Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22891.t1 | XP_024625242.1 | 97.8 | 138 | 3 | 0 | 1 | 138 | 132 | 269 | 2.30E-68 | 268.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22891.t1 | A0A072U4H1 | 97.8 | 137 | 3 | 0 | 2 | 138 | 129 | 265 | 4.8e-68 | 266.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049758 | MS.gene22891 | 0.819472 | 1.14E-52 | -1.69E-46 |
| MS.gene050301 | MS.gene22891 | 0.824575 | 7.49E-54 | -1.69E-46 |
| MS.gene050558 | MS.gene22891 | 0.853934 | 1.72E-61 | -1.69E-46 |
| MS.gene051860 | MS.gene22891 | 0.81975 | 9.86E-53 | -1.69E-46 |
| MS.gene052207 | MS.gene22891 | 0.833397 | 5.46E-56 | -1.69E-46 |
| MS.gene053836 | MS.gene22891 | 0.822691 | 2.07E-53 | -1.69E-46 |
| MS.gene054473 | MS.gene22891 | 0.82828 | 9.81E-55 | -1.69E-46 |
| MS.gene055277 | MS.gene22891 | 0.804459 | 2.14E-49 | -1.69E-46 |
| MS.gene055422 | MS.gene22891 | 0.819798 | 9.61E-53 | -1.69E-46 |
| MS.gene057121 | MS.gene22891 | 0.839469 | 1.56E-57 | -1.69E-46 |
| MS.gene057245 | MS.gene22891 | 0.812245 | 4.67E-51 | -1.69E-46 |
| MS.gene058002 | MS.gene22891 | 0.804901 | 1.73E-49 | -1.69E-46 |
| MS.gene05811 | MS.gene22891 | 0.836536 | 8.83E-57 | -1.69E-46 |
| MS.gene059518 | MS.gene22891 | 0.825422 | 4.73E-54 | -1.69E-46 |
| MS.gene05984 | MS.gene22891 | 0.814319 | 1.64E-51 | -1.69E-46 |
| MS.gene06037 | MS.gene22891 | 0.807556 | 4.77E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22891.t1 | MTR_7g115670 | 97.810 | 137 | 3 | 0 | 2 | 138 | 129 | 265 | 2.26e-96 | 278 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22891.t1 | AT1G08640 | 68.613 | 137 | 43 | 0 | 2 | 138 | 138 | 274 | 3.62e-65 | 200 |
Find 27 sgRNAs with CRISPR-Local
Find 103 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGCCTTTGCTTTCGTTTAT+AGG | 0.144704 | 7.2:-1932103 | MS.gene22891:CDS |
| CTTGGTCCCCAAGGTATAAT+TGG | 0.268204 | 7.2:+1932781 | None:intergenic |
| ACTGCTCCGGTCGCTGGCCT+TGG | 0.308462 | 7.2:-1931867 | MS.gene22891:CDS |
| ATGTATAGCTGTTTCATCCT+TGG | 0.412262 | 7.2:-1931837 | MS.gene22891:intron |
| AAAGAGACTGCTCCGGTCGC+TGG | 0.428177 | 7.2:-1931873 | MS.gene22891:CDS |
| CCCATACGCAGTCCTTCTCC+TGG | 0.433111 | 7.2:+1931896 | None:intergenic |
| CAAACAGCCAATTATACCTT+GGG | 0.467405 | 7.2:-1932788 | MS.gene22891:CDS |
| CAGGAAGATGGTGAAGATCC+AGG | 0.474884 | 7.2:-1931914 | MS.gene22891:CDS |
