Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21535.t1 | XP_003611369.1 | 96.7 | 548 | 16 | 2 | 1 | 546 | 1 | 548 | 8.80E-297 | 1028.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21535.t1 | O23522 | 55.4 | 531 | 197 | 10 | 25 | 546 | 18 | 517 | 5.3e-157 | 555.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21535.t1 | G7JXH0 | 96.7 | 548 | 16 | 2 | 1 | 546 | 1 | 548 | 6.3e-297 | 1028.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050238 | MS.gene21535 | 0.820031 | 8.50E-53 | -1.69E-46 |
| MS.gene051823 | MS.gene21535 | 0.808001 | 3.84E-50 | -1.69E-46 |
| MS.gene052016 | MS.gene21535 | 0.835789 | 1.37E-56 | -1.69E-46 |
| MS.gene053620 | MS.gene21535 | 0.804869 | 1.76E-49 | -1.69E-46 |
| MS.gene054178 | MS.gene21535 | 0.807813 | 4.21E-50 | -1.69E-46 |
| MS.gene054892 | MS.gene21535 | 0.804089 | 2.55E-49 | -1.69E-46 |
| MS.gene060522 | MS.gene21535 | 0.80766 | 4.54E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21535.t1 | MTR_5g013230 | 97.628 | 548 | 11 | 2 | 1 | 546 | 1 | 548 | 0.0 | 1110 |
| MS.gene21535.t1 | MTR_4g099510 | 65.766 | 555 | 137 | 9 | 6 | 544 | 4 | 521 | 0.0 | 715 |
| MS.gene21535.t1 | MTR_7g081620 | 47.165 | 388 | 177 | 8 | 129 | 492 | 65 | 448 | 1.57e-120 | 362 |
| MS.gene21535.t1 | MTR_7g081645 | 45.553 | 371 | 167 | 9 | 133 | 492 | 75 | 421 | 8.94e-110 | 334 |
| MS.gene21535.t1 | MTR_4g077070 | 38.281 | 384 | 201 | 7 | 128 | 493 | 95 | 460 | 3.97e-84 | 271 |
| MS.gene21535.t1 | MTR_7g096120 | 39.737 | 380 | 194 | 11 | 133 | 493 | 74 | 437 | 1.12e-81 | 264 |
| MS.gene21535.t1 | MTR_7g096140 | 39.211 | 380 | 196 | 11 | 133 | 493 | 74 | 437 | 5.42e-81 | 261 |
| MS.gene21535.t1 | MTR_2g015690 | 35.156 | 384 | 192 | 9 | 133 | 493 | 20 | 369 | 1.28e-63 | 214 |
| MS.gene21535.t1 | MTR_7g006400 | 32.683 | 410 | 215 | 13 | 109 | 500 | 80 | 446 | 4.17e-57 | 199 |
| MS.gene21535.t1 | MTR_4g087810 | 33.075 | 387 | 195 | 11 | 133 | 495 | 13 | 359 | 6.13e-52 | 182 |
| MS.gene21535.t1 | MTR_4g087830 | 33.333 | 387 | 194 | 12 | 133 | 495 | 4 | 350 | 2.82e-51 | 181 |
| MS.gene21535.t1 | MTR_4g080100 | 29.353 | 402 | 214 | 9 | 129 | 493 | 24 | 392 | 1.01e-48 | 175 |
| MS.gene21535.t1 | MTR_1995s0010 | 40.928 | 237 | 114 | 7 | 251 | 475 | 1 | 223 | 5.20e-45 | 158 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21535.t1 | AT4G16820 | 55.367 | 531 | 197 | 10 | 25 | 546 | 18 | 517 | 0.0 | 562 |
| MS.gene21535.t1 | AT2G44810 | 50.136 | 369 | 170 | 7 | 133 | 494 | 86 | 447 | 5.25e-123 | 369 |
| MS.gene21535.t1 | AT2G44810 | 50.413 | 363 | 166 | 7 | 139 | 494 | 2 | 357 | 1.95e-120 | 359 |
| MS.gene21535.t1 | AT1G30370 | 38.015 | 413 | 219 | 10 | 124 | 517 | 105 | 499 | 6.99e-86 | 276 |
| MS.gene21535.t1 | AT1G51440 | 41.463 | 369 | 172 | 10 | 137 | 481 | 91 | 439 | 3.66e-83 | 268 |
| MS.gene21535.t1 | AT1G06800 | 39.633 | 381 | 187 | 10 | 137 | 493 | 89 | 450 | 5.88e-79 | 257 |
| MS.gene21535.t1 | AT2G30550 | 39.227 | 362 | 186 | 7 | 137 | 479 | 105 | 451 | 9.75e-79 | 255 |
| MS.gene21535.t1 | AT1G06800 | 40.385 | 364 | 174 | 10 | 137 | 476 | 89 | 433 | 1.11e-78 | 254 |
| MS.gene21535.t1 | AT2G30550 | 38.830 | 376 | 196 | 8 | 137 | 493 | 105 | 465 | 6.99e-78 | 255 |
| MS.gene21535.t1 | AT2G31690 | 35.989 | 364 | 182 | 11 | 133 | 475 | 88 | 421 | 2.66e-65 | 220 |
| MS.gene21535.t1 | AT1G05800 | 35.833 | 360 | 185 | 9 | 133 | 475 | 80 | 410 | 1.00e-63 | 216 |
| MS.gene21535.t1 | AT4G18550 | 33.012 | 415 | 210 | 11 | 133 | 515 | 19 | 397 | 1.85e-60 | 206 |
| MS.gene21535.t1 | AT4G18550 | 33.012 | 415 | 210 | 11 | 133 | 515 | 36 | 414 | 2.72e-60 | 206 |
