intergenic
ATGGACTAACAGAAAGTTGT+GGG 0.505501 1.4:+71968589 MS.gene21415:CDS
AGCCTAAGAAGGGTTGGCTC+AGG 0.509742 1.4:+71968492 MS.gene21415:CDS
AGGAGCTAATCAAGCACAAA+GGG 0.511589 1.4:+71968895 MS.gene21415:CDS
TGACTTAGTATTGAGAGGGT+TGG 0.511924 1.4:-71967920 None:intergenic
CACTCAAGGAAGGAGAGTTA+TGG 0.515549 1.4:+71968730 MS.gene21415:CDS
TCATCCGTTGAAGAAGAATC+TGG 0.516179 1.4:+71967529 MS.gene21415:CDS
GCCACCAGTTATCCACTCAT+TGG 0.516190 1.4:+71968434 MS.gene21415:CDS
TGCTTAGCTGTGTTATCAGT+TGG 0.518737 1.4:+71967880 MS.gene21415:CDS
GTGAAACATGCTAGCAAAGA+TGG 0.522574 1.4:+71968456 MS.gene21415:CDS
TTGATTCAGAAGGTTGGTTG+AGG 0.524384 1.4:+71968814 MS.gene21415:CDS
TATCAGCAAGATCTCCTTGT+GGG 0.530117 1.4:-71967693 None:intergenic
TCAGAAGGTTGGTTGAGGAC+AGG 0.530736 1.4:+71968819 MS.gene21415:CDS
TGATAACACAGCTAAGCATA+TGG 0.534053 1.4:-71967875 None:intergenic
GAAGCCTTTGCCTCCACTCA+AGG 0.535824 1.4:+71968716 MS.gene21415:CDS
TTCCTACAATTCTCACCTCT+GGG 0.539856 1.4:+71968028 MS.gene21415:CDS
TTTCTGACTTAGTATTGAGA+GGG 0.544569 1.4:-71967924 None:intergenic
ACTAATCATAGTGTAACTTA+CGG 0.545558 1.4:+71967742 MS.gene21415:CDS
CATGGTACAAAGCTGTTGCA+AGG 0.547277 1.4:-71967789 None:intergenic
CCTTGGGTTGAACTAAGGGC+GGG 0.553742 1.4:+71968564 MS.gene21415:CDS
TCCTTGGGTTGAACTAAGGG+CGG 0.554693 1.4:+71968563 MS.gene21415:CDS
AGAATTGTAGGAACTCCAGT+TGG 0.555282 1.4:-71968018 None:intergenic
AGTATACCGAGGTCAGCTGC+TGG 0.555861 1.4:+71969731 MS.gene21415:CDS
CATAACTCTCCTTCCTTGAG+TGG 0.558671 1.4:-71968729 None:intergenic
TTTGAGTAAGGAAATGTCAC+TGG 0.559342 1.4:+71968524 MS.gene21415:CDS
ACCAATGGCATATGTGGTGA+GGG 0.561041 1.4:+71969255 MS.gene21415:CDS
GCCCTCACCACATATGCCAT+TGG 0.565672 1.4:-71969256 None:intergenic
TTGTCTAGCCTAAGAAGGGT+TGG 0.570054 1.4:+71968486 MS.gene21415:CDS
GAATTGTAGGAACTCCAGTT+GGG 0.571691 1.4:-71968017 None:intergenic
AGAATATCTAGGAAATATTG+AGG 0.571721 1.4:+71968776 MS.gene21415:CDS
CTCACAACTTAGAAACAAGC+TGG 0.574328 1.4:-71969793 None:intergenic
ATTTCTGACTTAGTATTGAG+AGG 0.580354 1.4:-71967925 None:intergenic
CCTTTGCCTCCACTCAAGGA+AGG 0.582843 1.4:+71968720 MS.gene21415:CDS
CTTGCAACAGCTTTGTACCA+TGG 0.587018 1.4:+71967790 MS.gene21415:CDS
TGATTTGTCTAGCCTAAGAA+GGG 0.587869 1.4:+71968482 MS.gene21415:CDS
ATCTTGCCAGCAGCTGACCT+CGG 0.591787 1.4:-71969737 None:intergenic
TCCATATAAGAAAGTGAGAA+GGG 0.594862 1.4:+71969694 MS.gene21415:CDS
CTCCATATAAGAAAGTGAGA+AGG 0.595321 1.4:+71969693 MS.gene21415:CDS
TACCAATGGCATATGTGGTG+AGG 0.595660 1.4:+71969254 MS.gene21415:CDS
ACTGCAACAATTGATTCAGA+AGG 0.598082 1.4:+71968804 MS.gene21415:CDS
AGTATTGAGAGGGTTGGCTG+TGG 0.599920 1.4:-71967914 None:intergenic
