Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22776.t1 | XP_003626145.1 | 97.8 | 455 | 8 | 2 | 1 | 453 | 1 | 455 | 1.30E-261 | 911.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22776.t1 | Q3E6S9 | 71.9 | 430 | 121 | 0 | 23 | 452 | 45 | 474 | 2.7e-191 | 669.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22776.t1 | G7KWF1 | 97.8 | 455 | 8 | 2 | 1 | 453 | 1 | 455 | 9.4e-262 | 911.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050796 | MS.gene22776 | 0.804071 | 2.58E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene20906 | MS.gene22776 | PPI |
| MS.gene07363 | MS.gene22776 | PPI |
| MS.gene20907 | MS.gene22776 | PPI |
| MS.gene005429 | MS.gene22776 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22776.t1 | MTR_7g111540 | 97.802 | 455 | 8 | 2 | 1 | 453 | 1 | 455 | 0.0 | 928 |
| MS.gene22776.t1 | MTR_1g484920 | 71.018 | 452 | 116 | 7 | 4 | 452 | 5 | 444 | 0.0 | 668 |
| MS.gene22776.t1 | MTR_1g484920 | 63.778 | 450 | 109 | 7 | 4 | 452 | 5 | 401 | 0.0 | 582 |
| MS.gene22776.t1 | MTR_1g086070 | 60.000 | 445 | 167 | 4 | 16 | 453 | 9 | 449 | 0.0 | 559 |
| MS.gene22776.t1 | MTR_3g083980 | 24.291 | 247 | 157 | 9 | 52 | 286 | 79 | 307 | 1.31e-11 | 66.2 |
| MS.gene22776.t1 | MTR_3g083980 | 24.291 | 247 | 157 | 9 | 52 | 286 | 79 | 307 | 2.35e-11 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22776.t1 | AT5G26600 | 71.860 | 430 | 121 | 0 | 23 | 452 | 45 | 474 | 0.0 | 660 |
| MS.gene22776.t1 | AT5G26600 | 71.860 | 430 | 121 | 0 | 23 | 452 | 45 | 474 | 0.0 | 660 |
| MS.gene22776.t1 | AT3G62130 | 59.688 | 449 | 170 | 3 | 10 | 451 | 7 | 451 | 0.0 | 585 |
| MS.gene22776.t1 | AT3G62130 | 59.688 | 449 | 170 | 3 | 10 | 451 | 7 | 451 | 0.0 | 585 |
Find 0 sgRNAs with CRISPR-Local
Find 141 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATTGGAAGAAGATAGTTT+AGG | + | 50591:5628-5647 | None:intergenic | 25.0% |
| ! | ACTAAAAAGAATCCTAAAAC+TGG | - | 50591:6375-6394 | MS.gene22776:CDS | 25.0% |
| ! | TTCTTATCAGGTTTACAATA+AGG | - | 50591:6812-6831 | MS.gene22776:CDS | 25.0% |
| !! | ATACTAGTAATTTGCATAAG+TGG | - | 50591:6319-6338 | MS.gene22776:CDS | 25.0% |
| !!! | AAGATAGTTTAGGTTTTTTG+GGG | + | 50591:5618-5637 | None:intergenic | 25.0% |
| !!! | AGAAGATAGTTTAGGTTTTT+TGG | + | 50591:5620-5639 | None:intergenic | 25.0% |
| !!! | GAAGATAGTTTAGGTTTTTT+GGG | + | 50591:5619-5638 | None:intergenic | 25.0% |
| !!! | TTAGAAAAGCTTTGGAAAAA+GGG | - | 50591:6109-6128 | MS.gene22776:CDS | 25.0% |
| !!! | TTTAGAAAAGCTTTGGAAAA+AGG | - | 50591:6108-6127 | MS.gene22776:CDS | 25.0% |
| AAAAAGAATCCTAAAACTGG+TGG | - | 50591:6378-6397 | MS.gene22776:CDS | 30.0% | |
| ATACTAGCACACATATGTTT+CGG | + | 50591:6633-6652 | None:intergenic | 30.0% | |
