Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21918.t1 | XP_003610916.1 | 99.1 | 113 | 1 | 0 | 1 | 113 | 1 | 113 | 7.90E-59 | 236.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21918.t1 | Q9T0G2 | 91.1 | 112 | 10 | 0 | 1 | 112 | 1 | 112 | 3.7e-57 | 221.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21918.t1 | G7K7W9 | 99.1 | 113 | 1 | 0 | 1 | 113 | 1 | 113 | 5.7e-59 | 236.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058150 | MS.gene21918 | 0.9626 | 4.52E-121 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene21918 | MS.gene55486 | PPI |
| MS.gene21918 | MS.gene47121 | PPI |
| MS.gene074110 | MS.gene21918 | PPI |
| MS.gene21918 | MS.gene55480 | PPI |
| MS.gene47123 | MS.gene21918 | PPI |
| MS.gene21918 | MS.gene074111 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21918.t1 | MTR_5g008460 | 99.115 | 113 | 1 | 0 | 1 | 113 | 1 | 113 | 7.94e-82 | 234 |
| MS.gene21918.t1 | MTR_7g080803 | 89.381 | 113 | 12 | 0 | 1 | 113 | 1 | 113 | 8.10e-75 | 217 |
| MS.gene21918.t1 | MTR_7g080793 | 70.370 | 108 | 31 | 1 | 1 | 108 | 1 | 107 | 4.43e-52 | 159 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21918.t1 | AT4G10040 | 91.071 | 112 | 10 | 0 | 1 | 112 | 1 | 112 | 1.79e-75 | 219 |
| MS.gene21918.t1 | AT1G22840 | 88.393 | 112 | 13 | 0 | 1 | 112 | 1 | 112 | 8.81e-73 | 212 |
| MS.gene21918.t1 | AT1G22840 | 84.524 | 84 | 13 | 0 | 1 | 84 | 1 | 84 | 2.06e-50 | 155 |
Find 41 sgRNAs with CRISPR-Local
Find 97 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCAATCTGAATGGTTTGTT+TGG | 0.305862 | 5.1:+2463925 | MS.gene21918:CDS |
| AATAAGATCAGCACGTTCTT+GGG | 0.344346 | 5.1:-2464634 | None:intergenic |
| CAATAAGATCAGCACGTTCT+TGG | 0.348957 | 5.1:-2464635 | None:intergenic |
| ACAAGAACATGGCTGTGAAT+TGG | 0.349587 | 5.1:+2463992 | MS.gene21918:CDS |
| TTATTTGCTTAACCCCAAAA+AGG | 0.352975 | 5.1:+2464035 | MS.gene21918:CDS |
| CAAGAACATGGCTGTGAATT+GGG | 0.354427 | 5.1:+2463993 | MS.gene21918:CDS |
| AGCTGCCGAGTAGGAATATC+CGG | 0.375428 | 5.1:-2463968 | None:intergenic |
| GGTTTGTTTGGAAGGCAATC+AGG | 0.375977 | 5.1:+2463937 | MS.gene21918:CDS |
| GCTCTTGTGCAGTATATTCC+AGG | 0.394249 | 5.1:+2464579 | MS.gene21918:intron |
| GGTGTGACACTGAGCGCATT+TGG | 0.415295 | 5.1:-2462852 | None:intergenic |
| TTCTTGGGGTTTCTTTAGAC+CGG | 0.417289 | 5.1:-2464619 | None:intergenic |
| ATCTCCGGATTTGGCATCAC+CGG | 0.423980 | 5.1:-2462816 | None:intergenic |
| TCCAAACAAACCATTCAGAT+TGG | 0.424075 | 5.1:-2463926 | None:intergenic |
| ATCTGAATGGTTTGTTTGGA+AGG | 0.441498 | 5.1:+2463929 | MS.gene21918:CDS |
| TCAGTGTCACACCGTCGATA+AGG | 0.441684 | 5.1:+2462862 | MS.gene21918:CDS |
| CAGTGTCACACCGTCGATAA+GGG | 0.454494 | 5.1:+2462863 | MS.gene21918:CDS |
| GGGACCAAGATGGTATTTCC+CGG | 0.471291 | 5.1:+2464600 | MS.gene21918:CDS |