| MS.gene21535.t1 | AT1G06250 | 31.959 | 388 | 200 | 10 | 133 | 493 | 5 | 355 | 6.52e-54 | 188 |
| MS.gene21535.t1 | AT2G42690 | 32.468 | 385 | 204 | 8 | 137 | 493 | 8 | 364 | 3.55e-51 | 181 |
| MS.gene21535.t1 | AT4G18550 | 36.806 | 288 | 151 | 8 | 133 | 397 | 36 | 315 | 4.56e-47 | 167 |
| MS.gene21535.t1 | AT1G06250 | 30.797 | 276 | 140 | 7 | 234 | 493 | 1 | 241 | 5.10e-32 | 125 |
Find 139 sgRNAs with CRISPR-Local
Find 154 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCAATTGGATTACCTAAA+TGG | 0.127660 | 5.3:+6206575 | MS.gene21535:CDS |
| CCTCGGGTAAATCGACTAAT+TGG | 0.206931 | 5.3:-6206768 | None:intergenic |
| GAAGACGCTGTAAGCGTTTC+AGG | 0.238955 | 5.3:-6206288 | None:intergenic |
| GTTACTTTATAAGATCTTTC+CGG | 0.250527 | 5.3:-6206532 | None:intergenic |
| TAACCTAGATGAGTTGTTAT+TGG | 0.251262 | 5.3:-6206077 | None:intergenic |
| CTCTTAGCATTAGCTCTAAA+TGG | 0.258178 | 5.3:-6207396 | None:intergenic |
| CGCCTTTGTAAAGTTCAATA+AGG | 0.276950 | 5.3:-6206921 | None:intergenic |
| AAAAGATGAAGGTTGTTTAA+TGG | 0.278707 | 5.3:-6206041 | None:intergenic |
| TTTCTCCCGATGATATTTCT+TGG | 0.282496 | 5.3:-6206334 | None:intergenic |
| AGAGGCTTATTTACATTTAG+TGG | 0.289753 | 5.3:+6207350 | MS.gene21535:CDS |
| ATGATGATTCCAAAGGCTTT+TGG | 0.306541 | 5.3:-6206132 | None:intergenic |
| GCCACCTCATCAACCCATTT+AGG | 0.326253 | 5.3:-6206589 | None:intergenic |
| GTTGTATTGGTTGTTGAAAC+TGG | 0.332759 | 5.3:-6206162 | None:intergenic |
| ACTCAACTTTGGGCTTTGAT+TGG | 0.339758 | 5.3:-6206819 | None:intergenic |
| ACAGCCACGGGTGGCACGTT+TGG | 0.344135 | 5.3:-6207027 | None:intergenic |
| TAATTTCCTCGGCCACCAAT+AGG | 0.366166 | 5.3:-6206993 | None:intergenic |
| CGGCGAGAGGTTGTTAGATA+TGG | 0.368122 | 5.3:+6206421 | MS.gene21535:CDS |
| CATAAATCCACACTCAACTT+TGG | 0.371336 | 5.3:-6206830 | None:intergenic |
| CTGTAAGCGTTTCAGGTGGC+GGG | 0.378709 | 5.3:-6206281 | None:intergenic |
| TCTTCAGCTGCTTTAATCTC+TGG | 0.381476 | 5.3:-6206250 | None:intergenic |
| TGGCGCGACACTAGCCCTAT+TGG | 0.385796 | 5.3:+6206978 | MS.gene21535:CDS |
| AACTTCCACTTGTCTTGAAT+GGG | 0.386150 | 5.3:+6206729 | MS.gene21535:CDS |
| GTTGGAAGAGAACACTCCTT+TGG | 0.394244 | 5.3:+6207236 | MS.gene21535:CDS |
| CGTGTTGGTAACAGAGCCTT+TGG | 0.394565 | 5.3:+6207069 | MS.gene21535:CDS |
| TAAAAGAGAGATTGCAAGAA+TGG | 0.395214 | 5.3:+6206675 | MS.gene21535:CDS |
| ATAAATCCACACTCAACTTT+GGG | 0.396883 | 5.3:-6206829 | None:intergenic |
| TCTTGATAGCCAAAAGCCTT+TGG | 0.405866 | 5.3:+6206123 | MS.gene21535:CDS |
| GGATGACCCAGCGTTCTAGC+TGG | 0.407435 | 5.3:+6206626 | MS.gene21535:CDS |
| ATCAATTGGATTACCTAAAT+GGG | 0.408997 | 5.3:+6206576 | MS.gene21535:CDS |
| GGTTGTTAGATATGGTGATT+TGG | 0.409569 | 5.3:+6206429 | MS.gene21535:CDS |
| GAACTTCCACTTGTCTTGAA+TGG | 0.412888 | 5.3:+6206728 | MS.gene21535:CDS |
| GACTTAACCTAGCAAAACTA+TGG | 0.417487 | 5.3:+6206227 | MS.gene21535:CDS |
| CTGTATGCCACATCATCAAT+TGG | 0.418121 | 5.3:+6206562 | MS.gene21535:CDS |
| TCATCAAGAGGTGTCGTTGC+CGG | 0.419070 | 5.3:+6206513 | MS.gene21535:CDS |
| AATTTCCTCGGCCACCAATA+GGG | 0.421437 | 5.3:-6206992 | None:intergenic |
| ATGAGCTTGTACAAGACAAA+AGG | 0.423174 | 5.3:+6206850 | MS.gene21535:CDS |
| TTGGAAGAGAACACTCCTTT+GGG | 0.425192 | 5.3:+6207237 | MS.gene21535:CDS |
| GAATTAAGCATAACCGTTAC+CGG | 0.425326 | 5.3:+6206946 | MS.gene21535:CDS |
| AAGAATTCCAAGGTTGTTAG+TGG | 0.426321 | 5.3:+6207201 | MS.gene21535:CDS |
| ACATTTAGTGGATGGATACA+TGG | 0.441165 | 5.3:+6207362 | MS.gene21535:CDS |
| AAACCAATAACAACTCATCT+AGG | 0.443716 | 5.3:+6206074 | MS.gene21535:CDS |
