AlfalfaGEDB | MS.gene058560

M. sativa cultivar XinJiangDaYe / MS.gene058560

KEGG: K02960
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00380.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058560.t1	XP_003631001.1	100	140	0	0	1	140	1	140	2.40E-73	284.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058560.t1	P46293	94.3	140	8	0	1	140	6	145	1.2e-70	266.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058560.t1	G7LBJ0	100.0	140	0	0	1	140	1	140	1.7e-73	284.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene054937	MS.gene058560	0.816496	5.37E-52	-1.69E-46
MS.gene057052	MS.gene058560	0.801049	1.08E-48	-1.69E-46
MS.gene058560	MS.gene22465	0.81253	4.05E-51	-1.69E-46
MS.gene058560	MS.gene39764	0.803968	2.71E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene015616	MS.gene058560	PPI
MS.gene34284	MS.gene058560	PPI
MS.gene034319	MS.gene058560	PPI
MS.gene62652	MS.gene058560	PPI
MS.gene054733	MS.gene058560	PPI
MS.gene058560	MS.gene77759	PPI
MS.gene50088	MS.gene058560	PPI
MS.gene034318	MS.gene058560	PPI
MS.gene98113	MS.gene058560	PPI
MS.gene063979	MS.gene058560	PPI
MS.gene34285	MS.gene058560	PPI
MS.gene95856	MS.gene058560	PPI
MS.gene34283	MS.gene058560	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058560.t1	MTR_8g106080	100.000	140	0	1	140	1	140	1.17e-100	285
MS.gene058560.t1	MTR_8g106020	100.000	140	0	1	140	1	140	1.17e-100	285
MS.gene058560.t1	MTR_5g075240	99.286	140	1	1	140	1	140	2.63e-100	283
MS.gene058560.t1	MTR_4g085580	95.000	140	7	1	140	4	143	2.91e-96	273

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058560.t1	AT2G09990	89.928	139	14	2	140	8	146	5.16e-92	263
MS.gene058560.t1	AT5G18380	89.209	139	15	2	140	8	146	8.83e-92	262
MS.gene058560.t1	AT3G04230	84.173	139	22	2	140	8	146	1.84e-85	246
MS.gene058560.t1	AT5G18380	87.200	125	16	2	126	8	132	3.68e-79	230
MS.gene058560.t1	AT5G18380	87.037	108	14	2	109	8	115	1.14e-67	201

