Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058150.t1 | XP_003610916.1 | 99.1 | 113 | 1 | 0 | 1 | 113 | 1 | 113 | 7.90E-59 | 236.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058150.t1 | Q9T0G2 | 91.1 | 112 | 10 | 0 | 1 | 112 | 1 | 112 | 3.7e-57 | 221.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058150.t1 | G7K7W9 | 99.1 | 113 | 1 | 0 | 1 | 113 | 1 | 113 | 5.7e-59 | 236.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058150 | MS.gene21707 | 0.99516 | 1.41E-213 | -1.69E-46 |
| MS.gene058150 | MS.gene21918 | 0.9626 | 4.52E-121 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene058150 | MS.gene47121 | PPI |
| MS.gene058150 | MS.gene55486 | PPI |
| MS.gene074110 | MS.gene058150 | PPI |
| MS.gene47123 | MS.gene058150 | PPI |
| MS.gene058150 | MS.gene074111 | PPI |
| MS.gene55480 | MS.gene058150 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058150.t1 | MTR_5g008460 | 99.115 | 113 | 1 | 0 | 1 | 113 | 1 | 113 | 7.94e-82 | 234 |
| MS.gene058150.t1 | MTR_7g080803 | 89.381 | 113 | 12 | 0 | 1 | 113 | 1 | 113 | 8.10e-75 | 217 |
| MS.gene058150.t1 | MTR_7g080793 | 70.370 | 108 | 31 | 1 | 1 | 108 | 1 | 107 | 4.43e-52 | 159 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058150.t1 | AT4G10040 | 91.071 | 112 | 10 | 0 | 1 | 112 | 1 | 112 | 1.79e-75 | 219 |
| MS.gene058150.t1 | AT1G22840 | 88.393 | 112 | 13 | 0 | 1 | 112 | 1 | 112 | 8.81e-73 | 212 |
| MS.gene058150.t1 | AT1G22840 | 84.524 | 84 | 13 | 0 | 1 | 84 | 1 | 84 | 2.06e-50 | 155 |
Find 41 sgRNAs with CRISPR-Local
Find 95 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCAATCTGAATGGTTTGTT+TGG | 0.305862 | 5.4:+3392709 | MS.gene058150:CDS |
| AATAAGATCAGCACGTTCTT+GGG | 0.344346 | 5.4:-3393418 | None:intergenic |
| CAATAAGATCAGCACGTTCT+TGG | 0.348957 | 5.4:-3393419 | None:intergenic |
| ACAAGAACATGGCTGTGAAT+TGG | 0.349587 | 5.4:+3392776 | MS.gene058150:CDS |
| TTATTTGCTTAACCCCAAAA+AGG | 0.352975 | 5.4:+3392819 | MS.gene058150:CDS |
| CAAGAACATGGCTGTGAATT+GGG | 0.354427 | 5.4:+3392777 | MS.gene058150:CDS |
| AGCTGCCGAGTAGGAATATC+CGG | 0.375428 | 5.4:-3392752 | None:intergenic |
| GGTTTGTTTGGAAGGCAATC+AGG | 0.375977 | 5.4:+3392721 | MS.gene058150:CDS |
| GCTCTTGTGCAGTATATTCC+AGG | 0.394249 | 5.4:+3393363 | MS.gene058150:intron |
| GGTGTGACACTGAGCGCATT+TGG | 0.415295 | 5.4:-3391636 | None:intergenic |
| TTCTTGGGGTTTCTTTAGAC+CGG | 0.417289 | 5.4:-3393403 | None:intergenic |
| ATCTCCGGATTTGGCATCAC+CGG | 0.423980 | 5.4:-3391600 | None:intergenic |
| TCCAAACAAACCATTCAGAT+TGG | 0.424075 | 5.4:-3392710 | None:intergenic |
| ATCTGAATGGTTTGTTTGGA+AGG | 0.441498 | 5.4:+3392713 | MS.gene058150:CDS |
| TCAGTGTCACACCGTCGATA+AGG | 0.441684 | 5.4:+3391646 | MS.gene058150:CDS |
| CAGTGTCACACCGTCGATAA+GGG | 0.454494 | 5.4:+3391647 | MS.gene058150:CDS |
