AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene22157


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22157.t1 MTR_3g047140 76.344 279 60 3 1 274 1 278 4.60e-145 408
MS.gene22157.t1 MTR_0163s0020 64.621 277 92 2 1 273 2 276 1.34e-110 321
MS.gene22157.t1 MTR_0684s0020 64.621 277 92 2 1 273 1 275 1.70e-110 320
MS.gene22157.t1 MTR_3g054170 54.545 242 105 3 17 257 17 254 3.11e-81 245
MS.gene22157.t1 MTR_3g054180 54.098 244 105 4 17 257 21 260 4.64e-79 240
MS.gene22157.t1 MTR_1g090973 49.810 263 123 5 1 257 1 260 4.27e-78 238
MS.gene22157.t1 MTR_3g054200 50.602 249 118 3 10 257 10 254 2.35e-73 225
MS.gene22157.t1 MTR_1g090943 48.679 265 127 7 10 271 15 273 3.28e-69 215
MS.gene22157.t1 MTR_1g090960 45.000 280 104 8 1 269 1 241 1.53e-63 199
MS.gene22157.t1 MTR_3g027280 48.239 284 123 14 1 267 1 277 5.15e-61 194
MS.gene22157.t1 MTR_1g090937 42.908 282 134 13 1 267 1 270 1.03e-50 167
MS.gene22157.t1 MTR_1g090957 45.000 280 120 15 1 264 1 262 5.53e-50 166
MS.gene22157.t1 MTR_1g090930 41.319 288 138 11 1 269 1 276 3.70e-48 162
MS.gene22157.t1 MTR_4g023810 41.736 242 129 7 26 263 27 260 1.64e-43 149
MS.gene22157.t1 MTR_8g068040 38.007 271 146 10 1 258 1 262 3.85e-42 145
MS.gene22157.t1 MTR_8g023510 40.755 265 143 10 1 257 1 259 5.92e-42 145
MS.gene22157.t1 MTR_8g068030 37.729 273 142 11 1 255 1 263 5.92e-41 142
MS.gene22157.t1 MTR_8g068050 37.594 266 141 8 9 259 20 275 3.09e-38 142
MS.gene22157.t1 MTR_5g031160 37.200 250 135 8 26 257 42 287 6.74e-34 124
MS.gene22157.t1 MTR_8g067940 35.531 273 153 9 3 255 10 279 1.19e-33 124
MS.gene22157.t1 MTR_4g093080 34.496 258 151 8 13 258 9 260 1.20e-33 129
MS.gene22157.t1 MTR_5g031090 34.413 247 140 8 30 257 32 275 1.70e-33 123
MS.gene22157.t1 MTR_8g067980 37.083 240 128 11 32 255 25 257 8.20e-33 121
MS.gene22157.t1 MTR_5g031120 36.992 246 132 8 31 257 38 279 1.22e-32 121
MS.gene22157.t1 MTR_5g031030 34.568 243 141 8 30 257 35 274 2.26e-32 120
MS.gene22157.t1 MTR_5g031140 38.115 244 131 9 31 257 46 286 4.98e-32 119
MS.gene22157.t1 MTR_8g067990 37.759 241 125 12 32 255 25 257 5.52e-32 119
MS.gene22157.t1 MTR_4g056320 35.971 278 127 12 2 270 6 241 1.12e-31 118
MS.gene22157.t1 MTR_5g031100 35.772 246 134 9 31 257 38 278 5.27e-30 114
MS.gene22157.t1 MTR_7g115740 34.335 233 124 11 46 258 38 261 8.03e-30 118
MS.gene22157.t1 MTR_8g067735 33.333 249 151 8 31 267 43 288 8.62e-30 118
MS.gene22157.t1 MTR_4g093110 34.799 273 133 13 10 257 12 264 2.09e-28 114