| ATCTCTTCTTTACAACAGTC+AGG | 0.475809 | 7.2:-1930929 | None:intergenic |
| TCCAGGAGAAGGACTGCGTA+TGG | 0.496377 | 7.2:-1931897 | MS.gene22891:CDS |
| TTGTCTTTCTGCAGACAGCT+TGG | 0.506000 | 7.2:-1932169 | MS.gene22891:intron |
| TGACTGTTGTAAAGAAGAGA+TGG | 0.528342 | 7.2:+1930931 | None:intergenic |
| AAATTGAATGCAGGTTTCAA+AGG | 0.532979 | 7.2:-1932827 | None:intergenic |
| TATGAAACTCACATTAATTG+TGG | 0.549027 | 7.2:+1932040 | None:intergenic |
| CAATTATACCTTGGGGACCA+AGG | 0.553731 | 7.2:-1932780 | MS.gene22891:intron |
| ACAAACAGCCAATTATACCT+TGG | 0.553845 | 7.2:-1932789 | MS.gene22891:CDS |
| AACATAGTTGAGTCTGCAAG+TGG | 0.554259 | 7.2:-1930961 | MS.gene22891:CDS |
| AAATGATGTGCGCATCAACT+TGG | 0.557136 | 7.2:-1932270 | MS.gene22891:CDS |
| CCAGGAGAAGGACTGCGTAT+GGG | 0.593890 | 7.2:-1931896 | MS.gene22891:CDS |
| GTATGGGAAAGAGACTGCTC+CGG | 0.601329 | 7.2:-1931880 | MS.gene22891:CDS |
| GACTGTTGTAAAGAAGAGAT+GGG | 0.602985 | 7.2:+1930932 | None:intergenic |
| GATGGTGAAGATCCAGGAGA+AGG | 0.603751 | 7.2:-1931908 | MS.gene22891:CDS |
| ATAACAAACCTTGGTCCCCA+AGG | 0.616918 | 7.2:+1932772 | None:intergenic |
| AAGCCTATAAACGAAAGCAA+AGG | 0.649140 | 7.2:+1932100 | None:intergenic |
| TAATGTTGGATACAGGAAGA+TGG | 0.650849 | 7.2:-1931926 | MS.gene22891:intron |
| TCTGCAGACAGCTTGGATCG+CGG | 0.669493 | 7.2:-1932162 | MS.gene22891:intron |
| AAACAGCCAATTATACCTTG+GGG | 0.721557 | 7.2:-1932787 | MS.gene22891:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTAAATACAAATTACTCA+TGG | + | chr7.2:1931229-1931248 | None:intergenic | 15.0% |
| !!! | AATTTTAAAGAAGAAATTTG+TGG | - | chr7.2:1932411-1932430 | MS.gene22891:intron | 15.0% |
| !!! | AATTTTCATTTAATCTTTGT+TGG | - | chr7.2:1932370-1932389 | MS.gene22891:intron | 15.0% |
| !! | AGAAACTATTAATCTTTATC+TGG | + | chr7.2:1932734-1932753 | None:intergenic | 20.0% |
| !! | AGATAAAGATTAATAGTTTC+TGG | - | chr7.2:1932733-1932752 | MS.gene22891:intron | 20.0% |
| !! | ATAAGAAAATAGAAAACTTC+AGG | + | chr7.2:1932675-1932694 | None:intergenic | 20.0% |
| !! | GTAATCTACAATAATTAACA+AGG | - | chr7.2:1932007-1932026 | MS.gene22891:intron | 20.0% |
| !! | TAATTCAACATGGTTAAAAA+TGG | + | chr7.2:1931773-1931792 | None:intergenic | 20.0% |
| !! | TGTCATGATGAAAAATAATT+TGG | + | chr7.2:1932328-1932347 | None:intergenic | 20.0% |
| !! | TTAATTATTGTAGATTACAG+AGG | + | chr7.2:1932005-1932024 | None:intergenic | 20.0% |