ACTGCTGCATCAACAATAAG+GGG 0.600356 1.4:-71969121 None:intergenic
AGTGACATTTCCTTACTCAA+AGG 0.602475 1.4:-71968522 None:intergenic
AACTCTCCTTCCTTGAGTGG+AGG 0.608300 1.4:-71968726 None:intergenic
GCAGATACCAATGGCATATG+TGG 0.613364 1.4:+71969249 MS.gene21415:CDS
AGTCCATAGACATGAAACAT+TGG 0.615670 1.4:-71968291 None:intergenic
AAGGAGCTAATCAAGCACAA+AGG 0.617428 1.4:+71968894 MS.gene21415:CDS
ATGGTACAAAGCTGTTGCAA+GGG 0.627880 1.4:-71967788 None:intergenic
CCCGCCCTTAGTTCAACCCA+AGG 0.635164 1.4:-71968564 None:intergenic
GGGACTACTGGAGTAAGCAA+AGG 0.641617 1.4:+71968168 MS.gene21415:CDS
AGAGTTGCCACATGTTCATG+TGG 0.646575 1.4:+71968110 MS.gene21415:CDS
GTGCTATCACAGTTCCCACA+AGG 0.647014 1.4:+71967679 MS.gene21415:CDS
ACCAATGAGTGGATAACTGG+TGG 0.649415 1.4:-71968435 None:intergenic
GGGTTGAAGATGAAGAAACT+GGG 0.650548 1.4:+71969227 MS.gene21415:intron
AAAGCTCATCCAGAAGCATG+TGG 0.657133 1.4:+71968645 MS.gene21415:CDS
GAAACATTGGAATGAAGGCG+AGG 0.657962 1.4:-71968278 None:intergenic
AGAAACTGGGCAGATACCAA+TGG 0.659161 1.4:+71969240 MS.gene21415:CDS
TATGGACTAACAGAAAGTTG+TGG 0.659761 1.4:+71968588 MS.gene21415:CDS
CTGCAACAAATTGAATGACA+TGG 0.661163 1.4:-71969303 None:intergenic
TAGCATGTTTCACCAATGAG+TGG 0.661752 1.4:-71968446 None:intergenic
TCTCCATTTGATAACAGGAG+CGG 0.666126 1.4:+71967562 MS.gene21415:CDS
CTTCTCGATAGCGACAAGCA+TGG 0.668123 1.4:-71968385 None:intergenic
GGACTAACAGAAAGTTGTGG+GGG 0.674978 1.4:+71968591 MS.gene21415:CDS
GATTGCTCCACATGAACATG+TGG 0.679529 1.4:-71968117 None:intergenic
GTATCAGCAAGATCTCCTTG+TGG 0.682118 1.4:-71967694 None:intergenic
TGGACTAACAGAAAGTTGTG+GGG 0.694783 1.4:+71968590 MS.gene21415:CDS
GGAGAGTTATGGTTGAAAAG+CGG 0.697534 1.4:+71968741 MS.gene21415:CDS
AAGTGATAGACATTGAAACA+GGG 0.705961 1.4:+71968694 MS.gene21415:CDS
CTTTCCATCAGATGTCCCAG+AGG 0.738092 1.4:-71968043 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTTAACTTAAATAATC+AGG - chr1.4:71969452-71969471 None:intergenic 10.0%
!!! AGTAAATTACTTTAATTTTA+AGG + chr1.4:71968996-71969015 MS.gene21415:intron 10.0%
!!! AGTTTTTATTAATACATATG+TGG + chr1.4:71969045-71969064 MS.gene21415:intron 15.0%
!!! TTTGTATTTTAAACCTAATT+AGG - chr1.4:71969495-71969514 None:intergenic 15.0%
!! AGAGAAATTAAAATGACTAT+AGG - chr1.4:71969629-71969648 None:intergenic 20.0%
!! ATAAATAGTTTCAGTTTCAA+TGG + chr1.4:71968936-71968955 MS.gene21415:intron 20.0%
!! ATGAAAATAAGAGTGAAAAT+AGG - chr1.4:71969168-71969187 None:intergenic 20.0%
!! ATTGAAACTGAAACTATTTA+TGG - chr1.4:71968937-71968956 None:intergenic 20.0%
!!! AAATAAATCTGAGAATTTTC+AGG + chr1.4:71969667-71969686 MS.gene21415:intron 20.0%
