Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24235.t1 | XP_003589327.1 | 99 | 405 | 4 | 0 | 125 | 529 | 110 | 514 | 5.30E-220 | 773.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24235.t1 | D9R4W7 | 44.1 | 392 | 210 | 5 | 139 | 529 | 11 | 394 | 4.2e-80 | 300.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24235.t1 | G7I5C6 | 99.0 | 405 | 4 | 0 | 125 | 529 | 110 | 514 | 3.8e-220 | 773.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050670 | MS.gene24235 | 0.826272 | 2.97E-54 | -1.69E-46 |
| MS.gene051525 | MS.gene24235 | 0.80027 | 1.56E-48 | -1.69E-46 |
| MS.gene052188 | MS.gene24235 | 0.809799 | 1.58E-50 | -1.69E-46 |
| MS.gene052414 | MS.gene24235 | 0.813018 | 3.16E-51 | -1.69E-46 |
| MS.gene052982 | MS.gene24235 | 0.803362 | 3.61E-49 | -1.69E-46 |
| MS.gene053928 | MS.gene24235 | 0.809543 | 1.80E-50 | -1.69E-46 |
| MS.gene054183 | MS.gene24235 | 0.8109 | 9.16E-51 | -1.69E-46 |
| MS.gene054719 | MS.gene24235 | 0.811345 | 7.34E-51 | -1.69E-46 |
| MS.gene054920 | MS.gene24235 | 0.811252 | 7.68E-51 | -1.69E-46 |
| MS.gene055369 | MS.gene24235 | 0.831695 | 1.44E-55 | -1.69E-46 |
| MS.gene055539 | MS.gene24235 | 0.806512 | 7.93E-50 | -1.69E-46 |
| MS.gene05553 | MS.gene24235 | 0.843995 | 9.94E-59 | -1.69E-46 |
| MS.gene056407 | MS.gene24235 | 0.814448 | 1.53E-51 | -1.69E-46 |
| MS.gene056497 | MS.gene24235 | 0.813242 | 2.83E-51 | -1.69E-46 |
| MS.gene056514 | MS.gene24235 | 0.814025 | 1.90E-51 | -1.69E-46 |
| MS.gene056723 | MS.gene24235 | 0.845019 | 5.27E-59 | -1.69E-46 |
| MS.gene057124 | MS.gene24235 | 0.816268 | 6.04E-52 | -1.69E-46 |
| MS.gene057749 | MS.gene24235 | 0.81296 | 3.26E-51 | -1.69E-46 |
| MS.gene057823 | MS.gene24235 | 0.826553 | 2.55E-54 | -1.69E-46 |
| MS.gene058700 | MS.gene24235 | 0.821355 | 4.22E-53 | -1.69E-46 |
| MS.gene058949 | MS.gene24235 | 0.802193 | 6.30E-49 | -1.69E-46 |
| MS.gene059420 | MS.gene24235 | 0.833236 | 5.99E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24235.t1 | MTR_1g022980 | 92.467 | 531 | 21 | 4 | 1 | 529 | 1 | 514 | 0.0 | 881 |
| MS.gene24235.t1 | MTR_5g089970 | 38.462 | 299 | 148 | 7 | 192 | 457 | 107 | 402 | 4.37e-45 | 167 |
| MS.gene24235.t1 | MTR_5g089970 | 38.014 | 292 | 145 | 7 | 199 | 457 | 106 | 394 | 1.10e-41 | 158 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24235.t1 | AT5G57960 | 83.610 | 421 | 63 | 1 | 109 | 529 | 90 | 504 | 0.0 | 715 |
| MS.gene24235.t1 | AT5G57960 | 86.782 | 348 | 46 | 0 | 182 | 529 | 3 | 350 | 0.0 | 642 |
| MS.gene24235.t1 | AT3G49725 | 35.535 | 318 | 166 | 8 | 176 | 457 | 157 | 471 | 1.90e-41 | 158 |
Find 108 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTTTGTTACCATTTGAAAA+TGG | 0.177139 | 1.2:+1876751 | MS.gene24235:CDS |