| AATATCATGGCAGCAACGAC+TGG | 0.446528 | 5.3:+6206365 | MS.gene21535:CDS |
| GATGAGGTGGCACCAGATCT+TGG | 0.447657 | 5.3:+6206601 | MS.gene21535:CDS |
| CCCGCACGTGCTAATTTCCT+CGG | 0.448005 | 5.3:-6207004 | None:intergenic |
| TAGACAGGGATCCATGTCTA+TGG | 0.450095 | 5.3:+6207536 | MS.gene21535:CDS |
| GTTGTTTAATGGAAGAAGAA+TGG | 0.450283 | 5.3:-6206030 | None:intergenic |
| CACTTCTTCAACAACGGACT+CGG | 0.454486 | 5.3:-6206893 | None:intergenic |
| GTGAAGGTTGTGAGCAGGTA+AGG | 0.457213 | 5.3:-6205972 | None:intergenic |
| GTAAGCGTTTCAGGTGGCGG+GGG | 0.458362 | 5.3:-6206279 | None:intergenic |
| CTTGTCTAATCTTAGTAGAC+AGG | 0.459042 | 5.3:+6207521 | MS.gene21535:CDS |
| CTAGTGTCGCGCCAAGGCTA+TGG | 0.467757 | 5.3:-6206969 | None:intergenic |
| GCTGTAAGCGTTTCAGGTGG+CGG | 0.475077 | 5.3:-6206282 | None:intergenic |
| GTCTGAGTCTGAGCGTCCTC+GGG | 0.477426 | 5.3:-6206784 | None:intergenic |
| TCGCCGGAGGTTGTCATCCA+AGG | 0.480124 | 5.3:-6206404 | None:intergenic |
| GAAGGGGATGTTAGATCCCT+TGG | 0.480349 | 5.3:+6206387 | MS.gene21535:CDS |
| TGGGTCATCCAACCAAGATC+TGG | 0.480411 | 5.3:-6206613 | None:intergenic |
| TGAAGAGAAGCAAAAGATGA+AGG | 0.480894 | 5.3:-6206052 | None:intergenic |
| GCTTCAGTTGTTGACATGGC+AGG | 0.485069 | 5.3:-6206484 | None:intergenic |
| TGGAGAATACTCCGCCGTCT+TGG | 0.486287 | 5.3:-6206314 | None:intergenic |
| TATTGGTTGTTGAAACTGGT+TGG | 0.487668 | 5.3:-6206158 | None:intergenic |
| AGTCTGAGTCTGAGCGTCCT+CGG | 0.490474 | 5.3:-6206785 | None:intergenic |
| AAAAGAGAGATTGCAAGAAT+GGG | 0.490721 | 5.3:+6206676 | MS.gene21535:CDS |
| ACACACAAGACGTGATTACA+AGG | 0.492272 | 5.3:+6207136 | MS.gene21535:CDS |
| CAACGACACCTCTTGATGAT+GGG | 0.496077 | 5.3:-6206509 | None:intergenic |
| CTCGGGTAAATCGACTAATT+GGG | 0.499666 | 5.3:-6206767 | None:intergenic |
| AACAACTCCACTAACAACCT+TGG | 0.499945 | 5.3:-6207208 | None:intergenic |
| GCAACGACACCTCTTGATGA+TGG | 0.502309 | 5.3:-6206510 | None:intergenic |
| TGAGCTTGTACAAGACAAAA+GGG | 0.503559 | 5.3:+6206851 | MS.gene21535:CDS |
| CCTTTGGGATATTCACATGT+TGG | 0.504000 | 5.3:+6207252 | MS.gene21535:CDS |
| GAAATATCATCGGGAGAAAG+TGG | 0.505201 | 5.3:+6206338 | MS.gene21535:CDS |
| CTTCAACAACGGACTCGGAT+AGG | 0.508893 | 5.3:-6206888 | None:intergenic |
| TCAGCATCGGGCTTCAAGTA+AGG | 0.510052 | 5.3:-6207306 | None:intergenic |
| CAACAAGCTATGTCAGCATC+GGG | 0.515189 | 5.3:-6207318 | None:intergenic |
| GCTTATTTACATTTAGTGGA+TGG | 0.516513 | 5.3:+6207354 | MS.gene21535:CDS |
| GATGACCCAGCGTTCTAGCT+GGG | 0.518537 | 5.3:+6206627 | MS.gene21535:CDS |
| ACCCAGCGTTCTAGCTGGGT+TGG | 0.519887 | 5.3:+6206631 | MS.gene21535:CDS |
| GAAGAATGGCATCTGAAACT+AGG | 0.523536 | 5.3:-6206016 | None:intergenic |
| TTGTATACTAGTAAGGCTAA+AGG | 0.526986 | 5.3:+6207465 | MS.gene21535:CDS |
| ATCCCTTGGATGACAACCTC+CGG | 0.528647 | 5.3:+6206401 | MS.gene21535:CDS |
| ATCCAACCCAGCTAGAACGC+TGG | 0.529696 | 5.3:-6206633 | None:intergenic |
| GACGTGATTACAAGGGTGCC+AGG | 0.535150 | 5.3:+6207144 | MS.gene21535:CDS |
| CGCGACACTAGCCCTATTGG+TGG | 0.536052 | 5.3:+6206981 | MS.gene21535:CDS |
| TAGTGGAGTTGTTGATATGT+TGG | 0.537325 | 5.3:+6207218 | MS.gene21535:CDS |
| TGTAAGCGTTTCAGGTGGCG+GGG | 0.538613 | 5.3:-6206280 | None:intergenic |
| ACCGTTACCGGCCATAGCCT+TGG | 0.539736 | 5.3:+6206958 | MS.gene21535:CDS |
| ATGGCAGCAACGACTGGAAG+GGG | 0.541567 | 5.3:+6206371 | MS.gene21535:CDS |
| ACCTAAATGGGTTGATGAGG+TGG | 0.548455 | 5.3:+6206588 | MS.gene21535:CDS |
| ACTAGCCCTATTGGTGGCCG+AGG | 0.548502 | 5.3:+6206987 | MS.gene21535:CDS |
| ATTCATGAAGATGATGGACT+AGG | 0.548753 | 5.3:-6207570 | None:intergenic |
