AlfalfaGEDB | MS.gene24281

M. sativa cultivar XinJiangDaYe / MS.gene24281

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008168 GO:0016740 GO:0016741
PFAM: PF03141.19

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24281.t1	XP_013466100.1	90.6	705	62	2	1	705	1	701	0.00E+00	1214.9

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24281.t1	Q9LN50	66.4	715	230	2	1	705	10	724	9.2e-255	880.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24281.t1	A0A072VPS8	90.6	705	62	2	1	705	1	701	0.0e+00	1214.9

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049825	MS.gene24281	0.80153	8.62E-49	-1.69E-46
MS.gene052302	MS.gene24281	0.812764	3.60E-51	-1.69E-46
MS.gene052376	MS.gene24281	0.810891	9.20E-51	-1.69E-46
MS.gene053294	MS.gene24281	0.903502	3.25E-79	-1.69E-46
MS.gene053401	MS.gene24281	0.822417	2.40E-53	-1.69E-46
MS.gene055555	MS.gene24281	0.806636	7.47E-50	-1.69E-46
MS.gene055808	MS.gene24281	0.826317	2.90E-54	-1.69E-46
MS.gene056091	MS.gene24281	0.835162	1.97E-56	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24281.t1	MTR_1g022255	97.021	705	17	3	1	705	1	701	0.0	1412
MS.gene24281.t1	MTR_1g022255	95.222	586	22	4	1	584	1	582	0.0	1033
MS.gene24281.t1	MTR_8g093880	43.639	676	339	10	58	705	147	808	0.0	599
MS.gene24281.t1	MTR_3g096480	50.473	529	240	5	197	705	252	778	0.0	592
MS.gene24281.t1	MTR_3g088790	40.878	729	377	13	13	705	34	744	0.0	563
MS.gene24281.t1	MTR_5g021100	40.313	702	372	14	38	705	147	835	0.0	554
MS.gene24281.t1	MTR_8g076650	45.785	522	264	7	200	705	374	892	6.15e-175	523
MS.gene24281.t1	MTR_5g083150	43.248	548	290	8	172	705	60	600	5.68e-162	480
MS.gene24281.t1	MTR_3g071530	43.585	530	269	11	195	705	130	648	2.74e-149	449
MS.gene24281.t1	MTR_5g080720	42.240	509	257	14	202	686	124	619	4.83e-126	389

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
MS.gene24281.t1	AT1G19430	62.117	718	256	4	1	705	10	724	930
MS.gene24281.t1	AT1G19430	62.117	718	256	4	1	705	10	724	930
MS.gene24281.t1	AT1G19430	58.844	588	226	4	1	575	10	594	706
MS.gene24281.t1	AT5G64030	42.183	678	356	8	50	705	155	818	585
MS.gene24281.t1	AT1G29470	40.999	761	374	17	8	705	11	759	571
MS.gene24281.t1	AT1G29470	40.999	761	374	17	8	705	11	759	571
MS.gene24281.t1	AT2G34300	39.414	751	400	8	8	705	11	759	570
MS.gene24281.t1	AT2G34300	39.414	751	400	8	8	705	11	759	570
MS.gene24281.t1	AT2G34300	39.414	751	400	8	8	705	11	759	570
MS.gene24281.t1	AT3G51070	47.228	523	254	6	203	705	371	891	560

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Find 148 sgRNAs with CRISPR-Local