| AGCTGATACTGCTGGATTAT+TGG | 0.256175 | 1.2:+1871131 | MS.gene24235:CDS |
| AGAAGATTCTTTGAGTGAAT+TGG | 0.301356 | 1.2:+1871101 | MS.gene24235:CDS |
| GATATCAGAGTCATCACTTT+TGG | 0.326042 | 1.2:+1874907 | MS.gene24235:CDS |
| ACCGAAAGCAGTACCGTAAC+AGG | 0.336454 | 1.2:+1874064 | MS.gene24235:CDS |
| CTTTGTTACCATTTGAAAAT+GGG | 0.348300 | 1.2:+1876752 | MS.gene24235:CDS |
| CCGATATACGTCCTAGGGTT+TGG | 0.363874 | 1.2:-1872434 | None:intergenic |
| GCCTGTTACGGTACTGCTTT+CGG | 0.368909 | 1.2:-1874065 | None:intergenic |
| AACCATTCCAACTTGGTGTA+TGG | 0.370214 | 1.2:-1876788 | None:intergenic |
| TAAAACCAACAGTGTCTGTT+AGG | 0.378664 | 1.2:-1874706 | None:intergenic |
| CATTTATCTGTTGCTCAGCC+AGG | 0.379878 | 1.2:-1875076 | None:intergenic |
| AACACATGAAGCATCTTTAC+AGG | 0.379979 | 1.2:+1873216 | MS.gene24235:CDS |
| TGGTGCATGTGATTGATATC+AGG | 0.385889 | 1.2:+1874927 | MS.gene24235:CDS |
| CTAGGTTGCATTAGCACAAA+TGG | 0.387279 | 1.2:+1873668 | MS.gene24235:intron |
| TTGTATGCCATTGCCACTTA+AGG | 0.397151 | 1.2:-1876573 | None:intergenic |
| CCAAACCCTAGGACGTATAT+CGG | 0.399038 | 1.2:+1872434 | MS.gene24235:CDS |
| TTACATGTTTAAGAAAGTTA+AGG | 0.413739 | 1.2:-1877033 | None:intergenic |
| GATTGTTCTTGTTCTTGAAT+TGG | 0.418768 | 1.2:-1870245 | None:intergenic |
| GGTGAAGTTAGTGATTCATT+TGG | 0.426485 | 1.2:+1871075 | MS.gene24235:CDS |
| CAATTCCAAAGTTGATGGTC+TGG | 0.428100 | 1.2:+1875135 | MS.gene24235:CDS |
| GGTAGTGGCTTTATCTTCAT+CGG | 0.428638 | 1.2:-1870282 | None:intergenic |
| ATGAAATGCGCATGCGTTAA+CGG | 0.432671 | 1.2:+1869987 | MS.gene24235:CDS |
| GTTAAGAGAGAAAGAGGATA+AGG | 0.435361 | 1.2:-1870135 | None:intergenic |
| ATCTTTCTTACTAGTGTAGT+AGG | 0.443695 | 1.2:-1874740 | None:intergenic |
| GGAGGACAGGTGAAGGGAAT+GGG | 0.443918 | 1.2:+1873744 | MS.gene24235:CDS |
| TAAGAGTGCAATTAATGCAT+TGG | 0.446596 | 1.2:+1872478 | MS.gene24235:CDS |
| ATTTATCTGTTGCTCAGCCA+GGG | 0.448336 | 1.2:-1875075 | None:intergenic |
| TTCTTGAATTGGAAGTAAAA+CGG | 0.448597 | 1.2:-1870234 | None:intergenic |
| GGTACACACAGTTGTCTCAT+AGG | 0.449520 | 1.2:-1877006 | None:intergenic |
| GCTTTATCTTCATCGGGAGT+TGG | 0.450309 | 1.2:-1870275 | None:intergenic |
| GTAGTGGCTTTATCTTCATC+GGG | 0.451549 | 1.2:-1870281 | None:intergenic |
| ACCTGTGCCAGTGGTATCCT+TGG | 0.453180 | 1.2:+1874098 | MS.gene24235:CDS |
| TGGAAGTAAAACGGCGTCGT+TGG | 0.454503 | 1.2:-1870225 | None:intergenic |
| TAGGTTGGTTATACGAATGC+TGG | 0.454748 | 1.2:+1874220 | MS.gene24235:intron |
| ATCATCAATTCCAAAGTTGA+TGG | 0.461956 | 1.2:+1875130 | MS.gene24235:CDS |
| ACTCATCTTGAGCGTCAATC+AGG | 0.469943 | 1.2:+1873723 | MS.gene24235:CDS |
| TTTGATGATGAGCTATCAGC+TGG | 0.475329 | 1.2:+1872518 | MS.gene24235:CDS |
