Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049587.t1 | KEH42439.1 | 94 | 50 | 3 | 0 | 3 | 52 | 65 | 114 | 2.00E-16 | 94.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049587.t1 | A0A072VWJ0 | 94.0 | 50 | 3 | 0 | 3 | 52 | 116 | 165 | 1.4e-16 | 94.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049587 | MS.gene058515 | 0.818853 | 1.58E-52 | -1.69E-46 |
| MS.gene049587 | MS.gene06269 | 0.807876 | 4.08E-50 | -1.69E-46 |
| MS.gene049587 | MS.gene064075 | 0.806339 | 8.63E-50 | -1.69E-46 |
| MS.gene049587 | MS.gene068453 | 0.800499 | 1.40E-48 | -1.69E-46 |
| MS.gene049587 | MS.gene22611 | 0.801917 | 7.18E-49 | -1.69E-46 |
| MS.gene049587 | MS.gene23940 | 0.81838 | 2.02E-52 | -1.69E-46 |
| MS.gene049587 | MS.gene25778 | 0.803262 | 3.79E-49 | -1.69E-46 |
| MS.gene049587 | MS.gene26056 | 0.835913 | 1.27E-56 | -1.69E-46 |
| MS.gene049587 | MS.gene30073 | 0.812463 | 4.19E-51 | -1.69E-46 |
| MS.gene049587 | MS.gene38429 | 0.808816 | 2.57E-50 | -1.69E-46 |
| MS.gene049587 | MS.gene38850 | 0.811518 | 6.73E-51 | -1.69E-46 |
| MS.gene049587 | MS.gene42573 | 0.809002 | 2.35E-50 | -1.69E-46 |
| MS.gene049587 | MS.gene42887 | 0.800095 | 1.69E-48 | -1.69E-46 |
| MS.gene049587 | MS.gene42891 | 0.801895 | 7.26E-49 | -1.69E-46 |
| MS.gene049587 | MS.gene59780 | 0.826658 | 2.40E-54 | -1.69E-46 |
| MS.gene049587 | MS.gene61742 | 0.81542 | 9.33E-52 | -1.69E-46 |
| MS.gene049587 | MS.gene62748 | 0.825078 | 5.70E-54 | -1.69E-46 |
| MS.gene049587 | MS.gene69594 | 0.816 | 6.93E-52 | -1.69E-46 |
| MS.gene049587 | MS.gene72205 | 0.807009 | 6.23E-50 | -1.69E-46 |
| MS.gene049587 | MS.gene74025 | 0.808189 | 3.50E-50 | -1.69E-46 |
| MS.gene049587 | MS.gene78647 | 0.815138 | 1.08E-51 | -1.69E-46 |
| MS.gene049587 | MS.gene81140 | 0.80114 | 1.04E-48 | -1.69E-46 |
| MS.gene049587 | MS.gene82668 | 0.817483 | 3.22E-52 | -1.69E-46 |
| MS.gene049587 | MS.gene84001 | 0.802995 | 4.30E-49 | -1.69E-46 |
| MS.gene049587 | MS.gene84968 | 0.803218 | 3.87E-49 | -1.69E-46 |
| MS.gene049587 | MS.gene91451 | 0.813355 | 2.67E-51 | -1.69E-46 |
| MS.gene049587 | MS.gene98673 | 0.800436 | 1.44E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049587.t1 | MTR_1g069325 | 94.000 | 50 | 3 | 0 | 3 | 52 | 65 | 114 | 1.00e-26 | 98.2 |
| MS.gene049587.t1 | MTR_1g069325 | 94.000 | 50 | 3 | 0 | 3 | 52 | 116 | 165 | 1.83e-26 | 98.6 |
| MS.gene049587.t1 | MTR_7g099270 | 53.571 | 56 | 26 | 0 | 3 | 58 | 112 | 167 | 5.76e-14 | 64.3 |
| MS.gene049587.t1 | MTR_7g099280 | 48.148 | 54 | 28 | 0 | 3 | 56 | 167 | 220 | 4.88e-12 | 58.9 |
| MS.gene049587.t1 | MTR_7g099280 | 52.000 | 50 | 24 | 0 | 3 | 52 | 157 | 206 | 9.14e-12 | 58.2 |
| MS.gene049587.t1 | MTR_7g099280 | 52.000 | 50 | 24 | 0 | 3 | 52 | 159 | 208 | 9.23e-12 | 58.2 |
| MS.gene049587.t1 | MTR_7g099280 | 52.000 | 50 | 24 | 0 | 3 | 52 | 157 | 206 | 1.91e-11 | 57.0 |
