Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22611.t1 | XP_024632552.1 | 54.8 | 126 | 12 | 1 | 1 | 81 | 257 | 382 | 1.00E-18 | 102.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22611.t1 | Q9SYD6 | 73.8 | 65 | 17 | 0 | 17 | 81 | 326 | 390 | 4.1e-16 | 85.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22611.t1 | G7IRZ8 | 54.8 | 126 | 12 | 1 | 1 | 81 | 257 | 382 | 7.5e-19 | 102.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049587 | MS.gene22611 | 0.801917 | 7.18E-49 | -1.69E-46 |
| MS.gene051710 | MS.gene22611 | 0.804529 | 2.07E-49 | -1.69E-46 |
| MS.gene053974 | MS.gene22611 | 0.820012 | 8.59E-53 | -1.69E-46 |
| MS.gene054801 | MS.gene22611 | 0.819562 | 1.09E-52 | -1.69E-46 |
| MS.gene055058 | MS.gene22611 | 0.805988 | 1.02E-49 | -1.69E-46 |
| MS.gene058515 | MS.gene22611 | 0.831612 | 1.51E-55 | -1.69E-46 |
| MS.gene059276 | MS.gene22611 | 0.837851 | 4.07E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22611.t1 | MTR_2g097900 | 54.762 | 126 | 12 | 1 | 1 | 81 | 257 | 382 | 5.12e-31 | 113 |
| MS.gene22611.t1 | MTR_3g029510 | 63.077 | 65 | 24 | 0 | 17 | 81 | 347 | 411 | 1.19e-18 | 79.0 |
| MS.gene22611.t1 | MTR_8g037170 | 55.696 | 79 | 34 | 1 | 4 | 81 | 349 | 427 | 4.48e-18 | 77.4 |
| MS.gene22611.t1 | MTR_8g037170 | 55.696 | 79 | 34 | 1 | 4 | 81 | 336 | 414 | 4.69e-18 | 77.4 |
| MS.gene22611.t1 | MTR_8g037175 | 38.889 | 126 | 32 | 1 | 1 | 81 | 277 | 402 | 2.29e-17 | 75.1 |
| MS.gene22611.t1 | MTR_8g036660 | 56.410 | 78 | 33 | 1 | 5 | 81 | 405 | 482 | 1.35e-16 | 73.2 |
| MS.gene22611.t1 | MTR_2g097900 | 46.078 | 102 | 10 | 1 | 1 | 57 | 257 | 358 | 9.04e-16 | 70.5 |
| MS.gene22611.t1 | MTR_7g070210 | 41.791 | 67 | 39 | 0 | 15 | 81 | 369 | 435 | 6.48e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22611.t1 | AT1G51340 | 73.846 | 65 | 17 | 0 | 17 | 81 | 326 | 390 | 1.17e-23 | 92.4 |
| MS.gene22611.t1 | AT1G51340 | 72.727 | 66 | 18 | 0 | 16 | 81 | 319 | 384 | 2.49e-23 | 92.0 |
| MS.gene22611.t1 | AT1G51340 | 72.727 | 66 | 18 | 0 | 16 | 81 | 325 | 390 | 2.75e-23 | 92.0 |
| MS.gene22611.t1 | AT1G51340 | 72.727 | 66 | 18 | 0 | 16 | 81 | 325 | 390 | 2.75e-23 | 92.0 |
| MS.gene22611.t1 | AT1G51340 | 72.727 | 66 | 18 | 0 | 16 | 81 | 325 | 390 | 2.75e-23 | 92.0 |
| MS.gene22611.t1 | AT3G08040 | 62.500 | 64 | 24 | 0 | 18 | 81 | 227 | 290 | 2.13e-18 | 78.2 |
| MS.gene22611.t1 | AT3G08040 | 62.500 | 64 | 24 | 0 | 18 | 81 | 331 | 394 | 3.07e-18 | 77.8 |
| MS.gene22611.t1 | AT2G38330 | 44.776 | 67 | 37 | 0 | 15 | 81 | 324 | 390 | 6.19e-12 | 59.7 |
Find 21 sgRNAs with CRISPR-Local
Find 92 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAAGTCTTTCTGCAGGTT+TGG | 0.310099 | 5.1:+4463463 | MS.gene22611:CDS |