| TTTAACAGGACTCCATGGTA+TGG | 0.475571 | 1.2:+1876722 | MS.gene24235:intron |
| CCAGAGCCGATATACGTCCT+AGG | 0.479202 | 1.2:-1872440 | None:intergenic |
| CTCCTCCAGTGTGGCTCTGA+AGG | 0.482448 | 1.2:-1874885 | None:intergenic |
| AGGAGGACAGGTGAAGGGAA+TGG | 0.488898 | 1.2:+1873743 | MS.gene24235:CDS |
| TTATCTTCATCGGGAGTTGG+AGG | 0.492319 | 1.2:-1870272 | None:intergenic |
| TTCTAGGCAACTGCGCAACC+TGG | 0.497117 | 1.2:+1873117 | MS.gene24235:intron |
| TTGTTCCAGACCATCAACTT+TGG | 0.497637 | 1.2:-1875140 | None:intergenic |
| GCAACTGCGCAACCTGGAAA+AGG | 0.498140 | 1.2:+1873123 | MS.gene24235:CDS |
| TTTCCTATGGCAGATTGATA+GGG | 0.500022 | 1.2:+1876487 | MS.gene24235:intron |
| ACCTGTACTCTCCTTGTAGT+TGG | 0.500127 | 1.2:-1874316 | None:intergenic |
| CTCGCTGATTAAATATATCA+AGG | 0.502133 | 1.2:-1873189 | None:intergenic |
| AACAAACCTCTCTTCCGTTA+CGG | 0.507246 | 1.2:-1870376 | None:intergenic |
| TGCGCATGCGTTAACGGTGG+TGG | 0.508686 | 1.2:+1869993 | MS.gene24235:CDS |
| TTCCGTTACGGAGCTTGAAG+CGG | 0.515448 | 1.2:-1870364 | None:intergenic |
| GAGCAGTTAGCTGATACTGC+TGG | 0.517824 | 1.2:+1871123 | MS.gene24235:CDS |
| CAGAAAATCAGACTAGAAGC+TGG | 0.519095 | 1.2:+1876521 | MS.gene24235:CDS |
| CCTAGGACGTATATCGGCTC+TGG | 0.521174 | 1.2:+1872440 | MS.gene24235:CDS |
| TGCTGCCTTCAGAGCCACAC+TGG | 0.528376 | 1.2:+1874880 | MS.gene24235:CDS |
| TCAATCAGGAGGACAGGTGA+AGG | 0.530450 | 1.2:+1873737 | MS.gene24235:CDS |
| CAACTTTAGATCCAACTACA+AGG | 0.530983 | 1.2:+1874305 | MS.gene24235:CDS |
| GTACTGAGAGACGCCTGTTA+CGG | 0.534214 | 1.2:-1874077 | None:intergenic |
| TTAACAGGACTCCATGGTAT+GGG | 0.535370 | 1.2:+1876723 | MS.gene24235:intron |
| TGTAACGTTAAGAGAGAAAG+AGG | 0.539755 | 1.2:-1870141 | None:intergenic |
| AGTTGCAAATAGTTTATCCT+CGG | 0.544687 | 1.2:-1874287 | None:intergenic |
| ACCAAGGATACCACTGGCAC+AGG | 0.550754 | 1.2:-1874099 | None:intergenic |
| TGAGCGTCAATCAGGAGGAC+AGG | 0.551278 | 1.2:+1873731 | MS.gene24235:CDS |
| CGAGCAAGGGACATATATCA+AGG | 0.553171 | 1.2:+1876948 | MS.gene24235:CDS |
| AACCGCTTCAAGCTCCGTAA+CGG | 0.554129 | 1.2:+1870362 | MS.gene24235:CDS |
| TATTCTTTAACAGGACTCCA+TGG | 0.559078 | 1.2:+1876717 | MS.gene24235:intron |
| TTTGTTACCATTTGAAAATG+GGG | 0.559718 | 1.2:+1876753 | MS.gene24235:CDS |
| CACCATACACCAAGTTGGAA+TGG | 0.561527 | 1.2:+1876786 | MS.gene24235:CDS |
| CAGAGCCGATATACGTCCTA+GGG | 0.562024 | 1.2:-1872439 | None:intergenic |
| CTGGTTGGTGTTGAGAGAAA+AGG | 0.565144 | 1.2:+1871054 | MS.gene24235:CDS |
| TTTATCTGTTGCTCAGCCAG+GGG | 0.567413 | 1.2:-1875074 | None:intergenic |
| ATATAGGAATATACCGAGCA+AGG | 0.568191 | 1.2:+1876934 | MS.gene24235:CDS |
| CTTGGTGTATGGTGCTGAGA+AGG | 0.572248 | 1.2:-1876777 | None:intergenic |