| AGGTAATCCAATTGATGATG+TGG | 0.548802 | 5.3:-6206569 | None:intergenic |
| GATATTGTGATATCTCTTCG+TGG | 0.549518 | 5.3:+6206706 | MS.gene21535:CDS |
| TCCAACCCAGCTAGAACGCT+GGG | 0.550701 | 5.3:-6206632 | None:intergenic |
| TTCAGCCCATTCAAGACAAG+TGG | 0.553127 | 5.3:-6206734 | None:intergenic |
| GGACGCTCAGACTCAGACTC+AGG | 0.556991 | 5.3:+6206789 | MS.gene21535:CDS |
| CGCCGGAGGTTGTCATCCAA+GGG | 0.557417 | 5.3:-6206403 | None:intergenic |
| ACAACAAGCTATGTCAGCAT+CGG | 0.563181 | 5.3:-6207319 | None:intergenic |
| CGGGCCAAACGTGCCACCCG+TGG | 0.563469 | 5.3:+6207023 | MS.gene21535:CDS |
| TCATGGCAGCAACGACTGGA+AGG | 0.564182 | 5.3:+6206369 | MS.gene21535:CDS |
| CGCCTTATTGAACTTTACAA+AGG | 0.564214 | 5.3:+6206919 | MS.gene21535:CDS |
| ATTCTCCAAGAAATATCATC+GGG | 0.566867 | 5.3:+6206329 | MS.gene21535:CDS |
| AGCTTGTTGTCATGATCTAG+AGG | 0.571065 | 5.3:+6207332 | MS.gene21535:CDS |
| GCCGAGGAAATTAGCACGTG+CGG | 0.571449 | 5.3:+6207003 | MS.gene21535:CDS |
| TGATGTGGCATACAGACTCT+TGG | 0.573169 | 5.3:-6206554 | None:intergenic |
| TAAGCGTTTCAGGTGGCGGG+GGG | 0.576225 | 5.3:-6206278 | None:intergenic |
| GGGTGCTTCAGTTGTTGACA+TGG | 0.576279 | 5.3:-6206488 | None:intergenic |
| CCGAGGAAATTAGCACGTGC+GGG | 0.576932 | 5.3:+6207004 | MS.gene21535:CDS |
| TCATTTGGCGGACCTCGTGT+TGG | 0.577348 | 5.3:+6207054 | MS.gene21535:CDS |
| CATATCTAACAACCTCTCGC+CGG | 0.581786 | 5.3:-6206420 | None:intergenic |
| CATGGCAGCAACGACTGGAA+GGG | 0.583943 | 5.3:+6206370 | MS.gene21535:CDS |
| AATTTGTGAAGGTTGTGAGC+AGG | 0.587076 | 5.3:-6205977 | None:intergenic |
| GCCAAGGCTATGGCCGGTAA+CGG | 0.591069 | 5.3:-6206959 | None:intergenic |
| TATTCTCCAAGAAATATCAT+CGG | 0.595846 | 5.3:+6206328 | MS.gene21535:CDS |
| AACGACACCTCTTGATGATG+GGG | 0.596360 | 5.3:-6206508 | None:intergenic |
| AGAAAGTGGAGGGAATATCA+TGG | 0.601338 | 5.3:+6206352 | MS.gene21535:CDS |
| TTGGTTGATGATGATTCCAA+AGG | 0.613659 | 5.3:-6206139 | None:intergenic |
| AGGTGGCACCAGATCTTGGT+TGG | 0.618491 | 5.3:+6206605 | MS.gene21535:CDS |
| GGATGACAACCTCCGGCGAG+AGG | 0.618831 | 5.3:+6206408 | MS.gene21535:CDS |
| CAGACTCAGACTCAGGCACA+AGG | 0.619166 | 5.3:+6206796 | MS.gene21535:CDS |
| TTAGTGCTTGAAATTTGTGA+AGG | 0.626013 | 5.3:-6205988 | None:intergenic |
| TCAAAGCCCAAAGTTGAGTG+TGG | 0.627127 | 5.3:+6206823 | MS.gene21535:CDS |
| ACAACAAGCAGCCATAGACA+TGG | 0.628855 | 5.3:-6207547 | None:intergenic |
| GCGTCGCACTTCTTCAACAA+CGG | 0.630718 | 5.3:-6206899 | None:intergenic |
| TTGTCTAATCTTAGTAGACA+GGG | 0.632883 | 5.3:+6207522 | MS.gene21535:CDS |
| ATCTAACAACCTCTCGCCGG+AGG | 0.634684 | 5.3:-6206417 | None:intergenic |
| TGAAGCACCCCATCATCAAG+AGG | 0.635362 | 5.3:+6206501 | MS.gene21535:CDS |
| TGTCGCGCCAAGGCTATGGC+CGG | 0.642253 | 5.3:-6206965 | None:intergenic |
| ACAGCGTCTTCTTTCCAAGA+CGG | 0.643880 | 5.3:+6206300 | MS.gene21535:CDS |
| TCTGTTAATCTTAATAGACA+AGG | 0.646433 | 5.3:+6207492 | MS.gene21535:CDS |
| TATCATCGGGAGAAAGTGGA+GGG | 0.648802 | 5.3:+6206342 | MS.gene21535:CDS |
| ATTACCTAAATGGGTTGATG+AGG | 0.663925 | 5.3:+6206585 | MS.gene21535:CDS |
| ATATCATCGGGAGAAAGTGG+AGG | 0.666029 | 5.3:+6206341 | MS.gene21535:CDS |
| ATAGGGCTAGTGTCGCGCCA+AGG | 0.667674 | 5.3:-6206975 | None:intergenic |
| CCAATTAGTCGATTTACCCG+AGG | 0.667868 | 5.3:+6206768 | MS.gene21535:CDS |
| GACGCTGTAAGCGTTTCAGG+TGG | 0.674999 | 5.3:-6206285 | None:intergenic |
| GCGTCTTCTTTCCAAGACGG+CGG | 0.680773 | 5.3:+6206303 | MS.gene21535:CDS |
| CCAACATGTGAATATCCCAA+AGG | 0.681313 | 5.3:-6207252 | None:intergenic |
| GAGCTTGTACAAGACAAAAG+GGG | 0.708452 | 5.3:+6206852 | MS.gene21535:CDS |
