Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23940.t1 | XP_003602096.2 | 89.2 | 37 | 4 | 0 | 60 | 96 | 60 | 96 | 2.10E-07 | 65.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23940.t1 | G7J5J3 | 89.2 | 37 | 4 | 0 | 60 | 96 | 60 | 96 | 1.5e-07 | 65.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049587 | MS.gene23940 | 0.81838 | 2.02E-52 | -1.69E-46 |
| MS.gene051710 | MS.gene23940 | 0.815416 | 9.35E-52 | -1.69E-46 |
| MS.gene058294 | MS.gene23940 | 0.802968 | 4.36E-49 | -1.69E-46 |
| MS.gene059276 | MS.gene23940 | 0.81407 | 1.86E-51 | -1.69E-46 |
| MS.gene059584 | MS.gene23940 | 0.814694 | 1.35E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23940.t1 | MTR_3g089100 | 93.750 | 96 | 6 | 0 | 1 | 96 | 1 | 96 | 1.78e-35 | 127 |
| MS.gene23940.t1 | MTR_3g089100 | 93.750 | 96 | 6 | 0 | 1 | 96 | 1 | 96 | 1.91e-35 | 127 |
| MS.gene23940.t1 | MTR_3g089100 | 93.750 | 96 | 6 | 0 | 1 | 96 | 1 | 96 | 2.04e-35 | 127 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCGGAGTTGTTGTTGTTGT+TGG | 0.340090 | 3.1:+88797071 | None:intergenic |
| GATGAAGCAGCACCATTATC+AGG | 0.353815 | 3.1:+88796900 | None:intergenic |
| ATTCTTTCTCAGCCTGATAA+TGG | 0.358493 | 3.1:-88796912 | MS.gene23940:intron |
| AGAAGATGAAGGTCGTTTAC+CGG | 0.409720 | 3.1:+88797053 | None:intergenic |
| CGTTTAGATGAAGGAGAAGA+AGG | 0.430320 | 3.1:+88797015 | None:intergenic |
| TCCTTCATCTAAACGCCAAA+AGG | 0.452837 | 3.1:-88797007 | MS.gene23940:intron |
| GGTTCTTGATTTGAATTCTC+CGG | 0.481752 | 3.1:+88796867 | None:intergenic |
| TATAACGAACCATCAGCAAT+CGG | 0.482608 | 3.1:+88796753 | None:intergenic |
| TCATCATCAGCAGCTGCTAC+AGG | 0.493093 | 3.1:+88796795 | None:intergenic |
| ATAACGAACCATCAGCAATC+GGG | 0.496158 | 3.1:+88796754 | None:intergenic |
| GGTTCATCGATCTGAGCATC+AGG | 0.502013 | 3.1:+88796816 | None:intergenic |
| TGATAATGGTGCTGCTTCAT+CGG | 0.517127 | 3.1:-88796898 | MS.gene23940:CDS |
| TTAGATGAAGGAGAAGAAGG+AGG | 0.556358 | 3.1:+88797018 | None:intergenic |
| TGCTTCATCGGAGAAACCAC+CGG | 0.586045 | 3.1:-88796886 | MS.gene23940:CDS |
| TAACGAACCATCAGCAATCG+GGG | 0.603967 | 3.1:+88796755 | None:intergenic |
| TTGTCATCAGAAGAAGATGA+AGG | 0.619113 | 3.1:+88797042 | None:intergenic |
| TCTTGATTTGAATTCTCCGG+TGG | 0.625267 | 3.1:+88796870 | None:intergenic |
| TCAACGAGACACAACAGTGG+TGG | 0.655270 | 3.1:-88797102 | MS.gene23940:CDS |
| AACGAACCATCAGCAATCGG+GGG | 0.689358 | 3.1:+88796756 | None:intergenic |
| GTTTCAACGAGACACAACAG+TGG | 0.761613 | 3.1:-88797105 | MS.gene23940:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAGAGAGAAAAAAGCGTAA+CGG | + | chr3.1:88796891-88796910 | None:intergenic | 30.0% |
| ATTCTTTCTCAGCCTGATAA+TGG | - | chr3.1:88796948-88796967 | MS.gene23940:intron | 35.0% | |
| TATAACGAACCATCAGCAAT+CGG | + | chr3.1:88797110-88797129 | None:intergenic | 35.0% | |
| TTGTCATCAGAAGAAGATGA+AGG | + | chr3.1:88796821-88796840 | None:intergenic | 35.0% | |
| ! | GGTTCTTGATTTGAATTCTC+CGG | + | chr3.1:88796996-88797015 | None:intergenic | 35.0% |
| !!! | ATCAGGTTGAGCACATTTTT+CGG | + | chr3.1:88797030-88797049 | None:intergenic | 35.0% |