MS.gene22157.t1 MTR_7g116130 33.712 264 140 13 19 260 14 264 1.46e-27 112
MS.gene22157.t1 MTR_4g093140 33.456 272 138 11 10 257 12 264 1.87e-27 111
MS.gene22157.t1 MTR_4g093040 32.794 247 144 9 11 240 13 254 2.39e-27 111
MS.gene22157.t1 MTR_8g067960 37.168 226 120 10 46 257 24 241 4.68e-26 102
MS.gene22157.t1 MTR_4g093040 39.306 173 88 7 82 240 3 172 7.59e-26 106
MS.gene22157.t1 MTR_1g014240 35.407 209 118 6 71 265 78 283 9.00e-25 103
MS.gene22157.t1 MTR_8g067630 33.040 227 131 8 31 243 30 249 1.10e-23 100
MS.gene22157.t1 MTR_8g067930 30.522 249 158 7 31 267 24 269 1.54e-23 100
MS.gene22157.t1 MTR_4g093070 34.328 268 143 9 10 257 12 266 1.90e-23 100
MS.gene22157.t1 MTR_8g067720 30.153 262 160 9 11 260 10 260 2.98e-23 99.4
MS.gene22157.t1 MTR_3g463370 31.618 272 130 9 9 269 8 234 9.66e-23 94.0
MS.gene22157.t1 MTR_8g067690 29.412 238 154 6 31 257 60 294 8.51e-21 92.0
MS.gene22157.t1 MTR_4g093050 30.980 255 155 10 16 253 12 262 7.72e-19 86.3
MS.gene22157.t1 MTR_5g025020 33.690 187 103 7 72 240 88 271 8.06e-19 86.3
MS.gene22157.t1 MTR_7g063010 30.544 239 127 11 42 254 32 257 9.59e-16 77.4
MS.gene22157.t1 MTR_1g048360 28.458 253 162 6 15 256 17 261 1.59e-15 76.6
MS.gene22157.t1 MTR_5g077100 32.273 220 129 7 50 259 45 254 2.08e-15 76.3
MS.gene22157.t1 MTR_2g090410 34.694 147 85 6 46 186 34 175 5.53e-14 72.4
MS.gene22157.t1 MTR_7g115740 32.593 135 70 7 46 167 38 164 1.07e-13 71.2
MS.gene22157.t1 MTR_7g062940 29.167 216 127 9 59 254 47 256 2.27e-13 70.1
MS.gene22157.t1 MTR_2g090250 26.872 227 148 5 44 253 34 259 4.91e-13 69.3
MS.gene22157.t1 MTR_3g068025 28.346 254 137 10 33 254 28 268 1.10e-12 68.2
MS.gene22157.t1 MTR_5g068770 27.570 214 132 7 72 267 81 289 1.47e-12 67.8
MS.gene22157.t1 MTR_8g068050 28.743 167 105 4 9 170 20 177 4.47e-11 63.2
MS.gene22157.t1 MTR_7g062890 30.244 205 116 9 72 254 58 257 4.51e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22157.t1 AT5G10530 35.185 270 142 14 6 258 2 255 1.24e-24 103
MS.gene22157.t1 AT5G65600 34.211 190 106 7 71 243 76 263 7.14e-20 89.7
MS.gene22157.t1 AT5G55830 30.258 271 160 11 6 255 4 266 1.54e-17 82.8
MS.gene22157.t1 AT5G03140 29.245 212 135 6 50 251 51 257 2.40e-15 76.3
MS.gene22157.t1 AT5G06740 28.571 259 155 13 18 256 23 271 6.96e-14 71.6
MS.gene22157.t1 AT5G06740 28.571 259 155 13 18 256 11 259 7.31e-14 71.6
MS.gene22157.t1 AT4G29050 27.363 201 127 5 72 254 60 259 2.78e-13 70.1