| MS.gene049587.t1 | MTR_7g099280 | 52.000 | 50 | 24 | 0 | 3 | 52 | 157 | 206 | 2.18e-11 | 57.0 |
| MS.gene049587.t1 | MTR_7g099280 | 52.000 | 50 | 24 | 0 | 3 | 52 | 157 | 206 | 2.33e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049587.t1 | AT4G28706 | 57.143 | 56 | 24 | 0 | 3 | 58 | 149 | 204 | 1.66e-15 | 68.6 |
| MS.gene049587.t1 | AT4G28706 | 57.143 | 56 | 24 | 0 | 3 | 58 | 151 | 206 | 1.70e-15 | 68.6 |
| MS.gene049587.t1 | AT4G28706 | 57.143 | 56 | 24 | 0 | 3 | 58 | 151 | 206 | 1.70e-15 | 68.6 |
| MS.gene049587.t1 | AT4G28706 | 57.143 | 56 | 24 | 0 | 3 | 58 | 151 | 206 | 1.77e-15 | 68.6 |
Find 9 sgRNAs with CRISPR-Local
Find 99 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGCATCCTAACATCAAAAT+AGG | 0.360561 | 3.2:+58498384 | None:intergenic |
| TTTGCACGCGAAAGATCTTC+TGG | 0.366371 | 3.2:+58498439 | None:intergenic |
| AGATCTTCTGGTACCATTGT+AGG | 0.407556 | 3.2:+58498451 | None:intergenic |
| GCAATGACAAGAGCAGTTGC+AGG | 0.440063 | 3.2:+58498364 | None:intergenic |
| TGCTCTTGTCATTGCTAAAG+AGG | 0.464049 | 3.2:-58498356 | MS.gene049587:intron |
| AAATTGTTGTCTGCATTGAA+TGG | 0.483068 | 3.2:-58498418 | MS.gene049587:CDS |
| TGCATTGAATGGAGCTAGAG+TGG | 0.576733 | 3.2:-58498407 | MS.gene049587:CDS |
| CACAGAAGGATCTCCTACAA+TGG | 0.585278 | 3.2:-58498464 | MS.gene049587:CDS |
| CGAACTTGTATATTCACAGA+AGG | 0.625943 | 3.2:-58498478 | MS.gene049587:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTAAGAATTTGTAAAAAT+AGG | + | chr3.2:58498236-58498255 | None:intergenic | 10.0% |
| !!! | ATTTTTACATTTATTCAATT+TGG | - | chr3.2:58498110-58498129 | MS.gene049587:intron | 10.0% |
| !!! | TTTTTACATTTATTCAATTT+GGG | - | chr3.2:58498111-58498130 | MS.gene049587:intron | 10.0% |
| !! | ATCATAATTCTAAATAGATT+CGG | - | chr3.2:58499165-58499184 | MS.gene049587:intron | 15.0% |
| !! | GTAGTACTTATAAATTTATT+TGG | + | chr3.2:58498340-58498359 | None:intergenic | 15.0% |
| !! | TAAATTAGTATTAGTTAGAA+TGG | - | chr3.2:58498251-58498270 | MS.gene049587:intron | 15.0% |
| !!! | TTTAACTTGATCAAAATTTT+TGG | + | chr3.2:58498712-58498731 | None:intergenic | 15.0% |
| !!! | TTTGTACTTTTATCTTTTTA+TGG | - | chr3.2:58499336-58499355 | MS.gene049587:intron | 15.0% |
| !! | ATGTAAAATCATTTAAGCAA+TGG | + | chr3.2:58498582-58498601 | None:intergenic | 20.0% |
| !! | CAGGACATATATAATATTAT+AGG | + | chr3.2:58497766-58497785 | None:intergenic | 20.0% |
| !! | CTGTTATTAATGAAAAATGA+TGG | + | chr3.2:58499430-58499449 | None:intergenic | 20.0% |
| !! | TACAAGTATATGAAAGTTAT+TGG | - | chr3.2:58498675-58498694 | MS.gene049587:intron | 20.0% |
| !! | TATACATGATAAAAATTGTC+AGG | + | chr3.2:58497785-58497804 | None:intergenic | 20.0% |
| !! | TCAATAATAATGTAGCTAAA+TGG | + | chr3.2:58498543-58498562 | None:intergenic | 20.0% |
| !!! | TTTTTCATTAATAACAGAGA+AGG | - | chr3.2:58499433-58499452 | MS.gene049587:intron | 20.0% |
