Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21707.t1 | XP_003610916.1 | 99.1 | 113 | 1 | 0 | 1 | 113 | 1 | 113 | 7.90E-59 | 236.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21707.t1 | Q9T0G2 | 91.1 | 112 | 10 | 0 | 1 | 112 | 1 | 112 | 3.7e-57 | 221.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21707.t1 | G7K7W9 | 99.1 | 113 | 1 | 0 | 1 | 113 | 1 | 113 | 5.7e-59 | 236.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058150 | MS.gene21707 | 0.99516 | 1.41E-213 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene074110 | MS.gene21707 | PPI |
| MS.gene21707 | MS.gene074111 | PPI |
| MS.gene47123 | MS.gene21707 | PPI |
| MS.gene55480 | MS.gene21707 | PPI |
| MS.gene21707 | MS.gene55486 | PPI |
| MS.gene47121 | MS.gene21707 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21707.t1 | MTR_5g008460 | 99.115 | 113 | 1 | 0 | 1 | 113 | 1 | 113 | 7.94e-82 | 234 |
| MS.gene21707.t1 | MTR_7g080803 | 89.381 | 113 | 12 | 0 | 1 | 113 | 1 | 113 | 8.10e-75 | 217 |
| MS.gene21707.t1 | MTR_7g080793 | 70.370 | 108 | 31 | 1 | 1 | 108 | 1 | 107 | 4.43e-52 | 159 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21707.t1 | AT4G10040 | 91.071 | 112 | 10 | 0 | 1 | 112 | 1 | 112 | 1.79e-75 | 219 |
| MS.gene21707.t1 | AT1G22840 | 88.393 | 112 | 13 | 0 | 1 | 112 | 1 | 112 | 8.81e-73 | 212 |
| MS.gene21707.t1 | AT1G22840 | 84.524 | 84 | 13 | 0 | 1 | 84 | 1 | 84 | 2.06e-50 | 155 |
Find 41 sgRNAs with CRISPR-Local
Find 92 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCAATCTGAATGGTTTGTT+TGG | 0.305862 | 5.2:+2411838 | MS.gene21707:CDS |
| AATAAGATCAGCACGTTCTT+GGG | 0.344346 | 5.2:-2412486 | None:intergenic |
| CAATAAGATCAGCACGTTCT+TGG | 0.348957 | 5.2:-2412487 | None:intergenic |
| ACAAGAACATGGCTGTGAAT+TGG | 0.349587 | 5.2:+2411905 | MS.gene21707:CDS |
| TTATTTGCTTAACCCCAAAA+AGG | 0.352975 | 5.2:+2411948 | MS.gene21707:CDS |
| CAAGAACATGGCTGTGAATT+GGG | 0.354427 | 5.2:+2411906 | MS.gene21707:CDS |
| AGCTGCCGAGTAGGAATATC+CGG | 0.375428 | 5.2:-2411881 | None:intergenic |
| GGTTTGTTTGGAAGGCAATC+AGG | 0.375977 | 5.2:+2411850 | MS.gene21707:CDS |
| GCTCTTGTGCAGTATATTCC+AGG | 0.394249 | 5.2:+2412431 | MS.gene21707:intron |
| GGTGTGACACTGAGCGCATT+TGG | 0.415295 | 5.2:-2410765 | None:intergenic |
| TTCTTGGGGTTTCTTTAGAC+CGG | 0.417289 | 5.2:-2412471 | None:intergenic |
| ATCTCCGGATTTGGCATCAC+CGG | 0.423980 | 5.2:-2410729 | None:intergenic |
| TCCAAACAAACCATTCAGAT+TGG | 0.424075 | 5.2:-2411839 | None:intergenic |
| ATCTGAATGGTTTGTTTGGA+AGG | 0.441498 | 5.2:+2411842 | MS.gene21707:CDS |
| TCAGTGTCACACCGTCGATA+AGG | 0.441684 | 5.2:+2410775 | MS.gene21707:CDS |
| CAGTGTCACACCGTCGATAA+GGG | 0.454494 | 5.2:+2410776 | MS.gene21707:CDS |