| CTTTGTTGAATTTACCTTCA+CGG | + | 50591:5948-5967 | None:intergenic | 30.0% | |
| GACTAAGTTTAGAAAAGCTT+TGG | - | 50591:6101-6120 | MS.gene22776:CDS | 30.0% | |
| GATGTTGATATGCAAGATAT+TGG | - | 50591:6285-6304 | MS.gene22776:CDS | 30.0% | |
| GTTATGCTTCATTATGCTTA+CGG | - | 50591:5979-5998 | MS.gene22776:CDS | 30.0% | |
| TGGGAAGAAAATTAGATTAG+CGG | - | 50591:6140-6159 | MS.gene22776:CDS | 30.0% | |
| TTGAGGACTATTACAAGTTT+CGG | - | 50591:6835-6854 | MS.gene22776:CDS | 30.0% | |
| ! | GATTTTGTGAATAGGTTTGA+AGG | - | 50591:6516-6535 | MS.gene22776:CDS | 30.0% |
| !!! | AGATAGTTTAGGTTTTTTGG+GGG | + | 50591:5617-5636 | None:intergenic | 30.0% |
| AAGGGATTATAGTGCTCAAT+TGG | - | 50591:6476-6495 | MS.gene22776:CDS | 35.0% | |
| ATCTGAATCAATTCCTTCAC+AGG | + | 50591:6204-6223 | None:intergenic | 35.0% | |
| CATGAAGCTGTTATTGAGAT+GGG | - | 50591:6567-6586 | MS.gene22776:CDS | 35.0% | |
| CGTGAAGGTAAATTCAACAA+AGG | - | 50591:5946-5965 | MS.gene22776:CDS | 35.0% | |
| CTAGGCGGTCTATAATATAT+AGG | + | 50591:6753-6772 | None:intergenic | 35.0% | |
| GAAACATATGTGTGCTAGTA+TGG | - | 50591:6632-6651 | MS.gene22776:CDS | 35.0% | |
| GTATGCAAGGATTTCTTATC+AGG | - | 50591:6800-6819 | MS.gene22776:CDS | 35.0% | |
| TATGTGTGCTAGTATGGTTA+TGG | - | 50591:6638-6657 | MS.gene22776:CDS | 35.0% | |
| TCAGGTTTACAATAAGGTTG+AGG | - | 50591:6818-6837 | MS.gene22776:CDS | 35.0% | |
| TCATGAAGCTGTTATTGAGA+TGG | - | 50591:6566-6585 | MS.gene22776:CDS | 35.0% | |
| TGAGGACTATTACAAGTTTC+GGG | - | 50591:6836-6855 | MS.gene22776:CDS | 35.0% | |
| TTGGAAAAAGGGAAAGTTGA+TGG | - | 50591:6120-6139 | MS.gene22776:CDS | 35.0% | |
| TTGTGAATAGGTTTGAAGGA+GGG | - | 50591:6520-6539 | MS.gene22776:CDS | 35.0% | |
| TTTGTGAATAGGTTTGAAGG+AGG | - | 50591:6519-6538 | MS.gene22776:CDS | 35.0% | |
| ! | ACACATTTGAGGGATGTTTT+TGG | - | 50591:6717-6736 | MS.gene22776:CDS | 35.0% |
| ! | ATTGATTCAGATTTGCAGAG+AGG | - | 50591:6212-6231 | MS.gene22776:CDS | 35.0% |
| ! | TGGAAAAAGGGAAAGTTGAT+GGG | - | 50591:6121-6140 | MS.gene22776:CDS | 35.0% |
| ! | TTAACCAACTGGTGGATAAT+GGG | - | 50591:6865-6884 | MS.gene22776:CDS | 35.0% |
| ! | TTCGGATTCAATTTCTGAAG+CGG | + | 50591:5656-5675 | None:intergenic | 35.0% |
| !!! | AGGCTTTGGATTTTGTGAAT+AGG | - | 50591:6508-6527 | MS.gene22776:CDS | 35.0% |
| !!! | CTAGGTTTTGGATAATTGAG+CGG | + | 50591:5837-5856 | None:intergenic | 35.0% |
| !!! | TAGGTTTTGGATAATTGAGC+GGG | + | 50591:5836-5855 | None:intergenic | 35.0% |
| !!! | TTGGTTTGCCTACTTGTTTT+GGG | - | 50591:6661-6680 | MS.gene22776:CDS | 35.0% |
| AAGAATCCTAAAACTGGTGG+TGG | - | 50591:6381-6400 | MS.gene22776:CDS | 40.0% | |
| ATTCAGATTTGCAGAGAGGA+AGG | - | 50591:6216-6235 | MS.gene22776:CDS | 40.0% | |
| ATTCTGATGCACTTAAGCTG+AGG | - | 50591:6694-6713 | MS.gene22776:CDS | 40.0% | |