MS.gene22157.t1 AT4G29050 27.363 201 127 5 72 254 74 273 3.17e-13 69.7
MS.gene22157.t1 AT3G55550 29.583 240 142 9 34 253 26 258 6.17e-13 68.9
MS.gene22157.t1 AT4G02420 33.824 204 105 11 72 253 65 260 1.50e-12 67.8
MS.gene22157.t1 AT1G53080 29.258 229 129 10 59 258 57 281 5.13e-11 62.4
MS.gene22157.t1 AT4G04960 33.775 151 90 4 46 190 35 181 8.23e-11 62.4

Find 51 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGACCAATGCTAACTGTTTC+TGG 0.178047 3.4:-41885518 None:intergenic
AGATTTGGAATTACACTATT+TGG 0.215421 3.4:-41885197 None:intergenic
ATTAACCCTTATACGATTAT+AGG 0.243083 3.4:+41885032 MS.gene22157:CDS
CCATCTGTGTTGTTAAGATT+TGG 0.245140 3.4:-41885212 None:intergenic
GCACGACCTATAATCGTATA+AGG 0.280994 3.4:-41885038 None:intergenic
GATCCTAACAATGCACATAT+TGG 0.297717 3.4:+41885317 MS.gene22157:CDS
TTGATAATTATCACAATGAT+TGG 0.305021 3.4:+41885294 MS.gene22157:CDS
TCAAGGGAATGCTGTCATTC+AGG 0.316108 3.4:+41884983 MS.gene22157:CDS
ACAGTTAGCATTGGTCTATC+TGG 0.319313 3.4:+41885524 MS.gene22157:CDS
ATTCAACACCCGTTCCCATT+TGG 0.342088 3.4:+41885066 MS.gene22157:CDS
CAACACAGATGGTGGAGATC+TGG 0.350959 3.4:+41885223 MS.gene22157:CDS
GGGAACGGGTGTTGAATATA+AGG 0.357167 3.4:-41885060 None:intergenic
TTCAACACCCGTTCCCATTT+GGG 0.360630 3.4:+41885067 MS.gene22157:CDS
GAAACTCACTTGGAGCTTAC+TGG 0.370616 3.4:+41885005 MS.gene22157:CDS
CCCCATGGGATTCACAAAAC+GGG 0.386898 3.4:+41885375 MS.gene22157:CDS
TGAATATGACTAACAGAATC+TGG 0.399885 3.4:-41884860 None:intergenic
ACTGCTTTCAATCGATTTGT+TGG 0.405940 3.4:+41885263 MS.gene22157:CDS
ATCCTAACAATGCACATATT+GGG 0.411998 3.4:+41885318 MS.gene22157:CDS
CCCATTTGGGATAGTGCAAC+TGG 0.412436 3.4:+41885080 MS.gene22157:CDS
GCCAGTTGCACTATCCCAAA+TGG 0.414802 3.4:-41885081 None:intergenic
GACTTAGCTGATTACATCTC+TGG 0.418401 3.4:+41884942 MS.gene22157:CDS
ACTAACAGAATCTGGTTAGT+TGG 0.431026 3.4:-41884852 None:intergenic
GAATGACAGCATTCCCTTGA+AGG 0.432002 3.4:-41884980 None:intergenic
TCCCCATGGGATTCACAAAA+CGG 0.443828 3.4:+41885374 MS.gene22157:CDS
TGATAATTATCACAATGATT+GGG 0.445401 3.4:+41885295 MS.gene22157:CDS
ATCCCAATATGTGCATTGTT+AGG 0.450564 3.4:-41885320 None:intergenic
GACCAATGCTAACTGTTTCT+GGG 0.454186 3.4:-41885517 None:intergenic
TGAATCCCATGGGGATGTCT+TGG 0.462545 3.4:-41885366 None:intergenic
GTTTCTCTCATTGACGAGAA+TGG 0.472278 3.4:+41885449 MS.gene22157:CDS
CTCAGTTACTAGCAAGAGAA+AGG 0.473501 3.4:-41884889 None:intergenic
GCACTATCCCAAATGGGAAC+GGG 0.488710 3.4:-41885074 None:intergenic
