Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22465.t1 | XP_003625582.1 | 100 | 142 | 0 | 0 | 1 | 142 | 1 | 142 | 7.10E-73 | 283.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22465.t1 | P49201 | 96.5 | 142 | 5 | 0 | 1 | 142 | 1 | 142 | 2.1e-73 | 276.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22465.t1 | B7FHD2 | 100.0 | 142 | 0 | 0 | 1 | 142 | 1 | 142 | 5.1e-73 | 283.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050290 | MS.gene22465 | 0.851837 | 6.84E-61 | -1.69E-46 |
| MS.gene050916 | MS.gene22465 | 0.811915 | 5.51E-51 | -1.69E-46 |
| MS.gene054937 | MS.gene22465 | 0.85294 | 3.32E-61 | -1.69E-46 |
| MS.gene057052 | MS.gene22465 | 0.830522 | 2.80E-55 | -1.69E-46 |
| MS.gene058560 | MS.gene22465 | 0.81253 | 4.05E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22465.t1 | MTR_7g100720 | 100.000 | 142 | 0 | 0 | 1 | 142 | 1 | 142 | 5.97e-101 | 285 |
| MS.gene22465.t1 | MTR_1g066860 | 93.662 | 142 | 9 | 0 | 1 | 142 | 1 | 142 | 1.98e-94 | 269 |
| MS.gene22465.t1 | MTR_1g066860 | 94.118 | 136 | 8 | 0 | 7 | 142 | 1 | 136 | 1.66e-90 | 259 |
| MS.gene22465.t1 | MTR_7g061155 | 99.153 | 118 | 1 | 0 | 25 | 142 | 138 | 255 | 6.91e-82 | 241 |
| MS.gene22465.t1 | MTR_7g063460 | 52.857 | 70 | 30 | 2 | 40 | 108 | 13 | 80 | 1.73e-15 | 67.4 |
| MS.gene22465.t1 | MTR_8g447270 | 64.151 | 53 | 17 | 1 | 54 | 106 | 17 | 67 | 2.67e-14 | 65.1 |
| MS.gene22465.t1 | MTR_4g038400 | 67.347 | 49 | 13 | 1 | 56 | 104 | 103 | 148 | 2.41e-12 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22465.t1 | AT5G02960 | 96.479 | 142 | 5 | 0 | 1 | 142 | 1 | 142 | 3.92e-98 | 278 |
| MS.gene22465.t1 | AT3G09680 | 92.958 | 142 | 10 | 0 | 1 | 142 | 1 | 142 | 2.03e-93 | 266 |
Find 40 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAAGGTCGTGAAGGTTTC+TGG | 0.178129 | 7.1:-8265333 | MS.gene22465:CDS |
| GAAGTCTTGATCGCTGGATT+TGG | 0.312989 | 7.1:-8265396 | MS.gene22465:CDS |
| TAATTCAAGCAACCATCATT+TGG | 0.323107 | 7.1:+8265569 | None:intergenic |
| CATGCTGTTGGTGATATTCC+TGG | 0.406074 | 7.1:-8265363 | MS.gene22465:CDS |
| AATTCAAGCAACCATCATTT+GGG | 0.428558 | 7.1:+8265570 | None:intergenic |
| CACTTACGAATGGCAGAGTT+GGG | 0.434931 | 7.1:+8265641 | None:intergenic |
| CTGCCATTCGTAAGTGTGCT+AGG | 0.435504 | 7.1:-8265634 | MS.gene22465:CDS |
| TTGGTGATATTCCTGGAGTC+AGG | 0.441270 | 7.1:-8265356 | MS.gene22465:CDS |