| TGCAGTTCAAGACAAACTAA+AGG | 0.573803 | 1.2:+1876607 | MS.gene24235:CDS |
| CTCAGCACCATACACCAAGT+TGG | 0.574248 | 1.2:+1876781 | MS.gene24235:CDS |
| TCCAACTACAAGGAGAGTAC+AGG | 0.574258 | 1.2:+1874315 | MS.gene24235:CDS |
| TAAGCAACCTCGCATAACGC+AGG | 0.575527 | 1.2:-1876981 | None:intergenic |
| TGTCCAGATGAAGAATGGAA+AGG | 0.575689 | 1.2:+1874676 | MS.gene24235:intron |
| GTATGCATATCTGCCTTAAG+TGG | 0.578178 | 1.2:+1876560 | MS.gene24235:CDS |
| GAAGAGAAAGCGTATCTGGT+TGG | 0.578497 | 1.2:+1871039 | MS.gene24235:CDS |
| GTTTAAGAAAGTTAAGGTCG+TGG | 0.579779 | 1.2:-1877027 | None:intergenic |
| ATATCTGCCTTAAGTGGCAA+TGG | 0.584431 | 1.2:+1876566 | MS.gene24235:CDS |
| AAAGTTGATGGTCTGGAACA+AGG | 0.588369 | 1.2:+1875142 | MS.gene24235:CDS |
| CTTGATATATGTCCCTTGCT+CGG | 0.592539 | 1.2:-1876947 | None:intergenic |
| CAAGCTCCGTAACGGAAGAG+AGG | 0.609194 | 1.2:+1870370 | MS.gene24235:CDS |
| AAGCAACCTCGCATAACGCA+GGG | 0.609480 | 1.2:-1876980 | None:intergenic |
| TATAGGAATATACCGAGCAA+GGG | 0.610221 | 1.2:+1876935 | MS.gene24235:CDS |
| AAACTAACCAAGGATACCAC+TGG | 0.610650 | 1.2:-1874105 | None:intergenic |
| TTTCTCCTAACAGACACTGT+TGG | 0.614231 | 1.2:+1874701 | MS.gene24235:CDS |
| GGTATACTTCTGGTAAGTAG+AGG | 0.616128 | 1.2:-1871160 | None:intergenic |
| TGTTGTTACTGTTATCGTCG+TGG | 0.617453 | 1.2:-1870334 | None:intergenic |
| ACAGGACTCCATGGTATGGG+TGG | 0.618988 | 1.2:+1876726 | MS.gene24235:intron |
| CAATCAGGAGGACAGGTGAA+GGG | 0.620912 | 1.2:+1873738 | MS.gene24235:CDS |
| CAAAGCTTCCACCCATACCA+TGG | 0.623436 | 1.2:-1876734 | None:intergenic |
| TTATCTGTTGCTCAGCCAGG+GGG | 0.626944 | 1.2:-1875073 | None:intergenic |
| GGCTTGCTTCTCCAAACCCT+AGG | 0.630711 | 1.2:+1872423 | MS.gene24235:intron |
| CTCTGATATCTCCTCCAGTG+TGG | 0.631450 | 1.2:-1874894 | None:intergenic |
| TGGAGCAGATGTTCTTGCCG+AGG | 0.641917 | 1.2:+1874270 | MS.gene24235:CDS |
| TGAAGTTCTTCAGTGTTGTG+CGG | 0.644930 | 1.2:-1870164 | None:intergenic |
| TGCCTTCAGAGCCACACTGG+AGG | 0.649520 | 1.2:+1874883 | MS.gene24235:CDS |
| TGATATATTTAATCAGCGAG+CGG | 0.651334 | 1.2:+1873192 | MS.gene24235:CDS |
| CATCTTGAGCGTCAATCAGG+AGG | 0.655959 | 1.2:+1873726 | MS.gene24235:CDS |
| TCTCTCAGTACCTGTGCCAG+TGG | 0.658454 | 1.2:+1874089 | MS.gene24235:CDS |
| ATGTGCCCCTGCGTTATGCG+AGG | 0.667647 | 1.2:+1876974 | MS.gene24235:CDS |
| TTGATGATGAGCTATCAGCT+GGG | 0.694886 | 1.2:+1872519 | MS.gene24235:CDS |
| AGCAACCTCGCATAACGCAG+GGG | 0.713675 | 1.2:-1876979 | None:intergenic |
| TGAGCAACAGATAAATGCTG+TGG | 0.729821 | 1.2:+1875082 | MS.gene24235:CDS |
| AAATGCGCATGCGTTAACGG+TGG | 0.787822 | 1.2:+1869990 | MS.gene24235:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 1869984 | 1877055 | 1869984 | ID=MS.gene24235 |