| TTGCAGGATTTCTATTACAC+TGG | 0.383898 | 5.1:+4463395 | MS.gene22611:intron |
| AACTGCTACTCGAGTATTGC+AGG | 0.384369 | 5.1:+4463661 | MS.gene22611:CDS |
| GTCCCTTCTTGCGGATGGTC+TGG | 0.395512 | 5.1:+4463494 | MS.gene22611:CDS |
| TGCATTTCAAGTCTTTCTGC+AGG | 0.411677 | 5.1:+4463458 | MS.gene22611:CDS |
| TGGTCTGGCTGTTGCCGAGC+AGG | 0.466428 | 5.1:+4463509 | MS.gene22611:CDS |
| AGTCTTTCTGCAGGTTTGGT+TGG | 0.483937 | 5.1:+4463467 | MS.gene22611:CDS |
| TGCAGATGCATTTGCTAACA+AGG | 0.487695 | 5.1:+4463619 | MS.gene22611:CDS |
| GCAGTGTCCCTTCTTGCGGA+TGG | 0.503081 | 5.1:+4463489 | MS.gene22611:CDS |
| AAGGAAGTGCATGATTATTG+AGG | 0.533508 | 5.1:+4464514 | MS.gene22611:intron |
| CAGCCAGACCATCCGCAAGA+AGG | 0.542007 | 5.1:-4463497 | None:intergenic |
| TTGCTAACAAGGACTATGAA+AGG | 0.543442 | 5.1:+4463630 | MS.gene22611:CDS |
| TGCTAACAAGGACTATGAAA+GGG | 0.543836 | 5.1:+4463631 | MS.gene22611:CDS |
| CTGAGATATAACATGTGTAA+TGG | 0.565189 | 5.1:-4462901 | None:intergenic |
| GTTGGCAGTGTCCCTTCTTG+CGG | 0.568099 | 5.1:+4463485 | MS.gene22611:CDS |
| AGCCAGACCATCCGCAAGAA+GGG | 0.579779 | 5.1:-4463496 | None:intergenic |
| TACTCGAGTAGCAGTTGTTG+AGG | 0.609873 | 5.1:-4463654 | None:intergenic |
| TGAATCATATCTCACCTGCT+CGG | 0.614688 | 5.1:-4463523 | None:intergenic |
| AAAGACTTGAAATGCAGCCA+CGG | 0.621083 | 5.1:-4463451 | None:intergenic |
| CAGCCACGGATGTTGCTCTG+TGG | 0.705573 | 5.1:-4463437 | None:intergenic |
| ACACCACAGAGCAACATCCG+TGG | 0.710654 | 5.1:+4463434 | MS.gene22611:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATAATTATCAAAACTTTAA+TGG | + | chr5.1:4464441-4464460 | MS.gene22611:intron | 10.0% |
| !! | TATAGTTTAATACATATTTA+AGG | + | chr5.1:4462958-4462977 | MS.gene22611:intron | 10.0% |
| !! | AAAAAAATACATGATAACAT+TGG | + | chr5.1:4463956-4463975 | MS.gene22611:intron | 15.0% |
| !! | AAAATAGAACATACAAAAAA+GGG | - | chr5.1:4464219-4464238 | None:intergenic | 15.0% |
| !! | CACAAATTAAAATAAGAAAA+AGG | - | chr5.1:4464343-4464362 | None:intergenic | 15.0% |
| !!! | AAATTGTTCAATGTTATTTT+AGG | + | chr5.1:4463261-4463280 | MS.gene22611:intron | 15.0% |
| !! | AAATAGAACATACAAAAAAG+GGG | - | chr5.1:4464218-4464237 | None:intergenic | 20.0% |
| !! | GAAAATAGAACATACAAAAA+AGG | - | chr5.1:4464220-4464239 | None:intergenic | 20.0% |
| !! | TGTTCATTATTCCAATTTAT+TGG | + | chr5.1:4464185-4464204 | MS.gene22611:intron | 20.0% |
| !! | TTAGATATCAAAAATAACTG+TGG | + | chr5.1:4464282-4464301 | MS.gene22611:intron | 20.0% |
| !! | TTATGCTTATATATACGAAA+TGG | + | chr5.1:4463185-4463204 | MS.gene22611:intron | 20.0% |
| !! | TTCAAATTAACTGTAAATGT+AGG | + | chr5.1:4463051-4463070 | MS.gene22611:intron | 20.0% |
| !!! | TAATAAACCTTTACCATTTT+TGG | - | chr5.1:4463168-4463187 | None:intergenic | 20.0% |