| CCTCCCGGTGATGCCAAATC+CGG | 0.475381 | 5.1:+2462812 | MS.gene21918:CDS |
| CCAAACAAACCATTCAGATT+GGG | 0.484079 | 5.1:-2463925 | None:intergenic |
| GATCTAACCTTGTTTGTGAC+CGG | 0.503624 | 5.1:-2462888 | None:intergenic |
| AGGCAATCAGGCACTACTCC+CGG | 0.508362 | 5.1:+2463949 | MS.gene21918:CDS |
| GGAAATACCATCTTGGTCCC+TGG | 0.530773 | 5.1:-2464597 | None:intergenic |
| TAGACCGGGAAATACCATCT+TGG | 0.536477 | 5.1:-2464604 | None:intergenic |
| ATCTCAGGACCCAATCTGAA+TGG | 0.539851 | 5.1:+2463916 | MS.gene21918:intron |
| TCTCCGGATTTGGCATCACC+GGG | 0.561033 | 5.1:-2462815 | None:intergenic |
| CTCTTGTGCAGTATATTCCA+GGG | 0.564315 | 5.1:+2464580 | MS.gene21918:intron |
| TCGTTTGAACAAGCACCTCC+CGG | 0.572771 | 5.1:+2462797 | MS.gene21918:CDS |
| GTTCTTGTTAGCTGCCGAGT+AGG | 0.576933 | 5.1:-2463977 | None:intergenic |
| TCTTGGGGTTTCTTTAGACC+GGG | 0.586837 | 5.1:-2464618 | None:intergenic |
| AAGGGTGCCGGTCACAAACA+AGG | 0.600344 | 5.1:+2462881 | MS.gene21918:CDS |
| CTCGGCAGCTAACAAGAACA+TGG | 0.601333 | 5.1:+2463981 | MS.gene21918:CDS |
| ATAAGATCAGCACGTTCTTG+GGG | 0.603518 | 5.1:-2464633 | None:intergenic |
| TGATCTTATTGCATATCTGA+AGG | 0.616592 | 5.1:+2464647 | MS.gene21918:CDS |
| CACACCGTCGATAAGGGTGC+CGG | 0.624319 | 5.1:+2462869 | MS.gene21918:CDS |
| TACTCCCGGATATTCCTACT+CGG | 0.628766 | 5.1:+2463963 | MS.gene21918:CDS |
| AATATATAATCACTGTGCAG+TGG | 0.632456 | 5.1:-2464673 | None:intergenic |
| GTGACCGGCACCCTTATCGA+CGG | 0.634194 | 5.1:-2462873 | None:intergenic |
| GCTGCCGAGTAGGAATATCC+GGG | 0.639197 | 5.1:-2463967 | None:intergenic |
| GTATATTCCAGGGACCAAGA+TGG | 0.661039 | 5.1:+2464590 | MS.gene21918:intron |
| GAACATGGCTGTGAATTGGG+AGG | 0.673762 | 5.1:+2463996 | MS.gene21918:CDS |
| CCGGATTTGGCATCACCGGG+AGG | 0.684893 | 5.1:-2462812 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATACTAATATAATATCAA+GGG | - | chr5.1:2463739-2463758 | None:intergenic | 10.0% |
| !!! | AATAATATTTAGTATTGATT+AGG | - | chr5.1:2464372-2464391 | None:intergenic | 10.0% |
| !! | AATCTCTATATCTATAATTT+AGG | + | chr5.1:2463798-2463817 | MS.gene21918:intron | 15.0% |
| !! | ATCATCTAATATTATTGTTT+AGG | + | chr5.1:2463212-2463231 | MS.gene21918:intron | 15.0% |
| !! | CATATACTAATATAATATCA+AGG | - | chr5.1:2463740-2463759 | None:intergenic | 15.0% |
| !! | TATTTCAATATTTGATTCAT+AGG | + | chr5.1:2463648-2463667 | MS.gene21918:intron | 15.0% |
| !!! | AAATAATTACATTTTCAATG+AGG | - | chr5.1:2463129-2463148 | None:intergenic | 15.0% |
| !! | ATAAAAAAGCTATTGTATGT+AGG | + | chr5.1:2463283-2463302 | MS.gene21918:intron | 20.0% |
| !! | ATTTGTGTTTATTTCATTGT+TGG | + | chr5.1:2463036-2463055 | MS.gene21918:intron | 20.0% |
| !! | GATTTATGATTCAAGAAATT+TGG | + | chr5.1:2463181-2463200 | MS.gene21918:intron | 20.0% |
| !! | GTGAAAATAATGTATAATCA+TGG | - | chr5.1:2462986-2463005 | None:intergenic | 20.0% |