! AAAATAAGAGTGAAAATAGG+CGG - chr1.4:71969165-71969184 None:intergenic 25.0%
! ACTAATCATAGTGTAACTTA+CGG + chr1.4:71967742-71967761 MS.gene21415:CDS 25.0%
! AGAATATCTAGGAAATATTG+AGG + chr1.4:71968776-71968795 MS.gene21415:CDS 25.0%
! GCTATTATGAAAGAATATCT+AGG + chr1.4:71968765-71968784 MS.gene21415:CDS 25.0%
!! TTAAGGTATCAAATTGAGTT+TGG + chr1.4:71969013-71969032 MS.gene21415:intron 25.0%
!!! TTATTTTACTGCATGAAATC+AGG - chr1.4:71969351-71969370 None:intergenic 25.0%
AAAGTGATAGACATTGAAAC+AGG + chr1.4:71968693-71968712 MS.gene21415:CDS 30.0%
AAGTGATAGACATTGAAACA+GGG + chr1.4:71968694-71968713 MS.gene21415:CDS 30.0%
AGTTATTGCATCTAGATTGT+TGG - chr1.4:71969546-71969565 None:intergenic 30.0%
ATTCCAATGTTTCATGTCTA+TGG + chr1.4:71968288-71968307 MS.gene21415:CDS 30.0%
ATTGTTGGATGAATGTCATA+TGG - chr1.4:71969531-71969550 None:intergenic 30.0%
CTACATAGTTGAACGAATAA+AGG + chr1.4:71968875-71968894 MS.gene21415:CDS 30.0%
GTATCAAATTGAGTTTGGAA+CGG + chr1.4:71969018-71969037 MS.gene21415:intron 30.0%
TATTCTGACTTCACCTAATT+AGG + chr1.4:71969479-71969498 MS.gene21415:intron 30.0%
TCAGTTGAAGAGTTAATAGA+AGG + chr1.4:71968072-71968091 MS.gene21415:CDS 30.0%
TCCATATAAGAAAGTGAGAA+GGG + chr1.4:71969694-71969713 MS.gene21415:CDS 30.0%
TTTCAAGCTTAACAAGAGAA+TGG - chr1.4:71967612-71967631 None:intergenic 30.0%
! ATTTCTGACTTAGTATTGAG+AGG - chr1.4:71967928-71967947 None:intergenic 30.0%
! TTGTAATCATTGTTGCTTAG+TGG + chr1.4:71969381-71969400 MS.gene21415:intron 30.0%
! TTTCTGACTTAGTATTGAGA+GGG - chr1.4:71967927-71967946 None:intergenic 30.0%
!! ATCACTTTTGCACAAAAAGT+TGG - chr1.4:71968681-71968700 None:intergenic 30.0%
!! TAAATCTGAGAATTTTCAGG+TGG + chr1.4:71969670-71969689 MS.gene21415:intron 30.0%
AACTGCTGCATCAACAATAA+GGG - chr1.4:71969125-71969144 None:intergenic 35.0%
ACCAATTTGTGTAACTCTTC+TGG - chr1.4:71967997-71968016 None:intergenic 35.0%
ACCAGAAGAGTTACACAAAT+TGG + chr1.4:71967993-71968012 MS.gene21415:CDS 35.0%
ACCCTTCTCACTTTCTTATA+TGG - chr1.4:71969698-71969717 None:intergenic 35.0%
AGTCCATAGACATGAAACAT+TGG - chr1.4:71968294-71968313 None:intergenic 35.0%
AGTGACATTTCCTTACTCAA+AGG - chr1.4:71968525-71968544 None:intergenic 35.0%
ATGTGGACAAAAAATGTTGC+AGG + chr1.4:71969206-71969225 MS.gene21415:intron 35.0%
CATGGACTTGAGATTAGAAA+AGG + chr1.4:71967808-71967827 MS.gene21415:CDS 35.0%
CTCCATATAAGAAAGTGAGA+AGG + chr1.4:71969693-71969712 MS.gene21415:CDS 35.0%
CTGCAACAAATTGAATGACA+TGG - chr1.4:71969306-71969325 None:intergenic 35.0%
CTGCTATACTTTACTCTTCA+GGG + chr1.4:71968148-71968167 MS.gene21415:CDS 35.0%
CTTGGTTATATCGATGAGAA+TGG + chr1.4:71968846-71968865 MS.gene21415:CDS 35.0%
CTTTGTTGGGATTTCATGTT+TGG - chr1.4:71967635-71967654 None:intergenic 35.0%