Find 40 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCTAACGATAAGATTTC+TGG	0.210396	4.4:+1772165	None:intergenic
ACAGAGTAAGAAGGAGATTA+AGG	0.258952	4.4:-1772282	MS.gene058560:CDS
CGCTGTGAGCCTAAGAAGTT+TGG	0.359075	4.4:-1772212	MS.gene058560:CDS
CTGCAACAGCGTTCTTCTTC+CGG	0.375769	4.4:+1772546	None:intergenic
TCAGAGTATTGCTAAGGCTT+TGG	0.386737	4.4:-1772333	MS.gene058560:CDS
GCCTGAGATTCTTCGTTTCA+AGG	0.392228	4.4:-1772459	MS.gene058560:CDS
CCACGACCACCAAACTTCTT+AGG	0.436009	4.4:+1772203	None:intergenic
TACGAGCCAATCCTCCTTCT+CGG	0.440837	4.4:-1772434	MS.gene058560:CDS
TCTGACGAATGGCATAGATC+TGG	0.445614	4.4:+1772351	None:intergenic
ATGGAACAGGTGCAATGCTT+TGG	0.453443	4.4:-1772569	MS.gene058560:CDS
GCCTTGAAACGAAGAATCTC+AGG	0.478888	4.4:+1772458	None:intergenic
GAAGCTCAGCAAGATGGAAC+AGG	0.488449	4.4:-1772582	None:intergenic
CGCGGTCTCATCAAGATCAA+TGG	0.494184	4.4:-1772503	MS.gene058560:CDS
TCATATCAACTCCGGCGAAA+CGG	0.494924	4.4:+1772405	None:intergenic
TCAGGCTCCACGAGTTCGAT+TGG	0.496656	4.4:+1772476	None:intergenic
GTGGTCGTGGTGCTCGTGCT+AGG	0.502073	4.4:-1772190	MS.gene058560:CDS
CTCGGAAGACACCGTTTCGC+CGG	0.504037	4.4:-1772416	MS.gene058560:CDS
GTTTCGCCGGAGTTGATATG+AGG	0.511168	4.4:-1772403	MS.gene058560:CDS
CCTAAGAAGTTTGGTGGTCG+TGG	0.511400	4.4:-1772203	MS.gene058560:CDS
AGGATCCGTGTGAAGGGAGG+AGG	0.521481	4.4:-1772383	MS.gene058560:CDS
CTGACGAATGGCATAGATCT+GGG	0.534090	4.4:+1772352	None:intergenic
TGTTGATGAACAGAGTAAGA+AGG	0.534508	4.4:-1772291	MS.gene058560:CDS
AACAGGTGCAATGCTTTGGC+CGG	0.546867	4.4:-1772565	MS.gene058560:CDS
AACGGTGTCTTCCGAGAAGG+AGG	0.549270	4.4:+1772423	None:intergenic
TGTGAGCCTAAGAAGTTTGG+TGG	0.558448	4.4:-1772209	MS.gene058560:CDS
ACCCACTGCAAGAGAGGTCG+CGG	0.562945	4.4:-1772521	MS.gene058560:CDS
TGATATGAGGATCCGTGTGA+AGG	0.569818	4.4:-1772390	MS.gene058560:CDS
GCAGTTACCCACTGCAAGAG+AGG	0.580125	4.4:-1772527	MS.gene058560:CDS
CATTCGTCAGAGTATTGCTA+AGG	0.581266	4.4:-1772339	MS.gene058560:CDS
TGTCTTCCGAGAAGGAGGAT+TGG	0.582501	4.4:+1772428	None:intergenic
TGGCTGTCCAATCGAACTCG+TGG	0.587642	4.4:-1772483	MS.gene058560:CDS
TTCTTAGGCTCACAGCGTCT+AGG	0.590494	4.4:+1772218	None:intergenic
GATATGAGGATCCGTGTGAA+GGG	0.594162	4.4:-1772389	MS.gene058560:CDS
CTTAGCAATACTCTGACGAA+TGG	0.613778	4.4:+1772340	None:intergenic
ATGAGGATCCGTGTGAAGGG+AGG	0.615322	4.4:-1772386	MS.gene058560:CDS
ACCGCGACCTCTCTTGCAGT+GGG	0.618077	4.4:+1772520	None:intergenic
CGAAACGGTGTCTTCCGAGA+AGG	0.622095	4.4:+1772420	None:intergenic
GACCGCGACCTCTCTTGCAG+TGG	0.624398	4.4:+1772519	None:intergenic
TGTGACCTCCTCCCTTCACA+CGG	0.629480	4.4:+1772378	None:intergenic
ACGGATCCTCATATCAACTC+CGG	0.648153	4.4:+1772397	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ACAGAGTAAGAAGGAGATTA+AGG	-	chr4.4:1772456-1772475	MS.gene058560:CDS	35.0%
!	TGTTGATGAACAGAGTAAGA+AGG	-	chr4.4:1772447-1772466	MS.gene058560:CDS	35.0%
	CTTAGCAATACTCTGACGAA+TGG	+	chr4.4:1772401-1772420	None:intergenic	40.0%
!!	CATTCGTCAGAGTATTGCTA+AGG	-	chr4.4:1772399-1772418	MS.gene058560:CDS	40.0%
!!	TCAGAGTATTGCTAAGGCTT+TGG	-	chr4.4:1772405-1772424	MS.gene058560:CDS	40.0%
	ACGGATCCTCATATCAACTC+CGG	+	chr4.4:1772344-1772363	None:intergenic	45.0%
	ATGGAACAGGTGCAATGCTT+TGG	-	chr4.4:1772169-1772188	MS.gene058560:CDS	45.0%
	CTGACGAATGGCATAGATCT+GGG	+	chr4.4:1772389-1772408	None:intergenic	45.0%
	GATATGAGGATCCGTGTGAA+GGG	-	chr4.4:1772349-1772368	MS.gene058560:CDS	45.0%
	GCCTGAGATTCTTCGTTTCA+AGG	-	chr4.4:1772279-1772298	MS.gene058560:CDS	45.0%
	GCCTTGAAACGAAGAATCTC+AGG	+	chr4.4:1772283-1772302	None:intergenic	45.0%
	TCATATCAACTCCGGCGAAA+CGG	+	chr4.4:1772336-1772355	None:intergenic	45.0%
	TCTGACGAATGGCATAGATC+TGG	+	chr4.4:1772390-1772409	None:intergenic	45.0%
	TGATATGAGGATCCGTGTGA+AGG	-	chr4.4:1772348-1772367	MS.gene058560:CDS	45.0%
	TGTGAGCCTAAGAAGTTTGG+TGG	-	chr4.4:1772529-1772548	MS.gene058560:CDS	45.0%
	AACAGGTGCAATGCTTTGGC+CGG	-	chr4.4:1772173-1772192	MS.gene058560:CDS	50.0%
	CCACGACCACCAAACTTCTT+AGG	+	chr4.4:1772538-1772557	None:intergenic	50.0%
	CGCGGTCTCATCAAGATCAA+TGG	-	chr4.4:1772235-1772254	MS.gene058560:CDS	50.0%
	CGCTGTGAGCCTAAGAAGTT+TGG	-	chr4.4:1772526-1772545	MS.gene058560:CDS	50.0%
	CTGCAACAGCGTTCTTCTTC+CGG	+	chr4.4:1772195-1772214	None:intergenic	50.0%
	TACGAGCCAATCCTCCTTCT+CGG	-	chr4.4:1772304-1772323	MS.gene058560:CDS	50.0%
	TGTCTTCCGAGAAGGAGGAT+TGG	+	chr4.4:1772313-1772332	None:intergenic	50.0%
	TTCTTAGGCTCACAGCGTCT+AGG	+	chr4.4:1772523-1772542	None:intergenic	50.0%
!	CCTAAGAAGTTTGGTGGTCG+TGG	-	chr4.4:1772535-1772554	MS.gene058560:CDS	50.0%
!!	GTTTCGCCGGAGTTGATATG+AGG	-	chr4.4:1772335-1772354	MS.gene058560:CDS	50.0%
	AACGGTGTCTTCCGAGAAGG+AGG	+	chr4.4:1772318-1772337	None:intergenic	55.0%
	ATGAGGATCCGTGTGAAGGG+AGG	-	chr4.4:1772352-1772371	MS.gene058560:CDS	55.0%
	CGAAACGGTGTCTTCCGAGA+AGG	+	chr4.4:1772321-1772340	None:intergenic	55.0%
	GCAGTTACCCACTGCAAGAG+AGG	-	chr4.4:1772211-1772230	MS.gene058560:CDS	55.0%
	TCAGGCTCCACGAGTTCGAT+TGG	+	chr4.4:1772265-1772284	None:intergenic	55.0%
	TGGCTGTCCAATCGAACTCG+TGG	-	chr4.4:1772255-1772274	MS.gene058560:CDS	55.0%
	TGTGACCTCCTCCCTTCACA+CGG	+	chr4.4:1772363-1772382	None:intergenic	55.0%
	ACCCACTGCAAGAGAGGTCG+CGG	-	chr4.4:1772217-1772236	MS.gene058560:CDS	60.0%
	ACCGCGACCTCTCTTGCAGT+GGG	+	chr4.4:1772221-1772240	None:intergenic	60.0%
	AGGATCCGTGTGAAGGGAGG+AGG	-	chr4.4:1772355-1772374	MS.gene058560:CDS	60.0%
	CTCGGAAGACACCGTTTCGC+CGG	-	chr4.4:1772322-1772341	MS.gene058560:CDS	60.0%
	GACCGCGACCTCTCTTGCAG+TGG	+	chr4.4:1772222-1772241	None:intergenic	65.0%
	GTGGTCGTGGTGCTCGTGCT+AGG	-	chr4.4:1772548-1772567	MS.gene058560:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	1772169	1772591	1772169	ID=MS.gene058560
chr4.4	mRNA	1772169	1772591	1772169	ID=MS.gene058560.t1;Parent=MS.gene058560
chr4.4	exon	1772169	1772591	1772169	ID=MS.gene058560.t1.exon1;Parent=MS.gene058560.t1
chr4.4	CDS	1772169	1772591	1772169	ID=cds.MS.gene058560.t1;Parent=MS.gene058560.t1