| GGGACCAAGATGGTATTTCC+CGG | 0.471291 | 5.2:+2412452 | MS.gene21707:CDS |
| CCTCCCGGTGATGCCAAATC+CGG | 0.475381 | 5.2:+2410725 | MS.gene21707:CDS |
| CCAAACAAACCATTCAGATT+GGG | 0.484079 | 5.2:-2411838 | None:intergenic |
| GATCTAACCTTGTTTGTGAC+CGG | 0.503624 | 5.2:-2410801 | None:intergenic |
| AGGCAATCAGGCACTACTCC+CGG | 0.508362 | 5.2:+2411862 | MS.gene21707:CDS |
| GGAAATACCATCTTGGTCCC+TGG | 0.530773 | 5.2:-2412449 | None:intergenic |
| TAGACCGGGAAATACCATCT+TGG | 0.536477 | 5.2:-2412456 | None:intergenic |
| ATCTCAGGACCCAATCTGAA+TGG | 0.539851 | 5.2:+2411829 | MS.gene21707:intron |
| TCTCCGGATTTGGCATCACC+GGG | 0.561033 | 5.2:-2410728 | None:intergenic |
| CTCTTGTGCAGTATATTCCA+GGG | 0.564315 | 5.2:+2412432 | MS.gene21707:intron |
| TCGTTTGAACAAGCACCTCC+CGG | 0.572771 | 5.2:+2410710 | MS.gene21707:CDS |
| GTTCTTGTTAGCTGCCGAGT+AGG | 0.576933 | 5.2:-2411890 | None:intergenic |
| TCTTGGGGTTTCTTTAGACC+GGG | 0.586837 | 5.2:-2412470 | None:intergenic |
| AAGGGTGCCGGTCACAAACA+AGG | 0.600344 | 5.2:+2410794 | MS.gene21707:CDS |
| CTCGGCAGCTAACAAGAACA+TGG | 0.601333 | 5.2:+2411894 | MS.gene21707:CDS |
| ATAAGATCAGCACGTTCTTG+GGG | 0.603518 | 5.2:-2412485 | None:intergenic |
| TGATCTTATTGCATATCTGA+AGG | 0.616592 | 5.2:+2412499 | MS.gene21707:CDS |
| CACACCGTCGATAAGGGTGC+CGG | 0.624319 | 5.2:+2410782 | MS.gene21707:CDS |
| TACTCCCGGATATTCCTACT+CGG | 0.628766 | 5.2:+2411876 | MS.gene21707:CDS |
| AATATATAATCACTGTGCAG+TGG | 0.632456 | 5.2:-2412525 | None:intergenic |
| GTGACCGGCACCCTTATCGA+CGG | 0.634194 | 5.2:-2410786 | None:intergenic |
| GCTGCCGAGTAGGAATATCC+GGG | 0.639197 | 5.2:-2411880 | None:intergenic |
| GTATATTCCAGGGACCAAGA+TGG | 0.661039 | 5.2:+2412442 | MS.gene21707:intron |
| GAACATGGCTGTGAATTGGG+AGG | 0.673762 | 5.2:+2411909 | MS.gene21707:CDS |
| CCGGATTTGGCATCACCGGG+AGG | 0.684893 | 5.2:-2410725 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATACTAATATAATATCAA+GGG | - | chr5.2:2411652-2411671 | None:intergenic | 10.0% |
| !!! | AATAATATTTAGTATTGATT+AGG | - | chr5.2:2412274-2412293 | None:intergenic | 10.0% |
| !! | AATCTCTATATCTATAATTT+AGG | + | chr5.2:2411711-2411730 | MS.gene21707:intron | 15.0% |
| !! | CATATACTAATATAATATCA+AGG | - | chr5.2:2411653-2411672 | None:intergenic | 15.0% |
| !! | TATTTCAATATTTGATTCAT+AGG | + | chr5.2:2411561-2411580 | MS.gene21707:intron | 15.0% |
| !!! | AAATAATTACATTTTCAATG+AGG | - | chr5.2:2411042-2411061 | None:intergenic | 15.0% |
| !!! | ATTTGTGTTTATTTTATTGT+TGG | + | chr5.2:2410949-2410968 | MS.gene21707:intron | 15.0% |
| !! | ATAAAAAAGCTATTGTTTGT+AGG | + | chr5.2:2411196-2411215 | MS.gene21707:intron | 20.0% |
| !! | ATCATCTAATATTATTGTTC+AGG | + | chr5.2:2411125-2411144 | MS.gene21707:intron | 20.0% |