GAATGGTAAATTCCTGTTCT+TGG - chr1.4:71967595-71967614 None:intergenic 35.0%
GACATGAAACATTGGAATGA+AGG - chr1.4:71968286-71968305 None:intergenic 35.0%
GACTGATGTTCTTTGGATTT+GGG + chr1.4:71968310-71968329 MS.gene21415:CDS 35.0%
GTTGAGTACAAGATTGAGTT+TGG - chr1.4:71967856-71967875 None:intergenic 35.0%
TAACTGCTGCATCAACAATA+AGG - chr1.4:71969126-71969145 None:intergenic 35.0%
TGATAACACAGCTAAGCATA+TGG - chr1.4:71967878-71967897 None:intergenic 35.0%
TGTGGACAAAAAATGTTGCA+GGG + chr1.4:71969207-71969226 MS.gene21415:intron 35.0%
TTCACCAGATTCTTCTTCAA+CGG - chr1.4:71967536-71967555 None:intergenic 35.0%
TTTGAGTAAGGAAATGTCAC+TGG + chr1.4:71968524-71968543 MS.gene21415:CDS 35.0%
! AATCATTGTTGCTTAGTGGT+TGG + chr1.4:71969385-71969404 MS.gene21415:intron 35.0%
! ACTGCAACAATTGATTCAGA+AGG + chr1.4:71968804-71968823 MS.gene21415:CDS 35.0%
! AGTTCAGAAAGCTATTTCCT+TGG + chr1.4:71968547-71968566 MS.gene21415:CDS 35.0%
! ATGGACTAACAGAAAGTTGT+GGG + chr1.4:71968589-71968608 MS.gene21415:CDS 35.0%
! CAACAATTGATTCAGAAGGT+TGG + chr1.4:71968808-71968827 MS.gene21415:CDS 35.0%
! CTTTTCTAATCTCAAGTCCA+TGG - chr1.4:71967810-71967829 None:intergenic 35.0%
! GTTCAGAAAGCTATTTCCTT+GGG + chr1.4:71968548-71968567 MS.gene21415:CDS 35.0%
! TATGGACTAACAGAAAGTTG+TGG + chr1.4:71968588-71968607 MS.gene21415:CDS 35.0%
! TGATTTGTCTAGCCTAAGAA+GGG + chr1.4:71968482-71968501 MS.gene21415:CDS 35.0%
!! ATTTCCTTGGGTTGAACTAA+GGG + chr1.4:71968560-71968579 MS.gene21415:CDS 35.0%
!! CATGTCATTCAATTTGTTGC+AGG + chr1.4:71969304-71969323 MS.gene21415:CDS 35.0%
!! GTTTTATTGACAGTATACCG+AGG + chr1.4:71969720-71969739 MS.gene21415:CDS 35.0%
!! TATTTCCTTGGGTTGAACTA+AGG + chr1.4:71968559-71968578 MS.gene21415:CDS 35.0%
AAGGAGCTAATCAAGCACAA+AGG + chr1.4:71968894-71968913 MS.gene21415:CDS 40.0%
ACTGCTGCATCAACAATAAG+GGG - chr1.4:71969124-71969143 None:intergenic 40.0%
AGAATTGTAGGAACTCCAGT+TGG - chr1.4:71968021-71968040 None:intergenic 40.0%
AGGAGCTAATCAAGCACAAA+GGG + chr1.4:71968895-71968914 MS.gene21415:CDS 40.0%
AGGGTTGAAGATGAAGAAAC+TGG + chr1.4:71969226-71969245 MS.gene21415:intron 40.0%
ATAAGAGTGAAAATAGGCGG+AGG - chr1.4:71969162-71969181 None:intergenic 40.0%
ATGGTACAAAGCTGTTGCAA+GGG - chr1.4:71967791-71967810 None:intergenic 40.0%
CTATAGGAAAACGAGAAAGC+AGG - chr1.4:71969613-71969632 None:intergenic 40.0%
CTCTCTGTGACTTTCTTATG+TGG + chr1.4:71969189-71969208 MS.gene21415:intron 40.0%
GAATTGTAGGAACTCCAGTT+GGG - chr1.4:71968020-71968039 None:intergenic 40.0%
GCTGCTATACTTTACTCTTC+AGG + chr1.4:71968147-71968166 MS.gene21415:CDS 40.0%
GGACTGATGTTCTTTGGATT+TGG + chr1.4:71968309-71968328 MS.gene21415:CDS 40.0%