| AGCATGGGATGAACCAGAAA+AGG | 0.446685 | 7.1:+8265812 | None:intergenic |
| TTCTGGTTCATCCCATGCTA+AGG | 0.454550 | 7.1:-8265808 | MS.gene22465:CDS |
| AATTTAAGAAATCTCATCTT+GGG | 0.454713 | 7.1:-8265851 | MS.gene22465:CDS |
| AATCTCATCTTGGGAATGAA+TGG | 0.457554 | 7.1:-8265842 | MS.gene22465:CDS |
| CAATTTAAGAAATCTCATCT+TGG | 0.461581 | 7.1:-8265852 | MS.gene22465:CDS |
| CAGCCCACCTTTGCTTGATA+CGG | 0.462913 | 7.1:+8265880 | None:intergenic |
| TAGGATGAAGTCTTGATCGC+TGG | 0.469665 | 7.1:-8265402 | MS.gene22465:CDS |
| GCTGCCTTTGTCCCAAATGA+TGG | 0.476668 | 7.1:-8265581 | MS.gene22465:CDS |
| TGCAGGAAGACAAGAGGAAT+GGG | 0.492442 | 7.1:-8265927 | MS.gene22465:intron |
| CAAGAACAATTCCCTTAGCA+TGG | 0.494037 | 7.1:+8265796 | None:intergenic |
| ACACTTACGAATGGCAGAGT+TGG | 0.515105 | 7.1:+8265640 | None:intergenic |
| ATCGCTGGATTTGGACGTAA+AGG | 0.518707 | 7.1:-8265387 | MS.gene22465:CDS |
| GCATGGGATGAACCAGAAAA+GGG | 0.518943 | 7.1:+8265813 | None:intergenic |
| AGGAGAAGAAGGAGAAGCCT+AGG | 0.519158 | 7.1:-8265287 | MS.gene22465:CDS |
| TATTCCTGGAGTCAGGTTCA+AGG | 0.524415 | 7.1:-8265349 | MS.gene22465:CDS |
| CTGCAGGAAGACAAGAGGAA+TGG | 0.532735 | 7.1:-8265928 | MS.gene22465:intron |
| AAGAACAATTCCCTTAGCAT+GGG | 0.533822 | 7.1:+8265797 | None:intergenic |
| TCTGGTTCATCCCATGCTAA+GGG | 0.536691 | 7.1:-8265807 | MS.gene22465:CDS |
| TGCCATTCGTAAGTGTGCTA+GGG | 0.546102 | 7.1:-8265633 | MS.gene22465:CDS |
| GCAACCATCATTTGGGACAA+AGG | 0.566362 | 7.1:+8265577 | None:intergenic |
| TGGGAGCTGCTCGCAAGCTG+AGG | 0.577320 | 7.1:-8265908 | MS.gene22465:CDS |
| ACGACCTTGAACCTGACTCC+AGG | 0.578153 | 7.1:+8265345 | None:intergenic |
| GACTCCGTATCAAGCAAAGG+TGG | 0.586634 | 7.1:-8265884 | MS.gene22465:CDS |
| TCTTCTCCTTGAAAAGAGCA+AGG | 0.589001 | 7.1:+8265301 | None:intergenic |
| TTTGTCTGCAGGAAGACAAG+AGG | 0.606196 | 7.1:-8265933 | MS.gene22465:intron |
| GGACGTAAAGGTCATGCTGT+TGG | 0.616141 | 7.1:-8265375 | MS.gene22465:CDS |
| AACCCTAGCACACTTACGAA+TGG | 0.626506 | 7.1:+8265631 | None:intergenic |
| TGGTTGCTTGAATTACATTG+AGG | 0.642212 | 7.1:-8265561 | MS.gene22465:CDS |
| AGTCAGGTTCAAGGTCGTGA+AGG | 0.647492 | 7.1:-8265340 | MS.gene22465:CDS |
| ACTCCGTATCAAGCAAAGGT+GGG | 0.652737 | 7.1:-8265883 | MS.gene22465:CDS |
| GGAGACTCCGTATCAAGCAA+AGG | 0.654928 | 7.1:-8265887 | MS.gene22465:CDS |