Find 334 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTATGGGAAGCGTATC+CGG	0.395966	1.1:-2065341	None:intergenic
AAAGGTGCAGTACCGGCACA+CGG	0.659564	1.1:+2061753	MS.gene24281:CDS
AAATATTGAGAAGTATGCAA+TGG	0.561804	1.1:+2065931	MS.gene24281:CDS
AACTATGGCGTTCCAATGGT+TGG	0.540107	1.1:-2064913	None:intergenic
AACTTCTACTGGAGATGAAT+CGG	0.456963	1.1:+2063433	MS.gene24281:CDS
AAGAGGAACCATACACATGA+AGG	0.545446	1.1:-2061797	None:intergenic
AAGGATGCCCTTCATGTGTA+TGG	0.465411	1.1:+2061789	MS.gene24281:CDS
AAGTCATATCTCAATGGAAT+GGG	0.482780	1.1:+2064939	MS.gene24281:CDS
AATAAAGAGAATGTGAATCA+AGG	0.469706	1.1:+2061448	MS.gene24281:CDS
AATATTGAGAAGTATGCAAT+GGG	0.493277	1.1:+2065932	MS.gene24281:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACATGTAGATATTGCAAG+AGG	+	chr1.1:2062410-2062429	MS.gene24281:intron	30.0%
AAACGCTAGTATAATGGTTA+TGG	-	chr1.1:2065532-2065551	None:intergenic	30.0%
AAATCCCCGTTCAAAAATTA+TGG	-	chr1.1:2065357-2065376	None:intergenic	30.0%
AAATCCTAGTAGAAGCTTAT+AGG	-	chr1.1:2062320-2062339	None:intergenic	30.0%
AAGTCATATCTCAATGGAAT+GGG	+	chr1.1:2064939-2064958	MS.gene24281:CDS	30.0%
AATCCCCGTTCAAAAATTAT+GGG	-	chr1.1:2065356-2065375	None:intergenic	30.0%
AATCCTAGTAGAAGCTTATA+GGG	-	chr1.1:2062319-2062338	None:intergenic	30.0%
AGAATATATCAAAAGCCTGA+AGG	+	chr1.1:2064584-2064603	MS.gene24281:CDS	30.0%
AGATGTTGGAAATCATTCAA+TGG	-	chr1.1:2065212-2065231	None:intergenic	30.0%
AGGTTCATTTGTTTGATTCT+AGG	-	chr1.1:2064051-2064070	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	2061079	2066025	2061079	ID=MS.gene24281
chr1.1	mRNA	2061079	2066025	2061079	ID=MS.gene24281.t1;Parent=MS.gene24281
chr1.1	exon	2061079	2061870	2061079	ID=MS.gene24281.t1.exon1;Parent=MS.gene24281.t1
chr1.1	CDS	2061079	2061870	2061079	ID=cds.MS.gene24281.t1;Parent=MS.gene24281.t1
chr1.1	exon	2062590	2062739	2062590	ID=MS.gene24281.t1.exon2;Parent=MS.gene24281.t1
chr1.1	CDS	2062590	2062739	2062590	ID=cds.MS.gene24281.t1;Parent=MS.gene24281.t1
chr1.1	exon	2063042	2063312	2063042	ID=MS.gene24281.t1.exon3;Parent=MS.gene24281.t1
chr1.1	CDS	2063042	2063312	2063042	ID=cds.MS.gene24281.t1;Parent=MS.gene24281.t1
chr1.1	exon	2063427	2063519	2063427	ID=MS.gene24281.t1.exon4;Parent=MS.gene24281.t1
chr1.1	CDS	2063427	2063519	2063427	ID=cds.MS.gene24281.t1;Parent=MS.gene24281.t1
chr1.1	exon	2064507	2064681	2064507	ID=MS.gene24281.t1.exon5;Parent=MS.gene24281.t1
chr1.1	CDS	2064507	2064681	2064507	ID=cds.MS.gene24281.t1;Parent=MS.gene24281.t1
chr1.1	exon	2064773	2065015	2064773	ID=MS.gene24281.t1.exon6;Parent=MS.gene24281.t1
chr1.1	CDS	2064773	2065015	2064773	ID=cds.MS.gene24281.t1;Parent=MS.gene24281.t1
chr1.1	exon	2065280	2065474	2065280	ID=MS.gene24281.t1.exon7;Parent=MS.gene24281.t1
chr1.1	CDS	2065280	2065474	2065280	ID=cds.MS.gene24281.t1;Parent=MS.gene24281.t1
chr1.1	exon	2065827	2066025	2065827	ID=MS.gene24281.t1.exon8;Parent=MS.gene24281.t1
chr1.1	CDS	2065827	2066025	2065827	ID=cds.MS.gene24281.t1;Parent=MS.gene24281.t1