| !! | GTGAAAATAATGTATAATCA+TGG | - | chr5.2:2410899-2410918 | None:intergenic | 20.0% |
| !! | TAAAAAAGCTATTGTTTGTA+GGG | + | chr5.2:2411197-2411216 | MS.gene21707:intron | 20.0% |
| !! | TAGTACATAATATTGTGAAA+TGG | - | chr5.2:2411417-2411436 | None:intergenic | 20.0% |
| !! | TATTTGATTCATAGGTTTAT+TGG | + | chr5.2:2411569-2411588 | MS.gene21707:intron | 20.0% |
| !!! | AATATAATATCAAGGGTATA+AGG | - | chr5.2:2411645-2411664 | None:intergenic | 20.0% |
| !!! | TCTAGTATTTCTATATTGTA+TGG | + | chr5.2:2411981-2412000 | MS.gene21707:intron | 20.0% |
| !!! | TTGTTTTTCTTTTCGTTTAT+AGG | + | chr5.2:2412308-2412327 | MS.gene21707:intron | 20.0% |
| ! | AATAAGATTTACAGCTTAGT+TGG | + | chr5.2:2412013-2412032 | MS.gene21707:intron | 25.0% |
| ! | ATTAGCATATTACCACAATA+TGG | - | chr5.2:2412353-2412372 | None:intergenic | 25.0% |
| ! | GAATAACGTGATTATCTATT+TGG | + | chr5.2:2411782-2411801 | MS.gene21707:intron | 25.0% |
| ! | GATTTATGATTCAAGGAATT+TGG | + | chr5.2:2411094-2411113 | MS.gene21707:intron | 25.0% |
| ! | TCAGGATAATATTTGATTGA+TGG | + | chr5.2:2411143-2411162 | MS.gene21707:intron | 25.0% |
| !! | AGATTGTATTGTACCTTTTT+GGG | - | chr5.2:2411964-2411983 | None:intergenic | 25.0% |
| !! | TAGATTGTATTGTACCTTTT+TGG | - | chr5.2:2411965-2411984 | None:intergenic | 25.0% |
| !!! | TTTCTAAGATTTCCATATTG+TGG | + | chr5.2:2412338-2412357 | MS.gene21707:intron | 25.0% |
| AAACGAAAAGAAAAACAAGC+AGG | - | chr5.2:2412306-2412325 | None:intergenic | 30.0% | |
| AACGAAAAGAAAAACAAGCA+GGG | - | chr5.2:2412305-2412324 | None:intergenic | 30.0% | |
| CATAGATCAGTAACAAATTG+TGG | - | chr5.2:2412408-2412427 | None:intergenic | 30.0% | |
| TGATCTTATTGCATATCTGA+AGG | + | chr5.2:2412499-2412518 | MS.gene21707:CDS | 30.0% | |
| TTAGATCAGTTTCTCATATG+TGG | + | chr5.2:2410817-2410836 | MS.gene21707:intron | 30.0% | |
| TTATTTGCTTAACCCCAAAA+AGG | + | chr5.2:2411948-2411967 | MS.gene21707:CDS | 30.0% | |
| ! | AAAGATTTTATCTCCGGATT+TGG | - | chr5.2:2410741-2410760 | None:intergenic | 30.0% |
| ! | GATTGTATTGTACCTTTTTG+GGG | - | chr5.2:2411963-2411982 | None:intergenic | 30.0% |
| !! | TAGGTTTATTGGTGTTGTAA+TGG | + | chr5.2:2411580-2411599 | MS.gene21707:intron | 30.0% |
| !! | TGCTGTTGATTTATGATTCA+AGG | + | chr5.2:2411087-2411106 | MS.gene21707:intron | 30.0% |
| AACACAAATTGCATAGCATG+TGG | - | chr5.2:2410938-2410957 | None:intergenic | 35.0% | |
| AATAAGATCAGCACGTTCTT+GGG | - | chr5.2:2412489-2412508 | None:intergenic | 35.0% | |
| AATTGCATAGCATGTGGAAA+TGG | - | chr5.2:2410932-2410951 | None:intergenic | 35.0% | |
| ACCTTCACATATAACAAGCA+AGG | + | chr5.2:2412160-2412179 | MS.gene21707:intron | 35.0% | |
| AGATCAGTAACAAATTGTGG+TGG | - | chr5.2:2412405-2412424 | None:intergenic | 35.0% | |