| GGGACCAAGATGGTATTTCC+CGG | 0.471291 | 5.4:+3393384 | MS.gene058150:CDS |
| CCTCCCGGTGATGCCAAATC+CGG | 0.475381 | 5.4:+3391596 | MS.gene058150:CDS |
| CCAAACAAACCATTCAGATT+GGG | 0.484079 | 5.4:-3392709 | None:intergenic |
| GATCTAACCTTGTTTGTGAC+CGG | 0.503624 | 5.4:-3391672 | None:intergenic |
| AGGCAATCAGGCACTACTCC+CGG | 0.508362 | 5.4:+3392733 | MS.gene058150:CDS |
| GGAAATACCATCTTGGTCCC+TGG | 0.530773 | 5.4:-3393381 | None:intergenic |
| TAGACCGGGAAATACCATCT+TGG | 0.536477 | 5.4:-3393388 | None:intergenic |
| ATCTCAGGACCCAATCTGAA+TGG | 0.539851 | 5.4:+3392700 | MS.gene058150:intron |
| TCTCCGGATTTGGCATCACC+GGG | 0.561033 | 5.4:-3391599 | None:intergenic |
| CTCTTGTGCAGTATATTCCA+GGG | 0.564315 | 5.4:+3393364 | MS.gene058150:intron |
| TCGTTTGAACAAGCACCTCC+CGG | 0.572771 | 5.4:+3391581 | MS.gene058150:CDS |
| GTTCTTGTTAGCTGCCGAGT+AGG | 0.576933 | 5.4:-3392761 | None:intergenic |
| TCTTGGGGTTTCTTTAGACC+GGG | 0.586837 | 5.4:-3393402 | None:intergenic |
| AAGGGTGCCGGTCACAAACA+AGG | 0.600344 | 5.4:+3391665 | MS.gene058150:CDS |
| CTCGGCAGCTAACAAGAACA+TGG | 0.601333 | 5.4:+3392765 | MS.gene058150:CDS |
| ATAAGATCAGCACGTTCTTG+GGG | 0.603518 | 5.4:-3393417 | None:intergenic |
| TGATCTTATTGCATATCTGA+AGG | 0.616592 | 5.4:+3393431 | MS.gene058150:CDS |
| CACACCGTCGATAAGGGTGC+CGG | 0.624319 | 5.4:+3391653 | MS.gene058150:CDS |
| TACTCCCGGATATTCCTACT+CGG | 0.628766 | 5.4:+3392747 | MS.gene058150:CDS |
| AATATATAATCACTGTGCAG+TGG | 0.632456 | 5.4:-3393457 | None:intergenic |
| GTGACCGGCACCCTTATCGA+CGG | 0.634194 | 5.4:-3391657 | None:intergenic |
| GCTGCCGAGTAGGAATATCC+GGG | 0.639197 | 5.4:-3392751 | None:intergenic |
| GTATATTCCAGGGACCAAGA+TGG | 0.661039 | 5.4:+3393374 | MS.gene058150:intron |
| GAACATGGCTGTGAATTGGG+AGG | 0.673762 | 5.4:+3392780 | MS.gene058150:CDS |
| CCGGATTTGGCATCACCGGG+AGG | 0.684893 | 5.4:-3391596 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATACTAATATAATATCAA+GGG | - | chr5.4:3392523-3392542 | None:intergenic | 10.0% |
| !!! | AATAATATTTAGTATTGATT+AGG | - | chr5.4:3393156-3393175 | None:intergenic | 10.0% |
| !! | AATCTCTATATCTATAATTT+AGG | + | chr5.4:3392582-3392601 | MS.gene058150:intron | 15.0% |
| !! | ATCATCTAATATTATTGTTT+AGG | + | chr5.4:3391996-3392015 | MS.gene058150:intron | 15.0% |
| !! | CATATACTAATATAATATCA+AGG | - | chr5.4:3392524-3392543 | None:intergenic | 15.0% |
| !! | TATTTCAATATTTGATTCAT+AGG | + | chr5.4:3392432-3392451 | MS.gene058150:intron | 15.0% |
| !!! | AAATAATTACATTTTCAATG+AGG | - | chr5.4:3391913-3391932 | None:intergenic | 15.0% |
| !! | ATAAAAAAGCTATTGTATGT+AGG | + | chr5.4:3392067-3392086 | MS.gene058150:intron | 20.0% |
| !! | ATTTGTGTTTATTTCATTGT+TGG | + | chr5.4:3391820-3391839 | MS.gene058150:intron | 20.0% |
| !! | GATTTATGATTCAAGAAATT+TGG | + | chr5.4:3391965-3391984 | MS.gene058150:intron | 20.0% |