| !! | TAAAAAAGCTATTGTATGTA+GGG | + | chr5.1:2463284-2463303 | MS.gene21918:intron | 20.0% |
| !! | TAGTACATAATATTGTGAAA+TGG | - | chr5.1:2463504-2463523 | None:intergenic | 20.0% |
| !! | TATTTGATTCATAGGTTTAT+TGG | + | chr5.1:2463656-2463675 | MS.gene21918:intron | 20.0% |
| !! | TTAGGATAATATTTGATTGA+TGG | + | chr5.1:2463230-2463249 | MS.gene21918:intron | 20.0% |
| !!! | AATATAATATCAAGGGTATA+AGG | - | chr5.1:2463732-2463751 | None:intergenic | 20.0% |
| !!! | TTGTTTTTCTTTTCGTTTAT+AGG | + | chr5.1:2464406-2464425 | MS.gene21918:intron | 20.0% |
| ! | AATAAGATTTACAGCTTAGT+TGG | + | chr5.1:2464111-2464130 | MS.gene21918:intron | 25.0% |
| ! | ATTAGCATATTACCACAATA+TGG | - | chr5.1:2464501-2464520 | None:intergenic | 25.0% |
| ! | GAATAACGTGATTATCTATT+TGG | + | chr5.1:2463869-2463888 | MS.gene21918:intron | 25.0% |
| !! | AGATTGTATTGTACCTTTTT+GGG | - | chr5.1:2464051-2464070 | None:intergenic | 25.0% |
| !! | TAGATTGTATTGTACCTTTT+TGG | - | chr5.1:2464052-2464071 | None:intergenic | 25.0% |
| !!! | TTTCTAAGATTTCCATATTG+TGG | + | chr5.1:2464436-2464455 | MS.gene21918:intron | 25.0% |
| AAACGAAAAGAAAAACAAGC+AGG | - | chr5.1:2464404-2464423 | None:intergenic | 30.0% | |
| AACGAAAAGAAAAACAAGCA+GGG | - | chr5.1:2464403-2464422 | None:intergenic | 30.0% | |
| CATAGATCAGTAACAAATTG+TGG | - | chr5.1:2464556-2464575 | None:intergenic | 30.0% | |
| TGATCTTATTGCATATCTGA+AGG | + | chr5.1:2464647-2464666 | MS.gene21918:CDS | 30.0% | |
| TGGTAATATGCTACGTTTAT+AGG | + | chr5.1:2464456-2464475 | MS.gene21918:intron | 30.0% | |
| TTAGATCAGTTTCTCATATG+TGG | + | chr5.1:2462904-2462923 | MS.gene21918:intron | 30.0% | |
| TTATTTGCTTAACCCCAAAA+AGG | + | chr5.1:2464035-2464054 | MS.gene21918:CDS | 30.0% | |
| ! | AAAGATTTTATCTCCGGATT+TGG | - | chr5.1:2462828-2462847 | None:intergenic | 30.0% |
| ! | GATTGTATTGTACCTTTTTG+GGG | - | chr5.1:2464050-2464069 | None:intergenic | 30.0% |
| !! | TAGGTTTATTGGTGTTGTAA+TGG | + | chr5.1:2463667-2463686 | MS.gene21918:intron | 30.0% |
| AACACAAATTGCATAGCATG+TGG | - | chr5.1:2463025-2463044 | None:intergenic | 35.0% | |
| AATAAGATCAGCACGTTCTT+GGG | - | chr5.1:2464637-2464656 | None:intergenic | 35.0% | |
| AATTGCATAGCATGTGGAAA+TGG | - | chr5.1:2463019-2463038 | None:intergenic | 35.0% | |
| ACCTTCACATATAACAAGCA+AGG | + | chr5.1:2464258-2464277 | MS.gene21918:intron | 35.0% | |
| AGATCAGTAACAAATTGTGG+TGG | - | chr5.1:2464553-2464572 | None:intergenic | 35.0% | |
| AGTTGTAAGCTAATCCAAAC+TGG | + | chr5.1:2464332-2464351 | MS.gene21918:intron | 35.0% | |
| ATGTGGAAATGGAACGATTA+AGG | - | chr5.1:2463008-2463027 | None:intergenic | 35.0% | |
| CCAAACAAACCATTCAGATT+GGG | - | chr5.1:2463928-2463947 | None:intergenic | 35.0% | |
| CGTAGCATATTACCACAATA+TGG | - | chr5.1:2464451-2464470 | None:intergenic | 35.0% | |