| !!! | TATCTAAAATTCAATTCCAA+TGG | - | chr5.1:4464269-4464288 | None:intergenic | 20.0% |
| !!! | TTTAGGTTTATACACAAATT+TGG | + | chr5.1:4463278-4463297 | MS.gene22611:intron | 20.0% |
| ! | AATTCCAATGGATAATTAAG+TGG | - | chr5.1:4464257-4464276 | None:intergenic | 25.0% |
| ! | ATATCAAAAATAACTGTGGA+TGG | + | chr5.1:4464286-4464305 | MS.gene22611:intron | 25.0% |
| ! | CAAAATCAAAAAGAAGCAAA+AGG | - | chr5.1:4463019-4463038 | None:intergenic | 25.0% |
| ! | CAATATAATAACAAAGTGTG+TGG | - | chr5.1:4463876-4463895 | None:intergenic | 25.0% |
| ! | TAAACTAATAGTATCTCTAG+TGG | - | chr5.1:4464109-4464128 | None:intergenic | 25.0% |
| ! | TTAAGTGGCTAATTTCAATA+CGG | - | chr5.1:4464242-4464261 | None:intergenic | 25.0% |
| ! | TTGCAAGTATTCTATCTATT+TGG | + | chr5.1:4464417-4464436 | MS.gene22611:intron | 25.0% |
| !! | ATATCTCAGTATGTTTTACT+TGG | + | chr5.1:4462915-4462934 | MS.gene22611:intron | 25.0% |
| !! | TTGATTTTGCTTCTTTGTAA+AGG | + | chr5.1:4464495-4464514 | MS.gene22611:intron | 25.0% |
| !!! | AAACCAAGATGATGTTTTTT+CGG | - | chr5.1:4463588-4463607 | None:intergenic | 25.0% |
| AAAAACATCATCTTGGTTTC+AGG | + | chr5.1:4463589-4463608 | MS.gene22611:intron | 30.0% | |
| AAACAACACTAACTAAAGCA+AGG | - | chr5.1:4464077-4464096 | None:intergenic | 30.0% | |
| ACTACCAATTTGAACGAAAA+AGG | - | chr5.1:4463342-4463361 | None:intergenic | 30.0% | |
| AGGAAATTAAGAGACAAGAT+AGG | - | chr5.1:4462999-4463018 | None:intergenic | 30.0% | |
| CTGAGATATAACATGTGTAA+TGG | - | chr5.1:4462904-4462923 | None:intergenic | 30.0% | |
| GATATGATTCATTACTCTCT+CGG | + | chr5.1:4463535-4463554 | MS.gene22611:intron | 30.0% | |
| TTAGCCACTTAATTATCCAT+TGG | + | chr5.1:4464250-4464269 | MS.gene22611:intron | 30.0% | |
| TTCCTTAAACAAAGGAAAGA+TGG | - | chr5.1:4463788-4463807 | None:intergenic | 30.0% | |
| TTTAAATTCTGCGACTAGAT+TGG | + | chr5.1:4464020-4464039 | MS.gene22611:intron | 30.0% | |
| TTTGTTTCGATAATATCCCT+AGG | - | chr5.1:4463244-4463263 | None:intergenic | 30.0% | |
| ! | AATTGCATATGTTTGTTGCT+TGG | - | chr5.1:4463134-4463153 | None:intergenic | 30.0% |
| !! | TACTCCTTTTTCGTTCAAAT+TGG | + | chr5.1:4463335-4463354 | MS.gene22611:intron | 30.0% |
| !!! | CATTATTTTAAGAAGATCGC+TGG | + | chr5.1:4463691-4463710 | MS.gene22611:intron | 30.0% |
| !!! | CTTTACCATTTTTGGCAAAT+CGG | - | chr5.1:4463160-4463179 | None:intergenic | 30.0% |
| !!! | TCGCTGGTTCTTTTTATAAA+TGG | + | chr5.1:4463707-4463726 | MS.gene22611:intron | 30.0% |
| AAGACAATAATGAAACGGGA+AGG | - | chr5.1:4463377-4463396 | None:intergenic | 35.0% | |
| AAGTTGTAGCTAGAGTTACT+TGG | - | chr5.1:4463740-4463759 | None:intergenic | 35.0% | |
| AATAACAAAGTGTGTGGTGT+AGG | - | chr5.1:4463870-4463889 | None:intergenic | 35.0% | |