| AGCAGTATTATTGTCAATCG+TGG | + | chr5.2:2411328-2411347 | MS.gene21707:intron | 35.0% | |
| AGTTGTAAGCTAATCCAAAC+TGG | + | chr5.2:2412234-2412253 | MS.gene21707:intron | 35.0% | |
| ATGTGGAAATGGAACGATTA+AGG | - | chr5.2:2410921-2410940 | None:intergenic | 35.0% | |
| CCAAACAAACCATTCAGATT+GGG | - | chr5.2:2411841-2411860 | None:intergenic | 35.0% | |
| GCAGTATTATTGTCAATCGT+GGG | + | chr5.2:2411329-2411348 | MS.gene21707:intron | 35.0% | |
| TCCAAACAAACCATTCAGAT+TGG | - | chr5.2:2411842-2411861 | None:intergenic | 35.0% | |
| TTGATTAGGAGTGAAGCATA+TGG | - | chr5.2:2412260-2412279 | None:intergenic | 35.0% | |
| ! | GGTTCGAAAGATTTTATCTC+CGG | - | chr5.2:2410747-2410766 | None:intergenic | 35.0% |
| ! | GTTTTTCATTACTCCTTTGC+TGG | + | chr5.2:2411672-2411691 | MS.gene21707:intron | 35.0% |
| !! | AGCACAATAAGTTTGTTGCA+CGG | - | chr5.2:2410981-2411000 | None:intergenic | 35.0% |
| !! | ATCTGAATGGTTTGTTTGGA+AGG | + | chr5.2:2411842-2411861 | MS.gene21707:CDS | 35.0% |
| !! | TTGCTTGTTATATGTGAAGG+TGG | - | chr5.2:2412161-2412180 | None:intergenic | 35.0% |
| !! | TTTGCCTTGTTGATAATCTC+AGG | + | chr5.2:2411814-2411833 | MS.gene21707:intron | 35.0% |
| ACAAGAACATGGCTGTGAAT+TGG | + | chr5.2:2411905-2411924 | MS.gene21707:CDS | 40.0% | |
| ATAAGATCAGCACGTTCTTG+GGG | - | chr5.2:2412488-2412507 | None:intergenic | 40.0% | |
| CAAGAACATGGCTGTGAATT+GGG | + | chr5.2:2411906-2411925 | MS.gene21707:CDS | 40.0% | |
| CAATAAGATCAGCACGTTCT+TGG | - | chr5.2:2412490-2412509 | None:intergenic | 40.0% | |
| CTCTTGTGCAGTATATTCCA+GGG | + | chr5.2:2412432-2412451 | MS.gene21707:intron | 40.0% | |
| GTTATATGTGAAGGTGGCAA+TGG | - | chr5.2:2412155-2412174 | None:intergenic | 40.0% | |
| TTCTTGGGGTTTCTTTAGAC+CGG | - | chr5.2:2412474-2412493 | None:intergenic | 40.0% | |
| ! | GATCTAACCTTGTTTGTGAC+CGG | - | chr5.2:2410804-2410823 | None:intergenic | 40.0% |
| ! | TTATTGTCAATCGTGGGTTG+CGG | + | chr5.2:2411335-2411354 | MS.gene21707:intron | 40.0% |
| !! | CCCAATCTGAATGGTTTGTT+TGG | + | chr5.2:2411838-2411857 | MS.gene21707:CDS | 40.0% |
| !! | GCCTTGCTTGTTATATGTGA+AGG | - | chr5.2:2412164-2412183 | None:intergenic | 40.0% |
| AGTGAAGCATATGGCCAGTT+TGG | - | chr5.2:2412251-2412270 | None:intergenic | 45.0% | |
| ATCAAACTCAGTGCCAGCAA+AGG | - | chr5.2:2411688-2411707 | None:intergenic | 45.0% | |
| ATCTCAGGACCCAATCTGAA+TGG | + | chr5.2:2411829-2411848 | MS.gene21707:intron | 45.0% | |
| GCTCTTGTGCAGTATATTCC+AGG | + | chr5.2:2412431-2412450 | MS.gene21707:intron | 45.0% | |
| GGGTCCTGAGATTATCAACA+AGG | - | chr5.2:2411821-2411840 | None:intergenic | 45.0% | |
| GTATATTCCAGGGACCAAGA+TGG | + | chr5.2:2412442-2412461 | MS.gene21707:intron | 45.0% | |
| TACTCCCGGATATTCCTACT+CGG | + | chr5.2:2411876-2411895 | MS.gene21707:CDS | 45.0% | |