| ! | AAAATAAAGATGAAGATGAG+GGG | + | chr3.2:58499319-58499338 | None:intergenic | 25.0% |
| ! | AAATAGCAAGTTTATGATGT+TGG | + | chr3.2:58498378-58498397 | None:intergenic | 25.0% |
| ! | ACAAAATAAAGATGAAGATG+AGG | + | chr3.2:58499321-58499340 | None:intergenic | 25.0% |
| ! | ATCGTTATACTTTAAGTCAT+TGG | - | chr3.2:58498195-58498214 | MS.gene049587:intron | 25.0% |
| ! | CAAAATAAAGATGAAGATGA+GGG | + | chr3.2:58499320-58499339 | None:intergenic | 25.0% |
| ! | GTTGAAATATGCTTAAACAA+TGG | + | chr3.2:58499635-58499654 | None:intergenic | 25.0% |
| ! | GTTGTAGCATAATGTAATAT+AGG | - | chr3.2:58499139-58499158 | MS.gene049587:intron | 25.0% |
| ! | TCAAGTTAAATTTAAGCACT+AGG | - | chr3.2:58498722-58498741 | MS.gene049587:intron | 25.0% |
| ! | TCTAAGTAAATTGTCATTGT+TGG | - | chr3.2:58499236-58499255 | MS.gene049587:intron | 25.0% |
| ! | TTTCCTTAGAGAAAACTAAT+TGG | + | chr3.2:58499539-58499558 | None:intergenic | 25.0% |
| !! | AATGAATTCTGAAGATTGTA+AGG | - | chr3.2:58499359-58499378 | MS.gene049587:intron | 25.0% |
| !! | ATTGATGATATGCTCTTAAT+AGG | - | chr3.2:58499481-58499500 | MS.gene049587:intron | 25.0% |
| !! | ATTTGGTAGTTCTACTAAAT+TGG | + | chr3.2:58498323-58498342 | None:intergenic | 25.0% |
| !! | TTTATGACTCTTGAAACTTT+TGG | - | chr3.2:58498080-58498099 | MS.gene049587:intron | 25.0% |
| AAATAAAGATGAAGATGAGG+GGG | + | chr3.2:58499318-58499337 | None:intergenic | 30.0% | |
| AAATGCTGAATAGTATGGTA+AGG | - | chr3.2:58499557-58499576 | MS.gene049587:intron | 30.0% | |
| AAATTGTTGTCTGCATTGAA+TGG | - | chr3.2:58499041-58499060 | MS.gene049587:intron | 30.0% | |
| AAGGAAAATGCTGAATAGTA+TGG | - | chr3.2:58499552-58499571 | MS.gene049587:intron | 30.0% | |
| AAGTATAACGATATCATGAG+TGG | + | chr3.2:58498187-58498206 | None:intergenic | 30.0% | |
| AATTGGATTTCTTAAGCATG+TGG | - | chr3.2:58498808-58498827 | MS.gene049587:intron | 30.0% | |
| AGACATGCTTGATAGATAAT+TGG | - | chr3.2:58498791-58498810 | MS.gene049587:intron | 30.0% | |
| CACTTCTATACCTATTATGT+GGG | - | chr3.2:58499673-58499692 | MS.gene049587:intron | 30.0% | |
| CTAAAAATCACCAAACAGTA+TGG | + | chr3.2:58497975-58497994 | None:intergenic | 30.0% | |
| GATTTGCATTGAAAGAGATA+TGG | - | chr3.2:58498285-58498304 | MS.gene049587:intron | 30.0% | |
| TATGAAGTAGCATCTATCTA+AGG | + | chr3.2:58498141-58498160 | None:intergenic | 30.0% | |
| TGTATATCTTGCTTCACAAT+TGG | - | chr3.2:58497799-58497818 | MS.gene049587:intron | 30.0% | |
| ! | CTATTAGTACTTTTCTCAAG+AGG | - | chr3.2:58499211-58499230 | MS.gene049587:intron | 30.0% |
| ! | GAAAATTTGAGATGAACACT+AGG | - | chr3.2:58498025-58498044 | MS.gene049587:intron | 30.0% |
| ! | GTTCCCAAATTAGTACTTTT+TGG | - | chr3.2:58499273-58499292 | MS.gene049587:intron | 30.0% |
| ! | TAAGCATGTGGTTTGATAAT+CGG | - | chr3.2:58498820-58498839 | MS.gene049587:intron | 30.0% |
| ! | TAGATAGATGCTACTTCATA+AGG | - | chr3.2:58498141-58498160 | MS.gene049587:intron | 30.0% |