| ATCCCACAACTTCAAGACCT+AGG | 0.665200 | 7.1:+8265270 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAAAAAATTTAGTTAATTTT+AGG | - | chr7.1:8265714-8265733 | MS.gene22465:intron | 10.0% |
| !!! | ATTTGTATATTTCTAGTATT+AGG | - | chr7.1:8265557-8265576 | MS.gene22465:CDS | 15.0% |
| !! | AAGATATATTTAGTAGTAAG+AGG | + | chr7.1:8265989-8266008 | None:intergenic | 20.0% |
| !! | AATTTAAGAAATCTCATCTT+GGG | - | chr7.1:8265886-8265905 | MS.gene22465:CDS | 20.0% |
| !! | TGTAGTTTACATAGTATTAA+AGG | + | chr7.1:8266275-8266294 | None:intergenic | 20.0% |
| !!! | AATTTAGTTAATTTTAGGCA+TGG | - | chr7.1:8265719-8265738 | MS.gene22465:intron | 20.0% |
| ! | AATTAACATAACAGACTCAA+AGG | + | chr7.1:8266249-8266268 | None:intergenic | 25.0% |
| ! | AGGGTTCAATTAATCAAAAA+TGG | - | chr7.1:8266123-8266142 | MS.gene22465:intron | 25.0% |
| ! | CAATTTAAGAAATCTCATCT+TGG | - | chr7.1:8265885-8265904 | MS.gene22465:CDS | 25.0% |
| ! | GGGTTCAATTAATCAAAAAT+GGG | - | chr7.1:8266124-8266143 | MS.gene22465:intron | 25.0% |
| ! | TATGATGTTAAGGATTTGTA+TGG | - | chr7.1:8266040-8266059 | MS.gene22465:intron | 25.0% |
| ! | TTATTACGCATATGATGTTA+AGG | - | chr7.1:8266030-8266049 | MS.gene22465:intron | 25.0% |
| ! | TTCAAATACGACAATTAGAA+TGG | + | chr7.1:8265357-8265376 | None:intergenic | 25.0% |
| ! | TTTGAATTTGACTCTGAATT+TGG | - | chr7.1:8265371-8265390 | MS.gene22465:CDS | 25.0% |
| !!! | GAATTTTTTGCTACTTAGTA+TGG | - | chr7.1:8265493-8265512 | MS.gene22465:intron | 25.0% |
| AATTCAAGCAACCATCATTT+GGG | + | chr7.1:8266170-8266189 | None:intergenic | 30.0% | |
| ATCATCAACAACCATATTAC+TGG | + | chr7.1:8266309-8266328 | None:intergenic | 30.0% | |
| ATGTATATCCTCCAGTAATA+TGG | - | chr7.1:8266295-8266314 | MS.gene22465:intron | 30.0% | |
| GGAACACAAAAACATGAAAT+AGG | - | chr7.1:8265437-8265456 | MS.gene22465:intron | 30.0% | |
| TAATTCAAGCAACCATCATT+TGG | + | chr7.1:8266171-8266190 | None:intergenic | 30.0% | |
| ! | AGTAATATGGTTGTTGATGA+TGG | - | chr7.1:8266308-8266327 | MS.gene22465:intron | 30.0% |
| ! | ATTCGCTTCATTTGAAATCA+TGG | - | chr7.1:8265593-8265612 | MS.gene22465:CDS | 30.0% |
| ! | TTTGTTTATCTTTTGTCTGC+AGG | - | chr7.1:8265793-8265812 | MS.gene22465:CDS | 30.0% |
| ! | TTTTATGTGTAGTGGTATTG+AGG | - | chr7.1:8266065-8266084 | MS.gene22465:intron | 30.0% |
| AAGAACAATTCCCTTAGCAT+GGG | + | chr7.1:8265943-8265962 | None:intergenic | 35.0% | |