Gene Sequence

>MS.gene058560

ATGGAACAGGTGCAATGCTTTGGCCGGAAGAAGAACGCTGTTGCAGTTACCCACTGCAAGAGAGGTCGCGGTCTCATCAAGATCAATGGCTGTCCAATCGAACTCGTGGAGCCTGAGATTCTTCGTTTCAAGGCATACGAGCCAATCCTCCTTCTCGGAAGACACCGTTTCGCCGGAGTTGATATGAGGATCCGTGTGAAGGGAGGAGGTCACACTTCCCAGATCTATGCCATTCGTCAGAGTATTGCTAAGGCTTTGGTTGCTTATTATCAGAAATATGTTGATGAACAGAGTAAGAAGGAGATTAAGGATATTCTTGTTCGTTATGATCGTACTCTTCTTGTTGCTGATCCTAGACGCTGTGAGCCTAAGAAGTTTGGTGGTCGTGGTGCTCGTGCTAGGTTCCAGAAATCTTATCGTTAG

Protein sequence

>MS.gene058560.t1

MEQVQCFGRKKNAVAVTHCKRGRGLIKINGCPIELVEPEILRFKAYEPILLLGRHRFAGVDMRIRVKGGGHTSQIYAIRQSIAKALVAYYQKYVDEQSKKEIKDILVRYDRTLLVADPRRCEPKKFGGRGARARFQKSYR