AACACAGATGGTGGAGATCT+GGG 0.502642 3.4:+41885224 MS.gene22157:CDS
CACGACCTATAATCGTATAA+GGG 0.505615 3.4:-41885037 None:intergenic
TTTGGAATTACACTATTTGG+TGG 0.521911 3.4:-41885194 None:intergenic
CCAGTTGCACTATCCCAAAT+GGG 0.537384 3.4:-41885080 None:intergenic
TGCACTATCCCAAATGGGAA+CGG 0.540036 3.4:-41885075 None:intergenic
AATGACAGCATTCCCTTGAA+GGG 0.551329 3.4:-41884979 None:intergenic
GGTAGTTATCCACGAGCTGA+TGG 0.555952 3.4:+41885152 MS.gene22157:CDS
CTCCCAGAAACAGTTAGCAT+TGG 0.565328 3.4:+41885515 MS.gene22157:CDS
CCAAATCTTAACAACACAGA+TGG 0.577172 3.4:+41885212 MS.gene22157:CDS
AATCTTAACAACACAGATGG+TGG 0.587455 3.4:+41885215 MS.gene22157:CDS
TGTCATTCAGGAAACTCACT+TGG 0.593945 3.4:+41884995 MS.gene22157:CDS
CAAATCAACGACTTGAGCAA+CGG 0.597604 3.4:-41885483 None:intergenic
TTCATCCAAGACATCCCCAT+GGG 0.607724 3.4:+41885361 MS.gene22157:CDS
AAGATAAGACCATCAGCTCG+TGG 0.612801 3.4:-41885161 None:intergenic
GATGTAATCAGCTAAGTCGA+AGG 0.627754 3.4:-41884937 None:intergenic
AAATATATGTCATTGAAACA+TGG 0.630792 3.4:+41885570 MS.gene22157:CDS
CCCATGGGATTCACAAAACG+GGG 0.631871 3.4:+41885376 MS.gene22157:CDS
AGTATGATTTATGTTCTCTG+AGG 0.647177 3.4:-41885633 None:intergenic
TTTCATCCAAGACATCCCCA+TGG 0.656398 3.4:+41885360 MS.gene22157:CDS
GCTCGTGGATAACTACCGAA+CGG 0.706851 3.4:-41885146 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATATGTCATTGAAACA+TGG + chr3.4:41885570-41885589 MS.gene22157:CDS 20.0%
!! TGATAATTATCACAATGATT+GGG + chr3.4:41885295-41885314 MS.gene22157:CDS 20.0%
!!! TTGATAATTATCACAATGAT+TGG + chr3.4:41885294-41885313 MS.gene22157:CDS 20.0%
! AAAAAACTTTAACCCTTCAA+GGG + chr3.4:41884967-41884986 MS.gene22157:CDS 25.0%
! ATTAACCCTTATACGATTAT+AGG + chr3.4:41885032-41885051 MS.gene22157:CDS 25.0%
!! AGATTTGGAATTACACTATT+TGG - chr3.4:41885200-41885219 None:intergenic 25.0%
AGTATGATTTATGTTCTCTG+AGG - chr3.4:41885636-41885655 None:intergenic 30.0%
ATCCTAACAATGCACATATT+GGG + chr3.4:41885318-41885337 MS.gene22157:CDS 30.0%
GAAAAAACTTTAACCCTTCA+AGG + chr3.4:41884966-41884985 MS.gene22157:CDS 30.0%
TGAATATGACTAACAGAATC+TGG - chr3.4:41884863-41884882 None:intergenic 30.0%
! TTTGGAATTACACTATTTGG+TGG - chr3.4:41885197-41885216 None:intergenic 30.0%
AATCTTAACAACACAGATGG+TGG + chr3.4:41885215-41885234 MS.gene22157:CDS 35.0%
ACTAACAGAATCTGGTTAGT+TGG - chr3.4:41884855-41884874 None:intergenic 35.0%
ATCCCAATATGTGCATTGTT+AGG - chr3.4:41885323-41885342 None:intergenic 35.0%
CACGACCTATAATCGTATAA+GGG - chr3.4:41885040-41885059 None:intergenic 35.0%