| CTTCTAACCCCAAAACAAGT+AGG | + | 50591:6672-6691 | None:intergenic | 40.0% | |
| GGATTATAGTGCTCAATTGG+TGG | - | 50591:6479-6498 | MS.gene22776:CDS | 40.0% | |
| GGATTGAAGGGATTAAGAAG+AGG | - | 50591:6541-6560 | MS.gene22776:CDS | 40.0% | |
| GTATACAAAAACGCGATCGA+AGG | + | 50591:6357-6376 | None:intergenic | 40.0% | |
| GTGGTTGAAGTAGAAGTGAT+CGG | + | 50591:5791-5810 | None:intergenic | 40.0% | |
| GTGTCTCATGAGTATGGAAA+TGG | - | 50591:6423-6442 | MS.gene22776:CDS | 40.0% | |
| TAAGCTGAGGACACATTTGA+GGG | - | 50591:6707-6726 | MS.gene22776:CDS | 40.0% | |
| TATTATAGACCGCCTAGAGA+TGG | - | 50591:6756-6775 | MS.gene22776:CDS | 40.0% | |
| TATTGAGATGGGAGAGATGT+TGG | - | 50591:6578-6597 | MS.gene22776:CDS | 40.0% | |
| TGCTGTTAAGAAATCCATGG+AGG | - | 50591:6002-6021 | MS.gene22776:CDS | 40.0% | |
| TGGTTGAAGTAGAAGTGATC+GGG | + | 50591:5790-5809 | None:intergenic | 40.0% | |
| TGTGCTAGTATGGTTATGGT+TGG | - | 50591:6642-6661 | MS.gene22776:CDS | 40.0% | |
| TTAAGCTGAGGACACATTTG+AGG | - | 50591:6706-6725 | MS.gene22776:CDS | 40.0% | |
| TTCACAAAATCCAAAGCCTC+AGG | + | 50591:6507-6526 | None:intergenic | 40.0% | |
| TTCACAGGAATCACAACACA+AGG | + | 50591:6189-6208 | None:intergenic | 40.0% | |
| TTGTGTTGTGATTCCTGTGA+AGG | - | 50591:6188-6207 | MS.gene22776:CDS | 40.0% | |
| ! | AAATCACCACCACCAGTTTT+AGG | + | 50591:6390-6409 | None:intergenic | 40.0% |
| ! | AGAGATGTTGGTGAAAGCTT+GGG | - | 50591:6590-6609 | MS.gene22776:CDS | 40.0% |
| ! | GTTAACCAACTGGTGGATAA+TGG | - | 50591:6864-6883 | MS.gene22776:CDS | 40.0% |
| ! | TGAAGTTCCGTTACCTTTTC+CGG | - | 50591:6056-6075 | MS.gene22776:CDS | 40.0% |
| !! | GTATGGAAATGGATTAGCTG+TGG | - | 50591:6434-6453 | MS.gene22776:CDS | 40.0% |
| !! | GTGGTGATTGTTTTTCTCAG+AGG | + | 50591:5560-5579 | None:intergenic | 40.0% |
| !! | TGATGTCTTTAGCGTTGACT+AGG | + | 50591:5855-5874 | None:intergenic | 40.0% |
| !! | TTCTGAAGCGGTGATGAAAT+TGG | + | 50591:5644-5663 | None:intergenic | 40.0% |
| !!! | AGTTTAGGTTTTTTGGGGGT+GGG | + | 50591:5613-5632 | None:intergenic | 40.0% |
| !!! | CTTTAGCGTTGACTAGGTTT+TGG | + | 50591:5849-5868 | None:intergenic | 40.0% |
| !!! | GTTGGTTTGCCTACTTGTTT+TGG | - | 50591:6660-6679 | MS.gene22776:CDS | 40.0% |
| !!! | TAGTTTAGGTTTTTTGGGGG+TGG | + | 50591:5614-5633 | None:intergenic | 40.0% |
| !!! | TGGTTTGCCTACTTGTTTTG+GGG | - | 50591:6662-6681 | MS.gene22776:CDS | 40.0% |
| AAAGCTTGGGGAACTCATCT+TGG | - | 50591:6603-6622 | MS.gene22776:CDS | 45.0% | |
| AAGCTTGGGGAACTCATCTT+GGG | - | 50591:6604-6623 | MS.gene22776:CDS | 45.0% | |
| AATTGAGCGGGAGTTGTTGA+TGG | + | 50591:5824-5843 | None:intergenic | 45.0% | |
| AGGTTTGAAGGAGGGATTGA+AGG | - | 50591:6528-6547 | MS.gene22776:CDS | 45.0% | |