| TCCAAACAAACCATTCAGAT+TGG | - | chr5.1:2463929-2463948 | None:intergenic | 35.0% | |
| TTATATGTGAAGGTGGCAAT+GGG | - | chr5.1:2464252-2464271 | None:intergenic | 35.0% | |
| TTGATTAGGAGTGAAGCATA+TGG | - | chr5.1:2464358-2464377 | None:intergenic | 35.0% | |
| ! | GGTTCGAAAGATTTTATCTC+CGG | - | chr5.1:2462834-2462853 | None:intergenic | 35.0% |
| ! | GTTTTTCATTACTCCTTTGC+TGG | + | chr5.1:2463759-2463778 | MS.gene21918:intron | 35.0% |
| !! | AGCACAATAAGTTTGTTGCA+CGG | - | chr5.1:2463068-2463087 | None:intergenic | 35.0% |
| !! | ATCTGAATGGTTTGTTTGGA+AGG | + | chr5.1:2463929-2463948 | MS.gene21918:CDS | 35.0% |
| !! | TTGCTTGTTATATGTGAAGG+TGG | - | chr5.1:2464259-2464278 | None:intergenic | 35.0% |
| !! | TTTGCCTTGTTGATAATCTC+AGG | + | chr5.1:2463901-2463920 | MS.gene21918:intron | 35.0% |
| !!! | TGTTTTGACATAGTGCTGTA+GGG | + | chr5.1:2463555-2463574 | MS.gene21918:intron | 35.0% |
| ACAAGAACATGGCTGTGAAT+TGG | + | chr5.1:2463992-2464011 | MS.gene21918:CDS | 40.0% | |
| ATAAGATCAGCACGTTCTTG+GGG | - | chr5.1:2464636-2464655 | None:intergenic | 40.0% | |
| CAAGAACATGGCTGTGAATT+GGG | + | chr5.1:2463993-2464012 | MS.gene21918:CDS | 40.0% | |
| CAATAAGATCAGCACGTTCT+TGG | - | chr5.1:2464638-2464657 | None:intergenic | 40.0% | |
| CTCTTGTGCAGTATATTCCA+GGG | + | chr5.1:2464580-2464599 | MS.gene21918:intron | 40.0% | |
| GAAAAGAAAAACAAGCAGGG+TGG | - | chr5.1:2464400-2464419 | None:intergenic | 40.0% | |
| GTTATATGTGAAGGTGGCAA+TGG | - | chr5.1:2464253-2464272 | None:intergenic | 40.0% | |
| TATATGTGAAGGTGGCAATG+GGG | - | chr5.1:2464251-2464270 | None:intergenic | 40.0% | |
| TTCTTGGGGTTTCTTTAGAC+CGG | - | chr5.1:2464622-2464641 | None:intergenic | 40.0% | |
| ! | GATCTAACCTTGTTTGTGAC+CGG | - | chr5.1:2462891-2462910 | None:intergenic | 40.0% |
| !! | CCCAATCTGAATGGTTTGTT+TGG | + | chr5.1:2463925-2463944 | MS.gene21918:CDS | 40.0% |
| !! | GCCTTGCTTGTTATATGTGA+AGG | - | chr5.1:2464262-2464281 | None:intergenic | 40.0% |
| !!! | CTGTTTTGACATAGTGCTGT+AGG | + | chr5.1:2463554-2463573 | MS.gene21918:intron | 40.0% |
| AGCAGCAGTATTGTCAATCG+TGG | + | chr5.1:2463415-2463434 | MS.gene21918:intron | 45.0% | |
| AGTGAAGCATATGGCCAGTT+TGG | - | chr5.1:2464349-2464368 | None:intergenic | 45.0% | |
| ATCAAACTCAGTGCCAGCAA+AGG | - | chr5.1:2463775-2463794 | None:intergenic | 45.0% | |
| ATCTCAGGACCCAATCTGAA+TGG | + | chr5.1:2463916-2463935 | MS.gene21918:intron | 45.0% | |
| GCAGCAGTATTGTCAATCGT+GGG | + | chr5.1:2463416-2463435 | MS.gene21918:intron | 45.0% | |
| GCTCTTGTGCAGTATATTCC+AGG | + | chr5.1:2464579-2464598 | MS.gene21918:intron | 45.0% | |
| GGGTCCTGAGATTATCAACA+AGG | - | chr5.1:2463908-2463927 | None:intergenic | 45.0% | |
| GTATATTCCAGGGACCAAGA+TGG | + | chr5.1:2464590-2464609 | MS.gene21918:intron | 45.0% | |
| TACTCCCGGATATTCCTACT+CGG | + | chr5.1:2463963-2463982 | MS.gene21918:CDS | 45.0% | |