| !! | AAGCACTAGGAGTATTAAAA+AGG | - | chr3.2:58498735-58498754 | MS.gene049587:intron | 30.0% |
| !! | ATAATCAACTTTTGCCACTT+CGG | + | chr3.2:58499599-58499618 | None:intergenic | 30.0% |
| !! | CCAAATTAGTACTTTTTGGA+GGG | - | chr3.2:58499277-58499296 | MS.gene049587:intron | 30.0% |
| !! | CCTCCAAAAAGTACTAATTT+GGG | + | chr3.2:58499279-58499298 | None:intergenic | 30.0% |
| !! | TAACTGCTAATTTTGACACA+TGG | - | chr3.2:58498888-58498907 | MS.gene049587:intron | 30.0% |
| !!! | TCTCCAATTAGTTTTCTCTA+AGG | - | chr3.2:58499533-58499552 | MS.gene049587:intron | 30.0% |
| AATAAAGATGAAGATGAGGG+GGG | + | chr3.2:58499317-58499336 | None:intergenic | 35.0% | |
| AGGCATCCTAACATCAAAAT+AGG | + | chr3.2:58499078-58499097 | None:intergenic | 35.0% | |
| CAAGTGAAAAATTAGGAGGA+AGG | + | chr3.2:58498624-58498643 | None:intergenic | 35.0% | |
| CGAACTTGTATATTCACAGA+AGG | - | chr3.2:58498981-58499000 | MS.gene049587:intron | 35.0% | |
| GAACCTGTTTGAAAACAAAC+TGG | + | chr3.2:58499724-58499743 | None:intergenic | 35.0% | |
| GACAGTAGACACTATGTTAT+TGG | + | chr3.2:58498766-58498785 | None:intergenic | 35.0% | |
| GCACTTCTATACCTATTATG+TGG | - | chr3.2:58499672-58499691 | MS.gene049587:intron | 35.0% | |
| TACTTCATAAGGCAATAGCA+TGG | - | chr3.2:58498152-58498171 | MS.gene049587:intron | 35.0% | |
| TCCTATAACGTTGAACATTC+AGG | - | chr3.2:58498952-58498971 | MS.gene049587:intron | 35.0% | |
| TCCTGAATGTTCAACGTTAT+AGG | + | chr3.2:58498956-58498975 | None:intergenic | 35.0% | |
| TTCCTTTCTCATTTGTTTCC+AGG | - | chr3.2:58498497-58498516 | MS.gene049587:intron | 35.0% | |
| ! | CCTCCTAATTTTTCACTTGT+GGG | - | chr3.2:58498625-58498644 | MS.gene049587:intron | 35.0% |
| ! | CTCCTAATTTTTCACTTGTG+GGG | - | chr3.2:58498626-58498645 | MS.gene049587:intron | 35.0% |
| ! | TCCTCCTAATTTTTCACTTG+TGG | - | chr3.2:58498624-58498643 | MS.gene049587:intron | 35.0% |
| ! | TCTCTCTCTCTGATTTTGTT+AGG | - | chr3.2:58497929-58497948 | MS.gene049587:intron | 35.0% |
| !! | CCCAAATTAGTACTTTTTGG+AGG | - | chr3.2:58499276-58499295 | MS.gene049587:intron | 35.0% |
| !! | CCCTCCAAAAAGTACTAATT+TGG | + | chr3.2:58499280-58499299 | None:intergenic | 35.0% |
| !! | TAATCAACTTTTGCCACTTC+GGG | + | chr3.2:58499598-58499617 | None:intergenic | 35.0% |
| !! | TAGTACTTTTTGGAGGGTTT+TGG | - | chr3.2:58499283-58499302 | MS.gene049587:intron | 35.0% |
| !! | TCTCCAGTTTGTTTTCAAAC+AGG | - | chr3.2:58499718-58499737 | MS.gene049587:intron | 35.0% |
| !! | TGGAGAACACTTTTTAACCA+GGG | + | chr3.2:58499704-58499723 | None:intergenic | 35.0% |
| AACGATATCATGAGTGGTGT+TGG | + | chr3.2:58498181-58498200 | None:intergenic | 40.0% | |
| AGATCTTCTGGTACCATTGT+AGG | + | chr3.2:58499011-58499030 | None:intergenic | 40.0% | |
| AGCCCCACAAGTGAAAAATT+AGG | + | chr3.2:58498631-58498650 | None:intergenic | 40.0% | |
| CATTTGTTTCCAGGTCTCAA+AGG | - | chr3.2:58498506-58498525 | MS.gene049587:intron | 40.0% | |