Gene Sequence

>MS.gene24281

ATGGCTATTATACCTCGTCAAGCTAAAAGACCAACTGGGTTATGGGTGAAGATGACAGCTGTAACAATTTTGGGTCTCTGTTTTATTTTTGTTTGGACTGTGTTTTCTTCTTCATCTTCTTCTGTTAGTTTTCAAAGGGAAAGTTTTGAAGATATTTCAGAACCTGTTTCTTCTTCTTCTTCTTCTTCCAATGTTAAAAAACCAACACCAAAATCAGAAAAACGCGTGGAAGTGGAGAAAGATCATAAATTTCATAAGAGTGATGAGAAAAAGATTGATGATGGGTCATCTTCAAATTCTTCTTCTACGCGCCCTCATAAGAAAGAAGTGGCACGTGAGAAGAAACGTGTGCATAAGAAGGAGGATAATAATAAAGAGAATGTGAATCAAGGGTCATTGGAGAAAGAGGAAGAAGGTGAAAAAGAGTTGGAAGATAAAGAAGAAGGATTGGATCATGAGAGTGAAGTTGATTTGGATTCTGATGTTGTTGGTGGTGATGATTCAGCTGAATCCGTCGATGAAGATTCTGAGATATTGGAAGAGTTAAAGAAAACAAAGAAGGGAAAAGTGAAAGGACCTTTGTTTGAATCAAATGTTAACTATAGATGGAAAATGTGCAATACTAGAAGCAAGCATAACTATATTCCTTGCATTGATATCGAAGTAGGTGGTGGAAAGGTGCAGTACCGGCACACGGAGAGGAGTTGTCCAAGGATGCCCTTCATGTGTATGGTTCCTCTTCCTCTTGATGGTTATGAGTCTCCGGTGCCGTGGCCTGAGAGCAAATTGAAGGTATATGAAAAGTATGTTATATTTAAGGTCTAGTTTGGATTAGCTTTTAGATTGTTGGAGGTTATGTGTTAATGATTTTTCACTTGTGAAATAATCCATATAAGCTTATAGTATGGAGAATTTAGGGCACATTTGAATTAGATTATTTGAGCTTATTTATTAGCATGAGAACTTGTGAGACTGTTTGCGAGAGCTAATGGAAACAACTTATGACTTGTTCATAAGCTGTTTTAACTTATTCCCATAAACTCTCAAATAAGCTTATGAAAATAGTTTAACTTTATCTTATCTTTTGTTATAGAAATATCTTATACATAAGCCATTATCATATAATTGCTTACGTTATAAGCACTTAATTAAGTTGTTTAGCCTACTAGGGTCTGGTTTGGATTAGCTTCTAGATTATGATACTTATGAGTTTATGATTCTTCATTTTTGATATAATCCCTATAAGCTTCTACTAGGATTTATTGAGAAGTTAGAGATAGCATATGCATTGATATAGAGAAGCTATTTTCTTTTTGAAACTATTGTTTAAAAAACATGTAGATATTGCAAGAGGCTTTTAATATTAATTTTTCAGTAAATTTTCATTTATTAGTCGGATAAGGCTCGGCTGTTGTTGATGTTCATTTAGAAGTTTATCTAGTCTAGCCTTAAGTGTTGGATTGGTTTGGTGCTTCTTTGTTATCTCACAGTAACATTTGGCGTCAGTGCAGATATTTTATAAGAATGTTGCACACCCGAAACTAGCTGCATATATTAAAAAACATCGTTGGTTGGTGGATTCTGGAGAATATCTTACTTTCCCTCAAAACCAGTCAGAATTTTTGGGAGGGATTCAGCACTATCTTGAGTCGATTGAAGAGGTGAGATTTCTATTAGAATGATGCTTGTTTGTTGGTTCCTCTTAAACATAATCATTGTATTGCAAATGATAGAACATTAGTTAATAATGGATAATTTCAGTTTCCAAAACATAGTCTCATTTCTTTGGATATGACGAGATGAAAATGTATCTCTGTGGCACTGTGATTTCTAGGTTTGACTTGTTTATGATTTGCTTCGTAACAACTGTTTATGATTTTCAACTCAACAGATTTTTTTTTTTTGTTAATATTCACTGCCACCATCTTTGCAAACATCTTGAAACTTGTCCTGAAAATTATAGATGGTACCAGACATTGAGTGGGGTAAAAATATTCGTGTTGTCCTGGACATTGGATGTACAGATTCAAGCTTCGCAGCTGCTCTCTTCGATAAGGGTGTTTTAACATTGTCACTTGGCTTGAAAGATGACCTAGTGGACTTAGCTCAAGTGGCACTCGAGCGCGGCTTCCCAACATTGGTTAGCCCTTTTGCTAGAAGGAGACTTCCTTTTCCAAGTCAAGTTTTTGATGCTATAAATTGTGCACGTTGCAGCATACCTTGGCATTCCAATGGTTTGTATTCCAGTCTATAATTTTGGTATTTTTAAGATGAAATTTTCTTCTACAGATTCTATTTATTTGATTATTACAACAATAGCTTGAACCATCCTTTCATTTTTTTTTCAGGGGGTAAACTTCTACTGGAGATGAATCGGATTCTAAGACCTGGTGGATACTTCATCATGTCAACTAAACATGATAGCATTGAAGAAGAAGAAGGTTTGTCTCAATTTTGAATAAAAAAGTGCACTCATTGATGAAATAAAGGAACTATGTTTCTTGTGATTCATTCTCGGTTTTTCCCCTCTGATTCTAAGCATGGTTATCATGGTACTGCTAGCTTGACACAAATTCTGTTTGCTTATCATATGTTTTAGCAAAAACACGATATTTAACCAGGTGTATGTAAAGGGTGGTACTAATGTTCGTTGAAAAATTGACTATGCAGTTTGTGTGATAAAACAAGAGTATTAACTGGGTCAAACAACCAAGTGTGAAGTCAAACAGTCACGAAAGAAAACAGTTAATTAAATACATAAGGACAAGAGAAACGCGTGGAAAAGAGACCTGTAATTGAAAACTGAATTACTTCACTTTAATGTTCTTGAATTGTTCATGACTGACATACATATTTATAAGCTCCTTACATAATTCTATCATTGACTTAATTGAGTTAACCTTTTAAATAATCACAAATCAAATAATGGTCATCTCCTAGAATACAAAATGCAATACATAAATCATGAACCTAGAATCAAACAAATGAACCTTGAAATAATAAATTCGTTATTCTTAAAATTTGAGTTGAGCCTAACTCAACCCTACAAAACCGACTTGTATGATGAGGATGTCTCTCACTTATAAAATCAATTTCGGTCCATATTTCATCTAATGTGAGACTCTTGTCACCTCCGACGCCCCATGAATGGTTACGCATGACTTGAGGGGCTTGATAATGGGTGTGGCCTAATGTACCAGCTTAGGTTTTTATGTGGGCTAATCCTGCTTACAAAATCGGCTTGTAGGGTGAGAGATGCCTCCCGCTTATAAACTCATTTTCAGACCTTATCTCGCCTAAGACTGAACATCTGAAGCGTGACCTGAGTAACCTTGAAACGGATGACTAACAACTTTTGTAGTGTGAGTTTATAAGTGGAAATGCATACAACTCACACAAAGAAAAGTAACATTTCTTCTCTGTTTCAGCCATGACCGCACTGACAGCCTCTATTTGTTGGAATGTTCTGGCACATAAAAGTGATGATGTTGGTGAAGTTGGAGTTAGAATATATCAAAAGCCTGAAGGAAATGACATATACGAATTGAGAAGGAAGAAGATCCCACCAATTTGCAAAGAAAATGAAAATCCCGACGCAGTCTGGTATGCTGCTGAAACATTAGTTGAGGACTTGTTTCTAATCAGCTTAAGGTTGTTTATTAATAATAGGTTATTTTTCATCATTTTTAAATAGGCATGTTCCAATCAAGACTTGTTTGCACACCATTCCAATTGGAATTGAACAGCATGGGGCAGAGTGGCCTGAGGAATGGCCGAAGAGGCTCGAAACTTTTCCTGACTGGATGAACAATAAGGAGAAATTGATCGCGGACACCAACCATTGGAACGCCATAGTTAACAAGTCATATCTCAATGGAATGGGCATTAACTGGACAAATATCAGGAATGTAATGGACATGAAATCCATCTATGGAGGGTATGCCAGCAAAACCACTTGATTTTAAGTTCGTTGCATCAATAAATGAGCTGCATTTTCCTTTGATACCATTCCGGACTTAAATATAAGGAAAAACCATACATGTATCTGGTCCAAATGTGAACCAAATACATGTACTTTTTTGCTTATATTTAGTACATCATTACATATTTAATACACATGTAATTTTATTCCATTGAATGATTTCCAACATCTTGTTAGAAACATGAATAAAATTTAATTAATTCTCCTATGAAAATACAGATTAGCTGCAGCTCTATCTAAACATAACGTTTGGGTGATGAATGTAGTACCTGTCCATGCACCGGATACGCTTCCCATAATTTTTGAACGGGGATTTTTTGGCGTCTATCACGATTGGTGCGAGTCTTTTGGTACTTATCCAAGAACTTATGACCTTCTGCATGCTGATCATTTGTTCTCACGCCTCAAAAGCAGGTATTCGGGAAATTTTACCAGTATTGTTGTACTTATCTTTCTCTTGATGTTCTTCCATAACCATTATACTAGCGTTTGCGTATAAGTTATTTCTATAGTTAAAATGGGAATATGATTGAACTGTTTTCGTATTTGTTGCAAGTTATTTCCATAAGTTACAATAGGAAGTTTGTTGAACTAAGCTGAAAAAACTTATGCACAGGTCATAATCTTGTAGACTATTTTTATAATGTATCTCGATCACTTACGCAGAGGTTTATGTCATAACATAAATCTTAATATGTAAATTAACTCTTGCAAATGCACTTAGTTGAGACCTGTGACCAAATTGCTTTTAACAATAAATTTGCAGGTGCAAGCAACCTGTGTCGATTGTTGTTGAGATGGACCGTATCTTACGGCCAGGAGGGTTGACGATCATACGTGATAAAGTTGAAGTCCTAAATGCATTGGAGGAAATATTGAGAAGTATGCAATGGGAAATCAGAATGACTTTCTACCAGGAAAAAGAGGGTATCTTATGTGCACAGAAAACTCTATGGAGGCCTTGA