| ATCTCATCGTTCGAACTCAC+CGG | + | 50591:6078-6097 | None:intergenic | 45.0% | |
| CAAACCCATTATCCACCAGT+TGG | + | 50591:6872-6891 | None:intergenic | 45.0% | |
| CCATACTCATGAGACACAAC+AGG | + | 50591:6420-6439 | None:intergenic | 45.0% | |
| CCTGTTGTGTCTCATGAGTA+TGG | - | 50591:6417-6436 | MS.gene22776:CDS | 45.0% | |
| CGTTCAATCCCAGACATCAA+CGG | - | 50591:5580-5599 | MS.gene22776:CDS | 45.0% | |
| GAACTCACCGGAAAAGGTAA+CGG | + | 50591:6066-6085 | None:intergenic | 45.0% | |
| GGATCAACTTCTCCATCTCT+AGG | + | 50591:6771-6790 | None:intergenic | 45.0% | |
| GGTGTGTTGAAGAACGATAG+CGG | + | 50591:5914-5933 | None:intergenic | 45.0% | |
| GGTTTGAAGGAGGGATTGAA+GGG | - | 50591:6529-6548 | MS.gene22776:CDS | 45.0% | |
| GTTGATGCTGCTCATGCAAT+TGG | - | 50591:6255-6274 | MS.gene22776:CDS | 45.0% | |
| TACAAAAACGCGATCGAAGG+TGG | + | 50591:6354-6373 | None:intergenic | 45.0% | |
| TCAACTTCTCCATCTCTAGG+CGG | + | 50591:6768-6787 | None:intergenic | 45.0% | |
| ! | CGTTGATGTCTGGGATTGAA+CGG | + | 50591:5582-5601 | None:intergenic | 45.0% |
| ! | GAGAGATGTTGGTGAAAGCT+TGG | - | 50591:6589-6608 | MS.gene22776:CDS | 45.0% |
| ! | GAGATGTTGGTGAAAGCTTG+GGG | - | 50591:6591-6610 | MS.gene22776:CDS | 45.0% |
| ! | TGGAGAAGTTGATCCTGTGA+CGG | - | 50591:6776-6795 | MS.gene22776:CDS | 45.0% |
| !! | AAAGTGCTTGGATTGGCACA+AGG | - | 50591:6457-6476 | MS.gene22776:CDS | 45.0% |
| !! | AAGTGCTTGGATTGGCACAA+GGG | - | 50591:6458-6477 | MS.gene22776:CDS | 45.0% |
| !! | CGGTGCTGTTAAGAAATCCA+TGG | - | 50591:5999-6018 | MS.gene22776:CDS | 45.0% |
| !! | GATTAGCTGTGGAAAGTGCT+TGG | - | 50591:6445-6464 | MS.gene22776:CDS | 45.0% |
| !!! | GTTTAGGTTTTTTGGGGGTG+GGG | + | 50591:5612-5631 | None:intergenic | 45.0% |
| ACCTTCACGGAAACACCAAG+CGG | + | 50591:5935-5954 | None:intergenic | 50.0% | |
| CGCATTAACAACGGCAGCTT+TGG | - | 50591:5703-5722 | MS.gene22776:CDS | 50.0% | |
| GGATGCTGTTAACCAACTGG+TGG | - | 50591:6857-6876 | MS.gene22776:CDS | 50.0% | |
| TCCTCTCCGCTCAACAACAA+TGG | - | 50591:5743-5762 | MS.gene22776:CDS | 50.0% | |
| TCGGGATGCTGTTAACCAAC+TGG | - | 50591:6854-6873 | MS.gene22776:CDS | 50.0% | |
| TCGTTCGAACTCACCGGAAA+AGG | + | 50591:6072-6091 | None:intergenic | 50.0% | |
| TCGTTCTTCAACACACCGCT+TGG | - | 50591:5917-5936 | MS.gene22776:CDS | 50.0% | |
| ! | ATTGGTGGTTCCTGAGGCTT+TGG | - | 50591:6494-6513 | MS.gene22776:CDS | 50.0% |
| ! | GAGAAGTTGATCCTGTGACG+GGG | - | 50591:6778-6797 | MS.gene22776:CDS | 50.0% |
| ! | GAGCTGCCATTGTTGTTGAG+CGG | + | 50591:5752-5771 | None:intergenic | 50.0% |
| ! | GGAGAAGTTGATCCTGTGAC+GGG | - | 50591:6777-6796 | MS.gene22776:CDS | 50.0% |
| ! | TGATGTCTGGGATTGAACGG+TGG | + | 50591:5579-5598 | None:intergenic | 50.0% |
| ! | TGCTCAATTGGTGGTTCCTG+AGG | - | 50591:6488-6507 | MS.gene22776:CDS | 50.0% |