| CCCACAAGTGAAAAATTAGG+AGG | + | chr3.2:58498628-58498647 | None:intergenic | 40.0% | |
| GACCTGGAAACAAATGAGAA+AGG | + | chr3.2:58498502-58498521 | None:intergenic | 40.0% | |
| GCATTGAAAGAGATATGGCA+GGG | - | chr3.2:58498290-58498309 | MS.gene049587:intron | 40.0% | |
| GTTCTATGAGCCATACTGTT+TGG | - | chr3.2:58497962-58497981 | MS.gene049587:intron | 40.0% | |
| TAAGCAATGGTACATACGTG+TGG | + | chr3.2:58498569-58498588 | None:intergenic | 40.0% | |
| TGCATTGAAAGAGATATGGC+AGG | - | chr3.2:58498289-58498308 | MS.gene049587:intron | 40.0% | |
| TGCTCTTGTCATTGCTAAAG+AGG | - | chr3.2:58499103-58499122 | MS.gene049587:intron | 40.0% | |
| ! | CTCTCTCTGATTTTGTTAGG+TGG | - | chr3.2:58497932-58497951 | MS.gene049587:intron | 40.0% |
| ! | TCTGATTTTGTTAGGTGGAG+CGG | - | chr3.2:58497937-58497956 | MS.gene049587:intron | 40.0% |
| !! | CTGGAGAACACTTTTTAACC+AGG | + | chr3.2:58499705-58499724 | None:intergenic | 40.0% |
| !!! | GAGTGGCCTATTTTGATGTT+AGG | - | chr3.2:58499069-58499088 | MS.gene049587:intron | 40.0% |
| CACAGAAGGATCTCCTACAA+TGG | - | chr3.2:58498995-58499014 | MS.gene049587:intron | 45.0% | |
| GTTTCCAGGTCTCAAAGGAA+GGG | - | chr3.2:58498511-58498530 | MS.gene049587:intron | 45.0% | |
| TCCAACGAAATTTCCCGAAG+TGG | - | chr3.2:58499582-58499601 | MS.gene049587:intron | 45.0% | |
| TGCATTGAATGGAGCTAGAG+TGG | - | chr3.2:58499052-58499071 | MS.gene049587:intron | 45.0% | |
| TGTTTCCAGGTCTCAAAGGA+AGG | - | chr3.2:58498510-58498529 | MS.gene049587:intron | 45.0% | |
| TTTGCACGCGAAAGATCTTC+TGG | + | chr3.2:58499023-58499042 | None:intergenic | 45.0% | |
| CAGGGAGAAGCCCACATAAT+AGG | + | chr3.2:58499686-58499705 | None:intergenic | 50.0% | |
| CTATTATGTGGGCTTCTCCC+TGG | - | chr3.2:58499684-58499703 | MS.gene049587:intron | 50.0% | |
| GCAATGACAAGAGCAGTTGC+AGG | + | chr3.2:58499098-58499117 | None:intergenic | 50.0% | |
| GCCACTTCGGGAAATTTCGT+TGG | + | chr3.2:58499586-58499605 | None:intergenic | 50.0% | |
| !! | TAGTCCCTTCCTTTGAGACC+TGG | + | chr3.2:58498518-58498537 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 58497715 | 58499766 | 58497715 | ID=MS.gene049587 |
| chr3.2 | mRNA | 58497715 | 58499766 | 58497715 | ID=MS.gene049587.t1;Parent=MS.gene049587 |
| chr3.2 | exon | 58499762 | 58499766 | 58499762 | ID=MS.gene049587.t1.exon1;Parent=MS.gene049587.t1 |
| chr3.2 | CDS | 58499762 | 58499766 | 58499762 | ID=cds.MS.gene049587.t1;Parent=MS.gene049587.t1 |
| chr3.2 | exon | 58498357 | 58498507 | 58498357 | ID=MS.gene049587.t1.exon2;Parent=MS.gene049587.t1 |
| chr3.2 | CDS | 58498357 | 58498507 | 58498357 | ID=cds.MS.gene049587.t1;Parent=MS.gene049587.t1 |
| chr3.2 | exon | 58497715 | 58497741 | 58497715 | ID=MS.gene049587.t1.exon3;Parent=MS.gene049587.t1 |
| chr3.2 | CDS | 58497715 | 58497741 | 58497715 | ID=cds.MS.gene049587.t1;Parent=MS.gene049587.t1 |
| Gene Sequence |
| Protein sequence |