| ATAACAAAGTGTGTGGTGTA+GGG | - | chr5.1:4463869-4463888 | None:intergenic | 35.0% | |
| ATGCATAGCTTAATCAGCAT+TGG | + | chr5.1:4463901-4463920 | MS.gene22611:intron | 35.0% | |
| CCTGCAAGACAATAATGAAA+CGG | - | chr5.1:4463382-4463401 | None:intergenic | 35.0% | |
| CGATTTGCCAAAAATGGTAA+AGG | + | chr5.1:4463158-4463177 | MS.gene22611:intron | 35.0% | |
| CTGCAAGACAATAATGAAAC+GGG | - | chr5.1:4463381-4463400 | None:intergenic | 35.0% | |
| CTTCCGAAAAAACATCATCT+TGG | + | chr5.1:4463582-4463601 | MS.gene22611:intron | 35.0% | |
| TAGAACATACAAAAAAGGGG+AGG | - | chr5.1:4464215-4464234 | None:intergenic | 35.0% | |
| TAGCAGAGATATACTTCCTA+GGG | + | chr5.1:4463225-4463244 | MS.gene22611:intron | 35.0% | |
| TGCTAACAAGGACTATGAAA+GGG | + | chr5.1:4463631-4463650 | MS.gene22611:CDS | 35.0% | |
| TTAGCAGAGATATACTTCCT+AGG | + | chr5.1:4463224-4463243 | MS.gene22611:intron | 35.0% | |
| TTGCAGGATTTCTATTACAC+TGG | + | chr5.1:4463395-4463414 | MS.gene22611:intron | 35.0% | |
| TTGCTAACAAGGACTATGAA+AGG | + | chr5.1:4463630-4463649 | MS.gene22611:CDS | 35.0% | |
| TTGGGAACTTCCTTAAACAA+AGG | - | chr5.1:4463796-4463815 | None:intergenic | 35.0% | |
| ! | GTAACTCTAGCTACAACTTT+TGG | + | chr5.1:4463741-4463760 | MS.gene22611:intron | 35.0% |
| ! | GTCCATCTTTCCTTTGTTTA+AGG | + | chr5.1:4463783-4463802 | MS.gene22611:intron | 35.0% |
| AAAGACTTGAAATGCAGCCA+CGG | - | chr5.1:4463454-4463473 | None:intergenic | 40.0% | |
| CCGTTTCATTATTGTCTTGC+AGG | + | chr5.1:4463379-4463398 | MS.gene22611:intron | 40.0% | |
| CTTGTTTGTTCAAACTCCAC+AGG | - | chr5.1:4463097-4463116 | None:intergenic | 40.0% | |
| TAACAAAGTGTGTGGTGTAG+GGG | - | chr5.1:4463868-4463887 | None:intergenic | 40.0% | |
| TACTTTCAGCTATACTCCTG+TGG | + | chr5.1:4463078-4463097 | MS.gene22611:intron | 40.0% | |
| TCTCCAAAACACGCACAAAT+TGG | + | chr5.1:4463841-4463860 | MS.gene22611:intron | 40.0% | |
| TGAACGAAAAAGGAGTAGCA+CGG | - | chr5.1:4463332-4463351 | None:intergenic | 40.0% | |
| TGAATCATATCTCACCTGCT+CGG | - | chr5.1:4463526-4463545 | None:intergenic | 40.0% | |
| TTCAAGTCTTTCTGCAGGTT+TGG | + | chr5.1:4463463-4463482 | MS.gene22611:CDS | 40.0% | |
| ! | TGCAGATGCATTTGCTAACA+AGG | + | chr5.1:4463619-4463638 | MS.gene22611:CDS | 40.0% |
| ! | TGCATATGTTTGTTGCTTGG+AGG | - | chr5.1:4463131-4463150 | None:intergenic | 40.0% |
| ! | TGCATTTCAAGTCTTTCTGC+AGG | + | chr5.1:4463458-4463477 | MS.gene22611:CDS | 40.0% |
| !! | TTCGACCGATTTGCCAAAAA+TGG | + | chr5.1:4463152-4463171 | MS.gene22611:intron | 40.0% |
| !!! | CGTGTTTTGGAGATTTAAGG+TGG | - | chr5.1:4463834-4463853 | None:intergenic | 40.0% |
| !!! | GTGCGTGTTTTGGAGATTTA+AGG | - | chr5.1:4463837-4463856 | None:intergenic | 40.0% |