| CACACAAGACGTGATTACAA+GGG | 0.729372 | 5.3:+6207137 | MS.gene21535:CDS |
| AAGGCTCTGTTACCAACACG+AGG | 0.743509 | 5.3:-6207066 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAGTTGTATACTAGTA+AGG | + | chr5.3:6207458-6207477 | MS.gene21535:CDS | 20.0% |
| !! | AGAAAAAATTAAGAATTCCA+AGG | + | chr5.3:6207191-6207210 | MS.gene21535:CDS | 20.0% |
| ! | AAAAGATGAAGGTTGTTTAA+TGG | - | chr5.3:6206044-6206063 | None:intergenic | 25.0% |
| ! | AAAGGAAAAAACTTTCTTGA+AGG | + | chr5.3:6206205-6206224 | MS.gene21535:CDS | 25.0% |
| ! | ATCAATTGGATTACCTAAAT+GGG | + | chr5.3:6206576-6206595 | MS.gene21535:CDS | 25.0% |
| ! | ATCCAAAAAAGTGACAAAAA+AGG | + | chr5.3:6206187-6206206 | MS.gene21535:CDS | 25.0% |
| ! | TATTCTCCAAGAAATATCAT+CGG | + | chr5.3:6206328-6206347 | MS.gene21535:CDS | 25.0% |
| ! | TCTGTTAATCTTAATAGACA+AGG | + | chr5.3:6207492-6207511 | MS.gene21535:CDS | 25.0% |
| !! | GTTACTTTATAAGATCTTTC+CGG | - | chr5.3:6206535-6206554 | None:intergenic | 25.0% |
| !!! | TTCCTTTTTTGTCACTTTTT+TGG | - | chr5.3:6206192-6206211 | None:intergenic | 25.0% |
| !!! | TTTTTGTCACTTTTTTGGAT+AGG | - | chr5.3:6206187-6206206 | None:intergenic | 25.0% |
| AAAAGAGAGATTGCAAGAAT+GGG | + | chr5.3:6206676-6206695 | MS.gene21535:CDS | 30.0% | |
| AAACCAATAACAACTCATCT+AGG | + | chr5.3:6206074-6206093 | MS.gene21535:CDS | 30.0% | |
| AGAGGCTTATTTACATTTAG+TGG | + | chr5.3:6207350-6207369 | MS.gene21535:CDS | 30.0% | |
| ATAAATCCACACTCAACTTT+GGG | - | chr5.3:6206832-6206851 | None:intergenic | 30.0% | |
| ATTCTCCAAGAAATATCATC+GGG | + | chr5.3:6206329-6206348 | MS.gene21535:CDS | 30.0% | |
| CATCAATTGGATTACCTAAA+TGG | + | chr5.3:6206575-6206594 | MS.gene21535:CDS | 30.0% | |
| GCTTATTTACATTTAGTGGA+TGG | + | chr5.3:6207354-6207373 | MS.gene21535:CDS | 30.0% | |
| GTTGTTTAATGGAAGAAGAA+TGG | - | chr5.3:6206033-6206052 | None:intergenic | 30.0% | |
| TAAAAGAGAGATTGCAAGAA+TGG | + | chr5.3:6206675-6206694 | MS.gene21535:CDS | 30.0% | |
| TAACCTAGATGAGTTGTTAT+TGG | - | chr5.3:6206080-6206099 | None:intergenic | 30.0% | |
| TTAGTGCTTGAAATTTGTGA+AGG | - | chr5.3:6205991-6206010 | None:intergenic | 30.0% | |
| TTGTATACTAGTAAGGCTAA+AGG | + | chr5.3:6207465-6207484 | MS.gene21535:CDS | 30.0% | |
| TTGTCTAATCTTAGTAGACA+GGG | + | chr5.3:6207522-6207541 | MS.gene21535:CDS | 30.0% | |
| !!! | CTTTTTTGGATAGGTTGTAT+TGG | - | chr5.3:6206178-6206197 | None:intergenic | 30.0% |
| AACTTCCACTTGTCTTGAAT+GGG | + | chr5.3:6206729-6206748 | MS.gene21535:CDS | 35.0% | |
| AAGAATTCCAAGGTTGTTAG+TGG | + | chr5.3:6207201-6207220 | MS.gene21535:CDS | 35.0% | |
| ATGAGCTTGTACAAGACAAA+AGG | + | chr5.3:6206850-6206869 | MS.gene21535:CDS | 35.0% | |
| CATAAATCCACACTCAACTT+TGG | - | chr5.3:6206833-6206852 | None:intergenic | 35.0% | |
| CGCCTTTGTAAAGTTCAATA+AGG | - | chr5.3:6206924-6206943 | None:intergenic | 35.0% | |
| CTTGTCTAATCTTAGTAGAC+AGG | + | chr5.3:6207521-6207540 | MS.gene21535:CDS | 35.0% | |
| GAATTAAGCATAACCGTTAC+CGG | + | chr5.3:6206946-6206965 | MS.gene21535:CDS | 35.0% | |
| GACTTAACCTAGCAAAACTA+TGG | + | chr5.3:6206227-6206246 | MS.gene21535:CDS | 35.0% | |
| GATATTGTGATATCTCTTCG+TGG | + | chr5.3:6206706-6206725 | MS.gene21535:CDS | 35.0% | |
| GGTTGTTAGATATGGTGATT+TGG | + | chr5.3:6206429-6206448 | MS.gene21535:CDS | 35.0% | |
| TAGTGGAGTTGTTGATATGT+TGG | + | chr5.3:6207218-6207237 | MS.gene21535:CDS | 35.0% | |
| TCCTCGCTTAAAAATATTCC+TGG | - | chr5.3:6207165-6207184 | None:intergenic | 35.0% | |
| TGAAGAGAAGCAAAAGATGA+AGG | - | chr5.3:6206055-6206074 | None:intergenic | 35.0% | |