| TAGACCGGGAAATACCATCT+TGG | - | chr5.1:2464607-2464626 | None:intergenic | 45.0% | |
| TCTTGGGGTTTCTTTAGACC+GGG | - | chr5.1:2464621-2464640 | None:intergenic | 45.0% | |
| ! | GGTTTGTTTGGAAGGCAATC+AGG | + | chr5.1:2463937-2463956 | MS.gene21918:CDS | 45.0% |
| ! | GTATTGTCAATCGTGGGTTG+CGG | + | chr5.1:2463422-2463441 | MS.gene21918:intron | 45.0% |
| AGCTGCCGAGTAGGAATATC+CGG | - | chr5.1:2463971-2463990 | None:intergenic | 50.0% | |
| ATCTCCGGATTTGGCATCAC+CGG | - | chr5.1:2462819-2462838 | None:intergenic | 50.0% | |
| CAGTGTCACACCGTCGATAA+GGG | + | chr5.1:2462863-2462882 | MS.gene21918:CDS | 50.0% | |
| CTCGGCAGCTAACAAGAACA+TGG | + | chr5.1:2463981-2464000 | MS.gene21918:CDS | 50.0% | |
| GAACATGGCTGTGAATTGGG+AGG | + | chr5.1:2463996-2464015 | MS.gene21918:CDS | 50.0% | |
| GGAAATACCATCTTGGTCCC+TGG | - | chr5.1:2464600-2464619 | None:intergenic | 50.0% | |
| GGGACCAAGATGGTATTTCC+CGG | + | chr5.1:2464600-2464619 | MS.gene21918:CDS | 50.0% | |
| GTTCTTGTTAGCTGCCGAGT+AGG | - | chr5.1:2463980-2463999 | None:intergenic | 50.0% | |
| TCAGTGTCACACCGTCGATA+AGG | + | chr5.1:2462862-2462881 | MS.gene21918:CDS | 50.0% | |
| TCGTTTGAACAAGCACCTCC+CGG | + | chr5.1:2462797-2462816 | MS.gene21918:CDS | 50.0% | |
| AGGCAATCAGGCACTACTCC+CGG | + | chr5.1:2463949-2463968 | MS.gene21918:CDS | 55.0% | |
| GCTGCCGAGTAGGAATATCC+GGG | - | chr5.1:2463970-2463989 | None:intergenic | 55.0% | |
| GGTGTGACACTGAGCGCATT+TGG | - | chr5.1:2462855-2462874 | None:intergenic | 55.0% | |
| TCTCCGGATTTGGCATCACC+GGG | - | chr5.1:2462818-2462837 | None:intergenic | 55.0% | |
| !! | AAGGGTGCCGGTCACAAACA+AGG | + | chr5.1:2462881-2462900 | MS.gene21918:CDS | 55.0% |
| CCTCCCGGTGATGCCAAATC+CGG | + | chr5.1:2462812-2462831 | MS.gene21918:CDS | 60.0% | |
| GTGACCGGCACCCTTATCGA+CGG | - | chr5.1:2462876-2462895 | None:intergenic | 60.0% | |
| ! | CACACCGTCGATAAGGGTGC+CGG | + | chr5.1:2462869-2462888 | MS.gene21918:CDS | 60.0% |
| CCGGATTTGGCATCACCGGG+AGG | - | chr5.1:2462815-2462834 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 2462791 | 2464686 | 2462791 | ID=MS.gene21918 |
| chr5.1 | mRNA | 2462791 | 2464686 | 2462791 | ID=MS.gene21918.t1;Parent=MS.gene21918 |
| chr5.1 | exon | 2462791 | 2462902 | 2462791 | ID=MS.gene21918.t1.exon1;Parent=MS.gene21918.t1 |
| chr5.1 | CDS | 2462791 | 2462902 | 2462791 | ID=cds.MS.gene21918.t1;Parent=MS.gene21918.t1 |
| chr5.1 | exon | 2463923 | 2464056 | 2463923 | ID=MS.gene21918.t1.exon2;Parent=MS.gene21918.t1 |
| chr5.1 | CDS | 2463923 | 2464056 | 2463923 | ID=cds.MS.gene21918.t1;Parent=MS.gene21918.t1 |
| chr5.1 | exon | 2464591 | 2464686 | 2464591 | ID=MS.gene21918.t1.exon3;Parent=MS.gene21918.t1 |
| chr5.1 | CDS | 2464591 | 2464686 | 2464591 | ID=cds.MS.gene21918.t1;Parent=MS.gene21918.t1 |
| Gene Sequence |
| Protein sequence |