GGAGAGTTATGGTTGAAAAG+CGG + chr1.4:71968741-71968760 MS.gene21415:CDS 40.0%
GGGTTGAAGATGAAGAAACT+GGG + chr1.4:71969227-71969246 MS.gene21415:intron 40.0%
GTGAAACATGCTAGCAAAGA+TGG + chr1.4:71968456-71968475 MS.gene21415:CDS 40.0%
TAAGAGTGAAAATAGGCGGA+GGG - chr1.4:71969161-71969180 None:intergenic 40.0%
TAGCATGTTTCACCAATGAG+TGG - chr1.4:71968449-71968468 None:intergenic 40.0%
TATCAGCAAGATCTCCTTGT+GGG - chr1.4:71967696-71967715 None:intergenic 40.0%
TATCCGCTCCTGTTATCAAA+TGG - chr1.4:71967568-71967587 None:intergenic 40.0%
TCATCCGTTGAAGAAGAATC+TGG + chr1.4:71967529-71967548 MS.gene21415:CDS 40.0%
TGCTTAGCTGTGTTATCAGT+TGG + chr1.4:71967880-71967899 MS.gene21415:CDS 40.0%
TGGACTAACAGAAAGTTGTG+GGG + chr1.4:71968590-71968609 MS.gene21415:CDS 40.0%
TTACACAAATTGGTCCCAAC+TGG + chr1.4:71968003-71968022 MS.gene21415:CDS 40.0%
TTCACCAATGAGTGGATAAC+TGG - chr1.4:71968441-71968460 None:intergenic 40.0%
TTCCTACAATTCTCACCTCT+GGG + chr1.4:71968028-71968047 MS.gene21415:CDS 40.0%
! ATATGTGGATTGCTTGTGTC+AGG + chr1.4:71969060-71969079 MS.gene21415:intron 40.0%
! CTCTGTCTCCATTTGATAAC+AGG + chr1.4:71967557-71967576 MS.gene21415:CDS 40.0%
! GTCTATGGACTGATGTTCTT+TGG + chr1.4:71968303-71968322 MS.gene21415:CDS 40.0%
! GTGATTTGTCTAGCCTAAGA+AGG + chr1.4:71968481-71968500 MS.gene21415:CDS 40.0%
! TCTCCATTTGATAACAGGAG+CGG + chr1.4:71967562-71967581 MS.gene21415:CDS 40.0%
! TGACTTAGTATTGAGAGGGT+TGG - chr1.4:71967923-71967942 None:intergenic 40.0%
! TTGATTCAGAAGGTTGGTTG+AGG + chr1.4:71968814-71968833 MS.gene21415:CDS 40.0%
!! AATCAAGCACAAAGGGTATC+AGG + chr1.4:71968902-71968921 MS.gene21415:CDS 40.0%
!! TGTTGAGATCTGGCTTTGTT+GGG - chr1.4:71967648-71967667 None:intergenic 40.0%
AAGAGTGAAAATAGGCGGAG+GGG - chr1.4:71969160-71969179 None:intergenic 45.0%
ACCAATGAGTGGATAACTGG+TGG - chr1.4:71968438-71968457 None:intergenic 45.0%
ACCAATGGCATATGTGGTGA+GGG + chr1.4:71969255-71969274 MS.gene21415:CDS 45.0%
AGAAACTGGGCAGATACCAA+TGG + chr1.4:71969240-71969259 MS.gene21415:CDS 45.0%
AGAGTTGCCACATGTTCATG+TGG + chr1.4:71968110-71968129 MS.gene21415:CDS 45.0%
ATCAGCTTTCCACATGCTTC+TGG - chr1.4:71968657-71968676 None:intergenic 45.0%
ATGAAATCAGGAGCCAAGAG+TGG - chr1.4:71969339-71969358 None:intergenic 45.0%
CACTCAAGGAAGGAGAGTTA+TGG + chr1.4:71968730-71968749 MS.gene21415:CDS 45.0%
CATAACTCTCCTTCCTTGAG+TGG - chr1.4:71968732-71968751 None:intergenic 45.0%
CATGGTACAAAGCTGTTGCA+AGG - chr1.4:71967792-71967811 None:intergenic 45.0%
CCAGCAGAGCAGTTTCATTA+TGG - chr1.4:71968223-71968242 None:intergenic 45.0%
CCATAATGAAACTGCTCTGC+TGG + chr1.4:71968220-71968239 MS.gene21415:CDS 45.0%