| TGAGCTTGTACAAGACAAAA+GGG | + | chr5.3:6206851-6206870 | MS.gene21535:CDS | 35.0% | |
| TTTCTCCCGATGATATTTCT+TGG | - | chr5.3:6206337-6206356 | None:intergenic | 35.0% | |
| ! | ACATTTAGTGGATGGATACA+TGG | + | chr5.3:6207362-6207381 | MS.gene21535:CDS | 35.0% |
| ! | ATGATGATTCCAAAGGCTTT+TGG | - | chr5.3:6206135-6206154 | None:intergenic | 35.0% |
| ! | CGCCTTATTGAACTTTACAA+AGG | + | chr5.3:6206919-6206938 | MS.gene21535:CDS | 35.0% |
| ! | TATTGGTTGTTGAAACTGGT+TGG | - | chr5.3:6206161-6206180 | None:intergenic | 35.0% |
| !! | AGGTAATCCAATTGATGATG+TGG | - | chr5.3:6206572-6206591 | None:intergenic | 35.0% |
| !! | ATTACCTAAATGGGTTGATG+AGG | + | chr5.3:6206585-6206604 | MS.gene21535:CDS | 35.0% |
| !! | CTCTTAGCATTAGCTCTAAA+TGG | - | chr5.3:6207399-6207418 | None:intergenic | 35.0% |
| !! | CTTTTCAAGATGTTCTCCAA+AGG | - | chr5.3:6207088-6207107 | None:intergenic | 35.0% |
| !! | GTTGTATTGGTTGTTGAAAC+TGG | - | chr5.3:6206165-6206184 | None:intergenic | 35.0% |
| !! | TTGGTTGATGATGATTCCAA+AGG | - | chr5.3:6206142-6206161 | None:intergenic | 35.0% |
| AACAACTCCACTAACAACCT+TGG | - | chr5.3:6207211-6207230 | None:intergenic | 40.0% | |
| AATTTGTGAAGGTTGTGAGC+AGG | - | chr5.3:6205980-6205999 | None:intergenic | 40.0% | |
| ACAACAAGCTATGTCAGCAT+CGG | - | chr5.3:6207322-6207341 | None:intergenic | 40.0% | |
| ACACACAAGACGTGATTACA+AGG | + | chr5.3:6207136-6207155 | MS.gene21535:CDS | 40.0% | |
| AGAAAGTGGAGGGAATATCA+TGG | + | chr5.3:6206352-6206371 | MS.gene21535:CDS | 40.0% | |
| CACACAAGACGTGATTACAA+GGG | + | chr5.3:6207137-6207156 | MS.gene21535:CDS | 40.0% | |
| CCAAATGAAAAAACAGCCAC+GGG | - | chr5.3:6207042-6207061 | None:intergenic | 40.0% | |
| CCAACATGTGAATATCCCAA+AGG | - | chr5.3:6207255-6207274 | None:intergenic | 40.0% | |
| CTCGGGTAAATCGACTAATT+GGG | - | chr5.3:6206770-6206789 | None:intergenic | 40.0% | |
| CTGTATGCCACATCATCAAT+TGG | + | chr5.3:6206562-6206581 | MS.gene21535:CDS | 40.0% | |
| GAAATATCATCGGGAGAAAG+TGG | + | chr5.3:6206338-6206357 | MS.gene21535:CDS | 40.0% | |
| GAACTTCCACTTGTCTTGAA+TGG | + | chr5.3:6206728-6206747 | MS.gene21535:CDS | 40.0% | |
| GAGCTTGTACAAGACAAAAG+GGG | + | chr5.3:6206852-6206871 | MS.gene21535:CDS | 40.0% | |
| GCCAAATGAAAAAACAGCCA+CGG | - | chr5.3:6207043-6207062 | None:intergenic | 40.0% | |
| TTGGAAGAGAACACTCCTTT+GGG | + | chr5.3:6207237-6207256 | MS.gene21535:CDS | 40.0% | |
| ! | ACTCAACTTTGGGCTTTGAT+TGG | - | chr5.3:6206822-6206841 | None:intergenic | 40.0% |
| ! | GAAGAATGGCATCTGAAACT+AGG | - | chr5.3:6206019-6206038 | None:intergenic | 40.0% |
| ! | TCTTCAGCTGCTTTAATCTC+TGG | - | chr5.3:6206253-6206272 | None:intergenic | 40.0% |
| ! | TCTTGATAGCCAAAAGCCTT+TGG | + | chr5.3:6206123-6206142 | MS.gene21535:CDS | 40.0% |
| !! | AGCTTGTTGTCATGATCTAG+AGG | + | chr5.3:6207332-6207351 | MS.gene21535:CDS | 40.0% |
| !! | CCTTTGGGATATTCACATGT+TGG | + | chr5.3:6207252-6207271 | MS.gene21535:CDS | 40.0% |
| !! | GCCAGGAATATTTTTAAGCG+AGG | + | chr5.3:6207161-6207180 | MS.gene21535:CDS | 40.0% |
| !!! | GTGGCTGTTTTTTCATTTGG+CGG | + | chr5.3:6207042-6207061 | MS.gene21535:CDS | 40.0% |
| AATATCATGGCAGCAACGAC+TGG | + | chr5.3:6206365-6206384 | MS.gene21535:CDS | 45.0% | |
| AATGAAAAAACAGCCACGGG+TGG | - | chr5.3:6207039-6207058 | None:intergenic | 45.0% | |
| AATTTCCTCGGCCACCAATA+GGG | - | chr5.3:6206995-6207014 | None:intergenic | 45.0% | |
| ACAACAAGCAGCCATAGACA+TGG | - | chr5.3:6207550-6207569 | None:intergenic | 45.0% | |
| ACAGCGTCTTCTTTCCAAGA+CGG | + | chr5.3:6206300-6206319 | MS.gene21535:CDS | 45.0% | |