| !! | GTGAAAATAATGTATAATCA+TGG | - | chr5.4:3391770-3391789 | None:intergenic | 20.0% |
| !! | TAAAAAAGCTATTGTATGTA+GGG | + | chr5.4:3392068-3392087 | MS.gene058150:intron | 20.0% |
| !! | TAGTACATAATATTGTGAAA+TGG | - | chr5.4:3392288-3392307 | None:intergenic | 20.0% |
| !! | TATTTGATTCATAGGTTTAT+TGG | + | chr5.4:3392440-3392459 | MS.gene058150:intron | 20.0% |
| !! | TTAGGATAATATTTGATTGA+TGG | + | chr5.4:3392014-3392033 | MS.gene058150:intron | 20.0% |
| !!! | AATATAATATCAAGGGTATA+AGG | - | chr5.4:3392516-3392535 | None:intergenic | 20.0% |
| !!! | TTGTTTTTCTTTTCGTTTAT+AGG | + | chr5.4:3393190-3393209 | MS.gene058150:intron | 20.0% |
| ! | AATAAGATTTACAGCTTAGT+TGG | + | chr5.4:3392895-3392914 | MS.gene058150:intron | 25.0% |
| ! | ATTAGCATATTACCACAATA+TGG | - | chr5.4:3393285-3393304 | None:intergenic | 25.0% |
| ! | GAATAACGTGATTATCTATT+TGG | + | chr5.4:3392653-3392672 | MS.gene058150:intron | 25.0% |
| !! | AGATTGTATTGTACCTTTTT+GGG | - | chr5.4:3392835-3392854 | None:intergenic | 25.0% |
| !! | TAGATTGTATTGTACCTTTT+TGG | - | chr5.4:3392836-3392855 | None:intergenic | 25.0% |
| !!! | TTTCTAAGATTTCCATATTG+TGG | + | chr5.4:3393220-3393239 | MS.gene058150:intron | 25.0% |
| AAACGAAAAGAAAAACAAGC+AGG | - | chr5.4:3393188-3393207 | None:intergenic | 30.0% | |
| AACGAAAAGAAAAACAAGCA+GGG | - | chr5.4:3393187-3393206 | None:intergenic | 30.0% | |
| CATAGATCAGTAACAAATTG+TGG | - | chr5.4:3393340-3393359 | None:intergenic | 30.0% | |
| TGATCTTATTGCATATCTGA+AGG | + | chr5.4:3393431-3393450 | MS.gene058150:CDS | 30.0% | |
| TGGTAATATGCTACGTTTAT+AGG | + | chr5.4:3393240-3393259 | MS.gene058150:intron | 30.0% | |
| TTAGATCAGTTTCTCATATG+TGG | + | chr5.4:3391688-3391707 | MS.gene058150:intron | 30.0% | |
| TTATTTGCTTAACCCCAAAA+AGG | + | chr5.4:3392819-3392838 | MS.gene058150:CDS | 30.0% | |
| ! | AAAGATTTTATCTCCGGATT+TGG | - | chr5.4:3391612-3391631 | None:intergenic | 30.0% |
| ! | GATTGTATTGTACCTTTTTG+GGG | - | chr5.4:3392834-3392853 | None:intergenic | 30.0% |
| !! | TAGGTTTATTGGTGTTGTAA+TGG | + | chr5.4:3392451-3392470 | MS.gene058150:intron | 30.0% |
| AACACAAATTGCATAGCATG+TGG | - | chr5.4:3391809-3391828 | None:intergenic | 35.0% | |
| AATAAGATCAGCACGTTCTT+GGG | - | chr5.4:3393421-3393440 | None:intergenic | 35.0% | |
| AATTGCATAGCATGTGGAAA+TGG | - | chr5.4:3391803-3391822 | None:intergenic | 35.0% | |
| ACCTTCACATATAACAAGCA+AGG | + | chr5.4:3393042-3393061 | MS.gene058150:intron | 35.0% | |
| AGATCAGTAACAAATTGTGG+TGG | - | chr5.4:3393337-3393356 | None:intergenic | 35.0% | |
| AGTTGTAAGCTAATCCAAAC+TGG | + | chr5.4:3393116-3393135 | MS.gene058150:intron | 35.0% | |
| ATGTGGAAATGGAACGATTA+AGG | - | chr5.4:3391792-3391811 | None:intergenic | 35.0% | |
| CCAAACAAACCATTCAGATT+GGG | - | chr5.4:3392712-3392731 | None:intergenic | 35.0% | |