| !!! | ATTCTAAGATTGTATTGAAT+AGG | + | chr7.2:1931747-1931766 | None:intergenic | 20.0% |
| !!! | ATTCTATTGATAATTCAACA+TGG | + | chr7.2:1931783-1931802 | None:intergenic | 20.0% |
| !!! | ATTTATTTTCATCTACAGTT+TGG | - | chr7.2:1931251-1931270 | MS.gene22891:intron | 20.0% |
| !!! | TGTCTTTTTAAAGAAAAAGA+TGG | - | chr7.2:1932062-1932081 | MS.gene22891:CDS | 20.0% |
| ! | AATAAGAAGTGAGAAAAACA+AGG | + | chr7.2:1932180-1932199 | None:intergenic | 25.0% |
| ! | AGCAGTAATAAAAGAGTAAT+TGG | + | chr7.2:1931165-1931184 | None:intergenic | 25.0% |
| ! | ATAATGTCAAAAAATCCAAG+TGG | + | chr7.2:1931420-1931439 | None:intergenic | 25.0% |
| ! | ATGGCATGTTTAATTCATAA+GGG | + | chr7.2:1931210-1931229 | None:intergenic | 25.0% |
| ! | ATGGTTGAATTGTGTTAATT+AGG | - | chr7.2:1931116-1931135 | MS.gene22891:intron | 25.0% |
| ! | TATGAAACTCACATTAATTG+TGG | + | chr7.2:1931713-1931732 | None:intergenic | 25.0% |
| ! | TGTCAAAATATCATTTCTCT+GGG | - | chr7.2:1932296-1932315 | MS.gene22891:intron | 25.0% |
| ! | TTCTTATTGGTCAAAAACAA+TGG | - | chr7.2:1932192-1932211 | MS.gene22891:intron | 25.0% |
| ! | TTGTCAAAATATCATTTCTC+TGG | - | chr7.2:1932295-1932314 | MS.gene22891:intron | 25.0% |
| ! | TTTAAAGAAGAAATTTGTGG+CGG | - | chr7.2:1932414-1932433 | MS.gene22891:intron | 25.0% |
| !! | TTCATGAATCATGATAATGT+TGG | - | chr7.2:1931810-1931829 | MS.gene22891:intron | 25.0% |
| !! | TTTTGTGAGTAGTAAATGAA+CGG | - | chr7.2:1931516-1931535 | MS.gene22891:intron | 25.0% |
| !!! | TTGTTTTTCTCACTTCTTAT+TGG | - | chr7.2:1932179-1932198 | MS.gene22891:intron | 25.0% |
| AAAACAATGGAGAGAGAAAA+AGG | - | chr7.2:1932205-1932224 | MS.gene22891:intron | 30.0% | |
| AACATGATGAAATTTGACAG+TGG | + | chr7.2:1931335-1931354 | None:intergenic | 30.0% | |
| ACTCTTCCACTAAAGTAAAA+CGG | + | chr7.2:1932115-1932134 | None:intergenic | 30.0% | |
| CAATTCAACCATAAAGTTTG+TGG | + | chr7.2:1931108-1931127 | None:intergenic | 30.0% | |
| CATGGCATGTTTAATTCATA+AGG | + | chr7.2:1931211-1931230 | None:intergenic | 30.0% | |
| GTGAGTTTCATATTCTATGA+CGG | - | chr7.2:1931721-1931740 | MS.gene22891:intron | 30.0% | |
| TCAACAAAGCTGTCATTAAA+TGG | + | chr7.2:1932154-1932173 | None:intergenic | 30.0% | |
| ! | AACCACTTTATGACAACTTT+TGG | + | chr7.2:1932277-1932296 | None:intergenic | 30.0% |
| ! | ACATTAATTGTGGAGCTTTT+CGG | + | chr7.2:1931703-1931722 | None:intergenic | 30.0% |
| ! | ATCATGATAATGTTGGATAC+AGG | - | chr7.2:1931817-1931836 | MS.gene22891:intron | 30.0% |