| ATATCATCGGGAGAAAGTGG+AGG | + | chr5.3:6206341-6206360 | MS.gene21535:CDS | 45.0% | |
| CAACAAGCTATGTCAGCATC+GGG | - | chr5.3:6207321-6207340 | None:intergenic | 45.0% | |
| CATATCTAACAACCTCTCGC+CGG | - | chr5.3:6206423-6206442 | None:intergenic | 45.0% | |
| CCTCGGGTAAATCGACTAAT+TGG | - | chr5.3:6206771-6206790 | None:intergenic | 45.0% | |
| GTTGGAAGAGAACACTCCTT+TGG | + | chr5.3:6207236-6207255 | MS.gene21535:CDS | 45.0% | |
| TAATTTCCTCGGCCACCAAT+AGG | - | chr5.3:6206996-6207015 | None:intergenic | 45.0% | |
| TAGACAGGGATCCATGTCTA+TGG | + | chr5.3:6207536-6207555 | MS.gene21535:CDS | 45.0% | |
| TATCATCGGGAGAAAGTGGA+GGG | + | chr5.3:6206342-6206361 | MS.gene21535:CDS | 45.0% | |
| TCAAAGCCCAAAGTTGAGTG+TGG | + | chr5.3:6206823-6206842 | MS.gene21535:CDS | 45.0% | |
| TGATGTGGCATACAGACTCT+TGG | - | chr5.3:6206557-6206576 | None:intergenic | 45.0% | |
| TTCAGCCCATTCAAGACAAG+TGG | - | chr5.3:6206737-6206756 | None:intergenic | 45.0% | |
| ! | CAACGACACCTCTTGATGAT+GGG | - | chr5.3:6206512-6206531 | None:intergenic | 45.0% |
| ! | CACTTCTTCAACAACGGACT+CGG | - | chr5.3:6206896-6206915 | None:intergenic | 45.0% |
| ! | CCAATTAGTCGATTTACCCG+AGG | + | chr5.3:6206768-6206787 | MS.gene21535:CDS | 45.0% |
| !! | AACGACACCTCTTGATGATG+GGG | - | chr5.3:6206511-6206530 | None:intergenic | 45.0% |
| !! | ACCTAAATGGGTTGATGAGG+TGG | + | chr5.3:6206588-6206607 | MS.gene21535:CDS | 45.0% |
| !! | TCTCTGGCCATAGTTTTGCT+AGG | - | chr5.3:6206237-6206256 | None:intergenic | 45.0% |
| !!! | CCCGTGGCTGTTTTTTCATT+TGG | + | chr5.3:6207039-6207058 | MS.gene21535:CDS | 45.0% |
| AAGGCTCTGTTACCAACACG+AGG | - | chr5.3:6207069-6207088 | None:intergenic | 50.0% | |
| ATCCCTTGGATGACAACCTC+CGG | + | chr5.3:6206401-6206420 | MS.gene21535:CDS | 50.0% | |
| CGGCGAGAGGTTGTTAGATA+TGG | + | chr5.3:6206421-6206440 | MS.gene21535:CDS | 50.0% | |
| GAAGACGCTGTAAGCGTTTC+AGG | - | chr5.3:6206291-6206310 | None:intergenic | 50.0% | |
| GAAGGGGATGTTAGATCCCT+TGG | + | chr5.3:6206387-6206406 | MS.gene21535:CDS | 50.0% | |
| GCAACGACACCTCTTGATGA+TGG | - | chr5.3:6206513-6206532 | None:intergenic | 50.0% | |
| GCCACCTCATCAACCCATTT+AGG | - | chr5.3:6206592-6206611 | None:intergenic | 50.0% | |
| GCGTCGCACTTCTTCAACAA+CGG | - | chr5.3:6206902-6206921 | None:intergenic | 50.0% | |
| GCTTCAGTTGTTGACATGGC+AGG | - | chr5.3:6206487-6206506 | None:intergenic | 50.0% | |
| GGGTGCTTCAGTTGTTGACA+TGG | - | chr5.3:6206491-6206510 | None:intergenic | 50.0% | |
| GTGAAGGTTGTGAGCAGGTA+AGG | - | chr5.3:6205975-6205994 | None:intergenic | 50.0% | |
| TGGGTCATCCAACCAAGATC+TGG | - | chr5.3:6206616-6206635 | None:intergenic | 50.0% | |
| ! | CTTCAACAACGGACTCGGAT+AGG | - | chr5.3:6206891-6206910 | None:intergenic | 50.0% |
| ! | TCAGCATCGGGCTTCAAGTA+AGG | - | chr5.3:6207309-6207328 | None:intergenic | 50.0% |
| ! | TGAAGCACCCCATCATCAAG+AGG | + | chr5.3:6206501-6206520 | MS.gene21535:CDS | 50.0% |
| !! | CGTGTTGGTAACAGAGCCTT+TGG | + | chr5.3:6207069-6207088 | MS.gene21535:CDS | 50.0% |
| !! | TCATCAAGAGGTGTCGTTGC+CGG | + | chr5.3:6206513-6206532 | MS.gene21535:CDS | 50.0% |
| AGGTGGCACCAGATCTTGGT+TGG | + | chr5.3:6206605-6206624 | MS.gene21535:CDS | 55.0% | |
| AGTCTGAGTCTGAGCGTCCT+CGG | - | chr5.3:6206788-6206807 | None:intergenic | 55.0% | |
| ATCCAACCCAGCTAGAACGC+TGG | - | chr5.3:6206636-6206655 | None:intergenic | 55.0% | |
| ATCTAACAACCTCTCGCCGG+AGG | - | chr5.3:6206420-6206439 | None:intergenic | 55.0% | |
| ATGGCAGCAACGACTGGAAG+GGG | + | chr5.3:6206371-6206390 | MS.gene21535:CDS | 55.0% | |