| chr1.2 | mRNA | 1869984 | 1877055 | 1869984 | ID=MS.gene24235.t1;Parent=MS.gene24235 |
| chr1.2 | exon | 1869984 | 1870391 | 1869984 | ID=MS.gene24235.t1.exon1;Parent=MS.gene24235.t1 |
| chr1.2 | CDS | 1869984 | 1870391 | 1869984 | ID=cds.MS.gene24235.t1;Parent=MS.gene24235.t1 |
| chr1.2 | exon | 1871033 | 1871175 | 1871033 | ID=MS.gene24235.t1.exon2;Parent=MS.gene24235.t1 |
| chr1.2 | CDS | 1871033 | 1871175 | 1871033 | ID=cds.MS.gene24235.t1;Parent=MS.gene24235.t1 |
| chr1.2 | exon | 1872424 | 1872540 | 1872424 | ID=MS.gene24235.t1.exon3;Parent=MS.gene24235.t1 |
| chr1.2 | CDS | 1872424 | 1872540 | 1872424 | ID=cds.MS.gene24235.t1;Parent=MS.gene24235.t1 |
| chr1.2 | exon | 1873123 | 1873237 | 1873123 | ID=MS.gene24235.t1.exon4;Parent=MS.gene24235.t1 |
| chr1.2 | CDS | 1873123 | 1873237 | 1873123 | ID=cds.MS.gene24235.t1;Parent=MS.gene24235.t1 |
| chr1.2 | exon | 1873672 | 1873809 | 1873672 | ID=MS.gene24235.t1.exon5;Parent=MS.gene24235.t1 |
| chr1.2 | CDS | 1873672 | 1873809 | 1873672 | ID=cds.MS.gene24235.t1;Parent=MS.gene24235.t1 |
| chr1.2 | exon | 1874024 | 1874119 | 1874024 | ID=MS.gene24235.t1.exon6;Parent=MS.gene24235.t1 |
| chr1.2 | CDS | 1874024 | 1874119 | 1874024 | ID=cds.MS.gene24235.t1;Parent=MS.gene24235.t1 |
| chr1.2 | exon | 1874223 | 1874336 | 1874223 | ID=MS.gene24235.t1.exon7;Parent=MS.gene24235.t1 |
| chr1.2 | CDS | 1874223 | 1874336 | 1874223 | ID=cds.MS.gene24235.t1;Parent=MS.gene24235.t1 |
| chr1.2 | exon | 1874683 | 1874751 | 1874683 | ID=MS.gene24235.t1.exon8;Parent=MS.gene24235.t1 |
| chr1.2 | CDS | 1874683 | 1874751 | 1874683 | ID=cds.MS.gene24235.t1;Parent=MS.gene24235.t1 |
| chr1.2 | exon | 1874878 | 1874948 | 1874878 | ID=MS.gene24235.t1.exon9;Parent=MS.gene24235.t1 |
| chr1.2 | CDS | 1874878 | 1874948 | 1874878 | ID=cds.MS.gene24235.t1;Parent=MS.gene24235.t1 |
| chr1.2 | exon | 1875070 | 1875163 | 1875070 | ID=MS.gene24235.t1.exon10;Parent=MS.gene24235.t1 |
| chr1.2 | CDS | 1875070 | 1875163 | 1875070 | ID=cds.MS.gene24235.t1;Parent=MS.gene24235.t1 |
| chr1.2 | exon | 1876500 | 1876628 | 1876500 | ID=MS.gene24235.t1.exon11;Parent=MS.gene24235.t1 |
| chr1.2 | CDS | 1876500 | 1876628 | 1876500 | ID=cds.MS.gene24235.t1;Parent=MS.gene24235.t1 |
| chr1.2 | exon | 1876730 | 1876819 | 1876730 | ID=MS.gene24235.t1.exon12;Parent=MS.gene24235.t1 |
| chr1.2 | CDS | 1876730 | 1876819 | 1876730 | ID=cds.MS.gene24235.t1;Parent=MS.gene24235.t1 |
| chr1.2 | exon | 1876927 | 1877055 | 1876927 | ID=MS.gene24235.t1.exon13;Parent=MS.gene24235.t1 |
| chr1.2 | CDS | 1876927 | 1877055 | 1876927 | ID=cds.MS.gene24235.t1;Parent=MS.gene24235.t1 |
| Gene Sequence |
| Protein sequence |