| TAGACCGGGAAATACCATCT+TGG | - | chr5.2:2412459-2412478 | None:intergenic | 45.0% | |
| TCTTGGGGTTTCTTTAGACC+GGG | - | chr5.2:2412473-2412492 | None:intergenic | 45.0% | |
| ! | GGTTTGTTTGGAAGGCAATC+AGG | + | chr5.2:2411850-2411869 | MS.gene21707:CDS | 45.0% |
| AGCTGCCGAGTAGGAATATC+CGG | - | chr5.2:2411884-2411903 | None:intergenic | 50.0% | |
| ATCTCCGGATTTGGCATCAC+CGG | - | chr5.2:2410732-2410751 | None:intergenic | 50.0% | |
| CAGTGTCACACCGTCGATAA+GGG | + | chr5.2:2410776-2410795 | MS.gene21707:CDS | 50.0% | |
| CTCGGCAGCTAACAAGAACA+TGG | + | chr5.2:2411894-2411913 | MS.gene21707:CDS | 50.0% | |
| GAACATGGCTGTGAATTGGG+AGG | + | chr5.2:2411909-2411928 | MS.gene21707:CDS | 50.0% | |
| GGAAATACCATCTTGGTCCC+TGG | - | chr5.2:2412452-2412471 | None:intergenic | 50.0% | |
| GGGACCAAGATGGTATTTCC+CGG | + | chr5.2:2412452-2412471 | MS.gene21707:CDS | 50.0% | |
| GTTCTTGTTAGCTGCCGAGT+AGG | - | chr5.2:2411893-2411912 | None:intergenic | 50.0% | |
| TCAGTGTCACACCGTCGATA+AGG | + | chr5.2:2410775-2410794 | MS.gene21707:CDS | 50.0% | |
| TCGTTTGAACAAGCACCTCC+CGG | + | chr5.2:2410710-2410729 | MS.gene21707:CDS | 50.0% | |
| AGGCAATCAGGCACTACTCC+CGG | + | chr5.2:2411862-2411881 | MS.gene21707:CDS | 55.0% | |
| GCTGCCGAGTAGGAATATCC+GGG | - | chr5.2:2411883-2411902 | None:intergenic | 55.0% | |
| GGTGTGACACTGAGCGCATT+TGG | - | chr5.2:2410768-2410787 | None:intergenic | 55.0% | |
| TCTCCGGATTTGGCATCACC+GGG | - | chr5.2:2410731-2410750 | None:intergenic | 55.0% | |
| !! | AAGGGTGCCGGTCACAAACA+AGG | + | chr5.2:2410794-2410813 | MS.gene21707:CDS | 55.0% |
| CCTCCCGGTGATGCCAAATC+CGG | + | chr5.2:2410725-2410744 | MS.gene21707:CDS | 60.0% | |
| GTGACCGGCACCCTTATCGA+CGG | - | chr5.2:2410789-2410808 | None:intergenic | 60.0% | |
| ! | CACACCGTCGATAAGGGTGC+CGG | + | chr5.2:2410782-2410801 | MS.gene21707:CDS | 60.0% |
| CCGGATTTGGCATCACCGGG+AGG | - | chr5.2:2410728-2410747 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 2410704 | 2412538 | 2410704 | ID=MS.gene21707 |
| chr5.2 | mRNA | 2410704 | 2412538 | 2410704 | ID=MS.gene21707.t1;Parent=MS.gene21707 |
| chr5.2 | exon | 2410704 | 2410815 | 2410704 | ID=MS.gene21707.t1.exon1;Parent=MS.gene21707.t1 |
| chr5.2 | CDS | 2410704 | 2410815 | 2410704 | ID=cds.MS.gene21707.t1;Parent=MS.gene21707.t1 |
| chr5.2 | exon | 2411836 | 2411969 | 2411836 | ID=MS.gene21707.t1.exon2;Parent=MS.gene21707.t1 |
| chr5.2 | CDS | 2411836 | 2411969 | 2411836 | ID=cds.MS.gene21707.t1;Parent=MS.gene21707.t1 |
| chr5.2 | exon | 2412443 | 2412538 | 2412443 | ID=MS.gene21707.t1.exon3;Parent=MS.gene21707.t1 |
| chr5.2 | CDS | 2412443 | 2412538 | 2412443 | ID=cds.MS.gene21707.t1;Parent=MS.gene21707.t1 |
| Gene Sequence |
| Protein sequence |