Protein sequence

>MS.gene24281.t1

MAIIPRQAKRPTGLWVKMTAVTILGLCFIFVWTVFSSSSSSVSFQRESFEDISEPVSSSSSSSNVKKPTPKSEKRVEVEKDHKFHKSDEKKIDDGSSSNSSSTRPHKKEVAREKKRVHKKEDNNKENVNQGSLEKEEEGEKELEDKEEGLDHESEVDLDSDVVGGDDSAESVDEDSEILEELKKTKKGKVKGPLFESNVNYRWKMCNTRSKHNYIPCIDIEVGGGKVQYRHTERSCPRMPFMCMVPLPLDGYESPVPWPESKLKIFYKNVAHPKLAAYIKKHRWLVDSGEYLTFPQNQSEFLGGIQHYLESIEEMVPDIEWGKNIRVVLDIGCTDSSFAAALFDKGVLTLSLGLKDDLVDLAQVALERGFPTLVSPFARRRLPFPSQVFDAINCARCSIPWHSNGGKLLLEMNRILRPGGYFIMSTKHDSIEEEEAMTALTASICWNVLAHKSDDVGEVGVRIYQKPEGNDIYELRRKKIPPICKENENPDAVWHVPIKTCLHTIPIGIEQHGAEWPEEWPKRLETFPDWMNNKEKLIADTNHWNAIVNKSYLNGMGINWTNIRNVMDMKSIYGGLAAALSKHNVWVMNVVPVHAPDTLPIIFERGFFGVYHDWCESFGTYPRTYDLLHADHLFSRLKSRCKQPVSIVVEMDRILRPGGLTIIRDKVEVLNALEEILRSMQWEIRMTFYQEKEGILCAQKTLWRP