| CGTAGCATATTACCACAATA+TGG | - | chr5.4:3393235-3393254 | None:intergenic | 35.0% | |
| TCCAAACAAACCATTCAGAT+TGG | - | chr5.4:3392713-3392732 | None:intergenic | 35.0% | |
| TTGATTAGGAGTGAAGCATA+TGG | - | chr5.4:3393142-3393161 | None:intergenic | 35.0% | |
| ! | GGTTCGAAAGATTTTATCTC+CGG | - | chr5.4:3391618-3391637 | None:intergenic | 35.0% |
| ! | GTTTTTCATTACTCCTTTGC+TGG | + | chr5.4:3392543-3392562 | MS.gene058150:intron | 35.0% |
| !! | AGCACAATAAGTTTGTTGCA+CGG | - | chr5.4:3391852-3391871 | None:intergenic | 35.0% |
| !! | ATCTGAATGGTTTGTTTGGA+AGG | + | chr5.4:3392713-3392732 | MS.gene058150:CDS | 35.0% |
| !! | TTGCTTGTTATATGTGAAGG+TGG | - | chr5.4:3393043-3393062 | None:intergenic | 35.0% |
| !! | TTTGCCTTGTTGATAATCTC+AGG | + | chr5.4:3392685-3392704 | MS.gene058150:intron | 35.0% |
| !!! | TGTTTTGACATAGTGCTGTA+GGG | + | chr5.4:3392339-3392358 | MS.gene058150:intron | 35.0% |
| ACAAGAACATGGCTGTGAAT+TGG | + | chr5.4:3392776-3392795 | MS.gene058150:CDS | 40.0% | |
| AGCAGTAGTATTGTCAATCG+TGG | + | chr5.4:3392199-3392218 | MS.gene058150:intron | 40.0% | |
| ATAAGATCAGCACGTTCTTG+GGG | - | chr5.4:3393420-3393439 | None:intergenic | 40.0% | |
| CAAGAACATGGCTGTGAATT+GGG | + | chr5.4:3392777-3392796 | MS.gene058150:CDS | 40.0% | |
| CAATAAGATCAGCACGTTCT+TGG | - | chr5.4:3393422-3393441 | None:intergenic | 40.0% | |
| CTCTTGTGCAGTATATTCCA+GGG | + | chr5.4:3393364-3393383 | MS.gene058150:intron | 40.0% | |
| GAAAAGAAAAACAAGCAGGG+TGG | - | chr5.4:3393184-3393203 | None:intergenic | 40.0% | |
| GCAGTAGTATTGTCAATCGT+GGG | + | chr5.4:3392200-3392219 | MS.gene058150:intron | 40.0% | |
| GTTATATGTGAAGGTGGCAA+TGG | - | chr5.4:3393037-3393056 | None:intergenic | 40.0% | |
| TTCTTGGGGTTTCTTTAGAC+CGG | - | chr5.4:3393406-3393425 | None:intergenic | 40.0% | |
| ! | GATCTAACCTTGTTTGTGAC+CGG | - | chr5.4:3391675-3391694 | None:intergenic | 40.0% |
| !! | CCCAATCTGAATGGTTTGTT+TGG | + | chr5.4:3392709-3392728 | MS.gene058150:CDS | 40.0% |
| !! | GCCTTGCTTGTTATATGTGA+AGG | - | chr5.4:3393046-3393065 | None:intergenic | 40.0% |
| !!! | CTGTTTTGACATAGTGCTGT+AGG | + | chr5.4:3392338-3392357 | MS.gene058150:intron | 40.0% |
| AGTGAAGCATATGGCCAGTT+TGG | - | chr5.4:3393133-3393152 | None:intergenic | 45.0% | |
| ATCAAACTCAGTGCCAGCAA+AGG | - | chr5.4:3392559-3392578 | None:intergenic | 45.0% | |
| ATCTCAGGACCCAATCTGAA+TGG | + | chr5.4:3392700-3392719 | MS.gene058150:intron | 45.0% | |
| GCTCTTGTGCAGTATATTCC+AGG | + | chr5.4:3393363-3393382 | MS.gene058150:intron | 45.0% | |
| GGGTCCTGAGATTATCAACA+AGG | - | chr5.4:3392692-3392711 | None:intergenic | 45.0% | |
| GTATATTCCAGGGACCAAGA+TGG | + | chr5.4:3393374-3393393 | MS.gene058150:intron | 45.0% | |
| TACTCCCGGATATTCCTACT+CGG | + | chr5.4:3392747-3392766 | MS.gene058150:CDS | 45.0% | |