CTTTACTCTTCAGGGACTAC+TGG + chr1.4:71968156-71968175 MS.gene21415:CDS 45.0%
GAAACATTGGAATGAAGGCG+AGG - chr1.4:71968281-71968300 None:intergenic 45.0%
GATTGCTCCACATGAACATG+TGG - chr1.4:71968120-71968139 None:intergenic 45.0%
GCAGATACCAATGGCATATG+TGG + chr1.4:71969249-71969268 MS.gene21415:CDS 45.0%
GCGAAGCAAGTACATGATTC+AGG + chr1.4:71967949-71967968 MS.gene21415:CDS 45.0%
GGACTAACAGAAAGTTGTGG+GGG + chr1.4:71968591-71968610 MS.gene21415:CDS 45.0%
GGAGCTAAGAGAAGCAGAAA+TGG - chr1.4:71969592-71969611 None:intergenic 45.0%
GTATCAGCAAGATCTCCTTG+TGG - chr1.4:71967697-71967716 None:intergenic 45.0%
GTTCCTACAATTCTCACCTC+TGG + chr1.4:71968027-71968046 MS.gene21415:CDS 45.0%
TACCAATGGCATATGTGGTG+AGG + chr1.4:71969254-71969273 MS.gene21415:CDS 45.0%
TTGTCTAGCCTAAGAAGGGT+TGG + chr1.4:71968486-71968505 MS.gene21415:CDS 45.0%
! AAAGCTCATCCAGAAGCATG+TGG + chr1.4:71968645-71968664 MS.gene21415:CDS 45.0%
! AGAGTTGCAGTGTTGAGATC+TGG - chr1.4:71967658-71967677 None:intergenic 45.0%
! CTTGCAACAGCTTTGTACCA+TGG + chr1.4:71967790-71967809 MS.gene21415:CDS 45.0%
!! CATTCAATTTGTTGCAGGCC+AGG + chr1.4:71969309-71969328 MS.gene21415:CDS 45.0%
!! GGATTTGGGTTGTTGTGTGT+TGG + chr1.4:71968324-71968343 MS.gene21415:CDS 45.0%
!! GTGTTGAGATCTGGCTTTGT+TGG - chr1.4:71967649-71967668 None:intergenic 45.0%
AACTCTCCTTCCTTGAGTGG+AGG - chr1.4:71968729-71968748 None:intergenic 50.0%
AGCACAGATTCCAGTTCTGC+AGG - chr1.4:71969091-71969110 None:intergenic 50.0%
AGCGGATACGATCCAAGAAC+AGG + chr1.4:71967580-71967599 MS.gene21415:CDS 50.0%
CTTCTCGATAGCGACAAGCA+TGG - chr1.4:71968388-71968407 None:intergenic 50.0%
CTTTCCATCAGATGTCCCAG+AGG - chr1.4:71968046-71968065 None:intergenic 50.0%
GCCACCAGTTATCCACTCAT+TGG + chr1.4:71968434-71968453 MS.gene21415:CDS 50.0%
GGGACTACTGGAGTAAGCAA+AGG + chr1.4:71968168-71968187 MS.gene21415:CDS 50.0%
GTCCCAGAGGTGAGAATTGT+AGG - chr1.4:71968033-71968052 None:intergenic 50.0%
GTGCTATCACAGTTCCCACA+AGG + chr1.4:71967679-71967698 MS.gene21415:CDS 50.0%
TGGTTGAGGACAGGTGATCT+TGG + chr1.4:71968828-71968847 MS.gene21415:CDS 50.0%
! AGGTGCTGCACCTTTGAGTA+AGG + chr1.4:71968512-71968531 MS.gene21415:CDS 50.0%
! TCAGAAGGTTGGTTGAGGAC+AGG + chr1.4:71968819-71968838 MS.gene21415:CDS 50.0%
! TGTGGGGGAGCTACGTTTTT+CGG + chr1.4:71968606-71968625 MS.gene21415:CDS 50.0%
!! AGTATTGAGAGGGTTGGCTG+TGG - chr1.4:71967917-71967936 None:intergenic 50.0%
!! CAATTTGTTGCAGGCCAGGT+AGG + chr1.4:71969313-71969332 MS.gene21415:intron 50.0%
!! GTTGAACTAAGGGCGGGTTA+TGG + chr1.4:71968570-71968589 MS.gene21415:CDS 50.0%
!! TCCTTGGGTTGAACTAAGGG+CGG + chr1.4:71968563-71968582 MS.gene21415:CDS 50.0%