| CAGACTCAGACTCAGGCACA+AGG | + | chr5.3:6206796-6206815 | MS.gene21535:CDS | 55.0% | |
| CATGGCAGCAACGACTGGAA+GGG | + | chr5.3:6206370-6206389 | MS.gene21535:CDS | 55.0% | |
| CCCGCACGTGCTAATTTCCT+CGG | - | chr5.3:6207007-6207026 | None:intergenic | 55.0% | |
| CCGAGGAAATTAGCACGTGC+GGG | + | chr5.3:6207004-6207023 | MS.gene21535:CDS | 55.0% | |
| GACGCTGTAAGCGTTTCAGG+TGG | - | chr5.3:6206288-6206307 | None:intergenic | 55.0% | |
| GATGAGGTGGCACCAGATCT+TGG | + | chr5.3:6206601-6206620 | MS.gene21535:CDS | 55.0% | |
| GCCGAGGAAATTAGCACGTG+CGG | + | chr5.3:6207003-6207022 | MS.gene21535:CDS | 55.0% | |
| GCGTCTTCTTTCCAAGACGG+CGG | + | chr5.3:6206303-6206322 | MS.gene21535:CDS | 55.0% | |
| TCATGGCAGCAACGACTGGA+AGG | + | chr5.3:6206369-6206388 | MS.gene21535:CDS | 55.0% | |
| TCCAACCCAGCTAGAACGCT+GGG | - | chr5.3:6206635-6206654 | None:intergenic | 55.0% | |
| TGGAGAATACTCCGCCGTCT+TGG | - | chr5.3:6206317-6206336 | None:intergenic | 55.0% | |
| ! | CTGTAAGCGTTTCAGGTGGC+GGG | - | chr5.3:6206284-6206303 | None:intergenic | 55.0% |
| ! | GATGACCCAGCGTTCTAGCT+GGG | + | chr5.3:6206627-6206646 | MS.gene21535:CDS | 55.0% |
| ! | GCTGTAAGCGTTTCAGGTGG+CGG | - | chr5.3:6206285-6206304 | None:intergenic | 55.0% |
| ! | TCATTTGGCGGACCTCGTGT+TGG | + | chr5.3:6207054-6207073 | MS.gene21535:CDS | 55.0% |
| ! | TGTAAGCGTTTCAGGTGGCG+GGG | - | chr5.3:6206283-6206302 | None:intergenic | 55.0% |
| !! | GACGTGATTACAAGGGTGCC+AGG | + | chr5.3:6207144-6207163 | MS.gene21535:CDS | 55.0% |
| ACCGTTACCGGCCATAGCCT+TGG | + | chr5.3:6206958-6206977 | MS.gene21535:CDS | 60.0% | |
| CGCCGGAGGTTGTCATCCAA+GGG | - | chr5.3:6206406-6206425 | None:intergenic | 60.0% | |
| CGCGACACTAGCCCTATTGG+TGG | + | chr5.3:6206981-6207000 | MS.gene21535:CDS | 60.0% | |
| CTAGTGTCGCGCCAAGGCTA+TGG | - | chr5.3:6206972-6206991 | None:intergenic | 60.0% | |
| GCCAAGGCTATGGCCGGTAA+CGG | - | chr5.3:6206962-6206981 | None:intergenic | 60.0% | |
| GGACGCTCAGACTCAGACTC+AGG | + | chr5.3:6206789-6206808 | MS.gene21535:CDS | 60.0% | |
| GGATGACCCAGCGTTCTAGC+TGG | + | chr5.3:6206626-6206645 | MS.gene21535:CDS | 60.0% | |
| GTCTGAGTCTGAGCGTCCTC+GGG | - | chr5.3:6206787-6206806 | None:intergenic | 60.0% | |
| TCGCCGGAGGTTGTCATCCA+AGG | - | chr5.3:6206407-6206426 | None:intergenic | 60.0% | |
| TGGCGCGACACTAGCCCTAT+TGG | + | chr5.3:6206978-6206997 | MS.gene21535:CDS | 60.0% | |
| ! | ACCCAGCGTTCTAGCTGGGT+TGG | + | chr5.3:6206631-6206650 | MS.gene21535:CDS | 60.0% |
| ! | ACTAGCCCTATTGGTGGCCG+AGG | + | chr5.3:6206987-6207006 | MS.gene21535:CDS | 60.0% |
| ! | ATAGGGCTAGTGTCGCGCCA+AGG | - | chr5.3:6206978-6206997 | None:intergenic | 60.0% |
| ! | GTAAGCGTTTCAGGTGGCGG+GGG | - | chr5.3:6206282-6206301 | None:intergenic | 60.0% |
| !! | TAAGCGTTTCAGGTGGCGGG+GGG | - | chr5.3:6206281-6206300 | None:intergenic | 60.0% |
| ACAGCCACGGGTGGCACGTT+TGG | - | chr5.3:6207030-6207049 | None:intergenic | 65.0% | |
| GGATGACAACCTCCGGCGAG+AGG | + | chr5.3:6206408-6206427 | MS.gene21535:CDS | 65.0% | |
| TGTCGCGCCAAGGCTATGGC+CGG | - | chr5.3:6206968-6206987 | None:intergenic | 65.0% | |
| CGGGCCAAACGTGCCACCCG+TGG | + | chr5.3:6207023-6207042 | MS.gene21535:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 6205950 | 6207590 | 6205950 | ID=MS.gene21535 |
| chr5.3 | mRNA | 6205950 | 6207590 | 6205950 | ID=MS.gene21535.t1;Parent=MS.gene21535 |
| chr5.3 | exon | 6205950 | 6207590 | 6205950 | ID=MS.gene21535.t1.exon1;Parent=MS.gene21535.t1 |
| chr5.3 | CDS | 6205950 | 6207590 | 6205950 | ID=cds.MS.gene21535.t1;Parent=MS.gene21535.t1 |
| Gene Sequence |
| Protein sequence |