| TAGACCGGGAAATACCATCT+TGG | - | chr5.4:3393391-3393410 | None:intergenic | 45.0% | |
| TCTTGGGGTTTCTTTAGACC+GGG | - | chr5.4:3393405-3393424 | None:intergenic | 45.0% | |
| ! | GGTTTGTTTGGAAGGCAATC+AGG | + | chr5.4:3392721-3392740 | MS.gene058150:CDS | 45.0% |
| ! | GTATTGTCAATCGTGGGTTG+CGG | + | chr5.4:3392206-3392225 | MS.gene058150:intron | 45.0% |
| AGCTGCCGAGTAGGAATATC+CGG | - | chr5.4:3392755-3392774 | None:intergenic | 50.0% | |
| ATCTCCGGATTTGGCATCAC+CGG | - | chr5.4:3391603-3391622 | None:intergenic | 50.0% | |
| CAGTGTCACACCGTCGATAA+GGG | + | chr5.4:3391647-3391666 | MS.gene058150:CDS | 50.0% | |
| CTCGGCAGCTAACAAGAACA+TGG | + | chr5.4:3392765-3392784 | MS.gene058150:CDS | 50.0% | |
| GAACATGGCTGTGAATTGGG+AGG | + | chr5.4:3392780-3392799 | MS.gene058150:CDS | 50.0% | |
| GGAAATACCATCTTGGTCCC+TGG | - | chr5.4:3393384-3393403 | None:intergenic | 50.0% | |
| GGGACCAAGATGGTATTTCC+CGG | + | chr5.4:3393384-3393403 | MS.gene058150:CDS | 50.0% | |
| GTTCTTGTTAGCTGCCGAGT+AGG | - | chr5.4:3392764-3392783 | None:intergenic | 50.0% | |
| TCAGTGTCACACCGTCGATA+AGG | + | chr5.4:3391646-3391665 | MS.gene058150:CDS | 50.0% | |
| TCGTTTGAACAAGCACCTCC+CGG | + | chr5.4:3391581-3391600 | MS.gene058150:CDS | 50.0% | |
| AGGCAATCAGGCACTACTCC+CGG | + | chr5.4:3392733-3392752 | MS.gene058150:CDS | 55.0% | |
| GCTGCCGAGTAGGAATATCC+GGG | - | chr5.4:3392754-3392773 | None:intergenic | 55.0% | |
| GGTGTGACACTGAGCGCATT+TGG | - | chr5.4:3391639-3391658 | None:intergenic | 55.0% | |
| TCTCCGGATTTGGCATCACC+GGG | - | chr5.4:3391602-3391621 | None:intergenic | 55.0% | |
| !! | AAGGGTGCCGGTCACAAACA+AGG | + | chr5.4:3391665-3391684 | MS.gene058150:CDS | 55.0% |
| CCTCCCGGTGATGCCAAATC+CGG | + | chr5.4:3391596-3391615 | MS.gene058150:CDS | 60.0% | |
| GTGACCGGCACCCTTATCGA+CGG | - | chr5.4:3391660-3391679 | None:intergenic | 60.0% | |
| ! | CACACCGTCGATAAGGGTGC+CGG | + | chr5.4:3391653-3391672 | MS.gene058150:CDS | 60.0% |
| CCGGATTTGGCATCACCGGG+AGG | - | chr5.4:3391599-3391618 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 3391575 | 3393470 | 3391575 | ID=MS.gene058150 |
| chr5.4 | mRNA | 3391575 | 3393470 | 3391575 | ID=MS.gene058150.t1;Parent=MS.gene058150 |
| chr5.4 | exon | 3391575 | 3391686 | 3391575 | ID=MS.gene058150.t1.exon1;Parent=MS.gene058150.t1 |
| chr5.4 | CDS | 3391575 | 3391686 | 3391575 | ID=cds.MS.gene058150.t1;Parent=MS.gene058150.t1 |
| chr5.4 | exon | 3392707 | 3392840 | 3392707 | ID=MS.gene058150.t1.exon2;Parent=MS.gene058150.t1 |
| chr5.4 | CDS | 3392707 | 3392840 | 3392707 | ID=cds.MS.gene058150.t1;Parent=MS.gene058150.t1 |
| chr5.4 | exon | 3393375 | 3393470 | 3393375 | ID=MS.gene058150.t1.exon3;Parent=MS.gene058150.t1 |
| chr5.4 | CDS | 3393375 | 3393470 | 3393375 | ID=cds.MS.gene058150.t1;Parent=MS.gene058150.t1 |
| Gene Sequence |
| Protein sequence |