AATAGGCGGAGGGGATCGTA+CGG - chr1.4:71969151-71969170 None:intergenic 55.0%
AGTATACCGAGGTCAGCTGC+TGG + chr1.4:71969731-71969750 MS.gene21415:CDS 55.0%
ATCTTGCCAGCAGCTGACCT+CGG - chr1.4:71969740-71969759 None:intergenic 55.0%
CACCTGAGCCAACCCTTCTT+AGG - chr1.4:71968497-71968516 None:intergenic 55.0%
CCTTCCTTGAGTGGAGGCAA+AGG - chr1.4:71968723-71968742 None:intergenic 55.0%
CCTTTGCCTCCACTCAAGGA+AGG + chr1.4:71968720-71968739 MS.gene21415:CDS 55.0%
GAAGCCTTTGCCTCCACTCA+AGG + chr1.4:71968716-71968735 MS.gene21415:CDS 55.0%
GCCCTCACCACATATGCCAT+TGG - chr1.4:71969259-71969278 None:intergenic 55.0%
TCAGGTAGCTCCTGCAGAAC+TGG + chr1.4:71969078-71969097 MS.gene21415:intron 55.0%
TGGATAACTGGTGGCACTGC+TGG - chr1.4:71968429-71968448 None:intergenic 55.0%
! GTGGGGGAGCTACGTTTTTC+GGG + chr1.4:71968607-71968626 MS.gene21415:CDS 55.0%
!! AGCCTAAGAAGGGTTGGCTC+AGG + chr1.4:71968492-71968511 MS.gene21415:CDS 55.0%
!! CCTTGGGTTGAACTAAGGGC+GGG + chr1.4:71968564-71968583 MS.gene21415:CDS 55.0%
!! CTCACCTCTGGGACATCTGA+TGG + chr1.4:71968039-71968058 MS.gene21415:CDS 55.0%
!!! GGGAGCTACGTTTTTCGGGT+CGG + chr1.4:71968611-71968630 MS.gene21415:CDS 55.0%
CCCGCCCTTAGTTCAACCCA+AGG - chr1.4:71968567-71968586 None:intergenic 60.0%
GCATATGTGGTGAGGGCAGC+TGG + chr1.4:71969262-71969281 MS.gene21415:CDS 60.0%
CAGGCCAGGTAGGCCACTCT+TGG + chr1.4:71969323-71969342 MS.gene21415:intron 65.0%
GGAGCCAAGAGTGGCCTACC+TGG - chr1.4:71969330-71969349 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 71967508 71969814 71967508 ID=MS.gene21415
chr1.4 mRNA 71967508 71969814 71967508 ID=MS.gene21415.t1;Parent=MS.gene21415
chr1.4 exon 71967508 71968923 71967508 ID=MS.gene21415.t1.exon1;Parent=MS.gene21415.t1
chr1.4 CDS 71967508 71968923 71967508 ID=cds.MS.gene21415.t1;Parent=MS.gene21415.t1
chr1.4 exon 71969082 71969149 71969082 ID=MS.gene21415.t1.exon2;Parent=MS.gene21415.t1
chr1.4 CDS 71969082 71969149 71969082 ID=cds.MS.gene21415.t1;Parent=MS.gene21415.t1
chr1.4 exon 71969228 71969330 71969228 ID=MS.gene21415.t1.exon3;Parent=MS.gene21415.t1
chr1.4 CDS 71969228 71969330 71969228 ID=cds.MS.gene21415.t1;Parent=MS.gene21415.t1
chr1.4 exon 71969689 71969814 71969689 ID=MS.gene21415.t1.exon4;Parent=MS.gene21415.t1
chr1.4 CDS 71969689 71969814 71969689 ID=cds.MS.gene21415.t1;Parent=MS.gene21415.t1
Gene Sequence

>MS.gene21415