| ! | ATTTTCATCTACAGTTTGGT+AGG | - | chr7.2:1931255-1931274 | MS.gene22891:intron | 30.0% |
| ! | TGATGATTCATCTGATCTTT+TGG | - | chr7.2:1931938-1931957 | MS.gene22891:intron | 30.0% |
| !!! | TTTCTGGTACATTTTTTTGC+AGG | - | chr7.2:1932749-1932768 | MS.gene22891:intron | 30.0% |
| AAACAGCCAATTATACCTTG+GGG | - | chr7.2:1930963-1930982 | MS.gene22891:CDS | 35.0% | |
| AAGCAATAACATGCCAAGTT+TGG | + | chr7.2:1932497-1932516 | None:intergenic | 35.0% | |
| AAGCCTATAAACGAAAGCAA+AGG | + | chr7.2:1931653-1931672 | None:intergenic | 35.0% | |
| ACAAACAGCCAATTATACCT+TGG | - | chr7.2:1930961-1930980 | MS.gene22891:CDS | 35.0% | |
| AGCTATACATCCAAAAACCA+AGG | + | chr7.2:1931903-1931922 | None:intergenic | 35.0% | |
| CAAAATATCATTTCTCTGGG+TGG | - | chr7.2:1932299-1932318 | MS.gene22891:intron | 35.0% | |
| CAAACAGCCAATTATACCTT+GGG | - | chr7.2:1930962-1930981 | MS.gene22891:CDS | 35.0% | |
| CCAAAAGTCCACAAACTTTA+TGG | - | chr7.2:1931097-1931116 | MS.gene22891:intron | 35.0% | |
| CTCTTCCACTAAAGTAAAAC+GGG | + | chr7.2:1932114-1932133 | None:intergenic | 35.0% | |
| GACTGTTGTAAAGAAGAGAT+GGG | + | chr7.2:1932821-1932840 | None:intergenic | 35.0% | |
| GATGAAATTTGACAGTGGAA+AGG | + | chr7.2:1931330-1931349 | None:intergenic | 35.0% | |
| GTCCAAAAGTTGTCATAAAG+TGG | - | chr7.2:1932272-1932291 | MS.gene22891:CDS | 35.0% | |
| TAATGTTGGATACAGGAAGA+TGG | - | chr7.2:1931824-1931843 | MS.gene22891:intron | 35.0% | |
| TAGCCTTTGCTTTCGTTTAT+AGG | - | chr7.2:1931647-1931666 | MS.gene22891:intron | 35.0% | |
| TCAGATGAATCATCATACCA+AGG | + | chr7.2:1931933-1931952 | None:intergenic | 35.0% | |
| TCTCTGATATCCCAGTTTAT+GGG | - | chr7.2:1931979-1931998 | MS.gene22891:intron | 35.0% | |
| TGACAAGCAATAACAAACCT+TGG | + | chr7.2:1930990-1931009 | None:intergenic | 35.0% | |
| TGACTGTTGTAAAGAAGAGA+TGG | + | chr7.2:1932822-1932841 | None:intergenic | 35.0% | |
| TTCTCTGATATCCCAGTTTA+TGG | - | chr7.2:1931978-1931997 | MS.gene22891:intron | 35.0% | |
| TTGTACTTCGATGCAATTTC+AGG | - | chr7.2:1931440-1931459 | MS.gene22891:intron | 35.0% | |
| ! | AAAGTTTGTGGACTTTTGGA+GGG | + | chr7.2:1931096-1931115 | None:intergenic | 35.0% |
| ! | ACTTGGCATGTTATTGCTTT+TGG | - | chr7.2:1932498-1932517 | MS.gene22891:intron | 35.0% |
| ! | ATGTATAGCTGTTTCATCCT+TGG | - | chr7.2:1931913-1931932 | MS.gene22891:CDS | 35.0% |