| ! | TTGAAGAACGATAGCGGCAG+CGG | + | 50591:5908-5927 | None:intergenic | 50.0% |
| ! | TTGTTGTTGAGCGGAGAGGA+TGG | + | 50591:5743-5762 | None:intergenic | 50.0% |
| !! | AGTTGTTGATGGCGGGTTTG+AGG | + | 50591:5813-5832 | None:intergenic | 50.0% |
| !! | CAGAGAGGAAGGTGTTGAAC+AGG | - | 50591:6227-6246 | MS.gene22776:CDS | 50.0% |
| !! | GCTGTGGAAAGTGCTTGGAT+TGG | - | 50591:6450-6469 | MS.gene22776:CDS | 50.0% |
| ACGAGTGACGTAAGCCTCCA+TGG | + | 50591:6019-6038 | None:intergenic | 55.0% | |
| ATCCTTGCATACCCCGTCAC+AGG | + | 50591:6792-6811 | None:intergenic | 55.0% | |
| GCCGTCGCTCGCATTAACAA+CGG | - | 50591:5694-5713 | MS.gene22776:CDS | 55.0% | |
| GCCGTTGTTAATGCGAGCGA+CGG | + | 50591:5698-5717 | None:intergenic | 55.0% | |
| GTTGTTAATGCGAGCGACGG+CGG | + | 50591:5695-5714 | None:intergenic | 55.0% | |
| TTGTTGAGCGGAGAGGATGG+AGG | + | 50591:5740-5759 | None:intergenic | 55.0% | |
| ! | ATCCTGTGACGGGGTATGCA+AGG | - | 50591:6787-6806 | MS.gene22776:CDS | 55.0% |
| ! | CTTACGTCACTCGTGCTGGT+GGG | - | 50591:6025-6044 | MS.gene22776:CDS | 55.0% |
| ! | GCCATTGTTGTTGAGCGGAG+AGG | + | 50591:5747-5766 | None:intergenic | 55.0% |
| ! | GGTGTGTGCCGTTGATGTCT+GGG | + | 50591:5591-5610 | None:intergenic | 55.0% |
| ! | TGAGCGGGAGTTGTTGATGG+CGG | + | 50591:5821-5840 | None:intergenic | 55.0% |
| !! | ACCGCTTGGTGTTTCCGTGA+AGG | - | 50591:5931-5950 | MS.gene22776:CDS | 55.0% |
| !! | TGTTGATGGCGGGTTTGAGG+TGG | + | 50591:5810-5829 | None:intergenic | 55.0% |
| GAGGCTTACGTCACTCGTGC+TGG | - | 50591:6021-6040 | MS.gene22776:CDS | 60.0% | |
| GCTTACGTCACTCGTGCTGG+TGG | - | 50591:6024-6043 | MS.gene22776:CDS | 60.0% | |
| GGAGAGGATGGAGGAAGGAC+AGG | + | 50591:5731-5750 | None:intergenic | 60.0% | |
| GGCGTCGTGGTGGCTGAATT+CGG | + | 50591:5674-5693 | None:intergenic | 60.0% | |
| TGAGCGGAGAGGATGGAGGA+AGG | + | 50591:5736-5755 | None:intergenic | 60.0% | |
| ! | AAGAACGATAGCGGCAGCGG+TGG | + | 50591:5905-5924 | None:intergenic | 60.0% |
| ! | GGGTGTGTGCCGTTGATGTC+TGG | + | 50591:5592-5611 | None:intergenic | 60.0% |
| !! | GAGCGGGAGTTGTTGATGGC+GGG | + | 50591:5820-5839 | None:intergenic | 60.0% |
| ! | AACGATAGCGGCAGCGGTGG+TGG | + | 50591:5902-5921 | None:intergenic | 65.0% |
| ! | GAGCGACGGCGGCGTCGTGG+TGG | + | 50591:5684-5703 | None:intergenic | 80.0% |
| ! | TGCGAGCGACGGCGGCGTCG+TGG | + | 50591:5687-5706 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 50591 | gene | 5553 | 6914 | 5553 | ID=MS.gene22776 |
| 50591 | mRNA | 5553 | 6914 | 5553 | ID=MS.gene22776.t1;Parent=MS.gene22776 |
| 50591 | exon | 5553 | 6914 | 5553 | ID=MS.gene22776.t1.exon1;Parent=MS.gene22776.t1 |
| 50591 | CDS | 5553 | 6914 | 5553 | ID=cds.MS.gene22776.t1;Parent=MS.gene22776.t1 |
| Gene Sequence |
| Protein sequence |