ATGTCAGGAGAGGAGATTTGGTCATCCGTTGAAGAAGAATCTGGTGAATCTCTGTCTCCATTTGATAACAGGAGCGGATACGATCCAAGAACAGGAATTTACCATTCTCTTGTTAAGCTTGAAACCAAACATGAAATCCCAACAAAGCCAGATCTCAACACTGCAACTCTTGTGCTATCACAGTTCCCACAAGGAGATCTTGCTGATACAAGAATTGCATTCATTGACTTAAATACTAATCATAGTGTAACTTACGGTGAGATTCGTCGATCAGTTTACTCCCTTGCAACAGCTTTGTACCATGGACTTGAGATTAGAAAAGGCGACGTCGTGTTTTTATTGTCTCCAAACTCAATCTTGTACTCAACCATATGCTTAGCTGTGTTATCAGTTGGTGCAATTCTTACCACAGCCAACCCTCTCAATACTAAGTCAGAAATCGCGAAGCAAGTACATGATTCAGGTGCTAAACTAGCTATCTCAGCACCAGAAGAGTTACACAAATTGGTCCCAACTGGAGTTCCTACAATTCTCACCTCTGGGACATCTGATGGAAAGTTTTTATCAGTTGAAGAGTTAATAGAAGGCTGCTATGATTCACAAGAGTTGCCACATGTTCATGTGGAGCAATCAGACACTGCTGCTATACTTTACTCTTCAGGGACTACTGGAGTAAGCAAAGGTGTTGTTTTGACACATGCAAATCTCATAACCATAATGAAACTGCTCTGCTGGTCTGCTGATGTATCTACAGCACAAGACGATGTTTTCCTCGCCTTCATTCCAATGTTTCATGTCTATGGACTGATGTTCTTTGGATTTGGGTTGTTGTGTGTTGGTGTTACGACAGTTTTGATGCAAAAATATGATTTTCAAGCCATGCTTGTCGCTATCGAGAAGCACAAAGTTAATAACATACCAGCAGTGCCACCAGTTATCCACTCATTGGTGAAACATGCTAGCAAAGATGGCTGTGATTTGTCTAGCCTAAGAAGGGTTGGCTCAGGTGCTGCACCTTTGAGTAAGGAAATGTCACTGGAGTTCAGAAAGCTATTTCCTTGGGTTGAACTAAGGGCGGGTTATGGACTAACAGAAAGTTGTGGGGGAGCTACGTTTTTCGGGTCGGATAAAGATGCTAAAGCTCATCCAGAAGCATGTGGAAAGCTGATTCCAACTTTTTGTGCAAAAGTGATAGACATTGAAACAGGGAAGCCTTTGCCTCCACTCAAGGAAGGAGAGTTATGGTTGAAAAGCGGTGCTATTATGAAAGAATATCTAGGAAATATTGAGGCAACAACTGCAACAATTGATTCAGAAGGTTGGTTGAGGACAGGTGATCTTGGTTATATCGATGAGAATGGAATTGTCTACATAGTTGAACGAATAAAGGAGCTAATCAAGCACAAAGGGTATCAGGTATTCAAAGCCATAAATAGTTTCAGTTTCAATGGTGAAGTTTTGTTTCACTTAAGTTAAAATTAGAATATCAGTAAATTACTTTAATTTTAAGGTATCAAATTGAGTTTGGAACGGTTTTAGTTTTTATTAATACATATGTGGATTGCTTGTGTCAGGTAGCTCCTGCAGAACTGGAATCTGTGCTGCTAAGTCATCCCCTTATTGTTGATGCAGCAGTTATTCCGTACGATCCCCTCCGCCTATTTTCACTCTTATTTTCATTCTCTCTGTGACTTTCTTATGTGGACAAAAAATGTTGCAGGGTTGAAGATGAAGAAACTGGGCAGATACCAATGGCATATGTGGTGAGGGCAGCTGGTTCTCAACTATCAGAAGACCATGTCATTCAATTTGTTGCAGGCCAGGTAGGCCACTCTTGGCTCCTGATTTCATGCAGTAAAATAATCTGCAGTCATTGTAATCATTGTTGCTTAGTGGTTGGATGAAGATCAAACAATTCATATTTTAAGTTTGTATTTTAAACCTGATTATTTAAGTTAAATTTTAAATTCATATTCTGACTTCACCTAATTAGGTTTAAAATACAAAACTGAAATAAAAACCATATGACATTCATCCAACAATCTAGATGCAATAACTGCAGATATTTTACTTCATCAAGTCCATTTCTGCTTCTCTTAGCTCCTGCTTTCTCGTTTTCCTATAGTCATTTTAATTTCTCTCTCTTTATTTTTAAATTAAAATAAATCTGAGAATTTTCAGGTGGCTCCATATAAGAAAGTGAGAAGGGTGAGTTTTATTGACAGTATACCGAGGTCAGCTGCTGGCAAGATATTGCGAAAAGATCTTGTTTTGCAAAGCAAATACCAGCTTGTTTCTAAGTTGTGA
Protein sequence

>MS.gene21415.t1

MSGEEIWSSVEEESGESLSPFDNRSGYDPRTGIYHSLVKLETKHEIPTKPDLNTATLVLSQFPQGDLADTRIAFIDLNTNHSVTYGEIRRSVYSLATALYHGLEIRKGDVVFLLSPNSILYSTICLAVLSVGAILTTANPLNTKSEIAKQVHDSGAKLAISAPEELHKLVPTGVPTILTSGTSDGKFLSVEELIEGCYDSQELPHVHVEQSDTAAILYSSGTTGVSKGVVLTHANLITIMKLLCWSADVSTAQDDVFLAFIPMFHVYGLMFFGFGLLCVGVTTVLMQKYDFQAMLVAIEKHKVNNIPAVPPVIHSLVKHASKDGCDLSSLRRVGSGAAPLSKEMSLEFRKLFPWVELRAGYGLTESCGGATFFGSDKDAKAHPEACGKLIPTFCAKVIDIETGKPLPPLKEGELWLKSGAIMKEYLGNIEATTATIDSEGWLRTGDLGYIDENGIVYIVERIKELIKHKGYQVAPAELESVLLSHPLIVDAAVIPVEDEETGQIPMAYVVRAAGSQLSEDHVIQFVAGQVAPYKKVRRVSFIDSIPRSAAGKILRKDLVLQSKYQLVSKL