CCAAATCTTAACAACACAGA+TGG + chr3.4:41885212-41885231 MS.gene22157:CDS 35.0%
GATCCTAACAATGCACATAT+TGG + chr3.4:41885317-41885336 MS.gene22157:CDS 35.0%
! CCATCTGTGTTGTTAAGATT+TGG - chr3.4:41885215-41885234 None:intergenic 35.0%
!! ACTGCTTTCAATCGATTTGT+TGG + chr3.4:41885263-41885282 MS.gene22157:CDS 35.0%
AATGACAGCATTCCCTTGAA+GGG - chr3.4:41884982-41885001 None:intergenic 40.0%
AGACCAATGCTAACTGTTTC+TGG - chr3.4:41885521-41885540 None:intergenic 40.0%
CAAATCAACGACTTGAGCAA+CGG - chr3.4:41885486-41885505 None:intergenic 40.0%
CTCAGTTACTAGCAAGAGAA+AGG - chr3.4:41884892-41884911 None:intergenic 40.0%
GACTTAGCTGATTACATCTC+TGG + chr3.4:41884942-41884961 MS.gene22157:CDS 40.0%
GATGTAATCAGCTAAGTCGA+AGG - chr3.4:41884940-41884959 None:intergenic 40.0%
GCACGACCTATAATCGTATA+AGG - chr3.4:41885041-41885060 None:intergenic 40.0%
GTTTCTCTCATTGACGAGAA+TGG + chr3.4:41885449-41885468 MS.gene22157:CDS 40.0%
TGTCATTCAGGAAACTCACT+TGG + chr3.4:41884995-41885014 MS.gene22157:CDS 40.0%
! GACCAATGCTAACTGTTTCT+GGG - chr3.4:41885520-41885539 None:intergenic 40.0%
!! ACAGTTAGCATTGGTCTATC+TGG + chr3.4:41885524-41885543 MS.gene22157:CDS 40.0%
!! TCTTTTTCTCTTGTGCCGTT+CGG + chr3.4:41885131-41885150 MS.gene22157:CDS 40.0%
!! TTGACGTTTTTCAACTGACG+TGG - chr3.4:41885552-41885571 None:intergenic 40.0%
AAGATAAGACCATCAGCTCG+TGG - chr3.4:41885164-41885183 None:intergenic 45.0%
ATTCAACACCCGTTCCCATT+TGG + chr3.4:41885066-41885085 MS.gene22157:CDS 45.0%
CCAGTTGCACTATCCCAAAT+GGG - chr3.4:41885083-41885102 None:intergenic 45.0%
CGGCACAAGAGAAAAAGAGA+AGG - chr3.4:41885129-41885148 None:intergenic 45.0%
CTCCCAGAAACAGTTAGCAT+TGG + chr3.4:41885515-41885534 MS.gene22157:CDS 45.0%
GAAACTCACTTGGAGCTTAC+TGG + chr3.4:41885005-41885024 MS.gene22157:CDS 45.0%
GAATGACAGCATTCCCTTGA+AGG - chr3.4:41884983-41885002 None:intergenic 45.0%
TCAAGGGAATGCTGTCATTC+AGG + chr3.4:41884983-41885002 MS.gene22157:CDS 45.0%
TCCCCATGGGATTCACAAAA+CGG + chr3.4:41885374-41885393 MS.gene22157:CDS 45.0%
TGCACTATCCCAAATGGGAA+CGG - chr3.4:41885078-41885097 None:intergenic 45.0%
TTCAACACCCGTTCCCATTT+GGG + chr3.4:41885067-41885086 MS.gene22157:CDS 45.0%
TTCATCCAAGACATCCCCAT+GGG + chr3.4:41885361-41885380 MS.gene22157:CDS 45.0%
TTTCATCCAAGACATCCCCA+TGG + chr3.4:41885360-41885379 MS.gene22157:CDS 45.0%
!! AACACAGATGGTGGAGATCT+GGG + chr3.4:41885224-41885243 MS.gene22157:CDS 45.0%
!! GGGAACGGGTGTTGAATATA+AGG - chr3.4:41885063-41885082 None:intergenic 45.0%