| ! | ATTAGACTTTTGTACTGTGC+TGG | - | chr7.2:1932450-1932469 | MS.gene22891:intron | 35.0% |
| ! | CCATAAAGTTTGTGGACTTT+TGG | + | chr7.2:1931100-1931119 | None:intergenic | 35.0% |
| ! | TAAAGTTTGTGGACTTTTGG+AGG | + | chr7.2:1931097-1931116 | None:intergenic | 35.0% |
| !! | CTTGGCATGTTATTGCTTTT+GGG | - | chr7.2:1932499-1932518 | MS.gene22891:intron | 35.0% |
| !!! | TGACATGTTAGTTTTGTTGC+TGG | - | chr7.2:1931017-1931036 | MS.gene22891:intron | 35.0% |
| AAATGATGTGCGCATCAACT+TGG | - | chr7.2:1931480-1931499 | MS.gene22891:intron | 40.0% | |
| AACATAGTTGAGTCTGCAAG+TGG | - | chr7.2:1932789-1932808 | MS.gene22891:CDS | 40.0% | |
| AGAGAGAAAAAGGAAGAGAG+AGG | - | chr7.2:1932215-1932234 | MS.gene22891:intron | 40.0% | |
| ATTACAGAGGCCCATAAACT+GGG | + | chr7.2:1931992-1932011 | None:intergenic | 40.0% | |
| CCAAGTGGCTGATAATAGAA+TGG | + | chr7.2:1931405-1931424 | None:intergenic | 40.0% | |
| CCACTAAAGTAAAACGGGAA+AGG | + | chr7.2:1932109-1932128 | None:intergenic | 40.0% | |
| CCATTCTATTATCAGCCACT+TGG | - | chr7.2:1931402-1931421 | MS.gene22891:intron | 40.0% | |
| GCAAAGGCTAAAAACTGCAA+AGG | + | chr7.2:1931637-1931656 | None:intergenic | 40.0% | |
| ! | CCTTTCCCGTTTTACTTTAG+TGG | - | chr7.2:1932106-1932125 | MS.gene22891:CDS | 40.0% |
| !! | AAGTTTGTGGACTTTTGGAG+GGG | + | chr7.2:1931095-1931114 | None:intergenic | 40.0% |
| !! | TTTTGGAGGGGGAAATAACA+TGG | + | chr7.2:1931083-1931102 | None:intergenic | 40.0% |
| ATAACAAACCTTGGTCCCCA+AGG | + | chr7.2:1930981-1931000 | None:intergenic | 45.0% | |
| CAATTATACCTTGGGGACCA+AGG | - | chr7.2:1930970-1930989 | MS.gene22891:CDS | 45.0% | |
| CTTGGTCCCCAAGGTATAAT+TGG | + | chr7.2:1930972-1930991 | None:intergenic | 45.0% | |
| GATTACAGAGGCCCATAAAC+TGG | + | chr7.2:1931993-1932012 | None:intergenic | 45.0% | |
| TGGGATTGTAGGACCAAACT+TGG | - | chr7.2:1932481-1932500 | MS.gene22891:intron | 45.0% | |
| TTGTCTTTCTGCAGACAGCT+TGG | - | chr7.2:1931581-1931600 | MS.gene22891:intron | 45.0% | |
| ! | GGAGCTTTTCGGAGATTCAA+AGG | + | chr7.2:1931692-1931711 | None:intergenic | 45.0% |
| !! | AGTTTGTGGACTTTTGGAGG+GGG | + | chr7.2:1931094-1931113 | None:intergenic | 45.0% |
| !!! | TTGATTTTATTTAATTATTT+TGG | - | chr7.2:1931539-1931558 | MS.gene22891:intron | 5.0% |
| CAGGAAGATGGTGAAGATCC+AGG | - | chr7.2:1931836-1931855 | MS.gene22891:intron | 50.0% | |
| GATGGTGAAGATCCAGGAGA+AGG | - | chr7.2:1931842-1931861 | MS.gene22891:CDS | 50.0% | |