| AATCTCATCTTGGGAATGAA+TGG | - | chr7.1:8265895-8265914 | MS.gene22465:CDS | 35.0% | |
| ATCAACAACCATATTACTGG+AGG | + | chr7.1:8266306-8266325 | None:intergenic | 35.0% | |
| TGGTTGCTTGAATTACATTG+AGG | - | chr7.1:8266176-8266195 | MS.gene22465:intron | 35.0% | |
| ! | CTGTTTGCTTTCTGAGAATT+GGG | - | chr7.1:8265416-8265435 | MS.gene22465:intron | 35.0% |
| ! | GTATGGTCTTTTATGTGTAG+TGG | - | chr7.1:8266057-8266076 | MS.gene22465:intron | 35.0% |
| ! | TCTGTTTGCTTTCTGAGAAT+TGG | - | chr7.1:8265415-8265434 | MS.gene22465:intron | 35.0% |
| CAAGAACAATTCCCTTAGCA+TGG | + | chr7.1:8265944-8265963 | None:intergenic | 40.0% | |
| TCTTCTCCTTGAAAAGAGCA+AGG | + | chr7.1:8266439-8266458 | None:intergenic | 40.0% | |
| ! | GAATGGAAGAAACCCTTTTC+TGG | - | chr7.1:8265912-8265931 | MS.gene22465:CDS | 40.0% |
| !! | GGTTGTTGATGATGGTTTGT+AGG | - | chr7.1:8266316-8266335 | MS.gene22465:intron | 40.0% |
| !! | TGCTCTTTTCAAGGAGAAGA+AGG | - | chr7.1:8266439-8266458 | MS.gene22465:intron | 40.0% |
| AACCCTAGCACACTTACGAA+TGG | + | chr7.1:8266109-8266128 | None:intergenic | 45.0% | |
| ACACTTACGAATGGCAGAGT+TGG | + | chr7.1:8266100-8266119 | None:intergenic | 45.0% | |
| ACTCCGTATCAAGCAAAGGT+GGG | - | chr7.1:8265854-8265873 | MS.gene22465:CDS | 45.0% | |
| AGCATGGGATGAACCAGAAA+AGG | + | chr7.1:8265928-8265947 | None:intergenic | 45.0% | |
| ATCGCTGGATTTGGACGTAA+AGG | - | chr7.1:8266350-8266369 | MS.gene22465:intron | 45.0% | |
| CACTTACGAATGGCAGAGTT+GGG | + | chr7.1:8266099-8266118 | None:intergenic | 45.0% | |
| GAAGTCTTGATCGCTGGATT+TGG | - | chr7.1:8266341-8266360 | MS.gene22465:intron | 45.0% | |
| GCATGGGATGAACCAGAAAA+GGG | + | chr7.1:8265927-8265946 | None:intergenic | 45.0% | |
| TAGGATGAAGTCTTGATCGC+TGG | - | chr7.1:8266335-8266354 | MS.gene22465:intron | 45.0% | |
| TATTCCTGGAGTCAGGTTCA+AGG | - | chr7.1:8266388-8266407 | MS.gene22465:intron | 45.0% | |
| TGCAGGAAGACAAGAGGAAT+GGG | - | chr7.1:8265810-8265829 | MS.gene22465:CDS | 45.0% | |
| TGCCATTCGTAAGTGTGCTA+GGG | - | chr7.1:8266104-8266123 | MS.gene22465:intron | 45.0% | |
| TTCAAGGTCGTGAAGGTTTC+TGG | - | chr7.1:8266404-8266423 | MS.gene22465:intron | 45.0% | |
| TTTGTCTGCAGGAAGACAAG+AGG | - | chr7.1:8265804-8265823 | MS.gene22465:CDS | 45.0% | |
| ! | CTCTCTCCTTGCTCTTTTCA+AGG | - | chr7.1:8266430-8266449 | MS.gene22465:intron | 45.0% |
| ! | GCAACCATCATTTGGGACAA+AGG | + | chr7.1:8266163-8266182 | None:intergenic | 45.0% |