| !!! | GTTTTGGAGATTTAAGGTGG+TGG | - | chr5.1:4463831-4463850 | None:intergenic | 40.0% |
| ACTGTGGATGGAACTATCAC+AGG | + | chr5.1:4464298-4464317 | MS.gene22611:intron | 45.0% | |
| GAAACGGGAAGGACAATCAA+TGG | - | chr5.1:4463366-4463385 | None:intergenic | 45.0% | |
| TACTCGAGTAGCAGTTGTTG+AGG | - | chr5.1:4463657-4463676 | None:intergenic | 45.0% | |
| ! | AACTGCTACTCGAGTATTGC+AGG | + | chr5.1:4463661-4463680 | MS.gene22611:CDS | 45.0% |
| ! | AGTCTTTCTGCAGGTTTGGT+TGG | + | chr5.1:4463467-4463486 | MS.gene22611:CDS | 45.0% |
| ! | GGACCAATTTGTGCGTGTTT+TGG | - | chr5.1:4463847-4463866 | None:intergenic | 45.0% |
| ! | TTGGAGATTTAAGGTGGTGG+TGG | - | chr5.1:4463828-4463847 | None:intergenic | 45.0% |
| GGGGAGGCTCTCCAATAAAT+TGG | - | chr5.1:4464199-4464218 | None:intergenic | 50.0% | |
| ACACCACAGAGCAACATCCG+TGG | + | chr5.1:4463434-4463453 | MS.gene22611:CDS | 55.0% | |
| AGCCAGACCATCCGCAAGAA+GGG | - | chr5.1:4463499-4463518 | None:intergenic | 55.0% | |
| GTTGGCAGTGTCCCTTCTTG+CGG | + | chr5.1:4463485-4463504 | MS.gene22611:CDS | 55.0% | |
| !! | TGGTGGTGGAGCAGCTACTT+GGG | - | chr5.1:4463814-4463833 | None:intergenic | 55.0% |
| CAGCCACGGATGTTGCTCTG+TGG | - | chr5.1:4463440-4463459 | None:intergenic | 60.0% | |
| CAGCCAGACCATCCGCAAGA+AGG | - | chr5.1:4463500-4463519 | None:intergenic | 60.0% | |
| GCAGTGTCCCTTCTTGCGGA+TGG | + | chr5.1:4463489-4463508 | MS.gene22611:CDS | 60.0% | |
| GTCCCTTCTTGCGGATGGTC+TGG | + | chr5.1:4463494-4463513 | MS.gene22611:CDS | 60.0% | |
| !! | GTGGTGGTGGAGCAGCTACT+TGG | - | chr5.1:4463815-4463834 | None:intergenic | 60.0% |
| TGGTCTGGCTGTTGCCGAGC+AGG | + | chr5.1:4463509-4463528 | MS.gene22611:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 4462882 | 4464534 | 4462882 | ID=MS.gene22611 |
| chr5.1 | mRNA | 4462882 | 4464534 | 4462882 | ID=MS.gene22611.t1;Parent=MS.gene22611 |
| chr5.1 | exon | 4462882 | 4462922 | 4462882 | ID=MS.gene22611.t1.exon1;Parent=MS.gene22611.t1 |
| chr5.1 | CDS | 4462882 | 4462922 | 4462882 | ID=cds.MS.gene22611.t1;Parent=MS.gene22611.t1 |
| chr5.1 | exon | 4463401 | 4463530 | 4463401 | ID=MS.gene22611.t1.exon2;Parent=MS.gene22611.t1 |
| chr5.1 | CDS | 4463401 | 4463530 | 4463401 | ID=cds.MS.gene22611.t1;Parent=MS.gene22611.t1 |
| chr5.1 | exon | 4463611 | 4463682 | 4463611 | ID=MS.gene22611.t1.exon3;Parent=MS.gene22611.t1 |
| chr5.1 | CDS | 4463611 | 4463682 | 4463611 | ID=cds.MS.gene22611.t1;Parent=MS.gene22611.t1 |
| chr5.1 | exon | 4464517 | 4464534 | 4464517 | ID=MS.gene22611.t1.exon4;Parent=MS.gene22611.t1 |
| chr5.1 | CDS | 4464517 | 4464534 | 4464517 | ID=cds.MS.gene22611.t1;Parent=MS.gene22611.t1 |
| Gene Sequence |
| Protein sequence |