| GTATGGGAAAGAGACTGCTC+CGG | - | chr7.2:1931870-1931889 | MS.gene22891:CDS | 50.0% | |
| GTTTGGTCCTACAATCCCAG+TGG | + | chr7.2:1932480-1932499 | None:intergenic | 50.0% | |
| TACTGTGCTGGACTTCCACT+GGG | - | chr7.2:1932462-1932481 | MS.gene22891:intron | 50.0% | |
| TGGACTTCCACTGGGATTGT+AGG | - | chr7.2:1932470-1932489 | MS.gene22891:intron | 50.0% | |
| CAAAAACCAAGGCCAGCGAC+CGG | + | chr7.2:1931892-1931911 | None:intergenic | 55.0% | |
| CCAGGAGAAGGACTGCGTAT+GGG | - | chr7.2:1931854-1931873 | MS.gene22891:CDS | 55.0% | |
| GTACTGTGCTGGACTTCCAC+TGG | - | chr7.2:1932461-1932480 | MS.gene22891:intron | 55.0% | |
| TCCAGGAGAAGGACTGCGTA+TGG | - | chr7.2:1931853-1931872 | MS.gene22891:CDS | 55.0% | |
| TCTGCAGACAGCTTGGATCG+CGG | - | chr7.2:1931588-1931607 | MS.gene22891:intron | 55.0% | |
| AAAGAGACTGCTCCGGTCGC+TGG | - | chr7.2:1931877-1931896 | MS.gene22891:CDS | 60.0% | |
| CCCATACGCAGTCCTTCTCC+TGG | + | chr7.2:1931857-1931876 | None:intergenic | 60.0% | |
| !!! | CGGTCGCTGGCCTTGGTTTT+TGG | - | chr7.2:1931890-1931909 | MS.gene22891:CDS | 60.0% |
| ! | ACTGCTCCGGTCGCTGGCCT+TGG | - | chr7.2:1931883-1931902 | MS.gene22891:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 1930930 | 1932842 | 1930930 | ID=MS.gene22891 |
| chr7.2 | mRNA | 1930930 | 1932842 | 1930930 | ID=MS.gene22891.t1;Parent=MS.gene22891 |
| chr7.2 | exon | 1932781 | 1932842 | 1932781 | ID=MS.gene22891.t1.exon1;Parent=MS.gene22891.t1 |
| chr7.2 | CDS | 1932781 | 1932842 | 1932781 | ID=cds.MS.gene22891.t1;Parent=MS.gene22891.t1 |
| chr7.2 | exon | 1932253 | 1932310 | 1932253 | ID=MS.gene22891.t1.exon2;Parent=MS.gene22891.t1 |
| chr7.2 | CDS | 1932253 | 1932310 | 1932253 | ID=cds.MS.gene22891.t1;Parent=MS.gene22891.t1 |
| chr7.2 | exon | 1932052 | 1932177 | 1932052 | ID=MS.gene22891.t1.exon3;Parent=MS.gene22891.t1 |
| chr7.2 | CDS | 1932052 | 1932177 | 1932052 | ID=cds.MS.gene22891.t1;Parent=MS.gene22891.t1 |
| chr7.2 | exon | 1931838 | 1931933 | 1931838 | ID=MS.gene22891.t1.exon4;Parent=MS.gene22891.t1 |
| chr7.2 | CDS | 1931838 | 1931933 | 1931838 | ID=cds.MS.gene22891.t1;Parent=MS.gene22891.t1 |
| chr7.2 | exon | 1930930 | 1931001 | 1930930 | ID=MS.gene22891.t1.exon5;Parent=MS.gene22891.t1 |
| chr7.2 | CDS | 1930930 | 1931001 | 1930930 | ID=cds.MS.gene22891.t1;Parent=MS.gene22891.t1 |
| Gene Sequence |
| Protein sequence |