| AGAAGATGAAGGTCGTTTAC+CGG | + | chr3.1:88796810-88796829 | None:intergenic | 40.0% | |
| ATAACGAACCATCAGCAATC+GGG | + | chr3.1:88797109-88797128 | None:intergenic | 40.0% | |
| CGTTTAGATGAAGGAGAAGA+AGG | + | chr3.1:88796848-88796867 | None:intergenic | 40.0% | |
| TCCTTCATCTAAACGCCAAA+AGG | - | chr3.1:88796853-88796872 | MS.gene23940:CDS | 40.0% | |
| TTAGATGAAGGAGAAGAAGG+AGG | + | chr3.1:88796845-88796864 | None:intergenic | 40.0% | |
| ! | ACCTTTTGGCGTTTAGATGA+AGG | + | chr3.1:88796857-88796876 | None:intergenic | 40.0% |
| ! | TCTTGATTTGAATTCTCCGG+TGG | + | chr3.1:88796993-88797012 | None:intergenic | 40.0% |
| !! | CGGATTGGAGAAAAACCTTT+TGG | + | chr3.1:88796871-88796890 | None:intergenic | 40.0% |
| !! | GAGAAAAAAGCGTAACGGAT+TGG | + | chr3.1:88796886-88796905 | None:intergenic | 40.0% |
| !! | TGATAATGGTGCTGCTTCAT+CGG | - | chr3.1:88796962-88796981 | MS.gene23940:intron | 40.0% |
| !!! | TCAGGTTGAGCACATTTTTC+GGG | + | chr3.1:88797029-88797048 | None:intergenic | 40.0% |
| !!! | TTGAGCACATTTTTCGGGAT+CGG | + | chr3.1:88797024-88797043 | None:intergenic | 40.0% |
| CCCAACAACAACAACAACTC+CGG | - | chr3.1:88796788-88796807 | MS.gene23940:CDS | 45.0% | |
| GATGAAGCAGCACCATTATC+AGG | + | chr3.1:88796963-88796982 | None:intergenic | 45.0% | |
| GTTTCAACGAGACACAACAG+TGG | - | chr3.1:88796755-88796774 | MS.gene23940:CDS | 45.0% | |
| TAACGAACCATCAGCAATCG+GGG | + | chr3.1:88797108-88797127 | None:intergenic | 45.0% | |
| ! | ACCGGAGTTGTTGTTGTTGT+TGG | + | chr3.1:88796792-88796811 | None:intergenic | 45.0% |
| ! | CCGGAGTTGTTGTTGTTGTT+GGG | + | chr3.1:88796791-88796810 | None:intergenic | 45.0% |
| ! | CGGAGTTGTTGTTGTTGTTG+GGG | + | chr3.1:88796790-88796809 | None:intergenic | 45.0% |
| AACGAACCATCAGCAATCGG+GGG | + | chr3.1:88797107-88797126 | None:intergenic | 50.0% | |
| GGTTCATCGATCTGAGCATC+AGG | + | chr3.1:88797047-88797066 | None:intergenic | 50.0% | |
| TCAACGAGACACAACAGTGG+TGG | - | chr3.1:88796758-88796777 | MS.gene23940:CDS | 50.0% | |
| TCATCATCAGCAGCTGCTAC+AGG | + | chr3.1:88797068-88797087 | None:intergenic | 50.0% | |
| TGCTTCATCGGAGAAACCAC+CGG | - | chr3.1:88796974-88796993 | MS.gene23940:intron | 50.0% | |
| !!! | CATTTTTCGGGATCGGCAGA+CGG | + | chr3.1:88797017-88797036 | None:intergenic | 50.0% |
| AGCAATCGGGGGAGTTGCAT+CGG | + | chr3.1:88797096-88797115 | None:intergenic | 55.0% | |
| GCAACTCCCCCGATTGCTGA+TGG | - | chr3.1:88797098-88797117 | MS.gene23940:CDS | 60.0% | |
| GCAATCGGGGGAGTTGCATC+GGG | + | chr3.1:88797095-88797114 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 88796752 | 88797130 | 88796752 | ID=MS.gene23940 |
| chr3.1 | mRNA | 88796752 | 88797130 | 88796752 | ID=MS.gene23940.t1;Parent=MS.gene23940 |
| chr3.1 | exon | 88797008 | 88797130 | 88797008 | ID=MS.gene23940.t1.exon1;Parent=MS.gene23940.t1 |
| chr3.1 | CDS | 88797008 | 88797130 | 88797008 | ID=cds.MS.gene23940.t1;Parent=MS.gene23940.t1 |
| chr3.1 | exon | 88796752 | 88796922 | 88796752 | ID=MS.gene23940.t1.exon2;Parent=MS.gene23940.t1 |
| chr3.1 | CDS | 88796752 | 88796922 | 88796752 | ID=cds.MS.gene23940.t1;Parent=MS.gene23940.t1 |
| Gene Sequence |
| Protein sequence |