| ! | TCTGGTTCATCCCATGCTAA+GGG | - | chr7.1:8265930-8265949 | MS.gene22465:intron | 45.0% |
| ! | TTGGTGATATTCCTGGAGTC+AGG | - | chr7.1:8266381-8266400 | MS.gene22465:intron | 45.0% |
| !! | CATGCTGTTGGTGATATTCC+TGG | - | chr7.1:8266374-8266393 | MS.gene22465:intron | 45.0% |
| !! | TTCTGGTTCATCCCATGCTA+AGG | - | chr7.1:8265929-8265948 | MS.gene22465:intron | 45.0% |
| AGGAGAAGAAGGAGAAGCCT+AGG | - | chr7.1:8266450-8266469 | MS.gene22465:intron | 50.0% | |
| AGTCAGGTTCAAGGTCGTGA+AGG | - | chr7.1:8266397-8266416 | MS.gene22465:intron | 50.0% | |
| CAGCCCACCTTTGCTTGATA+CGG | + | chr7.1:8265860-8265879 | None:intergenic | 50.0% | |
| CTGCAGGAAGACAAGAGGAA+TGG | - | chr7.1:8265809-8265828 | MS.gene22465:CDS | 50.0% | |
| CTGCCATTCGTAAGTGTGCT+AGG | - | chr7.1:8266103-8266122 | MS.gene22465:intron | 50.0% | |
| GACTCCGTATCAAGCAAAGG+TGG | - | chr7.1:8265853-8265872 | MS.gene22465:CDS | 50.0% | |
| GCTGCCTTTGTCCCAAATGA+TGG | - | chr7.1:8266156-8266175 | MS.gene22465:intron | 50.0% | |
| GGACGTAAAGGTCATGCTGT+TGG | - | chr7.1:8266362-8266381 | MS.gene22465:intron | 50.0% | |
| GGAGACTCCGTATCAAGCAA+AGG | - | chr7.1:8265850-8265869 | MS.gene22465:CDS | 50.0% | |
| ACGACCTTGAACCTGACTCC+AGG | + | chr7.1:8266395-8266414 | None:intergenic | 55.0% | |
| TGGGAGCTGCTCGCAAGCTG+AGG | - | chr7.1:8265829-8265848 | MS.gene22465:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 8265281 | 8266478 | 8265281 | ID=MS.gene22465 |
| chr7.1 | mRNA | 8265281 | 8266478 | 8265281 | ID=MS.gene22465.t1;Parent=MS.gene22465 |
| chr7.1 | exon | 8266474 | 8266478 | 8266474 | ID=MS.gene22465.t1.exon1;Parent=MS.gene22465.t1 |
| chr7.1 | CDS | 8266474 | 8266478 | 8266474 | ID=cds.MS.gene22465.t1;Parent=MS.gene22465.t1 |
| chr7.1 | exon | 8265789 | 8265944 | 8265789 | ID=MS.gene22465.t1.exon2;Parent=MS.gene22465.t1 |
| chr7.1 | CDS | 8265789 | 8265944 | 8265789 | ID=cds.MS.gene22465.t1;Parent=MS.gene22465.t1 |
| chr7.1 | exon | 8265556 | 8265682 | 8265556 | ID=MS.gene22465.t1.exon3;Parent=MS.gene22465.t1 |
| chr7.1 | CDS | 8265556 | 8265682 | 8265556 | ID=cds.MS.gene22465.t1;Parent=MS.gene22465.t1 |
| chr7.1 | exon | 8265281 | 8265421 | 8265281 | ID=MS.gene22465.t1.exon4;Parent=MS.gene22465.t1 |
| chr7.1 | CDS | 8265281 | 8265421 | 8265281 | ID=cds.MS.gene22465.t1;Parent=MS.gene22465.t1 |
| Gene Sequence |
| Protein sequence |