CCCATGGGATTCACAAAACG+GGG + chr3.4:41885376-41885395 MS.gene22157:CDS 50.0%
CCCCATGGGATTCACAAAAC+GGG + chr3.4:41885375-41885394 MS.gene22157:CDS 50.0%
GCACTATCCCAAATGGGAAC+GGG - chr3.4:41885077-41885096 None:intergenic 50.0%
GCCAGTTGCACTATCCCAAA+TGG - chr3.4:41885084-41885103 None:intergenic 50.0%
GCTCGTGGATAACTACCGAA+CGG - chr3.4:41885149-41885168 None:intergenic 50.0%
GGTAGTTATCCACGAGCTGA+TGG + chr3.4:41885152-41885171 MS.gene22157:CDS 50.0%
TGAATCCCATGGGGATGTCT+TGG - chr3.4:41885369-41885388 None:intergenic 50.0%
! ACCCCGTTTTGTGAATCCCA+TGG - chr3.4:41885380-41885399 None:intergenic 50.0%
! CCCATTTGGGATAGTGCAAC+TGG + chr3.4:41885080-41885099 MS.gene22157:CDS 50.0%
! CCCCGTTTTGTGAATCCCAT+GGG - chr3.4:41885379-41885398 None:intergenic 50.0%
! CCCGTTTTGTGAATCCCATG+GGG - chr3.4:41885378-41885397 None:intergenic 50.0%
!! CAACACAGATGGTGGAGATC+TGG + chr3.4:41885223-41885242 MS.gene22157:CDS 50.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 41884840 41885664 41884840 ID=MS.gene22157
chr3.4 mRNA 41884840 41885664 41884840 ID=MS.gene22157.t1;Parent=MS.gene22157
chr3.4 exon 41884840 41885664 41884840 ID=MS.gene22157.t1.exon1;Parent=MS.gene22157.t1
chr3.4 CDS 41884840 41885664 41884840 ID=cds.MS.gene22157.t1;Parent=MS.gene22157.t1
Gene Sequence

>MS.gene22157

ATGGCTTTCTTTCCAACTAACCAGATTCTGTTAGTCATATTCACAACTTCCTTTCTCTTGCTAGTAACTGAGTTTGTCAACTCAGACGAAACAGTTTCCTTCGACTTAGCTGATTACATCTCTGGTGAAAAAACTTTAACCCTTCAAGGGAATGCTGTCATTCAGGAAACTCACTTGGAGCTTACTGGTGACATTAACCCTTATACGATTATAGGTCGTGCCTTATATTCAACACCCGTTCCCATTTGGGATAGTGCAACTGGCAATGTCGCTAGTTTTGTGACTTCCTTCTCTTTTTCTCTTGTGCCGTTCGGTAGTTATCCACGAGCTGATGGTCTTATCTTCTTTCTTGCACCACCAAATAGTGTAATTCCAAATCTTAACAACACAGATGGTGGAGATCTGGGAGTAGTCGATGAAACAACTGCTTTCAATCGATTTGTTGGTGTAGAATTTGATAATTATCACAATGATTGGGATCCTAACAATGCACATATTGGGATCGATGTCAACTCTTTAATTTCATCCAAGACATCCCCATGGGATTCACAAAACGGGGTGTTGTACAATGTTAGTATCATCTATGATTCTTCTTCTAAGACTTTGAGTGTTTCTCTCATTGACGAGAATGGTCAACTTTCTACCGTTGCTCAAGTCGTTGATTTGAAAGCTGTGCTCCCAGAAACAGTTAGCATTGGTCTATCTGGTTCCACGTCAGTTGAAAAACGTCAAATATATGTCATTGAAACATGGTCTTTCAAGTCAATTTTGAAGACAACTATAAGCAGCAGCACCTCAGAGAACATAAATCATACTGCAATCTAG

Protein sequence

>MS.gene22157.t1

MAFFPTNQILLVIFTTSFLLLVTEFVNSDETVSFDLADYISGEKTLTLQGNAVIQETHLELTGDINPYTIIGRALYSTPVPIWDSATGNVASFVTSFSFSLVPFGSYPRADGLIFFLAPPNSVIPNLNNTDGGDLGVVDETTAFNRFVGVEFDNYHNDWDPNNAHIGIDVNSLISSKTSPWDSQNGVLYNVSIIYDSSSKTLSVSLIDENGQLSTVAQVVDLKAVLPETVSIGLSGSTSVEKRQIYVIETWSFKSILKTTISSSTSENINHTAI